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Gene information
General information
TC000396 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12015710..12091087
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000396
    iBeetle screen iB_03015

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature dorsal partiallyirregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    larva partially potentiallynot closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva segment partially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000396 RA
      MKRRALATQ GKKAPSSWP IYSLVSTVA VACYLNGLN 
      GDFVHDDIP AVTLNKDVL AINPISHAF KNDFWGTPM 
      ADLASHKSY RPLTILTFR ANFLCFGLQ PVWFHATNV 
      VLHATACIL FTRVCLNIA AFKPPFATL AGLLFATHP 
      IHTEAVTGI VGRADVLAC VFFLVSLLA YHGHDGKCH 
      IWISVALGG LSMLAKETG VSVFLLNLA YDFYRHWPA 
      VKKTIVEVR WNQETQQFA NRAAKVLTS LGILLAIRL 
      ALLQGSLPK FSQQDNPTA FHPSLYVRV LTFCYLAAF 
      NWWLLLCPA TLSHDWQMG SIPLVTSLS DSRNLMTCF 
      FFGVAALLV IRSLCDFEN QKHIPVVLG LMLLILPFL 
      PAANLVVTV GFVVAERVL YIPSLGCTL LVVYGIQVL 
      WTSYSKHRQ TIVCCVMLL LTTNCLRTV VRNRDWRSR 
      ESLLRAGLM TLPHNAKMH YNYANFLRD SARPELAKS 
      HYHRALKLW PTYASAHNN LGTLLGNEQ EAEQHFLAA 
      IRYSADHVN AHYNLGQLY RKLNRTAES EHMLKRCIS 
      LEPRFTPAY IELARLRGP NDRSVNNLL KKVALLNPR 
      DPYYSTIYG HWLLGKGDN LQALQLYWR ALSVCTSYQ 
      EAMFGASKI LRKFGQSSR LFQLITRWQ SILRIRRGE 
      PPISPHVYL HGWQLKSEL SHKARAYDN CSTLLLGSR 
      CVQSSNKMK KIAPSEELG NKWTRQTSN KTKYHTMKQ 
      CKVQQKLHK QKPSTPLMV HHFLD
    CDS sequence
    • >TC000396 RA
      atgaagcga cgagcttta gctacgcaa gggaaaaaa 
      gcgcccagt tcgtggccc atatacagc ttagtgagc 
      accgttgca gtggcctgc tatcttaac ggacttaat 
      ggcgacttc gtgcacgat gatattcct gctgtgact 
      ctcaataag gacgtcttg gccatcaac cccatcagt 
      catgcattc aagaacgac ttctgggga acgccgatg 
      gccgacctc gccagccat aaatcctac agacctctc 
      accattctc actttcaga gcgaacttt ttgtgcttc 
      ggtctgcag ccagtgtgg tttcacgct acgaacgtc 
      gtcctgcat gccacagcc tgcatattg ttcaccagg 
      gtgtgcctg aacattgcc gccttcaag cccccgttc 
      gccaccctc gccggcctc ttattcgcc acccacccc 
      atccacacg gaagcggtg acgggtata gtgggcaga 
      gcagacgtt ttggcttgc gtcttcttc ttagtatcg 
      ctcctggca tatcacggg catgatggc aagtgtcat 
      atctggatc agcgtcgct ttgggaggc ctcagtatg 
      ttggcgaaa gagaccggc gtttcggtc tttttgctc 
      aatttggcg tacgacttt taccgccat tggcctgcc 
      gttaaaaaa acaatagtc gaagtgaga tggaatcag 
      gaaacccag caatttgct aatagagct gcgaaggtc 
      cttacatcg cttggtata ttacttgcc atcagactg 
      gctctttta caaggatcc ttacccaag ttttcgcaa 
      caggataat cccacagct ttccatccg tctctctat 
      gtgagagtg ttaactttc tgctacttg gcggcgttc 
      aactggtgg ctcctgctg tgtcctgcg acgctgtcg 
      catgattgg cagatgggg tcgataccc ctagtaacg 
      tcactctca gattcgagg aatctgatg acgtgcttc 
      ttcttcgga gtggcggca ctgcttgta ataagaagt 
      ctctgcgac tttgagaat cagaagcat attccggtg 
      gtgcttggc ctcatgttg ctcattttg ccgttcttg 
      ccggcagct aatctggtt gttactgta ggatttgta 
      gtggctgag agagtcttg tatattcct agcttaggt 
      tgtaccctc cttgtagta tatggaatc caagtcttg 
      tggacttct tattctaaa catcgccag acgatagtt 
      tgctgcgtg atgctgcta ttgaccaca aattgttta 
      cgcacggtc gtgcgtaat cgcgactgg cggtcccgc 
      gaatccctc ctgagggcc gggctaatg acattaccg 
      cacaacgcc aagatgcat tacaactac gcgaatttt 
      ctgcgggat tcggcgaga cctgaatta gcaaagtcc 
      cattaccac cgggctttg aagctgtgg cccacttat 
      gcaagcgcc cacaacaat ttgggcact ttgctgggc 
      aacgagcag gaagctgag cagcacttc ttggcggcc 
      atccgctac tcggccgac cacgtcaat gcgcactac 
      aatctggga cagctctat aggaaattg aatcgcacg 
      gctgaatcc gagcacatg ctgaagagg tgcatcagc 
      ctcgagccc cgcttcacg ccggcctac atcgaacta 
      gcaagactc agaggacca aacgaccga agcgtcaac 
      aatctactc aaaaaagtg gctttgctc aacccccga 
      gacccctac tacagcacc atttacggc cactggctg 
      ctgggaaaa ggcgacaat cttcaggcc ctccaactg 
      tactggagg gccctctcc gtgtgcacc tcctaccag 
      gaggcgatg ttcggagcc tcgaaaatt ttgcgtaaa 
      ttcggtcaa agttcacgc ctctttcaa ctgatcaca 
      cgatggcag tcaattttg cggatacgg agaggcgag 
      ccccccata agcccacac gtctattta cacggatgg 
      caacttaaa agcgaattg agccataaa gccagggcc 
      tacgacaat tgtagcacg ttgttactt ggtagtcgt 
      tgcgtacaa agttcaaat aaaatgaaa aaaatcgct 
      cctagtgaa gaactcgga aataaatgg acgaggcag 
      acaagcaac aagacgaag tatcacacg atgaagcag 
      tgcaaagtg caacaaaaa ctgcataaa cagaaacct 
      tccacgccg ctgatggtt caccatttc ctggattaa
    mRNA sequence
    • >TC000396 RA
      tcgagtcgc tcttcggtg atgacccat aaagagggt 
      gagctggtg cagttctca cttccgcca cagcagcgt 
      ggcgagcgc ggccatttg ggggtttga gggtagcag 
      tcgatagcg cgaatcgtt cgcaggcag tcgtgctgc 
      gcctctcct gcggtgagg tcctgacac cacctgatt 
      ttccgtgta atgtatgga aacgtgcag acgccatcc 
      cgaatacta atcatccga taagatctc gtaattatt 
      ccgtatgtt tctagttac gccctgcag aggatcctg 
      ccagttcct gcaagttgc gcattgctg gaccggcca 
      gtggcttcc aatacactt ccggtacgg tcgagataa 
      tcttcttca acaatgaac agtggtaga taaatgggc 
      gcataaaac taagcacgg agtgccacg ccgtgatca 
      atcgtggcc aaatgttcc attattgtt aaatggtgt 
      aagatttat cgttatgcc cgatcccga aaggccact 
      tgaattttt taaatagat caaaattta cgctaatcg 
      tgagtgaaa gcagaagtt atgttacga ctccgtggt 
      cttcataac cggcacgaa taaacattc attctgcgt 
      aataaagac agtgacctc gagattacg ccgagatat 
      gaagcgacg agctttagc tacgcaagg gaaaaaagc 
      gcccagttc gtggcccat atacagctt agtgagcac 
      cgttgcagt ggcctgcta tcttaacgg acttaatgg 
      cgacttcgt gcacgatga tattcctgc tgtgactct 
      caataagga cgtcttggc catcaaccc catcagtca 
      tgcattcaa gaacgactt ctggggaac gccgatggc 
      cgacctcgc cagccataa atcctacag acctctcac 
      cattctcac tttcagagc gaacttttt gtgcttcgg 
      tctgcagcc agtgtggtt tcacgctac gaacgtcgt 
      cctgcatgc cacagcctg catattgtt caccagggt 
      gtgcctgaa cattgccgc cttcaagcc cccgttcgc 
      caccctcgc cggcctctt attcgccac ccaccccat 
      ccacacgga agcggtgac gggtatagt gggcagagc 
      agacgtttt ggcttgcgt cttcttctt agtatcgct 
      cctggcata tcacgggca tgatggcaa gtgtcatat 
      ctggatcag cgtcgcttt gggaggcct cagtatgtt 
      ggcgaaaga gaccggcgt ttcggtctt tttgctcaa 
      tttggcgta cgactttta ccgccattg gcctgccgt 
      taaaaaaac aatagtcga agtgagatg gaatcagga 
      aacccagca atttgctaa tagagctgc gaaggtcct 
      tacatcgct tggtatatt acttgccat cagactggc 
      tcttttaca aggatcctt acccaagtt ttcgcaaca 
      ggataatcc cacagcttt ccatccgtc tctctatgt 
      gagagtgtt aactttctg ctacttggc ggcgttcaa 
      ctggtggct cctgctgtg tcctgcgac gctgtcgca 
      tgattggca gatggggtc gatacccct agtaacgtc 
      actctcaga ttcgaggaa tctgatgac gtgcttctt 
      cttcggagt ggcggcact gcttgtaat aagaagtct 
      ctgcgactt tgagaatca gaagcatat tccggtggt 
      gcttggcct catgttgct cattttgcc gttcttgcc 
      ggcagctaa tctggttgt tactgtagg atttgtagt 
      ggctgagag agtcttgta tattcctag cttaggttg 
      taccctcct tgtagtata tggaatcca agtcttgtg 
      gacttctta ttctaaaca tcgccagac gatagtttg 
      ctgcgtgat gctgctatt gaccacaaa ttgtttacg 
      cacggtcgt gcgtaatcg cgactggcg gtcccgcga 
      atccctcct gagggccgg gctaatgac attaccgca 
      caacgccaa gatgcatta caactacgc gaattttct 
      gcgggattc ggcgagacc tgaattagc aaagtccca 
      ttaccaccg ggctttgaa gctgtggcc cacttatgc 
      aagcgccca caacaattt gggcacttt gctgggcaa 
      cgagcagga agctgagca gcacttctt ggcggccat 
      ccgctactc ggccgacca cgtcaatgc gcactacaa 
      tctgggaca gctctatag gaaattgaa tcgcacggc 
      tgaatccga gcacatgct gaagaggtg catcagcct 
      cgagccccg cttcacgcc ggcctacat cgaactagc 
      aagactcag aggaccaaa cgaccgaag cgtcaacaa 
      tctactcaa aaaagtggc tttgctcaa cccccgaga 
      cccctacta cagcaccat ttacggcca ctggctgct 
      gggaaaagg cgacaatct tcaggccct ccaactgta 
      ctggagggc cctctccgt gtgcacctc ctaccagga 
      ggcgatgtt cggagcctc gaaaatttt gcgtaaatt 
      cggtcaaag ttcacgcct ctttcaact gatcacacg 
      atggcagtc aattttgcg gatacggag aggcgagcc 
      ccccataag cccacacgt ctatttaca cggatggca 
      acttaaaag cgaattgag ccataaagc cagggccta 
      cgacaattg tagcacgtt gttacttgg tagtcgttg 
      cgtacaaag ttcaaataa aatgaaaaa aatcgctcc 
      tagtgaaga actcggaaa taaatggac gaggcagac 
      aagcaacaa gacgaagta tcacacgat gaagcagtg 
      caaagtgca acaaaaact gcataaaca gaaaccttc 
      cacgccgct gatggttca ccatttcct ggattaaat 
      aaagtgctt tttcaaaat gttgttttc atattattg 
      tgttttgtg attttttgc tctaacttg taacaataa 
      tagacatta aaaagtaca aaatttcac tctttcgat 
      aaacaaaaa tctaaaatg actgctttg gtaaatatg 
      gtgattatg actgatttg taacaaatt attatatat 
      tataatttg tttttacct agtatataa taaagtttt 
      ttattccca aaaatgcca aactaccta ttcgtgata 
      cgtaataga aatctttgc agttttgta atttgttag 
      gtatttatt tgtgtttgt tatttgatt tgttcatga 
      tgtgaaggg tattaaata acattctaa tattaatat 
      tagtgagat taaccgaga gaggaattg atattaata 
      taaatcgca aaaattgaa ccatttatt gttataaac 
      taggtaggg acgcccaca tggtaataa tttgaatga 
      ctgcttgat ttcctagct tagttactc actccttta 
      gcagtatta ccgaaacgt ttcttgaat gactgaatt 
      tgataaaac aaagatatt tttaataaa ataattttg 
      taaagttct taaaaatgg tatgaacga accaagacc 
      ggtttacag tgtaaataa atattgcac gaaatgtaa 
      ataaaatta acagtatta caaaactct aggtctaag 
      tagcttagc aaattcact tttaaagca ttttataaa 
      acattgttg aagattaag gttccatat acacatttt 
      tttatgata ataaaatga atgtgatcg agtaaaaac 
      gttgttctc tccgtatat atagcttga tatttgtga 
      aatttttcc gctagtaaa attatggct aaaattatg 
      ttcgagaga tttgttttt agatggggc catttcatg 
      aactctgta tactgaata ttcatcagg cgaaactat 
      ttctcaaag tgaaattaa cgcaaacat acatattta 
      taaggttgt tgtgtctac aaattgtaa aatacttgt 
      atagttttt tgataatca ataaaatta tttattaac 
      a
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