Status
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Gene information
General information
TC000377 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12253240..12255497
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000377
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03013

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000377 RA
      MASSRDGRS SSASSHSSS NVPTNRSSI RTPKRQRST 
      AGGGSFEEK TTDFSCPVC FNLIEEAYI TKCGHTFCY 
      TCILKSIEA LKRCPKCNA PVTGEDMIF PNFLLNELI 
      RKHKTRLNN FEALGLNRD SSGEFGTSA DGLRDFVAT 
      ESQNLTLPD VNVMLEVLT QRKQLLEAE SCAAQNRLL 
      LEFLKHLLK QKKERQAQI IKEIAIIEH DIEEVYNKL 
      KEVHSKCPT LEDVERTVA SAADNADVS AMKKEMIDL 
      IDTIGSSTG RKDDTTNPD APLRLGSTS LAMRRRRMH 
      AHFDDFVDC YFTSRSKDL FFGKDGVDK TKTTINTGL 
      DEFRENLIK FSRYNSLRV LATLNYSSD LFNNSTIVS 
      SIEFDKDNE FLAIAGVTK KIKVFDYGA VIKDTVDIH 
      YPCIEMLSK SKISCVSWN TYHKSILAS SDYEGTVTI 
      WDASTGQRT KTYQEHEKR CWSVDFNEV DTRLIASGS 
      DDARVKLYS LNGEHSIAT LEAKANVCC VKFNPRSSC 
      HLAFGSADH CVHYYDLRN MKEAVAVFK GHKKAVSYV 
      KFLNSEDIV SASTDSQLK LWNVNTPYC LRSFVGHIN 
      EKNFIGLAT DGDYVACGS ENNAMYIYY KGLSKKLFS 
      YKFEAIQGV LDQERREDD MNEFVSAVC WKQNSNVVV 
      AANSQGIIN ILELV
    CDS sequence
    • >TC000377 RA
      atggcttca agccgcgac ggtcgtagt agttccgcc 
      tcttcgcac agtagttcg aacgtgccg acaaatcgc 
      tcgtcgatt cgcacccca aagcgtcag cgttcgaca 
      gccgggggc ggctccttc gaggagaaa acgaccgat 
      ttttcctgc cccgtctgc ttcaacctg atcgaggag 
      gcttacatt acgaaatgt ggccacacg ttctgctac 
      acctgcatt ttaaaatcg atcgaagcc ctcaaacgc 
      tgccccaaa tgcaacgcc ccggtcacc ggcgaagac 
      atgatcttc ccgaacttc ctcctcaac gaactgatc 
      agaaagcac aagactcgc ttgaacaac ttcgaggct 
      ttgggcttg aaccgcgac agttcgggg gagttcggg 
      acgtcggcc gacggttta cgggatttt gtggcaacg 
      gaatcgcag aatttaacg ttgccggac gttaacgta 
      atgttggag gttctgacg caacggaag caactcctg 
      gaggcggag tcgtgtgcc gcgcaaaac cgcctcctt 
      ttggagttt ttgaagcac ttgcttaag caaaagaag 
      gagcggcag gcgcagatt atcaaggag attgcgatt 
      attgagcat gacattgag gaggtttac aataagttg 
      aaggaggtg catagcaag tgcccgact ttggaggat 
      gtggagcgg acggtggcg tcggcggcg gacaatgcc 
      gatgtcagt gcgatgaag aaggaaatg atcgatttg 
      attgatact ataggtagt tcgactggt cgcaaagat 
      gacacgacg aatcccgac gccccgctt cggctaggt 
      tcgacctcg ctggcgatg cgccggcgg cggatgcac 
      gcccacttt gacgacttc gtcgactgt tacttcacc 
      tcacgctcc aaagacttg ttcttcggc aaagacggg 
      gtcgataag actaagact actattaat acgggtctc 
      gacgaattc cgggagaat ttgatcaaa ttctcgcgc 
      tataactcg ctccgggtc ctcgcaacg ttgaactac 
      tcgagtgat ttgttcaac aattcgacc atagtctcg 
      agtatcgaa ttcgataag gataacgag ttcctggcg 
      attgctgga gtgacgaaa aagatcaaa gttttcgat 
      tacggtgct gtgattaag gatacggtc gatatacac 
      tatccctgt attgaaatg ctctccaag tcgaaaatc 
      tcgtgcgtg agttggaac acgtaccat aagagtatt 
      ctggcgagt agtgattac gaagggact gtgacgatt 
      tgggacgct tcgaccggt cagcgcacc aagacctac 
      caagagcat gagaagcgc tgttggagt gttgatttc 
      aatgaggtt gatactcgg ttgatcgcc tcgggttcg 
      gacgatgcg cgcgttaag ttatactcg ctcaacggt 
      gagcactcg atcgcgact ctcgaagcc aaagcgaac 
      gtttgttgt gttaagttc aatccgagg agttcttgt 
      cacttagcg ttcggtagc gctgatcat tgtgttcat 
      tactacgac ttgaggaac atgaaggag gcggtggcc 
      gtgttcaag ggccacaag aaggcagtg agttacgtc 
      aagtttctc aatagtgag gatatcgtg tcggcgtcg 
      accgactcg cagcttaaa ttgtggaat gttaatact 
      ccgtattgc cttcggtcg tttgtcgga cacattaac 
      gagaagaat tttatcgga ttggcgacg gacggcgat 
      tatgtcgcc tgcggctcg gagaataac gccatgtat 
      atttattac aaaggcttg agtaaaaag ttgtttagt 
      tataagttt gaagcgatt caaggggtc ttggatcag 
      gaaaggcgg gaggatgat atgaacgaa ttcgtttcg 
      gctgtttgc tggaagcag aattcgaat gttgtcgtt 
      gcggcgaac agtcaaggg attattaat attttggaa 
      ctggtttag
    mRNA sequence
    • >TC000377 RA
      ttggccctt ttcgtgcaa attgaaaaa cctgacctg 
      aaacttgat cccgtcagt ttaaccaat ccagtagta 
      ttgagtgtt ttcaagtaa caatttagg cgatttctg 
      tccccgttt caggttttc gggtcagga accgtccga 
      attgtcgct cctcaatac tactccagt tcttgtttc 
      aggtcgatg tttttctct taactacct ctaagtcgt 
      caaaatggc ttcaagccg cgacggtcg tagtagttc 
      cgcctcttc gcacagtag ttcgaacgt gccgacaaa 
      tcgctcgtc gattcgcac cccaaagcg tcagcgttc 
      gacagccgg gggcggctc cttcgagga gaaaacgac 
      cgatttttc ctgccccgt ctgcttcaa cctgatcga 
      ggaggctta cattacgaa atgtggcca cacgttctg 
      ctacacctg cattttaaa atcgatcga agccctcaa 
      acgctgccc caaatgcaa cgccccggt caccggcga 
      agacatgat cttcccgaa cttcctcct caacgaact 
      gatcagaaa gcacaagac tcgcttgaa caacttcga 
      ggctttggg cttgaaccg cgacagttc gggggagtt 
      cgggacgtc ggccgacgg tttacggga ttttgtggc 
      aacggaatc gcagaattt aacgttgcc ggacgttaa 
      cgtaatgtt ggaggttct gacgcaacg gaagcaact 
      cctggaggc ggagtcgtg tgccgcgca aaaccgcct 
      ccttttgga gtttttgaa gcacttgct taagcaaaa 
      gaaggagcg gcaggcgca gattatcaa ggagattgc 
      gattattga gcatgacat tgaggaggt ttacaataa 
      gttgaagga ggtgcatag caagtgccc gactttgga 
      ggatgtgga gcggacggt ggcgtcggc ggcggacaa 
      tgccgatgt cagtgcgat gaagaagga aatgatcga 
      tttgattga tactatagg tagttcgac tggtcgcaa 
      agatgacac gacgaatcc cgacgcccc gcttcggct 
      aggttcgac ctcgctggc gatgcgccg gcggcggat 
      gcacgccca ctttgacga cttcgtcga ctgttactt 
      cacctcacg ctccaaaga cttgttctt cggcaaaga 
      cggggtcga taagactaa gactactat taatacggg 
      tctcgacga attccggga gaatttgat caaattctc 
      gcgctataa ctcgctccg ggtcctcgc aacgttgaa 
      ctactcgag tgatttgtt caacaattc gaccatagt 
      ctcgagtat cgaattcga taaggataa cgagttcct 
      ggcgattgc tggagtgac gaaaaagat caaagtttt 
      cgattacgg tgctgtgat taaggatac ggtcgatat 
      acactatcc ctgtattga aatgctctc caagtcgaa 
      aatctcgtg cgtgagttg gaacacgta ccataagag 
      tattctggc gagtagtga ttacgaagg gactgtgac 
      gatttggga cgcttcgac cggtcagcg caccaagac 
      ctaccaaga gcatgagaa gcgctgttg gagtgttga 
      tttcaatga ggttgatac tcggttgat cgcctcggg 
      ttcggacga tgcgcgcgt taagttata ctcgctcaa 
      cggtgagca ctcgatcgc gactctcga agccaaagc 
      gaacgtttg ttgtgttaa gttcaatcc gaggagttc 
      ttgtcactt agcgttcgg tagcgctga tcattgtgt 
      tcattacta cgacttgag gaacatgaa ggaggcggt 
      ggccgtgtt caagggcca caagaaggc agtgagtta 
      cgtcaagtt tctcaatag tgaggatat cgtgtcggc 
      gtcgaccga ctcgcagct taaattgtg gaatgttaa 
      tactccgta ttgccttcg gtcgtttgt cggacacat 
      taacgagaa gaattttat cggattggc gacggacgg 
      cgattatgt cgcctgcgg ctcggagaa taacgccat 
      gtatattta ttacaaagg cttgagtaa aaagttgtt 
      tagttataa gtttgaagc gattcaagg ggtcttgga 
      tcaggaaag gcgggagga tgatatgaa cgaattcgt 
      ttcggctgt ttgctggaa gcagaattc gaatgttgt 
      cgttgcggc gaacagtca agggattat taatatttt 
      ggaactggt ttaggtgat agtctagca cggcattag 
      gacaatcaa ctgttcttg gcatatcc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY