Status
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Gene information
General information
TC032203
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000367
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12319651..12330766
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032203
    iBeetle screen iB_03012

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032203 RA
      MEWLTQLLL VLPLMKFSN TLPDVIRIG GLFHPADDK 
      QEIAFRYAV EKINSDRMI LPRSKLSAQ IEKMSPQDS 
      FHASKKVCH LLRSGVAAI FGPQSAHTA SHVQSICDT 
      MEIPHLETR WDYRLRRES CLVNLYPHP TTLSKAYVD 
      LVKAWGWKS FTIIYENNE GLVRLQELL KAHGPYEFP 
      ITVRQLGES SDYRPLLKQ IKNSAESHI VLDCSTERI 
      YDVLKQAQQ IGMMSDYHS YLITSLDLH GVDLEEFKY 
      GGTNITAFR LVDPDGPEV RKVVREWNL SEAKNKKGE 
      ISSIIRAET ALMYDAVHL FAKALHDLD TSQQIDIKP 
      LSCDAVDTW PHGYSLINY MKIVEMRGL TGVIKFDHQ 
      GFRSDFVLD IIELNKEGL KKIGTWNST EGVNFTRTY 
      GEAYTQIVE IIQNKTFVV TTILSSPYV MRKEASEKL 
      TGNAQFEGY AVDLIHEIS RVLGFNYTI RLAPDGRYG 
      SLNRETKEW DGMIRELLD QKADLAIAD LTITYDREQ 
      AVDFTMPFM NLGISILYR KPIKQPPNL FSFLSPLSL 
      DVWIYMATA YLGVSVLLF ILARFTPYE WQNPHPCNP 
      NPDHLENQF TLFNCMWFA IGSLMQQGC DFLPKFSPY 
      EWDNPHPCN SDPDVLENQ FTLLNSLWF TIGSLMQQG 
      SDIAPKAVS TRMVAGMWW FFTLIMISS YTANLAAFL 
      TVERMDSPI ESADDLAKQ TKIKYGALR GGSTAAFFR 
      DSNFSTYQR MWSFMESQR PSVFTASNV EGVERVVKG 
      KGSYAFLME STSIEYVIE RNCELTQVG GMLDSKGYG 
      IAMPPNSPF RTAISGTIL KLQEEGKLH ILKTRWWKE 
      KRGGGACRD DTTKTSSTA NELGLANVG GVFVVLMGG 
      MGVACVIAV CEFVWKSRK VAVEERVSS ILHDT
    • >TC032203 RB
      MEWLTQLLL VLPLMKFSN TLPDVIRIG GLFHPADDK 
      QEIAFRYAV EKINSDRMI LPRSKLSAQ IEKMSPQDS 
      FHASKKVCH LLRSGVAAI FGPQSAHTA SHVQSICDT 
      MEIPHLETR WDYRLRRES CLVNLYPHP TTLSKAYVD 
      LVKAWGWKS FTIIYENNE GLVRLQELL KAHGPYEFP 
      ITVRQLGES SDYRPLLKQ IKNSAESHI VLDCSTERI 
      YDVLKQAQQ IGMMSDYHS YLITSLDLH GVDLEEFKY 
      GGTNITAFR LVDPDGPEV RKVVREWNL SEAKNKKGE 
      ISSIIRAET ALMYDAVHL FAKALHDLD TSQQIDIKP 
      LSCDAVDTW PHGYSLINY MKIVEMRGL TGVIKFDHQ 
      GFRSDFVLD IIELNKEGL KKIGTWNST EGVNFTRTY 
      GEAYTQIVE IIQNKTFVV TTILSSPYV MRKEASEKL 
      TGNAQFEGY AVDLIHEIS RVLGFNYTI RLAPDGRYG 
      SLNRETKEW DGMIRELLD QKADLAIAD LTITYDREQ 
      AVDFTMPFM NLGISILYR KPIKQPPNL FSFLSPLSL 
      DVWIYMATA YLGVSVLLF ILARFTPYE WQNPHPCNP 
      NPDHLENQF TLFNCMWFA IGSLMQQGC DFLPKFSPY 
      EWDNPHPCN SDPDVLENQ FTLLNSLWF TIGSLMQQG 
      SDIAPKAVS TRMVAGMWW FFTLIMISS YTANLAAFL 
      TVERMDSPI ESADDLAKQ TKIKYGALR GGSTAAFFR 
      DSNFSTYQR MWSFMESQR PSVFTASNV EGVERVVKG 
      KGSYAFLME STSIEYVIE RNCELTQVG GMLDSKGYG 
      IAMPPNSPF RTAISGTIL KLQEEGKLH ILKTRWWKE 
      KRGGGACRD DTTKTSSTA NELGLANVG GVFVVLMGG 
      MGVACVIAV CEFVWKSRK VAVEERSSL CAEMANELK 
      FAMRCQGST KPIRKKGRP CDASTGVDD ARFHPLGSY 
      TSYGFVVNK EPIN
    CDS sequence
    • >TC032203 RA
      atggagtgg ctaacgcaa ttgctttta gtgcttccg 
      ctaatgaaa ttttccaac actcttcca gacgtgatc 
      cgaataggt ggactgttt catcccgct gatgataag 
      caagagatc gcatttaga tatgcggtc gagaagatc 
      aacagcgat cggatgatc ctgccgagg tccaagctc 
      agcgcgcag atcgagaag atgtccccg caggacagc 
      ttccacgca tccaaaaaa gtttgtcac ctattgcga 
      agtggagtg gctgccatc ttcgggcct cagtcggca 
      cacacggcc agtcacgtg cagtcaata tgcgatacg 
      atggaaatc ccgcacctg gagacccgc tgggactac 
      cgcctccgg agggagagc tgtctagtc aacctgtac 
      ccccatccc accacgctg tccaaggcg tatgtggat 
      ttagtgaaa gcctggggc tggaaatcc ttcactata 
      atctacgag aataatgag ggtctggtg cggttgcag 
      gagctgttg aaagctcac ggtccctac gagtttccc 
      atcactgta cgccagctt ggggaaagc tctgattac 
      aggccactg ctgaagcag atcaagaac tcggcagag 
      tcgcatatc gttttagac tgttcgacg gagaggatt 
      tacgatgtt ttgaaacaa gcgcaacag atcggcatg 
      atgagcgac tatcacagc tatctcata acatcttta 
      gatctacac ggcgtagat ctggaggag ttcaaatac 
      ggaggtact aatataacg gctttccga ctcgtggat 
      cctgatgga ccggaagtt cgtaaagtt gtacgagaa 
      tggaatttg agcgaagca aagaacaaa aagggagaa 
      atttcatca ataattcgg gcagaaacg gccttaatg 
      tacgacgcg gtgcatttg tttgcgaaa gcactccat 
      gatttggac accagccaa caaatcgac atcaagccg 
      ctgagctgc gacgccgtg gatacctgg ccgcacggc 
      tacagtctc atcaactac atgaaaatc gtcgagatg 
      cggggcttg accggagtg atcaagttc gaccatcag 
      ggatttcgg agcgatttc gtgctggac atcatcgag 
      ctgaacaag gaggggctc aagaagatc ggcacgtgg 
      aactccacc gaaggtgtc aattttacg aggacttat 
      ggcgaggcc tacacccag atcgtggag attatccag 
      aataaaacg ttcgtcgtc accacgatc ctaagttct 
      ccgtacgtg atgcgtaaa gaggcgagt gagaaattg 
      acagggaac gcgcagttt gagggctac gccgtggat 
      ttaatccac gagatatcg cgagtgttg ggcttcaac 
      tacaccatc cggctggct cctgatggg agatacggg 
      tccctgaac cgggagacg aaggagtgg gacgggatg 
      atccgggaa ttgctggat cagaaagcc gacctggca 
      atcgccgac ttgactatc acttacgat agagaacaa 
      gctgtagat tttacgatg ccttttatg aacctaggc 
      atcagtatt ttgtaccga aagcccata aagcagcca 
      ccgaatctc ttctccttc ttatcgccg ctatcgctc 
      gacgtttgg atctacatg gcgacggcc tatctgggc 
      gtgtccgtg ctgctcttc atcctagcc aggttcact 
      ccctacgag tggcagaac ccacacccg tgcaaccct 
      aacccggac cacctcgag aaccagttc actttgttt 
      aattgcatg tggttcgct atcggttct ttaatgcag 
      caaggatgc gactttttg cccaagttc agtccgtac 
      gaatgggac aacccgcac ccatgcaat agcgatccg 
      gatgttcta gaaaaccag ttcactctc ttaaattcc 
      ttgtggttc accatcggg tctttgatg caacaagga 
      tctgacatt gcgcccaaa gccgtttcc acgagaatg 
      gtggccggg atgtggtgg ttcttcacc ttgatcatg 
      atttcctcg tatactgcc aacttggcc gcgtttttg 
      acggtggaa cgcatggac tcccccatc gaaagcgct 
      gacgatttg gccaaacag accaagatc aagtacggg 
      gcgctccgc gggggctcc actgctgcc ttcttccgg 
      gactcaaac ttctcgacc taccagcgg atgtggtcg 
      tttatggag tcgcaaaga ccgtcggtc ttcaccgcg 
      agtaacgtg gaaggggtg gagcgggtc gttaaaggc 
      aagggcagc tacgctttc cttatggaa tccacttcg 
      atcgaatac gtaattgag cgaaactgc gagctgacc 
      caagtggga ggaatgctc gattcgaag ggatacggc 
      atcgccatg cctccaaat tccccgttc aggactgca 
      attagtggc acgatatta aagttgcaa gaagaggga 
      aaactccac atattgaaa acacgctgg tggaaggag 
      aaacggggc ggaggagcg tgtcgggac gatacgacc 
      aaaaccagc agcacagct aatgaatta ggtctggcc 
      aacgtgggt ggggttttt gtcgtactc atgggcgga 
      atgggagta gcgtgcgtt atagcagtg tgcgaattt 
      gtatggaaa tcgcggaaa gttgcagtg gaagaaagg 
      gtgagcagc attctacac gacacgtag
    • >TC032203 RB
      atggagtgg ctaacgcaa ttgctttta gtgcttccg 
      ctaatgaaa ttttccaac actcttcca gacgtgatc 
      cgaataggt ggactgttt catcccgct gatgataag 
      caagagatc gcatttaga tatgcggtc gagaagatc 
      aacagcgat cggatgatc ctgccgagg tccaagctc 
      agcgcgcag atcgagaag atgtccccg caggacagc 
      ttccacgca tccaaaaaa gtttgtcac ctattgcga 
      agtggagtg gctgccatc ttcgggcct cagtcggca 
      cacacggcc agtcacgtg cagtcaata tgcgatacg 
      atggaaatc ccgcacctg gagacccgc tgggactac 
      cgcctccgg agggagagc tgtctagtc aacctgtac 
      ccccatccc accacgctg tccaaggcg tatgtggat 
      ttagtgaaa gcctggggc tggaaatcc ttcactata 
      atctacgag aataatgag ggtctggtg cggttgcag 
      gagctgttg aaagctcac ggtccctac gagtttccc 
      atcactgta cgccagctt ggggaaagc tctgattac 
      aggccactg ctgaagcag atcaagaac tcggcagag 
      tcgcatatc gttttagac tgttcgacg gagaggatt 
      tacgatgtt ttgaaacaa gcgcaacag atcggcatg 
      atgagcgac tatcacagc tatctcata acatcttta 
      gatctacac ggcgtagat ctggaggag ttcaaatac 
      ggaggtact aatataacg gctttccga ctcgtggat 
      cctgatgga ccggaagtt cgtaaagtt gtacgagaa 
      tggaatttg agcgaagca aagaacaaa aagggagaa 
      atttcatca ataattcgg gcagaaacg gccttaatg 
      tacgacgcg gtgcatttg tttgcgaaa gcactccat 
      gatttggac accagccaa caaatcgac atcaagccg 
      ctgagctgc gacgccgtg gatacctgg ccgcacggc 
      tacagtctc atcaactac atgaaaatc gtcgagatg 
      cggggcttg accggagtg atcaagttc gaccatcag 
      ggatttcgg agcgatttc gtgctggac atcatcgag 
      ctgaacaag gaggggctc aagaagatc ggcacgtgg 
      aactccacc gaaggtgtc aattttacg aggacttat 
      ggcgaggcc tacacccag atcgtggag attatccag 
      aataaaacg ttcgtcgtc accacgatc ctaagttct 
      ccgtacgtg atgcgtaaa gaggcgagt gagaaattg 
      acagggaac gcgcagttt gagggctac gccgtggat 
      ttaatccac gagatatcg cgagtgttg ggcttcaac 
      tacaccatc cggctggct cctgatggg agatacggg 
      tccctgaac cgggagacg aaggagtgg gacgggatg 
      atccgggaa ttgctggat cagaaagcc gacctggca 
      atcgccgac ttgactatc acttacgat agagaacaa 
      gctgtagat tttacgatg ccttttatg aacctaggc 
      atcagtatt ttgtaccga aagcccata aagcagcca 
      ccgaatctc ttctccttc ttatcgccg ctatcgctc 
      gacgtttgg atctacatg gcgacggcc tatctgggc 
      gtgtccgtg ctgctcttc atcctagcc aggttcact 
      ccctacgag tggcagaac ccacacccg tgcaaccct 
      aacccggac cacctcgag aaccagttc actttgttt 
      aattgcatg tggttcgct atcggttct ttaatgcag 
      caaggatgc gactttttg cccaagttc agtccgtac 
      gaatgggac aacccgcac ccatgcaat agcgatccg 
      gatgttcta gaaaaccag ttcactctc ttaaattcc 
      ttgtggttc accatcggg tctttgatg caacaagga 
      tctgacatt gcgcccaaa gccgtttcc acgagaatg 
      gtggccggg atgtggtgg ttcttcacc ttgatcatg 
      atttcctcg tatactgcc aacttggcc gcgtttttg 
      acggtggaa cgcatggac tcccccatc gaaagcgct 
      gacgatttg gccaaacag accaagatc aagtacggg 
      gcgctccgc gggggctcc actgctgcc ttcttccgg 
      gactcaaac ttctcgacc taccagcgg atgtggtcg 
      tttatggag tcgcaaaga ccgtcggtc ttcaccgcg 
      agtaacgtg gaaggggtg gagcgggtc gttaaaggc 
      aagggcagc tacgctttc cttatggaa tccacttcg 
      atcgaatac gtaattgag cgaaactgc gagctgacc 
      caagtggga ggaatgctc gattcgaag ggatacggc 
      atcgccatg cctccaaat tccccgttc aggactgca 
      attagtggc acgatatta aagttgcaa gaagaggga 
      aaactccac atattgaaa acacgctgg tggaaggag 
      aaacggggc ggaggagcg tgtcgggac gatacgacc 
      aaaaccagc agcacagct aatgaatta ggtctggcc 
      aacgtgggt ggggttttt gtcgtactc atgggcgga 
      atgggagta gcgtgcgtt atagcagtg tgcgaattt 
      gtatggaaa tcgcggaaa gttgcagtg gaagaaagg 
      tcgtccttg tgtgctgag atggcaaat gaattaaaa 
      ttcgccatg cgttgtcaa gggtcgacg aaaccaatt 
      cgcaagaag ggacgccct tgcgatgcc tcaacaggg 
      gttgatgac gctcggttt catcccttg ggatcttac 
      acgtcttac ggtttcgtt gtcaataaa gaacccatt 
      aattaa
    mRNA sequence
    • >TC032203 RA
      ctcagatca gttatgtaa taaattaac tctccccga 
      aatcaataa aaatatcct ccgagttgc ttaaaacag 
      ccgaaaagt tgtaaactt tccgggcgc aaataccgg 
      aaaccaggc cgataaatt tcatttttg attctcggc 
      acagcttgt cggccgttt tctttattt cgatcgggt 
      cgtttctgg tgtgtattt gatttttca catcgtgga 
      catatcccg tctttatca tcggagcgt tctggagcc 
      gaacagcgc cgactccgg gcaaccgga ggctgggaa 
      taaaatccc ggctgcagt gagggttgc gggtctgca 
      agagccact caataacgg ccgcgtcga tacgtttcg 
      agtgttgtg ctttcaaga aaaactgaa gaactgcag 
      atgttttca tgatggagt ggctaacgc aattgcttt 
      tagtgcttc cgctaatga aattttcca acactcttc 
      cagacgtga tccgaatag gtggactgt ttcatcccg 
      ctgatgata agcaagaga tcgcattta gatatgcgg 
      tcgagaaga tcaacagcg atcggatga tcctgccga 
      ggtccaagc tcagcgcgc agatcgaga agatgtccc 
      cgcaggaca gcttccacg catccaaaa aagtttgtc 
      acctattgc gaagtggag tggctgcca tcttcgggc 
      ctcagtcgg cacacacgg ccagtcacg tgcagtcaa 
      tatgcgata cgatggaaa tcccgcacc tggagaccc 
      gctgggact accgcctcc ggagggaga gctgtctag 
      tcaacctgt acccccatc ccaccacgc tgtccaagg 
      cgtatgtgg atttagtga aagcctggg gctggaaat 
      ccttcacta taatctacg agaataatg agggtctgg 
      tgcggttgc aggagctgt tgaaagctc acggtccct 
      acgagtttc ccatcactg tacgccagc ttggggaaa 
      gctctgatt acaggccac tgctgaagc agatcaaga 
      actcggcag agtcgcata tcgttttag actgttcga 
      cggagagga tttacgatg ttttgaaac aagcgcaac 
      agatcggca tgatgagcg actatcaca gctatctca 
      taacatctt tagatctac acggcgtag atctggagg 
      agttcaaat acggaggta ctaatataa cggctttcc 
      gactcgtgg atcctgatg gaccggaag ttcgtaaag 
      ttgtacgag aatggaatt tgagcgaag caaagaaca 
      aaaagggag aaatttcat caataattc gggcagaaa 
      cggccttaa tgtacgacg cggtgcatt tgtttgcga 
      aagcactcc atgatttgg acaccagcc aacaaatcg 
      acatcaagc cgctgagct gcgacgccg tggatacct 
      ggccgcacg gctacagtc tcatcaact acatgaaaa 
      tcgtcgaga tgcggggct tgaccggag tgatcaagt 
      tcgaccatc agggatttc ggagcgatt tcgtgctgg 
      acatcatcg agctgaaca aggaggggc tcaagaaga 
      tcggcacgt ggaactcca ccgaaggtg tcaatttta 
      cgaggactt atggcgagg cctacaccc agatcgtgg 
      agattatcc agaataaaa cgttcgtcg tcaccacga 
      tcctaagtt ctccgtacg tgatgcgta aagaggcga 
      gtgagaaat tgacaggga acgcgcagt ttgagggct 
      acgccgtgg atttaatcc acgagatat cgcgagtgt 
      tgggcttca actacacca tccggctgg ctcctgatg 
      ggagatacg ggtccctga accgggaga cgaaggagt 
      gggacggga tgatccggg aattgctgg atcagaaag 
      ccgacctgg caatcgccg acttgacta tcacttacg 
      atagagaac aagctgtag attttacga tgcctttta 
      tgaacctag gcatcagta ttttgtacc gaaagccca 
      taaagcagc caccgaatc tcttctcct tcttatcgc 
      cgctatcgc tcgacgttt ggatctaca tggcgacgg 
      cctatctgg gcgtgtccg tgctgctct tcatcctag 
      ccaggttca ctccctacg agtggcaga acccacacc 
      cgtgcaacc ctaacccgg accacctcg agaaccagt 
      tcactttgt ttaattgca tgtggttcg ctatcggtt 
      ctttaatgc agcaaggat gcgactttt tgcccaagt 
      tcagtccgt acgaatggg acaacccgc acccatgca 
      atagcgatc cggatgttc tagaaaacc agttcactc 
      tcttaaatt ccttgtggt tcaccatcg ggtctttga 
      tgcaacaag gatctgaca ttgcgccca aagccgttt 
      ccacgagaa tggtggccg ggatgtggt ggttcttca 
      ccttgatca tgatttcct cgtatactg ccaacttgg 
      ccgcgtttt tgacggtgg aacgcatgg actccccca 
      tcgaaagcg ctgacgatt tggccaaac agaccaaga 
      tcaagtacg gggcgctcc gcgggggct ccactgctg 
      ccttcttcc gggactcaa acttctcga cctaccagc 
      ggatgtggt cgtttatgg agtcgcaaa gaccgtcgg 
      tcttcaccg cgagtaacg tggaagggg tggagcggg 
      tcgttaaag gcaagggca gctacgctt tccttatgg 
      aatccactt cgatcgaat acgtaattg agcgaaact 
      gcgagctga cccaagtgg gaggaatgc tcgattcga 
      agggatacg gcatcgcca tgcctccaa attccccgt 
      tcaggactg caattagtg gcacgatat taaagttgc 
      aagaagagg gaaaactcc acatattga aaacacgct 
      ggtggaagg agaaacggg gcggaggag cgtgtcggg 
      acgatacga ccaaaacca gcagcacag ctaatgaat 
      taggtctgg ccaacgtgg gtggggttt ttgtcgtac 
      tcatgggcg gaatgggag tagcgtgcg ttatagcag 
      tgtgcgaat ttgtatgga aatcgcgga aagttgcag 
      tggaagaaa gggtgagca gcattctac acgacacgt 
      agctataag agaagtttt agttgatag ttttgtctc 
      caaaggcac aaaagtcgg ttgcgatcg caaacgcct 
      ttttgcctg gaagttttc gttacgtaa gctcaatac 
      tttgtaaat atgttgtac aattttata aagttttgt 
      ccgacatga tattccgcc attttgtaa cgtcaagtc 
      acgtgatct gcaatcgtt tagtacaca ttaacataa 
      acatagact gtacatact acgagcttt gttgtaaga 
      ccagggtcg ctcttttat ttagagaaa cgaatttat 
      cgacaatat cgaaccata atattgtca caatcaaca 
      aaatgtaac ttttatgac taaatactg taccaaaac 
      ggaccatta gcaagtttt tggtatggt atatgcagg 
      ttgttccag aaccaatta ctgttctgg aacacccgt 
      acaaacaac ttgtacgtg tgcggaact tagtgtgta 
      gccttttga tagataact atctaagtt tattttgaa 
      atatgtttt tgcttactt tgtattccg gaaaagtag 
      gaggtaagt gagccccta ccatccaga ttcctttgt 
      ttcgcccta aagtctctt acaggctta tctttcgta 
      aaatatctt tcccttgtg acgtatttt tcgattttt 
      cagtcgtcc ttgtgtgct gagatggca aatgaatta 
      aaattcgcc atgcgttgt caagggtcg acgaaacca 
      attcgcaag aagggacgc ccttgcgat gcctcaaca 
      ggggttgat gacgctcgg tttcatccc ttgggatct 
      tacacgtct tacggtttc gttgtcaat aaagaaccc 
      attaattaa aatatgata tattttgat tgccttgtt 
      aatcgttcc tgctgtttt gctacagac aatatgcgg 
      ctttttttc ggaccaagc tcagtacaa aatcatcaa 
      gggtttgct tcagttcaa aaacatatc attttttcg 
      gcttaattg aaatgtatc atattgggg ttagtagtt 
      aggtcttag ccgattgtt tcgttaaat actgccatg 
      ttacgtttg taaataatt tgttgtaaa aaatcatgt 
      ccatgttca taaatcagc aaattttca tcaataaac 
      tattccaac tgtt
    • >TC032203 RB
      ctcagatca gttatgtaa taaattaac tctccccga 
      aatcaataa aaatatcct ccgagttgc ttaaaacag 
      ccgaaaagt tgtaaactt tccgggcgc aaataccgg 
      aaaccaggc cgataaatt tcatttttg attctcggc 
      acagcttgt cggccgttt tctttattt cgatcgggt 
      cgtttctgg tgtgtattt gatttttca catcgtgga 
      catatcccg tctttatca tcggagcgt tctggagcc 
      gaacagcgc cgactccgg gcaaccgga ggctgggaa 
      taaaatccc ggctgcagt gagggttgc gggtctgca 
      agagccact caataacgg ccgcgtcga tacgtttcg 
      agtgttgtg ctttcaaga aaaactgaa gaactgcag 
      atgttttca tgatggagt ggctaacgc aattgcttt 
      tagtgcttc cgctaatga aattttcca acactcttc 
      cagacgtga tccgaatag gtggactgt ttcatcccg 
      ctgatgata agcaagaga tcgcattta gatatgcgg 
      tcgagaaga tcaacagcg atcggatga tcctgccga 
      ggtccaagc tcagcgcgc agatcgaga agatgtccc 
      cgcaggaca gcttccacg catccaaaa aagtttgtc 
      acctattgc gaagtggag tggctgcca tcttcgggc 
      ctcagtcgg cacacacgg ccagtcacg tgcagtcaa 
      tatgcgata cgatggaaa tcccgcacc tggagaccc 
      gctgggact accgcctcc ggagggaga gctgtctag 
      tcaacctgt acccccatc ccaccacgc tgtccaagg 
      cgtatgtgg atttagtga aagcctggg gctggaaat 
      ccttcacta taatctacg agaataatg agggtctgg 
      tgcggttgc aggagctgt tgaaagctc acggtccct 
      acgagtttc ccatcactg tacgccagc ttggggaaa 
      gctctgatt acaggccac tgctgaagc agatcaaga 
      actcggcag agtcgcata tcgttttag actgttcga 
      cggagagga tttacgatg ttttgaaac aagcgcaac 
      agatcggca tgatgagcg actatcaca gctatctca 
      taacatctt tagatctac acggcgtag atctggagg 
      agttcaaat acggaggta ctaatataa cggctttcc 
      gactcgtgg atcctgatg gaccggaag ttcgtaaag 
      ttgtacgag aatggaatt tgagcgaag caaagaaca 
      aaaagggag aaatttcat caataattc gggcagaaa 
      cggccttaa tgtacgacg cggtgcatt tgtttgcga 
      aagcactcc atgatttgg acaccagcc aacaaatcg 
      acatcaagc cgctgagct gcgacgccg tggatacct 
      ggccgcacg gctacagtc tcatcaact acatgaaaa 
      tcgtcgaga tgcggggct tgaccggag tgatcaagt 
      tcgaccatc agggatttc ggagcgatt tcgtgctgg 
      acatcatcg agctgaaca aggaggggc tcaagaaga 
      tcggcacgt ggaactcca ccgaaggtg tcaatttta 
      cgaggactt atggcgagg cctacaccc agatcgtgg 
      agattatcc agaataaaa cgttcgtcg tcaccacga 
      tcctaagtt ctccgtacg tgatgcgta aagaggcga 
      gtgagaaat tgacaggga acgcgcagt ttgagggct 
      acgccgtgg atttaatcc acgagatat cgcgagtgt 
      tgggcttca actacacca tccggctgg ctcctgatg 
      ggagatacg ggtccctga accgggaga cgaaggagt 
      gggacggga tgatccggg aattgctgg atcagaaag 
      ccgacctgg caatcgccg acttgacta tcacttacg 
      atagagaac aagctgtag attttacga tgcctttta 
      tgaacctag gcatcagta ttttgtacc gaaagccca 
      taaagcagc caccgaatc tcttctcct tcttatcgc 
      cgctatcgc tcgacgttt ggatctaca tggcgacgg 
      cctatctgg gcgtgtccg tgctgctct tcatcctag 
      ccaggttca ctccctacg agtggcaga acccacacc 
      cgtgcaacc ctaacccgg accacctcg agaaccagt 
      tcactttgt ttaattgca tgtggttcg ctatcggtt 
      ctttaatgc agcaaggat gcgactttt tgcccaagt 
      tcagtccgt acgaatggg acaacccgc acccatgca 
      atagcgatc cggatgttc tagaaaacc agttcactc 
      tcttaaatt ccttgtggt tcaccatcg ggtctttga 
      tgcaacaag gatctgaca ttgcgccca aagccgttt 
      ccacgagaa tggtggccg ggatgtggt ggttcttca 
      ccttgatca tgatttcct cgtatactg ccaacttgg 
      ccgcgtttt tgacggtgg aacgcatgg actccccca 
      tcgaaagcg ctgacgatt tggccaaac agaccaaga 
      tcaagtacg gggcgctcc gcgggggct ccactgctg 
      ccttcttcc gggactcaa acttctcga cctaccagc 
      ggatgtggt cgtttatgg agtcgcaaa gaccgtcgg 
      tcttcaccg cgagtaacg tggaagggg tggagcggg 
      tcgttaaag gcaagggca gctacgctt tccttatgg 
      aatccactt cgatcgaat acgtaattg agcgaaact 
      gcgagctga cccaagtgg gaggaatgc tcgattcga 
      agggatacg gcatcgcca tgcctccaa attccccgt 
      tcaggactg caattagtg gcacgatat taaagttgc 
      aagaagagg gaaaactcc acatattga aaacacgct 
      ggtggaagg agaaacggg gcggaggag cgtgtcggg 
      acgatacga ccaaaacca gcagcacag ctaatgaat 
      taggtctgg ccaacgtgg gtggggttt ttgtcgtac 
      tcatgggcg gaatgggag tagcgtgcg ttatagcag 
      tgtgcgaat ttgtatgga aatcgcgga aagttgcag 
      tggaagaaa ggtcgtcct tgtgtgctg agatggcaa 
      atgaattaa aattcgcca tgcgttgtc aagggtcga 
      cgaaaccaa ttcgcaaga agggacgcc cttgcgatg 
      cctcaacag gggttgatg acgctcggt ttcatccct 
      tgggatctt acacgtctt acggtttcg ttgtcaata 
      aagaaccca ttaattaaa atatgatat attttgatt 
      gccttgtta atcgttcct gctgttttg ctacagaca 
      atatgcggc tttttttcg gaccaagct cagtacaaa 
      atcatcaag ggtttgctt cagttcaaa aacatatca 
      ttttttcgg cttaattga aatgtatca tattggggt 
      tagtagtta ggtcttagc cgattgttt cgttaaata 
      ctgccatgt tacgtttgt aaataattt gttgtaaaa 
      aatcatgtc catgttcat aaatcagca aattttcat 
      caataaact attccaact gtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY