Status
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Gene information
General information
TC000361 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12453557..12474866
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000361
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06816

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    labrum not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    maxilla potentially orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    maxilla cuticle proximal irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head capsule potentiallysize decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head cuticle ventral structure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_03011

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    gena bristle number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    vertex vertex triplet anterior not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    labrum not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000361 RA
      MALGLGSGL SSGPGSGNH SETSSSPGI SVPIPVAPA 
      PLVPNPMFA LPTLNFTVA QVAAVCETL EESGDIERL 
      ARFLWSLPV AHPNIGELN KNEAVLRAR AIVCFHSGN 
      FRELYSILE SNKFSKSSH HKLQEIWQL AHYQEAEKL 
      RGRALGPVD KYRVRKKFP WPRTIWDGE QKTHCFKER 
      TRSLLREWY LQDPYPNPT KKRELAQAT GLTPTQVGN 
      WFKNRRQRD RAAAAKNRM QQQQLAAQL AHHGGGGRQ 
      PPLSPTPSD SDSDISLGA HSPPISSPG PMRFSNSPT 
      PITSFRFGP HSPGPMPPQ FRFGNHSPP GFRQDRKSR 
      NSSPINVDT TSNYNNSAV NLRLTSNES PIDVDSTQE 
      KSHSPIRVD SSPPPMNLR IHSDNTKIE TPLALRLTS 
      SHQLTTPLR IQVASPHRM AAPPSPPHT SLHLGTPHG 
      GIVRIAPPS PSNPPVLHR PFSPPRLT
    CDS sequence
    • >TC000361 RA
      atggcgctc ggactcggc tccggactg agctccggc 
      cccggctcc ggcaaccac tcggagacg tcctcgtcg 
      cccggcatc agcgtgccc atccccgtg gcgccggcg 
      ccgctcgtc cccaacccc atgttcgcg ctgcccacc 
      ctcaacttc accgtggcc caggtggcc gccgtctgc 
      gagaccctg gaggagagc ggcgacatc gagcggctg 
      gcgcgcttc ctctggtcg ctgccggtg gcccacccc 
      aacatcggc gagctcaac aagaacgag gccgtgctg 
      cgggcgcgc gccatcgtc tgcttccac agcggcaac 
      ttccgcgag ctgtactcc atcctggag tcgaacaag 
      ttcagcaag agctcgcac cacaagctg caggagatc 
      tggcagctg gcgcactac caggaggcg gagaagctc 
      cggggacgg gcgctgggg ccggtcgac aagtatcga 
      gtgcggaaa aagttcccg tggccgagg acgatctgg 
      gacggtgaa cagaaaacg cactgtttt aaggaaagg 
      actaggagt ttgttgcgg gagtggtat ctgcaggac 
      ccgtatccg aacccgacg aagaagagg gaattggcg 
      caagcgacg ggactgacg ccgacgcaa gtggggaac 
      tggttcaag aaccggagg cagcgggat cgagcggcc 
      gccgctaag aataggatg caacagcag cagctcgcc 
      gcccagctg gcccaccac ggtggcggc ggccggcag 
      ccgcccctc agccccacg ccgtccgac agcgactcg 
      gacatctcc ctcggagcg cactctccc cccatctcg 
      tccccaggt cccatgcgt ttttccaac tcccccaca 
      cccatcacc agttttcgt ttcgggccc cattctccc 
      ggccccatg cccccgcaa ttccgcttc ggcaatcac 
      tccccgccc ggcttccgt caagaccgc aaatcgagg 
      aactcgtcc ccaatcaac gtcgacacc acatcgaat 
      tacaataat tcagctgtc aatttgaga ttaacatcg 
      aacgagtcg cccatcgac gtcgattcc acgcaggag 
      aagagccac agccccatc agggtggac agctcgccg 
      ccgccgatg aatctgagg atacacagt gacaatacg 
      aagatcgag acgccgctg gcgctgcgg ctgacgtcg 
      agccaccag ctgacgacg ccgctgcgg atccaggtg 
      gcgtcgccg caccggatg gcggcgccg ccgtcgccg 
      ccccacacc agcctgcac ctggggacg ccccatgga 
      ggtatagtt agaatagca ccgccgagt ccctccaat 
      ccccccgtg cttcatcgg cctttctcg ccgccgaga 
      ctgacgtga
    mRNA sequence
    • >TC000361 RA
      tcgcctcag tgtaaccgc ggcctccac ctggcaagg 
      tgcgtgtga tggtgcact agcccaagc tggcaaccc 
      atatggcgc tcggactcg gctccggac tgagctccg 
      gccccggct ccggcaacc actcggaga cgtcctcgt 
      cgcccggca tcagcgtgc ccatccccg tggcgccgg 
      cgccgctcg tccccaacc ccatgttcg cgctgccca 
      ccctcaact tcaccgtgg cccaggtgg ccgccgtct 
      gcgagaccc tggaggaga gcggcgaca tcgagcggc 
      tggcgcgct tcctctggt cgctgccgg tggcccacc 
      ccaacatcg gcgagctca acaagaacg aggccgtgc 
      tgcgggcgc gcgccatcg tctgcttcc acagcggca 
      acttccgcg agctgtact ccatcctgg agtcgaaca 
      agttcagca agagctcgc accacaagc tgcaggaga 
      tctggcagc tggcgcact accaggagg cggagaagc 
      tccggggac gggcgctgg ggccggtcg acaagtatc 
      gagtgcgga aaaagttcc cgtggccga ggacgatct 
      gggacggtg aacagaaaa cgcactgtt ttaaggaaa 
      ggactagga gtttgttgc gggagtggt atctgcagg 
      acccgtatc cgaacccga cgaagaaga gggaattgg 
      cgcaagcga cgggactga cgccgacgc aagtgggga 
      actggttca agaaccgga ggcagcggg atcgagcgg 
      ccgccgcta agaatagga tgcaacagc agcagctcg 
      ccgcccagc tggcccacc acggtggcg gcggccggc 
      agccgcccc tcagcccca cgccgtccg acagcgact 
      cggacatct ccctcggag cgcactctc cccccatct 
      cgtccccag gtcccatgc gtttttcca actccccca 
      cacccatca ccagttttc gtttcgggc cccattctc 
      ccggcccca tgcccccgc aattccgct tcggcaatc 
      actccccgc ccggcttcc gtcaagacc gcaaatcga 
      ggaactcgt ccccaatca acgtcgaca ccacatcga 
      attacaata attcagctg tcaatttga gattaacat 
      cgaacgagt cgcccatcg acgtcgatt ccacgcagg 
      agaagagcc acagcccca tcagggtgg acagctcgc 
      cgccgccga tgaatctga ggatacaca gtgacaata 
      cgaagatcg agacgccgc tggcgctgc ggctgacgt 
      cgagccacc agctgacga cgccgctgc ggatccagg 
      tggcgtcgc cgcaccgga tggcggcgc cgccgtcgc 
      cgccccaca ccagcctgc acctgggga cgccccatg 
      gaggtatag ttagaatag caccgccga gtccctcca 
      atccccccg tgcttcatc ggcctttct cgccgccga 
      gactgacgt gatatatac cgtacgagt ggaaggaaa 
      aactgtgaa tatttcaaa ttttattcg gatttttgc 
      tgcaatatt tcattattt attgacact tgcttttaa 
      ctaaattgt gtatatgaa gtatacgtg tgcgagcga 
      actaattta tggactcta tcaattctg tgttcagtg 
      gcaatttgt agattgtat atggcatta cctatattt 
      caatttatg atagatcaa tttagtcca agtgcactg 
      aacgatgac tttttaagt acctaattc actttcttg 
      gcagtaaat attgtattt ttcggtcat gattgatga 
      ggttcagtg ttcagtctg ttgtataga aagagaaaa 
      ggcatgttc gcagtatat tttttttgt agatatata 
      ttttatttt aaggcgatt ttattgatt tctcgattg 
      tatataaaa tacatatta cacaatgac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY