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Gene information
General information
TC000356 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12574569..12577996
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000356
    iBeetle screen iB_03010

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000356 RA
      MDTSNISCP VCTLYLRPG ITLKTHLSS HPKQKVIEA 
      LVRLSETGD VEKIGNPSS QPDPGASTT HTAVVNQSW 
      NQGGLVNMP PLQGNHVFI YQQSMSSTT ASPQNVPSQ 
      YVIPTILNP QMMPYIYQQ QLIMSSGSC VSPMRTLPF 
      ELPSTSSQD CSITEEPEN ERDSPKKIE EIDEDASET 
      LSDSIKIDC DMQEQINNS LDLNVYEIE EGFNAQEGP 
      PSPDSESGE LNKACQTQN NNLTSEEDV SDETEPKQA 
      DSEFYFVNE NENPNYTSV ELGQNPIYT ASNILQSDQ 
      LDFVDMNGV QMVINDINE FAGNQIISQ VENFEGIAP 
      DRSGVLMTI GGMVDSAKD DFLDQRDAE ESMSRESSN 
      VNIRADERM PPRGELSGQ ESLGGSSDI TWNRVQYNE 
      GSSGMSTSY DLLTRENWE ASDSSDVEV PPLQSRIPS 
      HVTYPEEDD DSPTIVSFT GPPLNFKCS KCDEAFSCL 
      RERKEHEAE KHPEKRKLN RIGSELDKK KVKKLVVKL 
      KTEKPESEN NFVFTNKLK METPSEAET PEAQTDPIA 
      TTPQMPTEL KSVCPICGC VLKNAKALK EHKISVHKI 
      TALSRHKCI TCGELFPND YKFSIHLRI HPLECKICG 
      RLFYRRQNI QLHMRRHLG IKPFKCDVC QKAFLTKQK 
      LFEHRNIHT GEAPIKCPL CDETFRRHS NLVQHRNRH 
      HLNIKKKVK DYICFCKEV FHSKKKLAW HKEIHDAKP 
      KSCTHCSEK FIHMSSLTR HMRRAHNER FLPNGHKKA 
      ENVECPICK GVYLKSSLD VHIRNHSGQ RPYACLICN 
      KDFTTKWNL KLHKWTHAS RVSKPFKCD QCKGAFIRE 
      SDYIAHMNS HKSIRPYTC NHCGAQFIR KYNCQRHVK 
      EHENEKMFN CKVCGKSFH RSYYLKDHM RIHSGLRPY 
      SCHICGKTS TTKSNHNKH VQIHHAREP VSTEN
    CDS sequence
    • >TC000356 RA
      atggacacg agcaacata tcctgtcct gtctgtaca 
      ttgtatctc agaccgggc ataacccta aagacccac 
      ttgagcagc catcccaag cagaaggtg atcgaggct 
      ctggtccgg ctgtcggag accggagat gtggaaaaa 
      atcggcaac ccctcctcc cagcccgac cctggcgcc 
      tccaccacc cacaccgcc gtggtgaac cagagctgg 
      aaccagggc ggcttggtc aacatgccc cccctgcag 
      ggcaaccac gtcttcatc taccagcag agcatgagc 
      agcacgacc gcctcgccc caaaacgtg ccatcgcaa 
      tacgtcatt cccacgatt ttgaacccg caaatgatg 
      ccctacatc taccagcaa cagctgatc atgtcaagc 
      ggctcctgt gtttccccc atgcggacg ctgccgttt 
      gagttgccg tcgacgtcg tcacaggac tgcagcatc 
      acggaggag cccgagaac gaacgcgac tcgccgaag 
      aaaatcgag gagattgac gaagatgcg agcgagacg 
      ctgagtgat tcgattaaa atcgactgc gatatgcag 
      gagcagatc aataatagt ttggatttg aatgtgtat 
      gaaattgag gagggtttt aacgcacag gaagggccc 
      ccgagtccg gactccgag agcggcgag ttgaataaa 
      gcctgtcag acgcagaac aacaacttg acctcggaa 
      gaggacgtt tcggatgag actgagccg aaacaggcc 
      gattccgag ttttatttt gtgaacgag aacgagaat 
      ccgaattac acttcggtg gaattgggc cagaacccg 
      atttacacg gcttcgaat attttgcag tctgaccag 
      ctagacttc gttgatatg aacggagtc cagatggta 
      attaatgat ataaatgag tttgcgggc aatcagatt 
      atatctcag gttgaaaat tttgaaggt atcgctcct 
      gaccggtcg ggagtttta atgacgatc ggcgggatg 
      gttgatagt gctaaagat gattttctg gatcagaga 
      gatgcggaa gagagcatg tctcgggag agctcgaat 
      gttaacatc cgagccgac gagcgtatg cccccgagg 
      ggcgagctc tccggtcag gagagtcta gggggctcc 
      agtgatatc acctggaac cgggtgcaa tataacgaa 
      ggcagctca gggatgtcc acctcgtac gacctccta 
      acgcgcgaa aactgggag gcttccgac agttccgac 
      gtcgaagtg ccccccctt caaagtcgc atcccctca 
      cacgtgacc tatcccgag gaggacgac gacagcccc 
      actatagtc agcttcacc ggacctcct ctcaacttc 
      aaatgttcg aaatgtgac gaagcgttc agctgtctc 
      agagagagg aaagaacac gaggcggaa aaacatccc 
      gaaaaacgc aaacttaat cgaatcggc agcgaactt 
      gacaagaaa aaagtcaaa aaactcgtc gttaaactc 
      aaaactgaa aagcccgaa agcgagaac aacttcgtt 
      tttacgaac aaactcaaa atggaaact ccaagcgaa 
      gcggaaacg cccgaagca caaaccgat ccaatcgcc 
      acaacacca caaatgccg accgaattg aaatcggtt 
      tgtccgatc tgtggctgc gtactgaaa aatgccaaa 
      gcactgaag gagcataaa ataagcgtc cacaaaatt 
      acagcactg tcgcgccac aaatgtata acttgtggc 
      gaattgttc ccgaatgat tacaagttt tccatacat 
      ttgcgaata catccccta gaatgtaaa atatgtgga 
      cgcttgttc taccggaga cagaacatc cagctccat 
      atgaggcgc catttgggc ataaaaccc ttcaagtgt 
      gacgtgtgc cagaaagca tttctcacg aaacaaaaa 
      ctgttcgaa cacaggaac atccacacg ggcgaagcc 
      ccgatcaag tgtcccctg tgtgacgaa acgttccgc 
      cggcactcc aatttagtg cagcaccgg aaccggcac 
      cacctcaat atcaaaaag aaggtcaag gattacatc 
      tgtttctgc aaagaggtg ttccactcg aagaaaaag 
      ctcgcctgg cataaggaa atccacgat gccaagccc 
      aaatcttgc acccattgt agcgaaaag ttcatccac 
      atgtcgagc ttgacgcgt catatgcgc cgtgcgcat 
      aatgagcgc ttccttccg aacgggcat aagaaggcg 
      gaaaatgtg gagtgtcca atctgcaag ggggtttac 
      ctgaaatcg tccctggac gtccatata cgtaaccac 
      agcggccag aggccgtac gcgtgtctc atctgcaat 
      aaggacttc accacgaaa tggaacctc aagctgcac 
      aaatggacg cacgcgtcc cgcgtgtcc aaaccgttc 
      aaatgcgac caatgcaag ggtgctttc atccgcgag 
      tcggattac atcgctcac atgaactcg cataagagc 
      ataaggccg tatacgtgt aaccactgc ggggcgcag 
      ttcatccgc aaatacaac tgccagagg cacgtgaag 
      gagcacgag aatgagaag atgttcaat tgtaaggtg 
      tgcgggaag tcgttccac cgatcgtac tatttgaag 
      gaccacatg aggatccac agcgggctc aggccgtac 
      tcctgccac atttgcggc aagacttcg accactaag 
      tccaaccat aacaagcac gtgcagata caccatgcc 
      agagaaccg gtcagtact gagaactaa
    mRNA sequence
    • >TC000356 RA
      ttactgatt aaaatctca aaaaacttt aatttttcg 
      gaaaattgg tgtatctgc cgcctcctc aaaatgccg 
      ttttaaccc ggaaaagat caataacct agtcagagg 
      agtgtacag ctgagcaac ttgtgtgaa ttccaatca 
      gttgcaata aaaatggac acgagcaac atatcctgt 
      cctgtctgt acattgtat ctcagaccg ggcataacc 
      ctaaagacc cacttgagc agccatccc aagcagaag 
      gtgatcgag gctctggtc cggctgtcg gagaccgga 
      gatgtggaa aaaatcggc aacccctcc tcccagccc 
      gaccctggc gcctccacc acccacacc gccgtggtg 
      aaccagagc tggaaccag ggcggcttg gtcaacatg 
      ccccccctg cagggcaac cacgtcttc atctaccag 
      cagagcatg agcagcacg accgcctcg ccccaaaac 
      gtgccatcg caatacgtc attcccacg attttgaac 
      ccgcaaatg atgccctac atctaccag caacagctg 
      atcatgtca agcggctcc tgtgtttcc cccatgcgg 
      acgctgccg tttgagttg ccgtcgacg tcgtcacag 
      gactgcagc atcacggag gagcccgag aacgaacgc 
      gactcgccg aagaaaatc gaggagatt gacgaagat 
      gcgagcgag acgctgagt gattcgatt aaaatcgac 
      tgcgatatg caggagcag atcaataat agtttggat 
      ttgaatgtg tatgaaatt gaggagggt tttaacgca 
      caggaaggg cccccgagt ccggactcc gagagcggc 
      gagttgaat aaagcctgt cagacgcag aacaacaac 
      ttgacctcg gaagaggac gtttcggat gagactgag 
      ccgaaacag gccgattcc gagttttat tttgtgaac 
      gagaacgag aatccgaat tacacttcg gtggaattg 
      ggccagaac ccgatttac acggcttcg aatattttg 
      cagtctgac cagctagac ttcgttgat atgaacgga 
      gtccagatg gtaattaat gatataaat gagtttgcg 
      ggcaatcag attatatct caggttgaa aattttgaa 
      ggtatcgct cctgaccgg tcgggagtt ttaatgacg 
      atcggcggg atggttgat agtgctaaa gatgatttt 
      ctggatcag agagatgcg gaagagagc atgtctcgg 
      gagagctcg aatgttaac atccgagcc gacgagcgt 
      atgcccccg aggggcgag ctctccggt caggagagt 
      ctagggggc tccagtgat atcacctgg aaccgggtg 
      caatataac gaaggcagc tcagggatg tccacctcg 
      tacgacctc ctaacgcgc gaaaactgg gaggcttcc 
      gacagttcc gacgtcgaa gtgcccccc cttcaaagt 
      cgcatcccc tcacacgtg acctatccc gaggaggac 
      gacgacagc cccactata gtcagcttc accggacct 
      cctctcaac ttcaaatgt tcgaaatgt gacgaagcg 
      ttcagctgt ctcagagag aggaaagaa cacgaggcg 
      gaaaaacat cccgaaaaa cgcaaactt aatcgaatc 
      ggcagcgaa cttgacaag aaaaaagtc aaaaaactc 
      gtcgttaaa ctcaaaact gaaaagccc gaaagcgag 
      aacaacttc gtttttacg aacaaactc aaaatggaa 
      actccaagc gaagcggaa acgcccgaa gcacaaacc 
      gatccaatc gccacaaca ccacaaatg ccgaccgaa 
      ttgaaatcg gtttgtccg atctgtggc tgcgtactg 
      aaaaatgcc aaagcactg aaggagcat aaaataagc 
      gtccacaaa attacagca ctgtcgcgc cacaaatgt 
      ataacttgt ggcgaattg ttcccgaat gattacaag 
      ttttccata catttgcga atacatccc ctagaatgt 
      aaaatatgt ggacgcttg ttctaccgg agacagaac 
      atccagctc catatgagg cgccatttg ggcataaaa 
      cccttcaag tgtgacgtg tgccagaaa gcatttctc 
      acgaaacaa aaactgttc gaacacagg aacatccac 
      acgggcgaa gccccgatc aagtgtccc ctgtgtgac 
      gaaacgttc cgccggcac tccaattta gtgcagcac 
      cggaaccgg caccacctc aatatcaaa aagaaggtc 
      aaggattac atctgtttc tgcaaagag gtgttccac 
      tcgaagaaa aagctcgcc tggcataag gaaatccac 
      gatgccaag cccaaatct tgcacccat tgtagcgaa 
      aagttcatc cacatgtcg agcttgacg cgtcatatg 
      cgccgtgcg cataatgag cgcttcctt ccgaacggg 
      cataagaag gcggaaaat gtggagtgt ccaatctgc 
      aagggggtt tacctgaaa tcgtccctg gacgtccat 
      atacgtaac cacagcggc cagaggccg tacgcgtgt 
      ctcatctgc aataaggac ttcaccacg aaatggaac 
      ctcaagctg cacaaatgg acgcacgcg tcccgcgtg 
      tccaaaccg ttcaaatgc gaccaatgc aagggtgct 
      ttcatccgc gagtcggat tacatcgct cacatgaac 
      tcgcataag agcataagg ccgtatacg tgtaaccac 
      tgcggggcg cagttcatc cgcaaatac aactgccag 
      aggcacgtg aaggagcac gagaatgag aagatgttc 
      aattgtaag gtgtgcggg aagtcgttc caccgatcg 
      tactatttg aaggaccac atgaggatc cacagcggg 
      ctcaggccg tactcctgc cacatttgc ggcaagact 
      tcgaccact aagtccaac cataacaag cacgtgcag 
      atacaccat gccagagaa ccggtcagt actgagaac 
      taattgtac aattggata acctgtgat tcttttcgt 
      ggggctatc acggggtgg tagccgatt atgcattat 
      acaagtaat cgagagtac acttttaag taattttat 
      tttagctaa ttttatcgc attatggtt atcgatttt 
      aaggaccgc tagattaaa gactgtatt tatttattc 
      ttttatgta attttagac ttattggtg gactcaaat 
      ttttaaaca ttttattta taaaacggt cacttatgt 
      acatattac t
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