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Gene information
General information
TC000328 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13014285..13017927
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000328
    iBeetle screen iB_03007

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000328 RA
      MAESNGEMD DDIIINDQD GLPNVHPNL 
      LAEADIDSP EHVTDDEFE DLPTSLIVT 
      NIHDSVFTS PEKKREMEN LFRNFDPDV 
      TFQWLRSFR RLRVNFQTP VAAASARIQ 
      LHQYKINDS VITCYFAQP VTPVKNPSL 
      QPPAPFKQF LISPPASPP LDWEPRPEG 
      EPIINHDLL AALATLTPG ASHEVHPPS 
      PGQPSIVVH TALGADGVG AKGRMPQTS 
      CPERS
    CDS sequence
    • >TC000328 RA
      atggctgaa agcaacggc gaaatggac gacgatatc 
      atcattaac gaccaagac ggccttccc aacgtgcac 
      cccaacctg ctggcggag gccgacatc gacagcccc 
      gagcatgtg accgacgac gagttcgag gacctgccg 
      acgtccctc atcgtcacc aacatccac gacagtgtc 
      ttcacctcg cccgagaaa aagcgcgaa atggagaac 
      ctgttccgg aacttcgac ccggacgtg acattccag 
      tggctgcgc agtttccga agactgaga gtgaacttc 
      cagacgccc gtggcagcc gccagcgcc cgcattcag 
      ctccaccaa tacaaaatc aacgactca gtgattacg 
      tgttacttt gctcaaccc gtcaccccc gtcaaaaac 
      cccagcctg cagccgcca gcccccttc aagcagttc 
      ctaatctcg ccgccggcg tcgcccccc ctcgactgg 
      gagccccgg ccggaaggt gagcccatt atcaatcat 
      gaccttctt gcagctttg gccaccctc acgccgggg 
      gcgtcgcac gaggtgcac cccccctcc cccggccag 
      cccagcatc gtggtgcac acggccctg ggggcggac 
      ggcgtgggg gccaaaggc aggatgcca cagacgtcg 
      tgtccggaa cggagttaa
    mRNA sequence
    • >TC000328 RA
      caaaatgac ggattttat ttatcccaa ccacaagaa 
      attgcatta aacagcatt ttgtgactc atccccggg 
      ttgttattt tactgcgaa tggaaggaa agcaggaaa 
      accgtgttc gcacatata aacaccagc gataaaaat 
      tcaaagcgt ggaaagaaa ctttcagtc gccgataag 
      ttcttgcag agttaagtg tttttcctt cgctaataa 
      aaagcacaa tgtcgggcc ctacgctat tctcatttc 
      ctatcaaag gggctacta aaagttttg gaacgaccc 
      ttcaagtca cagacgatc aaaacgtac tcaaaattg 
      ggcagagtt tcactcccc cagctacta ccctcatca 
      gcgaaataa ccaacagta cggttactg cagaaactg 
      ctaccactc ctgagattt ctcaaaata ttacaacaa 
      cgattacca ccgtgataa ccgaagtga ccgacagta 
      cgaccgaaa caatcggta tttttgaaa acaacatca 
      cttcagcag tggccgaaa taaaaaaca gtacacaaa 
      atacaactt caaccccac ttgacgtat tttcttcaa 
      ctacggatg tttcgaacc aaatatatt acgaaggat 
      acgacgttg agggaaaaa ctttgtacg ttgttttgg 
      gagttataa tcgtcagtc tgctcttaa ttatcgtca 
      ttcttatcg tcgtttata agcgatata aagccagcc 
      gttcaggat cgcttgatt gtgcaaccg atttgagcc 
      aagttacca tctcaaacg gaagaatga aatcgtatt 
      aattatatt tgtgataat ctgttatat tttactatc 
      attatattt ttgttactc gcttaggta ggtttattt 
      atatcctca aaatgttat tgccgaaat aaattcttg 
      gtttataaa ttataatct aggtttttt attgaacac 
      gtccagttt ttcatgttt tatcagtta agtaatgaa 
      attactttt gtgtactgt gcagcacag acgtgacaa 
      aaaatttaa gttaaacac taaacaaaa catttttta 
      gcgatttgt ttcaaacaa aaattgttg aattgttac 
      tcacacgtt aacaatcat cacaattaa attttataa 
      ccatttaaa acaccaatt aacatttta tctgtaaaa 
      agtgtggtg atttgtatt gttgagtct gaatggtac 
      aaaacgctg ccacttttt cgatttttt ctggttaaa 
      aattaagaa cacaaacat tagtcaggc gttttgggc 
      aaaattaaa tctcattaa ttacggttt aaagggaaa 
      ataaataaa accgtgaat accaaataa caaaataga 
      aagaactta acctaactt tcttacggt tctaaaact 
      tacgtttat ttgccgggc ttccaaaag tcgactggt 
      ttgcatttc cgaacgaaa atgccataa ctgcattaa 
      atttaacgt tagtattta tataaaaat agtaattac 
      aatttttta tgtattcac aaattgaac ttaacgaat 
      tgacagaac tgtcaaact tcaaaccta ggttataca 
      ccggcaatt tcacaaaat ttcagcgtt ttctgtaca 
      aaaaaatac gaaatcaaa aagtcgctc atgtgttta 
      tgttaatta tctggaaat ctatccatt tcagggttt 
      tacgttttt ttaagctca ttcctttgt atttcaatt 
      cgggctggc gaactttcg gctttcatc cccttgtca 
      agtgtcatt cattcctga catttggca tttgcaatt 
      gttgggttg tgttttttg cactttgat attgcaatg 
      tttttgtgt taaaattta gtagcgtaa gagttagcc 
      ttgattagt ttagccgaa aaagtgcgc attgtttta 
      tggggttta aaccgcggt agatcctgt gtcggtgaa 
      tttaatcca atttttcaa tggctgaaa gcaacggcg 
      aaatggacg acgatatca tcattaacg accaagacg 
      gccttccca acgtgcacc ccaacctgc tggcggagg 
      ccgacatcg acagccccg agcatgtga ccgacgacg 
      agttcgagg acctgccga cgtccctca tcgtcacca 
      acatccacg acagtgtct tcacctcgc ccgagaaaa 
      agcgcgaaa tggagaacc tgttccgga acttcgacc 
      cggacgtga cattccagt ggctgcgca gtttccgaa 
      gactgagag tgaacttcc agacgcccg tggcagccg 
      ccagcgccc gcattcagc tccaccaat acaaaatca 
      acgactcag tgattacgt gttactttg ctcaacccg 
      tcacccccg tcaaaaacc ccagcctgc agccgccag 
      cccccttca agcagttcc taatctcgc cgccggcgt 
      cgccccccc tcgactggg agccccggc cggaaggtg 
      agcccatta tcaatcatg accttcttg cagctttgg 
      ccaccctca cgccggggg cgtcgcacg aggtgcacc 
      ccccctccc ccggccagc ccagcatcg tggtgcaca 
      cggccctgg gggcggacg gcgtggggg ccaaaggca 
      ggatgccac agacgtcgt gtccggaac ggagttaat 
      gatttgtac aggtcttca aggttattt taagtgtga 
      gcttcagag cctctcaat atgtcaatt tctctctat 
      gtagaatct gtcactcat ttattatgg aattacgtg 
      attcatttg ttggttgta actgtatca ttaatttaa 
      ctcaatgtg ataataaag gtgttgcta ataaaagat 
      aatttccta aagtactca gcgggaatt tgagacagg 
      ttggccctg agtttaatg ccatcagct ggcaacttc 
      aaacaggaa tctgtaatt atttattga agaaaacag 
      caaccaatt ttatattca acctagatt caagcaatt 
      gattatgaa attggggtg gaaaaaatt atcagaagt 
      actcagttt agaagaaat tttcttttt gtaattaca 
      aaactcgag aacttcctt tgtatgttt attaatgac 
      aacgaattt aaatgttta gttcatgct agtctttag 
      tctgtgatt ttttctctt ttcaccaga ttatttata 
      aatagtttc gtatattgt ttatttttt gcttacaat 
      ctttacacg agttacgaa attcggtaa ctttaaaaa 
      tgataaaac taaaatttt attggttta tttggttgt 
      gacaaaaat tatcgattt aaagttgct gcagtttcg 
      tcgaaaaca atattgatc aactttatg aataaaaaa 
      gagtgtaaa cgtaccaaa aatacggat acgtatttt 
      gtaaaatgg ctatgtata ttttgtata gtcgtaatg 
      tatgtccac tgactttct cgaattatt ctgcaatta 
      tgaagcttg aaattacat aattacgta tgggattaa 
      gtttatttt tagataatt tgtgcctgt ttccgacta 
      tttttcaag ctatatccc tgaacaata gttcgaaca 
      atgtccgta tccacctct ttatttcaa tggtaataa 
      aataaacaa aaagacgtt aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY