Status
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Gene information
General information
TC000325 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13026249..13028837
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000325
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03006

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000325 RA
      MTDKSKLLL LFDRPQEPA FMPKGNKEV FDVPNNFLP 
      ERYKPIGVQ IANRFGEQA NEKIPLKDI TPPDLEGIL 
      DLGRHENFS LFIPKHRKV AGKLIRIFL AAESIDDLL 
      SNAVFCRDR VNPYLFYYA FSVALLHRP DTQNLDLPS 
      FIHVFPDKY VDSQVFARA REEAHIVPE GSRTPIEIP 
      QDFTASDLD EEHRVAYWR EDIGLNLHH WHWHLVYPF 
      EGAREIVDK NRRGEIFYY MHQQIIARF NIERLCNGL 
      KRVVKLSNW REPLPEAYF PKLDSLVAS RTWPARPVN 
      QVPRDLNRE VDQIRLSLD DMDRWRDRI YDAIHSGVV 
      RGDNGQNIP LTEFEGIDV LGNIIESSI LSPNRNYYG 
      DFHNMGHIL IGYIHDPDH RFLEPFGVM ADPAVDLRD 
      PLFFRWHAY IDDMFQEFK ATLPRYTVA QLNYPGVTV 
      TNIEVQAKG GKPNVLSTF WQQSDLDLS RGLDFQPRG 
      SVFVRFTHL QNQDFTYKI TVNNSGNNR MGTCRIFLA 
      PQFDERGNP WLFRNQKDM FIELDRFAV SLKQGTNTI 
      TRHSTESSL TIPFERTFR NLDANRPTG GDALSEFNF 
      CGCGWPQHM LLPKGTEAG YPCHLFVMI SNYADDRVE 
      QDTSGTCSD GDTFCGIKD KLYPDRRSM GYPFDRMPR 
      DGVDTLQQF LTPNMRVQE VVIQFSNRI VKPRNRD
    CDS sequence
    • >TC000325 RA
      atgacggac aaaagcaaa ctcttgtta ctgttcgac 
      cgcccccaa gagcccgcc tttatgccc aaaggcaac 
      aaggaggtc ttcgacgtg cctaacaat ttcctcccg 
      gagcggtac aaaccgatt ggggtgcaa atcgccaac 
      cggttcggg gaacaagcc aacgagaaa atcccgttg 
      aaagacatc acaccgccg gacttggag ggaatcctt 
      gatctgggg cgccacgaa aacttctcc ctcttcatc 
      cccaaacac cgcaaggtt gccgggaag ctcatcagg 
      attttcctg gcggcggaa tcaatcgac gatcttcta 
      tcaaacgct gttttttgc agagatagg gttaatccg 
      tacttgttt tactatgcg ttttctgtg gcgcttttg 
      caccggccg gatacgcaa aatttggac ctcccgtcc 
      tttattcac gtgtttccg gataagtac gtcgacagt 
      caggtgttt gcaagggcc agggaggag gcccacatt 
      gtcccggaa ggctcaagg acccccatt gaaattccg 
      caagacttc acggcgtca gatttagac gaggagcac 
      cgggtggcc tactggcgc gaggacatc gggctgaac 
      ctccaccac tggcactgg cacctggtg tatcccttc 
      gaaggcgcg cgcgaaatt gtcgataaa aatcgccga 
      ggggaaatt ttctactac atgcaccaa caaatcatc 
      gcccgattc aacattgag cgactttgt aacggccta 
      aaacgagta gtgaagctg agcaattgg cgggagccg 
      ctccctgaa gcctatttc ccgaagttg gacagtctt 
      gtagctagt aggacctgg ccggcacga cccgttaac 
      caagtccca cgggacctc aaccgggaa gtcgaccag 
      attcgcctg agtctggac gacatggac cgctggagg 
      gacaggatc tacgacgcc atccattcg ggagtcgtg 
      cgcggggac aacggccag aacatccct ttgactgaa 
      tttgaaggg atcgatgtt ttagggaat ataattgag 
      tccagcatc ctgtcaccc aatcgcaac tattatggc 
      gatttccac aacatggga cacatcctc attggctat 
      atccacgac ccggaccat agattcttg gaaccgttc 
      ggagtcatg gcggatccg gccgttgat ttacgtgat 
      ccactcttc ttccgctgg cacgcctac attgacgac 
      atgttccag gagttcaag gccacgctt ccgcgatac 
      acggtggct caactgaat tatccggga gtgactgtc 
      accaacatc gaggtgcaa gccaagggg ggcaagccg 
      aacgtgctc agcactttc tggcaacaa tccgacttg 
      gatttgtct cgcggcttg gatttccag ccccggggc 
      tccgttttc gtccgcttc acccatttg caaaaccag 
      gacttcact tacaaaatc acagtcaac aactcgggg 
      aacaaccgg atgggaacc tgtcgcatt ttcctagcg 
      ccgcagttc gacgaacgg gggaatccc tggctgttc 
      aggaaccaa aaggacatg ttcatcgaa ctcgaccgt 
      tttgcagtt tccttgaaa caagggacg aatacgatc 
      actcgccac tccacggag tcctcccta actatcccc 
      ttcgagcgg acgttccgc aacttggac gcaaatcgc 
      cccacaggc ggggacgcc ctatccgag tttaatttc 
      tgcggctgt ggatggccc cagcacatg ttgttgccc 
      aaaggcacc gaggcgggc tatccgtgt cacttgttt 
      gttatgatt tcgaactac gctgatgat agggtcgag 
      caagatacg tcaggtacg tgcagtgat ggtgacact 
      ttctgcggg atcaaggat aagttgtac cccgatcgg 
      cgctccatg gggtatccc ttcgatcgc atgccgagg 
      gatggggtt gacaccctt caacagttt ctgaccccc 
      aatatgagg gtgcaagag gtggtgatc cagttcagt 
      aatcggatt gtgaagcca cgtaatcgt gattag
    mRNA sequence
    • >TC000325 RA
      tcggccgac ggcatttat ttaaacaat tcttgaagc 
      aacgcttca gtcttccac catgacgga caaaagcaa 
      actcttgtt actgttcga ccgccccca agagcccgc 
      ctttatgcc caaaggcaa caaggaggt cttcgacgt 
      gcctaacaa tttcctccc ggagcggta caaaccgat 
      tggggtgca aatcgccaa ccggttcgg ggaacaagc 
      caacgagaa aatcccgtt gaaagacat cacaccgcc 
      ggacttgga gggaatcct tgatctggg gcgccacga 
      aaacttctc cctcttcat ccccaaaca ccgcaaggt 
      tgccgggaa gctcatcag gattttcct ggcggcgga 
      atcaatcga cgatcttct atcaaacgc tgttttttg 
      cagagatag ggttaatcc gtacttgtt ttactatgc 
      gttttctgt ggcgctttt gcaccggcc ggatacgca 
      aaatttgga cctcccgtc ctttattca cgtgtttcc 
      ggataagta cgtcgacag tcaggtgtt tgcaagggc 
      cagggagga ggcccacat tgtcccgga aggctcaag 
      gacccccat tgaaattcc gcaagactt cacggcgtc 
      agatttaga cgaggagca ccgggtggc ctactggcg 
      cgaggacat cgggctgaa cctccacca ctggcactg 
      gcacctggt gtatccctt cgaaggcgc gcgcgaaat 
      tgtcgataa aaatcgccg aggggaaat tttctacta 
      catgcacca acaaatcat cgcccgatt caacattga 
      gcgactttg taacggcct aaaacgagt agtgaagct 
      gagcaattg gcgggagcc gctccctga agcctattt 
      cccgaagtt ggacagtct tgtagctag taggacctg 
      gccggcacg acccgttaa ccaagtccc acgggacct 
      caaccggga agtcgacca gattcgcct gagtctgga 
      cgacatgga ccgctggag ggacaggat ctacgacgc 
      catccattc gggagtcgt gcgcgggga caacggcca 
      gaacatccc tttgactga atttgaagg gatcgatgt 
      tttagggaa tataattga gtccagcat cctgtcacc 
      caatcgcaa ctattatgg cgatttcca caacatggg 
      acacatcct cattggcta tatccacga cccggacca 
      tagattctt ggaaccgtt cggagtcat ggcggatcc 
      ggccgttga tttacgtga tccactctt cttccgctg 
      gcacgccta cattgacga catgttcca ggagttcaa 
      ggccacgct tccgcgata cacggtggc tcaactgaa 
      ttatccggg agtgactgt caccaacat cgaggtgca 
      agccaaggg gggcaagcc gaacgtgct cagcacttt 
      ctggcaaca atccgactt ggatttgtc tcgcggctt 
      ggatttcca gccccgggg ctccgtttt cgtccgctt 
      cacccattt gcaaaacca ggacttcac ttacaaaat 
      cacagtcaa caactcggg gaacaaccg gatgggaac 
      ctgtcgcat tttcctagc gccgcagtt cgacgaacg 
      ggggaatcc ctggctgtt caggaacca aaaggacat 
      gttcatcga actcgaccg ttttgcagt ttccttgaa 
      acaagggac gaatacgat cactcgcca ctccacgga 
      gtcctccct aactatccc cttcgagcg gacgttccg 
      caacttgga cgcaaatcg ccccacagg cggggacgc 
      cctatccga gtttaattt ctgcggctg tggatggcc 
      ccagcacat gttgttgcc caaaggcac cgaggcggg 
      ctatccgtg tcacttgtt tgttatgat ttcgaacta 
      cgctgatga tagggtcga gcaagatac gtcaggtac 
      gtgcagtga tggtgacac tttctgcgg gatcaagga 
      taagttgta ccccgatcg gcgctccat ggggtatcc 
      cttcgatcg catgccgag ggatggggt tgacaccct 
      tcaacagtt tctgacccc caatatgag ggtgcaaga 
      ggtggtgat ccagttcag taatcggat tgtgaagcc 
      acgtaatcg tgattagtt ttatgacgt gaactgtct 
      gatttttta taatgtaat ctttgtaac gcttccgca 
      tattatgaa taaatctgc ttacgagtg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY