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Gene information
General information
TC000274 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13681652..13689467
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000274
    iBeetle screen iB_03003

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000274 RA
      MEEILSEGK NFRLLEDSE LPDIIEFIG QFLPHSIKF 
      HQTLKTYLN DRVWDFHFY VTKTWPDQP VILHFPGMT 
      STPNGKIYE SFSVFCPCD QLEHLELLA EEDVLIDWT 
      QPIYLNFTH ANIMNKIEE FYSQIGTIE KQYGDIYVL 
      SEPTKEEDS TNVVFAEDA EMLQLTPEY AQTIHDLYP 
      ANHMECVEI FEKLIEKLP CYGVFSASG DLAAWMVQS 
      YYGAMFSMQ TRPEYRRKG YGLILAKYL TKVVTERGY 
      LPFVVIRPE NDASKALYA KLGFKKHFE TVRAILRPH 
      DRANDEQGG GTEGQ
    CDS sequence
    • >TC000274 RA
      atggaagaa atattgtca gaggggaaa aatttcaga 
      ttacttgaa gactccgaa ctgccagac ataattgaa 
      tttattgga caatttttg cctcactca attaagttc 
      caccaaaca ctgaaaacc tatttgaac gaccgtgtt 
      tgggacttt catttctac gtgacgaaa acgtggcct 
      gatcaaccc gtgatcctc cactttcct ggcatgaca 
      agcacgcct aacgggaaa atttacgaa agcttcagt 
      gttttttgt ccttgcgac caactggag catttggaa 
      cttttagca gaagaagat gttttaatc gattggaca 
      caaccaatc tatctcaac tttacacac gctaacatt 
      atgaataaa atcgaagaa ttttacagt cagatagga 
      acgatagaa aaacaatat ggggacatt tatgtgctc 
      agcgaaccc accaaggag gaagacagt acaaatgtg 
      gtattcgca gaggatgcg gagatgctg cagttgact 
      cccgagtat gctcaaacc atccacgac ctataccca 
      gccaatcac atggaatgc gtggaaatt ttcgaaaaa 
      ctaatcgaa aagctgcct tgttacgga gttttctca 
      gcttcgggt gacctagca gcctggatg gtccaatct 
      tattacggt gcaatgttt tcgatgcaa acgcgacct 
      gaatacagg agaaaaggc tacggcctg atccttgcc 
      aagtacctg accaaagtg gtgacagaa aggggttat 
      ctgcctttc gtcgtcatc cgtcctgaa aacgacgca 
      tccaaagct ctgtacgca aaactaggg ttcaaaaaa 
      cacttcgaa actgtcagg gctatcctg cgcccccat 
      gatagggcc aacgacgag cagggggga gggacggag 
      gggcagtaa
    mRNA sequence
    • >TC000274 RA
      aagttaggt gcgtcgtgc gcgaccatt tcgcttccc 
      accaaatcc agtgtgagg gcagctgca gtacaaggg 
      gagcgctcg ggggctgca gacctctcc gcaccctgc 
      gagtcgatc aatgtgctt ttcctccta tcactcgtt 
      tgcaaatac tccggcaca cgagatata cattctttt 
      gctaaatcc ggtttctgt cacaattta aggacactg 
      cacgcagga ctagagcgg aggttttct tagattgct 
      cgtcttaaa cgagcaatg gaagaaata ttgtcagag 
      gggaaaaat ttcagatta cttgaagac tccgaactg 
      ccagacata attgaattt attggacaa tttttgcct 
      cactcaatt aagttccac caaacactg aaaacctat 
      ttgaacgac cgtgtttgg gactttcat ttctacgtg 
      acgaaaacg tggcctgat caacccgtg atcctccac 
      tttcctggc atgacaagc acgcctaac gggaaaatt 
      tacgaaagc ttcagtgtt ttttgtcct tgcgaccaa 
      ctggagcat ttggaactt ttagcagaa gaagatgtt 
      ttaatcgat tggacacaa ccaatctat ctcaacttt 
      acacacgct aacattatg aataaaatc gaagaattt 
      tacagtcag ataggaacg atagaaaaa caatatggg 
      gacatttat gtgctcagc gaacccacc aaggaggaa 
      gacagtaca aatgtggta ttcgcagag gatgcggag 
      atgctgcag ttgactccc gagtatgct caaaccatc 
      cacgaccta tacccagcc aatcacatg gaatgcgtg 
      gaaattttc gaaaaacta atcgaaaag ctgccttgt 
      tacggagtt ttctcagct tcgggtgac ctagcagcc 
      tggatggtc caatcttat tacggtgca atgttttcg 
      atgcaaacg cgacctgaa tacaggaga aaaggctac 
      ggcctgatc cttgccaag tacctgacc aaagtggtg 
      acagaaagg ggttatctg cctttcgtc gtcatccgt 
      cctgaaaac gacgcatcc aaagctctg tacgcaaaa 
      ctagggttc aaaaaacac ttcgaaact gtcagggct 
      atcctgcgc ccccatgat agggccaac gacgagcag 
      gggggaggg acggagggg cagtaaaca cttcttttt 
      tctatagca ggctgcgga cgcacagct tacaaccga 
      ttagctttt ttattgaga atttttcta ccgcttcca 
      aaattacat acagactgc aaaaattta ttccaaaac 
      ttcggtggg ctacaaaag tacaaactg cactttaaa 
      tcttctgca caggaaagg aaaggagaa tacaaatta 
      ctatctaca tatctctga cagttattt gctcagcgc 
      taaatagaa aataacacc aacgctaaa ttatgcatg 
      agcaacgaa tatttgctg caaattttt attttcttc 
      ctgttttgt gcagagaaa ttaaagaca aatgcacgt 
      tcacacaag tatacttca ctcgaactc ttcaagcaa 
      cgataattt acattagga ttgtgcgct acaattttt 
      gaagcccat caacttcac tcaaggctt tgtttgaca 
      aaatatctc taatataac tgattatta ctaaatgag 
      gaaattcta taactaacg taatattac tgcttaaag 
      ttgacaatt tgattggtt taaaaattg tttcggtca 
      caatgtatg acacctgtt tgtttttgt gtcgttcag 
      tgttttatt gtacagagt gttccgtcc aaacctcac 
      attagaagt attagtagt tctaataaa gatagtctt 
      ctgaaaatt tgtgtacaa ccatatttt cttaggtcc 
      tgcttaatg aaaatattt ttaagattt ttaatgcgt 
      ttttggatt tgagttatt ttaaaggcg tttttgtca 
      gctttcttt acacctaat gtgcgttta gggggttaa 
      gacggaaca ggctgtata ttggacgag ttcggattc 
      attggtcaa gttcgagaa aagtgtggg aattcgatt 
      cgacctttt aaacatcct tcctcaaaa ggctgtgct 
      attcataca ttagacaag aaaaagtca agtaaacct 
      tgtctcaat tttttcctc ggcaccttt cctcacatt 
      gtagtaacg aacacaaaa acaacatgg ttagaacac 
      aggcaagga aaaagtgta acgatgagc tacacgtgc 
      gtttcttct acaatgctc aaatgacgc gaccgcagc 
      atcaattcg gacttttcg ggcaatgtg cgtagtgtc 
      tacttatga attaacttt taatactgt gattaaaga 
      ctatcaaag actgaaaag ggaaacatt gtgtagtaa 
      ggactagtt agaatagag cagatgttt tatattttg 
      gcgcggttg cggtcgtgt cagttccaa cgcacttgt 
      agccgccag aaatcgttt agtgcttta aatccacca 
      tatccaatt tttctagga tggtgcaaa aacttagaa 
      tgaaatctt agggcgatt gtaagctgg ctgtttaac 
      ttcctgttg gcagtttca attttgtga aactttcaa 
      gatgtagtg caggccggc cattgtgat atttaattg 
      tagttataa tacagcaga aatgagctt tgattatac 
      gcactagag tatagtcaa agagatgta ttagttttt 
      gaaaactga agcgttgcc tatatcttt ttaaattat 
      tgcggcaaa gtgcgcttg ttgcccagt taaaaaaca 
      ttcccttta taaaataaa caaattggc gtttacggt 
      ttttaagat aacaaccat tccattcct ttatttaaa 
      ttgtgtgta atacttagg cctatagga gatagagag 
      acacatgat tagattgac caaggcgag ttccctaaa 
      tgcaaacga ggcaataat tatctaggg tttgcattt 
      ggggcaacc agccctcga atatatttt atttcatca 
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