General information | |||
TC032245
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 13815472..13862907 | |
Sequence information | |||
Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC032245 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
Name | ||
Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03000 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
beetle stock: bad (increased lethality),Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03001 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head segment random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC032245 RA
MKILTDFNN SNFVQYFEK GFTQSNFFK YLENHKFIT YNLNGSKSE KLIAQPTRE VADNKVFVV SLIIQIVML LALMFIIYL RLSSKNVAH KKIEMSQLD YGCLIYMAT QIASGMKYL ESLDLVHRD LAARNCVID KTFTVKISD HAMYCDRYE SDYYISDTK ARLPIRWMS WESLLLGRQ TTKSDVWAF AVTLWEVLM LCTQQPYAE LTSEQVVEN CNHWYQNDG CQRYLPRPA ACPREIYDL MGECWKRNA TDRPRFAEI HLFLQRKNL GYMPAPTQV
- >TC032245 RB
MGPLIFSLI RQEKNGGAW CPKAQISSE VREYLEVDL QKNHLVTWT ETQGRFGNG QGQEYAEAF LVEYWRASL NQWVIYKDS RGEKVLTGN SNTYLVVRQ RLELPFIAS KVRFIPYSE HPRTVCMRV ELYGCSWEQ NIMSYNAPR GESREPDVD LEDISYDGA LEGSQMRGG LGQLVDGLY GDDDYLKQL EGENSGSRW VGWNNDTRE GEPLDITFE FDSIREFSA VHLHANNMF TRGVQVFSQ AEIWFSLDG DRYQPNPIR ATVPADRAR ESARNITVQ LRGRPAKFI RLRLSFADK WILLSEISF ESVSLTNNL TEDIVEKYY LQPDGVSTT TAPGAPSGE TTHARKEVT PATSPTSSA QAYIGLITG VLAMVVLLL ACTVFLMIR RGRKKVALL HKHTALVSS STKPGVTIN MKDLKHMST PIINNGLSR SRLSVKSKV NTIGSVDSK KTKKSTLYG HVAGEESDS ENSSVYHEP YKLLPNAKQ EYGCLLKKD AISSSKSGE YTDFTSVNS FQEDLKFTS PSFYNLTPP PPPTSRPPT YDVQNYQTP TNTPNGTIP AENYYAATD IIKGERREQ HFTPGRFTP IKLPEGPPL EGVAHLLDF PRHRLRLLE KLGEGDFGM VHLCEAEGL PDFNGGSSF HKKQLVIVK SLWRGCGDT KRHEFLRET SWLAGLRDP NLARVVGLC SQDEPMCAL LEHSDPGDL PRFLAQRAV SEDGNQVGP TISYGCLIY MATQIASGM KYLESLDLV HRDLAARNC VIDKTFTVK ISDHAMYCD RYESDYYIS DTKARLPIR WMSWESLLL GRQTTKSDV WAFAVTLWE VLMLCTQQP YAELTSEQV VENCNHWYQ NDGCQRYLP RPAACPREI YDLMGECWK RNATDRPRF AEIHLFLQR KNLGYMPAP TQV
- >TC032245 RC
MGPLIFSLI RQEKNGGAW CPKAQISSE VREYLEVDL QKNHLVTWT ETQGRFGNG QGQEYAEAF LVEYWRASL NQWVIYKDS RGEKVLTGN SNTYLVVRQ RLELPFIAS KVRFIPYSE HPRTVCMRV ELYGCSWEQ NIMSYNAPR GESREPDVD LEDISYDGA LEGSQMRGG LGQLVDGLY GDDDYLKQL EGENSGSRW VGWNNDTRE GEPLDITFE FDSIREFSA VHLHANNMF TRGVQVFSQ AEIWFSLDG DRYQPNPIR ATVPADRAR ESARNITVQ LRGRPAKFI RLRLSFADK WILLSEISF ESVSLTNNL TEDIVEKYY LQPDGVSTT TAPGAPSGE TTHARKEVT PATSPTSSA QAYIGLITG VLAMVVLLL ACTVFLMIR RGRKKVALL HKHTALVSS STKPGVTIN MKDLKHMST PIINNGLSR SRLSVKSKV NTIGSVDSK KTKKSTLYG HVAGEESDS ENSSVYHEP YKLLPNAKQ EYGCLLKKD AISSSKSGE YTDFTSVNS FQEDLKFTS PSFYNLTPP PPPTSRPPT YDVQNYQTP TNTPNGTIP AENYYAATD IIKGERREQ HFTPGRFTP IKLPEGPPL EGVAHLLDF PRHRLRLLE KLGEGDFGM VHLCEAEGL PDFNGGSSF HKKQLVIVK SLWRGCGDT KRHEFLRET SWLAGLRDP NLARVVGLC SQDEPMCAL LEHSDPGDL PRFLAQRAV SEDGNQVGP TIR
- >TC032245 RA
atgaaaatc ttaactgat tttaacaat tctaatttc gttcaatat ttcgagaaa ggcttcact caaagtaat ttttttaaa tatttagaa aatcataaa tttataacg tacaatttg aacggaagc aagtcggaa aaactgata gcacaaccc acgcgagaa gtcgccgat aataaagta tttgtagtt agtctgatc attcaaatt gttatgtta ttagcgtta atgtttatt atctacctt aggctcagc tcgaaaaat gttgcacac aagaaaata gaaatgtct caattagat tacggctgt ctcatatac atggccacg cagattgct tccggaatg aagtactta gaatcctta gacttggtc cacagggat ttagccgca aggaattgc gtaatagac aaaacgttc acggtgaaa atatccgat catgcgatg tactgcgat agatacgaa tcagactac tacattagt gatacgaaa gctcggctt ccgatccgt tggatgtcg tgggaaagc ttactcctg ggccggcaa acgacaaaa agcgacgtg tgggctttt gccgtgact ttgtgggaa gtgttgatg ttgtgcacc caacagcct tacgccgaa ctgaccagc gagcaagtg gtcgagaac tgcaaccac tggtaccaa aacgacggc tgccagcgg tacctcccc cggccggcg gcgtgcccc cgggagatc tacgacctg atgggcgag tgctggaag cggaacgcc accgatcgg ccccgcttt gccgaaatc cacctcttc ctccagagg aaaaacctg ggctacatg ccggcaccc acccaagtg taa
- >TC032245 RB
atgggccct ttaatcttc tcgctgatc agacaagag aagaacgga ggtgcttgg tgtcctaaa gcccagatc agcagcgag gtgcgcgag tacctggag gtggacctg caaaagaac cacctcgtg acctggacg gagacccag ggccgtttc gggaacggc cagggtcag gaatacgcg gaagctttt ttagtagag tattggaga gcttcgtta aaccagtgg gtcatttat aaagactcc cgaggagag aaggtgcta actggcaac agcaacact tatctggtg gtgagacag cgtctggag ctgccgttc atcgccagc aaagtcaga tttattccg tacagcgag catccgagg accgtctgc atgcgtgtg gagctgtac ggatgctcc tgggagcaa aacatcatg agttacaac gcgcctcgg ggggaatcc cgggaacca gacgttgat ctggaggat atcagctac gatggggcg ctggagggc agccagatg aggggcggg ctggggcag ctggtggac gggctctac ggggacgac gactatctc aaacagcta gagggagag aactcagga agccgttgg gtgggatgg aataacgac actcgagag ggcgagccg ctcgatatt acttttgaa ttcgatagc ataagggaa ttcagtgct gttcacctc cacgccaac aacatgttc actcgtggg gtgcaggtg tttagccaa gcagagata tggttcagc ttggatggg gatcgttac cagccgaat ccgataagg gctacagtt ccggccgac cgtgcccgg gaatcagcc cggaacatc accgtgcaa cttcgaggt cgaccggcc aagtttatc aggctgaga ctgtctttt gcagacaag tggattctg ctcagcgaa atttccttc gagtcagtt tcgctcacc aacaacctc accgaagac atcgtcgag aagtattac ctccagccg gacggcgtc tccaccacc accgccccc ggcgccccc agcggggag accacccac gcccgcaag gaagtcacc cccgccacc agccccacg agcagcgct caggcctac atcggcctg ataacgggc gttctggcc atggtggtg cttttgtta gcctgcacc gtgttcctg atgataagg cgtggacgg aaaaaagtc gccctcctt cacaagcac accgcttta gtgtcgagc agcaccaag cccggggtc actatcaac atgaaggat ttgaagcat atgtccacc cctatcatc aacaatggc ctttcgagg tccagattg tccgtcaaa agcaaagtg aacacgata ggatcggtc gactccaag aagacgaaa aagagcacg ctttatgga cacgtggct ggagaggag tcagacagc gaaaattcc tcagtttat cacgagccc tacaaacta ctgccaaat gccaagcag gaatacggc tgcttgctc aagaaggac gccatttcg tccagcaaa agtggagag tatacagac ttcacgagc gtgaatagc ttccaagaa gatttaaaa ttcacgtcg ccttcgttt tacaacttg acgccgcca ccgccgccg acatcccgc ccgccgact tacgacgtc cagaattac caaacgcca acgaacaca ccgaacgga acgattcct gctgagaac tattacgcc gccacggat ataataaag ggtgaaagg cgcgagcaa catttcact ccgggccgg ttcaccccc attaaacta cccgaagga ccaccgctg gaaggggtg gcccacctt ctagacttt ccgagacat agactacgc ctcctcgag aagctagga gagggcgac tttggaatg gtgcatttg tgcgaagcc gaaggactt ccagacttc aatggaggc tcctcgttc cacaagaaa caactagtc atcgtcaaa agtctgtgg agaggctgt ggcgacacc aaaaggcac gagttcctg agggagacc agctggcta gcgggcctc agagacccg aatctggcc cgcgtggtg ggcctgtgc agccaggac gagccgatg tgcgccctt ctggagcac tcggacccg ggggacctg ccccgcttc ctggcgcag agggccgtc agcgaggac ggcaaccaa gttggacca actataagt tacggctgt ctcatatac atggccacg cagattgct tccggaatg aagtactta gaatcctta gacttggtc cacagggat ttagccgca aggaattgc gtaatagac aaaacgttc acggtgaaa atatccgat catgcgatg tactgcgat agatacgaa tcagactac tacattagt gatacgaaa gctcggctt ccgatccgt tggatgtcg tgggaaagc ttactcctg ggccggcaa acgacaaaa agcgacgtg tgggctttt gccgtgact ttgtgggaa gtgttgatg ttgtgcacc caacagcct tacgccgaa ctgaccagc gagcaagtg gtcgagaac tgcaaccac tggtaccaa aacgacggc tgccagcgg tacctcccc cggccggcg gcgtgcccc cgggagatc tacgacctg atgggcgag tgctggaag cggaacgcc accgatcgg ccccgcttt gccgaaatc cacctcttc ctccagagg aaaaacctg ggctacatg ccggcaccc acccaagtg taa
- >TC032245 RC
atgggccct ttaatcttc tcgctgatc agacaagag aagaacgga ggtgcttgg tgtcctaaa gcccagatc agcagcgag gtgcgcgag tacctggag gtggacctg caaaagaac cacctcgtg acctggacg gagacccag ggccgtttc gggaacggc cagggtcag gaatacgcg gaagctttt ttagtagag tattggaga gcttcgtta aaccagtgg gtcatttat aaagactcc cgaggagag aaggtgcta actggcaac agcaacact tatctggtg gtgagacag cgtctggag ctgccgttc atcgccagc aaagtcaga tttattccg tacagcgag catccgagg accgtctgc atgcgtgtg gagctgtac ggatgctcc tgggagcaa aacatcatg agttacaac gcgcctcgg ggggaatcc cgggaacca gacgttgat ctggaggat atcagctac gatggggcg ctggagggc agccagatg aggggcggg ctggggcag ctggtggac gggctctac ggggacgac gactatctc aaacagcta gagggagag aactcagga agccgttgg gtgggatgg aataacgac actcgagag ggcgagccg ctcgatatt acttttgaa ttcgatagc ataagggaa ttcagtgct gttcacctc cacgccaac aacatgttc actcgtggg gtgcaggtg tttagccaa gcagagata tggttcagc ttggatggg gatcgttac cagccgaat ccgataagg gctacagtt ccggccgac cgtgcccgg gaatcagcc cggaacatc accgtgcaa cttcgaggt cgaccggcc aagtttatc aggctgaga ctgtctttt gcagacaag tggattctg ctcagcgaa atttccttc gagtcagtt tcgctcacc aacaacctc accgaagac atcgtcgag aagtattac ctccagccg gacggcgtc tccaccacc accgccccc ggcgccccc agcggggag accacccac gcccgcaag gaagtcacc cccgccacc agccccacg agcagcgct caggcctac atcggcctg ataacgggc gttctggcc atggtggtg cttttgtta gcctgcacc gtgttcctg atgataagg cgtggacgg aaaaaagtc gccctcctt cacaagcac accgcttta gtgtcgagc agcaccaag cccggggtc actatcaac atgaaggat ttgaagcat atgtccacc cctatcatc aacaatggc ctttcgagg tccagattg tccgtcaaa agcaaagtg aacacgata ggatcggtc gactccaag aagacgaaa aagagcacg ctttatgga cacgtggct ggagaggag tcagacagc gaaaattcc tcagtttat cacgagccc tacaaacta ctgccaaat gccaagcag gaatacggc tgcttgctc aagaaggac gccatttcg tccagcaaa agtggagag tatacagac ttcacgagc gtgaatagc ttccaagaa gatttaaaa ttcacgtcg ccttcgttt tacaacttg acgccgcca ccgccgccg acatcccgc ccgccgact tacgacgtc cagaattac caaacgcca acgaacaca ccgaacgga acgattcct gctgagaac tattacgcc gccacggat ataataaag ggtgaaagg cgcgagcaa catttcact ccgggccgg ttcaccccc attaaacta cccgaagga ccaccgctg gaaggggtg gcccacctt ctagacttt ccgagacat agactacgc ctcctcgag aagctagga gagggcgac tttggaatg gtgcatttg tgcgaagcc gaaggactt ccagacttc aatggaggc tcctcgttc cacaagaaa caactagtc atcgtcaaa agtctgtgg agaggctgt ggcgacacc aaaaggcac gagttcctg agggagacc agctggcta gcgggcctc agagacccg aatctggcc cgcgtggtg ggcctgtgc agccaggac gagccgatg tgcgccctt ctggagcac tcggacccg ggggacctg ccccgcttc ctggcgcag agggccgtc agcgaggac ggcaaccaa gttggacca actataagg taa
- >TC032245 RA
tggttccct ttttatatt ttacactta attcacaaa atgtttaaa caattaaac ttgtctgaa acaaaatta atcataaag catgtgatt gataaatat gataattgt gtgggtgtt tccggtttt tgtacaaat gaagtaaat ctataattt attcatttt gtggtgtac gcacttatt aaaagttga ctctaataa gttaaatta aaagttatc ttaaaaaat tgtttaaag aaaaatgct ttatttttt aagcttgtt gttgattat attcttcat aaataaaac gaatgctga gtgccatca agatgatgc atataatga atattatca taggggacg tatctacta caatgggta caatcagag gaacgatat tccaaaaga agaaatact ttgcacaaa tttaagttc tattcagga catttttta ttctttcca aaccggatc ttatgagca aaatttagt aattgcagg ctcaaatat cacaaccat aaatccatc tgaactagc ccaattctc aacaataaa caaactatt tttaaatgg acaacacgt tgagaacat cttgccagg agctttcac cggtctcgt atctctgca agaactgca cctcaacga taacaactt aacaaaaat tctgaacgg tgtctttaa ttccttaca tagtttgat acttctgaa tttaaaaaa taacaaaat tcgacgaat tgaaacttt tgcttttat gggtatgtc taacttgaa acaattata tttaaatag gaataatat ttccgttat tgacaatgg aacttttga aaaattgca tatgttgac aaatcttga tgtatcgca caatccatt aaaacaatt tccttttgg tgatttgtc ggagattaa tgaactaca attgtcaaa aacagaatt gaatggaat agatcacga tttgtcaaa tttatcgat gaacttatt taatctgtc ttattgcca cttgtccac cttagactt tgcttattt cccacaagt taagaatct ttttttatc gcataatca aattcttgt cttggaaaa ttgtcaaaa gcttaatgc tacaaatgt tcgccttga ctataacaa aataaaaaa caccgatga aagttttaa tagcattcg actggattt aagtcataa cgaaatcgg aattctgaa aactttaaa accaaattc tgaaattct tgattttag ttataacaa catatccaa actagaaag ttgtgtttt ttgtaattt aacatctct acaacaatt aaacttacg acataatat tatatccaa aattaatcc taatttatt tcgcgactt gcaaaattt ggaagcgtt ggatttgtc ccagaacca aatcgaaga attttcgta cggactttt cgacagttt aatcagtct tgtaagttt aaacatttc caacaataa actaatcca gttaaatta cttacactt tctaccaaa tttacgaaa cttgtactt tgacaacaa tttaattac agcgtttga ttcaaaaga actagttca gcatttgcc aaaactggc attaatttc tttcaactc aaatccact atcatgtaa agaacttgt cgcagttgt tcattatct tcaaaccaa caaaattac cataatcga aggttattc atacgatct cgaaaacgt gcgaggtgt cgcttgtac taaatcaaa cacgtcttc tccatctgt ccaaaactt tatacaaaa ttttgaaaa ttctcttaa aagtatttc gttcaaagg catgatcca aaatttgca aaaatctgt aagtgatag tttggagaa aatgaaaat cttaactga ttttaacaa ttctaattt cgttcaata tttcgagaa aggcttcac tcaaagtaa tttttttaa atatttaga aaatcataa atttataac gtacaattt gaacggaag caagtcgga aaaactgat agcacaacc cacgcgaga agtcgccga taataaagt atttgtagt tagtctgat cattcaaat tgttatgtt attagcgtt aatgtttat tatctacct taggctcag ctcgaaaaa tgttgcaca caagaaaat agaaatgtc tcaattaga ttacggctg tctcatata catggccac gcagattgc ttccggaat gaagtactt agaatcctt agacttggt ccacaggga tttagccgc aaggaattg cgtaataga caaaacgtt cacggtgaa aatatccga tcatgcgat gtactgcga tagatacga atcagacta ctacattag tgatacgaa agctcggct tccgatccg ttggatgtc gtgggaaag cttactcct gggccggca aacgacaaa aagcgacgt gtgggcttt tgccgtgac tttgtggga agtgttgat gttgtgcac ccaacagcc ttacgccga actgaccag cgagcaagt ggtcgagaa ctgcaacca ctggtacca aaacgacgg ctgccagcg gtacctccc ccggccggc ggcgtgccc ccgggagat ctacgacct gatgggcga gtgctggaa gcggaacgc caccgatcg gccccgctt tgccgaaat ccacctctt cctccagag gaaaaacct gggctacat gccggcacc cacccaagt gtaacgcgg acggtttgt gtcgccgtg ttcggacgg acgtctcga tgtttcttt tccgatttt caatgttca caatacagg tattattta aggagtgat ttgtttctc gacattccc tcatgtact cggtaaaat aaggttgtt caagctttt ttcatgaat gtgaaccca ttgtgatat cgcacactg attttgtgc aaatgaaac catatcgtt ctatcaagt gtagatgac caaggcagc tgctcggta tgacggttt ttcgaagag aactgagta gaatcacgt caatgaggg agggccttc gcgaggaaa attgggtac cgaacagct acttcaata atttttaaa cctagactt aagaacctg tttcttaat tacgtgctt cctgtatgt gtcgactca cacacttta catggtatt ataattata tattaagtg tatttttcg gttggtttt tgtacaata ccatttttg gaaagtagt tcacctact tcgcggatt cacttacaa tgaaatgaa aatccttca attacccat ttcgggatg tactatatt ctagtcaga aatgtgtag gataagtct aaatattat aaattacga aaacgctgt tgtatggta tgtctttat tatgttgtg tacttaaga aaaatatat atttaactg ccgaaacac gtcaaattt gtgatccaa gagttaacg tagcgctga tttatggtt tctgacaaa cggctcaag tcgatctaa tttcgttcg atatatttt tctattttg cgttccaag aagtgtgat tattttgtt aggtatttg tatttttat aaggatcgt acttacata aataaaatt ttccagtgc cattgacct tgtaaataa gaacctttg taaataaat gataggatg aatgattca tattgtacc aaggatgta tacaggcat ggatatttt agatataaa tcttaaact taagcatag ttaggttta agtatgata acgtatttt ctataaaca cgatcttgt tggagaaac caataatta agtatacct acttctaaa ttccattcg acaagtgaa aattatttt ggttagcat gtgtgtatt gttattaaa aagtacaat ttacaattt tataatttc aaacacatg caaatcatc ttaatttaa tttaaaggc acgagacaa tcaagtgcg tgaatttaa gtcatgctt agaagcata gcacgttag tatttagcg cctttaaac ccggcattc gatttttca atgtgttgc acagttcag cttgtatca atcaacatc ttctaggac cacgtttct tattcaagt aataaatcg tttccatta caaaaaagt ttcgacaaa tttctattg tgtaatcac accctgttc gtaacacat aagcaacgc tctttatgt cgacgtttt tatatagat gtttaattc atacatatc acagataaa ttatttcca ttttcatta gcatgtgtc gatcgtaaa tgtgtttct gtaaataca tgagataat gttta
- >TC032245 RB
ctgacaaaa agaaattcg tatatggct actttgatg aattgtttg tgcctggaa tgggccctt taatcttct cgctgatca gacaagaga agaacggag gtgcttggt gtcctaaag cccagatca gcagcgagg tgcgcgagt acctggagg tggacctgc aaaagaacc acctcgtga cctggacgg agacccagg gccgtttcg ggaacggcc agggtcagg aatacgcgg aagcttttt tagtagagt attggagag cttcgttaa accagtggg tcatttata aagactccc gaggagaga aggtgctaa ctggcaaca gcaacactt atctggtgg tgagacagc gtctggagc tgccgttca tcgccagca aagtcagat ttattccgt acagcgagc atccgagga ccgtctgca tgcgtgtgg agctgtacg gatgctcct gggagcaaa acatcatga gttacaacg cgcctcggg gggaatccc gggaaccag acgttgatc tggaggata tcagctacg atggggcgc tggagggca gccagatga ggggcgggc tggggcagc tggtggacg ggctctacg gggacgacg actatctca aacagctag agggagaga actcaggaa gccgttggg tgggatgga ataacgaca ctcgagagg gcgagccgc tcgatatta cttttgaat tcgatagca taagggaat tcagtgctg ttcacctcc acgccaaca acatgttca ctcgtgggg tgcaggtgt ttagccaag cagagatat ggttcagct tggatgggg atcgttacc agccgaatc cgataaggg ctacagttc cggccgacc gtgcccggg aatcagccc ggaacatca ccgtgcaac ttcgaggtc gaccggcca agtttatca ggctgagac tgtcttttg cagacaagt ggattctgc tcagcgaaa tttccttcg agtcagttt cgctcacca acaacctca ccgaagaca tcgtcgaga agtattacc tccagccgg acggcgtct ccaccacca ccgcccccg gcgccccca gcggggaga ccacccacg cccgcaagg aagtcaccc ccgccacca gccccacga gcagcgctc aggcctaca tcggcctga taacgggcg ttctggcca tggtggtgc ttttgttag cctgcaccg tgttcctga tgataaggc gtggacgga aaaaagtcg ccctccttc acaagcaca ccgctttag tgtcgagca gcaccaagc ccggggtca ctatcaaca tgaaggatt tgaagcata tgtccaccc ctatcatca acaatggcc tttcgaggt ccagattgt ccgtcaaaa gcaaagtga acacgatag gatcggtcg actccaaga agacgaaaa agagcacgc tttatggac acgtggctg gagaggagt cagacagcg aaaattcct cagtttatc acgagccct acaaactac tgccaaatg ccaagcagg aatacggct gcttgctca agaaggacg ccatttcgt ccagcaaaa gtggagagt atacagact tcacgagcg tgaatagct tccaagaag atttaaaat tcacgtcgc cttcgtttt acaacttga cgccgccac cgccgccga catcccgcc cgccgactt acgacgtcc agaattacc aaacgccaa cgaacacac cgaacggaa cgattcctg ctgagaact attacgccg ccacggata taataaagg gtgaaaggc gcgagcaac atttcactc cgggccggt tcaccccca ttaaactac ccgaaggac caccgctgg aaggggtgg cccaccttc tagactttc cgagacata gactacgcc tcctcgaga agctaggag agggcgact ttggaatgg tgcatttgt gcgaagccg aaggacttc cagacttca atggaggct cctcgttcc acaagaaac aactagtca tcgtcaaaa gtctgtgga gaggctgtg gcgacacca aaaggcacg agttcctga gggagacca gctggctag cgggcctca gagacccga atctggccc gcgtggtgg gcctgtgca gccaggacg agccgatgt gcgcccttc tggagcact cggacccgg gggacctgc cccgcttcc tggcgcaga gggccgtca gcgaggacg gcaaccaag ttggaccaa ctataagtt acggctgtc tcatataca tggccacgc agattgctt ccggaatga agtacttag aatccttag acttggtcc acagggatt tagccgcaa ggaattgcg taatagaca aaacgttca cggtgaaaa tatccgatc atgcgatgt actgcgata gatacgaat cagactact acattagtg atacgaaag ctcggcttc cgatccgtt ggatgtcgt gggaaagct tactcctgg gccggcaaa cgacaaaaa gcgacgtgt gggcttttg ccgtgactt tgtgggaag tgttgatgt tgtgcaccc aacagcctt acgccgaac tgaccagcg agcaagtgg tcgagaact gcaaccact ggtaccaaa acgacggct gccagcggt acctccccc ggccggcgg cgtgccccc gggagatct acgacctga tgggcgagt gctggaagc ggaacgcca ccgatcggc cccgctttg ccgaaatcc acctcttcc tccagagga aaaacctgg gctacatgc cggcaccca cccaagtgt aacgcggac ggtttgtgt cgccgtgtt cggacggac gtctcgatg tttcttttc cgattttca atgttcaca atacaggta ttatttaag gagtgattt gtttctcga cattccctc atgtactcg gtaaaataa ggttgttca agctttttt catgaatgt gaacccatt gtgatatcg cacactgat tttgtgcaa atgaaacca tatcgttct atcaagtgt agatgacca aggcagctg ctcggtatg acggttttt cgaagagaa ctgagtaga atcacgtca atgagggag ggccttcgc gaggaaaat tgggtaccg aacagctac ttcaataat ttttaaacc tagacttaa gaacctgtt tcttaatta cgtgcttcc tgtatgtgt cgactcaca cactttaca tggtattat aattatata ttaagtgta tttttcggt tggtttttg tacaatacc atttttgga aagtagttc acctacttc gcggattca cttacaatg aaatgaaaa tccttcaat tacccattt cgggatgta ctatattct agtcagaaa tgtgtagga taagtctaa atattataa attacgaaa acgctgttg tatggtatg tctttatta tgttgtgta cttaagaaa aatatatat ttaactgcc gaaacacgt caaatttgt gatccaaga gttaacgta gcgctgatt tatggtttc tgacaaacg gctcaagtc gatctaatt tcgttcgat atatttttc tattttgcg ttccaagaa gtgtgatta ttttgttag gtatttgta tttttataa ggatcgtac ttacataaa taaaatttt ccagtgcca ttgaccttg taaataaga acctttgta aataaatga taggatgaa tgattcata ttgtaccaa ggatgtata caggcatgg atattttag atataaatc ttaaactta agcatagtt aggtttaag tatgataac gtattttct ataaacacg atcttgttg gagaaacca ataattaag tatacctac ttctaaatt ccattcgac aagtgaaaa ttattttgg ttagcatgt gtgtattgt tattaaaaa gtacaattt acaatttta taatttcaa acacatgca aatcatctt aatttaatt taaaggcac gagacaatc aagtgcgtg aatttaagt catgcttag aagcatagc acgttagta tttagcgcc tttaaaccc ggcattcga tttttcaat gtgttgcac agttcagct tgtatcaat caacatctt ctaggacca cgtttctta ttcaagtaa taaatcgtt tccattaca aaaaagttt cgacaaatt tctattgtg taatcacac cctgttcgt aacacataa gcaacgctc tttatgtcg acgttttta tatagatgt ttaattcat acatatcac agataaatt atttccatt ttcattagc atgtgtcga tcgtaaatg tgtttctgt aaatacatg agataatgt tta
- >TC032245 RC
ctgacaaaa agaaattcg tatatggct actttgatg aattgtttg tgcctggaa tgggccctt taatcttct cgctgatca gacaagaga agaacggag gtgcttggt gtcctaaag cccagatca gcagcgagg tgcgcgagt acctggagg tggacctgc aaaagaacc acctcgtga cctggacgg agacccagg gccgtttcg ggaacggcc agggtcagg aatacgcgg aagcttttt tagtagagt attggagag cttcgttaa accagtggg tcatttata aagactccc gaggagaga aggtgctaa ctggcaaca gcaacactt atctggtgg tgagacagc gtctggagc tgccgttca tcgccagca aagtcagat ttattccgt acagcgagc atccgagga ccgtctgca tgcgtgtgg agctgtacg gatgctcct gggagcaaa acatcatga gttacaacg cgcctcggg gggaatccc gggaaccag acgttgatc tggaggata tcagctacg atggggcgc tggagggca gccagatga ggggcgggc tggggcagc tggtggacg ggctctacg gggacgacg actatctca aacagctag agggagaga actcaggaa gccgttggg tgggatgga ataacgaca ctcgagagg gcgagccgc tcgatatta cttttgaat tcgatagca taagggaat tcagtgctg ttcacctcc acgccaaca acatgttca ctcgtgggg tgcaggtgt ttagccaag cagagatat ggttcagct tggatgggg atcgttacc agccgaatc cgataaggg ctacagttc cggccgacc gtgcccggg aatcagccc ggaacatca ccgtgcaac ttcgaggtc gaccggcca agtttatca ggctgagac tgtcttttg cagacaagt ggattctgc tcagcgaaa tttccttcg agtcagttt cgctcacca acaacctca ccgaagaca tcgtcgaga agtattacc tccagccgg acggcgtct ccaccacca ccgcccccg gcgccccca gcggggaga ccacccacg cccgcaagg aagtcaccc ccgccacca gccccacga gcagcgctc aggcctaca tcggcctga taacgggcg ttctggcca tggtggtgc ttttgttag cctgcaccg tgttcctga tgataaggc gtggacgga aaaaagtcg ccctccttc acaagcaca ccgctttag tgtcgagca gcaccaagc ccggggtca ctatcaaca tgaaggatt tgaagcata tgtccaccc ctatcatca acaatggcc tttcgaggt ccagattgt ccgtcaaaa gcaaagtga acacgatag gatcggtcg actccaaga agacgaaaa agagcacgc tttatggac acgtggctg gagaggagt cagacagcg aaaattcct cagtttatc acgagccct acaaactac tgccaaatg ccaagcagg aatacggct gcttgctca agaaggacg ccatttcgt ccagcaaaa gtggagagt atacagact tcacgagcg tgaatagct tccaagaag atttaaaat tcacgtcgc cttcgtttt acaacttga cgccgccac cgccgccga catcccgcc cgccgactt acgacgtcc agaattacc aaacgccaa cgaacacac cgaacggaa cgattcctg ctgagaact attacgccg ccacggata taataaagg gtgaaaggc gcgagcaac atttcactc cgggccggt tcaccccca ttaaactac ccgaaggac caccgctgg aaggggtgg cccaccttc tagactttc cgagacata gactacgcc tcctcgaga agctaggag agggcgact ttggaatgg tgcatttgt gcgaagccg aaggacttc cagacttca atggaggct cctcgttcc acaagaaac aactagtca tcgtcaaaa gtctgtgga gaggctgtg gcgacacca aaaggcacg agttcctga gggagacca gctggctag cgggcctca gagacccga atctggccc gcgtggtgg gcctgtgca gccaggacg agccgatgt gcgcccttc tggagcact cggacccgg gggacctgc cccgcttcc tggcgcaga gggccgtca gcgaggacg gcaaccaag ttggaccaa ctataaggt aaaatttgc gcgttccgc attgatttg tttggtttc aattgcgag aggaattag ccacgcatg ttcgtaata
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
