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Gene information
General information
TC032249
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000252
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid9: 149351..150638
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032249
    iBeetle screen iB_02999
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032249 RA
      MVLIFLFLA LLFNSAAAQ LRQFSNNLP SAKLEEDNL 
      DDTITQVFN KCKCVIFYL CDEENFIIP QDRVQITQT 
      TSNYSLKRV RRLRCSNEF YYCCKAKTQ ERGEKCGKS 
      NLASLRISD QFPWVVAIT EKKRYVNNF SFKSGGTLI 
      HPRVVLTAQ HNVLSVSSP NLLQVVAKG ETLNKLPAT 
      SLYLNVSKI IKHEGYYSG ALYNDIALL ILESAVTSI 
      NPVCLPNLN QKFDGQSCI AVGWSGTNR QQIKRLKKV 
      ELPIVESNK CEKLLRNTT LGHSFQVHP SFICAGGEE 
      GQDTCRGDG GGPLMCRLN NHFIQVGIV SWGLPICGQ 
      KDVPSLYTN VASLINWIR QKLEGENIY ISGYS
    CDS sequence
    • >TC032249 RA
      atggtatta attttccta tttttggcc cttttgttc 
      aactcagcc gcggcacaa ttgcgacag ttttcgaac 
      aatttgccg tccgcaaaa ttagaagaa gacaatctc 
      gacgacaca atcactcaa gttttcaac aagtgtaaa 
      tgtgtcatt ttttattta tgtgatgaa gaaaatttt 
      attatacca caggatcgg gtacaaata acgcaaact 
      acctccaac tattccttg aaacgtgtt cgccgctta 
      agatgctct aatgagttt tactactgt tgtaaagcg 
      aaaacacaa gaaaggggc gaaaaatgt ggcaaaagt 
      aatttagca agtttgcga atcagtgac caatttccg 
      tgggttgtg gcaatcacg gaaaaaaag cgctatgtt 
      aataatttt tcttttaaa agtgggggc actctgatt 
      caccccagg gtggtttta acagcacaa cacaatgta 
      ttaagtgtt tcctcgccg aatttatta caagtagtt 
      gcaaagggg gaaacttta aataaactt cctgcaaca 
      tccctttac ctcaacgtt tcgaaaata attaaacat 
      gagggttac tactctggg gccctttac aacgacatt 
      gctttactt attttagaa tcggccgtc acaagtatc 
      aaccccgtt tgtttacca aatttaaat caaaaattt 
      gatggacaa agttgtatt gctgttggt tggagtgga 
      acaaatcgt caacaaatc aaaagactg aaaaaagtc 
      gaattacca atcgttgaa tccaataaa tgtgaaaaa 
      cttctcaga aatacaact ttaggtcac agttttcaa 
      gtacatccg tcgtttatt tgtgccggg ggtgaggag 
      ggccaggat acatgtcgg ggggatgga gggggtcct 
      ttgatgtgt agactcaat aatcacttt attcaagtg 
      gggattgtt tcatggggt ttacctatt tgtggacag 
      aaagatgtg ccaagtttg tacaccaac gttgcgagt 
      ttgattaac tggattaga cagaagttg gaaggggaa 
      aatatctat atttcgggt tatagttaa
    mRNA sequence
    • >TC032249 RA
      cagttacaa ctctttgtt caagttaat taaaatggt 
      attaatttt cctattttt ggccctttt gttcaactc 
      agccgcggc acaattgcg acagttttc gaacaattt 
      gccgtccgc aaaattaga agaagacaa tctcgacga 
      cacaatcac tcaagtttt caacaagtg taaatgtgt 
      catttttta tttatgtga tgaagaaaa ttttattat 
      accacagga tcgggtaca aataacgca aactacctc 
      caactattc cttgaaacg tgttcgccg cttaagatg 
      ctctaatga gttttacta ctgttgtaa agcgaaaac 
      acaagaaag gggcgaaaa atgtggcaa aagtaattt 
      agcaagttt gcgaatcag tgaccaatt tccgtgggt 
      tgtggcaat cacggaaaa aaagcgcta tgttaataa 
      tttttcttt taaaagtgg gggcactct gattcaccc 
      cagggtggt tttaacagc acaacacaa tgtattaag 
      tgtttcctc gccgaattt attacaagt agttgcaaa 
      gggggaaac tttaaataa acttcctgc aacatccct 
      ttacctcaa cgtttcgaa aataattaa acatgaggg 
      ttactactc tggggccct ttacaacga cattgcttt 
      acttatttt agaatcggc cgtcacaag tatcaaccc 
      cgtttgttt accaaattt aaatcaaaa atttgatgg 
      acaaagttg tattgctgt tggttggag tggaacaaa 
      tcgtcaaca aatcaaaag actgaaaaa agtcgaatt 
      accaatcgt tgaatccaa taaatgtga aaaacttct 
      cagaaatac aactttagg tcacagttt tcaagtaca 
      tccgtcgtt tatttgtgc cgggggtga ggagggcca 
      ggatacatg tcgggggga tggaggggg tcctttgat 
      gtgtagact caataatca ctttattca agtggggat 
      tgtttcatg gggtttacc tatttgtgg acagaaaga 
      tgtgccaag tttgtacac caacgttgc gagtttgat 
      taactggat tagacagaa gttggaagg ggaaaatat 
      ctatatttc gggttatag ttaataaag acaaatcta 
      caatctaag tatttattt gaatacga