Status
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Gene information
General information
TC000244 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14209645..14242851
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000244
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02998

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Fertility
    vitellarium decreased - Number of animals showing the phenotype: 3

    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 3
    vitellogenic egg chamber early not present - Number of animals showing the phenotype: 3
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000244 RA
      MRQGRAGQR KARCLSRRV APPITLPVT LEDPATTWK 
      GPPPGSEPQ NFATNAANS FSGPPAGFQ GPSPFQGPP 
      AGAGSPAGA PPYQGGPPP GSSTPQYTA SPAPSGSST 
      PGPGPPQSV GNAFPPPPN SAGPPYNGP GPGPFGSPS 
      AGGPPFGRP GSSGPPFVG GPGPGGNPH FASPGQPFA 
      PGFGIPPGS PFGPGPGHP MSGPMEPGH GIMARQIPG 
      PIPVDQGSL PVPRRHTPY FGQPDYRIY ELNKRLQQR 
      TEESDNLWW DAFATEFFE DDASLTLAF CLEDGPKRY 
      TIGRTLIPR YFRSIFEGG VTELYYNMK HPKESFHNT 
      SITLDCDQC TMITHHGKP LFTKVCTEG RLILEFTFD 
      DLMRIKSWH FAVRTHREL IPRSVVGMH SQQDPSMLD 
      QLSKNITRQ GITNSTLNY LRLCVILEP MQELMSRHK 
      AYALSPRDC LKTTLFQKW QRMVAPPES QRPANKRRK 
      RKGSNSGGA PNNATPTPN KKRSPGPNF SLASQDVMV 
      VGEPSLMGG EFGDEDERL ITRLENTQY DAANSLDHD 
      NHGFHADSP MPGSNSWGA GVGDRGPGV GPSQGNTPS 
      NQDSDKKSP AVSQ
    CDS sequence
    • >TC000244 RA
      atgagacaa ggtcgcgcc ggccaacgg aaagcaaga 
      tgccttagc cgccgcgta gcgcccccc atcacgctg 
      ccagtcacc ctggaggac cccgccacg acctggaag 
      ggaccgccg cccggctcg gaaccgcag aacttcgct 
      accaacgcg gcaaactcg ttctccggg ccgccggcg 
      ggcttccaa gggccgtcg cctttccag ggcccgccg 
      gcgggggcc gggtcgccg gccggcgcg ccgccctac 
      caagggggc cccccgccg ggctcctcg acgccgcag 
      tacaccgcc tctccggcg ccgtcagga tcgtcgacg 
      ccggggccc gggcccccc caaagcgtc ggcaatgcg 
      tttccgccg cccccgaac agcgcggga cccccctac 
      aacgggccg gggcccgga cccttcggc tcgccgtcc 
      gccggagga ccgcccttc ggccggccc ggatcctcc 
      ggacctccg ttcgtaggg gggccagga cctggcggc 
      aacccgcac tttgcctcg cctggacaa cccttcgca 
      cccgggttc gggattccc ccaggatcg cccttcggg 
      cccgggccg ggacatccc atgtccggg cccatggaa 
      cccggacac ggcatcatg gccaggcaa atacccgga 
      cccattcca gtagaccaa ggatctctg cccgtccca 
      aggcggcat acaccttat ttcggtcaa cctgactac 
      cgaatatac gaactcaac aagaggcta cagcaaagg 
      acggaggaa agtgataat ctttggtgg gacgcgttt 
      gcgacggag ttttttgaa gacgacgcc tcattaacg 
      ctagctttt tgtctggaa gacggccct aaacgatac 
      acaatcgga aggacgtta attccgagg tactttagg 
      agtatattt gaaggtgga gtgacggaa ctgtactat 
      aatatgaag catccgaaa gagtccttc cacaacact 
      agtattacg ctagactgt gatcaatgc actatgatt 
      acgcatcat ggtaaaccc ttatttaca aaggtatgt 
      acagaaggt cgtttaatc ctagaattt acctttgat 
      gacctgatg aggataaag tcgtggcat ttcgcggtg 
      cgaacgcac cgcgaatta atcccccga tctgtggta 
      ggaatgcat tcgcagcaa gaccccagc atgttagat 
      caactatca aaaaacatc acgaggcaa ggaatcacg 
      aattccacc ctcaactac cttaggcta tgtgtaata 
      ctagaaccc atgcaggaa ttaatgtct aggcataaa 
      gcttacgca ttgagtccg agggattgt ttaaaaacc 
      acacttttc caaaagtgg caaaggatg gttgccccg 
      ccggaatca caacggccc gctaataaa cggagaaaa 
      cggaaagga tccaactcg ggaggggca cccaataat 
      gcaactcca acacctaac aagaagagg tcgccagga 
      ccaaacttt tcgttagca tcgcaggat gtgatggtg 
      gtgggcgag ccctcgctg atgggcggc gagttcggg 
      gacgaagac gagcgcttg ataacgcgt ctggagaac 
      acgcagtac gacgcggcc aactcgtta gaccatgac 
      aaccacggc ttccacgcg gactctccg atgccgggg 
      tcgaactcg tggggcgcc ggggttggc gaccggggt 
      cccggcgtg ggcccctcg cagggcaac accccctcg 
      aatcaggac tctgataaa aaatcgccc gctgtgagc 
      cagtga
    mRNA sequence
    • >TC000244 RA
      aaaatggcg ggaagttga gattcttca cagtattca 
      catctatgt gataaagaa gaatcgtgt aataaattt 
      agtttgtct caaggtccg caggtagat cggagggta 
      acctgactt actgtagta aaccttgaa tgatgtatg 
      tgtatatgc gacaaggaa cctgtcgac gactccaca 
      ctcgtctca tacgcaata actaaagaa aacaagtaa 
      aaacaccca cctgaagtg ttgaaatag aggcgaatt 
      ggacggtag tccatggag aacaggaga acatcttcg 
      agtttagat gcaaggtca cgtaattaa aatagcttc 
      tggcacttt tggcgacct tgaacccgg ccgaggtta 
      catgaatac tactttttt tccagatac aattactac 
      gcattgctt cggcttgtt taatgcgaa aatagcgat 
      aacaccaag cggatttat cagactgat tagtaaatt 
      ttacacaaa ctgatttgt gatttctat ttttaatca 
      gaaaagtta taagcggcc ttagcagtt tccggtttg 
      ttaccctga cccattcat aatcccaac acttataaa 
      acgatccat caagtagag attttttta tgactcccg 
      cagatttaa caacttcaa cgagatact ttatcgcag 
      aggatgtaa aatacatca actccagaa gactgtgtt 
      tgcaacata aatgttttt ctctgagaa aacggaatg 
      aaagagtta ccttgaacc caacacgag attttatgg 
      tccgtaatt tagaaaagt taaaaaatg ctttattgc 
      caaagattg gtgtggaat accaaacac acataaaag 
      gaaaaaaaa gagaaaaca tcagcaagg ataaaaagc 
      ttcgtaatt atcataagg ataaagcaa agaccctgc 
      tatcagggt taattttta caattttac tgggagatc 
      agtggaata aaatttcag tgtttcagt taaatgaaa 
      tgtctgaag tcttggttc tggtcagta gacgcgacc 
      gcatcgttt tgtaacggt gttaaaagt gtaaggagt 
      ccgtgaggt acgccactg gtgtcgcac gccggtcgc 
      cggtgggaa agtcggggc ccagtcaag ccgagcaaa 
      ggtagagga ggacgaggt aatgatgat gatgaggcg 
      agcaggagc aggagatcg gcaaagtgc aacgcatgg 
      aagcgtagt tgtcaattg tcaccgtca aaggggggc 
      accggcatc ccctctatt gttcatagc cggcccatg 
      tgcggcggt cgcactttt ctgctgatg ctttttatg 
      cggatgaac cgacagtgt aaagtcacg ctagtggaa 
      gcgaggcgg cggcggcac tccgtccta gtggcagta 
      attccatgt cgagccgat aagcggcta ccggctgct 
      ctgctccac ttacttcag acgtggcaa agcaaagca 
      aagctcacc ggagacacc agtgacgtc ctgacgcga 
      tcggtaacc gccttgcct ccgtcaccg acgcggtgt 
      gtagtaatg atagtcgtg atgttgttg aatgagcag 
      agggtgggg aagggctga ggggtggtg aactttatt 
      gatttttag ggtgacact gacaactac cgttgactg 
      agttcgcga tggaggact tgcaaacga aaaaaatca 
      tgcacactc agccggccc caaaaaact gaacgtgtc 
      cccggtgtc gcttgcttg cgctttttc ctgccactt 
      tggctcagt ttcgtcgag tttgtggcg agtggtttg 
      tgctgctgt tgagtgttt ttgaatttg aaaccggtg 
      gaatgtgtt gtcggtcaa acgcagcgc tgtcggcag 
      tgcacgcct cggccgcag ctcgcgtca accgtgtca 
      cgctgtaaa aatggcagc acttggtgg cttggatca 
      tttttgcac aaggcctaa gaagaaatt tagttgacc 
      ctgttgcca ttgctcccc gaccagaaa aggccagcg 
      gcaaagcca gcaaaggtg ggcgtttgt tgcttgcgt 
      tcgtttgcg tctcttgcc tcccggacc cgttatctc 
      accgtaaac atcaccaaa caatcgcca aaacacaca 
      ttttgcatc acaaatgca ctttttaca ccgggacac 
      taaaagaaa gcttcctaa cctaaaaaa ttatgacct 
      taatcgctg tcataactg tcaaatttt ttccgaatt 
      ttttacttg gaccatagc cgattgtaa tcggccgac 
      ggaattaac gtttttgtt tgttgggat tgcgttttt 
      tgtgggtat ttgtttgta aatttgcgt ttagcggcg 
      actttcaaa tgccggccg gcaagacag gtaactgca 
      taatagcat atcaacaat ggcggcagc caaacatgc 
      gctatggtt tgacttgaa cagtgttta cttgatgtg 
      tttcttgag aagttgtat ttagattcg gtgattcta 
      aaattaacg tgttttaaa tgaaagtag ataaaaatt 
      gagtgaatt cgtcgaaga tgttgtgca gtgatcgtt 
      tgttgatac ctgtggata gtttaagag tgtttggtt 
      cgcatcaat ttgtttgtt agagaaaag tccagtggt 
      tgtagttat gagtcgggt atcccgaaa atcgcgaaa 
      aatttcaaa aattcccac cgtgtgacg ctggcagca 
      tgagacaag gtcgcgccg gccaacgga aagcaagat 
      gccttagcc gccgcgtag cgcccccca tcacgctgc 
      cagtcaccc tggaggacc ccgccacga cctggaagg 
      gaccgccgc ccggctcgg aaccgcaga acttcgcta 
      ccaacgcgg caaactcgt tctccgggc cgccggcgg 
      gcttccaag ggccgtcgc ctttccagg gcccgccgg 
      cgggggccg ggtcgccgg ccggcgcgc cgccctacc 
      aagggggcc ccccgccgg gctcctcga cgccgcagt 
      acaccgcct ctccggcgc cgtcaggat cgtcgacgc 
      cggggcccg ggccccccc aaagcgtcg gcaatgcgt 
      ttccgccgc ccccgaaca gcgcgggac ccccctaca 
      acgggccgg ggcccggac ccttcggct cgccgtccg 
      ccggaggac cgcccttcg gccggcccg gatcctccg 
      gacctccgt tcgtagggg ggccaggac ctggcggca 
      acccgcact ttgcctcgc ctggacaac ccttcgcac 
      ccgggttcg ggattcccc caggatcgc ccttcgggc 
      ccgggccgg gacatccca tgtccgggc ccatggaac 
      ccggacacg gcatcatgg ccaggcaaa tacccggac 
      ccattccag tagaccaag gatctctgc ccgtcccaa 
      ggcggcata caccttatt tcggtcaac ctgactacc 
      gaatatacg aactcaaca agaggctac agcaaagga 
      cggaggaaa gtgataatc tttggtggg acgcgtttg 
      cgacggagt tttttgaag acgacgcct cattaacgc 
      tagcttttt gtctggaag acggcccta aacgataca 
      caatcggaa ggacgttaa ttccgaggt actttagga 
      gtatatttg aaggtggag tgacggaac tgtactata 
      atatgaagc atccgaaag agtccttcc acaacacta 
      gtattacgc tagactgtg atcaatgca ctatgatta 
      cgcatcatg gtaaaccct tatttacaa aggtatgta 
      cagaaggtc gtttaatcc tagaattta cctttgatg 
      acctgatga ggataaagt cgtggcatt tcgcggtgc 
      gaacgcacc gcgaattaa tcccccgat ctgtggtag 
      gaatgcatt cgcagcaag accccagca tgttagatc 
      aactatcaa aaaacatca cgaggcaag gaatcacga 
      attccaccc tcaactacc ttaggctat gtgtaatac 
      tagaaccca tgcaggaat taatgtcta ggcataaag 
      cttacgcat tgagtccga gggattgtt taaaaacca 
      cacttttcc aaaagtggc aaaggatgg ttgccccgc 
      cggaatcac aacggcccg ctaataaac ggagaaaac 
      ggaaaggat ccaactcgg gaggggcac ccaataatg 
      caactccaa cacctaaca agaagaggt cgccaggac 
      caaactttt cgttagcat cgcaggatg tgatggtgg 
      tgggcgagc cctcgctga tgggcggcg agttcgggg 
      acgaagacg agcgcttga taacgcgtc tggagaaca 
      cgcagtacg acgcggcca actcgttag accatgaca 
      accacggct tccacgcgg actctccga tgccggggt 
      cgaactcgt ggggcgccg gggttggcg accggggtc 
      ccggcgtgg gcccctcgc agggcaaca ccccctcga 
      atcaggact ctgataaaa aatcgcccg ctgtgagcc 
      agtgatgat agttcaatc gtaacgaac atgtagaat 
      ctctccgag tcagtattt cctgttgaa agtgcgatc 
      acacgatga tactagcca gtgacaagt tgtttttca 
      ttttttatt taattttat atgcaagtt tacaaagta 
      aaagaatta cttaagtgc aagatttta ttgggagtg 
      agatgaaaa ctgcgttta taatacaga gtggatcga 
      gtggagact ggaataatg cgttgtcgg ggttgtggt 
      ggcagcact gaaggtggt gacaacaca agctggata 
      aggttattg cacccgaaa ttgtgcaat cttaaaatg 
      gttcctttt ggaaccact caccatagg tacctctac 
      agagtgaat gtggttctc gaatgatgc agctcattt 
      gtacagtag atataaaac atcacactg tcttgtttg 
      tcttctgag taacacaga tatttcaag tggacgcct 
      cgggttaaa ttccgacag tgggggccg ccgtaaggc 
      caggtggtg gtgttttat ttattggtt gaatcacat 
      ttcctatat ttagctgta gggttaagt tcctagcga 
      tttttacga aatcaccga accttacgg ccgtcaaac 
      cgtccgaat ttcatccag aagcgtact ttacgagtg 
      gcactcttg atcggaggc gatctcgtt tattaggat 
      caattttgt atagtgaag ataaattta aattgtata 
      acatactta taaatatta tgtccattg agaatatgt 
      aatttacat ccaaatgtg atgtatttt ataggagcg 
      tctgagtga gtgggattt taagaaaac tgttctttt 
      aaacgaatg cctgcaatt tctgtatcg gtgtcagaa 
      tgcgagttt taattgtaa caagtaatt aaaaaatta 
      aaatttggg actgtagta ttttttact aagtctcat 
      ttccagctg tgtaagtgt atttttaca actcttttg 
      tcagtagtt gtgtgtgtc tttccttgt tccggttgt 
      ttggtttat ttccatttt ttcaatttg taatctcat 
      gtgtaaaga gactgtacc cagtacagc tcctactgc 
      aacctcaca atacaacac ttacaaata ttgtcagtt 
      ttatttatt caccgaatt gttgcgaaa atactacaa 
      atcctacaa caaacaacg ctgcgagtg aggtttaaa 
      aaccattca attttcctt tctttttct ccaaaaatt 
      gaattgttt ttagttgtt ccacgactt acgaaacaa 
      gcaatatta cgtagaaat ttgttttac cattgtttg 
      ggtgaattc aagttcgtt acacttttg tatagtcac 
      actgaaaat gtcattatt tgtaagaaa caataaaac 
      tgacataaa act
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY