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Gene information
General information
TC000230 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14431299..14432914
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000230
    iBeetle screen iB_02995

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000230 RA
      MLRGDVLIL FLVILITIT SSQKCPVCQ PRNFIENDQ 
      RCVCPQNEF CRDNKKCQK CDHNYTTWN DLIKLRKGE 
      IQTIFSNLI KRYEINVNE LKTLLNTFA EKLKYEEKA 
      LDLIGANSD KIQKENEII LKKIEKLNN GLNEIEEIL 
      AKLETPIPK DQSALDYLN EIKETIKKL VEILKSLNF 
      DLADRNIYS LVDEFSAIN RNVTMMLAN FPNVRKNSL 
      NLALSTEKD IDLLESIIA KYGNYPTNF SEELQKNSQ 
      WIEKDLAYL FNILNNYYT VSFDVNGLK NNATRVRSM 
      AKNASSRLD QLLQDHENF EKLVQKAKN ETNFYQELQ 
      NVKQRLESL KQKIGEFQQ LLKKIKAHE EVERELDDL 
      TKKIKEFNE KEKKLHKNK RVMEKLRQK LNELIQKYE 
      KLKDVKPVG KLIRETSEQ TKKTQEVES VIADHCKV
    CDS sequence
    • >TC000230 RA
      atgttgcgc ggagacgtc ttaattctt tttcttgtt 
      attctaatt accatcaca agctcacaa aaatgccca 
      gtttgtcag ccgagaaat ttcattgaa aacgaccaa 
      agatgtgtt tgtccacaa aacgagttt tgtagagac 
      aataaaaaa tgtcaaaag tgtgatcac aattacaca 
      acttggaat gacctcatc aagctacga aaaggcgag 
      attcaaaca attttcagt aatttaatc aaaaggtac 
      gaaataaat gtaaacgaa ctgaaaact ctattgaat 
      acatttgcc gagaaattg aaatatgaa gaaaaggcg 
      cttgacttg ataggtgct aacagtgac aaaattcaa 
      aaagagaac gaaataatt ttaaagaaa atcgagaaa 
      ttaaataat ggtttgaac gaaatcgaa gaaatttta 
      gcgaaactc gagactccc attcccaaa gaccaaagt 
      gctttggat tatttgaac gaaataaaa gaaacaata 
      aaaaaattg gttgaaata ttgaaaagt ctgaacttt 
      gatttagct gatcgcaac atttatagt cttgttgat 
      gaatttagt gctataaat cgtaatgtt acaatgatg 
      cttgcaaat tttccaaat gttcgcaaa aactcgcta 
      aatttggct ctttctacc gaaaaagat atcgacttg 
      ttggaatca ataatagcc aaatacggg aattaccca 
      acaaatttt tccgaagaa ttacaaaaa aatagtcaa 
      tggattgaa aaagattta gcttattta ttcaatatt 
      ttaaacaat tactacacg gtatctttt gatgtaaat 
      ggattgaaa aataacgca acacgggtt agatcaatg 
      gccaaaaac gcgtcctca cgactggat caactgcta 
      caagatcat gaaaatttt gagaaactt gttcaaaaa 
      gctaaaaat gaaactaat ttctaccaa gaattgcaa 
      aatgtgaaa caacgttta gaaagttta aaacaaaaa 
      attggcgag ttccaacaa ttattaaaa aaaataaaa 
      gcacacgaa gaagtggag agggaatta gatgaccta 
      actaaaaaa attaaagaa ttcaatgaa aaagagaag 
      aaattacat aaaaacaag cgcgtgatg gaaaaactt 
      cgccaaaaa ctgaatgaa ttaatacaa aaatatgag 
      aagcttaag gacgtgaaa cctgtgggg aaattaata 
      cgcgaaaca agcgaacag acgaagaag acacaggaa 
      gttgaatca gtaattgca gaccattgt aaagtctaa
    mRNA sequence
    • >TC000230 RA
      aacagagaa aaattcctt atctctcgt tgtatttga 
      taagaggta ttcattatg ttgcgcgga gacgtctta 
      attcttttt cttgttatt ctaattacc atcacaagc 
      tcacaaaaa tgcccagtt tgtcagccg agaaatttc 
      attgaaaac gaccaaaga tgtgtttgt ccacaaaac 
      gagttttgt agagacaat aaaaaatgt caaaagtgt 
      gatcacaat tacacaact tggaatgac ctcatcaag 
      ctacgaaaa ggcgagatt caaacaatt ttcagtaat 
      ttaatcaaa aggtacgaa ataaatgta aacgaactg 
      aaaactcta ttgaataca tttgccgag aaattgaaa 
      tatgaagaa aaggcgctt gacttgata ggtgctaac 
      agtgacaaa attcaaaaa gagaacgaa ataatttta 
      aagaaaatc gagaaatta aataatggt ttgaacgaa 
      atcgaagaa attttagcg aaactcgag actcccatt 
      cccaaagac caaagtgct ttggattat ttgaacgaa 
      ataaaagaa acaataaaa aaattggtt gaaatattg 
      aaaagtctg aactttgat ttagctgat cgcaacatt 
      tatagtctt gttgatgaa tttagtgct ataaatcgt 
      aatgttaca atgatgctt gcaaatttt ccaaatgtt 
      cgcaaaaac tcgctaaat ttggctctt tctaccgaa 
      aaagatatc gacttgttg gaatcaata atagccaaa 
      tacgggaat tacccaaca aatttttcc gaagaatta 
      caaaaaaat agtcaatgg attgaaaaa gatttagct 
      tatttattc aatatttta aacaattac tacacggta 
      tcttttgat gtaaatgga ttgaaaaat aacgcaaca 
      cgggttaga tcaatggcc aaaaacgcg tcctcacga 
      ctggatcaa ctgctacaa gatcatgaa aattttgag 
      aaacttgtt caaaaagct aaaaatgaa actaatttc 
      taccaagaa ttgcaaaat gtgaaacaa cgtttagaa 
      agtttaaaa caaaaaatt ggcgagttc caacaatta 
      ttaaaaaaa ataaaagca cacgaagaa gtggagagg 
      gaattagat gacctaact aaaaaaatt aaagaattc 
      aatgaaaaa gagaagaaa ttacataaa aacaagcgc 
      gtgatggaa aaacttcgc caaaaactg aatgaatta 
      atacaaaaa tatgagaag cttaaggac gtgaaacct 
      gtggggaaa ttaatacgc gaaacaagc gaacagacg 
      aagaagaca caggaagtt gaatcagta attgcagac 
      cattgtaaa gtctaaata aaattataa taaaaaatt 
      gtgtttttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY