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Gene information
General information
TC000177 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 15076486..15080189
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000177
    iBeetle screen iB_02989

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    abdomen irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment partially fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000177 RA
      MADSDAARS AIRVAENMK VPNGPRVVT INKTETGFG 
      FNVRGQVSE GGQLRSING ELYAPLQHV SAVLDRGAA 
      EQAGIRKGD RILEVNGVN VEGATHKQV VDLIKSGGD 
      VLTLTVISV TQQEAERLE PTDDNQVYY DYSEKRSLP 
      ISIPDYHYN ERGGERYVA FNIYMAGRH LCSRRYREF 
      SKLHVDLKK EFIGFTFPK LPGKWPFNL SEQQLDARR 
      RGLEQYLER VCAVRVIAE SDIMQEFLT DQDDDNNSS 
      PVDLKVLLP DREVVTVSL KKSANADEV YEAVVKKIG 
      MNKRVSKYF YLFEIVEYN FERKLQPTE YPHNLYIQN 
      YSTATSTCL CVRKWLFSL SKELTLMND TQATSYIFW 
      QSVDEVNRG YINAGERLY QLKALQDNT RASEYLKLA 
      RELPGYGEV VFPHCACDS RKDGHVIVA VGSGGIKLH 
      ACREDGTLE SQVVGLQWD CIRQWEVDD EGMAFCLKY 
      NRADKTPRW LKIFTPYHL YVMDCFDRI IEESKWLDT 
      GE
    CDS sequence
    • >TC000177 RA
      atggccgac tctgatgcg gcccgaagt gcaataaga 
      gtcgccgaa aacatgaaa gtgccaaat gggccccgt 
      gttgtcacc ataaataaa acagagact gggttcggt 
      tttaatgtc cgggggcaa gtgagcgaa gggggccaa 
      ctgcgaagt atcaatggg gagttgtac gccccctta 
      cagcatgtc agcgctgtg ttggacagg ggggctgcc 
      gaacaggcc gggatacgc aaaggcgac cggattcta 
      gaagtaaat ggtgtgaac gtggaagga gccacccac 
      aagcaagtg gtggacttg ataaaatcc ggcggtgat 
      gtgctcacc ctcaccgtc atatcagtg acacaacag 
      gaggcggag cgtttggaa ccgactgat gataatcaa 
      gtgtattat gattatagt gagaaacgt tcgttgccg 
      attagtatt cccgattat cattacaac gaacgaggg 
      ggtgaaagg tatgtggcg tttaatatt tacatggca 
      gggcggcat ttgtgctca cgacggtat cgagagttc 
      tctaagctg catgttgac ctcaagaag gagtttata 
      gggttcact tttccgaaa ttgccaggc aagtggccg 
      tttaattta agcgaacaa cagttggat gcacgtcga 
      agaggcttg gagcagtac ttggagagg gtttgcgct 
      gtgcgagtg attgccgag tctgatatt atgcaagag 
      ttcctcact gatcaagac gatgataat aatagcagt 
      ccagttgat cttaaagta ttacttccc gatcgtgaa 
      gttgtcaca gtgtcgtta aaaaaaagc gccaacgct 
      gacgaagtc tacgaagcc gttgtcaaa aaaatcggc 
      atgaataaa cgcgtttcg aaatatttt tacttattt 
      gaaattgtc gaatacaac ttcgaacgc aaactacaa 
      cccaccgaa tacccgcat aacctctac atccagaac 
      tacagtacg gcaacaagt acgtgtcta tgtgtccgt 
      aaatggctc ttttcccta tcgaaagaa ctgactctt 
      atgaatgac actcaagcc acttcgtac atcttttgg 
      cagtcggtc gatgaggtg aatcgaggt tatataaac 
      gccggcgag cgtttgtac caactcaaa gccctccaa 
      gacaacact cgcgcttct gaatatttg aaattagcg 
      cgcgaatta ccaggatat ggcgaggtg gtatttccc 
      cattgcgca tgcgattcg cggaaagac ggtcacgtg 
      attgtcgct gtggggagt gggggtata aaactacac 
      gcgtgtcga gaggatggg actttggaa agtcaagtg 
      gtggggctc caatgggat tgcataaga cagtgggag 
      gtggatgat gaagggatg gccttttgt ttgaaatac 
      aacagagcg gataagacg ccacgatgg ttgaagatt 
      ttcacacct tatcatttg tatgtcatg gattgcttc 
      gatcggatt attgaagag agcaagtgg ttggatacg 
      ggcgagtga
    mRNA sequence
    • >TC000177 RA
      aaaatatgg ccgactctg atgcggccc gaagtgcaa 
      taagagtcg ccgaaaaca tgaaagtgc caaatgggc 
      cccgtgttg tcaccataa ataaaacag agactgggt 
      tcggtttta atgtccggg ggcaagtga gcgaagggg 
      gccaactgc gaagtatca atggggagt tgtacgccc 
      ccttacagc atgtcagcg ctgtgttgg acagggggg 
      ctgccgaac aggccggga tacgcaaag gcgaccgga 
      ttctagaag taaatggtg tgaacgtgg aaggagcca 
      cccacaagc aagtggtgg acttgataa aatccggcg 
      gtgatgtgc tcaccctca ccgtcatat cagtgacac 
      aacaggagg cggagcgtt tggaaccga ctgatgata 
      atcaagtgt attatgatt atagtgaga aacgttcgt 
      tgccgatta gtattcccg attatcatt acaacgaac 
      gagggggtg aaaggtatg tggcgttta atatttaca 
      tggcagggc ggcatttgt gctcacgac ggtatcgag 
      agttctcta agctgcatg ttgacctca agaaggagt 
      ttatagggt tcacttttc cgaaattgc caggcaagt 
      ggccgttta atttaagcg aacaacagt tggatgcac 
      gtcgaagag gcttggagc agtacttgg agagggttt 
      gcgctgtgc gagtgattg ccgagtctg atattatgc 
      aagagttcc tcactgatc aagacgatg ataataata 
      gcagtccag ttgatctta aagtattac ttcccgatc 
      gtgaagttg tcacagtgt cgttaaaaa aaagcgcca 
      acgctgacg aagtctacg aagccgttg tcaaaaaaa 
      tcggcatga ataaacgcg tttcgaaat atttttact 
      tatttgaaa ttgtcgaat acaacttcg aacgcaaac 
      tacaaccca ccgaatacc cgcataacc tctacatcc 
      agaactaca gtacggcaa caagtacgt gtctatgtg 
      tccgtaaat ggctctttt ccctatcga aagaactga 
      ctcttatga atgacactc aagccactt cgtacatct 
      tttggcagt cggtcgatg aggtgaatc gaggttata 
      taaacgccg gcgagcgtt tgtaccaac tcaaagccc 
      tccaagaca acactcgcg cttctgaat atttgaaat 
      tagcgcgcg aattaccag gatatggcg aggtggtat 
      ttccccatt gcgcatgcg attcgcgga aagacggtc 
      acgtgattg tcgctgtgg ggagtgggg gtataaaac 
      tacacgcgt gtcgagagg atgggactt tggaaagtc 
      aagtggtgg ggctccaat gggattgca taagacagt 
      gggaggtgg atgatgaag ggatggcct tttgtttga 
      aatacaaca gagcggata agacgccac gatggttga 
      agattttca caccttatc atttgtatg tcatggatt 
      gcttcgatc ggattattg aagagagca agtggttgg 
      atacgggcg agtgacttg aagtgtttt aaatacata 
      ttatgttgt tgaactgta ttataattt atgctattt 
      atgtgatgt atatttttg gttataaaa tgattcgag 
      accataact tgttgaaat tattgtttt tgagagcac 
      aagttattt attaggaaa ttttttaaa aaattcatg 
      tgatagagt tattagtgt agatgaatt atctgtata 
      tataggttt agtgttaca tgtgatcac ttctgttgt 
      agtgggacc caattatct ttttgcact gccgttttt 
      ttttttttc gttagtagt ctctctttc taggcttca 
      aattctcct attttgagt ctcattcga caaaatgta 
      tatacataa gataatcgc gtagctcaa ttaatttaa 
      ttctagtgt ccaacagtc tagaatttt ttatattat 
      tctaggatc catcaagtg tttatctaa taaaatatt 
      tatttctgt tgatatcga ttccaaatt atattgtta 
      ctatatggc atcttaaaa tttggctgt tacgcacta 
      aattctaaa tatatac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY