General information | |||
TC000164 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 15179795..15183829 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC000164 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02987 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
ovary without detectable defects - Number of animals showing the phenotype: 1
larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
antenna shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC000164 RA
MNTGSTQDI VIEVLQRAS SQNPEVLKP AEAKLREWE TQPGFYTVL FNVLSNHSL DINVRWIAV LYIKNGIER YWRKNAPNA ILEAEKQNI KHGLILSFN EPVSQIAVQ RAVLISKIA RIDCPKEWP ELFPTLLQV IESPDSLVQ HRGFLTLHH VVKAIASKR LTGDRRFFQ EFSSNIYSF VLNLWNNFT EQFMNDVMK GAASQLVLT NLEKALLTL RILRKLTVF GFYKPHQNQ DCMKFLKVI FDRAKMALQ CRKHLRDKE LVEQCEKFI THLTKVLIS VLDVHPFSF IDFIQPSFE FTTYFLFTP DGINFLFER FIIQCFNLI KNILLCVEY KPPKILEMC KQEETLKAH EIKINFFQA NTLTEICRK LVSHYFILT QEELEMWDA DPETFAIDE SGDSWKYSL RPCMETVFV TIFHEFRDA LAPVLLEMI HETNCIVLP EDTQGILRK DAVYNAVNL AAYDLYDEV DFDSWFTNT LTHELKMKQ NNYRVIRRR VCALIGRWT GIKLSSELR PALYECVIN LLGPDEDMA VRLTASATL RYAIDDFEF SSDQFRDYL DPAFNLLFN LLKEANECE TKMHVLNVM TLMVERVGQ TIKPHTDAL IHYLPLLWT ESEDHNMLR CAIVSTLVQ LVKALGSVS SELNPFLLP IIQLGTDTN QSAIVYLLE DCLELWLTV LENSTTMSN ELLQLFNNM PTLLEYSTE NLRLCLLIV LVHTLLAPD LVMRTHGVQ VITVCDNLM ADMKNEGIV MLIRMLEIF IRSSPILGC ETVFPILPR IFQKIYEGE DYPLLMSMY LSILARVLL SSHDVFTRV LRTLAQVHN ETDENVLGK ILNVWLSKM PMVSQLEQR KLLGLALTN LLTTQSRPV FQRFATVMV NILECLNDM EDSLLLSDG RSPSDYDDV VPYETDHDQ RKKQLILSD PVHTIVLKD YLQSQMYEL RNQIGLPQY EQLLQTVDP DTISQLKGY ITL
- >TC000164 RA
atgaatacg ggatcaaca caagacatc gtaattgaa gttctgcaa agagcctcg agtcaaaat ccagaagtg ttaaaaccg gctgaagcc aaattacgg gaatgggaa actcaacct ggattttac acagtttta tttaatgtt ttatctaac cactcgctt gatataaac gtacggtgg attgcagtt ttgtatata aaaaatggt attgaacga tattggaga aaaaacgca ccaaacgct attttagag gcagaaaaa caaaacatt aaacacggt ttaatttta agttttaac gaacccgtt agtcagatt gcggtacaa agagcggtt ttaatttcg aaaattgca cgaattgat tgtccgaaa gagtggccc gaattattt ccgacttta ctacaagta attgaaagt cctgattct ctagtccaa catcgagga tttttaact cttcatcat gtagttaaa gccattgct tcgaaacgg ttaacaggc gatcgccgt tttttccaa gagttttcc agtaatatt tattcgttt gtgttgaat ttatggaat aatttcacc gaacaattt atgaatgat gtaatgaaa ggcgcagct tcgcaacta gttcttaca aatttggaa aaagcacta ttgactttg aggatttta cgcaaattg actgttttt ggtttttat aaaccgcat caaaaccag gattgtatg aagtttttg aaagtgatt tttgatcgc gctaaaatg gcactgcaa tgcaggaaa catttgagg gataaagaa ctggtggaa caatgcgaa aaattcatt acacactta acgaaagtt ttaatatcc gtcttggac gtacatcca ttttcattc attgatttc attcaacct tcgttcgaa tttactact tattttctt ttcactcct gatggaatt aatttcttg tttgaacgc ttcattatt cagtgtttt aatttaatt aaaaatatt ctcttgtgt gtggaatat aaaccgccg aaaattctc gaaatgtgt aaacaggag gaaacactt aaagcgcac gagatcaaa attaatttt tttcaagcc aatacttta accgaaatt tgccgaaaa ttggtctcg cattatttt attttgaca caagaagag ttggaaatg tgggatgct gatccagaa acgtttgcg attgatgaa agtggtgat agttggaag tatagtttg aggccttgt atggaaact gttttcgtg acaattttt cacgaattt cgggacgct ttagctccg gttttgctt gaaatgatt catgaaacc aactgtatt gttttaccc gaagacact caaggaata ttacgtaaa gacgcagtc tataatgca gttaacttg gccgcttat gatctttat gacgaggtc gatttcgat tcatggttc accaacaca ttaactcat gagcttaaa atgaaacag aacaattac cgtgtgatt cggcgaaga gtttgcgcc ttaatcggc cgttggact ggcataaaa ttatcatcg gaattacgt ccagcttta tacgaatgt gtgattaat ttattaggt cctgacgaa gacatggct gttcgctta acagcctct gcaacacta cgatacgct attgatgat tttgagttc agttccgat caatttcgt gattattta gacccagca tttaactta ttgtttaat ctgttgaaa gaagcgaac gaatgcgag acaaaaatg cacgttttg aacgtaatg accctaatg gtggagaga gtaggacag actattaaa ccacacact gatgcattg attcattat ttgccattg ttgtggacc gaatctgaa gatcataat atgttgaga tgtgcgatt gtttcgact ttggttcaa ttggtcaaa gctttgggg tcggttagt tcagaattg aacccgttt ttattaccg attatacaa ttaggcact gatacgaat caaagtgct attgtttat ctcttggag gattgtctc gagttgtgg ttgactgtg ttggaaaat tctacaact atgtcaaac gagcttttg caactattt aacaatatg ccaacttta ttggaatat tcaactgag aacttaaga ctttgccta ttgattgtg ttggttcac actttgttg gcgccagat cttgtcatg cgaacacac ggagttcaa gtgattaca gtttgtgat aatttaatg gccgatatg aaaaatgaa ggcattgta atgttgatc cgaatgttg gaaattttt attcgatcg tcaccgatt ctaggctgc gagactgtt ttccccatt ttaccacgt atattccaa aaaatctac gagggtgag gattatcct ctcttaatg tcaatgtac ctgtctata ctggcacgt gttttgctt tcctcgcat gatgttttt acacgtgtt ttgcgcacg ttggctcaa gttcataat gaaacggac gaaaatgtc cttggaaaa attttaaat gtttggtta tcgaaaatg ccaatggtg tctcaatta gaacagcgc aaacttttg ggtctagcg ttgacgaat ttattgacc actcaaagt cggccagtg tttcagaga tttgcaaca gttatggtc aatattttg gagtgtttg aacgatatg gaagattca cttttgttg tcggatggt cgtagtccg agtgattat gatgatgtt gtgccgtat gaaaccgat catgatcaa aggaagaaa caattgatt ttgtcggat ccagtgcat acgatagtt ttgaaagat tatttgcaa tcacaaatg tatgaactt aggaatcag attgggtta cctcagtat gaacaactg ctacaaaca gttgatcct gatactatt tcacaattg aaggggtat attacgttg tga
- >TC000164 RA
tgaaacatg tcatatgtg ttttgacaa ttgataatt ttatttaag aaaaaataa ttgtataat accggtcag ggggaaatg attataatt cacaatgaa tacgggatc aacacaaga catcgtaat tgaagttct gcaaagagc ctcgagtca aaatccaga agtgttaaa accggctga agccaaatt acgggaatg ggaaactca acctggatt ttacacagt tttatttaa tgttttatc taaccactc gcttgatat aaacgtacg gtggattgc agttttgta tataaaaaa tggtattga acgatattg gagaaaaaa cgcaccaaa cgctatttt agaggcaga aaaacaaaa cattaaaca cggtttaat tttaagttt taacgaacc cgttagtca gattgcggt acaaagagc ggttttaat ttcgaaaat tgcacgaat tgattgtcc gaaagagtg gcccgaatt atttccgac tttactaca agtaattga aagtcctga ttctctagt ccaacatcg aggattttt aactcttca tcatgtagt taaagccat tgcttcgaa acggttaac aggcgatcg ccgtttttt ccaagagtt ttccagtaa tatttattc gtttgtgtt gaatttatg gaataattt caccgaaca atttatgaa tgatgtaat gaaaggcgc agcttcgca actagttct tacaaattt ggaaaaagc actattgac tttgaggat tttacgcaa attgactgt ttttggttt ttataaacc gcatcaaaa ccaggattg tatgaagtt tttgaaagt gatttttga tcgcgctaa aatggcact gcaatgcag gaaacattt gagggataa agaactggt ggaacaatg cgaaaaatt cattacaca cttaacgaa agttttaat atccgtctt ggacgtaca tccattttc attcattga tttcattca accttcgtt cgaatttac tacttattt tcttttcac tcctgatgg aattaattt cttgtttga acgcttcat tattcagtg ttttaattt aattaaaaa tattctctt gtgtgtgga atataaacc gccgaaaat tctcgaaat gtgtaaaca ggaggaaac acttaaagc gcacgagat caaaattaa tttttttca agccaatac tttaaccga aatttgccg aaaattggt ctcgcatta ttttatttt gacacaaga agagttgga aatgtggga tgctgatcc agaaacgtt tgcgattga tgaaagtgg tgatagttg gaagtatag tttgaggcc ttgtatgga aactgtttt cgtgacaat ttttcacga atttcggga cgctttagc tccggtttt gcttgaaat gattcatga aaccaactg tattgtttt acccgaaga cactcaagg aatattacg taaagacgc agtctataa tgcagttaa cttggccgc ttatgatct ttatgacga ggtcgattt cgattcatg gttcaccaa cacattaac tcatgagct taaaatgaa acagaacaa ttaccgtgt gattcggcg aagagtttg cgccttaat cggccgttg gactggcat aaaattatc atcggaatt acgtccagc tttatacga atgtgtgat taatttatt aggtcctga cgaagacat ggctgttcg cttaacagc ctctgcaac actacgata cgctattga tgattttga gttcagttc cgatcaatt tcgtgatta tttagaccc agcatttaa cttattgtt taatctgtt gaaagaagc gaacgaatg cgagacaaa aatgcacgt tttgaacgt aatgaccct aatggtgga gagagtagg acagactat taaaccaca cactgatgc attgattca ttatttgcc attgttgtg gaccgaatc tgaagatca taatatgtt gagatgtgc gattgtttc gactttggt tcaattggt caaagcttt ggggtcggt tagttcaga attgaaccc gtttttatt accgattat acaattagg cactgatac gaatcaaag tgctattgt ttatctctt ggaggattg tctcgagtt gtggttgac tgtgttgga aaattctac aactatgtc aaacgagct tttgcaact atttaacaa tatgccaac tttattgga atattcaac tgagaactt aagactttg cctattgat tgtgttggt tcacacttt gttggcgcc agatcttgt catgcgaac acacggagt tcaagtgat tacagtttg tgataattt aatggccga tatgaaaaa tgaaggcat tgtaatgtt gatccgaat gttggaaat ttttattcg atcgtcacc gattctagg ctgcgagac tgttttccc cattttacc acgtatatt ccaaaaaat ctacgaggg tgaggatta tcctctctt aatgtcaat gtacctgtc tatactggc acgtgtttt gctttcctc gcatgatgt ttttacacg tgttttgcg cacgttggc tcaagttca taatgaaac ggacgaaaa tgtccttgg aaaaatttt aaatgtttg gttatcgaa aatgccaat ggtgtctca attagaaca gcgcaaact tttgggtct agcgttgac gaatttatt gaccactca aagtcggcc agtgtttca gagatttgc aacagttat ggtcaatat tttggagtg tttgaacga tatggaaga ttcactttt gttgtcgga tggtcgtag tccgagtga ttatgatga tgttgtgcc gtatgaaac cgatcatga tcaaaggaa gaaacaatt gattttgtc ggatccagt gcatacgat agttttgaa agattattt gcaatcaca aatgtatga acttaggaa tcagattgg gttacctca gtatgaaca actgctaca aacagttga tcctgatac tatttcaca attgaaggg gtatattac gttgtgaga atgacaaaa tgaaattct atctttagc aatatctta ttatttttg ttatatttt tgtaacata cctagttgt ataataatc gtttctttt agtccttat tcaaatgaa tttcattct aattattta ttgaataca tcgcttagt tattatgat tccgaaatt gatttgaaa cctacatat tgtatatga ttgtaccaa ataagatat aataaaaac tgttacaat ca
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
