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Gene information
General information
TC000164 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 15179795..15183829
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC000164
    iBeetle screen iB_02987

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
     antenna shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000164 RA
      MNTGSTQDI VIEVLQRAS SQNPEVLKP AEAKLREWE 
TQPGFYTVL FNVLSNHSL DINVRWIAV LYIKNGIER 
YWRKNAPNA ILEAEKQNI KHGLILSFN EPVSQIAVQ 
RAVLISKIA RIDCPKEWP ELFPTLLQV IESPDSLVQ 
HRGFLTLHH VVKAIASKR LTGDRRFFQ EFSSNIYSF 
VLNLWNNFT EQFMNDVMK GAASQLVLT NLEKALLTL 
RILRKLTVF GFYKPHQNQ DCMKFLKVI FDRAKMALQ 
CRKHLRDKE LVEQCEKFI THLTKVLIS VLDVHPFSF 
IDFIQPSFE FTTYFLFTP DGINFLFER FIIQCFNLI 
KNILLCVEY KPPKILEMC KQEETLKAH EIKINFFQA 
NTLTEICRK LVSHYFILT QEELEMWDA DPETFAIDE 
SGDSWKYSL RPCMETVFV TIFHEFRDA LAPVLLEMI 
HETNCIVLP EDTQGILRK DAVYNAVNL AAYDLYDEV 
DFDSWFTNT LTHELKMKQ NNYRVIRRR VCALIGRWT 
GIKLSSELR PALYECVIN LLGPDEDMA VRLTASATL 
RYAIDDFEF SSDQFRDYL DPAFNLLFN LLKEANECE 
TKMHVLNVM TLMVERVGQ TIKPHTDAL IHYLPLLWT 
ESEDHNMLR CAIVSTLVQ LVKALGSVS SELNPFLLP 
IIQLGTDTN QSAIVYLLE DCLELWLTV LENSTTMSN 
ELLQLFNNM PTLLEYSTE NLRLCLLIV LVHTLLAPD 
LVMRTHGVQ VITVCDNLM ADMKNEGIV MLIRMLEIF 
IRSSPILGC ETVFPILPR IFQKIYEGE DYPLLMSMY 
LSILARVLL SSHDVFTRV LRTLAQVHN ETDENVLGK 
ILNVWLSKM PMVSQLEQR KLLGLALTN LLTTQSRPV 
FQRFATVMV NILECLNDM EDSLLLSDG RSPSDYDDV 
VPYETDHDQ RKKQLILSD PVHTIVLKD YLQSQMYEL 
RNQIGLPQY EQLLQTVDP DTISQLKGY ITL
    CDS sequence
    • >TC000164 RA
      atgaatacg ggatcaaca caagacatc gtaattgaa 
gttctgcaa agagcctcg agtcaaaat ccagaagtg 
ttaaaaccg gctgaagcc aaattacgg gaatgggaa 
actcaacct ggattttac acagtttta tttaatgtt 
ttatctaac cactcgctt gatataaac gtacggtgg 
attgcagtt ttgtatata aaaaatggt attgaacga 
tattggaga aaaaacgca ccaaacgct attttagag 
gcagaaaaa caaaacatt aaacacggt ttaatttta 
agttttaac gaacccgtt agtcagatt gcggtacaa 
agagcggtt ttaatttcg aaaattgca cgaattgat 
tgtccgaaa gagtggccc gaattattt ccgacttta 
ctacaagta attgaaagt cctgattct ctagtccaa 
catcgagga tttttaact cttcatcat gtagttaaa 
gccattgct tcgaaacgg ttaacaggc gatcgccgt 
tttttccaa gagttttcc agtaatatt tattcgttt 
gtgttgaat ttatggaat aatttcacc gaacaattt 
atgaatgat gtaatgaaa ggcgcagct tcgcaacta 
gttcttaca aatttggaa aaagcacta ttgactttg 
aggatttta cgcaaattg actgttttt ggtttttat 
aaaccgcat caaaaccag gattgtatg aagtttttg 
aaagtgatt tttgatcgc gctaaaatg gcactgcaa 
tgcaggaaa catttgagg gataaagaa ctggtggaa 
caatgcgaa aaattcatt acacactta acgaaagtt 
ttaatatcc gtcttggac gtacatcca ttttcattc 
attgatttc attcaacct tcgttcgaa tttactact 
tattttctt ttcactcct gatggaatt aatttcttg 
tttgaacgc ttcattatt cagtgtttt aatttaatt 
aaaaatatt ctcttgtgt gtggaatat aaaccgccg 
aaaattctc gaaatgtgt aaacaggag gaaacactt 
aaagcgcac gagatcaaa attaatttt tttcaagcc 
aatacttta accgaaatt tgccgaaaa ttggtctcg 
cattatttt attttgaca caagaagag ttggaaatg 
tgggatgct gatccagaa acgtttgcg attgatgaa 
agtggtgat agttggaag tatagtttg aggccttgt 
atggaaact gttttcgtg acaattttt cacgaattt 
cgggacgct ttagctccg gttttgctt gaaatgatt 
catgaaacc aactgtatt gttttaccc gaagacact 
caaggaata ttacgtaaa gacgcagtc tataatgca 
gttaacttg gccgcttat gatctttat gacgaggtc 
gatttcgat tcatggttc accaacaca ttaactcat 
gagcttaaa atgaaacag aacaattac cgtgtgatt 
cggcgaaga gtttgcgcc ttaatcggc cgttggact 
ggcataaaa ttatcatcg gaattacgt ccagcttta 
tacgaatgt gtgattaat ttattaggt cctgacgaa 
gacatggct gttcgctta acagcctct gcaacacta 
cgatacgct attgatgat tttgagttc agttccgat 
caatttcgt gattattta gacccagca tttaactta 
ttgtttaat ctgttgaaa gaagcgaac gaatgcgag 
acaaaaatg cacgttttg aacgtaatg accctaatg 
gtggagaga gtaggacag actattaaa ccacacact 
gatgcattg attcattat ttgccattg ttgtggacc 
gaatctgaa gatcataat atgttgaga tgtgcgatt 
gtttcgact ttggttcaa ttggtcaaa gctttgggg 
tcggttagt tcagaattg aacccgttt ttattaccg 
attatacaa ttaggcact gatacgaat caaagtgct 
attgtttat ctcttggag gattgtctc gagttgtgg 
ttgactgtg ttggaaaat tctacaact atgtcaaac 
gagcttttg caactattt aacaatatg ccaacttta 
ttggaatat tcaactgag aacttaaga ctttgccta 
ttgattgtg ttggttcac actttgttg gcgccagat 
cttgtcatg cgaacacac ggagttcaa gtgattaca 
gtttgtgat aatttaatg gccgatatg aaaaatgaa 
ggcattgta atgttgatc cgaatgttg gaaattttt 
attcgatcg tcaccgatt ctaggctgc gagactgtt 
ttccccatt ttaccacgt atattccaa aaaatctac 
gagggtgag gattatcct ctcttaatg tcaatgtac 
ctgtctata ctggcacgt gttttgctt tcctcgcat 
gatgttttt acacgtgtt ttgcgcacg ttggctcaa 
gttcataat gaaacggac gaaaatgtc cttggaaaa 
attttaaat gtttggtta tcgaaaatg ccaatggtg 
tctcaatta gaacagcgc aaacttttg ggtctagcg 
ttgacgaat ttattgacc actcaaagt cggccagtg 
tttcagaga tttgcaaca gttatggtc aatattttg 
gagtgtttg aacgatatg gaagattca cttttgttg 
tcggatggt cgtagtccg agtgattat gatgatgtt 
gtgccgtat gaaaccgat catgatcaa aggaagaaa 
caattgatt ttgtcggat ccagtgcat acgatagtt 
ttgaaagat tatttgcaa tcacaaatg tatgaactt 
aggaatcag attgggtta cctcagtat gaacaactg 
ctacaaaca gttgatcct gatactatt tcacaattg 
aaggggtat attacgttg tga
    mRNA sequence
    • >TC000164 RA
      tgaaacatg tcatatgtg ttttgacaa ttgataatt 
ttatttaag aaaaaataa ttgtataat accggtcag 
ggggaaatg attataatt cacaatgaa tacgggatc 
aacacaaga catcgtaat tgaagttct gcaaagagc 
ctcgagtca aaatccaga agtgttaaa accggctga 
agccaaatt acgggaatg ggaaactca acctggatt 
ttacacagt tttatttaa tgttttatc taaccactc 
gcttgatat aaacgtacg gtggattgc agttttgta 
tataaaaaa tggtattga acgatattg gagaaaaaa 
cgcaccaaa cgctatttt agaggcaga aaaacaaaa 
cattaaaca cggtttaat tttaagttt taacgaacc 
cgttagtca gattgcggt acaaagagc ggttttaat 
ttcgaaaat tgcacgaat tgattgtcc gaaagagtg 
gcccgaatt atttccgac tttactaca agtaattga 
aagtcctga ttctctagt ccaacatcg aggattttt 
aactcttca tcatgtagt taaagccat tgcttcgaa 
acggttaac aggcgatcg ccgtttttt ccaagagtt 
ttccagtaa tatttattc gtttgtgtt gaatttatg 
gaataattt caccgaaca atttatgaa tgatgtaat 
gaaaggcgc agcttcgca actagttct tacaaattt 
ggaaaaagc actattgac tttgaggat tttacgcaa 
attgactgt ttttggttt ttataaacc gcatcaaaa 
ccaggattg tatgaagtt tttgaaagt gatttttga 
tcgcgctaa aatggcact gcaatgcag gaaacattt 
gagggataa agaactggt ggaacaatg cgaaaaatt 
cattacaca cttaacgaa agttttaat atccgtctt 
ggacgtaca tccattttc attcattga tttcattca 
accttcgtt cgaatttac tacttattt tcttttcac 
tcctgatgg aattaattt cttgtttga acgcttcat 
tattcagtg ttttaattt aattaaaaa tattctctt 
gtgtgtgga atataaacc gccgaaaat tctcgaaat 
gtgtaaaca ggaggaaac acttaaagc gcacgagat 
caaaattaa tttttttca agccaatac tttaaccga 
aatttgccg aaaattggt ctcgcatta ttttatttt 
gacacaaga agagttgga aatgtggga tgctgatcc 
agaaacgtt tgcgattga tgaaagtgg tgatagttg 
gaagtatag tttgaggcc ttgtatgga aactgtttt 
cgtgacaat ttttcacga atttcggga cgctttagc 
tccggtttt gcttgaaat gattcatga aaccaactg 
tattgtttt acccgaaga cactcaagg aatattacg 
taaagacgc agtctataa tgcagttaa cttggccgc 
ttatgatct ttatgacga ggtcgattt cgattcatg 
gttcaccaa cacattaac tcatgagct taaaatgaa 
acagaacaa ttaccgtgt gattcggcg aagagtttg 
cgccttaat cggccgttg gactggcat aaaattatc 
atcggaatt acgtccagc tttatacga atgtgtgat 
taatttatt aggtcctga cgaagacat ggctgttcg 
cttaacagc ctctgcaac actacgata cgctattga 
tgattttga gttcagttc cgatcaatt tcgtgatta 
tttagaccc agcatttaa cttattgtt taatctgtt 
gaaagaagc gaacgaatg cgagacaaa aatgcacgt 
tttgaacgt aatgaccct aatggtgga gagagtagg 
acagactat taaaccaca cactgatgc attgattca 
ttatttgcc attgttgtg gaccgaatc tgaagatca 
taatatgtt gagatgtgc gattgtttc gactttggt 
tcaattggt caaagcttt ggggtcggt tagttcaga 
attgaaccc gtttttatt accgattat acaattagg 
cactgatac gaatcaaag tgctattgt ttatctctt 
ggaggattg tctcgagtt gtggttgac tgtgttgga 
aaattctac aactatgtc aaacgagct tttgcaact 
atttaacaa tatgccaac tttattgga atattcaac 
tgagaactt aagactttg cctattgat tgtgttggt 
tcacacttt gttggcgcc agatcttgt catgcgaac 
acacggagt tcaagtgat tacagtttg tgataattt 
aatggccga tatgaaaaa tgaaggcat tgtaatgtt 
gatccgaat gttggaaat ttttattcg atcgtcacc 
gattctagg ctgcgagac tgttttccc cattttacc 
acgtatatt ccaaaaaat ctacgaggg tgaggatta 
tcctctctt aatgtcaat gtacctgtc tatactggc 
acgtgtttt gctttcctc gcatgatgt ttttacacg 
tgttttgcg cacgttggc tcaagttca taatgaaac 
ggacgaaaa tgtccttgg aaaaatttt aaatgtttg 
gttatcgaa aatgccaat ggtgtctca attagaaca 
gcgcaaact tttgggtct agcgttgac gaatttatt 
gaccactca aagtcggcc agtgtttca gagatttgc 
aacagttat ggtcaatat tttggagtg tttgaacga 
tatggaaga ttcactttt gttgtcgga tggtcgtag 
tccgagtga ttatgatga tgttgtgcc gtatgaaac 
cgatcatga tcaaaggaa gaaacaatt gattttgtc 
ggatccagt gcatacgat agttttgaa agattattt 
gcaatcaca aatgtatga acttaggaa tcagattgg 
gttacctca gtatgaaca actgctaca aacagttga 
tcctgatac tatttcaca attgaaggg gtatattac 
gttgtgaga atgacaaaa tgaaattct atctttagc 
aatatctta ttatttttg ttatatttt tgtaacata 
cctagttgt ataataatc gtttctttt agtccttat 
tcaaatgaa tttcattct aattattta ttgaataca 
tcgcttagt tattatgat tccgaaatt gatttgaaa 
cctacatat tgtatatga ttgtaccaa ataagatat 
aataaaaac tgttacaat ca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY