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Gene information
General information
TC000150 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 41865..143392
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000150
    iBeetle screen iB_02985
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000150 RA
      MDLPTTVQV YFSVGQRHE AALFRSDSP AEQIKDLFR 
      SASEAGPRD ILKLYNTSG QLLNISGDL PPNTPDSRY 
      SLQVVAANG FAMLQESTG NSLKALEAR VSAIERQLR 
      SELPLPPAV EELQRQVEE FREKLETTE SLSWLALSR 
      FFKRSRFHA NIIRFSGFY SICNILGFY KQLPEPISS 
      EECRRLQYR RKSDSVKKR VKQNFANIC EAQVSDAIR 
      QWLRTPTFD ARPWEDEEL LLLLQQMYL DYDLCSKFA 
      IDINTLRNF LYEAYKNYN DVPFHNFRH CFCVAQMMY 
      AISWSVDLP SKVGDLEVL ILLTSCICH DLDHPGYNN 
      IYQINAKTE LAIRYNDIS PLENHHCSV AFRILENED 
      CNIFKSFKS EDFKTVREG IIRCILATD MARHNEILT 
      NFREITPNF DYNDKAHVN LLCMVLIKV SDISNEARP 
      MDVAEPWLD RLLQEFFKQ SDAEKLEGL PVTPFMDRE 
      KITKPSSQC SFIGFVLLP LFEALGDLL TELQDLIVQ 
      PVREALEYY RRLNEATRE ERLHRKSIV SEMTDQHTP 
      STQSPDSAV TVPKSLSNA SVKLKKSLS FQQNRSRSR 
      STDEDTEQS FGSGNMIGE EQSLEDVVE DPESGESET 
      ATEVEVSEK ALKFKISTE SSVGTGRKS YPGSRKGSR 
      ERVQHFNSS ELARMIQKG KLRSAGFSF DQHCMIGNK 
      KLSFDTPEF LDEYALTLK KRLDDQSDS EEEKQCVVS 
      KETLNLNKR NSEHLVKIP SNLEEKRSI LKCSDNKSF 
      TNADKLRMN NVKSSLEDD EKLIVNNRK NSQSRVNNT 
      SDSVNSIDK DEVRKVQAA AGSPRNSRS IFSRFRQFT 
      DRFSLSVDK DSKVKHPKN NNSTRNPFP HKKKKLETP 
      CCRSLEEVG RASTLPKTK SKKAWKFLV LGKEKDKWA 
      SDAAVDKLA VVETDNQSL PSTSQLQSN CASPKTHLE 
      KLEIVSLKE FKVEETVEK TGVEKGEEG GVI
    • >TC000150 RB
      MRKQCVTTN EDNYLNLFR SASEAGPRD ILKLYNTSG 
      QLLNISGDL PPNTPDSRY SLQVVAANG FAMLQESTG 
      NSLKALEAR VSAIERQLR SELPLPPAV EELQRQVEE 
      FREKLETTE SLSWLALSR FFKRSRFHA NIIRFSGFY 
      SICNILGFY KQLPEPISS EECRRLQYR RKSDSVKKR 
      VKQNFANIC EAQVSDAIR QWLRTPTFD ARPWEDEEL 
      LLLLQQMYL DYDLCSKFA IDINTLRNF LYEAYKNYN 
      DVPFHNFRH CFCVAQMMY AISWSVDLP SKVGDLEVL 
      ILLTSCICH DLDHPGYNN IYQINAKTE LAIRYNDIS 
      PLENHHCSV AFRILENED CNIFKSFKS EDFKTVREG 
      IIRCILATD MARHNEILT NFREITPNF DYNDKAHVN 
      LLCMVLIKV SDISNEARP MDVAEPWLD RLLQEFFKQ 
      SDAEKLEGL PVTPFMDRE KITKPSSQC SFIGFVLLP 
      LFEALGDLL TELQDLIVQ PVREALEYY RRLNEATRE 
      ERLHRKSIV SEMTDQHTP STQSPDSAV TVPKSLSNA 
      SVKLKKSLS FQQNRSRSR STDEDTEQS FGSGNMIGE 
      EQSLEDVVE DPESGESET ATEVEVSEK ALKFKISTE 
      SSVGTGRKS YPGSRKGSR ERVQHFNSS ELARMIQKG 
      KLRSAGFSF DQHCMIGNK KLSFDTPEF LDEYALTLK 
      KRLDDQSDS EEEKQCVVS KETLNLNKR NSEHLVKIP 
      SNLEEKRSI LKCSDNKSF TNADKLRMN NVKSSLEDD 
      EKLIVNNRK NSQSRVNNT SDSVNSIDK DEVRKVQAA 
      AGSPRNSRS IFSRFRQFT DRFSLSVDK DSKVKHPKN 
      NNSTRNPFP HKKKKLETP CCRSLEEVG RASTLPKTK 
      SKKAWKFLV LGKEKDKWA SDAAVDKLA VVETDNQSL 
      PSTSQLQSN CASPKTHLE KLEIVSLKE FKVEETVEK 
      TGVEKGEEG GVI
    CDS sequence
    • >TC000150 RA
      atggacctc cccacgacg gtgcaggtg tatttcagc 
      gtggggcag cgccacgag gcagcgctt ttccgctcc 
      gactcgccc gcggaacag attaaagat ttattcaga 
      tctgcctcc gaggctggt ccccgggac attctgaaa 
      ctgtacaac acctccggt caattgttg aacatcagc 
      ggagatttg cctcccaac acaccagac agcaggtac 
      tctctgcaa gtggtggcc gccaacggc ttcgctatg 
      ctccaggaa tcgaccgga aacagccta aaagctctg 
      gaagctcga gtatccgcc atcgaaaga cagctacga 
      tccgaactg ccgctgcct cccgccgtc gaagagctc 
      cagagacaa gtggaggag ttccgggag aagcttgag 
      actacggag agtctgagc tggctcgct ttgtcccgt 
      tttttcaag cgctcccgc tttcatgca aatatcata 
      cgttttagt ggcttttat tcgatttgt aatatttta 
      ggattctac aagcagctt ccggaaccc atctcgagt 
      gaagagtgc cgccgtttg cagtaccgg cgaaaaagc 
      gactcggtg aaaaaacga gtgaagcaa aacttcgcc 
      aacatttgt gaagctcaa gtttcggat gcgattcga 
      cagtggctg aggacaccg acgttcgac gccaggccc 
      tgggaggac gaagaattg cttctcctg cttcaacaa 
      atgtatctc gattatgat ctctgttca aagtttgct 
      attgacata aatacgctt agaaatttc ctctacgaa 
      gcctacaag aattataat gacgtgccc tttcacaat 
      tttagacac tgcttctgc gtcgcacaa atgatgtac 
      gccatctct tggagcgtg gatttgccc tcgaaagtc 
      ggcgatttg gaagttttg attttgcta acatcgtgc 
      atctgtcac gatctggac catcccggt tacaacaac 
      atttaccaa atcaacgca aagaccgaa ttagcgatc 
      cgttataac gacatatcg ccgttagag aatcaccac 
      tgtagcgta gcattcagg atactcgaa aacgaagac 
      tgcaacata ttcaagtct ttcaaaagc gaggatttc 
      aaaacagtg cgcgagggc atcatcagg tgcatatta 
      gctacagac atggctcga cataatgaa atcctcacc 
      aacttcagg gagattacg cccaatttt gactataac 
      gacaaagct cacgttaat ttgctttgt atggtcctt 
      atcaaagtg tcggatata tcgaacgaa gcgcggccg 
      atggacgtg gcggaacct tggctcgat agactcctg 
      caagaattc ttcaaacaa agcgacgcc gagaagctg 
      gaagggtta cctgtcacc ccgttcatg gacagggag 
      aaaataacg aaaccctcg tctcagtgt tcatttatc 
      ggcttcgtc ctcctccca ttgttcgaa gcgttggga 
      gacttactt actgagtta caagatctc attgttcaa 
      ccggttcgc gaagcgctg gaatattat aggagatta 
      aacgaagcg acgagagag gagcgactt cacaggaaa 
      agtatagtg tctgagatg acggaccag cacacccca 
      tccacacaa agccccgat agcgccgtt accgttccc 
      aaatcgctt tcgaatgcc agtgttaag ctgaaaaag 
      agcctcagc ttccagcaa aataggtcg aggtcccga 
      tccaccgac gaagacacc gaacagagc ttcggcagc 
      ggcaacatg atcggcgag gagcaaagc ctcgaagac 
      gtggtcgaa gaccccgaa agcggcgaa tcggaaacc 
      gccaccgaa gtcgaagtc tcagaaaag gccctcaag 
      ttcaaaata tcgaccgaa agtagcgtg ggaaccggt 
      cgcaaaagc taccccggc tccaggaaa ggcagccgg 
      gaacgcgtc caacacttc aacagctct gaactcgct 
      cgaatgatc cagaagggc aaactgcgc tccgccggc 
      ttcagcttc gaccaacac tgcatgata ggcaacaag 
      aagttatcc ttcgacact ccggagttt ttggacgag 
      tacgcttta accttgaag aagcggctg gacgaccaa 
      tcggacagc gaggaggag aagcaatgc gtggtctcg 
      aaagagacc ctcaacttg aacaagcgc aactcggag 
      catttggtc aagatcccg tcgaacctg gaggagaaa 
      cgcagcatt ctaaagtgt tctgacaat aaatctttc 
      acgaacgcg gacaaactc cggatgaac aacgtgaag 
      tcgtccctc gaagacgac gagaaactg atcgtcaac 
      aaccggaag aacagtcag tcgcgagtg aacaatacc 
      agtgatagc gttaacagt attgacaag gacgaagtg 
      cggaaagtg caagcggcg gccgggagt ccgcggaac 
      agccgttcg attttctcc cgctttcgg cagttcacc 
      gatcggttc agtctttcg gtggacaaa gactcgaag 
      gtgaagcac ccgaagaac aacaacagc acgcgaaac 
      cccttcccg cacaagaag aagaagctg gagacgccg 
      tgctgccgc agcttggag gaagttggc cgtgcgagc 
      acactcccg aagaccaag agcaagaaa gcgtggaag 
      ttcctggtg ctgggcaag gagaaggac aagtgggcg 
      tctgacgca gctgtggat aagctcgct gtggtcgaa 
      accgacaat caatctcta ccgtctacc tcacagctg 
      cagagtaat tgcgcgagt cccaagacg cacttggag 
      aagttggaa attgtgagc ttgaaggag ttcaaggtg 
      gaggaaacg gtggaaaaa acaggggtt gaaaagggt 
      gaagagggt ggagtgatt tga
    • >TC000150 RB
      atgcgtaaa caatgtgta acgacaaat gaagataat 
      tatttaaat ttattcaga tctgcctcc gaggctggt 
      ccccgggac attctgaaa ctgtacaac acctccggt 
      caattgttg aacatcagc ggagatttg cctcccaac 
      acaccagac agcaggtac tctctgcaa gtggtggcc 
      gccaacggc ttcgctatg ctccaggaa tcgaccgga 
      aacagccta aaagctctg gaagctcga gtatccgcc 
      atcgaaaga cagctacga tccgaactg ccgctgcct 
      cccgccgtc gaagagctc cagagacaa gtggaggag 
      ttccgggag aagcttgag actacggag agtctgagc 
      tggctcgct ttgtcccgt tttttcaag cgctcccgc 
      tttcatgca aatatcata cgttttagt ggcttttat 
      tcgatttgt aatatttta ggattctac aagcagctt 
      ccggaaccc atctcgagt gaagagtgc cgccgtttg 
      cagtaccgg cgaaaaagc gactcggtg aaaaaacga 
      gtgaagcaa aacttcgcc aacatttgt gaagctcaa 
      gtttcggat gcgattcga cagtggctg aggacaccg 
      acgttcgac gccaggccc tgggaggac gaagaattg 
      cttctcctg cttcaacaa atgtatctc gattatgat 
      ctctgttca aagtttgct attgacata aatacgctt 
      agaaatttc ctctacgaa gcctacaag aattataat 
      gacgtgccc tttcacaat tttagacac tgcttctgc 
      gtcgcacaa atgatgtac gccatctct tggagcgtg 
      gatttgccc tcgaaagtc ggcgatttg gaagttttg 
      attttgcta acatcgtgc atctgtcac gatctggac 
      catcccggt tacaacaac atttaccaa atcaacgca 
      aagaccgaa ttagcgatc cgttataac gacatatcg 
      ccgttagag aatcaccac tgtagcgta gcattcagg 
      atactcgaa aacgaagac tgcaacata ttcaagtct 
      ttcaaaagc gaggatttc aaaacagtg cgcgagggc 
      atcatcagg tgcatatta gctacagac atggctcga 
      cataatgaa atcctcacc aacttcagg gagattacg 
      cccaatttt gactataac gacaaagct cacgttaat 
      ttgctttgt atggtcctt atcaaagtg tcggatata 
      tcgaacgaa gcgcggccg atggacgtg gcggaacct 
      tggctcgat agactcctg caagaattc ttcaaacaa 
      agcgacgcc gagaagctg gaagggtta cctgtcacc 
      ccgttcatg gacagggag aaaataacg aaaccctcg 
      tctcagtgt tcatttatc ggcttcgtc ctcctccca 
      ttgttcgaa gcgttggga gacttactt actgagtta 
      caagatctc attgttcaa ccggttcgc gaagcgctg 
      gaatattat aggagatta aacgaagcg acgagagag 
      gagcgactt cacaggaaa agtatagtg tctgagatg 
      acggaccag cacacccca tccacacaa agccccgat 
      agcgccgtt accgttccc aaatcgctt tcgaatgcc 
      agtgttaag ctgaaaaag agcctcagc ttccagcaa 
      aataggtcg aggtcccga tccaccgac gaagacacc 
      gaacagagc ttcggcagc ggcaacatg atcggcgag 
      gagcaaagc ctcgaagac gtggtcgaa gaccccgaa 
      agcggcgaa tcggaaacc gccaccgaa gtcgaagtc 
      tcagaaaag gccctcaag ttcaaaata tcgaccgaa 
      agtagcgtg ggaaccggt cgcaaaagc taccccggc 
      tccaggaaa ggcagccgg gaacgcgtc caacacttc 
      aacagctct gaactcgct cgaatgatc cagaagggc 
      aaactgcgc tccgccggc ttcagcttc gaccaacac 
      tgcatgata ggcaacaag aagttatcc ttcgacact 
      ccggagttt ttggacgag tacgcttta accttgaag 
      aagcggctg gacgaccaa tcggacagc gaggaggag 
      aagcaatgc gtggtctcg aaagagacc ctcaacttg 
      aacaagcgc aactcggag catttggtc aagatcccg 
      tcgaacctg gaggagaaa cgcagcatt ctaaagtgt 
      tctgacaat aaatctttc acgaacgcg gacaaactc 
      cggatgaac aacgtgaag tcgtccctc gaagacgac 
      gagaaactg atcgtcaac aaccggaag aacagtcag 
      tcgcgagtg aacaatacc agtgatagc gttaacagt 
      attgacaag gacgaagtg cggaaagtg caagcggcg 
      gccgggagt ccgcggaac agccgttcg attttctcc 
      cgctttcgg cagttcacc gatcggttc agtctttcg 
      gtggacaaa gactcgaag gtgaagcac ccgaagaac 
      aacaacagc acgcgaaac cccttcccg cacaagaag 
      aagaagctg gagacgccg tgctgccgc agcttggag 
      gaagttggc cgtgcgagc acactcccg aagaccaag 
      agcaagaaa gcgtggaag ttcctggtg ctgggcaag 
      gagaaggac aagtgggcg tctgacgca gctgtggat 
      aagctcgct gtggtcgaa accgacaat caatctcta 
      ccgtctacc tcacagctg cagagtaat tgcgcgagt 
      cccaagacg cacttggag aagttggaa attgtgagc 
      ttgaaggag ttcaaggtg gaggaaacg gtggaaaaa 
      acaggggtt gaaaagggt gaagagggt ggagtgatt 
      tga
    mRNA sequence
    • >TC000150 RA
      tagatgtta ttggactgt tttctgttt cagatcgaa 
      gcgtaggat ggcagcagc gagtgggcg cgccgtcaa 
      gcccttggc catcctgcg gctgaccag gagctgggg 
      aagagcgac ttgggggcc gcttaagcg atgagccgg 
      tggccgagt cgctcatgg acctcccca cgacggtgc 
      aggtgtatt tcagcgtgg ggcagcgcc acgaggcag 
      cgcttttcc gctccgact cgcccgcgg aacagatta 
      aagatttat tcagatctg cctccgagg ctggtcccc 
      gggacattc tgaaactgt acaacacct ccggtcaat 
      tgttgaaca tcagcggag atttgcctc ccaacacac 
      cagacagca ggtactctc tgcaagtgg tggccgcca 
      acggcttcg ctatgctcc aggaatcga ccggaaaca 
      gcctaaaag ctctggaag ctcgagtat ccgccatcg 
      aaagacagc tacgatccg aactgccgc tgcctcccg 
      ccgtcgaag agctccaga gacaagtgg aggagttcc 
      gggagaagc ttgagacta cggagagtc tgagctggc 
      tcgctttgt cccgttttt tcaagcgct cccgctttc 
      atgcaaata tcatacgtt ttagtggct tttattcga 
      tttgtaata ttttaggat tctacaagc agcttccgg 
      aacccatct cgagtgaag agtgccgcc gtttgcagt 
      accggcgaa aaagcgact cggtgaaaa aacgagtga 
      agcaaaact tcgccaaca tttgtgaag ctcaagttt 
      cggatgcga ttcgacagt ggctgagga caccgacgt 
      tcgacgcca ggccctggg aggacgaag aattgcttc 
      tcctgcttc aacaaatgt atctcgatt atgatctct 
      gttcaaagt ttgctattg acataaata cgcttagaa 
      atttcctct acgaagcct acaagaatt ataatgacg 
      tgccctttc acaatttta gacactgct tctgcgtcg 
      cacaaatga tgtacgcca tctcttgga gcgtggatt 
      tgccctcga aagtcggcg atttggaag ttttgattt 
      tgctaacat cgtgcatct gtcacgatc tggaccatc 
      ccggttaca acaacattt accaaatca acgcaaaga 
      ccgaattag cgatccgtt ataacgaca tatcgccgt 
      tagagaatc accactgta gcgtagcat tcaggatac 
      tcgaaaacg aagactgca acatattca agtctttca 
      aaagcgagg atttcaaaa cagtgcgcg agggcatca 
      tcaggtgca tattagcta cagacatgg ctcgacata 
      atgaaatcc tcaccaact tcagggaga ttacgccca 
      attttgact ataacgaca aagctcacg ttaatttgc 
      tttgtatgg tccttatca aagtgtcgg atatatcga 
      acgaagcgc ggccgatgg acgtggcgg aaccttggc 
      tcgatagac tcctgcaag aattcttca aacaaagcg 
      acgccgaga agctggaag ggttacctg tcaccccgt 
      tcatggaca gggagaaaa taacgaaac cctcgtctc 
      agtgttcat ttatcggct tcgtcctcc tcccattgt 
      tcgaagcgt tgggagact tacttactg agttacaag 
      atctcattg ttcaaccgg ttcgcgaag cgctggaat 
      attatagga gattaaacg aagcgacga gagaggagc 
      gacttcaca ggaaaagta tagtgtctg agatgacgg 
      accagcaca ccccatcca cacaaagcc ccgatagcg 
      ccgttaccg ttcccaaat cgctttcga atgccagtg 
      ttaagctga aaaagagcc tcagcttcc agcaaaata 
      ggtcgaggt cccgatcca ccgacgaag acaccgaac 
      agagcttcg gcagcggca acatgatcg gcgaggagc 
      aaagcctcg aagacgtgg tcgaagacc ccgaaagcg 
      gcgaatcgg aaaccgcca ccgaagtcg aagtctcag 
      aaaaggccc tcaagttca aaatatcga ccgaaagta 
      gcgtgggaa ccggtcgca aaagctacc ccggctcca 
      ggaaaggca gccgggaac gcgtccaac acttcaaca 
      gctctgaac tcgctcgaa tgatccaga agggcaaac 
      tgcgctccg ccggcttca gcttcgacc aacactgca 
      tgataggca acaagaagt tatccttcg acactccgg 
      agtttttgg acgagtacg ctttaacct tgaagaagc 
      ggctggacg accaatcgg acagcgagg aggagaagc 
      aatgcgtgg tctcgaaag agaccctca acttgaaca 
      agcgcaact cggagcatt tggtcaaga tcccgtcga 
      acctggagg agaaacgca gcattctaa agtgttctg 
      acaataaat ctttcacga acgcggaca aactccgga 
      tgaacaacg tgaagtcgt ccctcgaag acgacgaga 
      aactgatcg tcaacaacc ggaagaaca gtcagtcgc 
      gagtgaaca ataccagtg atagcgtta acagtattg 
      acaaggacg aagtgcgga aagtgcaag cggcggccg 
      ggagtccgc ggaacagcc gttcgattt tctcccgct 
      ttcggcagt tcaccgatc ggttcagtc tttcggtgg 
      acaaagact cgaaggtga agcacccga agaacaaca 
      acagcacgc gaaacccct tcccgcaca agaagaaga 
      agctggaga cgccgtgct gccgcagct tggaggaag 
      ttggccgtg cgagcacac tcccgaaga ccaagagca 
      agaaagcgt ggaagttcc tggtgctgg gcaaggaga 
      aggacaagt gggcgtctg acgcagctg tggataagc 
      tcgctgtgg tcgaaaccg acaatcaat ctctaccgt 
      ctacctcac agctgcaga gtaattgcg cgagtccca 
      agacgcact tggagaagt tggaaattg tgagcttga 
      aggagttca aggtggagg aaacggtgg aaaaaacag 
      gggttgaaa agggtgaag agggtggag tgatttgac 
      aagagatca atccccccg aaagggaga gtttcgcta 
      gatacattt tttgtcggt gttgtaaat ataaaccaa 
      atgtaagaa ataatgtgt aggccgttc tttttattt 
      atttatatt tatttttgc gtttagaat aactgtgta 
      cccgtctag tatctccga acatatccc ttcaataga 
      ataatcaaa agttttaac cgattattt ccgacacgg 
      cagacaaga atgttgctt cacgataaa ggtacaaat 
      gagattatc aaaattaag gcacaattg tattagata 
      ttataagtt gtaattact ctcagtcag tcctttcct 
      tgtaatatt atttattga ttacaatag cgaatcgtt 
      atgttttat aataaaaca gattttata acatttttt 
      ataagatat tgtttataa aagatattt atccatgtg 
      attctagtc tgataaaaa tgatattta gttcaatat 
      tatttttat gtgtgcatt tactaggat taggaaaat 
      cttaatatt ttcgtacca gagacatgg tcatttatt 
      tttttcgtt acctcatac ttttcaaaa tgtgctgga 
      atttgaaag tgacgatct ggtgctaaa aattgaaaa 
      ttctttgtt gttaacaga gattgttgg taattttat 
      tttgttttg tatagtttt ttcaaaaat atgaggacc 
      attgaagcc tgtaaatcc tttttatct tctaaagag 
      aaaatctat attcttcct attgctaga tgaataagt 
      gatatattt agaaaataa cactaaaat attttactt 
      aggttataa tatgtccat atgccaatg taaaataaa 
      aagatattg attaa
    • >TC000150 RB
      gtaagtaga ttttgcaaa gaatttttt tcggaggaa 
      atggatgag aaaaaaaac atgattccg aatgcacta 
      cgaacgatt gaaaggaaa ctgaagcaa aggacgact 
      ctgccgaaa tcaatgata gaattcctg tgatcagtt 
      caccaacaa aacgacaaa gaaaatttg gcggtaaaa 
      tattgagta tctgccaaa cagttaaat aagaaactg 
      aaggagtat cctagctag aaaggcagc acgtcgttt 
      caaatgggc taaatcagg ccaatagaa aaattcgta 
      ttgaaacaa aaatttggg gacatatgc gtaaacaat 
      gtgtaacga caaatgaag ataattatt taaatttat 
      tcagatctg cctccgagg ctggtcccc gggacattc 
      tgaaactgt acaacacct ccggtcaat tgttgaaca 
      tcagcggag atttgcctc ccaacacac cagacagca 
      ggtactctc tgcaagtgg tggccgcca acggcttcg 
      ctatgctcc aggaatcga ccggaaaca gcctaaaag 
      ctctggaag ctcgagtat ccgccatcg aaagacagc 
      tacgatccg aactgccgc tgcctcccg ccgtcgaag 
      agctccaga gacaagtgg aggagttcc gggagaagc 
      ttgagacta cggagagtc tgagctggc tcgctttgt 
      cccgttttt tcaagcgct cccgctttc atgcaaata 
      tcatacgtt ttagtggct tttattcga tttgtaata 
      ttttaggat tctacaagc agcttccgg aacccatct 
      cgagtgaag agtgccgcc gtttgcagt accggcgaa 
      aaagcgact cggtgaaaa aacgagtga agcaaaact 
      tcgccaaca tttgtgaag ctcaagttt cggatgcga 
      ttcgacagt ggctgagga caccgacgt tcgacgcca 
      ggccctggg aggacgaag aattgcttc tcctgcttc 
      aacaaatgt atctcgatt atgatctct gttcaaagt 
      ttgctattg acataaata cgcttagaa atttcctct 
      acgaagcct acaagaatt ataatgacg tgccctttc 
      acaatttta gacactgct tctgcgtcg cacaaatga 
      tgtacgcca tctcttgga gcgtggatt tgccctcga 
      aagtcggcg atttggaag ttttgattt tgctaacat 
      cgtgcatct gtcacgatc tggaccatc ccggttaca 
      acaacattt accaaatca acgcaaaga ccgaattag 
      cgatccgtt ataacgaca tatcgccgt tagagaatc 
      accactgta gcgtagcat tcaggatac tcgaaaacg 
      aagactgca acatattca agtctttca aaagcgagg 
      atttcaaaa cagtgcgcg agggcatca tcaggtgca 
      tattagcta cagacatgg ctcgacata atgaaatcc 
      tcaccaact tcagggaga ttacgccca attttgact 
      ataacgaca aagctcacg ttaatttgc tttgtatgg 
      tccttatca aagtgtcgg atatatcga acgaagcgc 
      ggccgatgg acgtggcgg aaccttggc tcgatagac 
      tcctgcaag aattcttca aacaaagcg acgccgaga 
      agctggaag ggttacctg tcaccccgt tcatggaca 
      gggagaaaa taacgaaac cctcgtctc agtgttcat 
      ttatcggct tcgtcctcc tcccattgt tcgaagcgt 
      tgggagact tacttactg agttacaag atctcattg 
      ttcaaccgg ttcgcgaag cgctggaat attatagga 
      gattaaacg aagcgacga gagaggagc gacttcaca 
      ggaaaagta tagtgtctg agatgacgg accagcaca 
      ccccatcca cacaaagcc ccgatagcg ccgttaccg 
      ttcccaaat cgctttcga atgccagtg ttaagctga 
      aaaagagcc tcagcttcc agcaaaata ggtcgaggt 
      cccgatcca ccgacgaag acaccgaac agagcttcg 
      gcagcggca acatgatcg gcgaggagc aaagcctcg 
      aagacgtgg tcgaagacc ccgaaagcg gcgaatcgg 
      aaaccgcca ccgaagtcg aagtctcag aaaaggccc 
      tcaagttca aaatatcga ccgaaagta gcgtgggaa 
      ccggtcgca aaagctacc ccggctcca ggaaaggca 
      gccgggaac gcgtccaac acttcaaca gctctgaac 
      tcgctcgaa tgatccaga agggcaaac tgcgctccg 
      ccggcttca gcttcgacc aacactgca tgataggca 
      acaagaagt tatccttcg acactccgg agtttttgg 
      acgagtacg ctttaacct tgaagaagc ggctggacg 
      accaatcgg acagcgagg aggagaagc aatgcgtgg 
      tctcgaaag agaccctca acttgaaca agcgcaact 
      cggagcatt tggtcaaga tcccgtcga acctggagg 
      agaaacgca gcattctaa agtgttctg acaataaat 
      ctttcacga acgcggaca aactccgga tgaacaacg 
      tgaagtcgt ccctcgaag acgacgaga aactgatcg 
      tcaacaacc ggaagaaca gtcagtcgc gagtgaaca 
      ataccagtg atagcgtta acagtattg acaaggacg 
      aagtgcgga aagtgcaag cggcggccg ggagtccgc 
      ggaacagcc gttcgattt tctcccgct ttcggcagt 
      tcaccgatc ggttcagtc tttcggtgg acaaagact 
      cgaaggtga agcacccga agaacaaca acagcacgc 
      gaaacccct tcccgcaca agaagaaga agctggaga 
      cgccgtgct gccgcagct tggaggaag ttggccgtg 
      cgagcacac tcccgaaga ccaagagca agaaagcgt 
      ggaagttcc tggtgctgg gcaaggaga aggacaagt 
      gggcgtctg acgcagctg tggataagc tcgctgtgg 
      tcgaaaccg acaatcaat ctctaccgt ctacctcac 
      agctgcaga gtaattgcg cgagtccca agacgcact 
      tggagaagt tggaaattg tgagcttga aggagttca 
      aggtggagg aaacggtgg aaaaaacag gggttgaaa 
      agggtgaag agggtggag tgatttgac aagagatca 
      atccccccg aaagggaga gtttcgcta gatacattt 
      tttgtcggt gttgtaaat ataaaccaa atgtaagaa 
      ataatgtgt aggccgttc tttttattt atttatatt 
      tatttttgc gtttagaat aactgtgta cccgtctag 
      tatctccga acatatccc ttcaataga ataatcaaa 
      agttttaac cgattattt ccgacacgg cagacaaga 
      atgttgctt cacgataaa ggtacaaat gagattatc 
      aaaattaag gcacaattg tattagata ttataagtt 
      gtaattact ctcagtcag tcctttcct tgtaatatt 
      atttattga ttacaatag cgaatcgtt atgttttat 
      aataaaaca gattttata acatttttt ataagatat 
      tgtttataa aagatattt atccatgtg attctagtc 
      tgataaaaa tgatattta gttcaatat tatttttat 
      gtgtgcatt tactaggat taggaaaat cttaatatt 
      ttcgtacca gagacatgg tcatttatt tttttcgtt 
      acctcatac ttttcaaaa tgtgctgga atttgaaag 
      tgacgatct ggtgctaaa aattgaaaa ttctttgtt 
      gttaacaga gattgttgg taattttat tttgttttg 
      tatagtttt ttcaaaaat atgaggacc attgaagcc 
      tgtaaatcc tttttatct tctaaagag aaaatctat 
      attcttcct attgctaga tgaataagt gatatattt 
      agaaaataa cactaaaat attttactt aggttataa 
      tatgtccat atgccaatg taaaataaa aagatattg 
      attaa