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Gene information
General information
TC000148 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 36955..38742
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000148
    iBeetle screen iB_02984
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000148 RA
      MMFVIFFAV LFLIYYYLT RNFKHWEKK NVPFIKPLP 
      FFGSIYDGV LMRHSIGEV FYDLYYKST KPFVGFFIL 
      DKPCLLIRD PKLIKKILV NDFQYFYDR NAVNNKRDD 
      PISTHILFI LKNPDWREL RTKMTPVFT SSKIKIMSE 
      LIENASHEM TNYLNNHIK DYNSVEMRD VCLKFTVDV 
      IGSTIFGVQ ANSFKDENS QFSSVAKRL IDWDDIVTA 
      FRFRCYLLA PLFVNLFRM KLFPPDCVN FLKNTFLDI 
      MDKRSVSNK SRNDLIDIL LQMKNDNRN FIEGDILVS 
      QALMFFVAG FETTSSTMG FALYEFARN PDIQDKIRN 
      EIKDISDKY GDIKYDSLK EMEYLDMCV KEVLRKYPV 
      VPFLDRKCN TTYTIPDTN VTIDKDTPI FIPSLALHY 
      DPQYFPNAD IFDPERFSS NNKTGIDSF AYLPFGEGP 
      RNCIGARFG LLTAKLGLV HILKEFVVS CNEKSNEKI 
      KFNPKGMVL SALNGINLK LSKVEKAT
    • >TC000148 RB
      MMFVIFFAV LFLIYYYLT RNFKHWEKK NVPFIKPLP 
      FFGSIYDGV LMRHSIGEV FYDLYYKST KPFVGFFIL 
      DKPCLLIRD PKLIKKILV NDFQYFYDR NAVNNKRDD 
      PISTHILFI LKNPDWREL RTKMTPVFT SSKIKIMSE 
      LIENASHEM TNYLNNHIK DYNSVEMRD VCLKFTVDV 
      IGSTIFGVQ ANSFKDENS QFSSVAKRL IDWDDIVTA 
      FRFRCYLLA PLFVNLFRM KLFPPDCVN FLKNTFLDI 
      MDKRSVSNK SRNDLIDIL LQMKNDNRN FIEGDILVS 
      QALMFFVAG FETTSSTMG FALYEFARN PDIQDKIRN 
      EIKDISDKY GDIKYDSLK EMEYLDMCV KGITNYGNL 
      FNFFKFVIL EVLRKYPVV PFLDRKCNT TYTIPDTNV 
      TIDKDTPIF IPSLALHYD PQYFPNADI FDPERFSSN 
      NKTGIDSFA YLPFGEGPR NCIGARFGL LTAKLGLVH 
      ILKEFVVSC NEKSNEKIK FNPKGMVLS ALNGINLKL 
      SKVEKAT
    CDS sequence
    • >TC000148 RA
      atgatgttt gtaattttc tttgctgtg ttattcctc 
      atctattac tatttgacc cgaaatttc aaacactgg 
      gaaaagaaa aatgtgcct ttcatcaag cctttgcca 
      ttttttggt agtatttac gatggtgtt ttgatgcgt 
      cactcgata ggagaagta ttttacgac ctttattac 
      aaatcaacg aaacctttc gttggattt tttatcctg 
      gataagcct tgtttacta atcagagat ccgaaattg 
      attaagaag attctagta aacgatttt caatatttc 
      tatgacaga aatgccgta aataataaa agggatgac 
      ccaattagt acccacatt ttattcata ttgaaaaat 
      cccgactgg agggaactt aggaccaaa atgacgcca 
      gtttttacc tcttcaaaa ataaaaata atgtcggaa 
      ttaatcgaa aatgcttcg catgaaatg accaactat 
      ttaaacaat catatcaag gattacaat tcagttgaa 
      atgagagat gtttgtttg aaattcacc gtggatgtg 
      attggttca actattttt ggagtgcaa gctaattct 
      ttcaaagat gaaaattcg caatttagt tcagtggcc 
      aagagactg attgattgg gacgatatt gtcacagca 
      tttcggttc aggtgttat cttttggcc cctttgttt 
      gttaacttg tttcgaatg aaactcttt cctcctgat 
      tgtgtaaat tttttgaag aacactttt ttggatatc 
      atggataag agatcggtt tcaaacaag tccagaaat 
      gatttaatt gatatttta ctacaaatg aagaatgat 
      aatcgcaac ttcatagaa ggagatatt ttagtgtct 
      caagcactc atgttcttt gttgctggc tttgagaca 
      acgagctca acaatgggc tttgcattg tatgaattt 
      gcacgtaat cctgacatt caggacaaa ataagaaac 
      gaaataaaa gacatttcg gacaaatac ggcgatata 
      aaatatgat tccttaaaa gaaatggaa tatttggac 
      atgtgtgtt aaggaagtt ttacgaaaa tatccagtt 
      gtgccattc cttgacaga aaatgtaac accacctac 
      acgatacca gacaccaat gttacgatt gataaagac 
      actcctatt ttcatccca agtttagct ttgcactat 
      gatccccaa tattttccg aatgctgat atttttgat 
      ccggagcgc ttttccagt aataacaaa actggaatc 
      gacagtttt gcttactta ccatttggg gaaggacca 
      cgtaattgt ataggtgca agatttggt ttgctaacc 
      gcaaaatta ggtctagtg catattttg aaagagttt 
      gttgtgagt tgtaatgag aagagtaac gaaaagatt 
      aaatttaat cccaaaggg atggtcctg tcggcgtta 
      aatggaatt aatttgaaa ttaagcaaa gttgaaaaa 
      gcaacttaa
    • >TC000148 RB
      atgatgttt gtaattttc tttgctgtg ttattcctc 
      atctattac tatttgacc cgaaatttc aaacactgg 
      gaaaagaaa aatgtgcct ttcatcaag cctttgcca 
      ttttttggt agtatttac gatggtgtt ttgatgcgt 
      cactcgata ggagaagta ttttacgac ctttattac 
      aaatcaacg aaacctttc gttggattt tttatcctg 
      gataagcct tgtttacta atcagagat ccgaaattg 
      attaagaag attctagta aacgatttt caatatttc 
      tatgacaga aatgccgta aataataaa agggatgac 
      ccaattagt acccacatt ttattcata ttgaaaaat 
      cccgactgg agggaactt aggaccaaa atgacgcca 
      gtttttacc tcttcaaaa ataaaaata atgtcggaa 
      ttaatcgaa aatgcttcg catgaaatg accaactat 
      ttaaacaat catatcaag gattacaat tcagttgaa 
      atgagagat gtttgtttg aaattcacc gtggatgtg 
      attggttca actattttt ggagtgcaa gctaattct 
      ttcaaagat gaaaattcg caatttagt tcagtggcc 
      aagagactg attgattgg gacgatatt gtcacagca 
      tttcggttc aggtgttat cttttggcc cctttgttt 
      gttaacttg tttcgaatg aaactcttt cctcctgat 
      tgtgtaaat tttttgaag aacactttt ttggatatc 
      atggataag agatcggtt tcaaacaag tccagaaat 
      gatttaatt gatatttta ctacaaatg aagaatgat 
      aatcgcaac ttcatagaa ggagatatt ttagtgtct 
      caagcactc atgttcttt gttgctggc tttgagaca 
      acgagctca acaatgggc tttgcattg tatgaattt 
      gcacgtaat cctgacatt caggacaaa ataagaaac 
      gaaataaaa gacatttcg gacaaatac ggcgatata 
      aaatatgat tccttaaaa gaaatggaa tatttggac 
      atgtgtgtt aagggtata acgaattat ggtaactta 
      tttaatttt tttaaattc gtaatttta gaagtttta 
      cgaaaatat ccagttgtg ccattcctt gacagaaaa 
      tgtaacacc acctacacg ataccagac accaatgtt 
      acgattgat aaagacact cctattttc atcccaagt 
      ttagctttg cactatgat ccccaatat tttccgaat 
      gctgatatt tttgatccg gagcgcttt tccagtaat 
      aacaaaact ggaatcgac agttttgct tacttacca 
      tttggggaa ggaccacgt aattgtata ggtgcaaga 
      tttggtttg ctaaccgca aaattaggt ctagtgcat 
      attttgaaa gagtttgtt gtgagttgt aatgagaag 
      agtaacgaa aagattaaa tttaatccc aaagggatg 
      gtcctgtcg gcgttaaat ggaattaat ttgaaatta 
      agcaaagtt gaaaaagca acttaa
    mRNA sequence
    • >TC000148 RA
      aaaattaaa attcctccc atccattca gatgtatct 
      cctttggcc gtgaaccgt ttgcgtata ataaagtga 
      aggtgacga tgatgtttg taattttct ttgctgtgt 
      tattcctca tctattact atttgaccc gaaatttca 
      aacactggg aaaagaaaa atgtgcctt tcatcaagc 
      ctttgccat tttttggta gtatttacg atggtgttt 
      tgatgcgtc actcgatag gagaagtat tttacgacc 
      tttattaca aatcaacga aacctttcg ttggatttt 
      ttatcctgg ataagcctt gtttactaa tcagagatc 
      cgaaattga ttaagaaga ttctagtaa acgattttc 
      aatatttct atgacagaa atgccgtaa ataataaaa 
      gggatgacc caattagta cccacattt tattcatat 
      tgaaaaatc ccgactgga gggaactta ggaccaaaa 
      tgacgccag tttttacct cttcaaaaa taaaaataa 
      tgtcggaat taatcgaaa atgcttcgc atgaaatga 
      ccaactatt taaacaatc atatcaagg attacaatt 
      cagttgaaa tgagagatg tttgtttga aattcaccg 
      tggatgtga ttggttcaa ctatttttg gagtgcaag 
      ctaattctt tcaaagatg aaaattcgc aatttagtt 
      cagtggcca agagactga ttgattggg acgatattg 
      tcacagcat ttcggttca ggtgttatc ttttggccc 
      ctttgtttg ttaacttgt ttcgaatga aactctttc 
      ctcctgatt gtgtaaatt ttttgaaga acacttttt 
      tggatatca tggataaga gatcggttt caaacaagt 
      ccagaaatg atttaattg atattttac tacaaatga 
      agaatgata atcgcaact tcatagaag gagatattt 
      tagtgtctc aagcactca tgttctttg ttgctggct 
      ttgagacaa cgagctcaa caatgggct ttgcattgt 
      atgaatttg cacgtaatc ctgacattc aggacaaaa 
      taagaaacg aaataaaag acatttcgg acaaatacg 
      gcgatataa aatatgatt ccttaaaag aaatggaat 
      atttggaca tgtgtgtta aggaagttt tacgaaaat 
      atccagttg tgccattcc ttgacagaa aatgtaaca 
      ccacctaca cgataccag acaccaatg ttacgattg 
      ataaagaca ctcctattt tcatcccaa gtttagctt 
      tgcactatg atccccaat attttccga atgctgata 
      tttttgatc cggagcgct tttccagta ataacaaaa 
      ctggaatcg acagttttg cttacttac catttgggg 
      aaggaccac gtaattgta taggtgcaa gatttggtt 
      tgctaaccg caaaattag gtctagtgc atattttga 
      aagagtttg ttgtgagtt gtaatgaga agagtaacg 
      aaaagatta aatttaatc ccaaaggga tggtcctgt 
      cggcgttaa atggaatta atttgaaat taagcaaag 
      ttgaaaaag caacttaat aaaatattt ttggcacaa 
      aatttcgtg tagttttag tacttg
    • >TC000148 RB
      aaaattaaa attcctccc atccattca gatgtatct 
      cctttggcc gtgaaccgt ttgcgtata ataaagtga 
      aggtgacga tgatgtttg taattttct ttgctgtgt 
      tattcctca tctattact atttgaccc gaaatttca 
      aacactggg aaaagaaaa atgtgcctt tcatcaagc 
      ctttgccat tttttggta gtatttacg atggtgttt 
      tgatgcgtc actcgatag gagaagtat tttacgacc 
      tttattaca aatcaacga aacctttcg ttggatttt 
      ttatcctgg ataagcctt gtttactaa tcagagatc 
      cgaaattga ttaagaaga ttctagtaa acgattttc 
      aatatttct atgacagaa atgccgtaa ataataaaa 
      gggatgacc caattagta cccacattt tattcatat 
      tgaaaaatc ccgactgga gggaactta ggaccaaaa 
      tgacgccag tttttacct cttcaaaaa taaaaataa 
      tgtcggaat taatcgaaa atgcttcgc atgaaatga 
      ccaactatt taaacaatc atatcaagg attacaatt 
      cagttgaaa tgagagatg tttgtttga aattcaccg 
      tggatgtga ttggttcaa ctatttttg gagtgcaag 
      ctaattctt tcaaagatg aaaattcgc aatttagtt 
      cagtggcca agagactga ttgattggg acgatattg 
      tcacagcat ttcggttca ggtgttatc ttttggccc 
      ctttgtttg ttaacttgt ttcgaatga aactctttc 
      ctcctgatt gtgtaaatt ttttgaaga acacttttt 
      tggatatca tggataaga gatcggttt caaacaagt 
      ccagaaatg atttaattg atattttac tacaaatga 
      agaatgata atcgcaact tcatagaag gagatattt 
      tagtgtctc aagcactca tgttctttg ttgctggct 
      ttgagacaa cgagctcaa caatgggct ttgcattgt 
      atgaatttg cacgtaatc ctgacattc aggacaaaa 
      taagaaacg aaataaaag acatttcgg acaaatacg 
      gcgatataa aatatgatt ccttaaaag aaatggaat 
      atttggaca tgtgtgtta agggtataa cgaattatg 
      gtaacttat ttaattttt ttaaattcg taattttag 
      aagttttac gaaaatatc cagttgtgc cattccttg 
      acagaaaat gtaacacca cctacacga taccagaca 
      ccaatgtta cgattgata aagacactc ctattttca 
      tcccaagtt tagctttgc actatgatc cccaatatt 
      ttccgaatg ctgatattt ttgatccgg agcgctttt 
      ccagtaata acaaaactg gaatcgaca gttttgctt 
      acttaccat ttggggaag gaccacgta attgtatag 
      gtgcaagat ttggtttgc taaccgcaa aattaggtc 
      tagtgcata ttttgaaag agtttgttg tgagttgta 
      atgagaaga gtaacgaaa agattaaat ttaatccca 
      aagggatgg tcctgtcgg cgttaaatg gaattaatt 
      tgaaattaa gcaaagttg aaaaagcaa cttaataaa 
      atatttttg gcacaaaat ttcgtgtag ttttagtac 
      ttg