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Gene information
General information
TC000147 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1035403..1038370
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000147
    iBeetle screen iB_02983

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000147 RA
      MKLLMYFKK IYNLIRLQR EQGGGFEPI LNFLNKILR 
      NLDAREATI KKQSSDVQR VLDFATKHI ARDKAEKEL 
      LQKEASELE DAMKFIETQ LANKKDINE FALKQQLMR 
      IPEKTQHVV NDLKVDHVT EVFSTKEKE FESDASNQQ 
      EFVDQCVDK FLQGLKPNL VEYNKHIDE TFSKVKTVA 
      ETVKTSVLE KTYNDSLSL EEIINAAKE ESDDYAFKS 
      MNIVKQTLN KLESCSNDI CLTGHVGDT PVKTSHGHF 
      DPENSENIP PPKGNLFQN KDDPLH
    CDS sequence
    • >TC000147 RA
      atgaaatta ctcatgtat ttcaaaaaa atttataat 
      ctcatacgc ctccaaaga gagcaagga ggcggtttt 
      gaacctata ttgaatttc cttaataaa attttgcgt 
      aatttggac gctcgagaa gctacaata aagaagcag 
      agtagtgac gtacaacgt gtacttgat tttgcgact 
      aagcacata gcccgtgat aaagctgaa aaggagttg 
      ctccaaaag gaagcttca gagctagaa gatgctatg 
      aaattcata gagacacag cttgcaaac aaaaaggat 
      attaacgaa tttgctcta aagcaacaa ctgatgcgt 
      attcccgaa aaaacgcag catgttgtc aacgatctt 
      aaagttgat catgtgact gaagtattc tccactaag 
      gaaaaggag tttgaatct gatgcttcc aatcagcag 
      gaattcgtc gatcaatgt gttgacaaa tttttgcaa 
      ggtttaaaa ccaaattta gttgagtac aataagcat 
      attgatgaa actttctct aaggtgaaa acagtcgct 
      gaaactgtt aagacctcc gttctggag aagacttac 
      aatgacagc ctttcgctt gaagaaata attaatgcc 
      gcaaaagaa gagagtgat gactatgcg tttaaatct 
      atgaacatt gttaagcaa acgttaaat aaactggaa 
      tcgtgcagc aacgatatt tgcttgact ggacacgtg 
      ggagatact cctgtcaag acctctcac ggtcacttt 
      gatcctgag aatagtgaa aatattcct ccaccgaaa 
      gggaatctg tttcagaat aaggatgat ccattgcac 
      tga
    mRNA sequence
    • >TC000147 RA
      tcaaaacta gccgttatg aaattactc atgtatttc 
      aaaaaaatt tataatctc atacgcctc caaagagag 
      caaggaggc ggttttgaa cctatattg aatttcctt 
      aataaaatt ttgcgtaat ttggacgct cgagaagct 
      acaataaag aagcagagt agtgacgta caacgtgta 
      cttgatttt gcgactaag cacatagcc cgtgataaa 
      gctgaaaag gagttgctc caaaaggaa gcttcagag 
      ctagaagat gctatgaaa ttcatagag acacagctt 
      gcaaacaaa aaggatatt aacgaattt gctctaaag 
      caacaactg atgcgtatt cccgaaaaa acgcagcat 
      gttgtcaac gatcttaaa gttgatcat gtgactgaa 
      gtattctcc actaaggaa aaggagttt gaatctgat 
      gcttccaat cagcaggaa ttcgtcgat caatgtgtt 
      gacaaattt ttgcaaggt ttaaaacca aatttagtt 
      gagtacaat aagcatatt gatgaaact ttctctaag 
      gtgaaaaca gtcgctgaa actgttaag acctccgtt 
      ctggagaag acttacaat gacagcctt tcgcttgaa 
      gaaataatt aatgccgca aaagaagag agtgatgac 
      tatgcgttt aaatctatg aacattgtt aagcaaacg 
      ttaaataaa ctggaatcg tgcagcaac gatatttgc 
      ttgactgga cacgtggga gatactcct gtcaagacc 
      tctcacggt cactttgat cctgagaat agtgaaaat 
      attcctcca ccgaaaggg aatctgttt cagaataag 
      gatgatcca ttgcactga agttttttc taaatgttt 
      ttttgttgt tttgtttca cttttttag tttggaatt 
      ttagatgta gtttgttct gttcagtta ttttgaaat 
      aaaccattt tgcattaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY