| General information | |||
| TC000124 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 10842521..10852433 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC000124 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02981 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC000124 RA
MPDFLAENN ICGQTILQL VSRGNAIIA ELLRLKDYI PQTYRLGSK EEVQKYGEI IMDFSYFKI EEAQNYKIE NSELLQDLD EEFRDNHIE MINRFYLTF ESIHTYITD LNHFLDELE EGLYIHQSL ETIFADMEG KQLLCEALF LYGLMLLII DTYIEGPIR ERLLVSYYR YTPQRSDTQ SQIDDVCRL LRDTGFHTA KKPNNYPED YFRRVPLKP KYVDIVIGH LRSDDIYGQ LSVYPLPKH RSTALAHQA SMLYVCLFF SPNILHSQT AIMREIVDK YFPDNWVIS IYMGFIVNL AESWECFKA AKMALNNTL ETVNIKGYA SSYGSSVLP LLKATNLKL KEGNITSDN LLKDVNNII NLLRDCNFT IRWLMLHTL LKPGKNDKS KKLKQLREL VITESRLES VQLFKLLLN TSQLELITR EIYKNLLLE KDNQWSELK SESFKSLVE LSEVFSGAK PLTRIPKNE NLQNWFLEI SKQVDSLCS QDAASSRKI VQLIQALEE VQEFHQLES NMQVVQFLS ETRKFLHQM IRNMNIKED VLITLQIIG DLSYAWELI DSFTPIMQF GIKKEPTLV IKLRAIFLK LSSALEVPL LRINQAHSE DLISVSQYY SSELEIYVR KVLQIIPKM MFEKMARVI EIQTCILKE LPTRLDKDK LKDFAQLNE RFEFAELTH SISVFSQGM RMMKSTLVG VICLDPKQL LEDGIRKEL VLYISKALH TGLIFGQRV KVEELEGKL EAVGVIMDG YKRSFEYIQ DYININGLK IWQEEVTRI INYNVEQEC NGFLRNKIH TWESAFQSR YVPIPLYPS TDSFSENFI GRLAREIIK LTDPKQSVY LEQTSTWYD IKSHKPIFN KETIALVAS AIEISGLVG LDRLFSFMI ISNLQKLSG YLEMKNAKT NAWHNVLAS IHKDMIGSE NVNNPIKFY QNCVNRTTK IWNEFLDCI LLVGQLQLL RNLIAFHLN KSCKFNAKN LESSLRSLN KALLLDIVN GECAPSEDI MSKLSHYFD YSGLNNPFN KIYITSKFD DNHYITIFV FAIAHFSKL FFLRQLGNA NKRNADQID GTAFTVAIH TLVKQFHNK LNESFIKLL SNYVVQLTR YSISIKNTE LPLEVSLAM SFLETYTNY SESSRKCLK EEIPDEILH LQQTLVASA S
- >TC000124 RA
atgccggat tttctggca gaaaataat atttgtggc cagacaatt ctccagttg gtctcacga ggcaatgcc atcattgcc gaattgctc cgcttgaaa gactacatc ccccaaact tacagattg gggagtaag gaagaggtc caaaaatac ggcgagata atcatggac tttagctat tttaaaatc gaggaagcg cagaattac aaaattgag aattcggag ttgttgcaa gatttggat gaggagttt cgggataat cacatcgag atgattaat cggttttat ctcacgttt gaaagcatc cacacttat attacggat ttgaaccac tttctcgac gagttggag gaagggtta tacatccat caaagtcta gagacgatt tttgccgac atggagggg aaacaactg ctttgtgaa gctttattt ttgtacggt ttaatgctg ctaatcata gacacgtat attgagggc cccatccgt gagagactc ctagtgtct tactatcgc tacactccg caaagaagc gacactcaa agccaaatc gacgatgtt tgccgttta ttacgcgac acaggcttt cacacggcg aaaaaaccc aataattat cctgaggat tatttccga cgcgttcct cttaaacca aaatacgtt gacatcgta atcggtcat ttgcgctcc gacgacatc tacggccaa ttatcagtg taccccctt cccaaacac cgaagcacc gcactcgcc caccaagcc tcaatgctt tacgtttgt ctcttcttc tcaccgaat attttacat tcccaaacg gcaattatg cgcgaaatc gtcgataaa tacttcccg gataattgg gtcatttcc atttacatg ggttttatc gtaaatttg gccgaaagc tgggagtgc ttcaaggcg gcgaaaatg gcccttaac aacaccctt gaaaccgta aacatcaaa ggttacgcc agttcgtac ggaagcagc gttttgccc ctcctcaaa gccacaaat ttgaagctt aaagaaggc aacatcact agtgacaac ctcctcaaa gacgtcaac aacataata aatcttttg agggattgc aacttcacg attcgatgg ctgatgcta cacacgctc ctcaaacct ggcaaaaac gacaaaagc aaaaaactg aaacaattg agagagctg gttataacc gaatcgcgg ttagagtca gttcaactg tttaaactg ctcctcaat acgtcacag ttggagtta atcacgcgt gaaatttac aaaaacttg ttgctggaa aaagacaat caatggtcg gaattgaaa agtgagagt tttaagagt ttggttgag ttgagtgag gttttcagt ggcgctaaa cctttgacc cgaattccg aaaaatgag aatttgcaa aattggttt ttggaaatt tcgaaacaa gtcgattcg ttgtgttca caagacgca gcttcgtct cgtaaaatc gttcaactg attcaagca cttgaggaa gtgcaggag tttcaccaa ctcgagtcc aacatgcaa gtggttcag tttttgtcc gaaactcgc aaattcctg caccaaatg atccgtaac atgaacata aaagaggat gttttgatt acgttgcaa ataatcggc gatttgagc tacgcctgg gaattgatc gattccttc actcctatc atgcaattc gggattaaa aaagagcca actttggtc attaaactc cgggcgatt ttcctcaaa ctctcctca gcgcttgaa gtcccgctt ttgcgcatc aatcaagca cacagtgag gatttgatt tcagtgtcg caatattac agtagcgag cttgaaatc tatgtcagg aaggtgttg caaataatt ccgaaaatg atgtttgag aaaatggct cgtgtgatt gagattcaa acttgtatc ttgaaggaa ttgcccaca aggctggat aaggataag ttgaaggat tttgcacag ttgaatgaa cggttcgag tttgctgaa ctgacgcat tcgatttcg gtttttagt caagggatg agaatgatg aagagtacg ctggttgga gtgatttgt ttagatccg aaacagttg ctcgaagat gggattcgg aaggagttg gtgttatat atctcgaag gcgttgcat acgggcttg atttttggg caaagggtt aaagttgag gagttggag ggcaagttg gaggccgtg ggggttatt atggatggg tataagagg tcgtttgag tatatacag gattacatc aacatcaac ggtttaaaa atctggcaa gaggaagtg actcgaatc atcaactac aacgtggag caggaatgc aacggcttt ttgcgcaac aaaatccac acttgggaa agcgctttc cagagccgt tacgtccca ataccgctc tacccaagt acggactct tttagcgaa aatttcatc gggcgatta gcgcgcgaa ataatcaaa ttgaccgat cccaaacag tccgtttat ctcgaacaa acatcgact tggtacgac atcaaaagt cacaaacca atcttcaac aaagaaacc atagctcta gtagcgtct gctatcgaa atttcgggc ttggtgggc cttgaccgg ctcttctcc ttcatgatt atttcaaat ttgcaaaaa ttgtccggg tatttggaa atgaaaaac gccaaaacg aacgcatgg cataacgtt ttggcgagt attcataaa gatatgatc gggagcgaa aatgtgaac aaccccatc aagttttac cagaactgt gttaatcga acgacgaaa atttggaac gagtttctt gattgcatt ttgttggtc ggacaattg cagcttttg aggaactta atcgctttt catttgaat aaatcctgc aagtttaat gcgaaaaat ttagaatcg tctctacgt tcactcaat aaggctttg ttactcgac attgtgaac ggcgagtgt gccccaagt gaggacatc atgtccaaa ctatcgcat tatttcgat tattcgggc ctgaataat cccttcaac aaaatatac atcacgtca aagtttgac gataatcat tacattact attttcgtt tttgcgatt gcacatttt agtaaactt ttttttcta cgtcagtta ggaaacgcc aacaaacga aatgcggat caaatcgac ggaaccgca tttactgtc gcaatccac acccttgtc aaacaattc cataataaa ttaaacgaa agtttcatc aaattatta tccaattat gtcgtacag ttgactaga tatagcata agtataaaa aataccgaa ttgcctttg gaggtgtca ctagctatg agttttctg gaaacgtac accaattac tcagagtct tcccgaaag tgtttgaaa gaagaaatt cccgatgaa attttgcat ttacaacaa actctagtc gcctctgct tcctag
- >TC000124 RA
aacctgtta aacaactgt tatcaaaag tgaggttgt ctgtgatct tttttgatg taaataaac aagattttt tcacgaaaa tgccggatt ttctggcag aaaataata tttgtggcc agacaattc tccagttgg tctcacgag gcaatgcca tcattgccg aattgctcc gcttgaaag actacatcc cccaaactt acagattgg ggagtaagg aagaggtcc aaaaatacg gcgagataa tcatggact ttagctatt ttaaaatcg aggaagcgc agaattaca aaattgaga attcggagt tgttgcaag atttggatg aggagtttc gggataatc acatcgaga tgattaatc ggttttatc tcacgtttg aaagcatcc acacttata ttacggatt tgaaccact ttctcgacg agttggagg aagggttat acatccatc aaagtctag agacgattt ttgccgaca tggagggga aacaactgc tttgtgaag ctttatttt tgtacggtt taatgctgc taatcatag acacgtata ttgagggcc ccatccgtg agagactcc tagtgtctt actatcgct acactccgc aaagaagcg acactcaaa gccaaatcg acgatgttt gccgtttat tacgcgaca caggctttc acacggcga aaaaaccca ataattatc ctgaggatt atttccgac gcgttcctc ttaaaccaa aatacgttg acatcgtaa tcggtcatt tgcgctccg acgacatct acggccaat tatcagtgt acccccttc ccaaacacc gaagcaccg cactcgccc accaagcct caatgcttt acgtttgtc tcttcttct caccgaata ttttacatt cccaaacgg caattatgc gcgaaatcg tcgataaat acttcccgg ataattggg tcatttcca tttacatgg gttttatcg taaatttgg ccgaaagct gggagtgct tcaaggcgg cgaaaatgg cccttaaca acacccttg aaaccgtaa acatcaaag gttacgcca gttcgtacg gaagcagcg ttttgcccc tcctcaaag ccacaaatt tgaagctta aagaaggca acatcacta gtgacaacc tcctcaaag acgtcaaca acataataa atcttttga gggattgca acttcacga ttcgatggc tgatgctac acacgctcc tcaaacctg gcaaaaacg acaaaagca aaaaactga aacaattga gagagctgg ttataaccg aatcgcggt tagagtcag ttcaactgt ttaaactgc tcctcaata cgtcacagt tggagttaa tcacgcgtg aaatttaca aaaacttgt tgctggaaa aagacaatc aatggtcgg aattgaaaa gtgagagtt ttaagagtt tggttgagt tgagtgagg ttttcagtg gcgctaaac ctttgaccc gaattccga aaaatgaga atttgcaaa attggtttt tggaaattt cgaaacaag tcgattcgt tgtgttcac aagacgcag cttcgtctc gtaaaatcg ttcaactga ttcaagcac ttgaggaag tgcaggagt ttcaccaac tcgagtcca acatgcaag tggttcagt ttttgtccg aaactcgca aattcctgc accaaatga tccgtaaca tgaacataa aagaggatg ttttgatta cgttgcaaa taatcggcg atttgagct acgcctggg aattgatcg attccttca ctcctatca tgcaattcg ggattaaaa aagagccaa ctttggtca ttaaactcc gggcgattt tcctcaaac tctcctcag cgcttgaag tcccgcttt tgcgcatca atcaagcac acagtgagg atttgattt cagtgtcgc aatattaca gtagcgagc ttgaaatct atgtcagga aggtgttgc aaataattc cgaaaatga tgtttgaga aaatggctc gtgtgattg agattcaaa cttgtatct tgaaggaat tgcccacaa ggctggata aggataagt tgaaggatt ttgcacagt tgaatgaac ggttcgagt ttgctgaac tgacgcatt cgatttcgg tttttagtc aagggatga gaatgatga agagtacgc tggttggag tgatttgtt tagatccga aacagttgc tcgaagatg ggattcgga aggagttgg tgttatata tctcgaagg cgttgcata cgggcttga tttttgggc aaagggtta aagttgagg agttggagg gcaagttgg aggccgtgg gggttatta tggatgggt ataagaggt cgtttgagt atatacagg attacatca acatcaacg gtttaaaaa tctggcaag aggaagtga ctcgaatca tcaactaca acgtggagc aggaatgca acggctttt tgcgcaaca aaatccaca cttgggaaa gcgctttcc agagccgtt acgtcccaa taccgctct acccaagta cggactctt ttagcgaaa atttcatcg ggcgattag cgcgcgaaa taatcaaat tgaccgatc ccaaacagt ccgtttatc tcgaacaaa catcgactt ggtacgaca tcaaaagtc acaaaccaa tcttcaaca aagaaacca tagctctag tagcgtctg ctatcgaaa tttcgggct tggtgggcc ttgaccggc tcttctcct tcatgatta tttcaaatt tgcaaaaat tgtccgggt atttggaaa tgaaaaacg ccaaaacga acgcatggc ataacgttt tggcgagta ttcataaag atatgatcg ggagcgaaa atgtgaaca accccatca agttttacc agaactgtg ttaatcgaa cgacgaaaa tttggaacg agtttcttg attgcattt tgttggtcg gacaattgc agcttttga ggaacttaa tcgcttttc atttgaata aatcctgca agtttaatg cgaaaaatt tagaatcgt ctctacgtt cactcaata aggctttgt tactcgaca ttgtgaacg gcgagtgtg ccccaagtg aggacatca tgtccaaac tatcgcatt atttcgatt attcgggcc tgaataatc ccttcaaca aaatataca tcacgtcaa agtttgacg ataatcatt acattacta ttttcgttt ttgcgattg cacatttta gtaaacttt tttttctac gtcagttag gaaacgcca acaaacgaa atgcggatc aaatcgacg gaaccgcat ttactgtcg caatccaca cccttgtca aacaattcc ataataaat taaacgaaa gtttcatca aattattat ccaattatg tcgtacagt tgactagat atagcataa gtataaaaa ataccgaat tgcctttgg aggtgtcac tagctatga gttttctgg aaacgtaca ccaattact cagagtctt cccgaaagt gtttgaaag aagaaattc ccgatgaaa ttttgcatt tacaacaaa ctctagtcg cctctgctt cctagactg attatttag gggtcgcat tgtgtggcg ccatctgtt attttttta ataaaatgt aatttttca ctaaaaagt attacgtta ttttatgca ttgattatt ttattgatg cgttccatc acagaaaaa aactctttt taaaaatat taaatttga tccatcttg
