Status
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Gene information
General information
TC000124 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10842521..10852433
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000124
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02981

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000124 RA
      MPDFLAENN ICGQTILQL VSRGNAIIA ELLRLKDYI 
      PQTYRLGSK EEVQKYGEI IMDFSYFKI EEAQNYKIE 
      NSELLQDLD EEFRDNHIE MINRFYLTF ESIHTYITD 
      LNHFLDELE EGLYIHQSL ETIFADMEG KQLLCEALF 
      LYGLMLLII DTYIEGPIR ERLLVSYYR YTPQRSDTQ 
      SQIDDVCRL LRDTGFHTA KKPNNYPED YFRRVPLKP 
      KYVDIVIGH LRSDDIYGQ LSVYPLPKH RSTALAHQA 
      SMLYVCLFF SPNILHSQT AIMREIVDK YFPDNWVIS 
      IYMGFIVNL AESWECFKA AKMALNNTL ETVNIKGYA 
      SSYGSSVLP LLKATNLKL KEGNITSDN LLKDVNNII 
      NLLRDCNFT IRWLMLHTL LKPGKNDKS KKLKQLREL 
      VITESRLES VQLFKLLLN TSQLELITR EIYKNLLLE 
      KDNQWSELK SESFKSLVE LSEVFSGAK PLTRIPKNE 
      NLQNWFLEI SKQVDSLCS QDAASSRKI VQLIQALEE 
      VQEFHQLES NMQVVQFLS ETRKFLHQM IRNMNIKED 
      VLITLQIIG DLSYAWELI DSFTPIMQF GIKKEPTLV 
      IKLRAIFLK LSSALEVPL LRINQAHSE DLISVSQYY 
      SSELEIYVR KVLQIIPKM MFEKMARVI EIQTCILKE 
      LPTRLDKDK LKDFAQLNE RFEFAELTH SISVFSQGM 
      RMMKSTLVG VICLDPKQL LEDGIRKEL VLYISKALH 
      TGLIFGQRV KVEELEGKL EAVGVIMDG YKRSFEYIQ 
      DYININGLK IWQEEVTRI INYNVEQEC NGFLRNKIH 
      TWESAFQSR YVPIPLYPS TDSFSENFI GRLAREIIK 
      LTDPKQSVY LEQTSTWYD IKSHKPIFN KETIALVAS 
      AIEISGLVG LDRLFSFMI ISNLQKLSG YLEMKNAKT 
      NAWHNVLAS IHKDMIGSE NVNNPIKFY QNCVNRTTK 
      IWNEFLDCI LLVGQLQLL RNLIAFHLN KSCKFNAKN 
      LESSLRSLN KALLLDIVN GECAPSEDI MSKLSHYFD 
      YSGLNNPFN KIYITSKFD DNHYITIFV FAIAHFSKL 
      FFLRQLGNA NKRNADQID GTAFTVAIH TLVKQFHNK 
      LNESFIKLL SNYVVQLTR YSISIKNTE LPLEVSLAM 
      SFLETYTNY SESSRKCLK EEIPDEILH LQQTLVASA 
      S
    CDS sequence
    • >TC000124 RA
      atgccggat tttctggca gaaaataat atttgtggc 
      cagacaatt ctccagttg gtctcacga ggcaatgcc 
      atcattgcc gaattgctc cgcttgaaa gactacatc 
      ccccaaact tacagattg gggagtaag gaagaggtc 
      caaaaatac ggcgagata atcatggac tttagctat 
      tttaaaatc gaggaagcg cagaattac aaaattgag 
      aattcggag ttgttgcaa gatttggat gaggagttt 
      cgggataat cacatcgag atgattaat cggttttat 
      ctcacgttt gaaagcatc cacacttat attacggat 
      ttgaaccac tttctcgac gagttggag gaagggtta 
      tacatccat caaagtcta gagacgatt tttgccgac 
      atggagggg aaacaactg ctttgtgaa gctttattt 
      ttgtacggt ttaatgctg ctaatcata gacacgtat 
      attgagggc cccatccgt gagagactc ctagtgtct 
      tactatcgc tacactccg caaagaagc gacactcaa 
      agccaaatc gacgatgtt tgccgttta ttacgcgac 
      acaggcttt cacacggcg aaaaaaccc aataattat 
      cctgaggat tatttccga cgcgttcct cttaaacca 
      aaatacgtt gacatcgta atcggtcat ttgcgctcc 
      gacgacatc tacggccaa ttatcagtg taccccctt 
      cccaaacac cgaagcacc gcactcgcc caccaagcc 
      tcaatgctt tacgtttgt ctcttcttc tcaccgaat 
      attttacat tcccaaacg gcaattatg cgcgaaatc 
      gtcgataaa tacttcccg gataattgg gtcatttcc 
      atttacatg ggttttatc gtaaatttg gccgaaagc 
      tgggagtgc ttcaaggcg gcgaaaatg gcccttaac 
      aacaccctt gaaaccgta aacatcaaa ggttacgcc 
      agttcgtac ggaagcagc gttttgccc ctcctcaaa 
      gccacaaat ttgaagctt aaagaaggc aacatcact 
      agtgacaac ctcctcaaa gacgtcaac aacataata 
      aatcttttg agggattgc aacttcacg attcgatgg 
      ctgatgcta cacacgctc ctcaaacct ggcaaaaac 
      gacaaaagc aaaaaactg aaacaattg agagagctg 
      gttataacc gaatcgcgg ttagagtca gttcaactg 
      tttaaactg ctcctcaat acgtcacag ttggagtta 
      atcacgcgt gaaatttac aaaaacttg ttgctggaa 
      aaagacaat caatggtcg gaattgaaa agtgagagt 
      tttaagagt ttggttgag ttgagtgag gttttcagt 
      ggcgctaaa cctttgacc cgaattccg aaaaatgag 
      aatttgcaa aattggttt ttggaaatt tcgaaacaa 
      gtcgattcg ttgtgttca caagacgca gcttcgtct 
      cgtaaaatc gttcaactg attcaagca cttgaggaa 
      gtgcaggag tttcaccaa ctcgagtcc aacatgcaa 
      gtggttcag tttttgtcc gaaactcgc aaattcctg 
      caccaaatg atccgtaac atgaacata aaagaggat 
      gttttgatt acgttgcaa ataatcggc gatttgagc 
      tacgcctgg gaattgatc gattccttc actcctatc 
      atgcaattc gggattaaa aaagagcca actttggtc 
      attaaactc cgggcgatt ttcctcaaa ctctcctca 
      gcgcttgaa gtcccgctt ttgcgcatc aatcaagca 
      cacagtgag gatttgatt tcagtgtcg caatattac 
      agtagcgag cttgaaatc tatgtcagg aaggtgttg 
      caaataatt ccgaaaatg atgtttgag aaaatggct 
      cgtgtgatt gagattcaa acttgtatc ttgaaggaa 
      ttgcccaca aggctggat aaggataag ttgaaggat 
      tttgcacag ttgaatgaa cggttcgag tttgctgaa 
      ctgacgcat tcgatttcg gtttttagt caagggatg 
      agaatgatg aagagtacg ctggttgga gtgatttgt 
      ttagatccg aaacagttg ctcgaagat gggattcgg 
      aaggagttg gtgttatat atctcgaag gcgttgcat 
      acgggcttg atttttggg caaagggtt aaagttgag 
      gagttggag ggcaagttg gaggccgtg ggggttatt 
      atggatggg tataagagg tcgtttgag tatatacag 
      gattacatc aacatcaac ggtttaaaa atctggcaa 
      gaggaagtg actcgaatc atcaactac aacgtggag 
      caggaatgc aacggcttt ttgcgcaac aaaatccac 
      acttgggaa agcgctttc cagagccgt tacgtccca 
      ataccgctc tacccaagt acggactct tttagcgaa 
      aatttcatc gggcgatta gcgcgcgaa ataatcaaa 
      ttgaccgat cccaaacag tccgtttat ctcgaacaa 
      acatcgact tggtacgac atcaaaagt cacaaacca 
      atcttcaac aaagaaacc atagctcta gtagcgtct 
      gctatcgaa atttcgggc ttggtgggc cttgaccgg 
      ctcttctcc ttcatgatt atttcaaat ttgcaaaaa 
      ttgtccggg tatttggaa atgaaaaac gccaaaacg 
      aacgcatgg cataacgtt ttggcgagt attcataaa 
      gatatgatc gggagcgaa aatgtgaac aaccccatc 
      aagttttac cagaactgt gttaatcga acgacgaaa 
      atttggaac gagtttctt gattgcatt ttgttggtc 
      ggacaattg cagcttttg aggaactta atcgctttt 
      catttgaat aaatcctgc aagtttaat gcgaaaaat 
      ttagaatcg tctctacgt tcactcaat aaggctttg 
      ttactcgac attgtgaac ggcgagtgt gccccaagt 
      gaggacatc atgtccaaa ctatcgcat tatttcgat 
      tattcgggc ctgaataat cccttcaac aaaatatac 
      atcacgtca aagtttgac gataatcat tacattact 
      attttcgtt tttgcgatt gcacatttt agtaaactt 
      ttttttcta cgtcagtta ggaaacgcc aacaaacga 
      aatgcggat caaatcgac ggaaccgca tttactgtc 
      gcaatccac acccttgtc aaacaattc cataataaa 
      ttaaacgaa agtttcatc aaattatta tccaattat 
      gtcgtacag ttgactaga tatagcata agtataaaa 
      aataccgaa ttgcctttg gaggtgtca ctagctatg 
      agttttctg gaaacgtac accaattac tcagagtct 
      tcccgaaag tgtttgaaa gaagaaatt cccgatgaa 
      attttgcat ttacaacaa actctagtc gcctctgct 
      tcctag
    mRNA sequence
    • >TC000124 RA
      aacctgtta aacaactgt tatcaaaag tgaggttgt 
      ctgtgatct tttttgatg taaataaac aagattttt 
      tcacgaaaa tgccggatt ttctggcag aaaataata 
      tttgtggcc agacaattc tccagttgg tctcacgag 
      gcaatgcca tcattgccg aattgctcc gcttgaaag 
      actacatcc cccaaactt acagattgg ggagtaagg 
      aagaggtcc aaaaatacg gcgagataa tcatggact 
      ttagctatt ttaaaatcg aggaagcgc agaattaca 
      aaattgaga attcggagt tgttgcaag atttggatg 
      aggagtttc gggataatc acatcgaga tgattaatc 
      ggttttatc tcacgtttg aaagcatcc acacttata 
      ttacggatt tgaaccact ttctcgacg agttggagg 
      aagggttat acatccatc aaagtctag agacgattt 
      ttgccgaca tggagggga aacaactgc tttgtgaag 
      ctttatttt tgtacggtt taatgctgc taatcatag 
      acacgtata ttgagggcc ccatccgtg agagactcc 
      tagtgtctt actatcgct acactccgc aaagaagcg 
      acactcaaa gccaaatcg acgatgttt gccgtttat 
      tacgcgaca caggctttc acacggcga aaaaaccca 
      ataattatc ctgaggatt atttccgac gcgttcctc 
      ttaaaccaa aatacgttg acatcgtaa tcggtcatt 
      tgcgctccg acgacatct acggccaat tatcagtgt 
      acccccttc ccaaacacc gaagcaccg cactcgccc 
      accaagcct caatgcttt acgtttgtc tcttcttct 
      caccgaata ttttacatt cccaaacgg caattatgc 
      gcgaaatcg tcgataaat acttcccgg ataattggg 
      tcatttcca tttacatgg gttttatcg taaatttgg 
      ccgaaagct gggagtgct tcaaggcgg cgaaaatgg 
      cccttaaca acacccttg aaaccgtaa acatcaaag 
      gttacgcca gttcgtacg gaagcagcg ttttgcccc 
      tcctcaaag ccacaaatt tgaagctta aagaaggca 
      acatcacta gtgacaacc tcctcaaag acgtcaaca 
      acataataa atcttttga gggattgca acttcacga 
      ttcgatggc tgatgctac acacgctcc tcaaacctg 
      gcaaaaacg acaaaagca aaaaactga aacaattga 
      gagagctgg ttataaccg aatcgcggt tagagtcag 
      ttcaactgt ttaaactgc tcctcaata cgtcacagt 
      tggagttaa tcacgcgtg aaatttaca aaaacttgt 
      tgctggaaa aagacaatc aatggtcgg aattgaaaa 
      gtgagagtt ttaagagtt tggttgagt tgagtgagg 
      ttttcagtg gcgctaaac ctttgaccc gaattccga 
      aaaatgaga atttgcaaa attggtttt tggaaattt 
      cgaaacaag tcgattcgt tgtgttcac aagacgcag 
      cttcgtctc gtaaaatcg ttcaactga ttcaagcac 
      ttgaggaag tgcaggagt ttcaccaac tcgagtcca 
      acatgcaag tggttcagt ttttgtccg aaactcgca 
      aattcctgc accaaatga tccgtaaca tgaacataa 
      aagaggatg ttttgatta cgttgcaaa taatcggcg 
      atttgagct acgcctggg aattgatcg attccttca 
      ctcctatca tgcaattcg ggattaaaa aagagccaa 
      ctttggtca ttaaactcc gggcgattt tcctcaaac 
      tctcctcag cgcttgaag tcccgcttt tgcgcatca 
      atcaagcac acagtgagg atttgattt cagtgtcgc 
      aatattaca gtagcgagc ttgaaatct atgtcagga 
      aggtgttgc aaataattc cgaaaatga tgtttgaga 
      aaatggctc gtgtgattg agattcaaa cttgtatct 
      tgaaggaat tgcccacaa ggctggata aggataagt 
      tgaaggatt ttgcacagt tgaatgaac ggttcgagt 
      ttgctgaac tgacgcatt cgatttcgg tttttagtc 
      aagggatga gaatgatga agagtacgc tggttggag 
      tgatttgtt tagatccga aacagttgc tcgaagatg 
      ggattcgga aggagttgg tgttatata tctcgaagg 
      cgttgcata cgggcttga tttttgggc aaagggtta 
      aagttgagg agttggagg gcaagttgg aggccgtgg 
      gggttatta tggatgggt ataagaggt cgtttgagt 
      atatacagg attacatca acatcaacg gtttaaaaa 
      tctggcaag aggaagtga ctcgaatca tcaactaca 
      acgtggagc aggaatgca acggctttt tgcgcaaca 
      aaatccaca cttgggaaa gcgctttcc agagccgtt 
      acgtcccaa taccgctct acccaagta cggactctt 
      ttagcgaaa atttcatcg ggcgattag cgcgcgaaa 
      taatcaaat tgaccgatc ccaaacagt ccgtttatc 
      tcgaacaaa catcgactt ggtacgaca tcaaaagtc 
      acaaaccaa tcttcaaca aagaaacca tagctctag 
      tagcgtctg ctatcgaaa tttcgggct tggtgggcc 
      ttgaccggc tcttctcct tcatgatta tttcaaatt 
      tgcaaaaat tgtccgggt atttggaaa tgaaaaacg 
      ccaaaacga acgcatggc ataacgttt tggcgagta 
      ttcataaag atatgatcg ggagcgaaa atgtgaaca 
      accccatca agttttacc agaactgtg ttaatcgaa 
      cgacgaaaa tttggaacg agtttcttg attgcattt 
      tgttggtcg gacaattgc agcttttga ggaacttaa 
      tcgcttttc atttgaata aatcctgca agtttaatg 
      cgaaaaatt tagaatcgt ctctacgtt cactcaata 
      aggctttgt tactcgaca ttgtgaacg gcgagtgtg 
      ccccaagtg aggacatca tgtccaaac tatcgcatt 
      atttcgatt attcgggcc tgaataatc ccttcaaca 
      aaatataca tcacgtcaa agtttgacg ataatcatt 
      acattacta ttttcgttt ttgcgattg cacatttta 
      gtaaacttt tttttctac gtcagttag gaaacgcca 
      acaaacgaa atgcggatc aaatcgacg gaaccgcat 
      ttactgtcg caatccaca cccttgtca aacaattcc 
      ataataaat taaacgaaa gtttcatca aattattat 
      ccaattatg tcgtacagt tgactagat atagcataa 
      gtataaaaa ataccgaat tgcctttgg aggtgtcac 
      tagctatga gttttctgg aaacgtaca ccaattact 
      cagagtctt cccgaaagt gtttgaaag aagaaattc 
      ccgatgaaa ttttgcatt tacaacaaa ctctagtcg 
      cctctgctt cctagactg attatttag gggtcgcat 
      tgtgtggcg ccatctgtt attttttta ataaaatgt 
      aatttttca ctaaaaagt attacgtta ttttatgca 
      ttgattatt ttattgatg cgttccatc acagaaaaa 
      aactctttt taaaaatat taaatttga tccatcttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY