Status
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Gene information
General information
TC000119 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10911915..10950309
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC000119
      iBeetle screen iB_02979

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC000119 RA
        MDEPFGEIP KSRLSSKTE HRFDELERV DEKGKVENK 
        LICKPVQSF HRDSNVISF HIPHKKLLE KIEYKKSPP 
        EPLAGFSNI QQDLLSRKR SASEDLNIS SSDDEESII 
        SDVSRFALK YRRSTLSPS VAKEPVLPH AKLYDVMDQ 
        NEWIAKLRQ AADGYFIYM TYTVPQSSE LYSPYALSV 
        VPFPKVDKS NFFTMSSKG VMQHQGVET VFTPLDVWE 
        REFQMYCKL MHFRTFYRF RLWKGFYVW RKAVVRKKF 
        KSAQVYLNE NLFILNNEL RAGLLAIQQ MCYKMTDIS 
        FIDDDTIED FLLFYFIEN QMEKLESVS DKLQEFREV 
        AKDIVANAC HGALLAKGF VPNELLVEP SKDRKRGGV 
        VTKAQAKKT SYIEQAAKK KFCMRLTCF IILADYLMI 
        DLMHNLITR STRDLNAIL EKHVQFLPS KEKIEAATI 
        TEILEEPRP EEAPQLPLF IIDVVLNAK KLSLDPSLR 
        VTLNIFNQV MDLWQEHCK KIKSFTADE MYRIFTNPI 
        INGKEEARI CGYGPTLYF YLDDDKKLK DSIQQIVEH 
        FVHNYKMAK LYVSKYNFI REFFAEDEQ VKEEALKEE 
        RDIETFRNM CNRYHKELD IIDGVIVSQ SLGMLNLQL 
        SHLKECAKP EPERLISAL THILPWIGK NEIDRLIEE 
        SENMETSLN QQPVTTVDY VKYLEYLDD ANTKLNDME 
        NNLDYCKEL YDIMEEFAI PVPDEDMNN YLGVSVTMA 
        SLRNLVDRK LDEINKTIK SFNDQMNKD ISALISEVG 
        AIKDECMQP WLYDIESNI GEVTEFLND LYERLVTCQ 
        QRAAEFKGY QKQFRLEVT RFDILDEVM ADVKLRMLL 
        WESVTSWSK TVDEWYHSD FSELNVEDM NLYTAKTIK 
        NINLLEKGL PKNLIVPKL KDDVELMKT KLPVVAYLR 
        NPSLRQRHW IQVENILSY KFKPDDEVT LELLENLKV 
        FSFANELQE ISGQASSEA SLEQLLKKV EEAWKTLEF 
        QVMLHKDSK DVYILGSLE EVQSVLDDS TINITTIAS 
        SRHVGPIKS RVEEWARQL DLFSRTLDE WVACQQSWL 
        YLEVIFSAP DIQRQLPSE SKLFIIVDK SWKQIMRRT 
        AKMPLAIEA AFYPDLLEE LQKNNALLE QIMKCLESY 
        LEVKRVAFP RFYFLSNDE LLEILAQTR NPHAVQPHL 
        RKCFDAIAR LEFAAKEEE VGITPGGDE DVKKSKSVT 
        MLTTNISAM ISPEGERVA LTKGLKARG NVEDWLGKV 
        ESSMFFALR RLMKGALID FQQSRRIEW VVRHPSQIT 
        LTVSQIMWA RGVHAILDH GNAHSNLEK FEQKCIGDL 
        NDLATLIRS DLDSVTRKV LIALITIDV HARDTIRNM 
        VEHGVKKSD SFDWLKVLR YYWDEHLDD CVTRMSSAY 
        YIYGYEYLG ASGVLVITP LTDRCYLCL MGALQLDLG 
        GAPAGPAGT GKTETTKDL AKALAIQCV VFNCSEGLD 
        YKIMGRFFA GLAQSGAWS CFDEFNRID IEVLSVIAQ 
        QIITIRNAK AAKLTRFMF EGREIKLVQ KCATFITMN 
        PGYAGRTEL PDNLKALFR PIAMMVPDY ALIAEVILY 
        SEGFESSKV LSQKMVQMY KLCSEQLSQ QDHYDFGMR 
        AVKSVLVMA GSLKRANPD RNEDVVLIC ALRDSNLPK 
        FLADDALLF QGILSDLFP GVELPEQDY GKFQEAIVD 
        VMVHQQLQP EKPMIRKVI QLYETMIVR WGVMLVGPT 
        GGGKSTVLN TLNKALTKM YNDGIEGPY YHPVHTYTM 
        NPKAVTAGE LYGEVNIYT LEWRDGLMG IMMRTAVQC 
        TEEDHQWII CDGPVDAVW IENLNTVLD DNKMLCLAN 
        SERIKLTPY VHMVFEVMD LAQASPATV SRCGMVYID 
        PDEIGWLPY AISWVQRRD EELLNHELK QFMIGLFEY 
        AVENGFAFV KKNGDYSIH QVDISKVAM LCAIIESYL 
        NSPNALENI GEKSKVKSY LCQVFIFAY IWSLGGNLT 
        DASKEKFEV YVREQFDDH PDARLPPGV DLYGVFMNT 
        QDHRLDPWA KILPTFTYK QEVPFFEML VPTNDTVRF 
        GYVMERLMY VNYPVLFVG DTGVGKTVV AKDVLNRLY 
        ETGQFVPVT INFSAQTSS FRTQEILEL KLEKKKKTL 
        LGAPLGKKV LLFVDDVNM PKLETYGAQ PPIELLRQF 
        LTYGGLYDR EKLFWKEIR DVIVSAACA PPGGGRNPL 
        TPRFVRFFA MLLIPPPNE FSLKAIFKA ILKGFFFDF 
        SNEIRDLAD YMVGAAVEI YMRIATDLL PTPAKSHYV 
        FNLRDLSKC VQGVLQADS GTMREESAM LRLFYHECL 
        RVFHDRLIN VEDKSYFYF LMREICGRN FGTAVLALP 
        DQPVITNPP LLLFGDFMQ YGANKEDRI YEEIRNVDK 
        IRSVLQDYL DDYNLLESK DMRLIFFMD AIEHCVRIA 
        RILRSERGN ALLVGVGGM GKQSLTRLA SHVNAYKCF 
        QIELTRNYD RSYFFEDLR KMYFNAGAN NANSVFLFT 
        DTQIVQEDF LEDINNILN SGEVPNLFE ADEYEKVII 
        ATRDPAKGA GVDPANRDG IYDYFISRV RNNLHLVIC 
        MSPVGDAFR RRCRMFPSL VNCCTIDWF EKWPHEALL 
        SVSQNALKD LGSEELCHN LSTICVTIH ESVEEMTER 
        FYLEMRRHY YTTPSSYLE LLKLYRKLL ETKKEQVIY 
        KRSRISNGL QKLYETNSV IETMKETLI ELEPVLAEK 
        SVAVDELMS DLTTEQHQA DKVRAIVKY DEEIAKAKA 
        EDTQALADD AQRDLDTAM PALEAATKA LEALNKNDI 
        NEIKVFQKP PKLVQYVME SVCLLLGAK TDWASAKIV 
        LGDVNFLKK LQEYDKNHI TEQTLRKLK SYVDNPDFV 
        PDKVGRVSK ACKSMCMWV RAMDMYAKV YKIVEPKRK 
        RLEQAEKEL NQVMGLLRE KQRQLAEVE AMIASLEAK 
        FNQTLAEKD ALENEMELT SNRLNRAGR LNVALGDEQ 
        ARWERSVKE FAVELQNII GDALIAAAC VAYLGAFTS 
        LYRNELVDL WVSQFKEFQ IPASDNFSL IRVLADPYD 
        IRMWNSFGL PRDTVSTEN AILVTQASR WALMIDPQE 
        QANRWIRQM EAANDLRVV KLTDSNFLR VLESAIRIG 
        KPVLLEEVG ETLDPTLGP ILTKQTFMQ AGRLLIRLG 
        DSDVEYDPN FRFYVTTKL ANPHYLPEI CIQVTIVNF 
        TVTKSGLED QLLADVVRL ERPDLESQR TELIIRINN 
        DKTQLQLIE DKILKLLYQ SEGNILDDE ELIETLNES 
        KETSAVIAA RLLETESTE RKISEAREK YRTVSIRGS 
        VLYFVIAQL AEIDPMYQY SLKYFNQIF NTVIETSEK 
        SNDLKERLK ILLREITAF VYTNVSRGL FERHKLVFS 
        FMLCVAIHQ EKGEISDNQ WNFLLRGPV GAKIELPPK 
        PDYPLITDA MWLSANFLA VSIPGFEKL PEEITNVIS 
        VRISDFQQD ISVVPNAKS SRVKWNELL DDFNKLLLL 
        KTLKEEKLV FAITEYVKI KLGKQFIES PQVSLQVLY 
        QDTSNITPL VFILSTGSD PFGSFQRFA DEMGFRERI 
        KSISLGQGQ GPVAEKIIE QGLERGDWV FLQNCHLAT 
        SWMLAMERL VIKIAEQSS KVHKEFRLY MSSMPSKSF 
        PVSVLQNSV KVTNEPPKG IRANIKRAF TDMQHDFFE 
        DHPLKQDWR CMIFGTCMF HAIIQERKK FGPLGWNIT 
        YEFNDSDRE FAFNTLKMF CAEGTIPWD ALEYLTGEI 
        TYGGRVTDY WDLRCLKTI LKIFFSPQI LKPHYKYSP 
        SGIYYCPSY SKLEKYQEF IDGLPILEA PEIFGMHEN 
        ANIAYQIKE TQNILLTIM ESQPHTSGG AEGQQTDNI 
        VYDLANLVT DSLMTKIST DEANVNMFK RDDKGRLPS 
        LTTVLMQEV DRYNILLKL IHSSMDNLK KAIKGLVVM 
        SDALEEVYV AFTNNQVPK MWNKKAYNS LKSLGSWIR 
        DLVLRLDFI KIWVRSGPP SSYWLSGFY FPQGFLTGT 
        LQTHARKYN LPIDQLKFD FDVQKVWIE QEQVKKIHD 
        EEQHENLEV YKGLHHPED GAIIHGLFL DAGRWDSPT 
        HKLVDAKPG EINPLLPAI WMLPKTSLP PNDKRYVTP 
        LYKTSIRAG VLSTTGHST NFVIAVLLP TDKPQSYWI 
        LKGTALLTQ VTD
      CDS sequence
      • >TC000119 RA
        atggatgag ccatttggt gaaatcccg aaatccagg 
        ctgagttcg aaaactgaa cacaggttt gatgagctc 
        gagcgggtc gacgagaag ggcaaagtc gaaaataag 
        ctcatttgc aagcccgtt cagtcgttc cacagagat 
        tccaatgtc atttcattt cacattcca cacaagaaa 
        cttctcgaa aaaatcgaa tataagaaa tcgccaccc 
        gaacctttg gccggtttc tcaaacatc caacaagac 
        ttattgagt cgcaaacga agcgcttct gaagacctt 
        aatatcagt tcttcggac gacgaagaa tcaataatc 
        agtgatgtt tcacgattt gctttaaaa taccgacgg 
        agtactctt tcgccgtct gtggcaaaa gaacccgtc 
        cttcctcac gccaaactt tacgatgtc atggatcaa 
        aacgaatgg atcgcaaaa ttacgacaa gccgccgat 
        ggttatttc atttatatg acttacacg gtaccacaa 
        tcttcagaa ctttactcc ccttacgcc cttagcgtt 
        gtgcctttt ccaaaagta gacaagagt aattttttc 
        acaatgagt tccaaaggt gtgatgcaa caccaaggg 
        gttgaaacg gtgttcacc cctctggac gtgtgggaa 
        cgcgagttt caaatgtac tgcaaactg atgcacttc 
        cgcacattt tatcgtttc cgattgtgg aaaggtttc 
        tacgtttgg cggaaagct gtagttcgt aaaaaattc 
        aaatcggca caagtttac ctcaacgaa aacttgttt 
        attttaaat aatgaactg cgagcgggt ctcttggcc 
        atccaacaa atgtgctac aaaatgacg gatatttcc 
        ttcattgac gacgacacc atcgaggat tttctccta 
        ttttatttc attgaaaat cagatggaa aaactcgaa 
        tcagtgtcg gacaaactg caagaattt cgcgaagtg 
        gccaaagat attgtcgcg aatgcgtgc catggggct 
        cttttagcc aaaggtttc gtcccaaat gagttactc 
        gttgagcca agcaaagac cgtaaacgt ggtggtgtt 
        gtgaccaaa gctcaggcc aagaaaacc tcgtacatt 
        gagcaagcg gccaagaaa aaattctgt atgcgtctc 
        acgtgtttt attattctt gccgattat ttaatgatt 
        gatttgatg cacaatttg attacgcgg tcgacgcgc 
        gatcttaat gccattttg gaaaaacac gttcagttt 
        ttgccctcg aaggaaaaa atcgaggct gcgaccata 
        acggagatt ttggaggag ccaagacca gaggaggct 
        cctcagctt cctttattt atcattgat gttgttttg 
        aatgcgaaa aaattgtcg ctggatccg tctctcaga 
        gtcactttg aacattttc aatcaagtg atggatttg 
        tggcaggag cattgtaaa aaaattaag tcgtttacg 
        gccgatgaa atgtatcgg attttcaca aatccgata 
        ataaacggt aaagaggag gctcgtatt tgtggctat 
        ggccccact ttatatttc tatttagac gacgataaa 
        aaattgaaa gattccata caacaaatc gttgaacat 
        ttcgtccat aattataaa atggccaag ctctacgtt 
        agtaaatat aatttcatt cgggaattt ttcgccgaa 
        gacgagcaa gtcaaggaa gaagccttg aaggaggaa 
        agagatatt gaaacgttt cgaaatatg tgtaaccgc 
        taccataaa gaactcgac attattgac ggtgttatc 
        gtatcgcaa tctcttgga atgttaaat ttgcaactg 
        agccatttg aaagaatgt gcaaaaccg gaaccggaa 
        cgcctcatt tcggccctt acacacata cttccttgg 
        attggtaaa aacgaaatt gatcgtttg attgaagaa 
        tcggagaat atggagacg agtctcaac caacaaccc 
        gtcacaaca gttgactat gtcaagtat ttggaatat 
        ttggacgat gctaatacc aagctgaac gatatggag 
        aataatctg gactattgt aaagaattg tatgacatt 
        atggaggaa tttgcgatc ccggtgccc gacgaagac 
        atgaacaat tatctaggt gttagcgta acgatggct 
        tctttgcgc aatttggtc gaccgaaag ttggacgag 
        attaataaa accatcaag agttttaac gatcagatg 
        aacaaggat atcagtgct ttgatttcg gaagtggga 
        gccataaaa gacgaatgc atgcaacct tggttgtac 
        gatattgag tcaaatatc ggcgaagtg accgaattt 
        ttgaacgat ttgtacgaa cgacttgtc acttgtcag 
        cagagagcg gctgaattt aaaggctac cagaaacaa 
        tttcggttg gaagtgaca cgttttgat attttggac 
        gaagttatg gcagacgtg aagttacgt atgctcctg 
        tgggagagt gtcacctcc tggagcaaa accgtggac 
        gagtggtac cactctgat ttcagcgaa ttgaacgtc 
        gaagacatg aacttgtac acggcgaaa actatcaaa 
        aatatcaat cttctggag aagggattg cccaaaaat 
        ttgatcgtt ccgaaacta aaggacgat gtcgagttg 
        atgaagacc aagttgcca gtcgttgca tatttgcgg 
        aacccaagt ctacgacaa cgccactgg atccaagtg 
        gagaacatc ctcagctac aaattcaag ccggacgat 
        gaagtcaca cttgagctt ttggaaaat ttgaaagtt 
        ttcagtttt gcgaatgaa cttcaggag atttcgggt 
        caggcgagt agtgaggcg agtctcgag cagcttttg 
        aagaaggtg gaagaagcg tggaagacg ctcgagttt 
        caagttatg ttgcacaag gattcgaag gatgtttat 
        attttgggt tcgctggag gaggttcag agtgttttg 
        gatgatagt accataaat ataacgaca attgcgagt 
        tcgagacat gtgggtccg attaaatca cgtgtggag 
        gagtgggcc cgacagttg gatttgttt tcgaggacg 
        ctggatgaa tgggtggct tgtcaacag agctggctt 
        tatttggag gtgattttt tccgcgcct gatattcaa 
        agacagctt ccgagcgag tcgaagctt tttatcatt 
        gtggacaag tcgtggaag cagattatg cgacggacc 
        gcaaagatg cccctagct attgaagcc gctttctat 
        cccgacttg ctcgaagag ctccaaaaa aacaacgca 
        cttttggaa caaatcatg aagtgtctc gaatcctac 
        ttggaagtg aaacgtgtg gcattccct cgtttctac 
        ttcctatca aacgacgaa cttttggag attttggcc 
        caaacccga aacccacac gccgtccaa ccccacttg 
        cgtaaatgt ttcgacgca attgctcgt ctagaattt 
        gcagcaaaa gaggaagaa gtcggcata acacctggc 
        ggtgacgaa gatgtgaaa aaatccaaa tctgtgacg 
        atgctcacg acaaatatt tcggcaatg atcagccct 
        gaaggcgaa cgcgtggca ctgactaag ggcttgaaa 
        gcgcgagga aacgtcgag gattggctg ggaaaagtg 
        gaatcgtct atgtttttc gctctgaga cgtttgatg 
        aaaggggca ctgatcgat ttccagcaa agtcggaga 
        attgaatgg gtggtgaga cacccgagt cagatcacc 
        ttgactgtg tcgcaaatc atgtgggcc agaggtgtc 
        cacgcgatt ttggaccac ggaaacgcc cacagtaat 
        ttggaaaag tttgagcag aaatgtatc ggagatttg 
        aacgatttg gccactttg attcgctcg gatttggat 
        tcagtgact cgaaaagtc ttgattgcg ttaattacg 
        atcgatgtc catgccaga gataccatc aggaacatg 
        gtcgagcac ggagtcaaa aaaagcgat agtttcgac 
        tggttgaaa gtcctgagg tactactgg gacgaacat 
        cttgacgat tgcgttaca agaatgtct tcggcttac 
        tacatttac ggttacgag tatttgggg gcgtctggt 
        gtcctggtt ataaccccc ttaaccgac cgctgctac 
        ctctgtttg atgggtgcc ctccagctg gacttaggg 
        ggcgctcct gcaggtcca gcgggaact ggcaaaacc 
        gaaaccact aaagatttg gccaaagct ttggccatt 
        cagtgcgtg gtgtttaac tgttcggaa ggtttggat 
        tataagatt atgggtcgg ttttttgcc ggattggca 
        cagtccggt gcttggagt tgtttcgat gagtttaat 
        cgaatcgat attgaggtg ctttcggtg attgcgcaa 
        caaattatc accatacgg aacgccaaa gctgcgaaa 
        ttgacacgg ttcatgttt gagggacgc gaaattaag 
        ctggtgcag aagtgtgcc acttttatt acgatgaat 
        ccgggatat gccggaagg actgaactt ccggataat 
        ctgaaggct ttgtttagg cctattgcg atgatggtc 
        cccgattac gctctcatc gccgaagtg attttgtat 
        tcggaaggt ttcgaatcg tcaaaagtt ctctctcaa 
        aaaatggtg caaatgtat aagctttgt agtgagcaa 
        ttgtcccaa caggatcac tatgatttt gggatgcgt 
        gccgttaag agtgtcttg gtgatggca ggctccctg 
        aagagggca aaccccgac cgaaacgag gacgtggtg 
        ttaatttgt gcgttgcgc gacagtaat ctaccgaaa 
        tttttggcc gacgatgct ctccttttc caaggaatt 
        ttgagcgac ttgtttccc ggagttgag ttaccggaa 
        caggattac ggtaagttc caggaggcc atcgtggac 
        gtcatggtg caccaacag ctccaaccg gaaaaaccc 
        atgattcga aaagtgatt caattgtac gaaaccatg 
        attgttcga tggggcgtg atgttggtg gggcccaca 
        gggggtggg aaaagcaca gttttaaac actttgaat 
        aaagccttg acgaaaatg tacaatgat ggtatcgaa 
        gggccttat tatcaccca gttcacact tataccatg 
        aacccaaag gctgtaacc gctggtgag ttgtacgga 
        gaggtaaac atttatacg ctggaatgg cgcgatggt 
        ctcatgggt atcatgatg cgaactgct gtgcaatgt 
        acggaagaa gaccaccaa tggattatt tgtgacggt 
        ccagtggat gctgtttgg attgaaaac ttaaacacc 
        gttttggac gacaataaa atgttgtgt cttgcaaat 
        tcggagcgt attaaactc acaccttac gtccatatg 
        gtgttcgaa gtgatggat ctggcacag gcttctcca 
        gctactgtc agtcgttgt ggtatggtc tatatagac 
        cctgatgaa attggctgg ttgccttat gccatatcg 
        tgggtgcaa agacgcgac gaagagctt cttaaccac 
        gaattgaaa cagtttatg attggtttg tttgaatat 
        gctgttgaa aatggtttt gcttttgtg aaaaaaaat 
        ggagattac tctatacac caagtggat ataagtaaa 
        gttgcaatg ctttgtgcc atcattgag agttatttg 
        aattcaccg aatgcgttg gaaaatatc ggcgaaaag 
        agcaaagtt aagagctac ttgtgtcaa gtgtttatt 
        ttcgcctat atttggtca cttggcggg aatcttacc 
        gacgcgtca aaggaaaag tttgaagtt tatgtgagg 
        gaacagttt gatgaccat ccagatgca aggttgcct 
        ccaggagtc gacctctac ggcgtattt atgaacacg 
        caagaccac cgcttggac ccatgggca aaaatcctc 
        ccaaccttc acctacaaa caagaagta ccatttttt 
        gaaatgttg gtaccaacc aacgacact gtccgattt 
        ggttacgtc atggaacgt ctaatgtac gtaaattat 
        cctgtacta ttcgtcgga gatactggt gttggcaaa 
        acagtcgtt gcaaaagac gtccttaac cgtttatat 
        gaaacgggt caatttgtt ccggtgact ataaatttt 
        tcggctcaa actagcagt tttcgaaca caggaaatt 
        ctcgaattg aagcttgaa aaaaagaaa aaaactctt 
        cttggtgcc ccattgggc aaaaaagtg ttacttttc 
        gtcgatgat gtcaatatg cccaaattg gagacatac 
        ggggcccaa cccccgatt gaattacta cgacagttt 
        ctaacctat ggtggtttg tacgatcgg gagaaatta 
        ttttggaag gaaattcgc gatgtgatt gtgtcggcg 
        gcttgtgca ccccctggg ggcggcagg aacccgctc 
        acgccccga tttgtgcga tttttcgca atgttacta 
        atcccacca ccgaatgag ttctccctc aaggcgatt 
        tttaaggcg attttaaaa ggttttttc tttgatttt 
        tcgaacgaa atcagagat ttggccgac tatatggtg 
        ggggctgcc gttgaaatt tacatgcgg attgcgacc 
        gatttgttg ccaactcct gcaaaatct cactacgtg 
        ttcaatcta cgggattta tcgaaatgt gtgcaaggg 
        gtgctccag gcggattct ggcaccatg agggaggaa 
        agtgcaatg ttgcgtctg ttctaccac gagtgtttg 
        cgcgttttt cacgatcga ttgatcaat gttgaggat 
        aaatcgtac ttttacttt ctgatgagg gagatttgt 
        ggtagaaat ttcggtact gcggttcta gccttgcca 
        gatcagccg gttataacc aatccgcct ttgctactt 
        ttcggcgac tttatgcaa tacggcgcc aataaagag 
        gaccggatt tatgaggaa attaggaac gttgacaag 
        attcgaagc gttttacaa gattatttg gacgattat 
        aatcttctc gaatcgaaa gacatgcgg ttgatattt 
        ttcatggac gcgattgaa cattgtgtt cgtatcgct 
        cgaatattg cgatctgag aggggaaat gccctcctt 
        gtgggggtt ggaggaatg gggaagcaa agtttgacg 
        cgattagct tcacacgtg aatgcttac aagtgtttt 
        caaattgaa ctgacgcga aactatgac cggtcgtac 
        ttttttgag gatttgaga aaaatgtat tttaatgct 
        ggagcgaat aatgcaaat tcggttttc ctgtttacc 
        gatacgcaa atcgtgcaa gaagatttt ttggaagat 
        attaacaat attctaaat tcgggcgaa gtgccgaat 
        ctttttgag gccgacgaa tacgaaaaa gtgataata 
        gctaccaga gatccggca aaaggtgca ggagtcgat 
        cctgcaaac cgtgacggt atttacgac tattttata 
        agcagagtt cgcaataat ttgcatttg gtgatctgt 
        atgagtcca gtgggtgac gcttttagg cgccgttgt 
        cgcatgttt ccctctttg gtcaattgt tgtacaatt 
        gattggttc gaaaaatgg ccacatgaa gccctatta 
        tctgtgtct caaaatgct ttaaaagac ctgggaagt 
        gaggaactt tgccataac ttatcgacg atttgtgtg 
        acgatacac gaaagtgtg gaagaaatg actgaacgg 
        ttttatctc gaaatgaga cgacattat tacacgaca 
        ccgagtagc tacctcgaa ttgttgaaa ctgtaccga 
        aaattactc gaaacgaaa aaggaacaa gttatttac 
        aaacgatcc agaattagt aacggatta caaaaattg 
        tacgaaacg aattcagtt atcgaaaca atgaaagaa 
        actctgatt gagctggaa ccagttttg gccgaaaaa 
        tcggtcgct gtggacgaa ctaatgtca gatttgact 
        acggaacaa caccaagcc gacaaagtc cgagctatt 
        gtcaaatac gacgaggaa attgccaaa gcaaaagcc 
        gaagatact caagcattg gctgacgat gctcaaaga 
        gacctggat acggcaatg ccggctttg gaagctgca 
        accaaagct cttgaagca ctcaacaaa aacgatatc 
        aacgaaatt aaagtgttt caaaagccg ccgaaactt 
        gtacagtat gttatggaa tcggtttgt ctgctatta 
        ggtgccaaa actgactgg gcctcagct aagattgtt 
        ctcggcgat gttaatttc ttaaagaag cttcaggag 
        tacgacaag aatcacata actgaacaa actctacgt 
        aagttgaag tcgtacgtt gataatcca gattttgtt 
        cctgataaa gtcgggaga gtgtcaaaa gcgtgcaaa 
        agcatgtgc atgtgggtg cgtgcgatg gatatgtat 
        gcaaaagtt tacaaaatt gtggagccg aagagaaag 
        agattggaa caagccgaa aaagagctc aaccaagtg 
        atgggcctt ttgcgcgag aaacagcgc caactcgcc 
        gaagtcgaa gcaatgatc gcttcactc gaagccaaa 
        ttcaaccaa actctggcg gaaaaagac gccctcgag 
        aacgaaatg gaattaacc tccaacaga ttgaaccgt 
        gccggccgt ttaaacgtt gctctcggc gacgaacaa 
        gctcgatgg gagcgttca gtcaaagag ttcgccgta 
        gaactccaa aacatcatc ggcgatgct ttaattgcc 
        gctgcttgt gtggcctat ttgggcgct tttaccagc 
        ttgtatcgc aacgaattg gtcgatcta tgggtctcg 
        caatttaaa gagttccaa ataccggct tcggataat 
        ttcagtttg attcgagtt ttggccgat ccgtacgat 
        attcgcatg tggaactct ttcggacta ccccgtgac 
        actgtttcg actgaaaat gcaattttg gtcactcag 
        gctagtcgc tgggccctt atgattgat ccgcaggaa 
        caagccaat cgttggatt cgtcaaatg gaagctgcg 
        aacgatttg cgagtggtg aaactgacc gattcgaat 
        tttttgcgc gttttggag agcgcgatt cgaattggt 
        aaaccggtg cttttggag gaggtgggc gagacgttg 
        gatccgact ttgggcccg attttgacc aaacagacg 
        tttatgcag gctggacgg cttttgata cgtttgggg 
        gattcggat gttgagtat gatccgaat tttcgattt 
        tatgttaca acgaaattg gcgaatccg cattatttg 
        cccgaaatt tgtattcaa gtgacgatt gttaatttt 
        acggtgacc aagtcgggg ttggaggat cagttgtta 
        gctgatgtt gtgcgttta gaacgaccc gatttagag 
        tcacaaaga acagaactg attatacga atcaataac 
        gacaaaaca cagttgcaa ttaatcgaa gataaaatt 
        ttgaaattg ttgtatcag tccgaaggt aatattttg 
        gacgatgaa gaattaatt gagactcta aatgagagc 
        aaagaaact tcagctgtg attgcggct cgtctttta 
        gagaccgaa tcaacggaa cgtaaaatt tcagaagcg 
        cgcgaaaaa taccgaact gtgtccatc aggggctca 
        gttctttat ttcgtcatt gcacaactg gctgaaatt 
        gacccaatg taccaatac tcactgaaa tatttcaac 
        caaatcttc aacacagtg attgaaacc agcgaaaaa 
        agcaacgat cttaaagaa cggctcaag attttgttg 
        cgcgaaatc accgcattt gtctacaca aacgtttca 
        aggggttta tttgagcga cacaaactg gttttcagt 
        tttatgctt tgcgttgcg attcaccaa gaaaaggga 
        gaaattagc gacaaccaa tggaatttt ttgctcaga 
        ggtccagtc ggggccaaa attgaattg cccccaaaa 
        ccggattat cccctaatt acggacgct atgtggctt 
        tcggccaat ttcttggcc gtttcgatt ccaggcttt 
        gagaaattg ccagaagag atcacaaat gtgataagt 
        gtcagaatt tctgatttc cagcaggat atttcggtg 
        gtgccaaat gccaagtcg agtcgagtc aaatggaac 
        gagcttttg gacgatttt aacaagttg ttgcttttg 
        aaaaccttg aaagaggag aaattggtg tttgccatc 
        accgaatat gttaaaatc aaactgggg aaacaattt 
        atcgaaagt ccgcaagtc tcgcttcaa gttttgtat 
        caggacacg tcaaatata actcctttg gtgtttatc 
        ctgagcacg ggttcagat ccgtttggg tcgttccaa 
        cgttttgcc gatgaaatg ggttttagg gaaaggatc 
        aagtcgatt tcgcttgga caaggacaa ggtcctgtt 
        gctgaaaag ataatcgaa caaggattg gaacgtggc 
        gattgggtc tttctccaa aattgtcat ttggcaacg 
        tcttggatg ttggcaatg gaacgcctg gttataaaa 
        attgccgaa cagtcgtcc aaagtccac aaggaattc 
        cgcctctac atgagttca atgccatcg aagtcgttt 
        cccgtttca gttttgcaa aattcggtc aaagtgacc 
        aacgagcca ccgaaaggg attagggcc aatattaaa 
        agggccttc accgatatg caacatgac tttttcgaa 
        gatcatcct ttaaagcaa gactggaga tgcatgatt 
        ttcggtact tgcatgttt catgcaata atacaggaa 
        cgaaaaaag tttggccct ttggggtgg aacatcact 
        tatgagttt aacgacagc gatcgcgaa tttgcgttc 
        aatactttg aaaatgttt tgtgctgaa gggacgatc 
        ccttgggac gctttagag tatttaaca ggggagatt 
        acctacggt ggacgtgtt actgattat tgggacttg 
        aggtgtttg aaaacgatt ttgaaaatc ttcttctcg 
        cctcaaata cttaaaccg cactataag tactctcct 
        agtgggatt tattactgc cctagctac tctaaactt 
        gagaaatat caagagttt atcgacggg cttccgatt 
        ttggaagca ccggagatt ttcggaatg catgaaaat 
        gcgaacatt gcctatcaa atcaaagag actcagaac 
        atacttttg acgattatg gaaagtcaa ccgcacact 
        tctggcggt gctgaaggg caacaaacc gataacatt 
        gtctacgat ttggcgaat ttggtcact gattctttg 
        atgacgaaa atctcgact gacgaggcc aacgtcaac 
        atgtttaaa agagacgac aagggccgt ttgccctca 
        ctcacaaca gtcctaatg caagaagtc gaccgttac 
        aacatttta ctcaaactg atacacagt tcgatggat 
        aatttgaag aaagcaatc aaaggtttg gtcgtaatg 
        tcggacgct ttggaggag gtctacgtt gcatttacc 
        aacaatcag gtgccaaaa atgtggaac aaaaaagcc 
        tacaattcg ttaaaaagt ctcggaagt tggatacga 
        gacttggtg ttgcggctc gattttatt aaaatttgg 
        gtacgatcg ggtccaccg tcttcctat tggttatca 
        gggttttat ttcccacaa ggtttctta acggggact 
        ttacaaacc catgccaga aagtataat ttaccgatc 
        gatcaactc aaatttgat tttgacgta caaaaagtg 
        tggattgag caggagcaa gtgaagaaa attcatgat 
        gaggaacaa catgaaaat ttggaagtt tataaaggc 
        ttgcatcat ccggaagat ggggctata attcatggt 
        ctgttcctg gatgcaggt cgctgggat tccccgact 
        cataaattg gttgatgct aaaccaggt gaaattaat 
        ccattgttg ccggccatt tggatgtta ccaaagact 
        tcgttacct cctaatgat aagagatat gtcactccg 
        ttatacaaa acttcaata cgtgcaggt gtcttgtcc 
        acaacaggg cacagcaca aattttgtg attgctgtc 
        ttgttacca accgataaa ccacaaagt tattggatt 
        cttaaagga actgcatta ttaacacaa gtcactgat 
        tga
      mRNA sequence
      • >TC000119 RA
        tgaaaataa aattcgtgt atctgtgtg aagtgaata 
        caaaactgt taatttttc gaaatggat gagccattt 
        ggtgaaatc ccgaaatcc aggctgagt tcgaaaact 
        gaacacagg tttgatgag ctcgagcgg gtcgacgag 
        aagggcaaa gtcgaaaat aagctcatt tgcaagccc 
        gttcagtcg ttccacaga gattccaat gtcatttca 
        tttcacatt ccacacaag aaacttctc gaaaaaatc 
        gaatataag aaatcgcca cccgaacct ttggccggt 
        ttctcaaac atccaacaa gacttattg agtcgcaaa 
        cgaagcgct tctgaagac cttaatatc agttcttcg 
        gacgacgaa gaatcaata atcagtgat gtttcacga 
        tttgcttta aaataccga cggagtact ctttcgccg 
        tctgtggca aaagaaccc gtccttcct cacgccaaa 
        ctttacgat gtcatggat caaaacgaa tggatcgca 
        aaattacga caagccgcc gatggttat ttcatttat 
        atgacttac acggtacca caatcttca gaactttac 
        tccccttac gcccttagc gttgtgcct tttccaaaa 
        gtagacaag agtaatttt ttcacaatg agttccaaa 
        ggtgtgatg caacaccaa ggggttgaa acggtgttc 
        acccctctg gacgtgtgg gaacgcgag tttcaaatg 
        tactgcaaa ctgatgcac ttccgcaca ttttatcgt 
        ttccgattg tggaaaggt ttctacgtt tggcggaaa 
        gctgtagtt cgtaaaaaa ttcaaatcg gcacaagtt 
        tacctcaac gaaaacttg tttatttta aataatgaa 
        ctgcgagcg ggtctcttg gccatccaa caaatgtgc 
        tacaaaatg acggatatt tccttcatt gacgacgac 
        accatcgag gattttctc ctattttat ttcattgaa 
        aatcagatg gaaaaactc gaatcagtg tcggacaaa 
        ctgcaagaa tttcgcgaa gtggccaaa gatattgtc 
        gcgaatgcg tgccatggg gctctttta gccaaaggt 
        ttcgtccca aatgagtta ctcgttgag ccaagcaaa 
        gaccgtaaa cgtggtggt gttgtgacc aaagctcag 
        gccaagaaa acctcgtac attgagcaa gcggccaag 
        aaaaaattc tgtatgcgt ctcacgtgt tttattatt 
        cttgccgat tatttaatg attgatttg atgcacaat 
        ttgattacg cggtcgacg cgcgatctt aatgccatt 
        ttggaaaaa cacgttcag tttttgccc tcgaaggaa 
        aaaatcgag gctgcgacc ataacggag attttggag 
        gagccaaga ccagaggag gctcctcag cttccttta 
        tttatcatt gatgttgtt ttgaatgcg aaaaaattg 
        tcgctggat ccgtctctc agagtcact ttgaacatt 
        ttcaatcaa gtgatggat ttgtggcag gagcattgt 
        aaaaaaatt aagtcgttt acggccgat gaaatgtat 
        cggattttc acaaatccg ataataaac ggtaaagag 
        gaggctcgt atttgtggc tatggcccc actttatat 
        ttctattta gacgacgat aaaaaattg aaagattcc 
        atacaacaa atcgttgaa catttcgtc cataattat 
        aaaatggcc aagctctac gttagtaaa tataatttc 
        attcgggaa tttttcgcc gaagacgag caagtcaag 
        gaagaagcc ttgaaggag gaaagagat attgaaacg 
        tttcgaaat atgtgtaac cgctaccat aaagaactc 
        gacattatt gacggtgtt atcgtatcg caatctctt 
        ggaatgtta aatttgcaa ctgagccat ttgaaagaa 
        tgtgcaaaa ccggaaccg gaacgcctc atttcggcc 
        cttacacac atacttcct tggattggt aaaaacgaa 
        attgatcgt ttgattgaa gaatcggag aatatggag 
        acgagtctc aaccaacaa cccgtcaca acagttgac 
        tatgtcaag tatttggaa tatttggac gatgctaat 
        accaagctg aacgatatg gagaataat ctggactat 
        tgtaaagaa ttgtatgac attatggag gaatttgcg 
        atcccggtg cccgacgaa gacatgaac aattatcta 
        ggtgttagc gtaacgatg gcttctttg cgcaatttg 
        gtcgaccga aagttggac gagattaat aaaaccatc 
        aagagtttt aacgatcag atgaacaag gatatcagt 
        gctttgatt tcggaagtg ggagccata aaagacgaa 
        tgcatgcaa ccttggttg tacgatatt gagtcaaat 
        atcggcgaa gtgaccgaa tttttgaac gatttgtac 
        gaacgactt gtcacttgt cagcagaga gcggctgaa 
        tttaaaggc taccagaaa caatttcgg ttggaagtg 
        acacgtttt gatattttg gacgaagtt atggcagac 
        gtgaagtta cgtatgctc ctgtgggag agtgtcacc 
        tcctggagc aaaaccgtg gacgagtgg taccactct 
        gatttcagc gaattgaac gtcgaagac atgaacttg 
        tacacggcg aaaactatc aaaaatatc aatcttctg 
        gagaaggga ttgcccaaa aatttgatc gttccgaaa 
        ctaaaggac gatgtcgag ttgatgaag accaagttg 
        ccagtcgtt gcatatttg cggaaccca agtctacga 
        caacgccac tggatccaa gtggagaac atcctcagc 
        tacaaattc aagccggac gatgaagtc acacttgag 
        cttttggaa aatttgaaa gttttcagt tttgcgaat 
        gaacttcag gagatttcg ggtcaggcg agtagtgag 
        gcgagtctc gagcagctt ttgaagaag gtggaagaa 
        gcgtggaag acgctcgag tttcaagtt atgttgcac 
        aaggattcg aaggatgtt tatattttg ggttcgctg 
        gaggaggtt cagagtgtt ttggatgat agtaccata 
        aatataacg acaattgcg agttcgaga catgtgggt 
        ccgattaaa tcacgtgtg gaggagtgg gcccgacag 
        ttggatttg ttttcgagg acgctggat gaatgggtg 
        gcttgtcaa cagagctgg ctttatttg gaggtgatt 
        ttttccgcg cctgatatt caaagacag cttccgagc 
        gagtcgaag ctttttatc attgtggac aagtcgtgg 
        aagcagatt atgcgacgg accgcaaag atgccccta 
        gctattgaa gccgctttc tatcccgac ttgctcgaa 
        gagctccaa aaaaacaac gcacttttg gaacaaatc 
        atgaagtgt ctcgaatcc tacttggaa gtgaaacgt 
        gtggcattc cctcgtttc tacttccta tcaaacgac 
        gaacttttg gagattttg gcccaaacc cgaaaccca 
        cacgccgtc caaccccac ttgcgtaaa tgtttcgac 
        gcaattgct cgtctagaa tttgcagca aaagaggaa 
        gaagtcggc ataacacct ggcggtgac gaagatgtg 
        aaaaaatcc aaatctgtg acgatgctc acgacaaat 
        atttcggca atgatcagc cctgaaggc gaacgcgtg 
        gcactgact aagggcttg aaagcgcga ggaaacgtc 
        gaggattgg ctgggaaaa gtggaatcg tctatgttt 
        ttcgctctg agacgtttg atgaaaggg gcactgatc 
        gatttccag caaagtcgg agaattgaa tgggtggtg 
        agacacccg agtcagatc accttgact gtgtcgcaa 
        atcatgtgg gccagaggt gtccacgcg attttggac 
        cacggaaac gcccacagt aatttggaa aagtttgag 
        cagaaatgt atcggagat ttgaacgat ttggccact 
        ttgattcgc tcggatttg gattcagtg actcgaaaa 
        gtcttgatt gcgttaatt acgatcgat gtccatgcc 
        agagatacc atcaggaac atggtcgag cacggagtc 
        aaaaaaagc gatagtttc gactggttg aaagtcctg 
        aggtactac tgggacgaa catcttgac gattgcgtt 
        acaagaatg tcttcggct tactacatt tacggttac 
        gagtatttg ggggcgtct ggtgtcctg gttataacc 
        cccttaacc gaccgctgc tacctctgt ttgatgggt 
        gccctccag ctggactta gggggcgct cctgcaggt 
        ccagcggga actggcaaa accgaaacc actaaagat 
        ttggccaaa gctttggcc attcagtgc gtggtgttt 
        aactgttcg gaaggtttg gattataag attatgggt 
        cggtttttt gccggattg gcacagtcc ggtgcttgg 
        agttgtttc gatgagttt aatcgaatc gatattgag 
        gtgctttcg gtgattgcg caacaaatt atcaccata 
        cggaacgcc aaagctgcg aaattgaca cggttcatg 
        tttgaggga cgcgaaatt aagctggtg cagaagtgt 
        gccactttt attacgatg aatccggga tatgccgga 
        aggactgaa cttccggat aatctgaag gctttgttt 
        aggcctatt gcgatgatg gtccccgat tacgctctc 
        atcgccgaa gtgattttg tattcggaa ggtttcgaa 
        tcgtcaaaa gttctctct caaaaaatg gtgcaaatg 
        tataagctt tgtagtgag caattgtcc caacaggat 
        cactatgat tttgggatg cgtgccgtt aagagtgtc 
        ttggtgatg gcaggctcc ctgaagagg gcaaacccc 
        gaccgaaac gaggacgtg gtgttaatt tgtgcgttg 
        cgcgacagt aatctaccg aaatttttg gccgacgat 
        gctctcctt ttccaagga attttgagc gacttgttt 
        cccggagtt gagttaccg gaacaggat tacggtaag 
        ttccaggag gccatcgtg gacgtcatg gtgcaccaa 
        cagctccaa ccggaaaaa cccatgatt cgaaaagtg 
        attcaattg tacgaaacc atgattgtt cgatggggc 
        gtgatgttg gtggggccc acagggggt gggaaaagc 
        acagtttta aacactttg aataaagcc ttgacgaaa 
        atgtacaat gatggtatc gaagggcct tattatcac 
        ccagttcac acttatacc atgaaccca aaggctgta 
        accgctggt gagttgtac ggagaggta aacatttat 
        acgctggaa tggcgcgat ggtctcatg ggtatcatg 
        atgcgaact gctgtgcaa tgtacggaa gaagaccac 
        caatggatt atttgtgac ggtccagtg gatgctgtt 
        tggattgaa aacttaaac accgttttg gacgacaat 
        aaaatgttg tgtcttgca aattcggag cgtattaaa 
        ctcacacct tacgtccat atggtgttc gaagtgatg 
        gatctggca caggcttct ccagctact gtcagtcgt 
        tgtggtatg gtctatata gaccctgat gaaattggc 
        tggttgcct tatgccata tcgtgggtg caaagacgc 
        gacgaagag cttcttaac cacgaattg aaacagttt 
        atgattggt ttgtttgaa tatgctgtt gaaaatggt 
        tttgctttt gtgaaaaaa aatggagat tactctata 
        caccaagtg gatataagt aaagttgca atgctttgt 
        gccatcatt gagagttat ttgaattca ccgaatgcg 
        ttggaaaat atcggcgaa aagagcaaa gttaagagc 
        tacttgtgt caagtgttt attttcgcc tatatttgg 
        tcacttggc gggaatctt accgacgcg tcaaaggaa 
        aagtttgaa gtttatgtg agggaacag tttgatgac 
        catccagat gcaaggttg cctccagga gtcgacctc 
        tacggcgta tttatgaac acgcaagac caccgcttg 
        gacccatgg gcaaaaatc ctcccaacc ttcacctac 
        aaacaagaa gtaccattt tttgaaatg ttggtacca 
        accaacgac actgtccga tttggttac gtcatggaa 
        cgtctaatg tacgtaaat tatcctgta ctattcgtc 
        ggagatact ggtgttggc aaaacagtc gttgcaaaa 
        gacgtcctt aaccgttta tatgaaacg ggtcaattt 
        gttccggtg actataaat ttttcggct caaactagc 
        agttttcga acacaggaa attctcgaa ttgaagctt 
        gaaaaaaag aaaaaaact cttcttggt gccccattg 
        ggcaaaaaa gtgttactt ttcgtcgat gatgtcaat 
        atgcccaaa ttggagaca tacggggcc caacccccg 
        attgaatta ctacgacag tttctaacc tatggtggt 
        ttgtacgat cgggagaaa ttattttgg aaggaaatt 
        cgcgatgtg attgtgtcg gcggcttgt gcaccccct 
        gggggcggc aggaacccg ctcacgccc cgatttgtg 
        cgatttttc gcaatgtta ctaatccca ccaccgaat 
        gagttctcc ctcaaggcg atttttaag gcgatttta 
        aaaggtttt ttctttgat ttttcgaac gaaatcaga 
        gatttggcc gactatatg gtgggggct gccgttgaa 
        atttacatg cggattgcg accgatttg ttgccaact 
        cctgcaaaa tctcactac gtgttcaat ctacgggat 
        ttatcgaaa tgtgtgcaa ggggtgctc caggcggat 
        tctggcacc atgagggag gaaagtgca atgttgcgt 
        ctgttctac cacgagtgt ttgcgcgtt tttcacgat 
        cgattgatc aatgttgag gataaatcg tacttttac 
        tttctgatg agggagatt tgtggtaga aatttcggt 
        actgcggtt ctagccttg ccagatcag ccggttata 
        accaatccg cctttgcta cttttcggc gactttatg 
        caatacggc gccaataaa gaggaccgg atttatgag 
        gaaattagg aacgttgac aagattcga agcgtttta 
        caagattat ttggacgat tataatctt ctcgaatcg 
        aaagacatg cggttgata tttttcatg gacgcgatt 
        gaacattgt gttcgtatc gctcgaata ttgcgatct 
        gagagggga aatgccctc cttgtgggg gttggagga 
        atggggaag caaagtttg acgcgatta gcttcacac 
        gtgaatgct tacaagtgt tttcaaatt gaactgacg 
        cgaaactat gaccggtcg tactttttt gaggatttg 
        agaaaaatg tattttaat gctggagcg aataatgca 
        aattcggtt ttcctgttt accgatacg caaatcgtg 
        caagaagat tttttggaa gatattaac aatattcta 
        aattcgggc gaagtgccg aatcttttt gaggccgac 
        gaatacgaa aaagtgata atagctacc agagatccg 
        gcaaaaggt gcaggagtc gatcctgca aaccgtgac 
        ggtatttac gactatttt ataagcaga gttcgcaat 
        aatttgcat ttggtgatc tgtatgagt ccagtgggt 
        gacgctttt aggcgccgt tgtcgcatg tttccctct 
        ttggtcaat tgttgtaca attgattgg ttcgaaaaa 
        tggccacat gaagcccta ttatctgtg tctcaaaat 
        gctttaaaa gacctggga agtgaggaa ctttgccat 
        aacttatcg acgatttgt gtgacgata cacgaaagt 
        gtggaagaa atgactgaa cggttttat ctcgaaatg 
        agacgacat tattacacg acaccgagt agctacctc 
        gaattgttg aaactgtac cgaaaatta ctcgaaacg 
        aaaaaggaa caagttatt tacaaacga tccagaatt 
        agtaacgga ttacaaaaa ttgtacgaa acgaattca 
        gttatcgaa acaatgaaa gaaactctg attgagctg 
        gaaccagtt ttggccgaa aaatcggtc gctgtggac 
        gaactaatg tcagatttg actacggaa caacaccaa 
        gccgacaaa gtccgagct attgtcaaa tacgacgag 
        gaaattgcc aaagcaaaa gccgaagat actcaagca 
        ttggctgac gatgctcaa agagacctg gatacggca 
        atgccggct ttggaagct gcaaccaaa gctcttgaa 
        gcactcaac aaaaacgat atcaacgaa attaaagtg 
        tttcaaaag ccgccgaaa cttgtacag tatgttatg 
        gaatcggtt tgtctgcta ttaggtgcc aaaactgac 
        tgggcctca gctaagatt gttctcggc gatgttaat 
        ttcttaaag aagcttcag gagtacgac aagaatcac 
        ataactgaa caaactcta cgtaagttg aagtcgtac 
        gttgataat ccagatttt gttcctgat aaagtcggg 
        agagtgtca aaagcgtgc aaaagcatg tgcatgtgg 
        gtgcgtgcg atggatatg tatgcaaaa gtttacaaa 
        attgtggag ccgaagaga aagagattg gaacaagcc 
        gaaaaagag ctcaaccaa gtgatgggc cttttgcgc 
        gagaaacag cgccaactc gccgaagtc gaagcaatg 
        atcgcttca ctcgaagcc aaattcaac caaactctg 
        gcggaaaaa gacgccctc gagaacgaa atggaatta 
        acctccaac agattgaac cgtgccggc cgtttaaac 
        gttgctctc ggcgacgaa caagctcga tgggagcgt 
        tcagtcaaa gagttcgcc gtagaactc caaaacatc 
        atcggcgat gctttaatt gccgctgct tgtgtggcc 
        tatttgggc gcttttacc agcttgtat cgcaacgaa 
        ttggtcgat ctatgggtc tcgcaattt aaagagttc 
        caaataccg gcttcggat aatttcagt ttgattcga 
        gttttggcc gatccgtac gatattcgc atgtggaac 
        tctttcgga ctaccccgt gacactgtt tcgactgaa 
        aatgcaatt ttggtcact caggctagt cgctgggcc 
        cttatgatt gatccgcag gaacaagcc aatcgttgg 
        attcgtcaa atggaagct gcgaacgat ttgcgagtg 
        gtgaaactg accgattcg aattttttg cgcgttttg 
        gagagcgcg attcgaatt ggtaaaccg gtgcttttg 
        gaggaggtg ggcgagacg ttggatccg actttgggc 
        ccgattttg accaaacag acgtttatg caggctgga 
        cggcttttg atacgtttg ggggattcg gatgttgag 
        tatgatccg aattttcga ttttatgtt acaacgaaa 
        ttggcgaat ccgcattat ttgcccgaa atttgtatt 
        caagtgacg attgttaat tttacggtg accaagtcg 
        gggttggag gatcagttg ttagctgat gttgtgcgt 
        ttagaacga cccgattta gagtcacaa agaacagaa 
        ctgattata cgaatcaat aacgacaaa acacagttg 
        caattaatc gaagataaa attttgaaa ttgttgtat 
        cagtccgaa ggtaatatt ttggacgat gaagaatta 
        attgagact ctaaatgag agcaaagaa acttcagct 
        gtgattgcg gctcgtctt ttagagacc gaatcaacg 
        gaacgtaaa atttcagaa gcgcgcgaa aaataccga 
        actgtgtcc atcaggggc tcagttctt tatttcgtc 
        attgcacaa ctggctgaa attgaccca atgtaccaa 
        tactcactg aaatatttc aaccaaatc ttcaacaca 
        gtgattgaa accagcgaa aaaagcaac gatcttaaa 
        gaacggctc aagattttg ttgcgcgaa atcaccgca 
        tttgtctac acaaacgtt tcaaggggt ttatttgag 
        cgacacaaa ctggttttc agttttatg ctttgcgtt 
        gcgattcac caagaaaag ggagaaatt agcgacaac 
        caatggaat tttttgctc agaggtcca gtcggggcc 
        aaaattgaa ttgccccca aaaccggat tatccccta 
        attacggac gctatgtgg ctttcggcc aatttcttg 
        gccgtttcg attccaggc tttgagaaa ttgccagaa 
        gagatcaca aatgtgata agtgtcaga atttctgat 
        ttccagcag gatatttcg gtggtgcca aatgccaag 
        tcgagtcga gtcaaatgg aacgagctt ttggacgat 
        tttaacaag ttgttgctt ttgaaaacc ttgaaagag 
        gagaaattg gtgtttgcc atcaccgaa tatgttaaa 
        atcaaactg gggaaacaa tttatcgaa agtccgcaa 
        gtctcgctt caagttttg tatcaggac acgtcaaat 
        ataactcct ttggtgttt atcctgagc acgggttca 
        gatccgttt gggtcgttc caacgtttt gccgatgaa 
        atgggtttt agggaaagg atcaagtcg atttcgctt 
        ggacaagga caaggtcct gttgctgaa aagataatc 
        gaacaagga ttggaacgt ggcgattgg gtctttctc 
        caaaattgt catttggca acgtcttgg atgttggca 
        atggaacgc ctggttata aaaattgcc gaacagtcg 
        tccaaagtc cacaaggaa ttccgcctc tacatgagt 
        tcaatgcca tcgaagtcg tttcccgtt tcagttttg 
        caaaattcg gtcaaagtg accaacgag ccaccgaaa 
        gggattagg gccaatatt aaaagggcc ttcaccgat 
        atgcaacat gactttttc gaagatcat cctttaaag 
        caagactgg agatgcatg attttcggt acttgcatg 
        tttcatgca ataatacag gaacgaaaa aagtttggc 
        cctttgggg tggaacatc acttatgag tttaacgac 
        agcgatcgc gaatttgcg ttcaatact ttgaaaatg 
        ttttgtgct gaagggacg atcccttgg gacgcttta 
        gagtattta acaggggag attacctac ggtggacgt 
        gttactgat tattgggac ttgaggtgt ttgaaaacg 
        attttgaaa atcttcttc tcgcctcaa atacttaaa 
        ccgcactat aagtactct cctagtggg atttattac 
        tgccctagc tactctaaa cttgagaaa tatcaagag 
        tttatcgac gggcttccg attttggaa gcaccggag 
        attttcgga atgcatgaa aatgcgaac attgcctat 
        caaatcaaa gagactcag aacatactt ttgacgatt 
        atggaaagt caaccgcac acttctggc ggtgctgaa 
        gggcaacaa accgataac attgtctac gatttggcg 
        aatttggtc actgattct ttgatgacg aaaatctcg 
        actgacgag gccaacgtc aacatgttt aaaagagac 
        gacaagggc cgtttgccc tcactcaca acagtccta 
        atgcaagaa gtcgaccgt tacaacatt ttactcaaa 
        ctgatacac agttcgatg gataatttg aagaaagca 
        atcaaaggt ttggtcgta atgtcggac gctttggag 
        gaggtctac gttgcattt accaacaat caggtgcca 
        aaaatgtgg aacaaaaaa gcctacaat tcgttaaaa 
        agtctcgga agttggata cgagacttg gtgttgcgg 
        ctcgatttt attaaaatt tgggtacga tcgggtcca 
        ccgtcttcc tattggtta tcagggttt tatttccca 
        caaggtttc ttaacgggg actttacaa acccatgcc 
        agaaagtat aatttaccg atcgatcaa ctcaaattt 
        gattttgac gtacaaaaa gtgtggatt gagcaggag 
        caagtgaag aaaattcat gatgaggaa caacatgaa 
        aatttggaa gtttataaa ggcttgcat catccggaa 
        gatggggct ataattcat ggtctgttc ctggatgca 
        ggtcgctgg gattccccg actcataaa ttggttgat 
        gctaaacca ggtgaaatt aatccattg ttgccggcc 
        atttggatg ttaccaaag acttcgtta cctcctaat 
        gataagaga tatgtcact ccgttatac aaaacttca 
        atacgtgca ggtgtcttg tccacaaca gggcacagc 
        acaaatttt gtgattgct gtcttgtta ccaaccgat 
        aaaccacaa agttattgg attcttaaa ggaactgca 
        ttattaaca caagtcact gattgatta tcttttctt 
        agttgtttt tgcaatgta ttgttgctt aagaataaa 
        gtgtttttt tttaa
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