| General information | |||
| TC000119 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 10911915..10950309 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC000119 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02979 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC000119 RA
MDEPFGEIP KSRLSSKTE HRFDELERV DEKGKVENK LICKPVQSF HRDSNVISF HIPHKKLLE KIEYKKSPP EPLAGFSNI QQDLLSRKR SASEDLNIS SSDDEESII SDVSRFALK YRRSTLSPS VAKEPVLPH AKLYDVMDQ NEWIAKLRQ AADGYFIYM TYTVPQSSE LYSPYALSV VPFPKVDKS NFFTMSSKG VMQHQGVET VFTPLDVWE REFQMYCKL MHFRTFYRF RLWKGFYVW RKAVVRKKF KSAQVYLNE NLFILNNEL RAGLLAIQQ MCYKMTDIS FIDDDTIED FLLFYFIEN QMEKLESVS DKLQEFREV AKDIVANAC HGALLAKGF VPNELLVEP SKDRKRGGV VTKAQAKKT SYIEQAAKK KFCMRLTCF IILADYLMI DLMHNLITR STRDLNAIL EKHVQFLPS KEKIEAATI TEILEEPRP EEAPQLPLF IIDVVLNAK KLSLDPSLR VTLNIFNQV MDLWQEHCK KIKSFTADE MYRIFTNPI INGKEEARI CGYGPTLYF YLDDDKKLK DSIQQIVEH FVHNYKMAK LYVSKYNFI REFFAEDEQ VKEEALKEE RDIETFRNM CNRYHKELD IIDGVIVSQ SLGMLNLQL SHLKECAKP EPERLISAL THILPWIGK NEIDRLIEE SENMETSLN QQPVTTVDY VKYLEYLDD ANTKLNDME NNLDYCKEL YDIMEEFAI PVPDEDMNN YLGVSVTMA SLRNLVDRK LDEINKTIK SFNDQMNKD ISALISEVG AIKDECMQP WLYDIESNI GEVTEFLND LYERLVTCQ QRAAEFKGY QKQFRLEVT RFDILDEVM ADVKLRMLL WESVTSWSK TVDEWYHSD FSELNVEDM NLYTAKTIK NINLLEKGL PKNLIVPKL KDDVELMKT KLPVVAYLR NPSLRQRHW IQVENILSY KFKPDDEVT LELLENLKV FSFANELQE ISGQASSEA SLEQLLKKV EEAWKTLEF QVMLHKDSK DVYILGSLE EVQSVLDDS TINITTIAS SRHVGPIKS RVEEWARQL DLFSRTLDE WVACQQSWL YLEVIFSAP DIQRQLPSE SKLFIIVDK SWKQIMRRT AKMPLAIEA AFYPDLLEE LQKNNALLE QIMKCLESY LEVKRVAFP RFYFLSNDE LLEILAQTR NPHAVQPHL RKCFDAIAR LEFAAKEEE VGITPGGDE DVKKSKSVT MLTTNISAM ISPEGERVA LTKGLKARG NVEDWLGKV ESSMFFALR RLMKGALID FQQSRRIEW VVRHPSQIT LTVSQIMWA RGVHAILDH GNAHSNLEK FEQKCIGDL NDLATLIRS DLDSVTRKV LIALITIDV HARDTIRNM VEHGVKKSD SFDWLKVLR YYWDEHLDD CVTRMSSAY YIYGYEYLG ASGVLVITP LTDRCYLCL MGALQLDLG GAPAGPAGT GKTETTKDL AKALAIQCV VFNCSEGLD YKIMGRFFA GLAQSGAWS CFDEFNRID IEVLSVIAQ QIITIRNAK AAKLTRFMF EGREIKLVQ KCATFITMN PGYAGRTEL PDNLKALFR PIAMMVPDY ALIAEVILY SEGFESSKV LSQKMVQMY KLCSEQLSQ QDHYDFGMR AVKSVLVMA GSLKRANPD RNEDVVLIC ALRDSNLPK FLADDALLF QGILSDLFP GVELPEQDY GKFQEAIVD VMVHQQLQP EKPMIRKVI QLYETMIVR WGVMLVGPT GGGKSTVLN TLNKALTKM YNDGIEGPY YHPVHTYTM NPKAVTAGE LYGEVNIYT LEWRDGLMG IMMRTAVQC TEEDHQWII CDGPVDAVW IENLNTVLD DNKMLCLAN SERIKLTPY VHMVFEVMD LAQASPATV SRCGMVYID PDEIGWLPY AISWVQRRD EELLNHELK QFMIGLFEY AVENGFAFV KKNGDYSIH QVDISKVAM LCAIIESYL NSPNALENI GEKSKVKSY LCQVFIFAY IWSLGGNLT DASKEKFEV YVREQFDDH PDARLPPGV DLYGVFMNT QDHRLDPWA KILPTFTYK QEVPFFEML VPTNDTVRF GYVMERLMY VNYPVLFVG DTGVGKTVV AKDVLNRLY ETGQFVPVT INFSAQTSS FRTQEILEL KLEKKKKTL LGAPLGKKV LLFVDDVNM PKLETYGAQ PPIELLRQF LTYGGLYDR EKLFWKEIR DVIVSAACA PPGGGRNPL TPRFVRFFA MLLIPPPNE FSLKAIFKA ILKGFFFDF SNEIRDLAD YMVGAAVEI YMRIATDLL PTPAKSHYV FNLRDLSKC VQGVLQADS GTMREESAM LRLFYHECL RVFHDRLIN VEDKSYFYF LMREICGRN FGTAVLALP DQPVITNPP LLLFGDFMQ YGANKEDRI YEEIRNVDK IRSVLQDYL DDYNLLESK DMRLIFFMD AIEHCVRIA RILRSERGN ALLVGVGGM GKQSLTRLA SHVNAYKCF QIELTRNYD RSYFFEDLR KMYFNAGAN NANSVFLFT DTQIVQEDF LEDINNILN SGEVPNLFE ADEYEKVII ATRDPAKGA GVDPANRDG IYDYFISRV RNNLHLVIC MSPVGDAFR RRCRMFPSL VNCCTIDWF EKWPHEALL SVSQNALKD LGSEELCHN LSTICVTIH ESVEEMTER FYLEMRRHY YTTPSSYLE LLKLYRKLL ETKKEQVIY KRSRISNGL QKLYETNSV IETMKETLI ELEPVLAEK SVAVDELMS DLTTEQHQA DKVRAIVKY DEEIAKAKA EDTQALADD AQRDLDTAM PALEAATKA LEALNKNDI NEIKVFQKP PKLVQYVME SVCLLLGAK TDWASAKIV LGDVNFLKK LQEYDKNHI TEQTLRKLK SYVDNPDFV PDKVGRVSK ACKSMCMWV RAMDMYAKV YKIVEPKRK RLEQAEKEL NQVMGLLRE KQRQLAEVE AMIASLEAK FNQTLAEKD ALENEMELT SNRLNRAGR LNVALGDEQ ARWERSVKE FAVELQNII GDALIAAAC VAYLGAFTS LYRNELVDL WVSQFKEFQ IPASDNFSL IRVLADPYD IRMWNSFGL PRDTVSTEN AILVTQASR WALMIDPQE QANRWIRQM EAANDLRVV KLTDSNFLR VLESAIRIG KPVLLEEVG ETLDPTLGP ILTKQTFMQ AGRLLIRLG DSDVEYDPN FRFYVTTKL ANPHYLPEI CIQVTIVNF TVTKSGLED QLLADVVRL ERPDLESQR TELIIRINN DKTQLQLIE DKILKLLYQ SEGNILDDE ELIETLNES KETSAVIAA RLLETESTE RKISEAREK YRTVSIRGS VLYFVIAQL AEIDPMYQY SLKYFNQIF NTVIETSEK SNDLKERLK ILLREITAF VYTNVSRGL FERHKLVFS FMLCVAIHQ EKGEISDNQ WNFLLRGPV GAKIELPPK PDYPLITDA MWLSANFLA VSIPGFEKL PEEITNVIS VRISDFQQD ISVVPNAKS SRVKWNELL DDFNKLLLL KTLKEEKLV FAITEYVKI KLGKQFIES PQVSLQVLY QDTSNITPL VFILSTGSD PFGSFQRFA DEMGFRERI KSISLGQGQ GPVAEKIIE QGLERGDWV FLQNCHLAT SWMLAMERL VIKIAEQSS KVHKEFRLY MSSMPSKSF PVSVLQNSV KVTNEPPKG IRANIKRAF TDMQHDFFE DHPLKQDWR CMIFGTCMF HAIIQERKK FGPLGWNIT YEFNDSDRE FAFNTLKMF CAEGTIPWD ALEYLTGEI TYGGRVTDY WDLRCLKTI LKIFFSPQI LKPHYKYSP SGIYYCPSY SKLEKYQEF IDGLPILEA PEIFGMHEN ANIAYQIKE TQNILLTIM ESQPHTSGG AEGQQTDNI VYDLANLVT DSLMTKIST DEANVNMFK RDDKGRLPS LTTVLMQEV DRYNILLKL IHSSMDNLK KAIKGLVVM SDALEEVYV AFTNNQVPK MWNKKAYNS LKSLGSWIR DLVLRLDFI KIWVRSGPP SSYWLSGFY FPQGFLTGT LQTHARKYN LPIDQLKFD FDVQKVWIE QEQVKKIHD EEQHENLEV YKGLHHPED GAIIHGLFL DAGRWDSPT HKLVDAKPG EINPLLPAI WMLPKTSLP PNDKRYVTP LYKTSIRAG VLSTTGHST NFVIAVLLP TDKPQSYWI LKGTALLTQ VTD
- >TC000119 RA
atggatgag ccatttggt gaaatcccg aaatccagg ctgagttcg aaaactgaa cacaggttt gatgagctc gagcgggtc gacgagaag ggcaaagtc gaaaataag ctcatttgc aagcccgtt cagtcgttc cacagagat tccaatgtc atttcattt cacattcca cacaagaaa cttctcgaa aaaatcgaa tataagaaa tcgccaccc gaacctttg gccggtttc tcaaacatc caacaagac ttattgagt cgcaaacga agcgcttct gaagacctt aatatcagt tcttcggac gacgaagaa tcaataatc agtgatgtt tcacgattt gctttaaaa taccgacgg agtactctt tcgccgtct gtggcaaaa gaacccgtc cttcctcac gccaaactt tacgatgtc atggatcaa aacgaatgg atcgcaaaa ttacgacaa gccgccgat ggttatttc atttatatg acttacacg gtaccacaa tcttcagaa ctttactcc ccttacgcc cttagcgtt gtgcctttt ccaaaagta gacaagagt aattttttc acaatgagt tccaaaggt gtgatgcaa caccaaggg gttgaaacg gtgttcacc cctctggac gtgtgggaa cgcgagttt caaatgtac tgcaaactg atgcacttc cgcacattt tatcgtttc cgattgtgg aaaggtttc tacgtttgg cggaaagct gtagttcgt aaaaaattc aaatcggca caagtttac ctcaacgaa aacttgttt attttaaat aatgaactg cgagcgggt ctcttggcc atccaacaa atgtgctac aaaatgacg gatatttcc ttcattgac gacgacacc atcgaggat tttctccta ttttatttc attgaaaat cagatggaa aaactcgaa tcagtgtcg gacaaactg caagaattt cgcgaagtg gccaaagat attgtcgcg aatgcgtgc catggggct cttttagcc aaaggtttc gtcccaaat gagttactc gttgagcca agcaaagac cgtaaacgt ggtggtgtt gtgaccaaa gctcaggcc aagaaaacc tcgtacatt gagcaagcg gccaagaaa aaattctgt atgcgtctc acgtgtttt attattctt gccgattat ttaatgatt gatttgatg cacaatttg attacgcgg tcgacgcgc gatcttaat gccattttg gaaaaacac gttcagttt ttgccctcg aaggaaaaa atcgaggct gcgaccata acggagatt ttggaggag ccaagacca gaggaggct cctcagctt cctttattt atcattgat gttgttttg aatgcgaaa aaattgtcg ctggatccg tctctcaga gtcactttg aacattttc aatcaagtg atggatttg tggcaggag cattgtaaa aaaattaag tcgtttacg gccgatgaa atgtatcgg attttcaca aatccgata ataaacggt aaagaggag gctcgtatt tgtggctat ggccccact ttatatttc tatttagac gacgataaa aaattgaaa gattccata caacaaatc gttgaacat ttcgtccat aattataaa atggccaag ctctacgtt agtaaatat aatttcatt cgggaattt ttcgccgaa gacgagcaa gtcaaggaa gaagccttg aaggaggaa agagatatt gaaacgttt cgaaatatg tgtaaccgc taccataaa gaactcgac attattgac ggtgttatc gtatcgcaa tctcttgga atgttaaat ttgcaactg agccatttg aaagaatgt gcaaaaccg gaaccggaa cgcctcatt tcggccctt acacacata cttccttgg attggtaaa aacgaaatt gatcgtttg attgaagaa tcggagaat atggagacg agtctcaac caacaaccc gtcacaaca gttgactat gtcaagtat ttggaatat ttggacgat gctaatacc aagctgaac gatatggag aataatctg gactattgt aaagaattg tatgacatt atggaggaa tttgcgatc ccggtgccc gacgaagac atgaacaat tatctaggt gttagcgta acgatggct tctttgcgc aatttggtc gaccgaaag ttggacgag attaataaa accatcaag agttttaac gatcagatg aacaaggat atcagtgct ttgatttcg gaagtggga gccataaaa gacgaatgc atgcaacct tggttgtac gatattgag tcaaatatc ggcgaagtg accgaattt ttgaacgat ttgtacgaa cgacttgtc acttgtcag cagagagcg gctgaattt aaaggctac cagaaacaa tttcggttg gaagtgaca cgttttgat attttggac gaagttatg gcagacgtg aagttacgt atgctcctg tgggagagt gtcacctcc tggagcaaa accgtggac gagtggtac cactctgat ttcagcgaa ttgaacgtc gaagacatg aacttgtac acggcgaaa actatcaaa aatatcaat cttctggag aagggattg cccaaaaat ttgatcgtt ccgaaacta aaggacgat gtcgagttg atgaagacc aagttgcca gtcgttgca tatttgcgg aacccaagt ctacgacaa cgccactgg atccaagtg gagaacatc ctcagctac aaattcaag ccggacgat gaagtcaca cttgagctt ttggaaaat ttgaaagtt ttcagtttt gcgaatgaa cttcaggag atttcgggt caggcgagt agtgaggcg agtctcgag cagcttttg aagaaggtg gaagaagcg tggaagacg ctcgagttt caagttatg ttgcacaag gattcgaag gatgtttat attttgggt tcgctggag gaggttcag agtgttttg gatgatagt accataaat ataacgaca attgcgagt tcgagacat gtgggtccg attaaatca cgtgtggag gagtgggcc cgacagttg gatttgttt tcgaggacg ctggatgaa tgggtggct tgtcaacag agctggctt tatttggag gtgattttt tccgcgcct gatattcaa agacagctt ccgagcgag tcgaagctt tttatcatt gtggacaag tcgtggaag cagattatg cgacggacc gcaaagatg cccctagct attgaagcc gctttctat cccgacttg ctcgaagag ctccaaaaa aacaacgca cttttggaa caaatcatg aagtgtctc gaatcctac ttggaagtg aaacgtgtg gcattccct cgtttctac ttcctatca aacgacgaa cttttggag attttggcc caaacccga aacccacac gccgtccaa ccccacttg cgtaaatgt ttcgacgca attgctcgt ctagaattt gcagcaaaa gaggaagaa gtcggcata acacctggc ggtgacgaa gatgtgaaa aaatccaaa tctgtgacg atgctcacg acaaatatt tcggcaatg atcagccct gaaggcgaa cgcgtggca ctgactaag ggcttgaaa gcgcgagga aacgtcgag gattggctg ggaaaagtg gaatcgtct atgtttttc gctctgaga cgtttgatg aaaggggca ctgatcgat ttccagcaa agtcggaga attgaatgg gtggtgaga cacccgagt cagatcacc ttgactgtg tcgcaaatc atgtgggcc agaggtgtc cacgcgatt ttggaccac ggaaacgcc cacagtaat ttggaaaag tttgagcag aaatgtatc ggagatttg aacgatttg gccactttg attcgctcg gatttggat tcagtgact cgaaaagtc ttgattgcg ttaattacg atcgatgtc catgccaga gataccatc aggaacatg gtcgagcac ggagtcaaa aaaagcgat agtttcgac tggttgaaa gtcctgagg tactactgg gacgaacat cttgacgat tgcgttaca agaatgtct tcggcttac tacatttac ggttacgag tatttgggg gcgtctggt gtcctggtt ataaccccc ttaaccgac cgctgctac ctctgtttg atgggtgcc ctccagctg gacttaggg ggcgctcct gcaggtcca gcgggaact ggcaaaacc gaaaccact aaagatttg gccaaagct ttggccatt cagtgcgtg gtgtttaac tgttcggaa ggtttggat tataagatt atgggtcgg ttttttgcc ggattggca cagtccggt gcttggagt tgtttcgat gagtttaat cgaatcgat attgaggtg ctttcggtg attgcgcaa caaattatc accatacgg aacgccaaa gctgcgaaa ttgacacgg ttcatgttt gagggacgc gaaattaag ctggtgcag aagtgtgcc acttttatt acgatgaat ccgggatat gccggaagg actgaactt ccggataat ctgaaggct ttgtttagg cctattgcg atgatggtc cccgattac gctctcatc gccgaagtg attttgtat tcggaaggt ttcgaatcg tcaaaagtt ctctctcaa aaaatggtg caaatgtat aagctttgt agtgagcaa ttgtcccaa caggatcac tatgatttt gggatgcgt gccgttaag agtgtcttg gtgatggca ggctccctg aagagggca aaccccgac cgaaacgag gacgtggtg ttaatttgt gcgttgcgc gacagtaat ctaccgaaa tttttggcc gacgatgct ctccttttc caaggaatt ttgagcgac ttgtttccc ggagttgag ttaccggaa caggattac ggtaagttc caggaggcc atcgtggac gtcatggtg caccaacag ctccaaccg gaaaaaccc atgattcga aaagtgatt caattgtac gaaaccatg attgttcga tggggcgtg atgttggtg gggcccaca gggggtggg aaaagcaca gttttaaac actttgaat aaagccttg acgaaaatg tacaatgat ggtatcgaa gggccttat tatcaccca gttcacact tataccatg aacccaaag gctgtaacc gctggtgag ttgtacgga gaggtaaac atttatacg ctggaatgg cgcgatggt ctcatgggt atcatgatg cgaactgct gtgcaatgt acggaagaa gaccaccaa tggattatt tgtgacggt ccagtggat gctgtttgg attgaaaac ttaaacacc gttttggac gacaataaa atgttgtgt cttgcaaat tcggagcgt attaaactc acaccttac gtccatatg gtgttcgaa gtgatggat ctggcacag gcttctcca gctactgtc agtcgttgt ggtatggtc tatatagac cctgatgaa attggctgg ttgccttat gccatatcg tgggtgcaa agacgcgac gaagagctt cttaaccac gaattgaaa cagtttatg attggtttg tttgaatat gctgttgaa aatggtttt gcttttgtg aaaaaaaat ggagattac tctatacac caagtggat ataagtaaa gttgcaatg ctttgtgcc atcattgag agttatttg aattcaccg aatgcgttg gaaaatatc ggcgaaaag agcaaagtt aagagctac ttgtgtcaa gtgtttatt ttcgcctat atttggtca cttggcggg aatcttacc gacgcgtca aaggaaaag tttgaagtt tatgtgagg gaacagttt gatgaccat ccagatgca aggttgcct ccaggagtc gacctctac ggcgtattt atgaacacg caagaccac cgcttggac ccatgggca aaaatcctc ccaaccttc acctacaaa caagaagta ccatttttt gaaatgttg gtaccaacc aacgacact gtccgattt ggttacgtc atggaacgt ctaatgtac gtaaattat cctgtacta ttcgtcgga gatactggt gttggcaaa acagtcgtt gcaaaagac gtccttaac cgtttatat gaaacgggt caatttgtt ccggtgact ataaatttt tcggctcaa actagcagt tttcgaaca caggaaatt ctcgaattg aagcttgaa aaaaagaaa aaaactctt cttggtgcc ccattgggc aaaaaagtg ttacttttc gtcgatgat gtcaatatg cccaaattg gagacatac ggggcccaa cccccgatt gaattacta cgacagttt ctaacctat ggtggtttg tacgatcgg gagaaatta ttttggaag gaaattcgc gatgtgatt gtgtcggcg gcttgtgca ccccctggg ggcggcagg aacccgctc acgccccga tttgtgcga tttttcgca atgttacta atcccacca ccgaatgag ttctccctc aaggcgatt tttaaggcg attttaaaa ggttttttc tttgatttt tcgaacgaa atcagagat ttggccgac tatatggtg ggggctgcc gttgaaatt tacatgcgg attgcgacc gatttgttg ccaactcct gcaaaatct cactacgtg ttcaatcta cgggattta tcgaaatgt gtgcaaggg gtgctccag gcggattct ggcaccatg agggaggaa agtgcaatg ttgcgtctg ttctaccac gagtgtttg cgcgttttt cacgatcga ttgatcaat gttgaggat aaatcgtac ttttacttt ctgatgagg gagatttgt ggtagaaat ttcggtact gcggttcta gccttgcca gatcagccg gttataacc aatccgcct ttgctactt ttcggcgac tttatgcaa tacggcgcc aataaagag gaccggatt tatgaggaa attaggaac gttgacaag attcgaagc gttttacaa gattatttg gacgattat aatcttctc gaatcgaaa gacatgcgg ttgatattt ttcatggac gcgattgaa cattgtgtt cgtatcgct cgaatattg cgatctgag aggggaaat gccctcctt gtgggggtt ggaggaatg gggaagcaa agtttgacg cgattagct tcacacgtg aatgcttac aagtgtttt caaattgaa ctgacgcga aactatgac cggtcgtac ttttttgag gatttgaga aaaatgtat tttaatgct ggagcgaat aatgcaaat tcggttttc ctgtttacc gatacgcaa atcgtgcaa gaagatttt ttggaagat attaacaat attctaaat tcgggcgaa gtgccgaat ctttttgag gccgacgaa tacgaaaaa gtgataata gctaccaga gatccggca aaaggtgca ggagtcgat cctgcaaac cgtgacggt atttacgac tattttata agcagagtt cgcaataat ttgcatttg gtgatctgt atgagtcca gtgggtgac gcttttagg cgccgttgt cgcatgttt ccctctttg gtcaattgt tgtacaatt gattggttc gaaaaatgg ccacatgaa gccctatta tctgtgtct caaaatgct ttaaaagac ctgggaagt gaggaactt tgccataac ttatcgacg atttgtgtg acgatacac gaaagtgtg gaagaaatg actgaacgg ttttatctc gaaatgaga cgacattat tacacgaca ccgagtagc tacctcgaa ttgttgaaa ctgtaccga aaattactc gaaacgaaa aaggaacaa gttatttac aaacgatcc agaattagt aacggatta caaaaattg tacgaaacg aattcagtt atcgaaaca atgaaagaa actctgatt gagctggaa ccagttttg gccgaaaaa tcggtcgct gtggacgaa ctaatgtca gatttgact acggaacaa caccaagcc gacaaagtc cgagctatt gtcaaatac gacgaggaa attgccaaa gcaaaagcc gaagatact caagcattg gctgacgat gctcaaaga gacctggat acggcaatg ccggctttg gaagctgca accaaagct cttgaagca ctcaacaaa aacgatatc aacgaaatt aaagtgttt caaaagccg ccgaaactt gtacagtat gttatggaa tcggtttgt ctgctatta ggtgccaaa actgactgg gcctcagct aagattgtt ctcggcgat gttaatttc ttaaagaag cttcaggag tacgacaag aatcacata actgaacaa actctacgt aagttgaag tcgtacgtt gataatcca gattttgtt cctgataaa gtcgggaga gtgtcaaaa gcgtgcaaa agcatgtgc atgtgggtg cgtgcgatg gatatgtat gcaaaagtt tacaaaatt gtggagccg aagagaaag agattggaa caagccgaa aaagagctc aaccaagtg atgggcctt ttgcgcgag aaacagcgc caactcgcc gaagtcgaa gcaatgatc gcttcactc gaagccaaa ttcaaccaa actctggcg gaaaaagac gccctcgag aacgaaatg gaattaacc tccaacaga ttgaaccgt gccggccgt ttaaacgtt gctctcggc gacgaacaa gctcgatgg gagcgttca gtcaaagag ttcgccgta gaactccaa aacatcatc ggcgatgct ttaattgcc gctgcttgt gtggcctat ttgggcgct tttaccagc ttgtatcgc aacgaattg gtcgatcta tgggtctcg caatttaaa gagttccaa ataccggct tcggataat ttcagtttg attcgagtt ttggccgat ccgtacgat attcgcatg tggaactct ttcggacta ccccgtgac actgtttcg actgaaaat gcaattttg gtcactcag gctagtcgc tgggccctt atgattgat ccgcaggaa caagccaat cgttggatt cgtcaaatg gaagctgcg aacgatttg cgagtggtg aaactgacc gattcgaat tttttgcgc gttttggag agcgcgatt cgaattggt aaaccggtg cttttggag gaggtgggc gagacgttg gatccgact ttgggcccg attttgacc aaacagacg tttatgcag gctggacgg cttttgata cgtttgggg gattcggat gttgagtat gatccgaat tttcgattt tatgttaca acgaaattg gcgaatccg cattatttg cccgaaatt tgtattcaa gtgacgatt gttaatttt acggtgacc aagtcgggg ttggaggat cagttgtta gctgatgtt gtgcgttta gaacgaccc gatttagag tcacaaaga acagaactg attatacga atcaataac gacaaaaca cagttgcaa ttaatcgaa gataaaatt ttgaaattg ttgtatcag tccgaaggt aatattttg gacgatgaa gaattaatt gagactcta aatgagagc aaagaaact tcagctgtg attgcggct cgtctttta gagaccgaa tcaacggaa cgtaaaatt tcagaagcg cgcgaaaaa taccgaact gtgtccatc aggggctca gttctttat ttcgtcatt gcacaactg gctgaaatt gacccaatg taccaatac tcactgaaa tatttcaac caaatcttc aacacagtg attgaaacc agcgaaaaa agcaacgat cttaaagaa cggctcaag attttgttg cgcgaaatc accgcattt gtctacaca aacgtttca aggggttta tttgagcga cacaaactg gttttcagt tttatgctt tgcgttgcg attcaccaa gaaaaggga gaaattagc gacaaccaa tggaatttt ttgctcaga ggtccagtc ggggccaaa attgaattg cccccaaaa ccggattat cccctaatt acggacgct atgtggctt tcggccaat ttcttggcc gtttcgatt ccaggcttt gagaaattg ccagaagag atcacaaat gtgataagt gtcagaatt tctgatttc cagcaggat atttcggtg gtgccaaat gccaagtcg agtcgagtc aaatggaac gagcttttg gacgatttt aacaagttg ttgcttttg aaaaccttg aaagaggag aaattggtg tttgccatc accgaatat gttaaaatc aaactgggg aaacaattt atcgaaagt ccgcaagtc tcgcttcaa gttttgtat caggacacg tcaaatata actcctttg gtgtttatc ctgagcacg ggttcagat ccgtttggg tcgttccaa cgttttgcc gatgaaatg ggttttagg gaaaggatc aagtcgatt tcgcttgga caaggacaa ggtcctgtt gctgaaaag ataatcgaa caaggattg gaacgtggc gattgggtc tttctccaa aattgtcat ttggcaacg tcttggatg ttggcaatg gaacgcctg gttataaaa attgccgaa cagtcgtcc aaagtccac aaggaattc cgcctctac atgagttca atgccatcg aagtcgttt cccgtttca gttttgcaa aattcggtc aaagtgacc aacgagcca ccgaaaggg attagggcc aatattaaa agggccttc accgatatg caacatgac tttttcgaa gatcatcct ttaaagcaa gactggaga tgcatgatt ttcggtact tgcatgttt catgcaata atacaggaa cgaaaaaag tttggccct ttggggtgg aacatcact tatgagttt aacgacagc gatcgcgaa tttgcgttc aatactttg aaaatgttt tgtgctgaa gggacgatc ccttgggac gctttagag tatttaaca ggggagatt acctacggt ggacgtgtt actgattat tgggacttg aggtgtttg aaaacgatt ttgaaaatc ttcttctcg cctcaaata cttaaaccg cactataag tactctcct agtgggatt tattactgc cctagctac tctaaactt gagaaatat caagagttt atcgacggg cttccgatt ttggaagca ccggagatt ttcggaatg catgaaaat gcgaacatt gcctatcaa atcaaagag actcagaac atacttttg acgattatg gaaagtcaa ccgcacact tctggcggt gctgaaggg caacaaacc gataacatt gtctacgat ttggcgaat ttggtcact gattctttg atgacgaaa atctcgact gacgaggcc aacgtcaac atgtttaaa agagacgac aagggccgt ttgccctca ctcacaaca gtcctaatg caagaagtc gaccgttac aacatttta ctcaaactg atacacagt tcgatggat aatttgaag aaagcaatc aaaggtttg gtcgtaatg tcggacgct ttggaggag gtctacgtt gcatttacc aacaatcag gtgccaaaa atgtggaac aaaaaagcc tacaattcg ttaaaaagt ctcggaagt tggatacga gacttggtg ttgcggctc gattttatt aaaatttgg gtacgatcg ggtccaccg tcttcctat tggttatca gggttttat ttcccacaa ggtttctta acggggact ttacaaacc catgccaga aagtataat ttaccgatc gatcaactc aaatttgat tttgacgta caaaaagtg tggattgag caggagcaa gtgaagaaa attcatgat gaggaacaa catgaaaat ttggaagtt tataaaggc ttgcatcat ccggaagat ggggctata attcatggt ctgttcctg gatgcaggt cgctgggat tccccgact cataaattg gttgatgct aaaccaggt gaaattaat ccattgttg ccggccatt tggatgtta ccaaagact tcgttacct cctaatgat aagagatat gtcactccg ttatacaaa acttcaata cgtgcaggt gtcttgtcc acaacaggg cacagcaca aattttgtg attgctgtc ttgttacca accgataaa ccacaaagt tattggatt cttaaagga actgcatta ttaacacaa gtcactgat tga
- >TC000119 RA
tgaaaataa aattcgtgt atctgtgtg aagtgaata caaaactgt taatttttc gaaatggat gagccattt ggtgaaatc ccgaaatcc aggctgagt tcgaaaact gaacacagg tttgatgag ctcgagcgg gtcgacgag aagggcaaa gtcgaaaat aagctcatt tgcaagccc gttcagtcg ttccacaga gattccaat gtcatttca tttcacatt ccacacaag aaacttctc gaaaaaatc gaatataag aaatcgcca cccgaacct ttggccggt ttctcaaac atccaacaa gacttattg agtcgcaaa cgaagcgct tctgaagac cttaatatc agttcttcg gacgacgaa gaatcaata atcagtgat gtttcacga tttgcttta aaataccga cggagtact ctttcgccg tctgtggca aaagaaccc gtccttcct cacgccaaa ctttacgat gtcatggat caaaacgaa tggatcgca aaattacga caagccgcc gatggttat ttcatttat atgacttac acggtacca caatcttca gaactttac tccccttac gcccttagc gttgtgcct tttccaaaa gtagacaag agtaatttt ttcacaatg agttccaaa ggtgtgatg caacaccaa ggggttgaa acggtgttc acccctctg gacgtgtgg gaacgcgag tttcaaatg tactgcaaa ctgatgcac ttccgcaca ttttatcgt ttccgattg tggaaaggt ttctacgtt tggcggaaa gctgtagtt cgtaaaaaa ttcaaatcg gcacaagtt tacctcaac gaaaacttg tttatttta aataatgaa ctgcgagcg ggtctcttg gccatccaa caaatgtgc tacaaaatg acggatatt tccttcatt gacgacgac accatcgag gattttctc ctattttat ttcattgaa aatcagatg gaaaaactc gaatcagtg tcggacaaa ctgcaagaa tttcgcgaa gtggccaaa gatattgtc gcgaatgcg tgccatggg gctctttta gccaaaggt ttcgtccca aatgagtta ctcgttgag ccaagcaaa gaccgtaaa cgtggtggt gttgtgacc aaagctcag gccaagaaa acctcgtac attgagcaa gcggccaag aaaaaattc tgtatgcgt ctcacgtgt tttattatt cttgccgat tatttaatg attgatttg atgcacaat ttgattacg cggtcgacg cgcgatctt aatgccatt ttggaaaaa cacgttcag tttttgccc tcgaaggaa aaaatcgag gctgcgacc ataacggag attttggag gagccaaga ccagaggag gctcctcag cttccttta tttatcatt gatgttgtt ttgaatgcg aaaaaattg tcgctggat ccgtctctc agagtcact ttgaacatt ttcaatcaa gtgatggat ttgtggcag gagcattgt aaaaaaatt aagtcgttt acggccgat gaaatgtat cggattttc acaaatccg ataataaac ggtaaagag gaggctcgt atttgtggc tatggcccc actttatat ttctattta gacgacgat aaaaaattg aaagattcc atacaacaa atcgttgaa catttcgtc cataattat aaaatggcc aagctctac gttagtaaa tataatttc attcgggaa tttttcgcc gaagacgag caagtcaag gaagaagcc ttgaaggag gaaagagat attgaaacg tttcgaaat atgtgtaac cgctaccat aaagaactc gacattatt gacggtgtt atcgtatcg caatctctt ggaatgtta aatttgcaa ctgagccat ttgaaagaa tgtgcaaaa ccggaaccg gaacgcctc atttcggcc cttacacac atacttcct tggattggt aaaaacgaa attgatcgt ttgattgaa gaatcggag aatatggag acgagtctc aaccaacaa cccgtcaca acagttgac tatgtcaag tatttggaa tatttggac gatgctaat accaagctg aacgatatg gagaataat ctggactat tgtaaagaa ttgtatgac attatggag gaatttgcg atcccggtg cccgacgaa gacatgaac aattatcta ggtgttagc gtaacgatg gcttctttg cgcaatttg gtcgaccga aagttggac gagattaat aaaaccatc aagagtttt aacgatcag atgaacaag gatatcagt gctttgatt tcggaagtg ggagccata aaagacgaa tgcatgcaa ccttggttg tacgatatt gagtcaaat atcggcgaa gtgaccgaa tttttgaac gatttgtac gaacgactt gtcacttgt cagcagaga gcggctgaa tttaaaggc taccagaaa caatttcgg ttggaagtg acacgtttt gatattttg gacgaagtt atggcagac gtgaagtta cgtatgctc ctgtgggag agtgtcacc tcctggagc aaaaccgtg gacgagtgg taccactct gatttcagc gaattgaac gtcgaagac atgaacttg tacacggcg aaaactatc aaaaatatc aatcttctg gagaaggga ttgcccaaa aatttgatc gttccgaaa ctaaaggac gatgtcgag ttgatgaag accaagttg ccagtcgtt gcatatttg cggaaccca agtctacga caacgccac tggatccaa gtggagaac atcctcagc tacaaattc aagccggac gatgaagtc acacttgag cttttggaa aatttgaaa gttttcagt tttgcgaat gaacttcag gagatttcg ggtcaggcg agtagtgag gcgagtctc gagcagctt ttgaagaag gtggaagaa gcgtggaag acgctcgag tttcaagtt atgttgcac aaggattcg aaggatgtt tatattttg ggttcgctg gaggaggtt cagagtgtt ttggatgat agtaccata aatataacg acaattgcg agttcgaga catgtgggt ccgattaaa tcacgtgtg gaggagtgg gcccgacag ttggatttg ttttcgagg acgctggat gaatgggtg gcttgtcaa cagagctgg ctttatttg gaggtgatt ttttccgcg cctgatatt caaagacag cttccgagc gagtcgaag ctttttatc attgtggac aagtcgtgg aagcagatt atgcgacgg accgcaaag atgccccta gctattgaa gccgctttc tatcccgac ttgctcgaa gagctccaa aaaaacaac gcacttttg gaacaaatc atgaagtgt ctcgaatcc tacttggaa gtgaaacgt gtggcattc cctcgtttc tacttccta tcaaacgac gaacttttg gagattttg gcccaaacc cgaaaccca cacgccgtc caaccccac ttgcgtaaa tgtttcgac gcaattgct cgtctagaa tttgcagca aaagaggaa gaagtcggc ataacacct ggcggtgac gaagatgtg aaaaaatcc aaatctgtg acgatgctc acgacaaat atttcggca atgatcagc cctgaaggc gaacgcgtg gcactgact aagggcttg aaagcgcga ggaaacgtc gaggattgg ctgggaaaa gtggaatcg tctatgttt ttcgctctg agacgtttg atgaaaggg gcactgatc gatttccag caaagtcgg agaattgaa tgggtggtg agacacccg agtcagatc accttgact gtgtcgcaa atcatgtgg gccagaggt gtccacgcg attttggac cacggaaac gcccacagt aatttggaa aagtttgag cagaaatgt atcggagat ttgaacgat ttggccact ttgattcgc tcggatttg gattcagtg actcgaaaa gtcttgatt gcgttaatt acgatcgat gtccatgcc agagatacc atcaggaac atggtcgag cacggagtc aaaaaaagc gatagtttc gactggttg aaagtcctg aggtactac tgggacgaa catcttgac gattgcgtt acaagaatg tcttcggct tactacatt tacggttac gagtatttg ggggcgtct ggtgtcctg gttataacc cccttaacc gaccgctgc tacctctgt ttgatgggt gccctccag ctggactta gggggcgct cctgcaggt ccagcggga actggcaaa accgaaacc actaaagat ttggccaaa gctttggcc attcagtgc gtggtgttt aactgttcg gaaggtttg gattataag attatgggt cggtttttt gccggattg gcacagtcc ggtgcttgg agttgtttc gatgagttt aatcgaatc gatattgag gtgctttcg gtgattgcg caacaaatt atcaccata cggaacgcc aaagctgcg aaattgaca cggttcatg tttgaggga cgcgaaatt aagctggtg cagaagtgt gccactttt attacgatg aatccggga tatgccgga aggactgaa cttccggat aatctgaag gctttgttt aggcctatt gcgatgatg gtccccgat tacgctctc atcgccgaa gtgattttg tattcggaa ggtttcgaa tcgtcaaaa gttctctct caaaaaatg gtgcaaatg tataagctt tgtagtgag caattgtcc caacaggat cactatgat tttgggatg cgtgccgtt aagagtgtc ttggtgatg gcaggctcc ctgaagagg gcaaacccc gaccgaaac gaggacgtg gtgttaatt tgtgcgttg cgcgacagt aatctaccg aaatttttg gccgacgat gctctcctt ttccaagga attttgagc gacttgttt cccggagtt gagttaccg gaacaggat tacggtaag ttccaggag gccatcgtg gacgtcatg gtgcaccaa cagctccaa ccggaaaaa cccatgatt cgaaaagtg attcaattg tacgaaacc atgattgtt cgatggggc gtgatgttg gtggggccc acagggggt gggaaaagc acagtttta aacactttg aataaagcc ttgacgaaa atgtacaat gatggtatc gaagggcct tattatcac ccagttcac acttatacc atgaaccca aaggctgta accgctggt gagttgtac ggagaggta aacatttat acgctggaa tggcgcgat ggtctcatg ggtatcatg atgcgaact gctgtgcaa tgtacggaa gaagaccac caatggatt atttgtgac ggtccagtg gatgctgtt tggattgaa aacttaaac accgttttg gacgacaat aaaatgttg tgtcttgca aattcggag cgtattaaa ctcacacct tacgtccat atggtgttc gaagtgatg gatctggca caggcttct ccagctact gtcagtcgt tgtggtatg gtctatata gaccctgat gaaattggc tggttgcct tatgccata tcgtgggtg caaagacgc gacgaagag cttcttaac cacgaattg aaacagttt atgattggt ttgtttgaa tatgctgtt gaaaatggt tttgctttt gtgaaaaaa aatggagat tactctata caccaagtg gatataagt aaagttgca atgctttgt gccatcatt gagagttat ttgaattca ccgaatgcg ttggaaaat atcggcgaa aagagcaaa gttaagagc tacttgtgt caagtgttt attttcgcc tatatttgg tcacttggc gggaatctt accgacgcg tcaaaggaa aagtttgaa gtttatgtg agggaacag tttgatgac catccagat gcaaggttg cctccagga gtcgacctc tacggcgta tttatgaac acgcaagac caccgcttg gacccatgg gcaaaaatc ctcccaacc ttcacctac aaacaagaa gtaccattt tttgaaatg ttggtacca accaacgac actgtccga tttggttac gtcatggaa cgtctaatg tacgtaaat tatcctgta ctattcgtc ggagatact ggtgttggc aaaacagtc gttgcaaaa gacgtcctt aaccgttta tatgaaacg ggtcaattt gttccggtg actataaat ttttcggct caaactagc agttttcga acacaggaa attctcgaa ttgaagctt gaaaaaaag aaaaaaact cttcttggt gccccattg ggcaaaaaa gtgttactt ttcgtcgat gatgtcaat atgcccaaa ttggagaca tacggggcc caacccccg attgaatta ctacgacag tttctaacc tatggtggt ttgtacgat cgggagaaa ttattttgg aaggaaatt cgcgatgtg attgtgtcg gcggcttgt gcaccccct gggggcggc aggaacccg ctcacgccc cgatttgtg cgatttttc gcaatgtta ctaatccca ccaccgaat gagttctcc ctcaaggcg atttttaag gcgatttta aaaggtttt ttctttgat ttttcgaac gaaatcaga gatttggcc gactatatg gtgggggct gccgttgaa atttacatg cggattgcg accgatttg ttgccaact cctgcaaaa tctcactac gtgttcaat ctacgggat ttatcgaaa tgtgtgcaa ggggtgctc caggcggat tctggcacc atgagggag gaaagtgca atgttgcgt ctgttctac cacgagtgt ttgcgcgtt tttcacgat cgattgatc aatgttgag gataaatcg tacttttac tttctgatg agggagatt tgtggtaga aatttcggt actgcggtt ctagccttg ccagatcag ccggttata accaatccg cctttgcta cttttcggc gactttatg caatacggc gccaataaa gaggaccgg atttatgag gaaattagg aacgttgac aagattcga agcgtttta caagattat ttggacgat tataatctt ctcgaatcg aaagacatg cggttgata tttttcatg gacgcgatt gaacattgt gttcgtatc gctcgaata ttgcgatct gagagggga aatgccctc cttgtgggg gttggagga atggggaag caaagtttg acgcgatta gcttcacac gtgaatgct tacaagtgt tttcaaatt gaactgacg cgaaactat gaccggtcg tactttttt gaggatttg agaaaaatg tattttaat gctggagcg aataatgca aattcggtt ttcctgttt accgatacg caaatcgtg caagaagat tttttggaa gatattaac aatattcta aattcgggc gaagtgccg aatcttttt gaggccgac gaatacgaa aaagtgata atagctacc agagatccg gcaaaaggt gcaggagtc gatcctgca aaccgtgac ggtatttac gactatttt ataagcaga gttcgcaat aatttgcat ttggtgatc tgtatgagt ccagtgggt gacgctttt aggcgccgt tgtcgcatg tttccctct ttggtcaat tgttgtaca attgattgg ttcgaaaaa tggccacat gaagcccta ttatctgtg tctcaaaat gctttaaaa gacctggga agtgaggaa ctttgccat aacttatcg acgatttgt gtgacgata cacgaaagt gtggaagaa atgactgaa cggttttat ctcgaaatg agacgacat tattacacg acaccgagt agctacctc gaattgttg aaactgtac cgaaaatta ctcgaaacg aaaaaggaa caagttatt tacaaacga tccagaatt agtaacgga ttacaaaaa ttgtacgaa acgaattca gttatcgaa acaatgaaa gaaactctg attgagctg gaaccagtt ttggccgaa aaatcggtc gctgtggac gaactaatg tcagatttg actacggaa caacaccaa gccgacaaa gtccgagct attgtcaaa tacgacgag gaaattgcc aaagcaaaa gccgaagat actcaagca ttggctgac gatgctcaa agagacctg gatacggca atgccggct ttggaagct gcaaccaaa gctcttgaa gcactcaac aaaaacgat atcaacgaa attaaagtg tttcaaaag ccgccgaaa cttgtacag tatgttatg gaatcggtt tgtctgcta ttaggtgcc aaaactgac tgggcctca gctaagatt gttctcggc gatgttaat ttcttaaag aagcttcag gagtacgac aagaatcac ataactgaa caaactcta cgtaagttg aagtcgtac gttgataat ccagatttt gttcctgat aaagtcggg agagtgtca aaagcgtgc aaaagcatg tgcatgtgg gtgcgtgcg atggatatg tatgcaaaa gtttacaaa attgtggag ccgaagaga aagagattg gaacaagcc gaaaaagag ctcaaccaa gtgatgggc cttttgcgc gagaaacag cgccaactc gccgaagtc gaagcaatg atcgcttca ctcgaagcc aaattcaac caaactctg gcggaaaaa gacgccctc gagaacgaa atggaatta acctccaac agattgaac cgtgccggc cgtttaaac gttgctctc ggcgacgaa caagctcga tgggagcgt tcagtcaaa gagttcgcc gtagaactc caaaacatc atcggcgat gctttaatt gccgctgct tgtgtggcc tatttgggc gcttttacc agcttgtat cgcaacgaa ttggtcgat ctatgggtc tcgcaattt aaagagttc caaataccg gcttcggat aatttcagt ttgattcga gttttggcc gatccgtac gatattcgc atgtggaac tctttcgga ctaccccgt gacactgtt tcgactgaa aatgcaatt ttggtcact caggctagt cgctgggcc cttatgatt gatccgcag gaacaagcc aatcgttgg attcgtcaa atggaagct gcgaacgat ttgcgagtg gtgaaactg accgattcg aattttttg cgcgttttg gagagcgcg attcgaatt ggtaaaccg gtgcttttg gaggaggtg ggcgagacg ttggatccg actttgggc ccgattttg accaaacag acgtttatg caggctgga cggcttttg atacgtttg ggggattcg gatgttgag tatgatccg aattttcga ttttatgtt acaacgaaa ttggcgaat ccgcattat ttgcccgaa atttgtatt caagtgacg attgttaat tttacggtg accaagtcg gggttggag gatcagttg ttagctgat gttgtgcgt ttagaacga cccgattta gagtcacaa agaacagaa ctgattata cgaatcaat aacgacaaa acacagttg caattaatc gaagataaa attttgaaa ttgttgtat cagtccgaa ggtaatatt ttggacgat gaagaatta attgagact ctaaatgag agcaaagaa acttcagct gtgattgcg gctcgtctt ttagagacc gaatcaacg gaacgtaaa atttcagaa gcgcgcgaa aaataccga actgtgtcc atcaggggc tcagttctt tatttcgtc attgcacaa ctggctgaa attgaccca atgtaccaa tactcactg aaatatttc aaccaaatc ttcaacaca gtgattgaa accagcgaa aaaagcaac gatcttaaa gaacggctc aagattttg ttgcgcgaa atcaccgca tttgtctac acaaacgtt tcaaggggt ttatttgag cgacacaaa ctggttttc agttttatg ctttgcgtt gcgattcac caagaaaag ggagaaatt agcgacaac caatggaat tttttgctc agaggtcca gtcggggcc aaaattgaa ttgccccca aaaccggat tatccccta attacggac gctatgtgg ctttcggcc aatttcttg gccgtttcg attccaggc tttgagaaa ttgccagaa gagatcaca aatgtgata agtgtcaga atttctgat ttccagcag gatatttcg gtggtgcca aatgccaag tcgagtcga gtcaaatgg aacgagctt ttggacgat tttaacaag ttgttgctt ttgaaaacc ttgaaagag gagaaattg gtgtttgcc atcaccgaa tatgttaaa atcaaactg gggaaacaa tttatcgaa agtccgcaa gtctcgctt caagttttg tatcaggac acgtcaaat ataactcct ttggtgttt atcctgagc acgggttca gatccgttt gggtcgttc caacgtttt gccgatgaa atgggtttt agggaaagg atcaagtcg atttcgctt ggacaagga caaggtcct gttgctgaa aagataatc gaacaagga ttggaacgt ggcgattgg gtctttctc caaaattgt catttggca acgtcttgg atgttggca atggaacgc ctggttata aaaattgcc gaacagtcg tccaaagtc cacaaggaa ttccgcctc tacatgagt tcaatgcca tcgaagtcg tttcccgtt tcagttttg caaaattcg gtcaaagtg accaacgag ccaccgaaa gggattagg gccaatatt aaaagggcc ttcaccgat atgcaacat gactttttc gaagatcat cctttaaag caagactgg agatgcatg attttcggt acttgcatg tttcatgca ataatacag gaacgaaaa aagtttggc cctttgggg tggaacatc acttatgag tttaacgac agcgatcgc gaatttgcg ttcaatact ttgaaaatg ttttgtgct gaagggacg atcccttgg gacgcttta gagtattta acaggggag attacctac ggtggacgt gttactgat tattgggac ttgaggtgt ttgaaaacg attttgaaa atcttcttc tcgcctcaa atacttaaa ccgcactat aagtactct cctagtggg atttattac tgccctagc tactctaaa cttgagaaa tatcaagag tttatcgac gggcttccg attttggaa gcaccggag attttcgga atgcatgaa aatgcgaac attgcctat caaatcaaa gagactcag aacatactt ttgacgatt atggaaagt caaccgcac acttctggc ggtgctgaa gggcaacaa accgataac attgtctac gatttggcg aatttggtc actgattct ttgatgacg aaaatctcg actgacgag gccaacgtc aacatgttt aaaagagac gacaagggc cgtttgccc tcactcaca acagtccta atgcaagaa gtcgaccgt tacaacatt ttactcaaa ctgatacac agttcgatg gataatttg aagaaagca atcaaaggt ttggtcgta atgtcggac gctttggag gaggtctac gttgcattt accaacaat caggtgcca aaaatgtgg aacaaaaaa gcctacaat tcgttaaaa agtctcgga agttggata cgagacttg gtgttgcgg ctcgatttt attaaaatt tgggtacga tcgggtcca ccgtcttcc tattggtta tcagggttt tatttccca caaggtttc ttaacgggg actttacaa acccatgcc agaaagtat aatttaccg atcgatcaa ctcaaattt gattttgac gtacaaaaa gtgtggatt gagcaggag caagtgaag aaaattcat gatgaggaa caacatgaa aatttggaa gtttataaa ggcttgcat catccggaa gatggggct ataattcat ggtctgttc ctggatgca ggtcgctgg gattccccg actcataaa ttggttgat gctaaacca ggtgaaatt aatccattg ttgccggcc atttggatg ttaccaaag acttcgtta cctcctaat gataagaga tatgtcact ccgttatac aaaacttca atacgtgca ggtgtcttg tccacaaca gggcacagc acaaatttt gtgattgct gtcttgtta ccaaccgat aaaccacaa agttattgg attcttaaa ggaactgca ttattaaca caagtcact gattgatta tcttttctt agttgtttt tgcaatgta ttgttgctt aagaataaa gtgtttttt tttaa
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