Status
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Gene information
General information
TC000117 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10955153..10957649
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC000117
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02978

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000117 RA
      MLPGIGLFG TGPMVKLLV PILRENGFK IEAVWSRTL 
HDAQETARE LNIPFFTNK IDDVLLRKD VDLIFVLCS 
PNLHAQISV KALGIGKHI VCDKPAGLN QCDALKMVR 
AGQYYPSLI SLINHPFRF LPALTHMRK AIKENYLES 
SITLIDIRI QSGSLFTDS DCFDWKCDD VMGGGALNL 
IGSHVIDLV TYLTNQRAV RVHGVVRTF TKTTPNITG 
IRHISAPDF CTFQMELGN GILVTATLN SHTMGGNFQ 
QEVMVCSTN GHLVVRGGD LFGRRNRGN EEVLYLDVE 
DLQCAAVVP NSSLPKPFI KGVSKMVSA LREAFLPVK 
EKAGWVKEP VCSAANFDD GLYVQAVLS AIRQSSNTR 
QWVKVDVLT KQPDTNALL SAAVRRTAI SMS
    CDS sequence
    • >TC000117 RA
      atgttacct ggaattggt ttatttggc accggccct 
atggtaaaa ctcctcgtg ccaattctc cgcgagaac 
ggcttcaaa atcgaagct gtgtggagc cgcactttg 
cacgacgct caagagaca gcccgtgag ctcaacatc 
cccttcttc accaacaaa atcgacgat gttctactt 
cgcaaagac gtcgatctg attttcgtt ttatgctcc 
ccgaattta cacgcccag atcagtgtt aaagccctg 
ggaatcggc aaacacatc gtttgcgac aagccagcg 
gggctcaac cagtgcgac gccctcaag atggtccga 
gctggccaa tactacccc tcgttgatt tccctcatc 
aaccacccg ttccgcttt ttacccgcc ttgacccac 
atgaggaaa gccatcaaa gagaattat ctcgagtca 
agcatcact ttgatcgat atcaggata cagagtggt 
tccttattc actgacagt gattgcttc gactggaag 
tgtgacgat gtgatgggg ggcggagcc ttgaacttg 
atcgggagc cacgtgatt gacctcgtg acttacttg 
accaatcag cgggcggtt cgtgtccat ggtgtggtt 
cgcacattc actaaaaca acaccaaat atcaccgga 
atcaggcac atctcagcc cctgatttt tgcacgttt 
caaatggag ctgggcaat ggtattctg gtgactgcg 
accctaaac agtcacacc atgggcggg aacttccag 
caggaggtg atggtgtgc agtaccaat gggcatttg 
gtggtccga gggggcgat ttgtttggg cgcaggaac 
cgagggaat gaggaagtg ttgtatttg gatgtggag 
gacttgcag tgtgctgcc gttgtgccc aattcaagt 
ctccccaag ccgtttatt aaaggggtt tcgaagatg 
gtgtctgcg ttgagggag gcgtttttg cccgttaaa 
gagaaggca ggttgggtt aaggagcct gtgtgttcg 
gctgctaat ttcgatgat gggttgtat gttcaggcc 
gttttgagt gcgattaga cagtcgtcg aatacgagg 
caatgggtt aaagttgat gttttgacg aagcaaccg 
gatacgaat gctttgttg agtgcggcg gttaggaga 
acagcaatt tccatgtcg tga
    mRNA sequence
    • >TC000117 RA
      ttggacttc ctatcaagt tgaattcgg acgcagtgt 
gcattattt gtaaactga cttcacaag gtgttaaac 
agcgtcgaa atgctataa taatctaca aaactgtat 
ccagctccg caaaatttt gaggttaga aaaatgtta 
cctggaatt ggtttattt ggcaccggc cctatggta 
aaactcctc gtgccaatt ctccgcgag aacggcttc 
aaaatcgaa gctgtgtgg agccgcact ttgcacgac 
gctcaagag acagcccgt gagctcaac atccccttc 
ttcaccaac aaaatcgac gatgttcta cttcgcaaa 
gacgtcgat ctgattttc gttttatgc tccccgaat 
ttacacgcc cagatcagt gttaaagcc ctgggaatc 
ggcaaacac atcgtttgc gacaagcca gcggggctc 
aaccagtgc gacgccctc aagatggtc cgagctggc 
caatactac ccctcgttg atttccctc atcaaccac 
ccgttccgc tttttaccc gccttgacc cacatgagg 
aaagccatc aaagagaat tatctcgag tcaagcatc 
actttgatc gatatcagg atacagagt ggttcctta 
ttcactgac agtgattgc ttcgactgg aagtgtgac 
gatgtgatg gggggcgga gccttgaac ttgatcggg 
agccacgtg attgacctc gtgacttac ttgaccaat 
cagcgggcg gttcgtgtc catggtgtg gttcgcaca 
ttcactaaa acaacacca aatatcacc ggaatcagg 
cacatctca gcccctgat ttttgcacg tttcaaatg 
gagctgggc aatggtatt ctggtgact gcgacccta 
aacagtcac accatgggc gggaacttc cagcaggag 
gtgatggtg tgcagtacc aatgggcat ttggtggtc 
cgagggggc gatttgttt gggcgcagg aaccgaggg 
aatgaggaa gtgttgtat ttggatgtg gaggacttg 
cagtgtgct gccgttgtg cccaattca agtctcccc 
aagccgttt attaaaggg gtttcgaag atggtgtct 
gcgttgagg gaggcgttt ttgcccgtt aaagagaag 
gcaggttgg gttaaggag cctgtgtgt tcggctgct 
aatttcgat gatgggttg tatgttcag gccgttttg 
agtgcgatt agacagtcg tcgaatacg aggcaatgg 
gttaaagtt gatgttttg acgaagcaa ccggatacg 
aatgctttg ttgagtgcg gcggttagg agaacagca 
atttccatg tcgtgaacg ggcgactgt tgtttggac 
tggggcggg ttaagtacc tggcggtcc aagggtaca 
tgttgaaaa catccataa cagaagaaa ttaatagtg 
tattatact cgtattata agaatagct ttatgagca 
gatgctgat aaaaaaatc agttaaagc acatactta 
ctgcttcaa aaccacgga gatgccaat gtcgcattt 
taccgaatt atttttttt aataagggt gataaaaag 
taaaagcta tttatgatt agatttttc attaaaact 
ataaataca ttagctgtt aattttttg aagtgcatg 
aatataatt tataacaac taactttta gagaaaatg 
cggcgttgg catttccat agttctgaa acagagagt 
atagcgctt atactctga ataatacat gagggtgcc 
ccagattaa gtataggag ccgtgaagc acatgttct 
tgtgtgaga tctaataac aaaatcgaa agaaaaaaa 
tccagggtc aaatagttt tgcagttat aaataaacg 
ttatatact gaccaatca tgaagcact tgcaattaa 
attttgtag gtgtaatct gaaaactca ctttgttac 
cgaagtaac gaacaaaac ttaaatgat ttttataac 
aattcattt ttataagag tagctattt ctatttccc 
agttttgtg ataatattg agctgcaag gtaaggtat 
ttttaataa gagctcttg tattggtca acttatact 
gtttataac tcaaaaaca agacctgga aattttttc 
tttcaacat tgttattaa atctcacgc gtgagttta 
tattttcct acactttat caggacccc tctgtatac 
tatactttt ttccattgt gttagaaaa tcacaattc 
ctgattgac aaacacttt acattctaa acaacattt 
aatttaatg ctcctctag attcgatta tgaaactgg 
cccttagct tcttgatct gcaaaaatg atttaaacg 
caatccgag gtgaattta ctcgagtca aaagatttt 
tctcagtgt taaaatgtg ttaataaaa gtatttaag 
aaacgcgat agaacccag ataccaaca agaaatttt 
agacggaag aaatactga ataattgat gattttctt 
a

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY