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Gene information
General information
TC000117 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10955153..10957649
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000117
    iBeetle screen iB_02978

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000117 RA
      MLPGIGLFG TGPMVKLLV PILRENGFK IEAVWSRTL 
      HDAQETARE LNIPFFTNK IDDVLLRKD VDLIFVLCS 
      PNLHAQISV KALGIGKHI VCDKPAGLN QCDALKMVR 
      AGQYYPSLI SLINHPFRF LPALTHMRK AIKENYLES 
      SITLIDIRI QSGSLFTDS DCFDWKCDD VMGGGALNL 
      IGSHVIDLV TYLTNQRAV RVHGVVRTF TKTTPNITG 
      IRHISAPDF CTFQMELGN GILVTATLN SHTMGGNFQ 
      QEVMVCSTN GHLVVRGGD LFGRRNRGN EEVLYLDVE 
      DLQCAAVVP NSSLPKPFI KGVSKMVSA LREAFLPVK 
      EKAGWVKEP VCSAANFDD GLYVQAVLS AIRQSSNTR 
      QWVKVDVLT KQPDTNALL SAAVRRTAI SMS
    CDS sequence
    • >TC000117 RA
      atgttacct ggaattggt ttatttggc accggccct 
      atggtaaaa ctcctcgtg ccaattctc cgcgagaac 
      ggcttcaaa atcgaagct gtgtggagc cgcactttg 
      cacgacgct caagagaca gcccgtgag ctcaacatc 
      cccttcttc accaacaaa atcgacgat gttctactt 
      cgcaaagac gtcgatctg attttcgtt ttatgctcc 
      ccgaattta cacgcccag atcagtgtt aaagccctg 
      ggaatcggc aaacacatc gtttgcgac aagccagcg 
      gggctcaac cagtgcgac gccctcaag atggtccga 
      gctggccaa tactacccc tcgttgatt tccctcatc 
      aaccacccg ttccgcttt ttacccgcc ttgacccac 
      atgaggaaa gccatcaaa gagaattat ctcgagtca 
      agcatcact ttgatcgat atcaggata cagagtggt 
      tccttattc actgacagt gattgcttc gactggaag 
      tgtgacgat gtgatgggg ggcggagcc ttgaacttg 
      atcgggagc cacgtgatt gacctcgtg acttacttg 
      accaatcag cgggcggtt cgtgtccat ggtgtggtt 
      cgcacattc actaaaaca acaccaaat atcaccgga 
      atcaggcac atctcagcc cctgatttt tgcacgttt 
      caaatggag ctgggcaat ggtattctg gtgactgcg 
      accctaaac agtcacacc atgggcggg aacttccag 
      caggaggtg atggtgtgc agtaccaat gggcatttg 
      gtggtccga gggggcgat ttgtttggg cgcaggaac 
      cgagggaat gaggaagtg ttgtatttg gatgtggag 
      gacttgcag tgtgctgcc gttgtgccc aattcaagt 
      ctccccaag ccgtttatt aaaggggtt tcgaagatg 
      gtgtctgcg ttgagggag gcgtttttg cccgttaaa 
      gagaaggca ggttgggtt aaggagcct gtgtgttcg 
      gctgctaat ttcgatgat gggttgtat gttcaggcc 
      gttttgagt gcgattaga cagtcgtcg aatacgagg 
      caatgggtt aaagttgat gttttgacg aagcaaccg 
      gatacgaat gctttgttg agtgcggcg gttaggaga 
      acagcaatt tccatgtcg tga
    mRNA sequence
    • >TC000117 RA
      ttggacttc ctatcaagt tgaattcgg acgcagtgt 
      gcattattt gtaaactga cttcacaag gtgttaaac 
      agcgtcgaa atgctataa taatctaca aaactgtat 
      ccagctccg caaaatttt gaggttaga aaaatgtta 
      cctggaatt ggtttattt ggcaccggc cctatggta 
      aaactcctc gtgccaatt ctccgcgag aacggcttc 
      aaaatcgaa gctgtgtgg agccgcact ttgcacgac 
      gctcaagag acagcccgt gagctcaac atccccttc 
      ttcaccaac aaaatcgac gatgttcta cttcgcaaa 
      gacgtcgat ctgattttc gttttatgc tccccgaat 
      ttacacgcc cagatcagt gttaaagcc ctgggaatc 
      ggcaaacac atcgtttgc gacaagcca gcggggctc 
      aaccagtgc gacgccctc aagatggtc cgagctggc 
      caatactac ccctcgttg atttccctc atcaaccac 
      ccgttccgc tttttaccc gccttgacc cacatgagg 
      aaagccatc aaagagaat tatctcgag tcaagcatc 
      actttgatc gatatcagg atacagagt ggttcctta 
      ttcactgac agtgattgc ttcgactgg aagtgtgac 
      gatgtgatg gggggcgga gccttgaac ttgatcggg 
      agccacgtg attgacctc gtgacttac ttgaccaat 
      cagcgggcg gttcgtgtc catggtgtg gttcgcaca 
      ttcactaaa acaacacca aatatcacc ggaatcagg 
      cacatctca gcccctgat ttttgcacg tttcaaatg 
      gagctgggc aatggtatt ctggtgact gcgacccta 
      aacagtcac accatgggc gggaacttc cagcaggag 
      gtgatggtg tgcagtacc aatgggcat ttggtggtc 
      cgagggggc gatttgttt gggcgcagg aaccgaggg 
      aatgaggaa gtgttgtat ttggatgtg gaggacttg 
      cagtgtgct gccgttgtg cccaattca agtctcccc 
      aagccgttt attaaaggg gtttcgaag atggtgtct 
      gcgttgagg gaggcgttt ttgcccgtt aaagagaag 
      gcaggttgg gttaaggag cctgtgtgt tcggctgct 
      aatttcgat gatgggttg tatgttcag gccgttttg 
      agtgcgatt agacagtcg tcgaatacg aggcaatgg 
      gttaaagtt gatgttttg acgaagcaa ccggatacg 
      aatgctttg ttgagtgcg gcggttagg agaacagca 
      atttccatg tcgtgaacg ggcgactgt tgtttggac 
      tggggcggg ttaagtacc tggcggtcc aagggtaca 
      tgttgaaaa catccataa cagaagaaa ttaatagtg 
      tattatact cgtattata agaatagct ttatgagca 
      gatgctgat aaaaaaatc agttaaagc acatactta 
      ctgcttcaa aaccacgga gatgccaat gtcgcattt 
      taccgaatt atttttttt aataagggt gataaaaag 
      taaaagcta tttatgatt agatttttc attaaaact 
      ataaataca ttagctgtt aattttttg aagtgcatg 
      aatataatt tataacaac taactttta gagaaaatg 
      cggcgttgg catttccat agttctgaa acagagagt 
      atagcgctt atactctga ataatacat gagggtgcc 
      ccagattaa gtataggag ccgtgaagc acatgttct 
      tgtgtgaga tctaataac aaaatcgaa agaaaaaaa 
      tccagggtc aaatagttt tgcagttat aaataaacg 
      ttatatact gaccaatca tgaagcact tgcaattaa 
      attttgtag gtgtaatct gaaaactca ctttgttac 
      cgaagtaac gaacaaaac ttaaatgat ttttataac 
      aattcattt ttataagag tagctattt ctatttccc 
      agttttgtg ataatattg agctgcaag gtaaggtat 
      ttttaataa gagctcttg tattggtca acttatact 
      gtttataac tcaaaaaca agacctgga aattttttc 
      tttcaacat tgttattaa atctcacgc gtgagttta 
      tattttcct acactttat caggacccc tctgtatac 
      tatactttt ttccattgt gttagaaaa tcacaattc 
      ctgattgac aaacacttt acattctaa acaacattt 
      aatttaatg ctcctctag attcgatta tgaaactgg 
      cccttagct tcttgatct gcaaaaatg atttaaacg 
      caatccgag gtgaattta ctcgagtca aaagatttt 
      tctcagtgt taaaatgtg ttaataaaa gtatttaag 
      aaacgcgat agaacccag ataccaaca agaaatttt 
      agacggaag aaatactga ataattgat gattttctt 
      a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY