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Gene information
General information
TC000113 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10983996..10988560
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000113
    iBeetle screen iB_02977

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000113 RA
      MALCEYNNL KEWAPLTDL FSKLPTKVQ NGLFTQDVK 
      LTCLDVLPE FLALGTNVG LVYWYDRKK KDLQRLRCE 
      KQNVQITVV RIISTVDFM VACGSTDGC INIFQIPKS 
      HPDSIPDNL KPKSKQVER FTVSDLHKC PVTALEWSK 
      NGMKLFSGD KNGCIVLTE IDFYMHICK SAEILNESY 
      QVIQLSYSQ QRLLVSTTY RSIICQRLD KWKVCQVGK 
      KDRKILGPF GGLIHQNGP KTTDAVLYC TRPGLRIWV 
      ADCDGNVNK TLLFKDLLK KECSEVPII NPISRNLKM 
      LKPLKEPSF GVLLPFSEN LILTYNNDV IYVLDPEQM 
      TIISTMSHF RSVLGVATH KDEIFILEE DRSVVRISY 
      KPEQIPDCD QNSASPVAS MFLPITTSL KDITTKLQT 
      GNILPDVNI HTDESLIMN AEEAKESPI KTRANPEIE 
      KKFNDIGRQ QFEEKILYR QSNKHRKRM CASMSSLSS 
      NSSEEKEGI KPTLMTLSS VGVLPDLRS PESIQNDIE 
      YKERILADM LSLDTIKIS LDQLQENNE SCDDDLINE 
      PTSSNPEHI PEISDEVVK DSSPLQSSK KQIVHNEAP 
      PVCLNIPND WKLADICLN VKTEPEVIP KNDTSLTDN 
      SLADWEIV
    CDS sequence
    • >TC000113 RA
      atggccctt tgtgagtac aacaatttg aaagagtgg 
      gcccctttg accgacttg ttcagcaag ttgcctacg 
      aaagtccag aacgggttg ttcactcaa gacgtgaag 
      ttgacctgt ttggacgtt ttgcccgaa tttttggcg 
      ttggggacg aacgtgggt ctcgtctat tggtacgat 
      aggaaaaag aaggatttg caacgactt cgttgtgag 
      aaacaaaac gtacaaata actgtggtt cggatcata 
      tcaactgtt gattttatg gtggcttgt gggagcact 
      gatgggtgt attaatatt tttcaaatt cctaaatcc 
      caccctgac tcgattccg gataatttg aagccgaaa 
      agtaagcaa gtggagagg tttacggtg tctgacttg 
      cacaaatgt ccagtgacg gcgctcgag tggtctaaa 
      aacggaatg aaactcttc tcaggtgat aaaaacgga 
      tgcattgtt ttaacagaa attgacttc tatatgcac 
      atttgtaaa tcagcggaa attctaaac gaatcgtat 
      caagtaatc caactcagt tacagccaa caacgactc 
      ctagtctca acaacttac agaagtatc atttgccaa 
      agactggac aagtggaaa gtgtgtcaa gtgggcaag 
      aaagacagg aaaatttta ggccctttc ggagggcta 
      attcatcaa aacggccct aaaactacc gatgcggtt 
      ttatattgc acgagaccg gggcttcga atatgggtg 
      gctgattgt gacggtaat gtcaataaa actctctta 
      tttaaggat ctgttgaaa aaagaatgc tcagaagtg 
      ccaataatt aacccgatt tcgcggaat ttaaaaatg 
      ctcaagccg ttgaaggaa ccctcattt ggtgtcctg 
      cttccgttt agtgaaaat ttaattcta acgtacaat 
      aatgacgtg atttatgtc ctagatccg gaacaaatg 
      acaattatt tcaaccatg agccatttt cgcagtgtt 
      ttgggtgtg gcgacccac aaagacgag attttcatt 
      ctggaagag gaccgaagt gtcgtccga atctcctat 
      aaaccggaa caaatcccc gattgtgat caaaactcg 
      gcttcgcct gtcgctagt atgtttctt ccgatcaca 
      acatcgctt aaagatatc acaactaag ctccaaacc 
      ggaaatatc ctcccggat gttaacatc cacactgac 
      gaatctctc ataatgaac gccgaggaa gcgaaggaa 
      tcgcccata aaaacacgt gctaacccc gaaattgag 
      aaaaaattc aacgatatt ggacgacaa caattcgag 
      gagaaaatc ctgtacagg caaagtaat aagcaccga 
      aagcgcatg tgtgcgagt atgagtagt ttgagttcg 
      aatagttcg gaggagaag gaagggatc aaaccgact 
      ctgatgact ttgagttcg gtcggggtt ttacccgat 
      ttgaggagt ccagagtct atacaaaac gatatcgaa 
      tacaaagag cgaattctt gccgatatg ctgagtcta 
      gatacgatt aagatatca ttagatcaa ttacaagag 
      aataatgag agctgtgat gatgattta atcaatgaa 
      ccaacttca agtaatcct gaacacatt ccagaaatt 
      agtgatgaa gttgtaaaa gattctagc ccactacaa 
      agtagtaaa aaacaaata gtacacaac gaagcacct 
      cctgtatgt ttaaacatt ccgaatgac tggaagttg 
      gccgatatt tgtctaaat gttaaaacc gaaccggaa 
      gtaattccg aaaaatgat acgtcgctg actgataat 
      tcgttagcg gattgggaa atcgtctaa
    mRNA sequence
    • >TC000113 RA
      tgatgtaag aactgtcat tgtcaaaaa atcacggaa 
      tgttttatt tctaaaaat tccgttttt gtaattgag 
      ataagccgt gctgtttgt tttgtttat tgttatgcc 
      ggcgcggca cgtttcggc acaatggcc ctttgtgag 
      tacaacaat ttgaaagag tgggcccct ttgaccgac 
      ttgttcagc aagttgcct acgaaagtc cagaacggg 
      ttgttcact caagacgtg aagttgacc tgtttggac 
      gttttgccc gaatttttg gcgttgggg acgaacgtg 
      ggtctcgtc tattggtac gataggaaa aagaaggat 
      ttgcaacga cttcgttgt gagaaacaa aacgtacaa 
      ataactgtg gttcggatc atatcaact gttgatttt 
      atggtggct tgtgggagc actgatggg tgtattaat 
      atttttcaa attcctaaa tcccaccct gactcgatt 
      ccggataat ttgaagccg aaaagtaag caagtggag 
      aggtttacg gtgtctgac ttgcacaaa tgtccagtg 
      acggcgctc gagtggtct aaaaacgga atgaaactc 
      ttctcaggt gataaaaac ggatgcatt gttttaaca 
      gaaattgac ttctatatg cacatttgt aaatcagcg 
      gaaattcta aacgaatcg tatcaagta atccaactc 
      agttacagc caacaacga ctcctagtc tcaacaact 
      tacagaagt atcatttgc caaagactg gacaagtgg 
      aaagtgtgt caagtgggc aagaaagac aggaaaatt 
      ttaggccct ttcggaggg ctaattcat caaaacggc 
      cctaaaact accgatgcg gttttatat tgcacgaga 
      ccggggctt cgaatatgg gtggctgat tgtgacggt 
      aatgtcaat aaaactctc ttatttaag gatctgttg 
      aaaaaagaa tgctcagaa gtgccaata attaacccg 
      atttcgcgg aatttaaaa atgctcaag ccgttgaag 
      gaaccctca tttggtgtc ctgcttccg tttagtgaa 
      aatttaatt ctaacgtac aataatgac gtgatttat 
      gtcctagat ccggaacaa atgacaatt atttcaacc 
      atgagccat tttcgcagt gttttgggt gtggcgacc 
      cacaaagac gagattttc attctggaa gaggaccga 
      agtgtcgtc cgaatctcc tataaaccg gaacaaatc 
      cccgattgt gatcaaaac tcggcttcg cctgtcgct 
      agtatgttt cttccgatc acaacatcg cttaaagat 
      atcacaact aagctccaa accggaaat atcctcccg 
      gatgttaac atccacact gacgaatct ctcataatg 
      aacgccgag gaagcgaag gaatcgccc ataaaaaca 
      cgtgctaac cccgaaatt gagaaaaaa ttcaacgat 
      attggacga caacaattc gaggagaaa atcctgtac 
      aggcaaagt aataagcac cgaaagcgc atgtgtgcg 
      agtatgagt agtttgagt tcgaatagt tcggaggag 
      aaggaaggg atcaaaccg actctgatg actttgagt 
      tcggtcggg gttttaccc gatttgagg agtccagag 
      tctatacaa aacgatatc gaatacaaa gagcgaatt 
      cttgccgat atgctgagt ctagatacg attaagata 
      tcattagat caattacaa gagaataat gagagctgt 
      gatgatgat ttaatcaat gaaccaact tcaagtaat 
      cctgaacac attccagaa attagtgat gaagttgta 
      aaagattct agcccacta caaagtagt aaaaaacaa 
      atagtacac aacgaagca cctcctgta tgtttaaac 
      attccgaat gactggaag ttggccgat atttgtcta 
      aatgttaaa accgaaccg gaagtaatt ccgaaaaat 
      gatacgtcg ctgactgat aattcgtta gcggattgg 
      gaaatcgtc taaagtgag tcagttatc ttgttaatg 
      attatattt gagttgtca taaacaaat cgtgggctt 
      actgcaaaa agtggccaa actggccat taaaaaaaa 
      tattttttt acatttatt gataccttt tcatgctta 
      ttttcattt aaatgcaca ttagtaatt atttctgaa 
      aggatctta agaggaaat ggcaaagac gcatttgca 
      tgttttgga gtgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY