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Gene information
General information
TC000097 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 6751955..6754939
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000097
    iBeetle screen iB_02974
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000097 RA
      MFVASLCIL ASLISTSSP YCVEEWDEI YVIHCTNTS 
      LENFIYDFD PFNEKDSQY RRSYAIVIT NSQLKRIDF 
      FYLPFSNPS NLMRLSLTN SSIEYINDS AFEDYRFSD 
      LVLSNNKLH SMSFVEKLP TNLNKLYLD GNHFSSVGN 
      VFANLIHLT HLDLSNCRI QNIDPDSFS SLKSLIFIN 
      VSNNRLVRG FFVFNFSPI PFFDLSFNK MEHLDFKGD 
      NLHFGELDI SHNPLKNID FLNKIKFGD LKLASTNVS 
      GDFNFLLLN NVYFSLDLN NNKLVNLHS DLFKVETYK 
      GPFRKNINF FNSSISNID RHAFKNFGY YYDYSPVDN 
      NPPILNLSS NAITELKNY TFEGSFIST LDLSKCLIQ 
      NIQIKAFSG LYLTDTLNL SLNRISHLN ENVFKNLTL 
      VQTIDLSFN FLKKLNNQL FIDCHSLKF INLSNSRIE 
      LIKEWAFAG SDNLLELNL ENNFLEKIN FRNFSRLEL 
      LNLHNNKLS NLDLSFLCN LSAKNVVMS HNKIGKLLN 
      NSCATVKSP QEMDLSKSS ICSIEAKTF RGLFWLEVL 
      TLRDNFIET ILPETFTYL KRLKQLDLS ANRIAFLSE 
      NAFAFGNRL NMLNITFVN ANLTGHLFK WLNHVVTLN 
      ISNSQVSLL KGCFKGLKY LENFYFHKS EVVEVIPGA 
      FQGLDSLKQ FNVHSIFRN TKVLQSGTF EGLKYLDHL 
      NLSHLEITV IETQAFEGL TTLLKLCLS RNNLTKIIS 
      GTFVGLDHL KMLDLSRNK ILQLEVRAF YGLYSLREL 
      HLNRNNLTT LSLGTFQQF PTLEVLNLR RNNINKILT 
      GTFSNLKFL KTLDLSYNN ITKLKMETL ITLSNLKKI 
      DLTANHLIQ MEHLDLIKS LRHLEFVGL NDNKWKCDN 
      LVSMLIAFQ NYSVNWLST ESATFTHEN IDGIKCFDI 
      CNFFYCVDE EDLTWRN
    CDS sequence
    • >TC000097 RA
      atgtttgtg gcaagcttg tgcatcctg gcatcactt 
      atctcaact tcgagcccg tattgcgtt gaagagtgg 
      gacgaaatc tacgtaatt cattgcacg aacacaagt 
      cttgaaaat ttcatttac gactttgat ccgttcaac 
      gagaaagac tcccagtat cgaagaagc tacgccatt 
      gtaatcacc aattcacaa ttgaaacga atcgacttt 
      ttctacctc cccttttcg aatccttca aatctgatg 
      cgactgtct ttaaccaac tcttctatc gagtacatc 
      aacgattca gctttcgag gattatcgt ttcagcgat 
      ttggttttg agcaacaac aaattacac agtatgtcg 
      tttgtcgaa aaattacca acaaattta aacaaactg 
      tatttggac ggaaaccat ttcagctca gttggaaat 
      gttttcgcc aatttaatt cacttgact cacctagat 
      ttaagcaac tgtcgaata caaaacatc gatccggac 
      agtttttcc agcttgaaa agtttgatc tttatcaat 
      gttagtaac aacagactt gtgcgaggt tttttcgta 
      tttaacttc tccccaatt ccgtttttc gacctgagt 
      tttaacaaa atggaacac cttgatttc aaaggcgat 
      aatttacat tttggcgaa ttggatatt agccacaac 
      cctttgaaa aatatcgat tttttgaac aaaattaaa 
      tttggcgat ttaaaacta gcaagtact aatgtaagt 
      ggcgacttt aattttcta ttactcaac aatgtgtac 
      ttttcgttg gatttaaac aacaataag ctggttaat 
      ttacattcg gacttgttt aaagtggag acgtataaa 
      ggccccttc aggaaaaac attaatttc tttaattct 
      tcaatcagc aacattgac agacatgcg tttaaaaac 
      ttcggttac tattatgat tactcaccc gttgataat 
      aacccacca attctcaat cttagttca aacgcgatc 
      accgaactc aaaaattac acttttgag ggcagtttt 
      attagcact ttggacctc tcaaagtgt ttgattcaa 
      aatatacaa atcaaggct ttctccggt ttatatcta 
      accgacact ttaaactta agcctcaac cgaatatca 
      cacttaaac gaaaacgtt ttcaaaaac ttgactctt 
      gtccaaacg attgatttg tcgttcaat ttcctgaaa 
      aaactcaac aatcagtta tttatcgac tgtcacagt 
      ttgaaattt ataaactta tccaattcc aggattgag 
      ttaataaaa gaatgggcc tttgccgga agtgataat 
      ttactagaa ctaaacctt gaaaataat tttttggag 
      aaaatcaat tttcgtaat ttcagtcgc ctcgaattg 
      ctaaacctg cacaacaat aaactttca aatttagat 
      ttgtcgttt ctgtgtaat ttatcggcg aaaaatgtg 
      gtcatgtca cataataaa ataggaaaa ttattaaac 
      aactcttgt gcaactgta aaatcgcca caagaaatg 
      gatttatcc aaatcgtca atttgtagc atcgaagcg 
      aaaacattt cgtggtttg ttttggttg gaagttctc 
      actttgagg gacaacttt atcgaaaca attttaccc 
      gaaactttc acgtatttg aagcgatta aaacaactc 
      gatctttcc gccaacaga attgcgttt ttatcggag 
      aatgcgttc gcttttgga aaccgtctc aatatgttg 
      aatattacg ttcgttaat gcaaacttg actggtcat 
      ttatttaag tggttgaat catgtcgtg actttgaac 
      atttccaat tcgcaagtg agtttgttg aagggttgc 
      ttcaaaggt ctcaaatac ttggagaat ttttatttc 
      cataaaagt gaagttgtg gaggtaatt cctggtgca 
      tttcagggt ctagacagt ttgaaacaa ttcaacgta 
      cactcaatt tttcgaaac accaaagtt ttgcaaagt 
      gggaccttt gaaggtttg aaatatttg gatcatttg 
      aatttgtcg catcttgaa ataacggtg attgaaact 
      caagctttt gaaggcctt acaactctt ctcaaattg 
      tgtttgagt cgaaataat ttgaccaaa attataagt 
      gggactttt gtgggtttg gaccatttg aaaatgtta 
      gatttgtcg aggaataaa attttgcag ttggaagtg 
      cgggccttt tatggcttg tattcactg cgtgaatta 
      cacttgaat cggaataat ttaacgact ttgtctctc 
      ggaactttt caacaattc cctacgttg gaagtgttg 
      aatttgcgc aggaataac ataaataaa attctgact 
      gggactttt tccaatttg aaatttcta aaaactttg 
      gatttgtcg tataataac atcacgaaa cttaaaatg 
      gaaacgctt attacgttg agcaatttg aaaaagatt 
      gatttgact gcgaatcat ttgattcag atggaacat 
      ttggatttg atcaagtct ttgagacac ttggagttt 
      gtaggtctt aatgataac aagtggaag tgcgataat 
      ttggtgtca atgttgatc gcgtttcaa aattatagc 
      gtcaactgg ttgtctact gaaagtgca acttttacg 
      catgaaaat atagacggg attaagtgt tttgatatt 
      tgtaatttc ttttattgt gttgacgag gaagacctc 
      acttggagg aattga
    mRNA sequence
    • >TC000097 RA
      gagatcaaa tatcttgtt ccgtttccg gtgatacac 
      cgaaatttt tgtgtgtca gtgaaaaat aaattaaaa 
      tgtgtgaaa acaaattta attagtgca acgtgtaaa 
      gtttttgta acaagtata tcgtcttgc agacagacc 
      aaaaatgtt tgtggcaag cttgtgcat cctggcatc 
      acttatctc aacttcgag cccgtattg cgttgaaga 
      gtgggacga aatctacgt aattcattg cacgaacac 
      aagtcttga aaatttcat ttacgactt tgatccgtt 
      caacgagaa agactccca gtatcgaag aagctacgc 
      cattgtaat caccaattc acaattgaa acgaatcga 
      ctttttcta cctcccctt ttcgaatcc ttcaaatct 
      gatgcgact gtctttaac caactcttc tatcgagta 
      catcaacga ttcagcttt cgaggatta tcgtttcag 
      cgatttggt tttgagcaa caacaaatt acacagtat 
      gtcgtttgt cgaaaaatt accaacaaa tttaaacaa 
      actgtattt ggacggaaa ccatttcag ctcagttgg 
      aaatgtttt cgccaattt aattcactt gactcacct 
      agatttaag caactgtcg aatacaaaa catcgatcc 
      ggacagttt ttccagctt gaaaagttt gatctttat 
      caatgttag taacaacag acttgtgcg aggtttttt 
      cgtatttaa cttctcccc aattccgtt tttcgacct 
      gagttttaa caaaatgga acaccttga tttcaaagg 
      cgataattt acattttgg cgaattgga tattagcca 
      caacccttt gaaaaatat cgatttttt gaacaaaat 
      taaatttgg cgatttaaa actagcaag tactaatgt 
      aagtggcga ctttaattt tctattact caacaatgt 
      gtacttttc gttggattt aaacaacaa taagctggt 
      taatttaca ttcggactt gtttaaagt ggagacgta 
      taaaggccc cttcaggaa aaacattaa tttctttaa 
      ttcttcaat cagcaacat tgacagaca tgcgtttaa 
      aaacttcgg ttactatta tgattactc acccgttga 
      taataaccc accaattct caatcttag ttcaaacgc 
      gatcaccga actcaaaaa ttacacttt tgagggcag 
      ttttattag cactttgga cctctcaaa gtgtttgat 
      tcaaaatat acaaatcaa ggctttctc cggtttata 
      tctaaccga cactttaaa cttaagcct caaccgaat 
      atcacactt aaacgaaaa cgttttcaa aaacttgac 
      tcttgtcca aacgattga tttgtcgtt caatttcct 
      gaaaaaact caacaatca gttatttat cgactgtca 
      cagtttgaa atttataaa cttatccaa ttccaggat 
      tgagttaat aaaagaatg ggcctttgc cggaagtga 
      taatttact agaactaaa ccttgaaaa taatttttt 
      ggagaaaat caattttcg taatttcag tcgcctcga 
      attgctaaa cctgcacaa caataaact ttcaaattt 
      agatttgtc gtttctgtg taatttatc ggcgaaaaa 
      tgtggtcat gtcacataa taaaatagg aaaattatt 
      aaacaactc ttgtgcaac tgtaaaatc gccacaaga 
      aatggattt atccaaatc gtcaatttg tagcatcga 
      agcgaaaac atttcgtgg tttgttttg gttggaagt 
      tctcacttt gagggacaa ctttatcga aacaatttt 
      acccgaaac tttcacgta tttgaagcg attaaaaca 
      actcgatct ttccgccaa cagaattgc gtttttatc 
      ggagaatgc gttcgcttt tggaaaccg tctcaatat 
      gttgaatat tacgttcgt taatgcaaa cttgactgg 
      tcatttatt taagtggtt gaatcatgt cgtgacttt 
      gaacatttc caattcgca agtgagttt gttgaaggg 
      ttgcttcaa aggtctcaa atacttgga gaattttta 
      tttccataa aagtgaagt tgtggaggt aattcctgg 
      tgcatttca gggtctaga cagtttgaa acaattcaa 
      cgtacactc aatttttcg aaacaccaa agttttgca 
      aagtgggac ctttgaagg tttgaaata tttggatca 
      tttgaattt gtcgcatct tgaaataac ggtgattga 
      aactcaagc ttttgaagg ccttacaac tcttctcaa 
      attgtgttt gagtcgaaa taatttgac caaaattat 
      aagtgggac ttttgtggg tttggacca tttgaaaat 
      gttagattt gtcgaggaa taaaatttt gcagttgga 
      agtgcgggc cttttatgg cttgtattc actgcgtga 
      attacactt gaatcggaa taatttaac gactttgtc 
      tctcggaac ttttcaaca attccctac gttggaagt 
      gttgaattt gcgcaggaa taacataaa taaaattct 
      gactgggac tttttccaa tttgaaatt tctaaaaac 
      tttggattt gtcgtataa taacatcac gaaacttaa 
      aatggaaac gcttattac gttgagcaa tttgaaaaa 
      gattgattt gactgcgaa tcatttgat tcagatgga 
      acatttgga tttgatcaa gtctttgag acacttgga 
      gtttgtagg tcttaatga taacaagtg gaagtgcga 
      taatttggt gtcaatgtt gatcgcgtt tcaaaatta 
      tagcgtcaa ctggttgtc tactgaaag tgcaacttt 
      tacgcatga aaatataga cgggattaa gtgttttga 
      tatttgtaa tttctttta ttgtgttga cgaggaaga 
      cctcacttg gaggaattg aattatcca aggtttata 
      ataaatatt ttagtgtta t