| General information | |||
| TC000097 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 11313242..11316226 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC000097 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02974 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC000097 RA
MFVASLCIL ASLISTSSP YCVEEWDEI YVIHCTNTS LENFIYDFD PFNEKDSQY RRSYAIVIT NSQLKRIDF FYLPFSNPS NLMRLSLTN SSIEYINDS AFEDYRFSD LVLSNNKLH SMSFVEKLP TNLNKLYLD GNHFSSVGN VFANLIHLT HLDLSNCRI QNIDPDSFS SLKSLIFIN VSNNRLVRG FFVFNFSPI PFFDLSFNK MEHLDFKGD NLHFGELDI SHNPLKNID FLNKIKFGD LKLASTNVS GDFNFLLLN NVYFSLDLN NNKLVNLHS DLFKVETYK GPFRKNINF FNSSISNID RHAFKNFGY YYDYSPVDN NPPILNLSS NAITELKNY TFEGSFIST LDLSKCLIQ NIQIKAFSG LYLTDTLNL SLNRISHLN ENVFKNLTL VQTIDLSFN FLKKLNNQL FIDCHSLKF INLSNSRIE LIKEWAFAG SDNLLELNL ENNFLEKIN FRNFSRLEL LNLHNNKLS NLDLSFLCN LSAKNVVMS HNKIGKLLN NSCATVKSP QEMDLSKSS ICSIEAKTF RGLFWLEVL TLRDNFIET ILPETFTYL KRLKQLDLS ANRIAFLSE NAFAFGNRL NMLNITFVN ANLTGHLFK WLNHVVTLN ISNSQVSLL KGCFKGLKY LENFYFHKS EVVEVIPGA FQGLDSLKQ FNVHSIFRN TKVLQSGTF EGLKYLDHL NLSHLEITV IETQAFEGL TTLLKLCLS RNNLTKIIS GTFVGLDHL KMLDLSRNK ILQLEVRAF YGLYSLREL HLNRNNLTT LSLGTFQQF PTLEVLNLR RNNINKILT GTFSNLKFL KTLDLSYNN ITKLKMETL ITLSNLKKI DLTANHLIQ MEHLDLIKS LRHLEFVGL NDNKWKCDN LVSMLIAFQ NYSVNWLST ESATFTHEN IDGIKCFDI CNFFYCVDE EDLTWRN
- >TC000097 RA
atgtttgtg gcaagcttg tgcatcctg gcatcactt atctcaact tcgagcccg tattgcgtt gaagagtgg gacgaaatc tacgtaatt cattgcacg aacacaagt cttgaaaat ttcatttac gactttgat ccgttcaac gagaaagac tcccagtat cgaagaagc tacgccatt gtaatcacc aattcacaa ttgaaacga atcgacttt ttctacctc cccttttcg aatccttca aatctgatg cgactgtct ttaaccaac tcttctatc gagtacatc aacgattca gctttcgag gattatcgt ttcagcgat ttggttttg agcaacaac aaattacac agtatgtcg tttgtcgaa aaattacca acaaattta aacaaactg tatttggac ggaaaccat ttcagctca gttggaaat gttttcgcc aatttaatt cacttgact cacctagat ttaagcaac tgtcgaata caaaacatc gatccggac agtttttcc agcttgaaa agtttgatc tttatcaat gttagtaac aacagactt gtgcgaggt tttttcgta tttaacttc tccccaatt ccgtttttc gacctgagt tttaacaaa atggaacac cttgatttc aaaggcgat aatttacat tttggcgaa ttggatatt agccacaac cctttgaaa aatatcgat tttttgaac aaaattaaa tttggcgat ttaaaacta gcaagtact aatgtaagt ggcgacttt aattttcta ttactcaac aatgtgtac ttttcgttg gatttaaac aacaataag ctggttaat ttacattcg gacttgttt aaagtggag acgtataaa ggccccttc aggaaaaac attaatttc tttaattct tcaatcagc aacattgac agacatgcg tttaaaaac ttcggttac tattatgat tactcaccc gttgataat aacccacca attctcaat cttagttca aacgcgatc accgaactc aaaaattac acttttgag ggcagtttt attagcact ttggacctc tcaaagtgt ttgattcaa aatatacaa atcaaggct ttctccggt ttatatcta accgacact ttaaactta agcctcaac cgaatatca cacttaaac gaaaacgtt ttcaaaaac ttgactctt gtccaaacg attgatttg tcgttcaat ttcctgaaa aaactcaac aatcagtta tttatcgac tgtcacagt ttgaaattt ataaactta tccaattcc aggattgag ttaataaaa gaatgggcc tttgccgga agtgataat ttactagaa ctaaacctt gaaaataat tttttggag aaaatcaat tttcgtaat ttcagtcgc ctcgaattg ctaaacctg cacaacaat aaactttca aatttagat ttgtcgttt ctgtgtaat ttatcggcg aaaaatgtg gtcatgtca cataataaa ataggaaaa ttattaaac aactcttgt gcaactgta aaatcgcca caagaaatg gatttatcc aaatcgtca atttgtagc atcgaagcg aaaacattt cgtggtttg ttttggttg gaagttctc actttgagg gacaacttt atcgaaaca attttaccc gaaactttc acgtatttg aagcgatta aaacaactc gatctttcc gccaacaga attgcgttt ttatcggag aatgcgttc gcttttgga aaccgtctc aatatgttg aatattacg ttcgttaat gcaaacttg actggtcat ttatttaag tggttgaat catgtcgtg actttgaac atttccaat tcgcaagtg agtttgttg aagggttgc ttcaaaggt ctcaaatac ttggagaat ttttatttc cataaaagt gaagttgtg gaggtaatt cctggtgca tttcagggt ctagacagt ttgaaacaa ttcaacgta cactcaatt tttcgaaac accaaagtt ttgcaaagt gggaccttt gaaggtttg aaatatttg gatcatttg aatttgtcg catcttgaa ataacggtg attgaaact caagctttt gaaggcctt acaactctt ctcaaattg tgtttgagt cgaaataat ttgaccaaa attataagt gggactttt gtgggtttg gaccatttg aaaatgtta gatttgtcg aggaataaa attttgcag ttggaagtg cgggccttt tatggcttg tattcactg cgtgaatta cacttgaat cggaataat ttaacgact ttgtctctc ggaactttt caacaattc cctacgttg gaagtgttg aatttgcgc aggaataac ataaataaa attctgact gggactttt tccaatttg aaatttcta aaaactttg gatttgtcg tataataac atcacgaaa cttaaaatg gaaacgctt attacgttg agcaatttg aaaaagatt gatttgact gcgaatcat ttgattcag atggaacat ttggatttg atcaagtct ttgagacac ttggagttt gtaggtctt aatgataac aagtggaag tgcgataat ttggtgtca atgttgatc gcgtttcaa aattatagc gtcaactgg ttgtctact gaaagtgca acttttacg catgaaaat atagacggg attaagtgt tttgatatt tgtaatttc ttttattgt gttgacgag gaagacctc acttggagg aattga
- >TC000097 RA
gagatcaaa tatcttgtt ccgtttccg gtgatacac cgaaatttt tgtgtgtca gtgaaaaat aaattaaaa tgtgtgaaa acaaattta attagtgca acgtgtaaa gtttttgta acaagtata tcgtcttgc agacagacc aaaaatgtt tgtggcaag cttgtgcat cctggcatc acttatctc aacttcgag cccgtattg cgttgaaga gtgggacga aatctacgt aattcattg cacgaacac aagtcttga aaatttcat ttacgactt tgatccgtt caacgagaa agactccca gtatcgaag aagctacgc cattgtaat caccaattc acaattgaa acgaatcga ctttttcta cctcccctt ttcgaatcc ttcaaatct gatgcgact gtctttaac caactcttc tatcgagta catcaacga ttcagcttt cgaggatta tcgtttcag cgatttggt tttgagcaa caacaaatt acacagtat gtcgtttgt cgaaaaatt accaacaaa tttaaacaa actgtattt ggacggaaa ccatttcag ctcagttgg aaatgtttt cgccaattt aattcactt gactcacct agatttaag caactgtcg aatacaaaa catcgatcc ggacagttt ttccagctt gaaaagttt gatctttat caatgttag taacaacag acttgtgcg aggtttttt cgtatttaa cttctcccc aattccgtt tttcgacct gagttttaa caaaatgga acaccttga tttcaaagg cgataattt acattttgg cgaattgga tattagcca caacccttt gaaaaatat cgatttttt gaacaaaat taaatttgg cgatttaaa actagcaag tactaatgt aagtggcga ctttaattt tctattact caacaatgt gtacttttc gttggattt aaacaacaa taagctggt taatttaca ttcggactt gtttaaagt ggagacgta taaaggccc cttcaggaa aaacattaa tttctttaa ttcttcaat cagcaacat tgacagaca tgcgtttaa aaacttcgg ttactatta tgattactc acccgttga taataaccc accaattct caatcttag ttcaaacgc gatcaccga actcaaaaa ttacacttt tgagggcag ttttattag cactttgga cctctcaaa gtgtttgat tcaaaatat acaaatcaa ggctttctc cggtttata tctaaccga cactttaaa cttaagcct caaccgaat atcacactt aaacgaaaa cgttttcaa aaacttgac tcttgtcca aacgattga tttgtcgtt caatttcct gaaaaaact caacaatca gttatttat cgactgtca cagtttgaa atttataaa cttatccaa ttccaggat tgagttaat aaaagaatg ggcctttgc cggaagtga taatttact agaactaaa ccttgaaaa taatttttt ggagaaaat caattttcg taatttcag tcgcctcga attgctaaa cctgcacaa caataaact ttcaaattt agatttgtc gtttctgtg taatttatc ggcgaaaaa tgtggtcat gtcacataa taaaatagg aaaattatt aaacaactc ttgtgcaac tgtaaaatc gccacaaga aatggattt atccaaatc gtcaatttg tagcatcga agcgaaaac atttcgtgg tttgttttg gttggaagt tctcacttt gagggacaa ctttatcga aacaatttt acccgaaac tttcacgta tttgaagcg attaaaaca actcgatct ttccgccaa cagaattgc gtttttatc ggagaatgc gttcgcttt tggaaaccg tctcaatat gttgaatat tacgttcgt taatgcaaa cttgactgg tcatttatt taagtggtt gaatcatgt cgtgacttt gaacatttc caattcgca agtgagttt gttgaaggg ttgcttcaa aggtctcaa atacttgga gaattttta tttccataa aagtgaagt tgtggaggt aattcctgg tgcatttca gggtctaga cagtttgaa acaattcaa cgtacactc aatttttcg aaacaccaa agttttgca aagtgggac ctttgaagg tttgaaata tttggatca tttgaattt gtcgcatct tgaaataac ggtgattga aactcaagc ttttgaagg ccttacaac tcttctcaa attgtgttt gagtcgaaa taatttgac caaaattat aagtgggac ttttgtggg tttggacca tttgaaaat gttagattt gtcgaggaa taaaatttt gcagttgga agtgcgggc cttttatgg cttgtattc actgcgtga attacactt gaatcggaa taatttaac gactttgtc tctcggaac ttttcaaca attccctac gttggaagt gttgaattt gcgcaggaa taacataaa taaaattct gactgggac tttttccaa tttgaaatt tctaaaaac tttggattt gtcgtataa taacatcac gaaacttaa aatggaaac gcttattac gttgagcaa tttgaaaaa gattgattt gactgcgaa tcatttgat tcagatgga acatttgga tttgatcaa gtctttgag acacttgga gtttgtagg tcttaatga taacaagtg gaagtgcga taatttggt gtcaatgtt gatcgcgtt tcaaaatta tagcgtcaa ctggttgtc tactgaaag tgcaacttt tacgcatga aaatataga cgggattaa gtgttttga tatttgtaa tttctttta ttgtgttga cgaggaaga cctcacttg gaggaattg aattatcca aggtttata ataaatatt ttagtgtta t
