Status
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Gene information
General information
TC000086 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 11487326..11503786
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000086
    Gene ontology

    GO terms for Tribolium

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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02972

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000086 RA
      MILFNGKKC IALAGLIFI LCCHRCSCK NSKNRGNKT 
      DNLFLDSES SLQTLTKCP PNIKTKCPQ DCIIKNNKP 
      TCRCFPGYT FKHKKCIDI NECKNGKHK CEKKCINLP 
      GSYKCACPQ GFRLLPDGK TCEDINECL LRNGHGPCQ 
      DLCENTHGS YKCICRNGT KLAPNGHSC TDLDECSEK 
      NSGCSHGCI NTHGRVYCT CPIGLELGP DWKTCKDVN 
      ECDDEKIKG SCPYGCVNT VGSYFCAKA MADQPVDED 
      EDYEGEEDD YETEEYDDK DYEEEQERN KTDKEYNGI 
      DNVPELNFD KVDEALEIN KNENVISNN KSEVIDKGV 
      HGKEEITNN NTDEYEDED DYDDEEEEE EDYEDETDS 
      TEKPFTIVD SLPTTTRTT EEIATTLAP IAQSEDDEE 
      SFTEETVNK PEESDLPES RTETENLTT TEDNERAVD 
      EDDEDYDED EDEDYDDED DDFGDHDRC IEGYEMNDK 
      GDCVDIDEC LSSSPPCSH DCENLEGGY KCLCPPGYE 
      LDIPDFNTC QDLNECISN PCAYKCVNE IGTFHCECP 
      EGYLLLANN SCTKITCPQ DHVVEHGVQ RCICPRGYT 
      QSRNKITCD DVNECDGDH GCTHLCINH EGSYSCSCY 
      DGFALERDN RTCSDVDEC RKGVCGQKC ENTEGSFRC 
      FCDKGYELA NDGKTCLDI DECEREDSC EQLCHNFDG 
      GYECGCEKG FKLDEDESS CYDINECES NNGNCSQIC 
      ENFDGGFVC KCHNYYKLS HDNYTCVPL DPCQENNGG 
      CSHICTNDN NEAVCKCPE GYELEDKSC VKINPCTIN 
      NGGCSHFCQ HINDQINCH CPERYDLVN DTLCVEQNP 
      CLDHNGGCS HICTTVNNQ VKCECPEGY ELDDKTCLK 
      INPCTIHNG GCSHLCTIV NNQVACECP EGYELEAKT 
      CLKINPCTI NNGGCSHFC EFVDDQINC LCPEGHDLV 
      NDTLCVERN PCLEDNGGC SHFCAFFNH TIICSCPHT 
      HELHNETSC YLKNPCLDQ NGGCSHFCH FENDQLSCS 
      CPDNFYLEG RICYRKDPC LVKNGGCSH QCFSNDLGE 
      VECSCPEGY DLRDKTCII PDPCDNNNG NCSHECVSD 
      GIKATCHCP EDYVRINET HCVPDLCLV ENGGCSHKC 
      DFDGDQVTC GCPHAYDLG PEGRICAPR DPCTVQNGG 
      CSHLCQNFG GTVKCDCPE FYNLSDDGH TCVRVSLCS 
      IDNGGCSHE CTEEGNYVY CLCPEGYSL EDKTCIPID 
      PCRKNNGGC SHECTSEAN IVYCLCPEG YELDGKDCI 
      LNPCHDNPC SHFCEVVDG LAVCKCPQN YELVGKTCT 
      RINPCQINN GNCSHTCVS NDGIATCTC PPGLKLQDK 
      THCVVENPC LIDNGGCSH QCHNQDGTA RCTCPPGYI 
      LRPNNRVCK EVNECKIRR GGCSHGCRN LPGSFQCTC 
      PPGFELGFD NRTCVDVNE CLEDNGGCH MTCINYKGG 
      HKCGCEQGY ALQEDGRHC EAITFRDCR VPVAPDQGI 
      IKCIGGNYE KGSLAPVGT KCMVWCNEG HKLVGESVR 
      VCQSTGSWG GKEPQCVAA ACPRPGPIQ NGWLLPSNC 
      NNGFTYGGE KCRIYCKRG YKLRPRFEF LSCSEDLIW 
      QPLEAVNRL HDVCVRDIE HTYIKCPGG GHLTYTLPQ 
      GQRHMYAKI PQPETNANW IRDVKAEPS WGMQLGTIL 
      PAGHTQLTF YVDPEDPSL LLHSNLTSC KLVITILDI 
      EDPKVTGCP TNMEYTLPR GHSSQLVFW KEPQFSDNV 
      GIARLYKSR EPGTSFGVG LHHVNYVAS DEAGNRAFC 
      HFTVDIKEE IDSPSNDLA SNLEKRSEK YRAVLVCPS 
      GVQYISKPP DLYNNKIAT EAGCYWRHV RVSRPLQPQ 
      GLRIPPHRI HNESRLPPH LQKKHRYFE WYDKCC
    CDS sequence
    • >TC000086 RA
      atgattctg ttcaacgga aaaaagtgc atcgccttg 
      gctggatta atatttatt ctttgttgc caccgctgc 
      agctgtaaa aactccaaa aatcgggga aataaaacg 
      gataattta tttttagat agtgaatcg tctctacaa 
      actttaaca aaatgccct ccaaacatc aaaaccaaa 
      tgtccacaa gactgcatc atcaaaaac aacaaaccg 
      acgtgtcgc tgcttccca ggctacacc ttcaaacat 
      aaaaaatgc atcgacata aatgagtgc aaaaatggt 
      aaacataaa tgcgagaaa aaatgcatc aaccttccg 
      ggttcctac aaatgcgcc tgccctcag ggcttccgc 
      ctcctccca gatggtaaa acctgcgaa gacattaac 
      gaatgcctc ctgcgaaat gggcatggg ccgtgtcag 
      gacttgtgc gaaaacacc catggctcg tacaagtgc 
      atttgtcgc aatgggacc aaattggcg cccaatggg 
      cactcgtgc accgatttg gacgaatgc tcggagaag 
      aattcaggc tgttcgcat gggtgtatc aacacccat 
      ggacgtgtg tactgcact tgtcccatt gggctggag 
      ctgggcccg gactggaag acttgcaaa gatgttaac 
      gagtgtgac gatgagaag attaagggt agctgccca 
      tatgggtgt gttaacaca gttgggtcg tatttttgt 
      gccaaagcc atggcggat caaccggtt gatgaagac 
      gaggattat gaaggtgaa gaagacgat tacgaaact 
      gaagaatac gatgataaa gactatgaa gaagagcaa 
      gagaggaac aaaactgat aaggaatac aacggtatt 
      gataacgtg ccagaactg aactttgat aaggtcgat 
      gaggctctt gaaataaat aaaaatgaa aatgtgata 
      agcaataat aagtctgaa gttattgat aagggtgtg 
      catggaaag gaagaaatc acgaataac aacaccgac 
      gagtatgaa gacgaagac gattacgac gatgaagaa 
      gaagaagaa gaggattac gaagatgaa acagactcg 
      actgaaaaa ccgttcaca attgttgat tctttacca 
      accaccacg agaactact gaagaaatc gccacaacg 
      ttggctccg attgcgcaa agtgaagac gatgaagaa 
      agttttact gaagaaact gtgaacaaa ccggaagaa 
      agcgatttg cctgaaagc agaactgaa acggagaat 
      ttaacaacg actgaagat aacgaaaga gcggttgat 
      gaggatgac gaagattat gacgaagat gaggatgaa 
      gattatgac gatgaagac gacgatttt ggggatcat 
      gatcgctgt attgaaggc tacgaaatg aatgataag 
      ggcgattgt gtcgatatc gacgaatgc ctctccagc 
      tcacccccc tgttcgcac gactgcgag aacctcgaa 
      ggcggctac aaatgcctc tgccccccc ggctacgag 
      ctcgacatc cccgacttc aacacctgc caagacctc 
      aacgagtgc atctccaac ccttgtgcc tacaaatgc 
      gtcaacgaa atcggcacc ttccactgc gaatgcccc 
      gaaggctac ctcctcctg gccaacaac agctgcacc 
      aaaatcacc tgcccccaa gaccacgtc gtcgaacac 
      ggcgtccag cgctgcatc tgcccccgt ggctacacc 
      cagagtcga aacaagatc acttgcgac gatgttaac 
      gaatgtgac ggcgaccac ggctgcacc catctgtgc 
      atcaaccac gagggctcc tactcatgc tcgtgctac 
      gatgggttc gcccttgaa cgcgataat aggacttgc 
      agcgatgtt gatgagtgt cggaagggc gtttgtggg 
      caaaaatgc gaaaacacg gaagggagt tttcggtgt 
      ttttgtgac aaagggtac gaacttgca aatgatggg 
      aaaacttgt cttgatatt gatgaatgt gaacgggag 
      gattcttgc gaacagttg tgccacaat tttgacggg 
      gggtacgaa tgtgggtgt gaaaaaggg ttcaagctg 
      gatgaggat gagtcgagt tgttacgac atcaacgag 
      tgtgagtca aacaatggg aactgctcc caaatttgt 
      gaaaacttc gacggtggt tttgtctgc aagtgtcac 
      aattactac aagttgtcc cacgacaat tacacctgt 
      gtcccactt gatccctgt caagagaac aacgggggg 
      tgttcccac atttgcaca aatgacaat aacgaagcg 
      gtttgtaaa tgtcccgaa ggatacgaa ctggaggac 
      aaaagttgc gttaaaatc aacccttgt accatcaac 
      aacgggggt tgttcccac ttttgtcaa catatcaac 
      gaccaaatc aattgtcat tgtcccgaa agatacgat 
      ttggtcaac gacacactt tgtgttgag caaaatccc 
      tgtctggac cacaacggg ggttgttcc cacatttgc 
      acaaccgtc aataaccaa gtaaagtgt gaatgtccc 
      gaaggttac gaactggat gacaaaacc tgtctcaaa 
      atcaaccct tgtacaata cacaacggg ggttgttcc 
      cacctttgc acaatcgtc aataaccaa gtggcttgt 
      gaatgtccc gaaggttac gaactggag gccaaaacc 
      tgtctcaaa atcaaccct tgtacaatc aacaacggg 
      ggttgttcc cacttttgt gaattcgtt gacgaccaa 
      atcaattgt ctatgcccc gaaggacat gacttggtc 
      aatgacaca ctttgtgtt gagcgaaat ccctgtctg 
      gaggacaac gggggttgt tcccacttt tgcgccttc 
      ttcaaccac actatcatt tgttcctgt ccccacact 
      cacgagtta cataacgaa acgtcctgc tacctcaaa 
      aacccctgt ctggaccaa aatgggggt tgttcccac 
      ttttgtcat ttcgaaaac gaccaattg tcctgctcc 
      tgccctgac aatttctac cttgagggc aggatttgt 
      taccgtaag gacccatgt cttgtcaaa aacggcggg 
      tgttcgcac caatgcttt tcaaacgat ttgggcgaa 
      gttgagtgt tcgtgtccc gaaggctac gatttgagg 
      gacaagacg tgcataatc cctgatccc tgtgacaat 
      aacaacgga aactgctca cacgagtgt gtctctgac 
      gggattaaa gccacgtgt cattgtccc gaggattat 
      gtgcgaatc aatgagacg cattgtgtt cccgatttg 
      tgtttggtt gaaaatggg ggttgttcc cataagtgt 
      gattttgac ggggatcaa gtgacttgt gggtgcccc 
      catgcgtat gatttgggg cccgagggg cgcatttgc 
      gctccgagg gacccttgc accgtccaa aatggaggt 
      tgttcccac ttatgccaa aattttgga ggcacagtc 
      aagtgtgac tgtcctgag ttttataat ttgtcagac 
      gatggtcac acttgtgtt agggttagt ttatgttca 
      attgacaat ggggggtgt tcccatgaa tgtaccgaa 
      gagggaaac tatgtttac tgtctttgt cctgaggga 
      tattcgctt gaagataaa acttgcatc ccaattgat 
      ccctgtcgt aaaaacaac ggtggttgc tcacatgaa 
      tgtacctct gaagcaaac attgtctat tgtttgtgt 
      cccgagggt tacgaactc gatggcaag gattgcatc 
      cttaatccc tgtcatgat aatccctgt tcccacttt 
      tgcgaagtt gtggacggg ttagcggtt tgcaaatgc 
      ccacaaaac tacgaattg gttggtaaa acctgcaca 
      aggattaat ccctgtcag attaataac gggaattgt 
      tcacacacg tgtgtgtca aatgacggg attgcaaca 
      tgcacgtgt ccgccagga ctaaaacta caagacaaa 
      acacactgt gttgtcgaa aatccttgt ttgattgac 
      aacgggggt tgttcccac caatgtcac aatcaagat 
      ggcacagct cgctgcact tgtcccccg ggttacatc 
      ctccgaccg aacaaccgc gtctgtaag gaggtcaat 
      gagtgcaaa atccggcga ggtggatgt tcacacggt 
      tgtcgaaac ctgcctggg agtttccaa tgcacttgt 
      ccgcccggg tttgagtta gggtttgat aaccggact 
      tgtgtcgat gtgaacgag tgtttggag gacaatggg 
      ggctgtcat atgacttgt attaactac aaagggggg 
      cacaagtgt gggtgtgag cagggctac gccttgcag 
      gaggatggg aggcactgt gaagctatt acctttcgg 
      gattgcaga gttcctgtt gcgcccgat caagggatt 
      attaagtgt attggaggg aattacgaa aaagggagt 
      ttggcacca gtggggaca aaatgcatg gtttggtgc 
      aacgaggga cacaagcta gtgggggag tcggtgagg 
      gtgtgtcag agtacgggg agttggggc ggcaaggag 
      ccacagtgt gtagccgct gcttgcccg cgccctggc 
      cctatccaa aacgggtgg ctcttgccc agcaactgc 
      aataacggt tttacttac gggggtgaa aaatgccga 
      atttattgc aaaaggggc tacaaatta cgcccccga 
      tttgagttt ctctcatgc agtgaagac ttgatttgg 
      caacccctg gaagcggtg aatcgactt catgacgtt 
      tgtgttcga gacattgaa catacgtat attaagtgc 
      cccggaggg ggtcacctg acctatact ctgcctcag 
      ggacagagg cacatgtat gcaaagata ccccagcct 
      gagaccaac gccaactgg atcagagat gtcaaggct 
      gagcccagt tggggcatg cagttgggc acaattcta 
      ccagctggt cacacccag ttgacgttt tacgtcgac 
      cccgaagac ccaagtctt ctcctccac tctaattta 
      acttcatgt aaactggtt attacaatt ttggacata 
      gaagaccct aaagtgacc ggatgcccc accaacatg 
      gaatacacc cttccaagg gggcactca agtcaactg 
      gttttctgg aaagaaccc caatttagt gataacgtc 
      gggatcgca agactttac aagtcgaga gaacctggg 
      acttcgttc ggtgttggc ctacatcac gtcaattac 
      gtggcaagt gacgaagcc ggaaatcgt gctttttgc 
      cattttact gtcgacata aaagaagaa atcgattca 
      ccctcaaat gacctagct tcaaatttg gaaaaaagg 
      tcggaaaaa tatcgcgcc gttttggtt tgcccgagt 
      ggagtgcaa tacatatcg aaacctccc gatttatac 
      aataacaag atagcaact gaagctggg tgttactgg 
      agacacgtg cgtgtgtca cgcccattg cagccccaa 
      gggttgaga atcccaccc catagaatt cacaacgag 
      agtcgtctt ccaccccac ttgcagaag aagcaccgg 
      tacttcgaa tggtacgat aagtgttgc taa
    mRNA sequence
    • >TC000086 RA
      tgggatgca aagaaggtg caacttatt ggcgcctgt 
      acaaatggt ccaacataa gagagaaaa atagtgcag 
      aatgattct gttcaacgg aaaaaagtg catcgcctt 
      ggctggatt aatatttat tctttgttg ccaccgctg 
      cagctgtaa aaactccaa aaatcgggg aaataaaac 
      ggataattt atttttaga tagtgaatc gtctctaca 
      aactttaac aaaatgccc tccaaacat caaaaccaa 
      atgtccaca agactgcat catcaaaaa caacaaacc 
      gacgtgtcg ctgcttccc aggctacac cttcaaaca 
      taaaaaatg catcgacat aaatgagtg caaaaatgg 
      taaacataa atgcgagaa aaaatgcat caaccttcc 
      gggttccta caaatgcgc ctgccctca gggcttccg 
      cctcctccc agatggtaa aacctgcga agacattaa 
      cgaatgcct cctgcgaaa tgggcatgg gccgtgtca 
      ggacttgtg cgaaaacac ccatggctc gtacaagtg 
      catttgtcg caatgggac caaattggc gcccaatgg 
      gcactcgtg caccgattt ggacgaatg ctcggagaa 
      gaattcagg ctgttcgca tgggtgtat caacaccca 
      tggacgtgt gtactgcac ttgtcccat tgggctgga 
      gctgggccc ggactggaa gacttgcaa agatgttaa 
      cgagtgtga cgatgagaa gattaaggg tagctgccc 
      atatgggtg tgttaacac agttgggtc gtatttttg 
      tgccaaagc catggcgga tcaaccggt tgatgaaga 
      cgaggatta tgaaggtga agaagacga ttacgaaac 
      tgaagaata cgatgataa agactatga agaagagca 
      agagaggaa caaaactga taaggaata caacggtat 
      tgataacgt gccagaact gaactttga taaggtcga 
      tgaggctct tgaaataaa taaaaatga aaatgtgat 
      aagcaataa taagtctga agttattga taagggtgt 
      gcatggaaa ggaagaaat cacgaataa caacaccga 
      cgagtatga agacgaaga cgattacga cgatgaaga 
      agaagaaga agaggatta cgaagatga aacagactc 
      gactgaaaa accgttcac aattgttga ttctttacc 
      aaccaccac gagaactac tgaagaaat cgccacaac 
      gttggctcc gattgcgca aagtgaaga cgatgaaga 
      aagttttac tgaagaaac tgtgaacaa accggaaga 
      aagcgattt gcctgaaag cagaactga aacggagaa 
      tttaacaac gactgaaga taacgaaag agcggttga 
      tgaggatga cgaagatta tgacgaaga tgaggatga 
      agattatga cgatgaaga cgacgattt tggggatca 
      tgatcgctg tattgaagg ctacgaaat gaatgataa 
      gggcgattg tgtcgatat cgacgaatg cctctccag 
      ctcaccccc ctgttcgca cgactgcga gaacctcga 
      aggcggcta caaatgcct ctgcccccc cggctacga 
      gctcgacat ccccgactt caacacctg ccaagacct 
      caacgagtg catctccaa cccttgtgc ctacaaatg 
      cgtcaacga aatcggcac cttccactg cgaatgccc 
      cgaaggcta cctcctcct ggccaacaa cagctgcac 
      caaaatcac ctgccccca agaccacgt cgtcgaaca 
      cggcgtcca gcgctgcat ctgcccccg tggctacac 
      ccagagtcg aaacaagat cacttgcga cgatgttaa 
      cgaatgtga cggcgacca cggctgcac ccatctgtg 
      catcaacca cgagggctc ctactcatg ctcgtgcta 
      cgatgggtt cgcccttga acgcgataa taggacttg 
      cagcgatgt tgatgagtg tcggaaggg cgtttgtgg 
      gcaaaaatg cgaaaacac ggaagggag ttttcggtg 
      tttttgtga caaagggta cgaacttgc aaatgatgg 
      gaaaacttg tcttgatat tgatgaatg tgaacggga 
      ggattcttg cgaacagtt gtgccacaa ttttgacgg 
      ggggtacga atgtgggtg tgaaaaagg gttcaagct 
      ggatgagga tgagtcgag ttgttacga catcaacga 
      gtgtgagtc aaacaatgg gaactgctc ccaaatttg 
      tgaaaactt cgacggtgg ttttgtctg caagtgtca 
      caattacta caagttgtc ccacgacaa ttacacctg 
      tgtcccact tgatccctg tcaagagaa caacggggg 
      gtgttccca catttgcac aaatgacaa taacgaagc 
      ggtttgtaa atgtcccga aggatacga actggagga 
      caaaagttg cgttaaaat caacccttg taccatcaa 
      caacggggg ttgttccca cttttgtca acatatcaa 
      cgaccaaat caattgtca ttgtcccga aagatacga 
      tttggtcaa cgacacact ttgtgttga gcaaaatcc 
      ctgtctgga ccacaacgg gggttgttc ccacatttg 
      cacaaccgt caataacca agtaaagtg tgaatgtcc 
      cgaaggtta cgaactgga tgacaaaac ctgtctcaa 
      aatcaaccc ttgtacaat acacaacgg gggttgttc 
      ccacctttg cacaatcgt caataacca agtggcttg 
      tgaatgtcc cgaaggtta cgaactgga ggccaaaac 
      ctgtctcaa aatcaaccc ttgtacaat caacaacgg 
      gggttgttc ccacttttg tgaattcgt tgacgacca 
      aatcaattg tctatgccc cgaaggaca tgacttggt 
      caatgacac actttgtgt tgagcgaaa tccctgtct 
      ggaggacaa cgggggttg ttcccactt ttgcgcctt 
      cttcaacca cactatcat ttgttcctg tccccacac 
      tcacgagtt acataacga aacgtcctg ctacctcaa 
      aaacccctg tctggacca aaatggggg ttgttccca 
      cttttgtca tttcgaaaa cgaccaatt gtcctgctc 
      ctgccctga caatttcta ccttgaggg caggatttg 
      ttaccgtaa ggacccatg tcttgtcaa aaacggcgg 
      gtgttcgca ccaatgctt ttcaaacga tttgggcga 
      agttgagtg ttcgtgtcc cgaaggcta cgatttgag 
      ggacaagac gtgcataat ccctgatcc ctgtgacaa 
      taacaacgg aaactgctc acacgagtg tgtctctga 
      cgggattaa agccacgtg tcattgtcc cgaggatta 
      tgtgcgaat caatgagac gcattgtgt tcccgattt 
      gtgtttggt tgaaaatgg gggttgttc ccataagtg 
      tgattttga cggggatca agtgacttg tgggtgccc 
      ccatgcgta tgatttggg gcccgaggg gcgcatttg 
      cgctccgag ggacccttg caccgtcca aaatggagg 
      ttgttccca cttatgcca aaattttgg aggcacagt 
      caagtgtga ctgtcctga gttttataa tttgtcaga 
      cgatggtca cacttgtgt tagggttag tttatgttc 
      aattgacaa tggggggtg ttcccatga atgtaccga 
      agagggaaa ctatgttta ctgtctttg tcctgaggg 
      atattcgct tgaagataa aacttgcat cccaattga 
      tccctgtcg taaaaacaa cggtggttg ctcacatga 
      atgtacctc tgaagcaaa cattgtcta ttgtttgtg 
      tcccgaggg ttacgaact cgatggcaa ggattgcat 
      ccttaatcc ctgtcatga taatccctg ttcccactt 
      ttgcgaagt tgtggacgg gttagcggt ttgcaaatg 
      cccacaaaa ctacgaatt ggttggtaa aacctgcac 
      aaggattaa tccctgtca gattaataa cgggaattg 
      ttcacacac gtgtgtgtc aaatgacgg gattgcaac 
      atgcacgtg tccgccagg actaaaact acaagacaa 
      aacacactg tgttgtcga aaatccttg tttgattga 
      caacggggg ttgttccca ccaatgtca caatcaaga 
      tggcacagc tcgctgcac ttgtccccc gggttacat 
      cctccgacc gaacaaccg cgtctgtaa ggaggtcaa 
      tgagtgcaa aatccggcg aggtggatg ttcacacgg 
      ttgtcgaaa cctgcctgg gagtttcca atgcacttg 
      tccgcccgg gtttgagtt agggtttga taaccggac 
      ttgtgtcga tgtgaacga gtgtttgga ggacaatgg 
      gggctgtca tatgacttg tattaacta caaaggggg 
      gcacaagtg tgggtgtga gcagggcta cgccttgca 
      ggaggatgg gaggcactg tgaagctat tacctttcg 
      ggattgcag agttcctgt tgcgcccga tcaagggat 
      tattaagtg tattggagg gaattacga aaaagggag 
      tttggcacc agtggggac aaaatgcat ggtttggtg 
      caacgaggg acacaagct agtggggga gtcggtgag 
      ggtgtgtca gagtacggg gagttgggg cggcaagga 
      gccacagtg tgtagccgc tgcttgccc gcgccctgg 
      ccctatcca aaacgggtg gctcttgcc cagcaactg 
      caataacgg ttttactta cgggggtga aaaatgccg 
      aatttattg caaaagggg ctacaaatt acgcccccg 
      atttgagtt tctctcatg cagtgaaga cttgatttg 
      gcaacccct ggaagcggt gaatcgact tcatgacgt 
      ttgtgttcg agacattga acatacgta tattaagtg 
      ccccggagg gggtcacct gacctatac tctgcctca 
      gggacagag gcacatgta tgcaaagat accccagcc 
      tgagaccaa cgccaactg gatcagaga tgtcaaggc 
      tgagcccag ttggggcat gcagttggg cacaattct 
      accagctgg tcacaccca gttgacgtt ttacgtcga 
      ccccgaaga cccaagtct tctcctcca ctctaattt 
      aacttcatg taaactggt tattacaat tttggacat 
      agaagaccc taaagtgac cggatgccc caccaacat 
      ggaatacac ccttccaag ggggcactc aagtcaact 
      ggttttctg gaaagaacc ccaatttag tgataacgt 
      cgggatcgc aagacttta caagtcgag agaacctgg 
      gacttcgtt cggtgttgg cctacatca cgtcaatta 
      cgtggcaag tgacgaagc cggaaatcg tgctttttg 
      ccattttac tgtcgacat aaaagaaga aatcgattc 
      accctcaaa tgacctagc ttcaaattt ggaaaaaag 
      gtcggaaaa atatcgcgc cgttttggt ttgcccgag 
      tggagtgca atacatatc gaaacctcc cgatttata 
      caataacaa gatagcaac tgaagctgg gtgttactg 
      gagacacgt gcgtgtgtc acgcccatt gcagcccca 
      agggttgag aatcccacc ccatagaat tcacaacga 
      gagtcgtct tccacccca cttgcagaa gaagcaccg 
      gtacttcga atggtacga taagtgttg ctaacaatc 
      gccaaaaaa tattatttt cgtgtacaa gactaacaa 
      aattatttt gaataaaaa tttccaaaa tca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY