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Gene information
General information
TC000068 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 11096467..11099466
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000068
    iBeetle screen iB_02970

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000068 RA
      MATDAVGFS IVTEPKRKK SYYEDEEII IKPSTELIP 
      DEYKHEVEE NVLLHIFKT DYDDETAEE ILESAKYIN 
      YKVDSNNLH NLNTRNEFY EDIEKIKKE SVNKSGLFM 
      IFIGFFERR HPNGTIALM NERNDFIQI KDIWNMFSA 
      SNCPELKNK PKIFMFCVS GAPYAVQSD SLRHRTMAF 
      EKVYDFPAE SDMLIVYKK VDDVSPHMQ KFLATFSLN 
      IRNHSKKYD IIDLVSHIN EENSKYVPL IISTLTRKF 
      YLTPNIERE HFLSVAENS SSVQNDLKK IKEDLEKLH 
      HELQKKKKM FGFSFKKKS EEKPKIEIK RDNKVEQRK 
      SAKTGEVQR GSVGRSSAP PRPPVRSRN PSESDAKIE 
      KKKPIWKN
    CDS sequence
    • >TC000068 RA
      atggcaaca gacgcagtg ggttttagt attgtaaca 
      gagcctaaa cggaaaaaa tcttactac gaggatgaa 
      gagataata ataaaacct tcaactgaa ttaattcca 
      gacgaatac aaacacgaa gtcgaagaa aatgtgttg 
      ttgcacatt ttcaaaaca gactacgat gacgaaacg 
      gctgaagaa atactagaa tcagcaaaa tatatcaac 
      tataaagtg gacagtaac aatttacat aatcttaat 
      acacggaac gaattttac gaagatatc gagaaaatt 
      aaaaaagaa tcagtgaat aaaagtggt ctttttatg 
      atttttatt ggatttttt gaacggaga cacccgaat 
      ggtaccata gctttgatg aatgaacga aacgatttc 
      attcaaatc aaagacatt tggaacatg ttttcggca 
      agtaattgc cccgagttg aagaataaa cccaaaatt 
      ttcatgttt tgtgtctca ggggcccca tatgccgta 
      caatctgac tctttgaga caccgaact atggctttt 
      gaaaaagtt tacgacttt cctgccgaa tctgacatg 
      ttgatagtt tacaaaaaa gtcgatgat gtttcgcca 
      cacatgcaa aaatttctc gccactttt agcctcaac 
      ataagaaat cacagcaag aaatacgac atcatcgac 
      ttggtgagt cacatcaac gaagagaat agcaaatat 
      gttcctttg attatttcc acactgact cgaaaattt 
      tatttgacg ccgaatatc gaacgtgag cattttttg 
      tcagtggct gaaaacagc agtagcgtg caaaatgat 
      ctgaaaaaa attaaggaa gatttggag aaactgcac 
      cacgagctt cagaaaaag aagaaaatg tttggcttc 
      agttttaag aagaagagt gaagagaag ccgaaaatt 
      gagataaaa cgtgataat aaagtcgaa caacgtaaa 
      agtgcgaaa acaggggaa gtacagaga gggtctgtt 
      gggaggtca agtgctccg cctaggcca ccagtacga 
      tcaagaaat ccctctgaa tcggatgcg aagattgag 
      aagaagaaa ccgatttgg aagaattag
    mRNA sequence
    • >TC000068 RA
      tggtgcgtt ttgtgccta aactacgaa aaaactaat 
      catgttaga aaaatggca acagacgca gtgggtttt 
      agtattgta acagagcct aaacggaaa aaatcttac 
      tacgaggat gaagagata ataataaaa ccttcaact 
      gaattaatt ccagacgaa tacaaacac gaagtcgaa 
      gaaaatgtg ttgttgcac attttcaaa acagactac 
      gatgacgaa acggctgaa gaaatacta gaatcagca 
      aaatatatc aactataaa gtggacagt aacaattta 
      cataatctt aatacacgg aacgaattt tacgaagat 
      atcgagaaa attaaaaaa gaatcagtg aataaaagt 
      ggtcttttt atgattttt attggattt tttgaacgg 
      agacacccg aatggtacc atagctttg atgaatgaa 
      cgaaacgat ttcattcaa atcaaagac atttggaac 
      atgttttcg gcaagtaat tgccccgag ttgaagaat 
      aaacccaaa attttcatg ttttgtgtc tcaggggcc 
      ccatatgcc gtacaatct gactctttg agacaccga 
      actatggct tttgaaaaa gtttacgac tttcctgcc 
      gaatctgac atgttgata gtttacaaa aaagtcgat 
      gatgtttcg ccacacatg caaaaattt ctcgccact 
      tttagcctc aacataaga aatcacagc aagaaatac 
      gacatcatc gacttggtg agtcacatc aacgaagag 
      aatagcaaa tatgttcct ttgattatt tccacactg 
      actcgaaaa ttttatttg acgccgaat atcgaacgt 
      gagcatttt ttgtcagtg gctgaaaac agcagtagc 
      gtgcaaaat gatctgaaa aaaattaag gaagatttg 
      gagaaactg caccacgag cttcagaaa aagaagaaa 
      atgtttggc ttcagtttt aagaagaag agtgaagag 
      aagccgaaa attgagata aaacgtgat aataaagtc 
      gaacaacgt aaaagtgcg aaaacaggg gaagtacag 
      agagggtct gttgggagg tcaagtgct ccgcctagg 
      ccaccagta cgatcaaga aatccctct gaatcggat 
      gcgaagatt gagaagaag aaaccgatt tggaagaat 
      tagaggctt atttgtgat tcttctatg tagattatt 
      tgttatctt agttaaatt aataaataa aagttatat 
      taataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY