Status
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Gene information
General information
TC000045 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10719667..10729313
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000045
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02968

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000045 RA
      MDPFEAHNI TFLDLSGVD EQERQEVLD SLYAENANV 
      QLPWRVSEH KVVQKTDVK NRFVATAAA HHHQDKAAV 
      VASHPQVYL TNQVDYNAY MPPPDPSLY HHHPYSQYV 
      VYSTYLPPV VTTTIPITY TQTAPNANY ITDDEYNHQ 
      AYVPPPVAA PEEKKEEPD ANCNITTNG ETTPGRANK 
      SWASLFSNS KNSGVINGN GVNEKSSSN EAVNKQNVD 
      NNNDEDPIR NPRKAKFID PNCYRMGEF LLNYTIDGR 
      TISLQPRGL INKSNYCYI NSILQALLA CPPVYNLLV 
      GLSEQNTTA QKEKATPII DGMCRFVKE FQHLPAGLR 
      VNRRNDKNQ KKEQGLMID YDLPFEPSW IYKMLNGVR 
      SDLFLVEGR QEDAEEFLG CLLNGLNDE MLELMKLVK 
      SDLTINKFE SKTSDQDTD KEWQVMGPK NKGSITRQM 
      EFVKTPISN IFGGFLKSR IHRTGDHST YNIQPFFTL 
      QLNIEKVKT VREALEALV TKNQLEGVT NEKTNEEVE 
      AWQQVTLEE LPVILILHL KCFDFKLDG CTKIVKALE 
      FPIDLKIDS KLLSSKPMS PKEKQYKLF AVVYHDGKE 
      ATKGHYITD AFHVGYSSW IRYDDASVK SVQEDYVLK 
      PQGTRVPYL LFYRRSDTI RSK
    CDS sequence
    • >TC000045 RA
      atggatcca ttcgaggca cacaacatt acatttctg 
      gatctcagc ggggttgat gagcaagaa cgacaggag 
      gttttggat tctctgtat gcagagaat gccaatgtg 
      cagctgcct tggagggtt tcggaacat aaagtcgtc 
      caaaagacc gatgtaaaa aatcggttt gtcgccacc 
      gccgccgcc caccaccac caggacaaa gcggccgtt 
      gtggcttcc cacccccag gtgtatctc accaatcaa 
      gtcgactat aatgcctac atgccgccc cctgaccca 
      tcactatat catcatcat ccatactcg caatacgtg 
      gtctactcc acctatctg cccccagtc gtcaccacc 
      accatcccc ataacatac acgcaaacc gcccccaat 
      gctaactac atcaccgac gatgagtac aaccaccag 
      gcgtatgtg ccgccaccg gtcgccgct cccgaagaa 
      aaaaaagag gagcccgac gctaattgt aatattaca 
      acaaatgga gaaacaact ccaggaaga gcgaataaa 
      tcgtgggct agtttgttc agtaattcg aaaaactcg 
      ggcgttatt aacgggaat ggagtcaat gagaagtct 
      tcatcgaac gaggctgtg aataaacaa aacgttgat 
      aataacaat gatgaagat ccgatcagg aatcccaga 
      aaggccaag tttatcgat ccgaactgc tacagaatg 
      ggcgaattt ctgttgaat tataccatc gatggcaga 
      accataagt ttacagcca cgtggtttg atcaataag 
      agcaactat tgctacatc aattcgatt ttacaagca 
      cttttagcc tgtcctcct gtttacaat ttattagta 
      ggcttatcg gaacaaaac actacggct cagaaggag 
      aaagccacg cccatcatt gacggaatg tgccgtttc 
      gtcaaggaa ttccaacat ttgccagcc ggtctgcgt 
      gtgaatcgg cgaaacgac aaaaaccag aaaaaggaa 
      caaggttta atgatcgat tacgacctg ccatttgaa 
      ccttcttgg atttacaaa atgttgaac ggtgttaga 
      agcgatttg ttcttggtg gagggccgt caggaagac 
      gctgaagag tttctgggt tgtttatta aacggactg 
      aacgacgaa atgcttgaa ctaatgaaa ttggtgaaa 
      agcgatctt acgatcaat aaattcgag tcgaaaaca 
      agcgatcaa gacacagac aaagagtgg caggttatg 
      ggaccaaaa aacaagggt agcataacg cgacaaatg 
      gaattcgtc aagacacca attagtaat attttcggt 
      ggcttcctc aagtcgagg attcacagg actggggat 
      cattccact tacaacata caaccgttt tttaccctg 
      caattaaat atcgagaaa gtcaaaact gtgcgtgag 
      gcactcgag gctcttgtt acgaaaaat cagctggag 
      ggcgtcacc aatgagaag accaacgag gaggtggag 
      gcatggcaa caagtcact ttggaagaa cttccagtt 
      attttaatt ctgcattta aagtgcttt gattttaag 
      ctagatgga tgtacaaaa attgtcaaa gctttggag 
      tttcctatc gatttgaag attgattca aaactgtta 
      tcttccaag ccaatgtcg ccaaaagag aaacagtat 
      aagttattt gccgtcgtg tatcatgat gggaaagag 
      gcaacaaag ggtcattac attacggat gcctttcat 
      gtcggctac agcagttgg atacgatat gatgacgca 
      tcggtcaag tcagttcaa gaagactac gttttgaag 
      ccacaagga actagagtt ccctatttg ttattttac 
      cgtcgtagt gataccatt agaagcaaa taa
    mRNA sequence
    • >TC000045 RA
      acagaatgg aacgtgaaa acttaaccc cgtgtgttt 
      aatactttt gttattgct cctaaaatg ttaactttc 
      gagtagttg cagtgaaat gaatttatc acaaatatg 
      aaataacag ataccgcaa tcatggatc cattcgagg 
      cacacaaca ttacatttc tggatctca gcggggttg 
      atgagcaag aacgacagg aggttttgg attctctgt 
      atgcagaga atgccaatg tgcagctgc cttggaggg 
      tttcggaac ataaagtcg tccaaaaga ccgatgtaa 
      aaaatcggt ttgtcgcca ccgccgccg cccaccacc 
      accaggaca aagcggccg ttgtggctt cccaccccc 
      aggtgtatc tcaccaatc aagtcgact ataatgcct 
      acatgccgc cccctgacc catcactat atcatcatc 
      atccatact cgcaatacg tggtctact ccacctatc 
      tgcccccag tcgtcacca ccaccatcc ccataacat 
      acacgcaaa ccgccccca atgctaact acatcaccg 
      acgatgagt acaaccacc aggcgtatg tgccgccac 
      cggtcgccg ctcccgaag aaaaaaaag aggagcccg 
      acgctaatt gtaatatta caacaaatg gagaaacaa 
      ctccaggaa gagcgaata aatcgtggg ctagtttgt 
      tcagtaatt cgaaaaact cgggcgtta ttaacggga 
      atggagtca atgagaagt cttcatcga acgaggctg 
      tgaataaac aaaacgttg ataataaca atgatgaag 
      atccgatca ggaatccca gaaaggcca agtttatcg 
      atccgaact gctacagaa tgggcgaat ttctgttga 
      attatacca tcgatggca gaaccataa gtttacagc 
      cacgtggtt tgatcaata agagcaact attgctaca 
      tcaattcga ttttacaag cacttttag cctgtcctc 
      ctgtttaca atttattag taggcttat cggaacaaa 
      acactacgg ctcagaagg agaaagcca cgcccatca 
      ttgacggaa tgtgccgtt tcgtcaagg aattccaac 
      atttgccag ccggtctgc gtgtgaatc ggcgaaacg 
      acaaaaacc agaaaaagg aacaaggtt taatgatcg 
      attacgacc tgccatttg aaccttctt ggatttaca 
      aaatgttga acggtgtta gaagcgatt tgttcttgg 
      tggagggcc gtcaggaag acgctgaag agtttctgg 
      gttgtttat taaacggac tgaacgacg aaatgcttg 
      aactaatga aattggtga aaagcgatc ttacgatca 
      ataaattcg agtcgaaaa caagcgatc aagacacag 
      acaaagagt ggcaggtta tgggaccaa aaaacaagg 
      gtagcataa cgcgacaaa tggaattcg tcaagacac 
      caattagta atattttcg gtggcttcc tcaagtcga 
      ggattcaca ggactgggg atcattcca cttacaaca 
      tacaaccgt tttttaccc tgcaattaa atatcgaga 
      aagtcaaaa ctgtgcgtg aggcactcg aggctcttg 
      ttacgaaaa atcagctgg agggcgtca ccaatgaga 
      agaccaacg aggaggtgg aggcatggc aacaagtca 
      ctttggaag aacttccag ttattttaa ttctgcatt 
      taaagtgct ttgatttta agctagatg gatgtacaa 
      aaattgtca aagctttgg agtttccta tcgatttga 
      agattgatt caaaactgt tatcttcca agccaatgt 
      cgccaaaag agaaacagt ataagttat ttgccgtcg 
      tgtatcatg atgggaaag aggcaacaa agggtcatt 
      acattacgg atgcctttc atgtcggct acagcagtt 
      ggatacgat atgatgacg catcggtca agtcagttc 
      aagaagact acgttttga agccacaag gaactagag 
      ttccctatt tgttatttt accgtcgta gtgatacca 
      ttagaagca aataaatat acatttggc ctattaata 
      attgttaat aagtaaact tattttaat aaattaact 
      ttatacaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY