Status
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Gene information
General information
TC000027 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 15880825..15891830
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000027
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02964

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000027 RA
      MYRTDKQFM TNPATAPSR IYIGGIPKT VVAADLEEK 
      FKVHGKILG LVLQMGFAF IQFENESQS HAAIQNENN 
      TMLHGRKIC VRQALDKTQ KGANNMNKQ MPNRRQGPP 
      QHYNEGPYM PPPPHQFQI HPLPHQQGP RPHQQQGPP 
      PHQQQGPPP HQQQGPPPH QQQGPPPHQ QQQFQKPPE 
      EAPKPTPKP EPEKEQEQV PAAPLPNQP PPNMGPQKP 
      PMVQEEPPK KEAPIERRG RKRRMRSRE RFSSDMYRE 
      DGYYPSGKY SNRPPVIDQ PDRNDCEII VVSKQLTKY 
      AEYIEQRLK ALGLIVDLL FPNEDVPIG RVLANISSR 
      GCLYAILVM PQNEEHRSL TLNILHGNP QEHRNMPIE 
      DALVLMTRN FDAYMRGDK TASEPTPGA IKSVSDRHP 
      TAMQLLLNL LAENRQLTT VQYDRLMKY LQERREIQH 
      QHEVSEGVD QDSQESNSK QAELQSRIM NILNKASTE 
      IQPPAQEPT PSNDVAPPL LKDPSVQKA LDSLLGGEM 
      FKNIGVV
    CDS sequence
    • >TC000027 RA
      atgtatcgc accgataaa cagttcatg accaatcct 
      gcaacagcc ccctcgcgc atctacatc gggggcatc 
      cccaagaca gtggtcgca gccgacttg gaagaaaaa 
      ttcaaagta cacggaaaa atcctaggc cttgtcctc 
      caaatgggc ttcgctttc atccaattc gaaaacgag 
      agccaatcg catgccgcg attcaaaac gaaaacaac 
      accatgctc cacggcaga aaaatctgc gtgagacaa 
      gcgttagac aagacccaa aaaggggca aacaatatg 
      aataaacaa atgccaaat cggagacaa ggcccccct 
      cagcactac aacgagggg ccttacatg cccccgccc 
      ccacaccag ttccaaatc cacccgctc ccacatcaa 
      caggggccg cgcccacat caacagcaa gggccgcct 
      ccgcatcaa cagcaagga ccacccccg catcagcaa 
      caggggccg cctccacat caacagcag gggccaccg 
      ccacatcaa cagcaacag ttccagaag cccccagaa 
      gaggccccg aagcccacc cccaaaccc gaaccggag 
      aaagagcaa gagcaagtc ccagctgcg ccgcttcca 
      aaccagccg ccgcctaat atggggccc caaaaaccg 
      ccaatggtg caggaggag cccccgaag aaggaggcg 
      ccgatagag aggagaggg cggaagaga aggatgcgg 
      agtcgggag cggttttcg tcagacatg tacagggag 
      gacgggtat tatccttcg gggaagtac tcaaatcgg 
      ccgcctgtt attgaccaa cctgacaga aatgactgc 
      gaaattatt gtcgtctcg aagcagttg acgaaatac 
      gccgagtat attgaacag agactgaaa gccttggga 
      ctcatcgtg gatttgctg tttccaaac gaggatgtc 
      cccattggc cgagtgttg gcgaatatt tcaagcaga 
      ggctgtttg tatgcaatt ttggtcatg ccgcaaaat 
      gaggaacac agaagtttg actcttaat attttacat 
      gggaatccc caagagcac cggaatatg ccaatagaa 
      gatgctttg gtcctaatg acacgaaat tttgatgct 
      tatatgcgt ggagataaa acagcgtca gaaccgacc 
      cctggggct attaaaagt gtttctgac agacatcca 
      acagcaatg cagttgctg ttgaatttg ctggctgaa 
      aaccgccag ttaacaaca gtccagtac gaccgattg 
      atgaagtac ttacaagag cgccgcgaa attcaacac 
      caacacgaa gtaagtgaa ggagtcgat caagactca 
      caagaatct aacagcaaa caagccgaa cttcaaagt 
      cgcatcatg aacatatta aataaagct tccactgag 
      attcaaccg ccagctcaa gaaccgaca ccgtcaaat 
      gacgttgca ccgccactt ctgaaagat ccttccgtt 
      caaaaggcg ctggatagt cttttaggt ggtgaaatg 
      tttaaaaat attggtgtg gtgtaa
    mRNA sequence
    • >TC000027 RA
      aatcggtgt taatcgcaa gtattttct gttcgttta 
      ttgtgtttt gtgatttgc gtttcgtta ggcaaccaa 
      ccaaactgt gatggcatt tgatgccaa aacaacaga 
      gtaaggtta tgtacaatt gtgtttgaa tatttgtat 
      atttatgga ttttctgta ttttaaaag tacatatct 
      gtgattgtg ggttcactt ttgcaggtg tagtgttac 
      tttgtgtgc cgggaaatg taataaaac gcaattttg 
      tccgaaaaa aattgtgcg cctagaatc aacattcct 
      actccacaa gtctcaaat ctgcttaaa agttaatac 
      aattcaaga tgtatcgca ccgataaac agttcatga 
      ccaatcctg caacagccc cctcgcgca tctacatcg 
      ggggcatcc ccaagacag tggtcgcag ccgacttgg 
      aagaaaaat tcaaagtac acggaaaaa tcctaggcc 
      ttgtcctcc aaatgggct tcgctttca tccaattcg 
      aaaacgaga gccaatcgc atgccgcga ttcaaaacg 
      aaaacaaca ccatgctcc acggcagaa aaatctgcg 
      tgagacaag cgttagaca agacccaaa aaggggcaa 
      acaatatga ataaacaaa tgccaaatc ggagacaag 
      gcccccctc agcactaca acgaggggc cttacatgc 
      ccccgcccc cacaccagt tccaaatcc acccgctcc 
      cacatcaac aggggccgc gcccacatc aacagcaag 
      ggccgcctc cgcatcaac agcaaggac cacccccgc 
      atcagcaac aggggccgc ctccacatc aacagcagg 
      ggccaccgc cacatcaac agcaacagt tccagaagc 
      ccccagaag aggccccga agcccaccc ccaaacccg 
      aaccggaga aagagcaag agcaagtcc cagctgcgc 
      cgcttccaa accagccgc cgcctaata tggggcccc 
      aaaaaccgc caatggtgc aggaggagc ccccgaaga 
      aggaggcgc cgatagaga ggagagggc ggaagagaa 
      ggatgcgga gtcgggagc ggttttcgt cagacatgt 
      acagggagg acgggtatt atccttcgg ggaagtact 
      caaatcggc cgcctgtta ttgaccaac ctgacagaa 
      atgactgcg aaattattg tcgtctcga agcagttga 
      cgaaatacg ccgagtata ttgaacaga gactgaaag 
      ccttgggac tcatcgtgg atttgctgt ttccaaacg 
      aggatgtcc ccattggcc gagtgttgg cgaatattt 
      caagcagag gctgtttgt atgcaattt tggtcatgc 
      cgcaaaatg aggaacaca gaagtttga ctcttaata 
      ttttacatg ggaatcccc aagagcacc ggaatatgc 
      caatagaag atgctttgg tcctaatga cacgaaatt 
      ttgatgctt atatgcgtg gagataaaa cagcgtcag 
      aaccgaccc ctggggcta ttaaaagtg tttctgaca 
      gacatccaa cagcaatgc agttgctgt tgaatttgc 
      tggctgaaa accgccagt taacaacag tccagtacg 
      accgattga tgaagtact tacaagagc gccgcgaaa 
      ttcaacacc aacacgaag taagtgaag gagtcgatc 
      aagactcac aagaatcta acagcaaac aagccgaac 
      ttcaaagtc gcatcatga acatattaa ataaagctt 
      ccactgaga ttcaaccgc cagctcaag aaccgacac 
      cgtcaaatg acgttgcac cgccacttc tgaaagatc 
      cttccgttc aaaaggcgc tggatagtc ttttaggtg 
      gtgaaatgt ttaaaaata ttggtgtgg tgtaaaatt 
      cgtcaaata aaatgtcct actcgac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY