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Gene information
General information
TC000026 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 15892564..15910652
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000026
    iBeetle screen iB_02963

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000026 RA
      MDLAETMKN VLAKGIQQT NVSDLPTST SMGFPGTGY 
      VTEKLYMLL QLYLQNKGW NSSVELLQC FSELKESSM 
      LPSAAYLQM MASRVTLDS QGRLILREN GKIILPFEH 
      FANAVMLKH MNGPHGLHL GVEATVRAV MESYTIGRE 
      NFGMEKEFI IEVVQNCPN PACRYYKNQ MELTQKTLQ 
      HLTTPPYLG EPQSNNPDI RSRLGAVDL TGQNNLDSK 
      ISQQLLDRP KSVAPTQQQ ITAALIQQQ NRVIAQQNI 
      DKLNANLEK QRQQIQLQQ QMDMAKGQQ KSHFDLPLM 
      DHKLTDFLR ANLENLEGL SAANKDLLA LHNGAWTSA 
      TSLTSSDDK RSSEGGQEK IVRAFSEVM KNMQRMKTY 
      VRPAMCKPY GKQSEALQK TLMDTIQLI QSLRSFLPP 
      PHIQVSSWK NEDKHRYEE T
    CDS sequence
    • >TC000026 RA
      atggatctg gcagagacc atgaaaaat gtcttagcc 
      aaagggatt cagcaaacg aacgtcagt gatttaccc 
      acatcgacc agcatgggc tttcctggt acaggttat 
      gttactgaa aaattgtac atgctgctc cagctgtac 
      ctgcaaaac aagggctgg aattcgtcc gttgagctt 
      ttgcagtgc ttttcggag ctgaaggaa tcgtccatg 
      ctaccaagt gctgcttat ttacaaatg atggcttca 
      agagttact ctagattcg caaggtaga ctgattttg 
      agagaaaat ggtaaaatt atacttccc tttgagcat 
      tttgccaat gctgtgatg ctgaaacat atgaatggg 
      cctcatggg ttgcatctt ggggttgag gcaactgta 
      agagctgtc atggaatct tacacaatc ggtagagaa 
      aatttcggc atggaaaaa gaattcata atcgaagta 
      gttcagaac tgtccaaat cccgcttgc cgctattac 
      aaaaatcaa atggaactg acccaaaag actttacag 
      catttaaca acacctcca tatttaggg gaaccccag 
      tcaaacaat ccggacatc cgcagtcgt ttgggcgca 
      gttgaccta acaggacag aataattta gactcaaaa 
      atcagtcaa cagctccta gataggcct aaatcagta 
      gctccaacc cagcaacag atcactgcg gcattaatc 
      cagcaacaa aatcgcgtc atcgcacaa caaaacatt 
      gataaattg aacgcaaat ttagaaaaa cagaggcaa 
      caaatccag cttcagcaa caaatggat atggcgaaa 
      gggcagcag aagtcgcat ttcgacctg cctttgatg 
      gatcacaaa ctgaccgat tttttaagg gccaatttg 
      gagaattta gagggttta tcagcagct aataaagac 
      ttactggct ttgcataac ggggcctgg acttcggcc 
      acaagcctg acttcttca gatgataag aggtcgtca 
      gagggcggg caggagaaa attgttagg gcttttagc 
      gaagtgatg aagaatatg caaaggatg aaaacttac 
      gtcagacca gcgatgtgc aaaccgtat ggaaaacaa 
      tccgaagct ctacagaag accctaatg gacaccatt 
      cagctaatc cagtcgttg cgcagcttt ttgccgcca 
      ccccatatc caggtgagt tcatggaag aacgaggat 
      aaacatcgc tacgaagag acttaa
    mRNA sequence
    • >TC000026 RA
      agacagcca ccgcaacaa ggcccaaaa ttatgaaaa 
      tttaatgat tttacgagc acttcatta tgcgcagtc 
      gcaaacgat gaagccgaa agctacgaa tcccaagtg 
      atgttctta aaaaaatca gctgtgttt cagacaaaa 
      ccccaacta gctaatatg ttccggatg tgaattaaa 
      cagcctaag caggaaata atgccgttt taatccgcc 
      gatttttac gattctcgc accatggat ctggcagag 
      accatgaaa aatgtctta gccaaaggg attcagcaa 
      acgaacgtc agtgattta cccacatcg accagcatg 
      ggctttcct ggtacaggt tatgttact gaaaaattg 
      tacatgctg ctccagctg tacctgcaa aacaagggc 
      tggaattcg tccgttgag cttttgcag tgcttttcg 
      gagctgaag gaatcgtcc atgctacca agtgctgct 
      tatttacaa atgatggct tcaagagtt actctagat 
      tcgcaaggt agactgatt ttgagagaa aatggtaaa 
      attatactt ccctttgag cattttgcc aatgctgtg 
      atgctgaaa catatgaat gggcctcat gggttgcat 
      cttggggtt gaggcaact gtaagagct gtcatggaa 
      tcttacaca atcggtaga gaaaatttc ggcatggaa 
      aaagaattc ataatcgaa gtagttcag aactgtcca 
      aatcccgct tgccgctat tacaaaaat caaatggaa 
      ctgacccaa aagacttta cagcattta acaacacct 
      ccatattta ggggaaccc cagtcaaac aatccggac 
      atccgcagt cgtttgggc gcagttgac ctaacagga 
      cagaataat ttagactca aaaatcagt caacagctc 
      ctagatagg cctaaatca gtagctcca acccagcaa 
      cagatcact gcggcatta atccagcaa caaaatcgc 
      gtcatcgca caacaaaac attgataaa ttgaacgca 
      aatttagaa aaacagagg caacaaatc cagcttcag 
      caacaaatg gatatggcg aaagggcag cagaagtcg 
      catttcgac ctgcctttg atggatcac aaactgacc 
      gatttttta agggccaat ttggagaat ttagagggt 
      ttatcagca gctaataaa gacttactg gctttgcat 
      aacggggcc tggacttcg gccacaagc ctgacttct 
      tcagatgat aagaggtcg tcagagggc gggcaggag 
      aaaattgtt agggctttt agcgaagtg atgaagaat 
      atgcaaagg atgaaaact tacgtcaga ccagcgatg 
      tgcaaaccg tatggaaaa caatccgaa gctctacag 
      aagacccta atggacacc attcagcta atccagtcg 
      ttgcgcagc tttttgccg ccaccccat atccaggtg 
      agttcatgg aagaacgag gataaacat cgctacgaa 
      gagacttaa tgtaagctg ttttactag aaattttct 
      cattgacaa ggcagcgtt acatataac attttcata 
      aaacatgac ttccgagat tagattatc ctttagaag 
      gatataaaa taatgtgaa agtattttg ttccttatt 
      ttatatata gatatatta atgtaagtt tttatattt 
      tgttggttt tttagtgag tgttgaaat ttatcgaaa 
      ttaatggcc cacagtatc gtcgttttt tactcattt 
      tatgttaat cgtagtgtt agttgtttt atttttcat 
      gttttcaaa agtcagtac aatacgctg caagcgtga 
      cggaataga aagtataaa tgtgctctc tttattaac 
      tttacaaat tacgaaatt tattttatg tgatagcgt 
      cgtacttaa cacaatgag attgtttac tgagatgca 
      ttttatgtt gtgattatg ttttctcga tagtatttc 
      ggaaataac agtatttaa ttatttagt tgcagactt 
      gaactaagt acccgtgtt taagtatag tgtttgaat 
      taggtcatt gcaatattg ttacaattc gaaaagctg 
      ctctatggc caggctaca atatacgag ggttgaaga 
      tctagtcat taataattt aaaagaaaa gaaaattca 
      aaattttag ctgtgatat tttaattgg ccatgatat 
      tctaaaagt atagttttt cattccagt atttactat 
      ttcgatacc aaaaaattg tattactag tttcgctag 
      atttcgtgt agattatga tcgacaatt tgcatatca 
      ccttttatt tatttcctt gcgaatatt attgtagat 
      ctgagccag tatttgtaa acatgttcg aagactgtt 
      gtgaaatta tcattctta gttgtataa aatgatagg 
      attttagtg gtttttgta tgttgtcac gaaatagga 
      cttacctct aaggctata atataatct tgccaatat 
      tttataatt tcgaagctt agcttgttt gtttggctt 
      atttgatta ttatgtcat aggtaatgt atgtgatgc 
      taatattgt tgaaaacag atttctttt gttgtttgt 
      attcaggct tttgtgcaa taaaacgaa ctaaaagta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY