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Gene information
General information
TC000020 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450990.1: 3956..5073
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000020
    iBeetle screen iB_02961

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000020 RA
      MLDEPSLSN ITDPNFGRP PESQNVLLQ QLGHPHVSS 
      FNYMINQGL DQAISDLNP VEFMVNGDK ITLEITDAS 
      LSCPLVPMG TVGVKSPKV FPSECRQRA ATYKGRFIA 
      RVNWAINGE RQTAFDKDM GQLPIMIKS NKCHLSAMS 
      PAELVKHGE HEQEWGGYF VVKGHERLV RMLLMTRRN 
      YPIAIKRSG WKARGSIFS DCGISMRCV REDQTATTN 
      VLHFVTDGT AKLMFSYKK VLYYTPLVL ILKCLCDYC 
      DQFIYQKLI QGYQHDSYY LE
    CDS sequence
    • >TC000020 RA
      atgttggac gagccgagc ctttccaat atcacggat 
      cccaatttc gggcgcccc cccgagtca cagaacgtg 
      cttttgcaa caattgggg cacccacac gtgtctagc 
      ttcaattac atgataaat caggggcta gaccaggcg 
      atttctgat ttgaatccg gttgagttt atggtaaat 
      ggggataaa atcacactt gaaataacc gatgctagt 
      ctcagttgc cccttggtg ccgatgggc acagttggg 
      gtcaaaagc cctaaagta tttccatcg gagtgtagg 
      caaagggcc gcgacctac aaagggcgg tttattgcc 
      agggttaat tgggcgatt aatggggag agacagacg 
      gcgtttgat aaggatatg ggccagttg cccatcatg 
      attaaatcg aataagtgc cacttgagc gccatgtcc 
      cccgcagag ttggtcaaa cacggggag catgagcag 
      gaatggggg ggctatttt gtggtcaaa ggccacgag 
      agactagta aggatgttg ctaatgaca cgtcggaac 
      tatccaatt gctataaaa cgctcgggc tggaaagcg 
      cggggctcg attttttca gactgtggt atttccatg 
      aggtgtgtc agggaggac caaacggcc actacgaat 
      gttttacat tttgttacg gatggaacc gccaaatta 
      atgttcagt tacaagaaa gttttgtac tacacgccg 
      ttagtcctg attttgaaa tgtttgtgc gattattgc 
      gaccagttt atttaccag aagttgata caagggtac 
      cagcacgat tcctactac ttggagtaa
    mRNA sequence
    • >TC000020 RA
      tacgccacc gggaagtga caattgtcg ggttcaatt 
      ttcacgttt tattattta tttttaagt ttaagtgag 
      tgttttaat aatgttgga cgagccgag cctttccaa 
      tatcacgga tcccaattt cgggcgccc ccccgagtc 
      acagaacgt gcttttgca acaattggg gcacccaca 
      cgtgtctag cttcaatta catgataaa tcaggggct 
      agaccaggc gatttctga tttgaatcc ggttgagtt 
      tatggtaaa tggggataa aatcacact tgaaataac 
      cgatgctag tctcagttg ccccttggt gccgatggg 
      cacagttgg ggtcaaaag ccctaaagt atttccatc 
      ggagtgtag gcaaagggc cgcgaccta caaagggcg 
      gtttattgc cagggttaa ttgggcgat taatgggga 
      gagacagac ggcgtttga taaggatat gggccagtt 
      gcccatcat gattaaatc gaataagtg ccacttgag 
      cgccatgtc ccccgcaga gttggtcaa acacgggga 
      gcatgagca ggaatgggg gggctattt tgtggtcaa 
      aggccacga gagactagt aaggatgtt gctaatgac 
      acgtcggaa ctatccaat tgctataaa acgctcggg 
      ctggaaagc gcggggctc gattttttc agactgtgg 
      tatttccat gaggtgtgt cagggagga ccaaacggc 
      cactacgaa tgttttaca ttttgttac ggatggaac 
      cgccaaatt aatgttcag ttacaagaa agttttgta 
      ctacacgcc gttagtcct gattttgaa atgtttgtg 
      cgattattg cgaccagtt tatttacca gaagttgat 
      acaagggta ccagcacga ttcctacta cttggagta 
      agcataaat tgttactag agactggat ttttaggag 
      tta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY