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Gene information
General information
TC032688
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014824
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid30: 227680..238053
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032688
    iBeetle screen iB_02960
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_05703
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032688 RA
      MDVNIDGTN CPIKKTFFV VPKSPNDLD VYNWRLSFQ 
      EQERELLHN YKNMKSSIR LIKKIRDRH DHNIASYFI 
      KTVFMWELE NFDKNFWEQ SLSFVFMTM LRKYGEYIK 
      RRNIPNYWH EKHNLIEDL REETAVCIA NKIRNIGDH 
      IERICSTNS GFYDIVNYF LKSHEFDLD SFKQEVSVG 
      KKLLKRMES DKNLAQVST NSDDSETSD FAKLENLCL 
      QLASDQRKI VGLLERLGE DLEKKKTED ERIVRRIES 
      KIDRLLAKI TVEDFTR
    • >TC032688 RB
      MNNFFTGQK HEHSQTQDL VESFVKKTF FVVPKSPND 
      LDVYNWRLS FQEQERELL HNYKNMKSS IRLIKKIRD 
      RHDHNIASY FIKTVFMWE LENFDKNFW EQSLSFVFM 
      TMLRKYGEY IKRRNIPNY WHEKHNLIE DLREETAVC 
      IANKIRNIG DHIERICST NSGFYDIVN YFLKSHEFD 
      LDSFKQEVS VGKKLLKRM ESDKNLAQV STNSDDSET 
      SDFAKLENL CLQLASDQR KIVGLLERL GEDLEKKKT 
      EDERIVRRI ESKIDRLLA KITVEDFTR
    CDS sequence
    • >TC032688 RA
      atggatgtg aatatcgat ggtaccaat tgtcccatc 
      aagaaaact tttttcgtt gtcccgaaa agtccgaac 
      gatttggac gtttacaac tggagactg agcttccaa 
      gaacaagag cgtgaatta ctccataat tacaaaaac 
      atgaagtcg agcattaga ctaatcaag aaaatacga 
      gaccgacat gaccacaac attgccagc tattttatc 
      aagactgtc tttatgtgg gaactggaa aatttcgat 
      aaaaatttc tgggaacag tcgttaagt ttcgtgttt 
      atgactatg ttgagaaaa tacggtgag tatataaaa 
      cggcgcaac atcccgaac tattggcac gaaaaacac 
      aatctgatc gaagatttg agagaagaa actgctgtt 
      tgtatcgca aataaaatc cggaatatt ggcgaccac 
      atcgagcga atttgtagc acaaactcg ggtttttac 
      gacattgtt aattatttc ttaaagtcc cacgagttt 
      gacttggac agttttaaa caagaagtg agtgttggt 
      aaaaagctg ctcaaacga atggaatct gacaaaaat 
      ctggcacaa gtttcgaca aattctgac gattcggaa 
      acgtctgat tttgcaaaa ttggaaaat ttgtgcctt 
      caattggcc tcagatcag aggaagatt gtgggtttg 
      ctggaacgt ttgggggaa gatttggag aagaagaaa 
      acggaagat gaacgaatt gtgaggagg attgagtca 
      aaaattgac cgcttgttg gcgaaaata actgttgaa 
      gattttacg aggtga
    • >TC032688 RB
      atgaacaac ttcttcaca gggcaaaaa cacgaacac 
      tcacaaaca caagacctg gtggaaagc ttcgtcaaa 
      aaaactttt ttcgttgtc ccgaaaagt ccgaacgat 
      ttggacgtt tacaactgg agactgagc ttccaagaa 
      caagagcgt gaattactc cataattac aaaaacatg 
      aagtcgagc attagacta atcaagaaa atacgagac 
      cgacatgac cacaacatt gccagctat tttatcaag 
      actgtcttt atgtgggaa ctggaaaat ttcgataaa 
      aatttctgg gaacagtcg ttaagtttc gtgtttatg 
      actatgttg agaaaatac ggtgagtat ataaaacgg 
      cgcaacatc ccgaactat tggcacgaa aaacacaat 
      ctgatcgaa gatttgaga gaagaaact gctgtttgt 
      atcgcaaat aaaatccgg aatattggc gaccacatc 
      gagcgaatt tgtagcaca aactcgggt ttttacgac 
      attgttaat tatttctta aagtcccac gagtttgac 
      ttggacagt tttaaacaa gaagtgagt gttggtaaa 
      aagctgctc aaacgaatg gaatctgac aaaaatctg 
      gcacaagtt tcgacaaat tctgacgat tcggaaacg 
      tctgatttt gcaaaattg gaaaatttg tgccttcaa 
      ttggcctca gatcagagg aagattgtg ggtttgctg 
      gaacgtttg ggggaagat ttggagaag aagaaaacg 
      gaagatgaa cgaattgtg aggaggatt gagtcaaaa 
      attgaccgc ttgttggcg aaaataact gttgaagat 
      tttacgagg tga
    mRNA sequence
    • >TC032688 RA
      gaaatttga aaatggatg tgaatatcg atggtacca 
      attgtccca tcaagaaaa cttttttcg ttgtcccga 
      aaagtccga acgatttgg acgtttaca actggagac 
      tgagcttcc aagaacaag agcgtgaat tactccata 
      attacaaaa acatgaagt cgagcatta gactaatca 
      agaaaatac gagaccgac atgaccaca acattgcca 
      gctatttta tcaagactg tctttatgt gggaactgg 
      aaaatttcg ataaaaatt tctgggaac agtcgttaa 
      gtttcgtgt ttatgacta tgttgagaa aatacggtg 
      agtatataa aacggcgca acatcccga actattggc 
      acgaaaaac acaatctga tcgaagatt tgagagaag 
      aaactgctg tttgtatcg caaataaaa tccggaata 
      ttggcgacc acatcgagc gaatttgta gcacaaact 
      cgggttttt acgacattg ttaattatt tcttaaagt 
      cccacgagt ttgacttgg acagtttta aacaagaag 
      tgagtgttg gtaaaaagc tgctcaaac gaatggaat 
      ctgacaaaa atctggcac aagtttcga caaattctg 
      acgattcgg aaacgtctg attttgcaa aattggaaa 
      atttgtgcc ttcaattgg cctcagatc agaggaaga 
      ttgtgggtt tgctggaac gtttggggg aagatttgg 
      agaagaaga aaacggaag atgaacgaa ttgtgagga 
      ggattgagt caaaaattg accgcttgt tggcgaaaa 
      taactgttg aagatttta cgaggtgat ttagaaaaa 
      aaaaaagtg ttattttgt cgttttctc aagtctagg 
      caaattgta ttgataaag agggctgac attaggaaa 
      acagcttta ataaattat tttttttgt a
    • >TC032688 RB
      tagtagttg gtaaagagt attgtgata acaatttta 
      acaaaaact ctttaaaaa aattctcgg gtttaaaag 
      taaattgaa aaaaaatca ctaacgctt atcacattt 
      tgcactgtt tcaacactt aaataactt ggcttatgc 
      acgtcttca aattttgga ataaagcca aggattttg 
      ttcttgcac aataccact ctgtaattt ttaagttca 
      cacatagga atttcttta tcgacatca gttataacg 
      tatgcataa tcacgcatc ttctttaca aattcctca 
      aattcactt tttataaac gatgtagca gccgtcagt 
      tgaggattg ctaatttta ataactgga agataatct 
      tcaaatggt tttacacca acacttaaa aatttgctt 
      cctgatttt gttatcggt atcacagtc tcatccaaa 
      agtcgatat ttttcattt tttatttta ctcaattct 
      tctgccttc atattctcg tccatttca tccgcaaac 
      ttgctcata gccttctcc aattccaca tcttcaggt 
      aactctatc aaaagctca gaagaagaa gaaggtgaa 
      tcttgagac aaaaccatt aattgcgaa cagggaatt 
      tcacattta tactcgtca tccaacttc ggattttta 
      aacgcattt ggatattca gtttcttgc gacataaac 
      tccacttca atattcgac ttcccacgc tttcgaact 
      tgacggggc attcttctt tgattcatt tttctatat 
      attaaaaaa agttctatt atacacatt aatatttta 
      aataaaaat aatgtcaac ttacaattt atcaacctg 
      cgtaataca gacagattt tgccctcca aaaatatga 
      acaacttct tcacagggc aaaaacacg aacactcac 
      aaacacaag acctggtgg aaagcttcg tcaaaaaaa 
      cttttttcg ttgtcccga aaagtccga acgatttgg 
      acgtttaca actggagac tgagcttcc aagaacaag 
      agcgtgaat tactccata attacaaaa acatgaagt 
      cgagcatta gactaatca agaaaatac gagaccgac 
      atgaccaca acattgcca gctatttta tcaagactg 
      tctttatgt gggaactgg aaaatttcg ataaaaatt 
      tctgggaac agtcgttaa gtttcgtgt ttatgacta 
      tgttgagaa aatacggtg agtatataa aacggcgca 
      acatcccga actattggc acgaaaaac acaatctga 
      tcgaagatt tgagagaag aaactgctg tttgtatcg 
      caaataaaa tccggaata ttggcgacc acatcgagc 
      gaatttgta gcacaaact cgggttttt acgacattg 
      ttaattatt tcttaaagt cccacgagt ttgacttgg 
      acagtttta aacaagaag tgagtgttg gtaaaaagc 
      tgctcaaac gaatggaat ctgacaaaa atctggcac 
      aagtttcga caaattctg acgattcgg aaacgtctg 
      attttgcaa aattggaaa atttgtgcc ttcaattgg 
      cctcagatc agaggaaga ttgtgggtt tgctggaac 
      gtttggggg aagatttgg agaagaaga aaacggaag 
      atgaacgaa ttgtgagga ggattgagt caaaaattg 
      accgcttgt tggcgaaaa taactgttg aagatttta 
      cgaggtgat ttagaaaaa aaaaaagtg ttattttgt 
      cgttttctc aagtctagg caaattgta ttgataaag 
      agggctgac attaggaaa acagcttta ataaattat 
      tttttttgt a