Status
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Gene information
General information
TC032688
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014824 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 30620281..30630654
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032688
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02960

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_05703

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032688 RA
      MDVNIDGTN CPIKKTFFV VPKSPNDLD VYNWRLSFQ 
EQERELLHN YKNMKSSIR LIKKIRDRH DHNIASYFI 
KTVFMWELE NFDKNFWEQ SLSFVFMTM LRKYGEYIK 
RRNIPNYWH EKHNLIEDL REETAVCIA NKIRNIGDH 
IERICSTNS GFYDIVNYF LKSHEFDLD SFKQEVSVG 
KKLLKRMES DKNLAQVST NSDDSETSD FAKLENLCL 
QLASDQRKI VGLLERLGE DLEKKKTED ERIVRRIES 
KIDRLLAKI TVEDFTR
    • >TC032688 RB
      MNNFFTGQK HEHSQTQDL VESFVKKTF FVVPKSPND 
LDVYNWRLS FQEQERELL HNYKNMKSS IRLIKKIRD 
RHDHNIASY FIKTVFMWE LENFDKNFW EQSLSFVFM 
TMLRKYGEY IKRRNIPNY WHEKHNLIE DLREETAVC 
IANKIRNIG DHIERICST NSGFYDIVN YFLKSHEFD 
LDSFKQEVS VGKKLLKRM ESDKNLAQV STNSDDSET 
SDFAKLENL CLQLASDQR KIVGLLERL GEDLEKKKT 
EDERIVRRI ESKIDRLLA KITVEDFTR
    CDS sequence
    • >TC032688 RA
      atggatgtg aatatcgat ggtaccaat tgtcccatc 
aagaaaact tttttcgtt gtcccgaaa agtccgaac 
gatttggac gtttacaac tggagactg agcttccaa 
gaacaagag cgtgaatta ctccataat tacaaaaac 
atgaagtcg agcattaga ctaatcaag aaaatacga 
gaccgacat gaccacaac attgccagc tattttatc 
aagactgtc tttatgtgg gaactggaa aatttcgat 
aaaaatttc tgggaacag tcgttaagt ttcgtgttt 
atgactatg ttgagaaaa tacggtgag tatataaaa 
cggcgcaac atcccgaac tattggcac gaaaaacac 
aatctgatc gaagatttg agagaagaa actgctgtt 
tgtatcgca aataaaatc cggaatatt ggcgaccac 
atcgagcga atttgtagc acaaactcg ggtttttac 
gacattgtt aattatttc ttaaagtcc cacgagttt 
gacttggac agttttaaa caagaagtg agtgttggt 
aaaaagctg ctcaaacga atggaatct gacaaaaat 
ctggcacaa gtttcgaca aattctgac gattcggaa 
acgtctgat tttgcaaaa ttggaaaat ttgtgcctt 
caattggcc tcagatcag aggaagatt gtgggtttg 
ctggaacgt ttgggggaa gatttggag aagaagaaa 
acggaagat gaacgaatt gtgaggagg attgagtca 
aaaattgac cgcttgttg gcgaaaata actgttgaa 
gattttacg aggtga
    • >TC032688 RB
      atgaacaac ttcttcaca gggcaaaaa cacgaacac 
tcacaaaca caagacctg gtggaaagc ttcgtcaaa 
aaaactttt ttcgttgtc ccgaaaagt ccgaacgat 
ttggacgtt tacaactgg agactgagc ttccaagaa 
caagagcgt gaattactc cataattac aaaaacatg 
aagtcgagc attagacta atcaagaaa atacgagac 
cgacatgac cacaacatt gccagctat tttatcaag 
actgtcttt atgtgggaa ctggaaaat ttcgataaa 
aatttctgg gaacagtcg ttaagtttc gtgtttatg 
actatgttg agaaaatac ggtgagtat ataaaacgg 
cgcaacatc ccgaactat tggcacgaa aaacacaat 
ctgatcgaa gatttgaga gaagaaact gctgtttgt 
atcgcaaat aaaatccgg aatattggc gaccacatc 
gagcgaatt tgtagcaca aactcgggt ttttacgac 
attgttaat tatttctta aagtcccac gagtttgac 
ttggacagt tttaaacaa gaagtgagt gttggtaaa 
aagctgctc aaacgaatg gaatctgac aaaaatctg 
gcacaagtt tcgacaaat tctgacgat tcggaaacg 
tctgatttt gcaaaattg gaaaatttg tgccttcaa 
ttggcctca gatcagagg aagattgtg ggtttgctg 
gaacgtttg ggggaagat ttggagaag aagaaaacg 
gaagatgaa cgaattgtg aggaggatt gagtcaaaa 
attgaccgc ttgttggcg aaaataact gttgaagat 
tttacgagg tga
    mRNA sequence
    • >TC032688 RA
      gaaatttga aaatggatg tgaatatcg atggtacca 
attgtccca tcaagaaaa cttttttcg ttgtcccga 
aaagtccga acgatttgg acgtttaca actggagac 
tgagcttcc aagaacaag agcgtgaat tactccata 
attacaaaa acatgaagt cgagcatta gactaatca 
agaaaatac gagaccgac atgaccaca acattgcca 
gctatttta tcaagactg tctttatgt gggaactgg 
aaaatttcg ataaaaatt tctgggaac agtcgttaa 
gtttcgtgt ttatgacta tgttgagaa aatacggtg 
agtatataa aacggcgca acatcccga actattggc 
acgaaaaac acaatctga tcgaagatt tgagagaag 
aaactgctg tttgtatcg caaataaaa tccggaata 
ttggcgacc acatcgagc gaatttgta gcacaaact 
cgggttttt acgacattg ttaattatt tcttaaagt 
cccacgagt ttgacttgg acagtttta aacaagaag 
tgagtgttg gtaaaaagc tgctcaaac gaatggaat 
ctgacaaaa atctggcac aagtttcga caaattctg 
acgattcgg aaacgtctg attttgcaa aattggaaa 
atttgtgcc ttcaattgg cctcagatc agaggaaga 
ttgtgggtt tgctggaac gtttggggg aagatttgg 
agaagaaga aaacggaag atgaacgaa ttgtgagga 
ggattgagt caaaaattg accgcttgt tggcgaaaa 
taactgttg aagatttta cgaggtgat ttagaaaaa 
aaaaaagtg ttattttgt cgttttctc aagtctagg 
caaattgta ttgataaag agggctgac attaggaaa 
acagcttta ataaattat tttttttgt a
    • >TC032688 RB
      tagtagttg gtaaagagt attgtgata acaatttta 
acaaaaact ctttaaaaa aattctcgg gtttaaaag 
taaattgaa aaaaaatca ctaacgctt atcacattt 
tgcactgtt tcaacactt aaataactt ggcttatgc 
acgtcttca aattttgga ataaagcca aggattttg 
ttcttgcac aataccact ctgtaattt ttaagttca 
cacatagga atttcttta tcgacatca gttataacg 
tatgcataa tcacgcatc ttctttaca aattcctca 
aattcactt tttataaac gatgtagca gccgtcagt 
tgaggattg ctaatttta ataactgga agataatct 
tcaaatggt tttacacca acacttaaa aatttgctt 
cctgatttt gttatcggt atcacagtc tcatccaaa 
agtcgatat ttttcattt tttatttta ctcaattct 
tctgccttc atattctcg tccatttca tccgcaaac 
ttgctcata gccttctcc aattccaca tcttcaggt 
aactctatc aaaagctca gaagaagaa gaaggtgaa 
tcttgagac aaaaccatt aattgcgaa cagggaatt 
tcacattta tactcgtca tccaacttc ggattttta 
aacgcattt ggatattca gtttcttgc gacataaac 
tccacttca atattcgac ttcccacgc tttcgaact 
tgacggggc attcttctt tgattcatt tttctatat 
attaaaaaa agttctatt atacacatt aatatttta 
aataaaaat aatgtcaac ttacaattt atcaacctg 
cgtaataca gacagattt tgccctcca aaaatatga 
acaacttct tcacagggc aaaaacacg aacactcac 
aaacacaag acctggtgg aaagcttcg tcaaaaaaa 
cttttttcg ttgtcccga aaagtccga acgatttgg 
acgtttaca actggagac tgagcttcc aagaacaag 
agcgtgaat tactccata attacaaaa acatgaagt 
cgagcatta gactaatca agaaaatac gagaccgac 
atgaccaca acattgcca gctatttta tcaagactg 
tctttatgt gggaactgg aaaatttcg ataaaaatt 
tctgggaac agtcgttaa gtttcgtgt ttatgacta 
tgttgagaa aatacggtg agtatataa aacggcgca 
acatcccga actattggc acgaaaaac acaatctga 
tcgaagatt tgagagaag aaactgctg tttgtatcg 
caaataaaa tccggaata ttggcgacc acatcgagc 
gaatttgta gcacaaact cgggttttt acgacattg 
ttaattatt tcttaaagt cccacgagt ttgacttgg 
acagtttta aacaagaag tgagtgttg gtaaaaagc 
tgctcaaac gaatggaat ctgacaaaa atctggcac 
aagtttcga caaattctg acgattcgg aaacgtctg 
attttgcaa aattggaaa atttgtgcc ttcaattgg 
cctcagatc agaggaaga ttgtgggtt tgctggaac 
gtttggggg aagatttgg agaagaaga aaacggaag 
atgaacgaa ttgtgagga ggattgagt caaaaattg 
accgcttgt tggcgaaaa taactgttg aagatttta 
cgaggtgat ttagaaaaa aaaaaagtg ttattttgt 
cgttttctc aagtctagg caaattgta ttgataaag 
agggctgac attaggaaa acagcttta ataaattat 
tttttttgt a

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY