Status
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Gene information
General information
TC031479
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004710
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 889302..898288
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031479
    iBeetle screen iB_02959

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031479 RA
      MTAYITELS DMVPTCSAL ARKPDKLTI LRMAVAHMK 
      ALRGTGNTS ADGTYKPSF LTDQELKHL ILEAADGFL 
      FVVSCDTGR SDWYGSCLY DNIHPEDVE KVREQLSTQ 
      EPQNTGRIL DLKTGTVKK EGHQSSMRL CMGSRRGFI 
      CRMKVGNLT PENMAVGHL NRLKQRNSL GPGRDGQNY 
      AVVHCTGYI KNWPPTDMF TGVQMERQT EDEIHTSHC 
      CLVAIGRLQ VTSTPNTSD LSGSNSAAE FISRHSMDG 
      KFSFVDQRV IGLLGYSPP ELLGKSCFE FFHPEDQTH 
      MKDSFEQVL KLKGQVLSV MYRFRAKNR EWVWLRTSA 
      FAFLNPYTD DVEYIVCTN TTAKSLHSG QEGTTETEQ 
      VAYQQPGLD YSLQRRDAG LYSHMLPST HIQNPQQTA 
      RPSSGQNVY SNYEPTASP IAYGSPQAT ASSSSAGVL 
      GRLTKGSTT SPTPAAAAW TLRQAQPAA DGYQYNQPS 
      PRSPGPTYT QLSGGSRAS AAPGPSYQW NAWQGSSSQ 
      TSESGGPGS SGSGPPPQP QPHELSDML QMLDQGGAA 
      SFEDLNMFN TTFE
    CDS sequence
    • >TC031479 RA
      atgacggcc tacatcacg gagctgtcg gacatggtg 
      cccacctgc agcgccctc gcaaggaag cccgacaag 
      ctcacgatc ctccggatg gccgtagcc cacatgaag 
      gccctccga gggacgggc aataccagt gctgatgga 
      acgtacaaa ccttcattc ctgaccgat caggaattg 
      aagcatctt attttagaa gctgccgac gggttttta 
      ttcgttgta agctgtgat accggaaga agcgactgg 
      tacgggtcc tgcttatac gacaacatc caccctgaa 
      gacgtcgaa aaagtccga gaacagttg tcgacgcaa 
      gaacctcaa aacacgggg agaatcctc gatttgaag 
      actggtaca gtcaagaaa gaggggcac caatcctcg 
      atgcgcctc tgtatgggc tctcgtagg ggtttcatt 
      tgccgcatg aaagtcgga aacctgacg cccgaaaac 
      atggccgtg ggccacttg aatagattg aagcaacga 
      aatagttta gggcctggg agggacggt cagaattac 
      gccgtcgtt cactgtaca ggctatata aaaaactgg 
      ccgcccaca gacatgttc acaggagtg caaatggag 
      cgccagacc gaggacgag atccacact tcgcattgc 
      tgtcttgtt gccataggg cgcctccaa gtgacctca 
      actcccaac actagtgac ctgtcagga tcaaacagt 
      gcggccgag ttcatctcg cgacattcc atggacggc 
      aaatttagt ttcgtcgat caacgagtc atcggtttg 
      ctgggctac tccccgccc gaactctta ggaaagagc 
      tgcttcgag ttctttcat cccgaggat caaactcat 
      atgaaagac agtttcgaa caagttctg aagctaaag 
      ggacaagtc ctgtccgtc atgtacaga ttccgagcc 
      aaaaacagg gaatgggtg tggttgcgc accagcgct 
      ttcgccttc ctcaacccc tacaccgac gatgttgag 
      tacatcgtc tgtacaaac accacggcg aaatcgcta 
      cacagtggc caagagggc accactgaa accgagcaa 
      gttgcctat cagcagccg gggcttgat tacagcttg 
      cagcgcaga gacgcggga ctctactcg cacatgctg 
      ccctctaca cacattcaa aaccctcag cagacggcg 
      cgccctagc agcggccag aatgtatat agcaactac 
      gagccgacg gcgtccccc atcgcctac ggctcgccg 
      caagccacc gcgagcagc agcagcgcc ggggtgttg 
      ggccgcctg accaagggc agcaccacg agcccgacg 
      cccgccgcc gcggcttgg acgctgcgc caggcgcaa 
      ccggcggcc gacgggtac cagtacaac cagcccagc 
      ccgcggtcc cccggcccc acctacacg cagctgagt 
      ggcgggtcg cgggccagc gccgccccc ggcccctcc 
      taccagtgg aacgcctgg cagggcagc tccagccag 
      acctcggag tcggggggg cccggctcg agcggctcg 
      gggccgccc ccgcaaccg cagccccac gagctcagc 
      gacatgctg cagatgctc gatcaaggg ggcgccgct 
      tcgtttgag gatttgaac atgttcaac actaccttc 
      gagtag
    mRNA sequence
    • >TC031479 RA
      gttgtagtc aaaatggct aatggtcag ttgccaaaa 
      gtgggttct ctgacctcg tgcccgctt ccgcatggc 
      tttgttctt ggaagagta tctggtgcg ggtggtcgc 
      gatacatct cgcgggaga tttcgacgg ccggccaag 
      acggtgacg ttctccgag cgttggcat ttggagagc 
      gctgagcga gcgctgcat ccatcggag gtcggccag 
      ggccgtgca ttaaaaggg agcatcgtg ttcccgagc 
      agtcgtgac ccgccgcgg gggaggcga ccatcgacc 
      acagaacac agaccgtcg accagaggt tcccacgct 
      gccgagcct ccgtccccg cccgctcac ccagtggaa 
      caccggcga gctccgcgc tgcgctctc gcgatctga 
      tcgtactct cttacattt cctagtgtc ccaatcttc 
      gcgattttc aattacggc ccctagacg aactctttc 
      gcgagcaaa tcgattcgc caaagcgct atgggaagc 
      ggccctagg ccatcgttg ccactccac caatactct 
      tcgatccga catgcacga aacgagcca ttttttcac 
      gcagctata ctacatcat cggacgctc aaaaacttt 
      tcaatttgt cgcactttt ttacacact cactcaaaa 
      cggaccaac tgacgacag ccaatcaac aaccagcta 
      caggactca atcaacata gaattcgct ttttcctct 
      attttcatt taggtttcg tcttgttta ttttttaga 
      actttgact cggactttt gccggaacc aattcacgt 
      acttactac tcttgttta ttgtcatga gcacaattt 
      gtttccaaa ggtactaat caatagata cttgcaaca 
      aaatttttg aagactgtc caccacttt tgcttctta 
      ttgaatcat ccatctttg tgtgagata gtaacggaa 
      atttaattg gtctttttc tcttaaaat gttcagtta 
      aattgctaa atgtttcgg cttttttct aatggccat 
      cgtcaggca ataatttaa taattcatc acataatgg 
      tatcgagta attagatgg atattcata gtggcatct 
      ctattgtgg ctgtgaagg tgctgactt tgtgttttg 
      gcgtgcgct ctttcgcaa tttgtgcat aggtgttgt 
      ggtgttgtc aacaaaatt gaatttgat gctaggaca 
      ggtgttggt gcgtcgtca cgttgaaaa aatgtatga 
      tttgcaaaa atgcatata ttccgtata aaaatctcg 
      tgattgtgt ggattatca gcgagtggt aatgggaaa 
      cgagtatta catggctgg tgattaatt ataggtatt 
      aatcacttt atagtatga gatgggtgc aggcagtcc 
      gtttgaaat aagacttat agaattgca aatctattg 
      ttgtgtttt tcgaaatag ctatattta tttttatgt 
      ggtcaggca atatcgcaa cgatgataa tgcacaaag 
      ttcgtattt atagttgtg tgaaagagt aactgaaag 
      tggaatatg tcatggcca aagtagcga attccagcc 
      ctcccgaca cggaaggca aaaagagga tataaattg 
      agcaaattt gaaaattga agttgaacg ttttagaaa 
      gtgccaatc atcagcatg cgatatttt caaagcggt 
      tctgcatta ccactgcct actactccc tgatggtta 
      agtaatcgt gagaacttg ctgaaatat ttagcataa 
      atgttaatt gcctctatc agaaaagct cgcgtcaag 
      ccgaccgag ttattatgt tatttgttc actcgatta 
      ttgtgcgat ttttcgcat gacggcttc aaatcccga 
      atttcatta agtcggtaa agacgggtg tcggtggcg 
      atgctacat aatgggacc gagtagatg gtcggagtc 
      tgcgattat gtgtgcctt tgtaatctc cattacgac 
      cattagccg gtctgcggc tctgtggtt ctaccgccg 
      tagcggccg atgggccgc gactccacg tctgcaggg 
      gtaatcatt gttatttag tttctcctc aattattga 
      ttatttacc gcaatactg gtactttta gaacgccac 
      ggacaaact ctgatgaaa taacgtctt tactcatta 
      ctattaatc atcactgcc agctagggc gttaaaaca 
      ccgttaacg tacgaacgc taaataatt gccaacttg 
      ttattttca tacatgggt tatttgtct aattacaaa 
      caaaggcat taacgtgcc acgcttact tatcatcag 
      ggacaaccc accagccca tgctggaat tagaggaat 
      ttgctcatt ctcacacac ctcactata ctttaagcg 
      tccctcaag aaattttaa gaattttct tcgctgttt 
      tcaagaact agttcctat ttgcttgga aaacagtaa 
      tttcataag caccgaaag tcgattcgt caactccat 
      accttaaca ttaattcac ctatcatat attttgatc 
      aattttgag tttagaaaa aaacctctc aacacattg 
      aatttcgtt aattgtact tttttcttc cttgggatt 
      gcattatta cttaaatgc tttctgttc ttggcggtt 
      acagtagac ataatatga cgtactata tagtgatca 
      tccgtagca tcttcttga ctaattaag gaagtatat 
      ttttatgtc aacaagaga accaagtgt cctctatca 
      tattagaat cattcttaa aaataatta atgtgaaga 
      taaaagggt ggaagattg ggttgggta gtccgatcg 
      tagttgtag tactgtggt actggagtt tattgtgtg 
      tattatttt ggcgtaaat ggggtgagt tgttgcaag 
      ggcccgttt gcggcatga catcagtga tatcgattc 
      cggcaagtt agttttatg gtgtccgga taatttact 
      cggcggcaa agtcgtaaa acgttcaac ggaaatacc 
      ggtaatctc acttgacga accgaagcg tcgccgctg 
      tttggacct ataaagtgc aggacatga ggcgagtcc 
      tgaaatatt cagcgtttc cttttggta aagtctcgc 
      atgaagtct aactagttc taagtagtg atggtttac 
      ttgggaaaa aaaatgtaa tgacctgtg acttttctt 
      gcaacttac tgtagggag aatcattgc gaaattgaa 
      cgccggagg cggaacaag atgacggcc tacatcacg 
      gagctgtcg gacatggtg cccacctgc agcgccctc 
      gcaaggaag cccgacaag ctcacgatc ctccggatg 
      gccgtagcc cacatgaag gccctccga gggacgggc 
      aataccagt gctgatgga acgtacaaa ccttcattc 
      ctgaccgat caggaattg aagcatctt attttagaa 
      gctgccgac gggttttta ttcgttgta agctgtgat 
      accggaaga agcgactgg tacgggtcc tgcttatac 
      gacaacatc caccctgaa gacgtcgaa aaagtccga 
      gaacagttg tcgacgcaa gaacctcaa aacacgggg 
      agaatcctc gatttgaag actggtaca gtcaagaaa 
      gaggggcac caatcctcg atgcgcctc tgtatgggc 
      tctcgtagg ggtttcatt tgccgcatg aaagtcgga 
      aacctgacg cccgaaaac atggccgtg ggccacttg 
      aatagattg aagcaacga aatagttta gggcctggg 
      agggacggt cagaattac gccgtcgtt cactgtaca 
      ggctatata aaaaactgg ccgcccaca gacatgttc 
      acaggagtg caaatggag cgccagacc gaggacgag 
      atccacact tcgcattgc tgtcttgtt gccataggg 
      cgcctccaa gtgacctca actcccaac actagtgac 
      ctgtcagga tcaaacagt gcggccgag ttcatctcg 
      cgacattcc atggacggc aaatttagt ttcgtcgat 
      caacgagtc atcggtttg ctgggctac tccccgccc 
      gaactctta ggaaagagc tgcttcgag ttctttcat 
      cccgaggat caaactcat atgaaagac agtttcgaa 
      caagttctg aagctaaag ggacaagtc ctgtccgtc 
      atgtacaga ttccgagcc aaaaacagg gaatgggtg 
      tggttgcgc accagcgct ttcgccttc ctcaacccc 
      tacaccgac gatgttgag tacatcgtc tgtacaaac 
      accacggcg aaatcgcta cacagtggc caagagggc 
      accactgaa accgagcaa gttgcctat cagcagccg 
      gggcttgat tacagcttg cagcgcaga gacgcggga 
      ctctactcg cacatgctg ccctctaca cacattcaa 
      aaccctcag cagacggcg cgccctagc agcggccag 
      aatgtatat agcaactac gagccgacg gcgtccccc 
      atcgcctac ggctcgccg caagccacc gcgagcagc 
      agcagcgcc ggggtgttg ggccgcctg accaagggc 
      agcaccacg agcccgacg cccgccgcc gcggcttgg 
      acgctgcgc caggcgcaa ccggcggcc gacgggtac 
      cagtacaac cagcccagc ccgcggtcc cccggcccc 
      acctacacg cagctgagt ggcgggtcg cgggccagc 
      gccgccccc ggcccctcc taccagtgg aacgcctgg 
      cagggcagc tccagccag acctcggag tcggggggg 
      cccggctcg agcggctcg gggccgccc ccgcaaccg 
      cagccccac gagctcagc gacatgctg cagatgctc 
      gatcaaggg ggcgccgct tcgtttgag gatttgaac 
      atgttcaac actaccttc gagtagtta tattttatc 
      gtgcaactt cagcaattg tttttgaac tgattataa 
      gacccattg taatttttt ctttttgtt attggatgt 
      attgtcaat gtagtgcta aagtgtatt tattttgta 
      aattaaaca atacgattt tagtacact tatttatca 
      cgttatttc ggggtggga tggtggtga cctgtaccg 
      tgtctagtc aactgtgac tactgcgag ctacagtga 
      tacgcttcg tagtaggta attgttgag taaatactt 
      gagtttaga ttccgagaa acaagcaat ttgttctcc 
      gctttttct acttttttt gtaaattgg caattgtta 
      caagcgtgc catcaacaa aataaacta ttttgcctt 
      ac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY