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Gene information
General information
TC001759 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 7661829..7669844
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001759
    iBeetle screen iB_02957
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: 2 - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001759 RA
      MSGYHESSW HEGCLGRCG DCTTCHHSD DGLYKRFVT 
      VGSVRYGMR VGAVSPPPP GAGPRYRSQ CCWPAATAP 
      GYVSRIPLL TTTPINSFD IISCANRLR INEEATSEL 
      PQQTVRDHV LYSTKCLKN QLKRCALVR PQIYARLCE 
      NADEEEFNL DRLPRFRFG GNRTTTNKV RDFEKRANE 
      QRLRDLTDR LKRGPIPPP RTRHQPKTP PEEPVRVTP 
      LRSASFSQV DYSSDDKKY VRRRNPICD ATNQTVATL 
      PRSKNPTRC DNFKETQIT NEQPERDTD DNNSLKQSI 
      TRSNTISSG TPNTQEKKR DKLRRRKGI YISQWPNST 
      ENVIPQFVE DGDFASCSD TPKLLEEPP DDKSQITMW 
      TSPQEEPLS PEDNTTVPE WPKITRGMF LLRSDSLSE 
      GEPENNKQD QISSVPSDV SDCESRLSM GNDPCSPRR 
      YSKRPLRGP YGQMLEAEM KKPETGRKS QLSNDLKFL 
      EDLSLQNRD VKLKHRPRC DDSKGSCNN LSSPKQLLT 
      LSKRKVSAD SLVVENDQR LVVNHQRTT SSPSKLEGF 
      ANTEVSQEL LEQLLRGSS EQLATDANL QQKNDTRTH 
      VVVELYDNE RTYVEALQI LVTKYLEPL KSPENTNLL 
      DATLVDEIF YQIPFLLSH HQAFLEELK KRLEHWDIK 
      QKIGDVFLE MFSKSSVIE SYTNYVNNW KRAREIIKS 
      AQQSKPTFA RFLETTARE HKGKLALDS LLIKPIQKF 
      PKYELLLQR LIKHTDVNH PDHSLLLAA QKEVHDQLL 
      KINCTEREA LELEQLREI EGLIEGLIE LVSADRQYL 
      RHDLVSMAA GGGTRKERA LFLFSDLLL VTSIKRRSG 
      TIRRPTGQG SVTSTLEAN KYKLLMKIA LEDLEIVRS 
      KDDNLKQMM IEIENLSAD VGILNQINE LSWSLHCNH 
      TQLDDLIRD MLSSLNKQL MEQQNSDSQ LSCLELTLN 
      TSNGLENVS IVFSKPEKR ASWEESFNE AKHKLAISG 
      DRKPSPELL ATVPIRKTR AGLQFTCAA PTLGDNGKD 
      VWVCNSDGY VGQVCILSL QPEPTVTSC NGVCNARIL 
      CIASVPGVS YSSVRGNNN REDTEEHKT IQFDSSSSS 
      EEDDETDNE EVDEPQKIS EAPDVSSVD LDDIDSQQS 
      TIWLGTEDG CIHVYNSSD NIRIKKNKI RLQHGSSIL 
      CIIYLDNRV FVSLANGDV TIYDRDGSG WNVNSPTTV 
      TVGSTNMPV SKMLPNVGK LWCSCGNTI KVFNTQNLT 
      TENHFTVNN DSNKPISCI VLFGNGLWL SLQNSAIVK 
      CFHTQNFEH LCEVNVAPA VTKMLTSCD DIIRQHKAA 
      CLRVTSLLA CKDLLWIGT SAGVLLTMP LPHITSSTT 
      KLPNVPTVT GVSHGHTGH VRFLTYVEL NSGPGEGPQ 
      RNSYKGKPE QRSNSKLLV ISGGDGYED FRSSNLSEV 
      AGREDSTNH LLLWNI
    CDS sequence
    • >TC001759 RA
      atgtcgggc taccatgag tccagctgg cacgagggc 
      tgcttgggc cgctgcggc gactgcacc acgtgccac 
      cactccgac gacggcctc tacaaaagg ttcgtgacg 
      gtggggtcg gtgcggtac ggcatgcgg gtgggggca 
      gtgtcgccg ccgccgccg ggagcgggt ccgcgctac 
      cgctcccag tgctgctgg cccgcagcg actgctccc 
      ggctacgtc tcccggatc ccgctgctt accacgacg 
      cctattaat agcttcgat ataattagt tgcgcaaat 
      agactgagg atcaacgag gaggctacg tcagagctg 
      cctcagcag actgtgcgc gatcatgta ctgtactcc 
      accaagtgc ctgaagaac caactcaag cgctgtgct 
      ttggtaagg cctcagatt tatgcacga ttgtgtgaa 
      aacgccgac gaagaagag ttcaacctg gatcggctg 
      ccgagattc cgcttcggc ggaaatcgg accactacc 
      aataaagtc agagatttc gaaaagaga gccaacgag 
      caaagactc agagacttg acggacaga ctcaaaagg 
      gggccgata ccgcccccg aggacaaga caccagcct 
      aaaacaccg ccagaggag ccagtcaga gtaacacca 
      ctacgaagt gcctccttc tcacaagtg gactattcc 
      agtgatgat aaaaaatac gtcaggcga aggaaccca 
      atttgtgat gcgacaaac caaacagtt gcgactcta 
      cccagatct aaaaaccca acaagatgt gataatttc 
      aaagaaaca cagatcaca aacgaacaa cccgaacgt 
      gacaccgat gataataac tccttgaaa caatcaata 
      acgaggagc aacaccatc tcaagcgga actcccaac 
      acccaggag aaaaaacgc gataaactc cgccgacgc 
      aaaggcatc tacatctcg caatggccc aactccacc 
      gaaaacgta atcccccag ttcgtcgaa gacggcgat 
      tttgcctcg tgttcggac acccccaaa cttttggaa 
      gaacccccg gacgacaaa tcgcaaata acaatgtgg 
      acaagtccg caagaagaa cccttaagt cccgaagac 
      aacacgacg gtgccagaa tggcccaaa atcacccga 
      gggatgttc ctcctccgt tcggactcc ctctcagag 
      ggtgagccc gaaaacaac aaacaagac caaatttcg 
      tccgtaccg tccgacgtc tccgactgc gagagcaga 
      ctaagcatg gggaacgac ccctgcagc ccccggcgg 
      tactccaag cggcccctg cgaggccca tacggccaa 
      atgcttgaa gccgagatg aaaaaaccc gaaactggg 
      cgcaaaagc caactaagc aatgacctg aaattcctc 
      gaggacttg tcgttacaa aatcgggac gttaagttg 
      aaacaccgg ccaaggtgt gacgactcg aagggaagt 
      tgtaataat ttaagttcg ccgaaacag ttgctaacg 
      ttatcgaaa cggaaagtt agtgctgat agtttagtt 
      gtggagaat gatcaaagg ttggttgtt aatcaccaa 
      cgtaccact tcgagtccc tccaaattg gaggggttc 
      gccaatact gaagtttcg caggaattg ctggagcag 
      ctgttgagg ggaagttcg gaacaactt gccaccgat 
      gccaattta cagcagaaa aatgatacg agaacgcac 
      gtggttgtc gagctttac gataacgag aggacttac 
      gtcgaagca ttacagatt ctggtcacg aaatatttg 
      gaaccgctt aaaagcccc gaaaacacc aatctttta 
      gacgcgaca cttgttgat gaaatcttt tatcaaata 
      ccgtttttg ttaagccac catcaggct tttttggaa 
      gaattaaaa aaacgccta gagcactgg gacattaaa 
      cagaaaatc ggagatgtc tttttggag atgttttcg 
      aaaagttcg gtcattgag agttacact aactacgtt 
      aataattgg aagcgagcg agggagatt atcaagagt 
      gcccaacag tccaaaccg acttttgcc cgatttttg 
      gagacaaca gcaagggaa cacaaaggc aagttagcc 
      cttgattct ttattgatc aaaccgatc caaaaattt 
      ccaaaatac gagttgttg ctccaaaga ttgatcaaa 
      cacactgat gttaaccac ccggaccac agtttgttg 
      ctagctgcg caaaaagag gtccatgat caattgctg 
      aaaatcaat tgtactgaa agagaagct ctggagttg 
      gaacaactc cgggaaatt gaaggtttg atcgaaggt 
      ttaatcgag ttggtgtca gccgaccgc cagtatttg 
      cgacatgat ttagtcagt atggctgcg ggtggtggc 
      actcgcaaa gaacgggct ttgttcctc ttctcggat 
      ttgctcctt gtgaccagt attaagcgg cggagtgga 
      acaatccga agacccacc ggtcaaggc agcgtgaca 
      agtaccctc gaagcgaac aagtacaaa ttacttatg 
      aagatagct ttagaagat ttagaaata gtccgctcg 
      aaggacgac aatttgaaa cagatgatg atcgaaatt 
      gagaattta tccgcggat gtcggcatt ctgaaccaa 
      attaatgaa ttaagttgg tcgctacat tgtaatcat 
      actcaatta gacgatctc attagagat atgttgtca 
      tctttgaat aagcaatta atggagcaa caaaacagc 
      gactcgcag ctctcttgt ctcgagctc accctcaac 
      acatccaac ggcctggaa aatgtctcg atcgttttt 
      tcgaagcct gaaaaacgc gcctcctgg gaggagtcg 
      ttcaacgag gccaagcac aaattagca atatcgggt 
      gatagaaaa ccaagtccg gaactcctc gcaacggtt 
      ccgatccgc aagacaaga gccggtctc caattcacc 
      tgcgctgcg cctacttta ggcgacaat ggcaaagac 
      gtgtgggtt tgcaacagc gacggttat gtcggtcaa 
      gtgtgcatt ttgagccta caacctgag cccaccgta 
      acgtcgtgc aacggtgtc tgtaacgcg aggattttg 
      tgcattgcc tcagtccct ggtgtcagt tacagttct 
      gttagaggg aacaacaac cgggaggac accgaggaa 
      cacaagact atccaattc gatagtagc tcctccagt 
      gaggaggac gacgaaacg gacaatgag gaggtcgac 
      gagccccaa aaaatttca gaggctccg gacgtttca 
      agtgtcgat ttggacgac attgacagt caacagtcg 
      acgatttgg ttggggacg gaagatggc tgtatccac 
      gtttacaat tccagtgac aatattcgg attaagaaa 
      aacaaaata cggttacag cacggatcg tcaatactt 
      tgcataata tatttggat aatcgggtt tttgtctcg 
      ttggccaat ggcgacgtt acgatctac gaccgggat 
      ggatctggt tggaacgtc aattctccg acgactgtg 
      acagtgggt tccacgaac atgccggtg tcaaaaatg 
      ctcccaaac gttggaaaa ttgtggtgc agttgcggc 
      aacactatt aaagtcttc aacactcaa aacctaacc 
      acggaaaat cactttacg gtcaataac gacagtaat 
      aagccgatt tcgtgtata gtcctcttc gggaatggt 
      ctctggctg tctttgcaa aactcggca atcgtcaag 
      tgcttccat actcagaac ttcgagcat ttgtgtgag 
      gtgaacgta gcccccgct gtgaccaaa atgctaaca 
      agttgtgat gatataatc agacagcac aaagcggcc 
      tgtttgcgc gtgacgtct cttttagct tgtaaggac 
      ttgttgtgg atcggaact tcggctggg gttctgcta 
      acaatgcct ctgccccac ataacatcg tcaacgacg 
      aaactaccg aacgttccg actgtcaca ggggtgtct 
      catggccat acgggccat gtccgattt ttgacctac 
      gttgaactc aattcaggg cccggcgaa gggccgcag 
      aggaattcg tacaagggc aagccggag cagcgcagt 
      aattcgaaa ttacttgtg atatcggga ggagacggt 
      tatgaagat ttccgcagt tcgaattta tcagaagtg 
      gcaggacgg gaggactcc accaatcat ctcctactg 
      tggaacata tga
    mRNA sequence
    • >TC001759 RA
      gaaatgtag tgaaataaa ttcgagtga ttggagtta 
      attgtgtcc aatgaagac ggaagcgaa ttcgcggtg 
      caaccatga attcaagag cgcttcttt ggttgcttc 
      aggtgtctg gtggtacct gctggcact agtggtcac 
      caacaggtg gttttcagt tctgaaaag tgccaagga 
      agcaaatgg tcgtaaagt ggcttgagt tgagcggat 
      ttcaatgat acatttttc aagtagagt gtcccctcc 
      acgattaga aacgcgcta ttaaagtaa gtggccagt 
      caagcggcc gccattgcc tgctctccg tctacctgt 
      gtctcgatg gctgtccaa gtcctcacc atttggcgt 
      gaagatgcg agggggtga ctgggatgt cgggctacc 
      atgagtcca gctggcacg agggctgct tgggccgct 
      gcggcgact gcaccacgt gccaccact ccgacgacg 
      gcctctaca aaaggttcg tgacggtgg ggtcggtgc 
      ggtacggca tgcgggtgg gggcagtgt cgccgccgc 
      cgccgggag cgggtccgc gctaccgct cccagtgct 
      gctggcccg cagcgactg ctcccggct acgtctccc 
      ggatcccgc tgcttacca cgacgccta ttaatagct 
      tcgatataa ttagttgcg caaatagac tgaggatca 
      acgaggagg ctacgtcag agctgcctc agcagactg 
      tgcgcgatc atgtactgt actccacca agtgcctga 
      agaaccaac tcaagcgct gtgctttgg taaggcctc 
      agatttatg cacgattgt gtgaaaacg ccgacgaag 
      aagagttca acctggatc ggctgccga gattccgct 
      tcggcggaa atcggacca ctaccaata aagtcagag 
      atttcgaaa agagagcca acgagcaaa gactcagag 
      acttgacgg acagactca aaagggggc cgataccgc 
      ccccgagga caagacacc agcctaaaa caccgccag 
      aggagccag tcagagtaa caccactac gaagtgcct 
      ccttctcac aagtggact attccagtg atgataaaa 
      aatacgtca ggcgaagga acccaattt gtgatgcga 
      caaaccaaa cagttgcga ctctaccca gatctaaaa 
      acccaacaa gatgtgata atttcaaag aaacacaga 
      tcacaaacg aacaacccg aacgtgaca ccgatgata 
      ataactcct tgaaacaat caataacga ggagcaaca 
      ccatctcaa gcggaactc ccaacaccc aggagaaaa 
      aacgcgata aactccgcc gacgcaaag gcatctaca 
      tctcgcaat ggcccaact ccaccgaaa acgtaatcc 
      cccagttcg tcgaagacg gcgattttg cctcgtgtt 
      cggacaccc ccaaacttt tggaagaac ccccggacg 
      acaaatcgc aaataacaa tgtggacaa gtccgcaag 
      aagaaccct taagtcccg aagacaaca cgacggtgc 
      cagaatggc ccaaaatca cccgaggga tgttcctcc 
      tccgttcgg actccctct cagagggtg agcccgaaa 
      acaacaaac aagaccaaa tttcgtccg taccgtccg 
      acgtctccg actgcgaga gcagactaa gcatgggga 
      acgacccct gcagccccc ggcggtact ccaagcggc 
      ccctgcgag gcccatacg gccaaatgc ttgaagccg 
      agatgaaaa aacccgaaa ctgggcgca aaagccaac 
      taagcaatg acctgaaat tcctcgagg acttgtcgt 
      tacaaaatc gggacgtta agttgaaac accggccaa 
      ggtgtgacg actcgaagg gaagttgta ataatttaa 
      gttcgccga aacagttgc taacgttat cgaaacgga 
      aagttagtg ctgatagtt tagttgtgg agaatgatc 
      aaaggttgg ttgttaatc accaacgta ccacttcga 
      gtccctcca aattggagg ggttcgcca atactgaag 
      tttcgcagg aattgctgg agcagctgt tgaggggaa 
      gttcggaac aacttgcca ccgatgcca atttacagc 
      agaaaaatg atacgagaa cgcacgtgg ttgtcgagc 
      tttacgata acgagagga cttacgtcg aagcattac 
      agattctgg tcacgaaat atttggaac cgcttaaaa 
      gccccgaaa acaccaatc ttttagacg cgacacttg 
      ttgatgaaa tcttttatc aaataccgt ttttgttaa 
      gccaccatc aggcttttt tggaagaat taaaaaaac 
      gcctagagc actgggaca ttaaacaga aaatcggag 
      atgtctttt tggagatgt tttcgaaaa gttcggtca 
      ttgagagtt acactaact acgttaata attggaagc 
      gagcgaggg agattatca agagtgccc aacagtcca 
      aaccgactt ttgcccgat ttttggaga caacagcaa 
      gggaacaca aaggcaagt tagcccttg attctttat 
      tgatcaaac cgatccaaa aatttccaa aatacgagt 
      tgttgctcc aaagattga tcaaacaca ctgatgtta 
      accacccgg accacagtt tgttgctag ctgcgcaaa 
      aagaggtcc atgatcaat tgctgaaaa tcaattgta 
      ctgaaagag aagctctgg agttggaac aactccggg 
      aaattgaag gtttgatcg aaggtttaa tcgagttgg 
      tgtcagccg accgccagt atttgcgac atgatttag 
      tcagtatgg ctgcgggtg gtggcactc gcaaagaac 
      gggctttgt tcctcttct cggatttgc tccttgtga 
      ccagtatta agcggcgga gtggaacaa tccgaagac 
      ccaccggtc aaggcagcg tgacaagta ccctcgaag 
      cgaacaagt acaaattac ttatgaaga tagctttag 
      aagatttag aaatagtcc gctcgaagg acgacaatt 
      tgaaacaga tgatgatcg aaattgaga atttatccg 
      cggatgtcg gcattctga accaaatta atgaattaa 
      gttggtcgc tacattgta atcatactc aattagacg 
      atctcatta gagatatgt tgtcatctt tgaataagc 
      aattaatgg agcaacaaa acagcgact cgcagctct 
      cttgtctcg agctcaccc tcaacacat ccaacggcc 
      tggaaaatg tctcgatcg ttttttcga agcctgaaa 
      aacgcgcct cctgggagg agtcgttca acgaggcca 
      agcacaaat tagcaatat cgggtgata gaaaaccaa 
      gtccggaac tcctcgcaa cggttccga tccgcaaga 
      caagagccg gtctccaat tcacctgcg ctgcgccta 
      ctttaggcg acaatggca aagacgtgt gggtttgca 
      acagcgacg gttatgtcg gtcaagtgt gcattttga 
      gcctacaac ctgagccca ccgtaacgt cgtgcaacg 
      gtgtctgta acgcgagga ttttgtgca ttgcctcag 
      tccctggtg tcagttaca gttctgtta gagggaaca 
      acaaccggg aggacaccg aggaacaca agactatcc 
      aattcgata gtagctcct ccagtgagg aggacgacg 
      aaacggaca atgaggagg tcgacgagc cccaaaaaa 
      tttcagagg ctccggacg tttcaagtg tcgatttgg 
      acgacattg acagtcaac agtcgacga tttggttgg 
      ggacggaag atggctgta tccacgttt acaattcca 
      gtgacaata ttcggatta agaaaaaca aaatacggt 
      tacagcacg gatcgtcaa tactttgca taatatatt 
      tggataatc gggtttttg tctcgttgg ccaatggcg 
      acgttacga tctacgacc gggatggat ctggttgga 
      acgtcaatt ctccgacga ctgtgacag tgggttcca 
      cgaacatgc cggtgtcaa aaatgctcc caaacgttg 
      gaaaattgt ggtgcagtt gcggcaaca ctattaaag 
      tcttcaaca ctcaaaacc taaccacgg aaaatcact 
      ttacggtca ataacgaca gtaataagc cgatttcgt 
      gtatagtcc tcttcggga atggtctct ggctgtctt 
      tgcaaaact cggcaatcg tcaagtgct tccatactc 
      agaacttcg agcatttgt gtgaggtga acgtagccc 
      ccgctgtga ccaaaatgc taacaagtt gtgatgata 
      taatcagac agcacaaag cggcctgtt tgcgcgtga 
      cgtctcttt tagcttgta aggacttgt tgtggatcg 
      gaacttcgg ctggggttc tgctaacaa tgcctctgc 
      cccacataa catcgtcaa cgacgaaac taccgaacg 
      ttccgactg tcacagggg tgtctcatg gccatacgg 
      gccatgtcc gatttttga cctacgttg aactcaatt 
      cagggcccg gcgaagggc cgcagagga attcgtaca 
      agggcaagc cggagcagc gcagtaatt cgaaattac 
      ttgtgatat cgggaggag acggttatg aagatttcc 
      gcagttcga atttatcag aagtggcag gacgggagg 
      actccacca atcatctcc tactgtgga acatatgat 
      cacagaaaa ttcttgtct aatacgctg tccaacaac 
      caagtgcga tttgtatca tttttcgcc aggattctg 
      gcgttgggc agcggcata tgaatgtgg catatctga 
      accgttcta tgttcgagt gataaatgc atttttgta 
      aaatgtgtg atagtaata ttaggtttg tgcataacg 
      gcattgttt gtttgtggt ggtagtgtt aatttcgcc 
      taacttgat atgttccta atatgttta tttggttgc 
      gataattgc attgtaagc tgtttatga cacgattac 
      tttgaggac gcccagatg taatttaat gttattttt 
      ttaatgatg cctatattt ttccttaca tcgaacgaa 
      ctgttttgt aataataaa ttttttaaa acattgcag 
      cacgagaaa ttataattt atattattg ctcaatgat 
      gtgacaaat tctatacct gttggagaa atacagtat 
      ttattttta t