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Gene information
General information
TC004785 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 6832471..6835132
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004785
    iBeetle screen iB_02955
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004785 RA
      MMFSQLIRG TKALSKVSQ PVRNFGLVP 
      IVIEQTGRG ERSYDIYSR LLKERIICL 
      MGPINDPMS SLIVAQLLF LQSESTNKP 
      IHMYINSPG GSVTAGLGI YDTMQYILP 
      PIATWCVGQ AASMASLLL AAGSPGMRH 
      SLPHARIMI HQPSGGAQG QATDIKIQA 
      EEILKLKSQ INNLYVKHT GLALEKIER 
      SMERDMFMS PVEAQSFGI IDKILTSPP 
      KNKAGSEEE VSASGETEK K
    CDS sequence
    • >TC004785 RA
      atgatgttt tcacaactc ataaggggg accaaagct 
      ttatcaaaa gtgtctcaa ccagtcaga aacttcggt 
      ctcgtccct attgtgatt gaacaaaca ggtcggggg 
      gaacgatct tacgacata tactcccgt ttgttgaaa 
      gaacggatt atttgttta atgggccca ataaatgac 
      ccaatgagt tccctcatc gtagcccag ttgttgttc 
      ctccagtca gaatcaacg aacaaaccc atacatatg 
      tacattaat tcacctggg ggaagtgtc actgcagga 
      ttagggatt tatgacact atgcagtac atcctgcca 
      ccgatagca acatggtgt gtgggacaa gctgcaagt 
      atggcctca ctgctacta gctgcggga agccccggg 
      atgaggcac tcgctgccc cacgcccga atcatgatc 
      caccagccg tcggggggc gcgcagggc caagccacc 
      gatattaaa atccaggct gaggagatt cttaaactg 
      aagagccaa attaataat ttgtacgtg aagcatacg 
      ggccttgcc ttggaaaaa atcgagcgt agtatggaa 
      cgtgacatg tttatgagc cctgttgag gcccaaagt 
      ttcgggatt atcgataaa attttaaca agtccgccc 
      aaaaataag gcagggagt gaggaagaa gtgagcgca 
      agtggcgaa acggagaaa aaatga
    mRNA sequence
    • >TC004785 RA
      actgtccga atttagttt gttaaacta aatttttgg 
      tcatagcac tctttgtga aatcccttt tttcttaat 
      cttaatcag catgtgagg tacatgatg ttttcacaa 
      ctcataagg gggaccaaa gctttatca aaagtgtct 
      caaccagtc agaaacttc ggtctcgtc cctattgtg 
      attgaacaa acaggtcgg ggggaacga tcttacgac 
      atatactcc cgtttgttg aaagaacgg attatttgt 
      ttaatgggc ccaataaat gacccaatg agttccctc 
      atcgtagcc cagttgttg ttcctccag tcagaatca 
      acgaacaaa cccatacat atgtacatt aattcacct 
      gggggaagt gtcactgca ggattaggg atttatgac 
      actatgcag tacatcctg ccaccgata gcaacatgg 
      tgtgtggga caagctgca agtatggcc tcactgcta 
      ctagctgcg ggaagcccc gggatgagg cactcgctg 
      ccccacgcc cgaatcatg atccaccag ccgtcgggg 
      ggcgcgcag ggccaagcc accgatatt aaaatccag 
      gctgaggag attcttaaa ctgaagagc caaattaat 
      aatttgtac gtgaagcat acgggcctt gccttggaa 
      aaaatcgag cgtagtatg gaacgtgac atgtttatg 
      agccctgtt gaggcccaa agtttcggg attatcgat 
      aaaatttta acaagtccg cccaaaaat aaggcaggg 
      agtgaggaa gaagtgagc gcaagtggc gaaacggag 
      aaaaaatga tttgttttt gtattgtgc gatcaagaa 
      aatttgcga tctagtcat ccatgtcgg ctaaacaaa 
      gtatagggt ggtaagtga atgagtagt ccatcaagt 
      tgccgtttg atttacgtc aaacgttca tggatcact 
      agatgtcaa gtgtgcttg gtcgcttat gtttgaata 
      aaagacgtt tggttgcaa aagactgtt ttaatatag 
      aatatttta attatataa tgaaataca tatttaaaa 
      aaatcttat ataatattg caaatgata acattgttc 
      attaactta tcatttagt tatgatagt tatagtact 
      cttacatag gtagaacta catgcagag agctatctt 
      acttaagtc taaactagg ctaatatca tgataacat 
      gtatgcaac agactacaa ttgttgcat acgagcaat 
      aaattactt gaaatatac acgactatg atttagtca 
      ttcagtacc atgtggaat gtcgggagt atgaatatt 
      cccatccta atttatgga agagtcagt taatcgtac 
      ccaaaataa ctttatttt ttgaggaaa tgttcgaaa 
      aattatatt atgataaag cataactca ggtacttat 
      aatttttgc gaaattctt gatactcgc aatatgaat 
      gtctgggct acaatgttc gtattctta tcacaataa 
      ggaatgtat ctagtctag gtcatggct tgatctatg 
      tacatgtca taaaactct aatctatat acaatgagt 
      aatttcctt attcccaac cctcagatc atttacatg 
      attcaattt tgagatcat tggtcaaac tcgaccaaa 
      tagcgtcgg aaaaacaag ctaacaaag aaaacatct 
      aaaaaagaa aacaataaa ataaacatt gatccatgt 
      a