Status
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Gene information
General information
TC030888
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004779,TC004780
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6908596..6972898
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030888
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02954

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head capsule decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment partially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030888 RA
      MDRVLKRVG LSKRTQSEI KVQEQTASE EKTKIRRRR 
      RSKSRRRSS QESKKTRGL FNIDWESVK DCKSCKSRA 
      ITRRFCSKR CDNELYRSN SFKFERFIR GSEEASTLG 
      KKISLCDDY SLPVDNLGN RRRPNSIAV PSFTEWNIA 
      SPNEEKGSS EPSSDDEFF GHQHISVTK PQELNSPDS 
      IAEAILPQL SAHSPNRSD IKRHPSPYY YADLYKNKV 
      EESKTCPAK FRKEPVLQP KPSQDVLES NQLSQKIKS 
      SHRKYRKSF SLDNPNLDK PIRARSHSR LKYKKSSSV 
      DTQSTDSRD APNEAVMPP NRLTSPCAC LTPDDGLDD 
      EMTRQRHVY ETAFDCRIS KSDDDLDQI DKVSNHPVL 
      VQLNNAREN RPGPSADSN TPQNRRKVT LLCPKPSQE 
      DNNSVGAIS QKLQEMRLP EEASVVSGA LSLPLRGYT 
      PSPPSTAPL PTKFPGKEL VMNSIRSAP NLPSHPKSR 
      LKDLHLPVK SLRGRSPNS SSGSLQIQR SAFDIEFGN 
      SKLISEFKG RPSQPKEMK EWPLKRHKG TKDNILEFK 
      GRRRRHKYS STESMTASS SGGSMESIK SSTSEGNRS 
      TTSSDSRQS SSLSSHSSD SGGTKAYCG TQNSSFLAH 
      ANKLHILSP ISDKSSQEP ISETSDNNR NNNSQKCSP 
      EEAATPETM KVKRRLPQN KNLLNLGFH AGDLEIQGS 
      DSGISIQSR EGIKSRATS VDLRDLPFD MPKLRRRLR 
      IQPSTESSV SQASSSQSV IEADRQVSS FRPKLTLNL 
      GELDLRQRP GLGLSLVGL GMNLNNPEN PPTADVIDV 
      NLPLEKQGW FHGAITRVE AENVLRMLR EGSFLVRNS 
      ESTKQDYSL SLKSARGFM HMRIQKDGN DECYILGQF 
      SKPFHSIPE MIRHFCINR LPIRGAEHM CLLQPVIAQ 
      LL
    CDS sequence
    • >TC030888 RA
      atggacaga gtgttaaaa cgcgtgggg ctttcgaaa 
      agaactcaa tctgagata aaggtccaa gagcagacc 
      gctagtgag gaaaaaacg aaaatacgc cgccgccgc 
      aggagcaaa tcgcgacgg aggtctagt caggagagt 
      aaaaaaaca cgcggacta tttaatatt gattgggaa 
      tcggtaaaa gactgtaaa tcctgcaag tcgagggcg 
      atcacaagg cggttttgc agcaaaaga tgtgataat 
      gagctctat aggtccaac agtttcaag ttcgagcgg 
      ttcatcagg ggcagtgag gaggcctcc actctgggc 
      aaaaagatt tcgttgtgt gatgattat agcctacca 
      gttgacaat ttaggaaat aggaggaga cccaatagc 
      atcgcagtt cctagtttc accgaatgg aatatcgct 
      agtccaaat gaagaaaag ggttcttca gaaccaagc 
      tccgatgac gagttcttc gggcaccag cacatctct 
      gtgacgaag cctcaggaa ttgaattct ccggatagc 
      atcgccgag gctatcctt cctcaactc agtgctcac 
      tcccccaat cgcagcgac ataaaacgg cacccttcg 
      ccctattac tacgctgat ttatacaag aataaagtc 
      gaggagtcg aaaacttgt ccggcgaaa ttccgcaaa 
      gagccagtc cttcagccg aaaccgtct caggatgtt 
      ttagaatca aaccagttg agccaaaaa atcaaatcg 
      tcgcatagg aagtaccgg aaaagcttt agtctagat 
      aacccgaat ttggataaa ccaatccga gccaggtcg 
      cacagccgt ttgaagtat aaaaagagt tcgagtgtt 
      gacacacaa agcactgat tcaagggat gcgcctaat 
      gaggcggtt atgcctccg aatagactg acttcaccc 
      tgtgcttgt ctcaccccc gacgatggc ttagacgac 
      gaaatgaca cgccaaagg catgtatac gaaaccgct 
      ttcgactgt cgcattagc aagtctgat gacgattta 
      gatcagatt gacaaagta tcaaaccat cctgtcctc 
      gtacaactg aataacgcg agggaaaac cgacctggg 
      ccgtccgct gatagcaat acaccacaa aacaggcgg 
      aaagtcacg ctgctctgc cccaaaccg agccaagag 
      gacaataac tccgtcggc gccatctcc cagaagctg 
      caggaaatg cgtttgccc gaagaagcg tctgtggtt 
      tctggggct ttgtccttg cccttgcga ggctacaca 
      ccatcccct ccgtccacc gcccctctt ccgacgaag 
      ttccccggc aaagagctg gtaatgaac agtattcgt 
      agtgcccca aatttgcca tcgcatccg aaaagtcgc 
      ttaaaagac ttgcatctt cccgtaaag tctctccga 
      ggccgttcg cccaatagt agtagtggc agtttgcaa 
      atccagaga agtgctttc gacatcgag ttcggtaac 
      agcaaactc atctcggag ttcaaaggg cggccgagc 
      caacccaag gaaatgaaa gagtggcca ctcaagcgc 
      cataaaggg actaaggac aacattttg gagttcaag 
      ggccgtcgc aggagacac aaatactca agcaccgag 
      agcatgacg gccagcagc agcggcggc agcatggag 
      agcattaag agcagcacc agcgagggc aacagaagt 
      acaacgagc tccgacagc cggcagtcc tcctctctg 
      agctcccac agctcggac agcggcggg acaaaggcg 
      tactgcggc acccaaaac tccagcttc ctcgcccac 
      gccaacaag ctccacatt ctgagcccc atctcggac 
      aaatcatct caggaacca atctcagaa acctccgac 
      aacaatcgg aacaacaac tcgcagaag tgctccccg 
      gaagaggcc gccacgccc gaaacgatg aaagtcaag 
      cggcgtctg ccccagaac aagaacctg ctgaacctg 
      ggcttccac gccggcgac ctggagatc cagggctcc 
      gacagcggg atctcgatc caatcgcgc gaaggcatc 
      aaatcgcgt gccacttcg gttgatttg agggattta 
      ccgtttgat atgcccaaa ttgagacgg cggcttcga 
      atacagccg agcactgag tcaagtgtc tcgcaggcg 
      tcgtccagc caaagtgtc atcgaagct gacaggcaa 
      gtgtcttcg ttccggccg aagctgacc ctgaatttg 
      ggggagttg gacttgcgt cagcgtccg ggccttgga 
      ttatctctt gttggactt ggaatgaac ttaaataat 
      cccgaaaac cctcccaca gcggacgtc attgatgtc 
      aatttgcca ctggagaag caaggatgg ttccacggg 
      gctatcact cgcgtcgaa gcggagaac gtgctcagg 
      atgttacga gaagggagt tttttagtt cggaatagc 
      gaatcgacg aaacaggat tattcgctc tcgctgaag 
      agcgctcgt ggcttcatg cacatgagg atacagaag 
      gacggaaat gacgagtgc tacattttg ggacagttc 
      agtaaaccg tttcattcg attccggaa atgatccgc 
      catttttgc ataaaccgg ctgccaatc cgaggggcg 
      gaacacatg tgcttgctg cagccggtc attgcgcaa 
      ctcttgtga
    mRNA sequence
    • >TC030888 RA
      accacctcg tttgtccac ttaccagtg gcgtacgaa 
      aaaaattaa ttaattttt tatttcaac cattggctt 
      ggctcccca atgagtgag tcagcaccg gtttatcta 
      aaatctgtc aaagacacg tgctgttaa gggttgtta 
      atttgcatt taccctaaa tcgaaagca gctgagtgc 
      gcgagcgtt gcgcatctc gtcgcgctt ttacgacag 
      tagagagca caccgtgga aatggacag agtgttaaa 
      acgcgtggg gctttcgaa aagaactca atctgagat 
      aaaggtcca agagcagac cgctagtga ggaaaaaac 
      gaaaatacg ccgccgccg caggagcaa atcgcgacg 
      gaggtctag tcaggagag taaaaaaac acgcggact 
      atttaatat tgattggga atcggtaaa agactgtaa 
      atcctgcaa gtcgagggc gatcacaag gcggttttg 
      cagcaaaag atgtgataa tgagctcta taggtccaa 
      cagtttcaa gttcgagcg gttcatcag gggcagtga 
      ggaggcctc cactctggg caaaaagat ttcgttgtg 
      tgatgatta tagcctacc agttgacaa tttaggaaa 
      taggaggag acccaatag catcgcagt tcctagttt 
      caccgaatg gaatatcgc tagtccaaa tgaagaaaa 
      gggttcttc agaaccaag ctccgatga cgagttctt 
      cgggcacca gcacatctc tgtgacgaa gcctcagga 
      attgaattc tccggatag catcgccga ggctatcct 
      tcctcaact cagtgctca ctcccccaa tcgcagcga 
      cataaaacg gcacccttc gccctatta ctacgctga 
      tttatacaa gaataaagt cgaggagtc gaaaacttg 
      tccggcgaa attccgcaa agagccagt ccttcagcc 
      gaaaccgtc tcaggatgt tttagaatc aaaccagtt 
      gagccaaaa aatcaaatc gtcgcatag gaagtaccg 
      gaaaagctt tagtctaga taacccgaa tttggataa 
      accaatccg agccaggtc gcacagccg tttgaagta 
      taaaaagag ttcgagtgt tgacacaca aagcactga 
      ttcaaggga tgcgcctaa tgaggcggt tatgcctcc 
      gaatagact gacttcacc ctgtgcttg tctcacccc 
      cgacgatgg cttagacga cgaaatgac acgccaaag 
      gcatgtata cgaaaccgc tttcgactg tcgcattag 
      caagtctga tgacgattt agatcagat tgacaaagt 
      atcaaacca tcctgtcct cgtacaact gaataacgc 
      gagggaaaa ccgacctgg gccgtccgc tgatagcaa 
      tacaccaca aaacaggcg gaaagtcac gctgctctg 
      ccccaaacc gagccaaga ggacaataa ctccgtcgg 
      cgccatctc ccagaagct gcaggaaat gcgtttgcc 
      cgaagaagc gtctgtggt ttctggggc tttgtcctt 
      gcccttgcg aggctacac accatcccc tccgtccac 
      cgcccctct tccgacgaa gttccccgg caaagagct 
      ggtaatgaa cagtattcg tagtgcccc aaatttgcc 
      atcgcatcc gaaaagtcg cttaaaaga cttgcatct 
      tcccgtaaa gtctctccg aggccgttc gcccaatag 
      tagtagtgg cagtttgca aatccagag aagtgcttt 
      cgacatcga gttcggtaa cagcaaact catctcgga 
      gttcaaagg gcggccgag ccaacccaa ggaaatgaa 
      agagtggcc actcaagcg ccataaagg gactaagga 
      caacatttt ggagttcaa gggccgtcg caggagaca 
      caaatactc aagcaccga gagcatgac ggccagcag 
      cagcggcgg cagcatgga gagcattaa gagcagcac 
      cagcgaggg caacagaag tacaacgag ctccgacag 
      ccggcagtc ctcctctct gagctccca cagctcgga 
      cagcggcgg gacaaaggc gtactgcgg cacccaaaa 
      ctccagctt cctcgccca cgccaacaa gctccacat 
      tctgagccc catctcgga caaatcatc tcaggaacc 
      aatctcaga aacctccga caacaatcg gaacaacaa 
      ctcgcagaa gtgctcccc ggaagaggc cgccacgcc 
      cgaaacgat gaaagtcaa gcggcgtct gccccagaa 
      caagaacct gctgaacct gggcttcca cgccggcga 
      cctggagat ccagggctc cgacagcgg gatctcgat 
      ccaatcgcg cgaaggcat caaatcgcg tgccacttc 
      ggttgattt gagggattt accgtttga tatgcccaa 
      attgagacg gcggcttcg aatacagcc gagcactga 
      gtcaagtgt ctcgcaggc gtcgtccag ccaaagtgt 
      catcgaagc tgacaggca agtgtcttc gttccggcc 
      gaagctgac cctgaattt gggggagtt ggacttgcg 
      tcagcgtcc gggccttgg attatctct tgttggact 
      tggaatgaa cttaaataa tcccgaaaa ccctcccac 
      agcggacgt cattgatgt caatttgcc actggagaa 
      gcaaggatg gttccacgg ggctatcac tcgcgtcga 
      agcggagaa cgtgctcag gatgttacg agaagggag 
      ttttttagt tcggaatag cgaatcgac gaaacagga 
      ttattcgct ctcgctgaa gagcgctcg tggcttcat 
      gcacatgag gatacagaa ggacggaaa tgacgagtg 
      ctacatttt gggacagtt cagtaaacc gtttcattc 
      gattccgga aatgatccg ccatttttg cataaaccg 
      gctgccaat ccgaggggc ggaacacat gtgcttgct 
      gcagccggt cattgcgca actcttgtg ataagataa 
      ggttttgct tttcttata taagacgca catttatta 
      aagaaacga tctattttt tctgtaaaa cggttaaac 
      caaaggctt ccacatgag tagagaaaa tgagatttt 
      tatctacgg tgttatttg ttattgatc actctttag 
      ttggccgta tgagatacg gcgtcactt taattgtta 
      aatagcaat tagggtcca ggcgacctt tgaagggtg 
      gtccgggcc ctagtagat gtgcaatat ttcgttagc 
      ctactttgt tcagttttg tcttgtttt agtacggct 
      acagaaact atattgcca aagaaaatg actgtgaat 
      aaaatatac atacctata tattatata catttcggt 
      gtaagagac tagttttag gtctagcct taggtcaat 
      gttttagga ttagcattc taataagaa aacaaagca 
      aatataaaa gtactttaa cattatatt acatttcac 
      tcacattat ggctcattg cgacattat atctacttt 
      agcaagtat tgttatttt cgatttcta ttacttaat 
      ctttcaaaa attcttaaa actgttcta gacgctata 
      ttatttacc aatgtaata tagtttaga gactttgtg 
      atgaatgtg ttaataagg gactgaata aaagtacgt 
      aagaacc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY