| General information | |||
| TC030888
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 6908596..6972898 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC030888 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02954 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head capsule decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen segment partially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC030888 RA
MDRVLKRVG LSKRTQSEI KVQEQTASE EKTKIRRRR RSKSRRRSS QESKKTRGL FNIDWESVK DCKSCKSRA ITRRFCSKR CDNELYRSN SFKFERFIR GSEEASTLG KKISLCDDY SLPVDNLGN RRRPNSIAV PSFTEWNIA SPNEEKGSS EPSSDDEFF GHQHISVTK PQELNSPDS IAEAILPQL SAHSPNRSD IKRHPSPYY YADLYKNKV EESKTCPAK FRKEPVLQP KPSQDVLES NQLSQKIKS SHRKYRKSF SLDNPNLDK PIRARSHSR LKYKKSSSV DTQSTDSRD APNEAVMPP NRLTSPCAC LTPDDGLDD EMTRQRHVY ETAFDCRIS KSDDDLDQI DKVSNHPVL VQLNNAREN RPGPSADSN TPQNRRKVT LLCPKPSQE DNNSVGAIS QKLQEMRLP EEASVVSGA LSLPLRGYT PSPPSTAPL PTKFPGKEL VMNSIRSAP NLPSHPKSR LKDLHLPVK SLRGRSPNS SSGSLQIQR SAFDIEFGN SKLISEFKG RPSQPKEMK EWPLKRHKG TKDNILEFK GRRRRHKYS STESMTASS SGGSMESIK SSTSEGNRS TTSSDSRQS SSLSSHSSD SGGTKAYCG TQNSSFLAH ANKLHILSP ISDKSSQEP ISETSDNNR NNNSQKCSP EEAATPETM KVKRRLPQN KNLLNLGFH AGDLEIQGS DSGISIQSR EGIKSRATS VDLRDLPFD MPKLRRRLR IQPSTESSV SQASSSQSV IEADRQVSS FRPKLTLNL GELDLRQRP GLGLSLVGL GMNLNNPEN PPTADVIDV NLPLEKQGW FHGAITRVE AENVLRMLR EGSFLVRNS ESTKQDYSL SLKSARGFM HMRIQKDGN DECYILGQF SKPFHSIPE MIRHFCINR LPIRGAEHM CLLQPVIAQ LL
- >TC030888 RA
atggacaga gtgttaaaa cgcgtgggg ctttcgaaa agaactcaa tctgagata aaggtccaa gagcagacc gctagtgag gaaaaaacg aaaatacgc cgccgccgc aggagcaaa tcgcgacgg aggtctagt caggagagt aaaaaaaca cgcggacta tttaatatt gattgggaa tcggtaaaa gactgtaaa tcctgcaag tcgagggcg atcacaagg cggttttgc agcaaaaga tgtgataat gagctctat aggtccaac agtttcaag ttcgagcgg ttcatcagg ggcagtgag gaggcctcc actctgggc aaaaagatt tcgttgtgt gatgattat agcctacca gttgacaat ttaggaaat aggaggaga cccaatagc atcgcagtt cctagtttc accgaatgg aatatcgct agtccaaat gaagaaaag ggttcttca gaaccaagc tccgatgac gagttcttc gggcaccag cacatctct gtgacgaag cctcaggaa ttgaattct ccggatagc atcgccgag gctatcctt cctcaactc agtgctcac tcccccaat cgcagcgac ataaaacgg cacccttcg ccctattac tacgctgat ttatacaag aataaagtc gaggagtcg aaaacttgt ccggcgaaa ttccgcaaa gagccagtc cttcagccg aaaccgtct caggatgtt ttagaatca aaccagttg agccaaaaa atcaaatcg tcgcatagg aagtaccgg aaaagcttt agtctagat aacccgaat ttggataaa ccaatccga gccaggtcg cacagccgt ttgaagtat aaaaagagt tcgagtgtt gacacacaa agcactgat tcaagggat gcgcctaat gaggcggtt atgcctccg aatagactg acttcaccc tgtgcttgt ctcaccccc gacgatggc ttagacgac gaaatgaca cgccaaagg catgtatac gaaaccgct ttcgactgt cgcattagc aagtctgat gacgattta gatcagatt gacaaagta tcaaaccat cctgtcctc gtacaactg aataacgcg agggaaaac cgacctggg ccgtccgct gatagcaat acaccacaa aacaggcgg aaagtcacg ctgctctgc cccaaaccg agccaagag gacaataac tccgtcggc gccatctcc cagaagctg caggaaatg cgtttgccc gaagaagcg tctgtggtt tctggggct ttgtccttg cccttgcga ggctacaca ccatcccct ccgtccacc gcccctctt ccgacgaag ttccccggc aaagagctg gtaatgaac agtattcgt agtgcccca aatttgcca tcgcatccg aaaagtcgc ttaaaagac ttgcatctt cccgtaaag tctctccga ggccgttcg cccaatagt agtagtggc agtttgcaa atccagaga agtgctttc gacatcgag ttcggtaac agcaaactc atctcggag ttcaaaggg cggccgagc caacccaag gaaatgaaa gagtggcca ctcaagcgc cataaaggg actaaggac aacattttg gagttcaag ggccgtcgc aggagacac aaatactca agcaccgag agcatgacg gccagcagc agcggcggc agcatggag agcattaag agcagcacc agcgagggc aacagaagt acaacgagc tccgacagc cggcagtcc tcctctctg agctcccac agctcggac agcggcggg acaaaggcg tactgcggc acccaaaac tccagcttc ctcgcccac gccaacaag ctccacatt ctgagcccc atctcggac aaatcatct caggaacca atctcagaa acctccgac aacaatcgg aacaacaac tcgcagaag tgctccccg gaagaggcc gccacgccc gaaacgatg aaagtcaag cggcgtctg ccccagaac aagaacctg ctgaacctg ggcttccac gccggcgac ctggagatc cagggctcc gacagcggg atctcgatc caatcgcgc gaaggcatc aaatcgcgt gccacttcg gttgatttg agggattta ccgtttgat atgcccaaa ttgagacgg cggcttcga atacagccg agcactgag tcaagtgtc tcgcaggcg tcgtccagc caaagtgtc atcgaagct gacaggcaa gtgtcttcg ttccggccg aagctgacc ctgaatttg ggggagttg gacttgcgt cagcgtccg ggccttgga ttatctctt gttggactt ggaatgaac ttaaataat cccgaaaac cctcccaca gcggacgtc attgatgtc aatttgcca ctggagaag caaggatgg ttccacggg gctatcact cgcgtcgaa gcggagaac gtgctcagg atgttacga gaagggagt tttttagtt cggaatagc gaatcgacg aaacaggat tattcgctc tcgctgaag agcgctcgt ggcttcatg cacatgagg atacagaag gacggaaat gacgagtgc tacattttg ggacagttc agtaaaccg tttcattcg attccggaa atgatccgc catttttgc ataaaccgg ctgccaatc cgaggggcg gaacacatg tgcttgctg cagccggtc attgcgcaa ctcttgtga
- >TC030888 RA
accacctcg tttgtccac ttaccagtg gcgtacgaa aaaaattaa ttaattttt tatttcaac cattggctt ggctcccca atgagtgag tcagcaccg gtttatcta aaatctgtc aaagacacg tgctgttaa gggttgtta atttgcatt taccctaaa tcgaaagca gctgagtgc gcgagcgtt gcgcatctc gtcgcgctt ttacgacag tagagagca caccgtgga aatggacag agtgttaaa acgcgtggg gctttcgaa aagaactca atctgagat aaaggtcca agagcagac cgctagtga ggaaaaaac gaaaatacg ccgccgccg caggagcaa atcgcgacg gaggtctag tcaggagag taaaaaaac acgcggact atttaatat tgattggga atcggtaaa agactgtaa atcctgcaa gtcgagggc gatcacaag gcggttttg cagcaaaag atgtgataa tgagctcta taggtccaa cagtttcaa gttcgagcg gttcatcag gggcagtga ggaggcctc cactctggg caaaaagat ttcgttgtg tgatgatta tagcctacc agttgacaa tttaggaaa taggaggag acccaatag catcgcagt tcctagttt caccgaatg gaatatcgc tagtccaaa tgaagaaaa gggttcttc agaaccaag ctccgatga cgagttctt cgggcacca gcacatctc tgtgacgaa gcctcagga attgaattc tccggatag catcgccga ggctatcct tcctcaact cagtgctca ctcccccaa tcgcagcga cataaaacg gcacccttc gccctatta ctacgctga tttatacaa gaataaagt cgaggagtc gaaaacttg tccggcgaa attccgcaa agagccagt ccttcagcc gaaaccgtc tcaggatgt tttagaatc aaaccagtt gagccaaaa aatcaaatc gtcgcatag gaagtaccg gaaaagctt tagtctaga taacccgaa tttggataa accaatccg agccaggtc gcacagccg tttgaagta taaaaagag ttcgagtgt tgacacaca aagcactga ttcaaggga tgcgcctaa tgaggcggt tatgcctcc gaatagact gacttcacc ctgtgcttg tctcacccc cgacgatgg cttagacga cgaaatgac acgccaaag gcatgtata cgaaaccgc tttcgactg tcgcattag caagtctga tgacgattt agatcagat tgacaaagt atcaaacca tcctgtcct cgtacaact gaataacgc gagggaaaa ccgacctgg gccgtccgc tgatagcaa tacaccaca aaacaggcg gaaagtcac gctgctctg ccccaaacc gagccaaga ggacaataa ctccgtcgg cgccatctc ccagaagct gcaggaaat gcgtttgcc cgaagaagc gtctgtggt ttctggggc tttgtcctt gcccttgcg aggctacac accatcccc tccgtccac cgcccctct tccgacgaa gttccccgg caaagagct ggtaatgaa cagtattcg tagtgcccc aaatttgcc atcgcatcc gaaaagtcg cttaaaaga cttgcatct tcccgtaaa gtctctccg aggccgttc gcccaatag tagtagtgg cagtttgca aatccagag aagtgcttt cgacatcga gttcggtaa cagcaaact catctcgga gttcaaagg gcggccgag ccaacccaa ggaaatgaa agagtggcc actcaagcg ccataaagg gactaagga caacatttt ggagttcaa gggccgtcg caggagaca caaatactc aagcaccga gagcatgac ggccagcag cagcggcgg cagcatgga gagcattaa gagcagcac cagcgaggg caacagaag tacaacgag ctccgacag ccggcagtc ctcctctct gagctccca cagctcgga cagcggcgg gacaaaggc gtactgcgg cacccaaaa ctccagctt cctcgccca cgccaacaa gctccacat tctgagccc catctcgga caaatcatc tcaggaacc aatctcaga aacctccga caacaatcg gaacaacaa ctcgcagaa gtgctcccc ggaagaggc cgccacgcc cgaaacgat gaaagtcaa gcggcgtct gccccagaa caagaacct gctgaacct gggcttcca cgccggcga cctggagat ccagggctc cgacagcgg gatctcgat ccaatcgcg cgaaggcat caaatcgcg tgccacttc ggttgattt gagggattt accgtttga tatgcccaa attgagacg gcggcttcg aatacagcc gagcactga gtcaagtgt ctcgcaggc gtcgtccag ccaaagtgt catcgaagc tgacaggca agtgtcttc gttccggcc gaagctgac cctgaattt gggggagtt ggacttgcg tcagcgtcc gggccttgg attatctct tgttggact tggaatgaa cttaaataa tcccgaaaa ccctcccac agcggacgt cattgatgt caatttgcc actggagaa gcaaggatg gttccacgg ggctatcac tcgcgtcga agcggagaa cgtgctcag gatgttacg agaagggag ttttttagt tcggaatag cgaatcgac gaaacagga ttattcgct ctcgctgaa gagcgctcg tggcttcat gcacatgag gatacagaa ggacggaaa tgacgagtg ctacatttt gggacagtt cagtaaacc gtttcattc gattccgga aatgatccg ccatttttg cataaaccg gctgccaat ccgaggggc ggaacacat gtgcttgct gcagccggt cattgcgca actcttgtg ataagataa ggttttgct tttcttata taagacgca catttatta aagaaacga tctattttt tctgtaaaa cggttaaac caaaggctt ccacatgag tagagaaaa tgagatttt tatctacgg tgttatttg ttattgatc actctttag ttggccgta tgagatacg gcgtcactt taattgtta aatagcaat tagggtcca ggcgacctt tgaagggtg gtccgggcc ctagtagat gtgcaatat ttcgttagc ctactttgt tcagttttg tcttgtttt agtacggct acagaaact atattgcca aagaaaatg actgtgaat aaaatatac atacctata tattatata catttcggt gtaagagac tagttttag gtctagcct taggtcaat gttttagga ttagcattc taataagaa aacaaagca aatataaaa gtactttaa cattatatt acatttcac tcacattat ggctcattg cgacattat atctacttt agcaagtat tgttatttt cgatttcta ttacttaat ctttcaaaa attcttaaa actgttcta gacgctata ttatttacc aatgtaata tagtttaga gactttgtg atgaatgtg ttaataagg gactgaata aaagtacgt aagaacc
