Status
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Gene information
General information
TC030888
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004779,TC004780 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6908596..6972898
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC030888
    iBeetle screen iB_02954

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head capsule decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment partially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030888 RA
      MDRVLKRVG LSKRTQSEI KVQEQTASE EKTKIRRRR 
RSKSRRRSS QESKKTRGL FNIDWESVK DCKSCKSRA 
ITRRFCSKR CDNELYRSN SFKFERFIR GSEEASTLG 
KKISLCDDY SLPVDNLGN RRRPNSIAV PSFTEWNIA 
SPNEEKGSS EPSSDDEFF GHQHISVTK PQELNSPDS 
IAEAILPQL SAHSPNRSD IKRHPSPYY YADLYKNKV 
EESKTCPAK FRKEPVLQP KPSQDVLES NQLSQKIKS 
SHRKYRKSF SLDNPNLDK PIRARSHSR LKYKKSSSV 
DTQSTDSRD APNEAVMPP NRLTSPCAC LTPDDGLDD 
EMTRQRHVY ETAFDCRIS KSDDDLDQI DKVSNHPVL 
VQLNNAREN RPGPSADSN TPQNRRKVT LLCPKPSQE 
DNNSVGAIS QKLQEMRLP EEASVVSGA LSLPLRGYT 
PSPPSTAPL PTKFPGKEL VMNSIRSAP NLPSHPKSR 
LKDLHLPVK SLRGRSPNS SSGSLQIQR SAFDIEFGN 
SKLISEFKG RPSQPKEMK EWPLKRHKG TKDNILEFK 
GRRRRHKYS STESMTASS SGGSMESIK SSTSEGNRS 
TTSSDSRQS SSLSSHSSD SGGTKAYCG TQNSSFLAH 
ANKLHILSP ISDKSSQEP ISETSDNNR NNNSQKCSP 
EEAATPETM KVKRRLPQN KNLLNLGFH AGDLEIQGS 
DSGISIQSR EGIKSRATS VDLRDLPFD MPKLRRRLR 
IQPSTESSV SQASSSQSV IEADRQVSS FRPKLTLNL 
GELDLRQRP GLGLSLVGL GMNLNNPEN PPTADVIDV 
NLPLEKQGW FHGAITRVE AENVLRMLR EGSFLVRNS 
ESTKQDYSL SLKSARGFM HMRIQKDGN DECYILGQF 
SKPFHSIPE MIRHFCINR LPIRGAEHM CLLQPVIAQ 
LL
    CDS sequence
    • >TC030888 RA
      atggacaga gtgttaaaa cgcgtgggg ctttcgaaa 
agaactcaa tctgagata aaggtccaa gagcagacc 
gctagtgag gaaaaaacg aaaatacgc cgccgccgc 
aggagcaaa tcgcgacgg aggtctagt caggagagt 
aaaaaaaca cgcggacta tttaatatt gattgggaa 
tcggtaaaa gactgtaaa tcctgcaag tcgagggcg 
atcacaagg cggttttgc agcaaaaga tgtgataat 
gagctctat aggtccaac agtttcaag ttcgagcgg 
ttcatcagg ggcagtgag gaggcctcc actctgggc 
aaaaagatt tcgttgtgt gatgattat agcctacca 
gttgacaat ttaggaaat aggaggaga cccaatagc 
atcgcagtt cctagtttc accgaatgg aatatcgct 
agtccaaat gaagaaaag ggttcttca gaaccaagc 
tccgatgac gagttcttc gggcaccag cacatctct 
gtgacgaag cctcaggaa ttgaattct ccggatagc 
atcgccgag gctatcctt cctcaactc agtgctcac 
tcccccaat cgcagcgac ataaaacgg cacccttcg 
ccctattac tacgctgat ttatacaag aataaagtc 
gaggagtcg aaaacttgt ccggcgaaa ttccgcaaa 
gagccagtc cttcagccg aaaccgtct caggatgtt 
ttagaatca aaccagttg agccaaaaa atcaaatcg 
tcgcatagg aagtaccgg aaaagcttt agtctagat 
aacccgaat ttggataaa ccaatccga gccaggtcg 
cacagccgt ttgaagtat aaaaagagt tcgagtgtt 
gacacacaa agcactgat tcaagggat gcgcctaat 
gaggcggtt atgcctccg aatagactg acttcaccc 
tgtgcttgt ctcaccccc gacgatggc ttagacgac 
gaaatgaca cgccaaagg catgtatac gaaaccgct 
ttcgactgt cgcattagc aagtctgat gacgattta 
gatcagatt gacaaagta tcaaaccat cctgtcctc 
gtacaactg aataacgcg agggaaaac cgacctggg 
ccgtccgct gatagcaat acaccacaa aacaggcgg 
aaagtcacg ctgctctgc cccaaaccg agccaagag 
gacaataac tccgtcggc gccatctcc cagaagctg 
caggaaatg cgtttgccc gaagaagcg tctgtggtt 
tctggggct ttgtccttg cccttgcga ggctacaca 
ccatcccct ccgtccacc gcccctctt ccgacgaag 
ttccccggc aaagagctg gtaatgaac agtattcgt 
agtgcccca aatttgcca tcgcatccg aaaagtcgc 
ttaaaagac ttgcatctt cccgtaaag tctctccga 
ggccgttcg cccaatagt agtagtggc agtttgcaa 
atccagaga agtgctttc gacatcgag ttcggtaac 
agcaaactc atctcggag ttcaaaggg cggccgagc 
caacccaag gaaatgaaa gagtggcca ctcaagcgc 
cataaaggg actaaggac aacattttg gagttcaag 
ggccgtcgc aggagacac aaatactca agcaccgag 
agcatgacg gccagcagc agcggcggc agcatggag 
agcattaag agcagcacc agcgagggc aacagaagt 
acaacgagc tccgacagc cggcagtcc tcctctctg 
agctcccac agctcggac agcggcggg acaaaggcg 
tactgcggc acccaaaac tccagcttc ctcgcccac 
gccaacaag ctccacatt ctgagcccc atctcggac 
aaatcatct caggaacca atctcagaa acctccgac 
aacaatcgg aacaacaac tcgcagaag tgctccccg 
gaagaggcc gccacgccc gaaacgatg aaagtcaag 
cggcgtctg ccccagaac aagaacctg ctgaacctg 
ggcttccac gccggcgac ctggagatc cagggctcc 
gacagcggg atctcgatc caatcgcgc gaaggcatc 
aaatcgcgt gccacttcg gttgatttg agggattta 
ccgtttgat atgcccaaa ttgagacgg cggcttcga 
atacagccg agcactgag tcaagtgtc tcgcaggcg 
tcgtccagc caaagtgtc atcgaagct gacaggcaa 
gtgtcttcg ttccggccg aagctgacc ctgaatttg 
ggggagttg gacttgcgt cagcgtccg ggccttgga 
ttatctctt gttggactt ggaatgaac ttaaataat 
cccgaaaac cctcccaca gcggacgtc attgatgtc 
aatttgcca ctggagaag caaggatgg ttccacggg 
gctatcact cgcgtcgaa gcggagaac gtgctcagg 
atgttacga gaagggagt tttttagtt cggaatagc 
gaatcgacg aaacaggat tattcgctc tcgctgaag 
agcgctcgt ggcttcatg cacatgagg atacagaag 
gacggaaat gacgagtgc tacattttg ggacagttc 
agtaaaccg tttcattcg attccggaa atgatccgc 
catttttgc ataaaccgg ctgccaatc cgaggggcg 
gaacacatg tgcttgctg cagccggtc attgcgcaa 
ctcttgtga
    mRNA sequence
    • >TC030888 RA
      accacctcg tttgtccac ttaccagtg gcgtacgaa 
aaaaattaa ttaattttt tatttcaac cattggctt 
ggctcccca atgagtgag tcagcaccg gtttatcta 
aaatctgtc aaagacacg tgctgttaa gggttgtta 
atttgcatt taccctaaa tcgaaagca gctgagtgc 
gcgagcgtt gcgcatctc gtcgcgctt ttacgacag 
tagagagca caccgtgga aatggacag agtgttaaa 
acgcgtggg gctttcgaa aagaactca atctgagat 
aaaggtcca agagcagac cgctagtga ggaaaaaac 
gaaaatacg ccgccgccg caggagcaa atcgcgacg 
gaggtctag tcaggagag taaaaaaac acgcggact 
atttaatat tgattggga atcggtaaa agactgtaa 
atcctgcaa gtcgagggc gatcacaag gcggttttg 
cagcaaaag atgtgataa tgagctcta taggtccaa 
cagtttcaa gttcgagcg gttcatcag gggcagtga 
ggaggcctc cactctggg caaaaagat ttcgttgtg 
tgatgatta tagcctacc agttgacaa tttaggaaa 
taggaggag acccaatag catcgcagt tcctagttt 
caccgaatg gaatatcgc tagtccaaa tgaagaaaa 
gggttcttc agaaccaag ctccgatga cgagttctt 
cgggcacca gcacatctc tgtgacgaa gcctcagga 
attgaattc tccggatag catcgccga ggctatcct 
tcctcaact cagtgctca ctcccccaa tcgcagcga 
cataaaacg gcacccttc gccctatta ctacgctga 
tttatacaa gaataaagt cgaggagtc gaaaacttg 
tccggcgaa attccgcaa agagccagt ccttcagcc 
gaaaccgtc tcaggatgt tttagaatc aaaccagtt 
gagccaaaa aatcaaatc gtcgcatag gaagtaccg 
gaaaagctt tagtctaga taacccgaa tttggataa 
accaatccg agccaggtc gcacagccg tttgaagta 
taaaaagag ttcgagtgt tgacacaca aagcactga 
ttcaaggga tgcgcctaa tgaggcggt tatgcctcc 
gaatagact gacttcacc ctgtgcttg tctcacccc 
cgacgatgg cttagacga cgaaatgac acgccaaag 
gcatgtata cgaaaccgc tttcgactg tcgcattag 
caagtctga tgacgattt agatcagat tgacaaagt 
atcaaacca tcctgtcct cgtacaact gaataacgc 
gagggaaaa ccgacctgg gccgtccgc tgatagcaa 
tacaccaca aaacaggcg gaaagtcac gctgctctg 
ccccaaacc gagccaaga ggacaataa ctccgtcgg 
cgccatctc ccagaagct gcaggaaat gcgtttgcc 
cgaagaagc gtctgtggt ttctggggc tttgtcctt 
gcccttgcg aggctacac accatcccc tccgtccac 
cgcccctct tccgacgaa gttccccgg caaagagct 
ggtaatgaa cagtattcg tagtgcccc aaatttgcc 
atcgcatcc gaaaagtcg cttaaaaga cttgcatct 
tcccgtaaa gtctctccg aggccgttc gcccaatag 
tagtagtgg cagtttgca aatccagag aagtgcttt 
cgacatcga gttcggtaa cagcaaact catctcgga 
gttcaaagg gcggccgag ccaacccaa ggaaatgaa 
agagtggcc actcaagcg ccataaagg gactaagga 
caacatttt ggagttcaa gggccgtcg caggagaca 
caaatactc aagcaccga gagcatgac ggccagcag 
cagcggcgg cagcatgga gagcattaa gagcagcac 
cagcgaggg caacagaag tacaacgag ctccgacag 
ccggcagtc ctcctctct gagctccca cagctcgga 
cagcggcgg gacaaaggc gtactgcgg cacccaaaa 
ctccagctt cctcgccca cgccaacaa gctccacat 
tctgagccc catctcgga caaatcatc tcaggaacc 
aatctcaga aacctccga caacaatcg gaacaacaa 
ctcgcagaa gtgctcccc ggaagaggc cgccacgcc 
cgaaacgat gaaagtcaa gcggcgtct gccccagaa 
caagaacct gctgaacct gggcttcca cgccggcga 
cctggagat ccagggctc cgacagcgg gatctcgat 
ccaatcgcg cgaaggcat caaatcgcg tgccacttc 
ggttgattt gagggattt accgtttga tatgcccaa 
attgagacg gcggcttcg aatacagcc gagcactga 
gtcaagtgt ctcgcaggc gtcgtccag ccaaagtgt 
catcgaagc tgacaggca agtgtcttc gttccggcc 
gaagctgac cctgaattt gggggagtt ggacttgcg 
tcagcgtcc gggccttgg attatctct tgttggact 
tggaatgaa cttaaataa tcccgaaaa ccctcccac 
agcggacgt cattgatgt caatttgcc actggagaa 
gcaaggatg gttccacgg ggctatcac tcgcgtcga 
agcggagaa cgtgctcag gatgttacg agaagggag 
ttttttagt tcggaatag cgaatcgac gaaacagga 
ttattcgct ctcgctgaa gagcgctcg tggcttcat 
gcacatgag gatacagaa ggacggaaa tgacgagtg 
ctacatttt gggacagtt cagtaaacc gtttcattc 
gattccgga aatgatccg ccatttttg cataaaccg 
gctgccaat ccgaggggc ggaacacat gtgcttgct 
gcagccggt cattgcgca actcttgtg ataagataa 
ggttttgct tttcttata taagacgca catttatta 
aagaaacga tctattttt tctgtaaaa cggttaaac 
caaaggctt ccacatgag tagagaaaa tgagatttt 
tatctacgg tgttatttg ttattgatc actctttag 
ttggccgta tgagatacg gcgtcactt taattgtta 
aatagcaat tagggtcca ggcgacctt tgaagggtg 
gtccgggcc ctagtagat gtgcaatat ttcgttagc 
ctactttgt tcagttttg tcttgtttt agtacggct 
acagaaact atattgcca aagaaaatg actgtgaat 
aaaatatac atacctata tattatata catttcggt 
gtaagagac tagttttag gtctagcct taggtcaat 
gttttagga ttagcattc taataagaa aacaaagca 
aatataaaa gtactttaa cattatatt acatttcac 
tcacattat ggctcattg cgacattat atctacttt 
agcaagtat tgttatttt cgatttcta ttacttaat 
ctttcaaaa attcttaaa actgttcta gacgctata 
ttatttacc aatgtaata tagtttaga gactttgtg 
atgaatgtg ttaataagg gactgaata aaagtacgt 
aagaacc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY