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Gene information
General information
TC004775 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 6544671..6572713
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004775
    iBeetle screen iB_02953
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004775 RA
      MKISLGLFI CLLHGINCV KNAHLKKYL DERAKIVQE 
      QLIRHARNG ENPITVKLN NTNINLIGN YSHGAIFTL 
      FNTPYALLD QDLYEISPN NSTVIHVLP KKGTTFVKT 
      TQFQSLGFV ASASEQSLH ISGLHKDTR KLFKIQNIA 
      TNVTDATFF QNQNSLYLI TSHYKTHDL ALITIYKFI 
      ETHFDETVK FFASGTKIH TFPSDQSEI ILILHEKTS 
      SLFEFRDEG VKKIQKIDT KKPEQVLDY LHEGKRQVQ 
      IFGETSQLF LWTGTDLVL WEQFTESAG EQKLIFYVD 
      NVPYVLAQK NENVVLFTL SNNKFSPIY KSNFNYTKL 
      WSMAMNRNE LFTLGLVNN SVQVNEIVI SVTNNLAEK 
      LVDGHENSL NDCFLELEK TVYALRHQL TQTQNELSS 
      RAANSGGTV AQKYEKLRN KYASIKKTL SKPRSLIFD 
      EPAILRIPL KTKKLTAQK LKFDGLKGE KWRPERWLS 
      YSKPQNISG PVRAQMVQV TNLQTKQKL FDNLLLSTG 
      GQKLSGSVA TKYLETSQI VTDLLNEIP VDENIHTAA 
      INEIGGKKI AALLSQQLG RNFAPNLTI GNLWVENLN 
      EINFENLKN SVFRIGITD TISGNLTFA TLDSTKIQF 
      ETLSQKRDF LTTSTDQTI NSTITIPKI FTHDVKTAT 
      INGADFANL ATLNGTITG PVTIKDLNI RHNLELEGE 
      ISPEFLQIN NTHIVGTHE SDFFQRYDG RVKIAGNLY 
      LRDLELGKS AKMRLSGAE FEPEMGRFW SKSGQQEIP 
      THFEALNGV STPHVTTIF VNNVHVGDY MLNSTQQKR 
      KTKFYFDNV TIGGNVLLN PDEEHSPDL RRIQEESVR 
      INGTFSIRG RKTYKNVLK VHELELNRL NDVTISEGN 
      GKIQNLVVK GGLKSENLT VQTINGVNL TNLTANTLY 
      LNKAQNLSK LNFDKISVA NLFTSTLNN KSVAELVEK 
      VAKITNISK IENMVVTDH VTIKNAENL ATINDVKIE 
      QLMAKSHSR YHDGTIFGN VKFSGLVKI RDLQVVVIN 
      GVDFEKLSK RILYKSWEQ TINEHCVFN RLITDNLIT 
      DHINEFEVA NLIDVSSTK PQKIFARGG LKFAKNATI 
      KNLFSNTSC DLNEGLKNL RNPPSQLWD EITVTGNAF 
      FWDRESPIS DIFENVVSN YSKNHTIKG NVNFAHHVF 
      ANNITALKN VNNVNISEI IDDAFVNKP NLEQVITGQ 
      KIFQKLEAE NVEILQNTN VSSINKLNI SEIDAKIIK 
      KSANVAVKG NKVFFGGLE TKKIETEFI SGVEAKNLV 
      SFVNPTQVP NAMFRSVAV LRNFNVKFI NNMPLDVVL 
      KERVLKKGP KQVLTGNYF LQNVQFNGN ITTSRINDV 
      GMENVVYDN KNHAINSPK KFNHVVISG DMEAELVNG 
      FKLSQLYEQ SVLSNEETK FGKSLYVNK PSSMSGNIQ 
      TNYINNISV NYMLDTSKE NSLNSNTKQ IREITTIVT 
      NFIKTYFNS TMRMPTEVM YLEKSKDLD ISLPNVIGA 
      EVVETKNDL LIHIESAEE GPSCGLPPT CKCPTQQTM 
      QVSSKHSVN MFLNKATQR IYSHYDEDI VVHFITHSI 
      STDSYCSAN KDKVLDEVS SLTWTTTPR GNNTGGFFI 
      HRGFFTGYI TGVKFFTIY GKTYIFVGI SYDPISETH 
      AVDSLVLRF NEDRTKIEQ IQKIPTVGL KTISLFPTA 
      QGVVLVLGV DVNNTNTQI FRFNHHKEQ FELLRELPT 
      FDCVFAQGV VLEMDSLIV LANQRADLH ILKYQPEFD 
      SYYFYQSFE LDYPVSSLS VYYAGGFGI SDAYLSVVT 
      QNNKYFIFS FQFIAGWKL ESSGQLDGV KVLVPFRMD 
      KKSYLLAPS KTSSMLSVV EHGLD
    CDS sequence
    • >TC004775 RA
      atgaaaatt tccctcgga ttatttatt tgcttatta 
      catggcatc aactgcgtt aaaaacgct catttgaag 
      aagtatttg gacgaacga gcgaaaatt gtacaggag 
      caattaatt agacatgcc agaaacggg gaaaatccg 
      atcaccgtg aaacttaat aacacaaat ataaatctg 
      atcggaaat tatagccat ggagcgatt ttcactttg 
      ttcaatacg ccgtatgct ctactagac caagatttg 
      tacgaaatt tcaccaaat aattcaact gtaatccac 
      gttctccca aaaaaaggc accactttt gtcaagaca 
      actcagttt caatctctg ggctttgta gcatccgct 
      tcagaacag tcgctgcac atttcaggg ctccacaaa 
      gacactcga aaacttttc aaaatccaa aacattgca 
      accaacgta acggacgcc acgtttttc caaaaccaa 
      aacagcttg tacctgatc acctcgcac tacaagacg 
      cacgatctg gcattaata acaatttac aaatttatc 
      gaaacgcat ttcgacgaa actgtgaaa tttttcgct 
      tcgggaaca aaaatccac acttttccg agcgaccaa 
      agcgaaatt atcctaatc ttgcacgaa aaaacgtcg 
      agtttgttc gagtttcgt gacgagggt gttaaaaag 
      attcaaaaa attgacacc aaaaagccg gagcaagtt 
      ttggactat ttgcacgag ggaaaacgc caagttcag 
      atttttggc gaaactagc cagttgttt ttgtggacg 
      gggacggac ttggtgctt tgggagcaa ttcaccgaa 
      agtgcaggg gagcaaaaa ttgattttt tacgtggat 
      aatgtccca tatgttttg gcgcaaaaa aacgaaaat 
      gtcgtcctg tttacttta agtaacaac aaatttagt 
      ccaatttac aaatcgaat ttcaattat actaaatta 
      tggagcatg gcaatgaac agaaacgag ttatttact 
      ttaggatta gttaataat tcggtgcaa gtcaacgaa 
      atcgtaatt tcggtcaca aataatttg gcggaaaaa 
      ctcgtcgat ggacacgaa aattcgctg aatgattgt 
      ttcctggag ctagaaaaa actgtgtat gcactacgg 
      caccaactc acccaaact caaaacgag ttaagctca 
      agggctgca aacagtggt ggtacagtg gctcaaaaa 
      tacgaaaaa ctgcgaaat aaatacgcc agcattaaa 
      aaaacccta agcaaaccg agaagtttg atttttgac 
      gagcctgca attctccga attccccta aaaaccaaa 
      aaactcaca gcccagaaa ctcaaattc gatgggctt 
      aagggcgaa aaatggcgg cctgaaaga tggctgtcg 
      tactcaaaa cctcagaat atttcagga cctgtgagg 
      gcacaaatg gtgcaagtg actaatttg caaacgaaa 
      caaaaattg tttgataat ttgttgttg tcaacagga 
      gggcaaaaa ctgtcaggt tctgtcgct acaaagtat 
      ttggaaacg tcccaaatt gtgactgat ttactaaac 
      gagattccc gtcgatgaa aatattcac accgcggcc 
      ataaatgag attggtggg aaaaaaatt gcggcgctc 
      ctaagccag cagctgggg cgcaatttc gccccaaac 
      ctcaccatt ggcaattta tgggtcgaa aatttgaac 
      gaaattaat ttcgaaaat ttaaaaaat tctgttttt 
      cgaatcggt atcacggac acaatctct ggaaatctc 
      acattcgca accctcgac tcgaccaaa atccagttc 
      gaaactttg tcccaaaaa cgcgatttc ttgacgact 
      tccaccgac caaacgatt aactccaca atcaccatt 
      ccgaaaatt ttcacgcac gatgtcaaa acagcgaca 
      ataaacggg gccgatttt gcgaatttg gccacattg 
      aatggcaca ataaccggc ccggtgacc atcaaggac 
      ctgaacatc cgccacaac ttggagctt gagggcgag 
      atctccccc gagttcctg caaatcaac aacacgcac 
      atcgtgggg acgcacgag tcggacttt ttccagcgc 
      tacgacggc cgcgtgaaa atcgccgga aacctgtat 
      ttgcgcgac ctggagttg ggaaaatcg gcgaaaatg 
      cggctttcc ggggcggag tttgagccg gaaatgggc 
      cggttttgg agcaagtcc gggcagcag gagatcccg 
      acgcatttc gaggccctg aacggcgtc tcgacgccg 
      catgtgacc acgattttc gtcaacaac gtccacgtc 
      ggcgactac atgctgaat tcgacgcag cagaagcgc 
      aaaacgaag ttctacttc gataatgta acaatcgga 
      ggaaacgtg ttgctcaat ccggatgaa gaacattcg 
      cctgattta cggaggatt caagaagaa tcggttaga 
      attaatggg acgttcagt atcagaggc cgcaaaacg 
      tacaaaaac gtcctcaaa gtgcacgaa cttgagctt 
      aatcggctg aacgacgtt acgatcagt gaagggaac 
      gggaaaatt caaaatctc gttgttaaa ggtggcctc 
      aagtcggag aatttgacg gtgcaaaca attaacggc 
      gttaatcta actaattta accgccaac actttatac 
      ctcaacaag gcccaaaat ctctccaaa cttaacttt 
      gacaaaatt tcagttgcg aatttgttt acgtccaca 
      ttaaacaac aaaagtgta gccgaactg gtcgagaaa 
      gttgccaaa atcaccaac atttccaaa attgagaac 
      atggtcgtt actgaccat gtcacaatc aaaaacgct 
      gaaaacctg gcgacaatt aacgacgtc aaaattgag 
      caattaatg gcaaaaagt cactcgagg taccacgac 
      ggcacaatt tttggaaat gtcaaattt tcaggcctt 
      gtcaaaatt cgagatttg caggtcgtt gtcataaat 
      ggcgtcgat ttcgagaaa ttgtcaaaa cgaatttta 
      tacaagtcg tgggaacag acaatcaac gaacattgt 
      gtttttaac agactcatc acagataac ttaataact 
      gaccacata aacgaattt gaagtggca aacctgatc 
      gacgtttcg agcaccaaa ccccagaaa atttttgct 
      cgcggaggc ttaaaattc gcgaaaaac gccacaatc 
      aaaaatttg ttttcaaac acgagttgt gacctaaac 
      gagggtttg aagaactta cgaaaccct ccctcacaa 
      ctttgggac gagattaca gtcaccgga aacgcattt 
      ttctgggac agggaatca cccattagc gacattttt 
      gaaaatgtg gtcagcaat tacagcaaa aatcacaca 
      attaagggc aacgtgaat tttgcgcac catgttttc 
      gccaacaac ataacagct ttgaaaaat gtcaataac 
      gtgaacatt tcggaaatc atcgacgac gcgtttgtg 
      aacaaaccg aacttggag caggttata accgggcaa 
      aaaatcttc cagaagctg gaagccgaa aacgtcgag 
      attttgcaa aacacgaac gtttcgtca atcaacaaa 
      ctaaacatt tccgaaatc gacgctaaa attatcaaa 
      aaatcggcc aacgttgcg gttaaaggc aacaaagtg 
      tttttcggc ggcttggaa acgaaaaaa attgagact 
      gagtttata tcaggagtt gaggcgaaa aatttggtc 
      agttttgtg aaccccacc caggtgccc aacgcaatg 
      ttccgttca gtggctgtt ttgagaaat ttcaatgtc 
      aaatttatc aacaacatg ccccttgat gtagttctc 
      aaagaacga gttttgaaa aaagggccc aaacaagtc 
      ttgacaggg aactacttc ctccaaaat gtccaattc 
      aacggaaat ataacaacg tctcgaata aatgatgtg 
      gggatggaa aatgttgtt tatgataac aaaaatcac 
      gctattaat agtccgaag aaatttaac cacgttgtg 
      ataagtggg gacatggaa gcggagctt gttaatggg 
      ttcaagtta tcgcaattg tacgaacag tctgttttg 
      agtaatgag gagaccaag tttggaaaa tcactgtat 
      gtcaataaa ccaagttcc atgagtggc aacattcaa 
      acaaactac ataaacaac atttcggtc aattacatg 
      ctggacacg agtaaagaa aattctcta aattcaaac 
      actaaacaa atacgtgaa ataacaacc attgtgaca 
      aactttatc aaaacgtac tttaattcg acgatgagg 
      atgccaact gaagtaatg tatttggaa aaatcaaaa 
      gatttggac atttccctc ccgaatgta attggagcg 
      gaagttgtg gaaaccaaa aatgatctt ctaattcac 
      atcgagagt gcagaagag ggtccctct tgtggttta 
      cccccaact tgcaaatgc cccacccag caaacaatg 
      caagtttca agcaaacac tctgtaaat atgttcctg 
      aataaggcg acccagcga atatacagt cactatgac 
      gaagacatt gttgtgcat tttatcacc cacagcatc 
      agcactgat tcctactgc agtgcaaat aaggacaag 
      gtcctggac gaagtttcg tccctgact tggactacc 
      acaccccgt ggcaacaat acggggggt ttcttcatt 
      catcgcggc ttcttcaca ggatatata accggagtc 
      aagtttttc acaatttat ggcaaaacc tacatcttt 
      gttggaatt tcttacgac ccgatttcg gaaacacac 
      gccgttgac agtttagta ttgcggttt aatgaagat 
      cggacgaaa attgaacaa attcagaaa ataccaacc 
      gttggtttg aaaactatt tcattgttt ccgacagca 
      caaggggtg gtgctagtc ctaggggtt gatgttaat 
      aatacgaat acacaaatt ttccggttt aatcaccac 
      aaagaacag ttcgagctt ctgcgcgag ttacccact 
      tttgactgc gttttcgct caaggggtt gtgctcgaa 
      atggacagt ttgattgtc ttggccaat cagcgcgcg 
      gatttgcac attttgaaa tatcagccg gagtttgat 
      agttactat ttctatcaa agcttcgag ttggattat 
      ccggtctcc tcgctctct gtatattac gctggaggt 
      tttgggatt agcgatgcc tatttaagc gtcgtgacg 
      caaaacaac aaatatttc atcttttcg ttccagttt 
      atcgcaggt tggaagctc gaatcaagc ggtcagttg 
      gacggagtc aaggttttg gtaccgttc cgaatggac 
      aaaaaatcg taccttttg gctccgtct aaaacaagt 
      tcaatgctg tccgttgta gaacacggc ttggattaa
    mRNA sequence
    • >TC004775 RA
      caacgaatt tgccacaca atgaaaatt tccctcgga 
      ttatttatt tgcttatta catggcatc aactgcgtt 
      aaaaacgct catttgaag aagtatttg gacgaacga 
      gcgaaaatt gtacaggag caattaatt agacatgcc 
      agaaacggg gaaaatccg atcaccgtg aaacttaat 
      aacacaaat ataaatctg atcggaaat tatagccat 
      ggagcgatt ttcactttg ttcaatacg ccgtatgct 
      ctactagac caagatttg tacgaaatt tcaccaaat 
      aattcaact gtaatccac gttctccca aaaaaaggc 
      accactttt gtcaagaca actcagttt caatctctg 
      ggctttgta gcatccgct tcagaacag tcgctgcac 
      atttcaggg ctccacaaa gacactcga aaacttttc 
      aaaatccaa aacattgca accaacgta acggacgcc 
      acgtttttc caaaaccaa aacagcttg tacctgatc 
      acctcgcac tacaagacg cacgatctg gcattaata 
      acaatttac aaatttatc gaaacgcat ttcgacgaa 
      actgtgaaa tttttcgct tcgggaaca aaaatccac 
      acttttccg agcgaccaa agcgaaatt atcctaatc 
      ttgcacgaa aaaacgtcg agtttgttc gagtttcgt 
      gacgagggt gttaaaaag attcaaaaa attgacacc 
      aaaaagccg gagcaagtt ttggactat ttgcacgag 
      ggaaaacgc caagttcag atttttggc gaaactagc 
      cagttgttt ttgtggacg gggacggac ttggtgctt 
      tgggagcaa ttcaccgaa agtgcaggg gagcaaaaa 
      ttgattttt tacgtggat aatgtccca tatgttttg 
      gcgcaaaaa aacgaaaat gtcgtcctg tttacttta 
      agtaacaac aaatttagt ccaatttac aaatcgaat 
      ttcaattat actaaatta tggagcatg gcaatgaac 
      agaaacgag ttatttact ttaggatta gttaataat 
      tcggtgcaa gtcaacgaa atcgtaatt tcggtcaca 
      aataatttg gcggaaaaa ctcgtcgat ggacacgaa 
      aattcgctg aatgattgt ttcctggag ctagaaaaa 
      actgtgtat gcactacgg caccaactc acccaaact 
      caaaacgag ttaagctca agggctgca aacagtggt 
      ggtacagtg gctcaaaaa tacgaaaaa ctgcgaaat 
      aaatacgcc agcattaaa aaaacccta agcaaaccg 
      agaagtttg atttttgac gagcctgca attctccga 
      attccccta aaaaccaaa aaactcaca gcccagaaa 
      ctcaaattc gatgggctt aagggcgaa aaatggcgg 
      cctgaaaga tggctgtcg tactcaaaa cctcagaat 
      atttcagga cctgtgagg gcacaaatg gtgcaagtg 
      actaatttg caaacgaaa caaaaattg tttgataat 
      ttgttgttg tcaacagga gggcaaaaa ctgtcaggt 
      tctgtcgct acaaagtat ttggaaacg tcccaaatt 
      gtgactgat ttactaaac gagattccc gtcgatgaa 
      aatattcac accgcggcc ataaatgag attggtggg 
      aaaaaaatt gcggcgctc ctaagccag cagctgggg 
      cgcaatttc gccccaaac ctcaccatt ggcaattta 
      tgggtcgaa aatttgaac gaaattaat ttcgaaaat 
      ttaaaaaat tctgttttt cgaatcggt atcacggac 
      acaatctct ggaaatctc acattcgca accctcgac 
      tcgaccaaa atccagttc gaaactttg tcccaaaaa 
      cgcgatttc ttgacgact tccaccgac caaacgatt 
      aactccaca atcaccatt ccgaaaatt ttcacgcac 
      gatgtcaaa acagcgaca ataaacggg gccgatttt 
      gcgaatttg gccacattg aatggcaca ataaccggc 
      ccggtgacc atcaaggac ctgaacatc cgccacaac 
      ttggagctt gagggcgag atctccccc gagttcctg 
      caaatcaac aacacgcac atcgtgggg acgcacgag 
      tcggacttt ttccagcgc tacgacggc cgcgtgaaa 
      atcgccgga aacctgtat ttgcgcgac ctggagttg 
      ggaaaatcg gcgaaaatg cggctttcc ggggcggag 
      tttgagccg gaaatgggc cggttttgg agcaagtcc 
      gggcagcag gagatcccg acgcatttc gaggccctg 
      aacggcgtc tcgacgccg catgtgacc acgattttc 
      gtcaacaac gtccacgtc ggcgactac atgctgaat 
      tcgacgcag cagaagcgc aaaacgaag ttctacttc 
      gataatgta acaatcgga ggaaacgtg ttgctcaat 
      ccggatgaa gaacattcg cctgattta cggaggatt 
      caagaagaa tcggttaga attaatggg acgttcagt 
      atcagaggc cgcaaaacg tacaaaaac gtcctcaaa 
      gtgcacgaa cttgagctt aatcggctg aacgacgtt 
      acgatcagt gaagggaac gggaaaatt caaaatctc 
      gttgttaaa ggtggcctc aagtcggag aatttgacg 
      gtgcaaaca attaacggc gttaatcta actaattta 
      accgccaac actttatac ctcaacaag gcccaaaat 
      ctctccaaa cttaacttt gacaaaatt tcagttgcg 
      aatttgttt acgtccaca ttaaacaac aaaagtgta 
      gccgaactg gtcgagaaa gttgccaaa atcaccaac 
      atttccaaa attgagaac atggtcgtt actgaccat 
      gtcacaatc aaaaacgct gaaaacctg gcgacaatt 
      aacgacgtc aaaattgag caattaatg gcaaaaagt 
      cactcgagg taccacgac ggcacaatt tttggaaat 
      gtcaaattt tcaggcctt gtcaaaatt cgagatttg 
      caggtcgtt gtcataaat ggcgtcgat ttcgagaaa 
      ttgtcaaaa cgaatttta tacaagtcg tgggaacag 
      acaatcaac gaacattgt gtttttaac agactcatc 
      acagataac ttaataact gaccacata aacgaattt 
      gaagtggca aacctgatc gacgtttcg agcaccaaa 
      ccccagaaa atttttgct cgcggaggc ttaaaattc 
      gcgaaaaac gccacaatc aaaaatttg ttttcaaac 
      acgagttgt gacctaaac gagggtttg aagaactta 
      cgaaaccct ccctcacaa ctttgggac gagattaca 
      gtcaccgga aacgcattt ttctgggac agggaatca 
      cccattagc gacattttt gaaaatgtg gtcagcaat 
      tacagcaaa aatcacaca attaagggc aacgtgaat 
      tttgcgcac catgttttc gccaacaac ataacagct 
      ttgaaaaat gtcaataac gtgaacatt tcggaaatc 
      atcgacgac gcgtttgtg aacaaaccg aacttggag 
      caggttata accgggcaa aaaatcttc cagaagctg 
      gaagccgaa aacgtcgag attttgcaa aacacgaac 
      gtttcgtca atcaacaaa ctaaacatt tccgaaatc 
      gacgctaaa attatcaaa aaatcggcc aacgttgcg 
      gttaaaggc aacaaagtg tttttcggc ggcttggaa 
      acgaaaaaa attgagact gagtttata tcaggagtt 
      gaggcgaaa aatttggtc agttttgtg aaccccacc 
      caggtgccc aacgcaatg ttccgttca gtggctgtt 
      ttgagaaat ttcaatgtc aaatttatc aacaacatg 
      ccccttgat gtagttctc aaagaacga gttttgaaa 
      aaagggccc aaacaagtc ttgacaggg aactacttc 
      ctccaaaat gtccaattc aacggaaat ataacaacg 
      tctcgaata aatgatgtg gggatggaa aatgttgtt 
      tatgataac aaaaatcac gctattaat agtccgaag 
      aaatttaac cacgttgtg ataagtggg gacatggaa 
      gcggagctt gttaatggg ttcaagtta tcgcaattg 
      tacgaacag tctgttttg agtaatgag gagaccaag 
      tttggaaaa tcactgtat gtcaataaa ccaagttcc 
      atgagtggc aacattcaa acaaactac ataaacaac 
      atttcggtc aattacatg ctggacacg agtaaagaa 
      aattctcta aattcaaac actaaacaa atacgtgaa 
      ataacaacc attgtgaca aactttatc aaaacgtac 
      tttaattcg acgatgagg atgccaact gaagtaatg 
      tatttggaa aaatcaaaa gatttggac atttccctc 
      ccgaatgta attggagcg gaagttgtg gaaaccaaa 
      aatgatctt ctaattcac atcgagagt gcagaagag 
      ggtccctct tgtggttta cccccaact tgcaaatgc 
      cccacccag caaacaatg caagtttca agcaaacac 
      tctgtaaat atgttcctg aataaggcg acccagcga 
      atatacagt cactatgac gaagacatt gttgtgcat 
      tttatcacc cacagcatc agcactgat tcctactgc 
      agtgcaaat aaggacaag gtcctggac gaagtttcg 
      tccctgact tggactacc acaccccgt ggcaacaat 
      acggggggt ttcttcatt catcgcggc ttcttcaca 
      ggatatata accggagtc aagtttttc acaatttat 
      ggcaaaacc tacatcttt gttggaatt tcttacgac 
      ccgatttcg gaaacacac gccgttgac agtttagta 
      ttgcggttt aatgaagat cggacgaaa attgaacaa 
      attcagaaa ataccaacc gttggtttg aaaactatt 
      tcattgttt ccgacagca caaggggtg gtgctagtc 
      ctaggggtt gatgttaat aatacgaat acacaaatt 
      ttccggttt aatcaccac aaagaacag ttcgagctt 
      ctgcgcgag ttacccact tttgactgc gttttcgct 
      caaggggtt gtgctcgaa atggacagt ttgattgtc 
      ttggccaat cagcgcgcg gatttgcac attttgaaa 
      tatcagccg gagtttgat agttactat ttctatcaa 
      agcttcgag ttggattat ccggtctcc tcgctctct 
      gtatattac gctggaggt tttgggatt agcgatgcc 
      tatttaagc gtcgtgacg caaaacaac aaatatttc 
      atcttttcg ttccagttt atcgcaggt tggaagctc 
      gaatcaagc ggtcagttg gacggagtc aaggttttg 
      gtaccgttc cgaatggac aaaaaatcg taccttttg 
      gctccgtct aaaacaagt tcaatgctg tccgttgta 
      gaacacggc ttggattaa tttcgtgtc ttgttattt 
      ttattgttg attgttgta attctaatg taaaaaata 
      aatgaaaca aaaagcg