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Gene information
General information
TC011540 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid14: 2605640..2611034
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011540
    iBeetle screen iB_02951
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011540 RA
      MKVGSFTFL AIWLYLTAI YVSSRKSDS GIWYWQCNT 
      DEETCTRIS STVSRNTDT YPTLETCRL VCGKYGALW 
      PQPTSVTKI SSTLLKFPY RSIKFNIPD EKNEVNDFL 
      REISWIFLE TVANENTTN CPEQKNTVT VTFTVQSDD 
      TTLNWGTNE SYNLDLTTT GNQIGVQIS APTIFGARH 
      GLETLSQLM DVYPNNDGT KCLVVTDEA SISDAPFFP 
      HRGLLLDTA RNFLTVSKI KKHIDGMAA SKLNVLHWH 
      ITDSQSFPL ELPQLPNMT KFGAYSSDK IYHPEDITN 
      LLGYAKLRG VRIIIEIDA PSHAGNGWQ WGPDAGLGN 
      LSVCIDQQP WRSYCIQPP CGQLNPINP NVFDVLKLL 
      YNDIVNMLP KGEIFHMGG DEVYIPCWN ATPEIITYL 
      EKNGKPRTT DTFLDLWSD YQNKSLAAF DFVARNSDT 
      PIILWTSHL TQADVIEKY LSKARYVIQ TWVPASDNL 
      PTLLLELGY RIIVSTKDA WYLDHGFWG TTEYHNWRV 
      VYNNKIPTG DGALGGEVC MWGEYVDDS SVESRVWPR 
      AAAAAERLW TNPSDYVKQ TERRFYRHR ERLVARGIH 
      AEALVPRWC YQNEGECW
    CDS sequence
    • >TC011540 RA
      atgaaggta ggttccttt accttcctg gcaatatgg 
      ctctattta acagcaatt tacgtttca tctcgaaaa 
      tcagatagc gggatttgg tactggcaa tgtaacaca 
      gacgaggaa acctgcaca agaatttca agcactgtg 
      agcagaaac acagatacg tatcctact ttagaaaca 
      tgtagactg gtttgtggc aaatatggg gcgctatgg 
      ccccagcca acaagtgtt actaaaatt agtagcacc 
      ttattgaag tttccttat cgttctata aagtttaat 
      atcccggat gagaaaaac gaagttaac gattttctc 
      cgcgaaata tcttggatt ttccttgaa acagttgca 
      aatgagaat acgacaaac tgtcccgaa caaaaaaat 
      acagtaaca gtcactttc acagtacaa tcggatgat 
      acaaccctt aattgggga acgaatgag agttataat 
      ctagaccta acaacaaca ggaaatcaa attggggtt 
      caaatatct gctcctaca atttttgga gcgagacac 
      ggtttggaa acattgtca cagttgatg gatgtttac 
      ccaaataat gatgggacg aaatgtttg gtggtaaca 
      gacgaagcg tcaatttct gacgcccct ttttttcca 
      catagagga ttgttgctc gatactgcc agaaatttc 
      ttaacagtt tccaagata aagaaacat atcgatgga 
      atggccgct tcgaaacta aacgtcctc cactggcac 
      ataactgac tcgcaaagt tttcccttg gagttgcct 
      cagcttcca aacatgaca aaattcggc gcttattcg 
      agtgataaa atttaccac cctgaagat attaccaat 
      cttctaggc tatgctaaa ttgcgagga gttaggatt 
      ataatagag attgacgcc ccctcccat gctggtaat 
      ggttggcag tggggcccc gatgcaggt cttgggaac 
      ctgtccgtt tgtatcgac caacagccc tggaggtcg 
      tactgtatc cagccccct tgtggtcag ttgaatcca 
      attaacccc aacgttttt gacgtttta aagcttttg 
      tacaacgat attgttaat atgcttcca aagggggaa 
      atttttcac atggggggt gatgaggtt tacattccg 
      tgttggaat gcaacgcct gaaattatt acttatctc 
      gagaaaaat gggaaacct aggaccaca gacacattt 
      ctggatttg tggtccgat taccaaaac aaatcgctt 
      gctgcgttt gatttcgtg gcacggaat agtgacaca 
      cctatcatt ctatggacg agtcatttg acccaagct 
      gatgttata gaaaaatat ctttcaaag gccagatac 
      gttatacaa acctgggtt cccgcaagt gacaatttg 
      ccaaccttg ctcctagaa ttaggatac cggataatt 
      gtgtcaact aaggatgca tggtattta gaccatggg 
      ttttggggt actactgaa tatcacaac tggagggtt 
      gtttacaat aataaaatt ccgactgga gatggggct 
      ctgggtgga gaggtgtgt atgtggggt gaatatgtg 
      gatgacagt tccgtggag tcaagagta tggccaagg 
      gctgcagct gcagctgaa cgactttgg accaatcct 
      agcgattac gtaaaacag accgaacga aggttttat 
      agacacagg gaacgactg gtagccaga ggcattcat 
      gctgaagct ctggtccca aggtggtgt taccaaaat 
      gagggagaa tgttggtaa
    mRNA sequence
    • >TC011540 RA
      gattatcac caacaaact cattgtaaa attcattgt 
      tgtgggtgt taaagtagg aagcaaatg accctccag 
      tccaattaa caaaatgta acgttaggt atttgatat 
      tctcgcagt gtaaatccg tactgattt cataaaata 
      atcccgcat ttaaataat gttataaaa agagcgttt 
      tctgaatac taatgaaca caattaacc gtcacttga 
      atttcctct tctgatatg aactgcgtg cgcagtaac 
      ttagatgat agcactttg attatgttt ctcacattt 
      ttaagattg gagaattgt gttattcac agaaggtat 
      tcccacaca taaaaatca acatgaagg taggttcct 
      ttaccttcc tggcaatat ggctctatt taacagcaa 
      tttacgttt catctcgaa aatcagata gcgggattt 
      ggtactggc aatgtaaca cagacgagg aaacctgca 
      caagaattt caagcactg tgagcagaa acacagata 
      cgtatccta ctttagaaa catgtagac tggtttgtg 
      gcaaatatg gggcgctat ggccccagc caacaagtg 
      ttactaaaa ttagtagca ccttattga agtttcctt 
      atcgttcta taaagttta atatcccgg atgagaaaa 
      acgaagtta acgattttc tccgcgaaa tatcttgga 
      ttttccttg aaacagttg caaatgaga atacgacaa 
      actgtcccg aacaaaaaa atacagtaa cagtcactt 
      tcacagtac aatcggatg atacaaccc ttaattggg 
      gaacgaatg agagttata atctagacc taacaacaa 
      caggaaatc aaattgggg ttcaaatat ctgctccta 
      caatttttg gagcgagac acggtttgg aaacattgt 
      cacagttga tggatgttt acccaaata atgatggga 
      cgaaatgtt tggtggtaa cagacgaag cgtcaattt 
      ctgacgccc ctttttttc cacatagag gattgttgc 
      tcgatactg ccagaaatt tcttaacag tttccaaga 
      taaagaaac atatcgatg gaatggccg cttcgaaac 
      taaacgtcc tccactggc acataactg actcgcaaa 
      gttttccct tggagttgc ctcagcttc caaacatga 
      caaaattcg gcgcttatt cgagtgata aaatttacc 
      accctgaag atattacca atcttctag gctatgcta 
      aattgcgag gagttagga ttataatag agattgacg 
      ccccctccc atgctggta atggttggc agtggggcc 
      ccgatgcag gtcttggga acctgtccg tttgtatcg 
      accaacagc cctggaggt cgtactgta tccagcccc 
      cttgtggtc agttgaatc caattaacc ccaacgttt 
      ttgacgttt taaagcttt tgtacaacg atattgtta 
      atatgcttc caaaggggg aaatttttc acatggggg 
      gtgatgagg tttacattc cgtgttgga atgcaacgc 
      ctgaaatta ttacttatc tcgagaaaa atgggaaac 
      ctaggacca cagacacat ttctggatt tgtggtccg 
      attaccaaa acaaatcgc ttgctgcgt ttgatttcg 
      tggcacgga atagtgaca cacctatca ttctatgga 
      cgagtcatt tgacccaag ctgatgtta tagaaaaat 
      atctttcaa aggccagat acgttatac aaacctggg 
      ttcccgcaa gtgacaatt tgccaacct tgctcctag 
      aattaggat accggataa ttgtgtcaa ctaaggatg 
      catggtatt tagaccatg ggttttggg gtactactg 
      aatatcaca actggaggg ttgtttaca ataataaaa 
      ttccgactg gagatgggg ctctgggtg gagaggtgt 
      gtatgtggg gtgaatatg tggatgaca gttccgtgg 
      agtcaagag tatggccaa gggctgcag ctgcagctg 
      aacgacttt ggaccaatc ctagcgatt acgtaaaac 
      agaccgaac gaaggtttt atagacaca gggaacgac 
      tggtagcca gaggcattc atgctgaag ctctggtcc 
      caaggtggt gttaccaaa atgagggag aatgttggt 
      aatggaaac attttttta atacaattc at