Status
Please wait ...
Gene information
General information
TC030644 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4245511..4248855
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030644
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02948

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030644 RA
      MASNTYRVE WVEIIEPKT GEHMYANLA TGECVWDPP 
      EGVPVKRTD SNQWWELFD QNTARFYYY NATSQRTVW 
      HKPTNCDII PLAKLQTLK HNTDCSPGA AHAPTTNQA 
      TQTGEATGR GQVLSLSAS TPQLRRKSS DLCRSSSFS 
      QRGAEAPLY SNWHDSHLL PLEHFLLNN SRDSDSDHS 
      DSGSESLTG HEPDNEDSD QSEGSYLPH RFLHRPVEY 
      LNLPTAPER EADMEKFAA DNLNLGGRG LFRRKASVR 
      DLLSWSARS LQRPLLASS KPVARQALD MFRQVQMYM 
      GDRKARPGV SMNSLLMDI LGTAHTQSL LRDELFVQL 
      CRQTTENPR RDSLLRGWE LLTIALAFV PPSPLFQPA 
      LLGYLNRHR DPGFSRVFP DVSRWPIHV QVNHYATVA 
      CQRLERIGA GGKRSARRP QMDDIDSAR KQIFQASLF 
      GNTLREVMQ LQASRWPTR RLPWPQVEL SQQVLRLQG 
      LSTEGIFRV SADVDEVNR LKAQMDKWE LSEPSDAHV 
      PANLLKLWL RELYEPLIP DSLYPECVA EPMTSRRAC 
      DLVLRLPTL HRLVLCYLI RFLQIFNKP DVIQHTKMD 
      ASNLAMVFA PNCLRCMAS DPRTMFDNA RKEMAFMRC 
      LIQDLDTEC VRDMI
    CDS sequence
    • >TC030644 RA
      atggcttct aatacttat agggtagaa tgggtagaa 
      ataatagag ccgaaaaca ggcgagcat atgtatgcg 
      aatttagca acaggggag tgtgtctgg gaccctcct 
      gagggtgtt ccggtcaaa agaaccgac tctaaccag 
      tggtgggag ctcttcgat caaaacacg gcccggttc 
      tactactac aatgccact tcccagcgc accgtgtgg 
      cacaaaccc acaaattgt gacataata cctctagcc 
      aaattacaa actttgaaa cacaacacc gactgcagt 
      cccggggct gctcacgcg cccaccacc aatcaggcc 
      acgcaaacc ggggaggcc acaggccgc ggccaagtg 
      ctaagttta tcagcgagt acgccacaa ctgcggcgg 
      aagtcgagt gatttgtgc cgaagtagc agttttagt 
      cagagaggt gccgaagca cctttgtac tccaactgg 
      cacgattcg catttattg cctttggag cacttcctt 
      ctgaacaac agccgcgat tcggatagt gatcattcc 
      gacagcgga agtgagtct ctaactggc catgagccc 
      gataacgag gattcggat cagtcggaa ggcagctac 
      ttgcctcac cggttcctc cacagacca gtcgagtac 
      ctcaattta ccaacggcc cctgaacgt gaggcggac 
      atggagaag tttgcggct gataatttg aatcttggg 
      ggcaggggg ctgttcagg aggaaagca agtgttcgc 
      gatttgtta agctggtcg gctaggagt ctgcaaagg 
      cctctctta gcctcaagt aaaccagtt gcgagacaa 
      gctttagat atgtttaga caagtacaa atgtatatg 
      ggcgaccgc aaagctaga cctggagtt tctatgaat 
      tcgttactc atggatatc ctggggaca gcacatacg 
      cagtctctt ctaagagat gaactgttc gttcagttg 
      tgtcggcag acgactgaa aacccgagg cgcgattcc 
      cttttgcgt ggatgggaa ctacttacc attgcttta 
      gcttttgtt cctcccagt cccttgttc caaccagct 
      ctcttaggc tatctcaat cgacataga gatccgggg 
      ttttcgaga gtttttccg gacgttagt agatggcca 
      atacacgta caggtgaat cattacgca actgtggct 
      tgccagcgg ttggagcga attggcgcg ggtgggaag 
      aggtccgcg cgtcggccc caaatggac gatatagat 
      tcagcccgc aaacaaata tttcaagct agtcttttt 
      ggtaatact ctacgcgaa gtgatgcag cttcaggcc 
      agtcgttgg ccgactcgt cggctgccc tggcctcaa 
      gtggagttg tctcagcaa gtcctccga ttacaagga 
      ttgagcacg gagggcata ttccgcgtg tccgcagac 
      gtggacgaa gtgaaccga ttgaaggcc cagatggac 
      aaatgggaa cttagcgaa ccaagtgat gctcatgtc 
      ccagcgaat ttgcttaag ttgtggttg cgtgaattg 
      tatgaacct ttaataccc gactctctt tatcccgaa 
      tgcgtcgct gaacccatg acctctcgc cgtgcatgt 
      gatctagtt ctccgttta ccgactttg cacagactt 
      gttttatgc tatttaatt cggtttttg caaattttt 
      aataaacct gatgttatc cagcatact aaaatggat 
      gcttccaat ttggctatg gtctttgcg cctaactgc 
      ttgcgttgt atggcgagc gatccgcgt accatgttc 
      gacaatgct cgcaaagaa atggccttt atgcggtgt 
      ctcattcaa gatcttgac accgaatgt gttcgcgat 
      atgatctga
    mRNA sequence
    • >TC030644 RA
      agaaatata caaacaata atggcgtcc gcaatttat 
      attactgaa agtggcctt ttttccttc tggttctgg 
      tactggccc acttttaag ccgtaaatt tccatgctg 
      gtccccgct gctctgttt gttgaagca ccaaagact 
      ttgtcttcg tcgtgggat tgttaacct ataagaaaa 
      tgacgtttg acatatcat aatagtagt agtgttttg 
      ttagaaaat ggcttctaa tacttatag ggtagaatg 
      ggtagaaat aatagagcc gaaaacagg cgagcatat 
      gtatgcgaa tttagcaac aggggagtg tgtctggga 
      ccctcctga gggtgttcc ggtcaaaag aaccgactc 
      taaccagtg gtgggagct cttcgatca aaacacggc 
      ccggttcta ctactacaa tgccacttc ccagcgcac 
      cgtgtggca caaacccac aaattgtga cataatacc 
      tctagccaa attacaaac tttgaaaca caacaccga 
      ctgcagtcc cggggctgc tcacgcgcc caccaccaa 
      tcaggccac gcaaaccgg ggaggccac aggccgcgg 
      ccaagtgct aagtttatc agcgagtac gccacaact 
      gcggcggaa gtcgagtga tttgtgccg aagtagcag 
      ttttagtca gagaggtgc cgaagcacc tttgtactc 
      caactggca cgattcgca tttattgcc tttggagca 
      cttccttct gaacaacag ccgcgattc ggatagtga 
      tcattccga cagcggaag tgagtctct aactggcca 
      tgagcccga taacgagga ttcggatca gtcggaagg 
      cagctactt gcctcaccg gttcctcca cagaccagt 
      cgagtacct caatttacc aacggcccc tgaacgtga 
      ggcggacat ggagaagtt tgcggctga taatttgaa 
      tcttggggg cagggggct gttcaggag gaaagcaag 
      tgttcgcga tttgttaag ctggtcggc taggagtct 
      gcaaaggcc tctcttagc ctcaagtaa accagttgc 
      gagacaagc tttagatat gtttagaca agtacaaat 
      gtatatggg cgaccgcaa agctagacc tggagtttc 
      tatgaattc gttactcat ggatatcct ggggacagc 
      acatacgca gtctcttct aagagatga actgttcgt 
      tcagttgtg tcggcagac gactgaaaa cccgaggcg 
      cgattccct tttgcgtgg atgggaact acttaccat 
      tgctttagc ttttgttcc tcccagtcc cttgttcca 
      accagctct cttaggcta tctcaatcg acatagaga 
      tccggggtt ttcgagagt ttttccgga cgttagtag 
      atggccaat acacgtaca ggtgaatca ttacgcaac 
      tgtggcttg ccagcggtt ggagcgaat tggcgcggg 
      tgggaagag gtccgcgcg tcggcccca aatggacga 
      tatagattc agcccgcaa acaaatatt tcaagctag 
      tctttttgg taatactct acgcgaagt gatgcagct 
      tcaggccag tcgttggcc gactcgtcg gctgccctg 
      gcctcaagt ggagttgtc tcagcaagt cctccgatt 
      acaaggatt gagcacgga gggcatatt ccgcgtgtc 
      cgcagacgt ggacgaagt gaaccgatt gaaggccca 
      gatggacaa atgggaact tagcgaacc aagtgatgc 
      tcatgtccc agcgaattt gcttaagtt gtggttgcg 
      tgaattgta tgaaccttt aatacccga ctctcttta 
      tcccgaatg cgtcgctga acccatgac ctctcgccg 
      tgcatgtga tctagttct ccgtttacc gactttgca 
      cagacttgt tttatgcta tttaattcg gtttttgca 
      aatttttaa taaacctga tgttatcca gcatactaa 
      aatggatgc ttccaattt ggctatggt ctttgcgcc 
      taactgctt gcgttgtat ggcgagcga tccgcgtac 
      catgttcga caatgctcg caaagaaat ggcctttat 
      gcggtgtct cattcaaga tcttgacac cgaatgtgt 
      tcgcgatat gatctgacc tcagtttat acattttta 
      caaatgtac ttcttaatg catcatttt tgtattata 
      accatagac attactatt ttatttcta ttattgtcc 
      ctgtattga aatttgatt gtgatttaa attatatta 
      ttgaaataa atttgtttt tttaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY