| General information | |||
| TC004640 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 4264674..4272731 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC004640 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02947 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC004640 RA
MTSGTSSPT PTDSPSLSQ RPLLTAITP IPKKTVEFE TMPAKRSDA AAKDPESLT LRQKRGRLT QPRMSLLGR PLNYRASRR DARYRRIQS RVYNFLERP RGVAIFYHM IVFLMVFTC LTLSVFSTI ETYEDMTVQ VLTYMESFV VIWFAIEFS LRLWSSGCR SRYQGYIGR LKFLRRPFC IIDVITIVA SIVVLVMRS RGQVLATSA LRGLRFFQI LRMVRMDRR GGTWKLLGS VVYAHRQEL ITTLYIGFL GLIFASFLV FLAEKDVNK NFSNFAQAL WWGVITLCT VGYGDMVPE TWQGKIIAS FCALLGISF FALPAGILG SGFALKVQQ QQRQKHMIR RRQPAAMLI QSLWRCYAA DEHSLSVAT WKIHQVPLP SPPSRVSSS FKHNASFVS RLPTIRRHK STSLHSPSM HKGPPRSGR SFVDLNASA ENLGGVSNN GSRNLSHSE DSVAETMLS KKNSDDEDD EPRYIQLTN QHKGAIRAI RKLKYFVAR KKFREALKP YDVKDVIEQ YSSGHADLL NRVKNLQFR LDQILGKQG SKAKDVYAS KISLASRVV KVERQVDDI ESKIDQLLE LYMEDRKRF LSLPYIQPS AEKNTTSSS TTVNLCSSN MTNPSSTIL KPKPILVEK QSSEPSSPI TKSFKEHST AAVTKRPMH RGFSDLGNR IKKRVTLSS ISSNYVCPP DGMSYDGEV VIVVPPINN TPSTVGASA QEPDLGSPK TATESSGVK KLTRNRNCE EDEDDAAEK PLLCRSKST EILITPTTL ETTREEFYD NKTNQQSKN KFLKDDV
- >TC004640 RA
atgacatct gggacttct tcgccaaca ccaacggat tcgccgtcc ttatcacaa cgaccttta ctcactgca ataactcca ataccgaag aaaactgtt gaatttgaa accatgcca gcgaaacgg tccgatgct gcagccaag gatccggaa agtttgacg ttgcggcaa aaacgcggc cgtctcaca caaccccgg atgagtctt cttggtaga ccgctcaat tatagagct tcacgccga gatgctcgc tatcgtcga attcaaagt cgggtttat aacttccta gagagacct cggggggtc gctatcttc taccacatg atagtcttc ttgatggtt tttacatgt ttaactttg agtgtgttt tcaacgatt gagacttac gaagacatg actgttcaa gtcctaact tacatggaa tcgttcgtc gttatttgg tttgctatt gaattcagc ctaaggctt tggtcttcg ggatgtcgc tcccgctac caaggctac atcggccgc cttaaattt cttagacgt ccgttctgt attattgat gttataaca attgtggct tccattgtt gttctggtt atgaggtct cgcggtcaa gttttagcg actagcgcc cttcgtggt ttacgattt ttccaaatt ttgcggatg gtccgaatg gacagacgt ggagggact tggaaactg ttgggctca gtcgtgtac gcacatcga caagaactt ataactacg ttatacata ggatttttg ggcttgatt ttcgcttca tttttggtc ttcctggcg gaaaaagac gtgaacaaa aatttctcc aattttgca caagctttg tggtggggt gttattaca ttgtgcact gttggctat ggggatatg gttccggaa acatggcaa ggcaaaatc attgcttcg ttttgtgct ttacttggt atatcgttt tttgcattg ccggctgga attttagga tccggtttt gctttaaaa gttcagcaa caacaaagg caaaagcat atgatcagg aggagacaa ccagccgca atgctaatt cagtcgctt tggcgatgt tacgcagcc gatgagcac tccctatct gtggctacg tggaaaata catcaagtt cccttaccc agcccgccc tcacgagtt tcgagcagt tttaagcac aatgcatct tttgtaagt cgattaccg acaattcgt cgccacaaa agcacttca cttcattcc ccgtcgatg cataaagga ccaccaaga agtggtagg agtttcgtg gatttaaac gcatcagca gaaaattta ggcggagtc agcaacaac ggatcgcgg aatttgagc catagcgag gattcggtt gcagagacg atgctttcg aaaaaaaat tccgacgat gaggacgac gagccgaga tatatacag ttaacgaat cagcacaaa ggggcaatc cgggccatc cgtaagctc aagtatttt gtggcacga aagaagttt agagaggcc ttgaagcca tatgacgtt aaagacgtt atagaacaa tattcgtct gggcatgca gatcttttg aatagagtg aaaaactta cagttcaga ttagatcaa atcctaggg aaacaaggc tccaaggcg aaagatgtt tatgcttcc aaaatcagt ttagcatcg cgcgtagtc aaagttgaa agacaagta gatgacata gagtctaag attgaccag cttttagag ctttacatg gaagaccgt aaacgcttc ctctctctt ccttacatc cagccaagc gccgaaaaa aacaccact tcctctagt acaaccgtc aatctatgt tcttcaaac atgacaaat ccgtcgagt acaatcctt aaacctaaa ccaatactt gtagagaaa caatcatcc gaaccaagt tcgccaatc acaaaaagt tttaaagaa cattcaacg gccgcagta acaaaacga ccaatgcat cgcggattt tccgacctc ggcaatcgg attaaaaaa agagtgaca ttaagttca atttcgtcg aattatgtg tgcccaccg gatggcatg tcctacgac ggcgaagtc gtgattgtg gttcccccc atcaacaac acgccatcg acagttggt gcttcggca caggagccg gacttaggg agcccaaag acagccaca gaaagctct ggggtgaaa aagttaacg agaaatcga aattgtgaa gaagatgaa gacgacgca gcggagaag ccgttacta tgccgttcg aaatcaaca gaaatactc atcacgcca acgacttta gaaaccacc agagaagaa ttttatgat aataaaact aatcaacaa tcaaaaaac aaattttta aaagacgat gtatga
- >TC004640 RA
ccgtccgtt tttcgggtt aatccgttt atatctcca attctattg atttattaa ctccttgat gatgccgga gatgccgaa aatttttca ctctttcga tattgccgc tcccccaca acaccctca tgaatactt aaatgccgc tttgagagg agagtaaca cgcttgagg caaaatgac atctgggac ttcttcgcc aacaccaac ggattcgcc gtccttatc acaacgacc tttactcac tgcaataac tccaatacc gaagaaaac tgttgaatt tgaaaccat gccagcgaa acggtccga tgctgcagc caaggatcc ggaaagttt gacgttgcg gcaaaaacg cggccgtct cacacaacc ccggatgag tcttcttgg tagaccgct caattatag agcttcacg ccgagatgc tcgctatcg tcgaattca aagtcgggt ttataactt cctagagag acctcgggg ggtcgctat cttctacca catgatagt cttcttgat ggtttttac atgtttaac tttgagtgt gttttcaac gattgagac ttacgaaga catgactgt tcaagtcct aacttacat ggaatcgtt cgtcgttat ttggtttgc tattgaatt cagcctaag gctttggtc ttcgggatg tcgctcccg ctaccaagg ctacatcgg ccgccttaa atttcttag acgtccgtt ctgtattat tgatgttat aacaattgt ggcttccat tgttgttct ggttatgag gtctcgcgg tcaagtttt agcgactag cgcccttcg tggtttacg atttttcca aattttgcg gatggtccg aatggacag acgtggagg gacttggaa actgttggg ctcagtcgt gtacgcaca tcgacaaga acttataac tacgttata cataggatt tttgggctt gattttcgc ttcattttt ggtcttcct ggcggaaaa agacgtgaa caaaaattt ctccaattt tgcacaagc tttgtggtg gggtgttat tacattgtg cactgttgg ctatgggga tatggttcc ggaaacatg gcaaggcaa aatcattgc ttcgttttg tgctttact tggtatatc gttttttgc attgccggc tggaatttt aggatccgg ttttgcttt aaaagttca gcaacaaca aaggcaaaa gcatatgat caggaggag acaaccagc cgcaatgct aattcagtc gctttggcg atgttacgc agccgatga gcactccct atctgtggc tacgtggaa aatacatca agttccctt acccagccc gccctcacg agtttcgag cagttttaa gcacaatgc atcttttgt aagtcgatt accgacaat tcgtcgcca caaaagcac ttcacttca ttccccgtc gatgcataa aggaccacc aagaagtgg taggagttt cgtggattt aaacgcatc agcagaaaa tttaggcgg agtcagcaa caacggatc gcggaattt gagccatag cgaggattc ggttgcaga gacgatgct ttcgaaaaa aaattccga cgatgagga cgacgagcc gagatatat acagttaac gaatcagca caaaggggc aatccgggc catccgtaa gctcaagta ttttgtggc acgaaagaa gtttagaga ggccttgaa gccatatga cgttaaaga cgttataga acaatattc gtctgggca tgcagatct tttgaatag agtgaaaaa cttacagtt cagattaga tcaaatcct agggaaaca aggctccaa ggcgaaaga tgtttatgc ttccaaaat cagtttagc atcgcgcgt agtcaaagt tgaaagaca agtagatga catagagtc taagattga ccagctttt agagcttta catggaaga ccgtaaacg cttcctctc tcttcctta catccagcc aagcgccga aaaaaacac cacttcctc tagtacaac cgtcaatct atgttcttc aaacatgac aaatccgtc gagtacaat ccttaaacc taaaccaat acttgtaga gaaacaatc atccgaacc aagttcgcc aatcacaaa aagttttaa agaacattc aacggccgc agtaacaaa acgaccaat gcatcgcgg attttccga cctcggcaa tcggattaa aaaaagagt gacattaag ttcaatttc gtcgaatta tgtgtgccc accggatgg catgtccta cgacggcga agtcgtgat tgtggttcc ccccatcaa caacacgcc atcgacagt tggtgcttc ggcacagga gccggactt agggagccc aaagacagc cacagaaag ctctggggt gaaaaagtt aacgagaaa tcgaaattg tgaagaaga tgaagacga cgcagcgga gaagccgtt actatgccg ttcgaaatc aacagaaat actcatcac gccaacgac tttagaaac caccagaga agaatttta tgataataa aactaatca acaatcaaa aaacaaatt tttaaaaga cgatgtatg acataattt aaatttgtt attagttta tatattatg accgttaaa tattcaaaa taatgtaaa tgattatag atttttgga cctaaaact tgcgaatat ttttaacat gtactgaac ttagggcac aataatgac gcttattaa tataaaaat atatttgaa aagtttgta cttcttcac ttaggatta aaatttgct gtagcatgt tttagctgt gatttgttt cgacgtttt atactgaaa ctttacagg gacattggc caatgcttg tagattaat tcagttacg tttcacaag tgaatttta acggcaact tgtacttac aacgaccac actataatc ctaacgttt ttataatat caagtagaa aaaaacgcc taacaaaca atcaacgac tttaaaaat gtgctgctg aaattcact tttaatcag tagactata ctataaaat ttagctatt taaaaatgt acgtaaaat aatgtgatt aatttatag atacgacag taatatttt ttgacccga cccattgcc tactgttgt tacaataag tatttgttt gcttaaaaa tgatgtgga acatctata ctgtacggg atttttaat aaaac
