General information | |||
TC004717 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 4506885..4513893 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC004717 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02946 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
vitellogenic egg chamber late number decreased - Number of animals showing the phenotype: 4
tropharium potentiallystructure irregular - Number of animals showing the phenotype: 4
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC004717 RA
MERSRDYSP RGRSMSISP EYKGSPDYR SRSPSRSPH YKRHNGHRS PTFHSSKKF HHEYSDERG KNGSDYESD REYRRESSW DGDRRSRSR SPDYSRDRD RSKHYRSRD RDRDHHRSR GKRSRSGSR WERRSEDRK TRDRDRSYR DDDRDSVDS ERSPNGAVI GASGSEIIG YKSQPPNNT IMIRGLAQH ISENDIRQD IIHCGLMPK DIRLIRKKD TGTSRGFAF VEFSTLGEA IRWMDMKQG VLMLQEQYR AIMQYSIPK DVCANSEKP PSHKASADW FCIKCGAQN FKRRDNCFK CHASRMESE EGGSGSDEI CTYTTKTIM IRNLDALTT EDSVMSVLN TVIPDLVKS ISAVCIGRD PLTSTSRGI CYLGTESTI DALAIYGAL SNLSSPLTI DGKTVILSY CKYNMGDNK KAYSQADNV AFPNASVPS TYAMTDVES LAEYAARRY AKTPSEYMH YIKYYRNYF TQQISAGNS ITLHQENQM DAANAAAAV AQSAIQQMH ANKNFYDTT HMSIPNGTD GKRYPVPDV SKYTYDKTT GYQYDASTG LYYDPNSNY YWNSVIQKY LYWDQEKMT YVLAPMTYE SYTGQQTMA ASTGSQEPE AKKPKAEKQ DKVKVAKKI AKDMERWAK TLNQKKEMS FARANSDMG ASSSGSASA DVGFSVLEK KNTSQLVPG FYKNDSDES IPAATAPLV AAYGGESDS SGEEEIDEI ELLDYAKLI CNLCKRQLG SAEALTKHA KLSALHKQN LEARKKTKK LEGTKEKVV YRDRAKERR MKYGDPDEP QPSKLKEKY LKSRELAEA PVAAASVSE PIGAENVGN RLLQKMGWT EGQGLGKQN QGRTTIIQA EQHTSTVGL GNKVAGYTA LAGESYKDC VKKMMYARY QELTEKEN
- >TC004717 RA
atggaaaga tctagagat tattcacct agaggacga agtatgtcc atatcacca gagtacaag ggttccccc gattataga agcaggtcg ccgtcgcgg tccccgcac tacaaaaga cacaacggg cacagatca cccacgttt cacagctca aaaaaattt catcatgaa tattccgac gaaagagga aaaaatggt agtgattat gagagtgac cgcgaatac cgaagagag tcttcttgg gatggtgac cggaggagt cgttcacga tctccggac tattcaaga gatcgggat agaagcaaa cattataga agtagggac agggatagg gaccaccat aggtctaga gggaaaagg tcgcgaagt ggttcacgg tgggaacga agatcagaa gatagaaaa acacgtgat cgagacagg tcatatcgc gatgatgat agggacagt gtggatagt gaacgtagc cctaacgga gctgttatt ggagctagc gggtctgaa ataataggc tacaaaagt cagcccccc aacaacacc atcatgatt cgcggactg gcgcagcac atctccgaa aacgatatt cgtcaggat attattcac tgtggtttg atgccgaag gacattcgt ctcatcaga aaaaaagac acaggtact tcacgtggt ttcgccttt gtcgagttt tcaacactt ggtgaggcc attcggtgg atggacatg aaacagggt gttttaatg ctgcaagaa caatatcgt gctataatg cagtatagc attcccaaa gacgtctgc gcgaattca gaaaaacca ccaagtcat aaggcttca gccgactgg ttttgcata aaatgtggc gcccagaac ttcaaaaga agggacaat tgtttcaaa tgccacgct tcacgaatg gaaagcgaa gaaggtggt agcggaagc gacgaaata tgcacttac accaccaaa accatcatg atacgcaat ctagatgcc ctgacaacg gaggattct gttatgtca gttcttaat actgtcatt cctgattta gtcaagtca atatcagca gtttgcatt ggtcgtgac cctttgact tcaacctcc agaggcatt tgctacctt ggaacagaa agcacgata gatgctttg gccatttac ggagctcta agcaattta tccagcccc ctcactatt gacggaaaa acagtgatt ttgtcgtat tgtaagtac aacatgggt gacaacaag aaagcctat tctcaggct gataacgtt gcttttccc aacgcatcc gtcccgagc acttacgca atgaccgat gtggaaagt ctagcagaa tatgctgcg agacgttac gctaaaact ccgagcgaa tacatgcat tacattaag tattaccgc aactacttc acccaacaa atcagtgcg ggcaactcg attacactc catcaagaa aaccaaatg gatgcagca aacgctgca gctgctgtc gcccaatcc gccatccaa caaatgcat gctaataaa aatttctac gatactacc cacatgtca attccaaac ggaaccgat ggcaagaga tatcctgtt cctgacgtt tcaaaatat acttacgac aaaaccact gggtatcaa tatgacgca agtacgggt ctatattac gatccgaat tcaaactat tactggaat agcgtcatt caaaaatac ctctactgg gaccaagaa aaaatgacc tacgtgttg gcccccatg acttacgaa agttacacg gggcagcaa accatggcc gcgtcgacc ggctcacaa gaaccggaa gcgaaaaaa ccgaaagcg gaaaaacag gacaaagtc aaagtcgcc aagaagatc gccaaagac atggaaagg tgggcgaag acgctaaac cagaagaag gagatgtcc ttcgcgcgt gctaactcc gacatgggc gccagcagt agcggtagt gcgtcagcc gatgtcggt ttttccgtc ctggaaaag aaaaacacg agccaattg gttccaggt ttttataaa aatgactct gacgaatcc attcctgca gccaccgct cctctcgtt gcagcctac ggtggcgaa agcgattcc tccggagaa gaagaaatc gacgagatt gagctccta gattacgcg aaacttatc tgtaacctc tgcaaacgt caattaggg tccgctgaa gctttaact aaacacgcc aaactatcc gccttgcac aagcagaac ctggaagcg cgaaagaag acgaagaag ctcgaaggc actaaagaa aaggtcgtt tacagagac cgggctaag gagcgaaga atgaaatac ggtgatccg gacgagcca caacccagc aaactcaaa gaaaagtat ttgaagtcg agggaattg gctgaagcg ccggtcgcc gcagcgtcg gtttcggaa ccgatcggg gccgaaaat gtcggcaat agactacta caaaaaatg ggctggact gagggccaa ggtttaggg aaacaaaat caaggaaga accacaatt attcaggcg gaacagcac actagcacc gtagggcta ggaaacaaa gttgcagga tatacagct ctggcagga gaatcgtac aaagactgt gttaaaaaa atgatgtat gcacgttac caggagttg acagaaaag gaaaactaa
- >TC004717 RA
ggctggcgc tttgaatgg gcgtgggaa tttttccca ggctttttt tgccaactg atgtcgtaa attgcaatt tttgcgatt gatttttgt cgattattt tataaaatt aagggtcct ccagggggg acatgcgcc ccacgttgc gctctctag tgcgcgttt ggagaagtc cacttggtg ccgattgtt gttggagag agaagttcc gaggataaa ttgttggcg tccttgtct aggtagtca aagtggtcg tctacagag gatgataac aacgtgacg atgccagtt tttgagtgt tgacgcgtt ggatggatt ttaacaatt ttcagaact tatggttga aattatgaa ctgagagtt tccgacttg gagcactcc aaacgcgcc gaatgtgta cagaattgc ggtaaataa aaatggaaa gatctagag attattcac ctagaggac gaagtatgt ccatatcac cagagtaca agggttccc ccgattata gaagcaggt cgccgtcgc ggtccccgc actacaaaa gacacaacg ggcacagat cacccacgt ttcacagct caaaaaaat ttcatcatg aatattccg acgaaagag gaaaaaatg gtagtgatt atgagagtg accgcgaat accgaagag agtcttctt gggatggtg accggagga gtcgttcac gatctccgg actattcaa gagatcggg atagaagca aacattata gaagtaggg acagggata gggaccacc ataggtcta gagggaaaa ggtcgcgaa gtggttcac ggtgggaac gaagatcag aagatagaa aaacacgtg atcgagaca ggtcatatc gcgatgatg atagggaca gtgtggata gtgaacgta gccctaacg gagctgtta ttggagcta gcgggtctg aaataatag gctacaaaa gtcagcccc ccaacaaca ccatcatga ttcgcggac tggcgcagc acatctccg aaaacgata ttcgtcagg atattattc actgtggtt tgatgccga aggacattc gtctcatca gaaaaaaag acacaggta cttcacgtg gtttcgcct ttgtcgagt tttcaacac ttggtgagg ccattcggt ggatggaca tgaaacagg gtgttttaa tgctgcaag aacaatatc gtgctataa tgcagtata gcattccca aagacgtct gcgcgaatt cagaaaaac caccaagtc ataaggctt cagccgact ggttttgca taaaatgtg gcgcccaga acttcaaaa gaagggaca attgtttca aatgccacg cttcacgaa tggaaagcg aagaaggtg gtagcggaa gcgacgaaa tatgcactt acaccacca aaaccatca tgatacgca atctagatg ccctgacaa cggaggatt ctgttatgt cagttctta atactgtca ttcctgatt tagtcaagt caatatcag cagtttgca ttggtcgtg accctttga cttcaacct ccagaggca tttgctacc ttggaacag aaagcacga tagatgctt tggccattt acggagctc taagcaatt tatccagcc ccctcacta ttgacggaa aaacagtga ttttgtcgt attgtaagt acaacatgg gtgacaaca agaaagcct attctcagg ctgataacg ttgcttttc ccaacgcat ccgtcccga gcacttacg caatgaccg atgtggaaa gtctagcag aatatgctg cgagacgtt acgctaaaa ctccgagcg aatacatgc attacatta agtattacc gcaactact tcacccaac aaatcagtg cgggcaact cgattacac tccatcaag aaaaccaaa tggatgcag caaacgctg cagctgctg tcgcccaat ccgccatcc aacaaatgc atgctaata aaaatttct acgatacta cccacatgt caattccaa acggaaccg atggcaaga gatatcctg ttcctgacg tttcaaaat atacttacg acaaaacca ctgggtatc aatatgacg caagtacgg gtctatatt acgatccga attcaaact attactgga atagcgtca ttcaaaaat acctctact gggaccaag aaaaaatga cctacgtgt tggccccca tgacttacg aaagttaca cggggcagc aaaccatgg ccgcgtcga ccggctcac aagaaccgg aagcgaaaa aaccgaaag cggaaaaac aggacaaag tcaaagtcg ccaagaaga tcgccaaag acatggaaa ggtgggcga agacgctaa accagaaga aggagatgt ccttcgcgc gtgctaact ccgacatgg gcgccagca gtagcggta gtgcgtcag ccgatgtcg gtttttccg tcctggaaa agaaaaaca cgagccaat tggttccag gtttttata aaaatgact ctgacgaat ccattcctg cagccaccg ctcctctcg ttgcagcct acggtggcg aaagcgatt cctccggag aagaagaaa tcgacgaga ttgagctcc tagattacg cgaaactta tctgtaacc tctgcaaac gtcaattag ggtccgctg aagctttaa ctaaacacg ccaaactat ccgccttgc acaagcaga acctggaag cgcgaaaga agacgaaga agctcgaag gcactaaag aaaaggtcg tttacagag accgggcta aggagcgaa gaatgaaat acggtgatc cggacgagc cacaaccca gcaaactca aagaaaagt atttgaagt cgagggaat tggctgaag cgccggtcg ccgcagcgt cggtttcgg aaccgatcg gggccgaaa atgtcggca atagactac tacaaaaaa tgggctgga ctgagggcc aaggtttag ggaaacaaa atcaaggaa gaaccacaa ttattcagg cggaacagc acactagca ccgtagggc taggaaaca aagttgcag gatatacag ctctggcag gagaatcgt acaaagact gtgttaaaa aaatgatgt atgcacgtt accaggagt tgacagaaa aggaaaact aatcaggtt aatcagttg tttcactga cttttgtca agtgccaag cagtgttaa ttaattttt catccaaac atcatgtta ataaattag aagtaagtt a
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
