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Gene information
General information
TC004717 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4506885..4513893
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004717
    iBeetle screen iB_02946

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber early number decreased - Number of animals showing the phenotype: 4

    vitellogenic egg chamber late number decreased - Number of animals showing the phenotype: 4
    tropharium potentiallystructure irregular - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004717 RA
      MERSRDYSP RGRSMSISP EYKGSPDYR SRSPSRSPH 
      YKRHNGHRS PTFHSSKKF HHEYSDERG KNGSDYESD 
      REYRRESSW DGDRRSRSR SPDYSRDRD RSKHYRSRD 
      RDRDHHRSR GKRSRSGSR WERRSEDRK TRDRDRSYR 
      DDDRDSVDS ERSPNGAVI GASGSEIIG YKSQPPNNT 
      IMIRGLAQH ISENDIRQD IIHCGLMPK DIRLIRKKD 
      TGTSRGFAF VEFSTLGEA IRWMDMKQG VLMLQEQYR 
      AIMQYSIPK DVCANSEKP PSHKASADW FCIKCGAQN 
      FKRRDNCFK CHASRMESE EGGSGSDEI CTYTTKTIM 
      IRNLDALTT EDSVMSVLN TVIPDLVKS ISAVCIGRD 
      PLTSTSRGI CYLGTESTI DALAIYGAL SNLSSPLTI 
      DGKTVILSY CKYNMGDNK KAYSQADNV AFPNASVPS 
      TYAMTDVES LAEYAARRY AKTPSEYMH YIKYYRNYF 
      TQQISAGNS ITLHQENQM DAANAAAAV AQSAIQQMH 
      ANKNFYDTT HMSIPNGTD GKRYPVPDV SKYTYDKTT 
      GYQYDASTG LYYDPNSNY YWNSVIQKY LYWDQEKMT 
      YVLAPMTYE SYTGQQTMA ASTGSQEPE AKKPKAEKQ 
      DKVKVAKKI AKDMERWAK TLNQKKEMS FARANSDMG 
      ASSSGSASA DVGFSVLEK KNTSQLVPG FYKNDSDES 
      IPAATAPLV AAYGGESDS SGEEEIDEI ELLDYAKLI 
      CNLCKRQLG SAEALTKHA KLSALHKQN LEARKKTKK 
      LEGTKEKVV YRDRAKERR MKYGDPDEP QPSKLKEKY 
      LKSRELAEA PVAAASVSE PIGAENVGN RLLQKMGWT 
      EGQGLGKQN QGRTTIIQA EQHTSTVGL GNKVAGYTA 
      LAGESYKDC VKKMMYARY QELTEKEN
    CDS sequence
    • >TC004717 RA
      atggaaaga tctagagat tattcacct agaggacga 
      agtatgtcc atatcacca gagtacaag ggttccccc 
      gattataga agcaggtcg ccgtcgcgg tccccgcac 
      tacaaaaga cacaacggg cacagatca cccacgttt 
      cacagctca aaaaaattt catcatgaa tattccgac 
      gaaagagga aaaaatggt agtgattat gagagtgac 
      cgcgaatac cgaagagag tcttcttgg gatggtgac 
      cggaggagt cgttcacga tctccggac tattcaaga 
      gatcgggat agaagcaaa cattataga agtagggac 
      agggatagg gaccaccat aggtctaga gggaaaagg 
      tcgcgaagt ggttcacgg tgggaacga agatcagaa 
      gatagaaaa acacgtgat cgagacagg tcatatcgc 
      gatgatgat agggacagt gtggatagt gaacgtagc 
      cctaacgga gctgttatt ggagctagc gggtctgaa 
      ataataggc tacaaaagt cagcccccc aacaacacc 
      atcatgatt cgcggactg gcgcagcac atctccgaa 
      aacgatatt cgtcaggat attattcac tgtggtttg 
      atgccgaag gacattcgt ctcatcaga aaaaaagac 
      acaggtact tcacgtggt ttcgccttt gtcgagttt 
      tcaacactt ggtgaggcc attcggtgg atggacatg 
      aaacagggt gttttaatg ctgcaagaa caatatcgt 
      gctataatg cagtatagc attcccaaa gacgtctgc 
      gcgaattca gaaaaacca ccaagtcat aaggcttca 
      gccgactgg ttttgcata aaatgtggc gcccagaac 
      ttcaaaaga agggacaat tgtttcaaa tgccacgct 
      tcacgaatg gaaagcgaa gaaggtggt agcggaagc 
      gacgaaata tgcacttac accaccaaa accatcatg 
      atacgcaat ctagatgcc ctgacaacg gaggattct 
      gttatgtca gttcttaat actgtcatt cctgattta 
      gtcaagtca atatcagca gtttgcatt ggtcgtgac 
      cctttgact tcaacctcc agaggcatt tgctacctt 
      ggaacagaa agcacgata gatgctttg gccatttac 
      ggagctcta agcaattta tccagcccc ctcactatt 
      gacggaaaa acagtgatt ttgtcgtat tgtaagtac 
      aacatgggt gacaacaag aaagcctat tctcaggct 
      gataacgtt gcttttccc aacgcatcc gtcccgagc 
      acttacgca atgaccgat gtggaaagt ctagcagaa 
      tatgctgcg agacgttac gctaaaact ccgagcgaa 
      tacatgcat tacattaag tattaccgc aactacttc 
      acccaacaa atcagtgcg ggcaactcg attacactc 
      catcaagaa aaccaaatg gatgcagca aacgctgca 
      gctgctgtc gcccaatcc gccatccaa caaatgcat 
      gctaataaa aatttctac gatactacc cacatgtca 
      attccaaac ggaaccgat ggcaagaga tatcctgtt 
      cctgacgtt tcaaaatat acttacgac aaaaccact 
      gggtatcaa tatgacgca agtacgggt ctatattac 
      gatccgaat tcaaactat tactggaat agcgtcatt 
      caaaaatac ctctactgg gaccaagaa aaaatgacc 
      tacgtgttg gcccccatg acttacgaa agttacacg 
      gggcagcaa accatggcc gcgtcgacc ggctcacaa 
      gaaccggaa gcgaaaaaa ccgaaagcg gaaaaacag 
      gacaaagtc aaagtcgcc aagaagatc gccaaagac 
      atggaaagg tgggcgaag acgctaaac cagaagaag 
      gagatgtcc ttcgcgcgt gctaactcc gacatgggc 
      gccagcagt agcggtagt gcgtcagcc gatgtcggt 
      ttttccgtc ctggaaaag aaaaacacg agccaattg 
      gttccaggt ttttataaa aatgactct gacgaatcc 
      attcctgca gccaccgct cctctcgtt gcagcctac 
      ggtggcgaa agcgattcc tccggagaa gaagaaatc 
      gacgagatt gagctccta gattacgcg aaacttatc 
      tgtaacctc tgcaaacgt caattaggg tccgctgaa 
      gctttaact aaacacgcc aaactatcc gccttgcac 
      aagcagaac ctggaagcg cgaaagaag acgaagaag 
      ctcgaaggc actaaagaa aaggtcgtt tacagagac 
      cgggctaag gagcgaaga atgaaatac ggtgatccg 
      gacgagcca caacccagc aaactcaaa gaaaagtat 
      ttgaagtcg agggaattg gctgaagcg ccggtcgcc 
      gcagcgtcg gtttcggaa ccgatcggg gccgaaaat 
      gtcggcaat agactacta caaaaaatg ggctggact 
      gagggccaa ggtttaggg aaacaaaat caaggaaga 
      accacaatt attcaggcg gaacagcac actagcacc 
      gtagggcta ggaaacaaa gttgcagga tatacagct 
      ctggcagga gaatcgtac aaagactgt gttaaaaaa 
      atgatgtat gcacgttac caggagttg acagaaaag 
      gaaaactaa
    mRNA sequence
    • >TC004717 RA
      ggctggcgc tttgaatgg gcgtgggaa tttttccca 
      ggctttttt tgccaactg atgtcgtaa attgcaatt 
      tttgcgatt gatttttgt cgattattt tataaaatt 
      aagggtcct ccagggggg acatgcgcc ccacgttgc 
      gctctctag tgcgcgttt ggagaagtc cacttggtg 
      ccgattgtt gttggagag agaagttcc gaggataaa 
      ttgttggcg tccttgtct aggtagtca aagtggtcg 
      tctacagag gatgataac aacgtgacg atgccagtt 
      tttgagtgt tgacgcgtt ggatggatt ttaacaatt 
      ttcagaact tatggttga aattatgaa ctgagagtt 
      tccgacttg gagcactcc aaacgcgcc gaatgtgta 
      cagaattgc ggtaaataa aaatggaaa gatctagag 
      attattcac ctagaggac gaagtatgt ccatatcac 
      cagagtaca agggttccc ccgattata gaagcaggt 
      cgccgtcgc ggtccccgc actacaaaa gacacaacg 
      ggcacagat cacccacgt ttcacagct caaaaaaat 
      ttcatcatg aatattccg acgaaagag gaaaaaatg 
      gtagtgatt atgagagtg accgcgaat accgaagag 
      agtcttctt gggatggtg accggagga gtcgttcac 
      gatctccgg actattcaa gagatcggg atagaagca 
      aacattata gaagtaggg acagggata gggaccacc 
      ataggtcta gagggaaaa ggtcgcgaa gtggttcac 
      ggtgggaac gaagatcag aagatagaa aaacacgtg 
      atcgagaca ggtcatatc gcgatgatg atagggaca 
      gtgtggata gtgaacgta gccctaacg gagctgtta 
      ttggagcta gcgggtctg aaataatag gctacaaaa 
      gtcagcccc ccaacaaca ccatcatga ttcgcggac 
      tggcgcagc acatctccg aaaacgata ttcgtcagg 
      atattattc actgtggtt tgatgccga aggacattc 
      gtctcatca gaaaaaaag acacaggta cttcacgtg 
      gtttcgcct ttgtcgagt tttcaacac ttggtgagg 
      ccattcggt ggatggaca tgaaacagg gtgttttaa 
      tgctgcaag aacaatatc gtgctataa tgcagtata 
      gcattccca aagacgtct gcgcgaatt cagaaaaac 
      caccaagtc ataaggctt cagccgact ggttttgca 
      taaaatgtg gcgcccaga acttcaaaa gaagggaca 
      attgtttca aatgccacg cttcacgaa tggaaagcg 
      aagaaggtg gtagcggaa gcgacgaaa tatgcactt 
      acaccacca aaaccatca tgatacgca atctagatg 
      ccctgacaa cggaggatt ctgttatgt cagttctta 
      atactgtca ttcctgatt tagtcaagt caatatcag 
      cagtttgca ttggtcgtg accctttga cttcaacct 
      ccagaggca tttgctacc ttggaacag aaagcacga 
      tagatgctt tggccattt acggagctc taagcaatt 
      tatccagcc ccctcacta ttgacggaa aaacagtga 
      ttttgtcgt attgtaagt acaacatgg gtgacaaca 
      agaaagcct attctcagg ctgataacg ttgcttttc 
      ccaacgcat ccgtcccga gcacttacg caatgaccg 
      atgtggaaa gtctagcag aatatgctg cgagacgtt 
      acgctaaaa ctccgagcg aatacatgc attacatta 
      agtattacc gcaactact tcacccaac aaatcagtg 
      cgggcaact cgattacac tccatcaag aaaaccaaa 
      tggatgcag caaacgctg cagctgctg tcgcccaat 
      ccgccatcc aacaaatgc atgctaata aaaatttct 
      acgatacta cccacatgt caattccaa acggaaccg 
      atggcaaga gatatcctg ttcctgacg tttcaaaat 
      atacttacg acaaaacca ctgggtatc aatatgacg 
      caagtacgg gtctatatt acgatccga attcaaact 
      attactgga atagcgtca ttcaaaaat acctctact 
      gggaccaag aaaaaatga cctacgtgt tggccccca 
      tgacttacg aaagttaca cggggcagc aaaccatgg 
      ccgcgtcga ccggctcac aagaaccgg aagcgaaaa 
      aaccgaaag cggaaaaac aggacaaag tcaaagtcg 
      ccaagaaga tcgccaaag acatggaaa ggtgggcga 
      agacgctaa accagaaga aggagatgt ccttcgcgc 
      gtgctaact ccgacatgg gcgccagca gtagcggta 
      gtgcgtcag ccgatgtcg gtttttccg tcctggaaa 
      agaaaaaca cgagccaat tggttccag gtttttata 
      aaaatgact ctgacgaat ccattcctg cagccaccg 
      ctcctctcg ttgcagcct acggtggcg aaagcgatt 
      cctccggag aagaagaaa tcgacgaga ttgagctcc 
      tagattacg cgaaactta tctgtaacc tctgcaaac 
      gtcaattag ggtccgctg aagctttaa ctaaacacg 
      ccaaactat ccgccttgc acaagcaga acctggaag 
      cgcgaaaga agacgaaga agctcgaag gcactaaag 
      aaaaggtcg tttacagag accgggcta aggagcgaa 
      gaatgaaat acggtgatc cggacgagc cacaaccca 
      gcaaactca aagaaaagt atttgaagt cgagggaat 
      tggctgaag cgccggtcg ccgcagcgt cggtttcgg 
      aaccgatcg gggccgaaa atgtcggca atagactac 
      tacaaaaaa tgggctgga ctgagggcc aaggtttag 
      ggaaacaaa atcaaggaa gaaccacaa ttattcagg 
      cggaacagc acactagca ccgtagggc taggaaaca 
      aagttgcag gatatacag ctctggcag gagaatcgt 
      acaaagact gtgttaaaa aaatgatgt atgcacgtt 
      accaggagt tgacagaaa aggaaaact aatcaggtt 
      aatcagttg tttcactga cttttgtca agtgccaag 
      cagtgttaa ttaattttt catccaaac atcatgtta 
      ataaattag aagtaagtt a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY