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Gene information
General information
TC013586 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3402825..3424672
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013586
    iBeetle screen iB_02945

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013586 RA
      MAATQAEKH QNDVNCEKV QPDQHLNVR NSLLQNGTD 
      KSRVRGFCG VAVSKAKNT GGKNLDTEM SQYRSDGSG 
      GGPAAPTAP DPGPDGEPG PGSAPPDAA AFPVEGPPA 
      DGHGYGFSY GRDVVHNTA EGVHAFGPR QALGGSKQA 
      PAPGAPYPQ RYVSGQTLS QPTGPTPTL NQLLQSSNP 
      MPPHRYQNS YGHPDQPYN QGWPPQKPM PTYGTPPPP 
      PQPGVPPAP YRNQPTLSP AYSGGSAPS PGYGEGRSG 
      WAGPPVSGQ HGPGSPGPP NTTAPASQP SPQPPISHS 
      PGPGLPPSP QHQQQQQPQ HQGFPNRPA QPTTPNAHA 
      PDAADLSGG NSNDSSGGP APGTPNSQG MRPTPSPTG 
      STGSRSMSP AVGQQNIPM PPRPSSGQS DGSGSTRMS 
      HSPMATQGS YSSPHPAHV HGYKGHPGM VSGPGPGPG 
      PAGQQMPLY QPNQQYPQG GYPPRPQAN IQYPGQGYG 
      PPGYPNRSS NHMPNSQFQ PYQQPWQGP MTLAKGNGP 
      PQAPGSPSP GPPGPPPRP PHYLKQHLQ HKMGFGNVP 
      GSAPPSPSP PQNYHMGPP PAGHHHSGM GPPPTMGPP 
      PNMPPASSP LMPNSHSHD GPMPPPSST PNSHTQMGS 
      EMMDNGITT TAQGGMSTH VTTAAGGSV TSVVTTGPD 
      GTPIDEGSQ QSTLSNASA ASGEDPQCT TPKSNRKNE 
      MGHYSHPTT PQSTVPSPG AASMNSMHD DYEISSPSW 
      PRTPASPKT DSLSKLYEM DDSPDRRAW LDKLLTFME 
      ERRSPITTC PTISKNPLD LFRLYVYVK DRGGFLEVT 
      KNKTWKDIA GMLGIGASS SAAYTLRKH YTKNLLAYE 
      CQFDRGGID PQPIINQVE ATSKKKSAK AASVPSPGS 
      SNSQDSFPP PGSGGTSMD GYGYGYPPA GNPEYNPQQ 
      RPSSQSNAP SPHGGSGGA NHLNNAAPG GPSPAGGAA 
      DNVSVSNPF DDVSPSPPP PRGGPFPGG PHPPYPSGT 
      PPRPQGFSG QAGYNQYPS PGPGAPPDQ YSTYPASSG 
      YPPAVRPVY PPYSGDSGA PPPPNPNNT PPGGPPAAG 
      QDPYSRYNM VGAAAPPTS TYTPRPNYT GAPGNTGPP 
      TSQPPAGPY TTQQDYYRP EQNYPNQQG PSATGNYPG 
      GPPTNKNMP PPGPQPTRR HPDFVKDQQ TYPFGQQRP 
      HIYAWSNNN QYRPPQQYG ASGGPQVGT QQWNQGGRP 
      AAGGQWDRY PSGNQGYQP PQSGQGQWS SIPAPGVGQ 
      NPPLRPPLG GPRPGKPFT MMPPQGGKG NQATTLSNS 
      SYSQSQLPK REITFPPDS VEATLPVLY RRKRLCRAD 
      IGQVDPWRI IMCLRSGLL SESTYALDI LNVLLYDDS 
      SVGYFGLNQ WPGLLDILI EHFQKALSD IFDGPYPRE 
      EKPDESDVD LGGVVTPID PNKKSVLLQ NTNNYTFVT 
      RKGYPVKMI DRSEEIFIQ DHQKDWDVK GDATGASIL 
      AEMTTDPWQ SRTDHILPT FQAEFGRIP FHRRLEDKK 
      KDRNSNNKN NSRESIEER QRTPSPPDE APPPPKTSD 
      KKRRTKTLS DVISRIKKD SSSTEANGV LTLETTTVT 
      VTEKVKAEV VVNCEEQSV DLNAGSVNG ECGKDNTSS 
      SNSSGPQVR DPVGRLKRR RSLDCEDEA YTRDEASLV 
      LITESQDNI GKRCVCISN ILRSLTFIP GNESEFSRS 
      STFLAIVGK LLLLHHEHP PRTQKTRNY DREEDADFA 
      DSCSSLQGE SEWWWDFLI QIRENILVS IANISGQID 
      FNVYPEEIV RPILDGLLH WAICPSATG QDPFASVGP 
      SSLLSPQRL ALESLCKLC VTNNNVDLV IATPPFSRL 
      ERLCAVLTK HLCRSEDQV LREFSINLL HYLAAADSS 
      MARTVASQT PCVSLLVAF IEQAEQSAL GVANQHGIG 
      ALRDNPDSM GTSLDMLRR AAATLVFLA RHPDNKPLL 
      VQQESRLLA LVMSQILDQ QVALLLSKV LFQISRS
    CDS sequence
    • >TC013586 RA
      atggctgca acgcaggcc gaaaagcac caaaatgac 
      gtgaattgt gagaaagtg cagccggat cagcatctg 
      aacgtgcgg aattccttg ttgcaaaat gggaccgat 
      aagagtaga gttcgcgga ttttgcggt gtagcggta 
      tccaaggcg aaaaatacc ggcggcaaa aacctcgac 
      actgaaatg agtcaatat cgctcggac ggcagcggc 
      ggtgggcct gcggccccc acggccccc gacccgggc 
      cccgacggc gagccgggg ccgggcagt gcgcccccc 
      gacgcggcc gccttcccc gtcgagggg ccccccgcc 
      gacgggcac ggttacggc ttctcctac ggccgcgac 
      gtcgtgcac aacaccgcc gagggcgtc cacgcgttc 
      ggtccgcgg caggcgctg gggggctcc aagcaggcg 
      cccgcgccc ggggcgccc tatccccag agatacgtt 
      tcggggcag acgctctcg cagcccacc ggcccgacc 
      cctacgctc aaccagctg ctgcagagc tccaacccg 
      atgcctccg catcgatac cagaacagc tacggccac 
      cccgaccag ccgtataac caggggtgg ccgccccag 
      aagccgatg cccacctat gggactccc ccgccgccg 
      ccacaaccg ggagtcccg ccggcacca taccgcaac 
      caacctacg ctatcgccg gcgtacagc ggcggttcg 
      gcgcccagt cctggttac ggggagggc cgttcgggg 
      tgggccgga cccccggtg tcgggccag cacggcccc 
      ggaagcccc ggccctcca aacacgacg gcaccggca 
      agtcagccc tcgccgcag cctcccatc agtcactcc 
      ccggggcct ggtcttccg cccagcccc caacaccag 
      cagcaacag cagccccag caccagggt ttcccgaac 
      cggccggcc cagccaact acccccaac gcacacgcg 
      ccagatgca gcggacttg agcggcggt aacagcaat 
      gacagttcc ggaggacca gctccggga acaccaaac 
      tcccaaggg atgaggccc acaccttct cccacggga 
      tcgacagga tcgcgctct atgtctccg gctgtcggt 
      cagcaaaac attccgatg cctcctcgg ccgtccagt 
      ggtcaatca gatggcagt ggatcaact cgaatgagc 
      cattcgccg atggcgacg caaggcagc tattcgtcg 
      cctcatccg gcccacgtt cacggttat aagggccat 
      ccgggcatg gtgtccggc ccagggcct ggccccggc 
      cccgccggc cagcagatg ccgctttat cagcccaac 
      cagcagtac ccgcaaggc ggttatccg ccgaggccc 
      caagccaac atccagtat cccggccag ggctacggc 
      ccccccggc tatccgaat cggtcgtcc aaccacatg 
      ccgaattcg caatttcag ccgtatcaa caaccgtgg 
      caaggaccg atgacgtta gcgaaaggt aacggaccg 
      ccgcaagcc ccaggatca ccttcgccc ggtcccccg 
      ggaccgccg ccgcgacct ccgcactac ctcaaacaa 
      cacctacaa cacaaaatg ggcttcggt aacgttccc 
      ggatcggcg ccaccgagt ccaagtccg ccgcagaat 
      taccacatg ggtccgccg ccggcggga caccaccat 
      tcggggatg ggaccgccg cccactatg ggaccgccg 
      ccgaatatg ccacccgcg agcagtccc ctaatgcct 
      aacagccat tcacatgac ggtcccatg ccgccaccg 
      agttcgaca ccgaattca catacgcaa atgggttct 
      gaaatgatg gataatggg attacgaca acggcacaa 
      ggtgggatg agtactcat gtcacaacg gcggcggga 
      ggatcggtt acttccgtt gttacaaca ggacctgat 
      gggacaccg atcgatgaa ggatcgcaa caatcgaca 
      ctgtcaaat gcatcagcc gcttccggc gaagatccc 
      cagtgtacc acaccgaaa tcgaatcgg aaaaatgaa 
      atgggtcac tatagccat ccaacaacg ccgcaaagt 
      acagtgcca tctcctggt gcagcaagt atgaattca 
      atgcatgac gactacgaa ataagcagt ccaagttgg 
      ccaaggacc cccgcaagt ccaaaaact gatagtctc 
      agcaagtta tacgaaatg gatgacagt ccagataga 
      cgtgcttgg ttggataaa ctactcaca tttatggaa 
      gagagaaga tcgccaatc actacttgc cctactatt 
      tccaagaac ccgcttgat ttgttccgt ttgtatgtc 
      tatgttaag gacaggggt ggttttctg gaggttacg 
      aaaaacaaa acatggaaa gatatcgcc ggaatgttg 
      ggtatcggt gcatcttca agtgcagca tacacattg 
      aggaaacac tatactaaa aatttatta gcttacgaa 
      tgtcagttc gatcgagga ggaattgat cctcaacct 
      ataataaat caagttgaa gcaacttcg aaaaagaaa 
      tcagctaaa gccgcctcg gtgccttca ccaggctct 
      tccaattct caggattct tttccgcca cctggttcc 
      ggcggtaca tcaatggac ggatacggt tacggatat 
      ccaccagct ggaaatcca gaatataat cctcaacag 
      aggccatct tcacaatct aacgcgcct tctccgcac 
      ggaggttca ggaggggct aatcattta aacaatgcc 
      gcccctggg ggtcctagc cctgcaggc ggggctgcc 
      gacaacgta agcgtttca aaccctttt gatgatgtg 
      tctcccagt ccaccacca ccacggggt ggccccttt 
      ccaggtgga ccgcaccct ccttatcct tcaggcact 
      ccacctaga ccgcaggga tttagcgga caggctgga 
      tacaatcag tatccaagt cctggtcct ggtgctcct 
      ccagatcag tattctacg tacccagct agttcaggt 
      tatccacca gccgttaga ccagtgtat cctccatat 
      tcaggtgat tcaggagca ccgccgccg ccgaatccg 
      aacaataca ccacctggt ggtccgccg gctgccgga 
      caagaccca tattcacgt tataatatg gttggtgca 
      gctgcacca ccaacatcc acatacaca ccgaggcca 
      aactacaca ggcgcaccg ggaaataca gggccgcca 
      acctcgcaa ccgccagcc ggaccgtac acgacacag 
      caagattat tacagaccg gaacagaat tatcccaat 
      cagcagggt ccttcggca acgggtaat tatcccgga 
      ggaccgccg actaacaag aatatgcca ccgcctgga 
      ccgcaacca acaaggcga catccagac ttcgtcaaa 
      gatcagcag acctatccc ttcggtcag caaaggcca 
      catatttat gcatggtcg aataacaac cagtacagg 
      ccgccacag cagtatggg gcatctgga ggtccccaa 
      gtgggtaca cagcagtgg aatcaggga ggaagaccg 
      gcggctggt ggtcagtgg gatcgttat ccgtcgggt 
      aatcaaggt tatcagcct ccacaatcc gggcaaggg 
      caatggtct tcaatccca gcacctggt gttggtcag 
      aatccaccg ctgaggcca cctcttgga ggtccacgt 
      cctggtaaa ccgtttacg atgatgcca ccgcaagga 
      ggtaaagga aaccaagcg acaacgttg tcgaatagt 
      tcgtattcg caaagtcag ttaccgaag agagaaata 
      acgtttcct cctgatagt gttgaagca acattgccg 
      gtattatac agacgtaaa agattatgc agagctgac 
      attggtcaa gttgatcct tggcgtata ataatgtgt 
      ttgcgttct ggactttta tcagaaagc acgtatgct 
      ttggacatt ttaaatgtt ttgttatat gacgattca 
      tccgttggc tacttcgga ttgaatcaa tggcctggt 
      cttttggac atattgatt gaacacttc caaaaggcg 
      ttaagtgac atttttgat ggaccttat ccacgagaa 
      gagaaacca gatgaatct gacgttgat ttgggaggt 
      gttgtaaca ccgattgat cccaataaa aaatctgtt 
      ttattgcaa aatacaaac aactataca tttgtgaca 
      cggaaaggt tatcctgtt aaaatgatc gatcgtagc 
      gaggaaatc ttcattcag gatcatcag aaagactgg 
      gatgtgaaa ggtgatgct accggtgct agtatttta 
      gctgaaatg acaacggat ccttggcaa tcaaggacg 
      gatcacata cttccaacg tttcaagcg gaatttggt 
      cgaataccg ttccatcga cgtctagaa gataaaaag 
      aaagatcgc aacagcaac aacaagaac aacagcagg 
      gagtcaata gaggagcgt caacgaaca ccgtcgccg 
      cctgatgag gcgccaccg cctcctaaa actagtgac 
      aaaaaacgt aggacaaag acattaagt gatgtgata 
      tctcgaatt aaaaaagat tcttcttca actgaagcg 
      aacggtgtg ctcacatta gagacgaca actgtaact 
      gtaactgaa aaagttaag gctgaggta gtagtgaac 
      tgtgaagaa caaagtgta gatctgaac gcaggtagt 
      gtgaacggg gagtgcggg aaagataat actagtagt 
      agtaatagt agtggacca caagtgcga gatcctgtc 
      ggaagattg aaacgtcgt cggtctttg gattgcgaa 
      gacgaagca tacactaga gacgaagca agtctagtg 
      cttataact gaaagccag gacaatatc ggaaaaaga 
      tgcgtatgc atatctaat atacttagg agccttacg 
      tttattccc ggcaacgag agcgaattc tcacgtagt 
      tcgactttc ttggcgatt gtgggaaag ctgctcttg 
      cttcatcat gagcatcca cctcgcaca cagaaaacg 
      agaaattac gaccgtgag gaggatgcc gactttgcc 
      gattcttgt agcagtttg caaggtgaa agtgaatgg 
      tggtgggat ttcctaatt caaattcga gagaacata 
      ctggtgtca atagccaac atttcgggt cagatcgat 
      ttcaacgtg tatcctgag gagatagtg aggcctata 
      ttggacggt ttactgcac tgggcgatt tgtccttcg 
      gcaaccggc caagacccg ttcgcttca gtgggcccc 
      tcatcgctg ctgtctccg caacggttg gcactggag 
      tcgctgtgc aagctttgt gttacaaac aataacgtc 
      gatttggtg atagcaacg ccgccgttt tcgcgattg 
      gagcggctt tgcgcggtg ctgacgaaa cacctttgt 
      cggtccgaa gatcaagtg ttgcgcgaa ttttcgata 
      aatctgttg cattatctg gcggcggct gacagcagc 
      atggcacgg acggtggcg tcgcaaacg ccgtgcgtt 
      tcgctgctg gtcgcgttc atcgagcaa gccgaacaa 
      agcgctttg ggcgtcgcg aaccaacat ggtataggt 
      gcactacga gataatccc gattcgatg ggaacgagt 
      ctggatatg ttgaggaga gcggcggcg acgttagtg 
      tttctggcg cggcatccg gataacaaa ccgttgttg 
      gtccaacaa gaatcgagg ctgttggcg cttgttatg 
      agtcaaatt ttagatcag caggtggca ctcctgctt 
      tccaaagta ctatttcaa atatctcgc agctaa
    mRNA sequence
    • >TC013586 RA
      cgaaaagta gatctgtgc tttagaaat ttttgtgtg 
      gctttacgg cgtttacct ggcgattat ttttggaga 
      gatggtata cttgtaatg tcggtgagt tacgtttgc 
      gttatttgt aatgggtgt gtgttagaa atgcgttaa 
      ttacatgat aatatgtcg gcgtaggaa tcggagctt 
      tacccataa cctcctttc gtgttattg gagtagata 
      cggaattaa gttgtgtgt gtcagatgt gtccatcat 
      tttggatta ttcaaaaag tttggtggc gttatttat 
      ttacagaat cggaaaagt gttaaagtg agtaatcta 
      gtatgtgtt tgctaggac ccgggtgcg attcgggct 
      cccaaaatg aaggaaagc atccgttgc tgactataa 
      cggcggggt gttggcaac gatcggccg tgctagatc 
      gcagccgtt tttcttaac atggctgca acgcaggcc 
      gaaaagcac caaaatgac gtgaattgt gagaaagtg 
      cagccggat cagcatctg aacgtgcgg aattccttg 
      ttgcaaaat gggaccgat aagagtaga gttcgcgga 
      ttttgcggt gtagcggta tccaaggcg aaaaatacc 
      ggcggcaaa aacctcgac actgaaatg agtcaatat 
      cgctcggac ggcagcggc ggtgggcct gcggccccc 
      acggccccc gacccgggc cccgacggc gagccgggg 
      ccgggcagt gcgcccccc gacgcggcc gccttcccc 
      gtcgagggg ccccccgcc gacgggcac ggttacggc 
      ttctcctac ggccgcgac gtcgtgcac aacaccgcc 
      gagggcgtc cacgcgttc ggtccgcgg caggcgctg 
      gggggctcc aagcaggcg cccgcgccc ggggcgccc 
      tatccccag agatacgtt tcggggcag acgctctcg 
      cagcccacc ggcccgacc cctacgctc aaccagctg 
      ctgcagagc tccaacccg atgcctccg catcgatac 
      cagaacagc tacggccac cccgaccag ccgtataac 
      caggggtgg ccgccccag aagccgatg cccacctat 
      gggactccc ccgccgccg ccacaaccg ggagtcccg 
      ccggcacca taccgcaac caacctacg ctatcgccg 
      gcgtacagc ggcggttcg gcgcccagt cctggttac 
      ggggagggc cgttcgggg tgggccgga cccccggtg 
      tcgggccag cacggcccc ggaagcccc ggccctcca 
      aacacgacg gcaccggca agtcagccc tcgccgcag 
      cctcccatc agtcactcc ccggggcct ggtcttccg 
      cccagcccc caacaccag cagcaacag cagccccag 
      caccagggt ttcccgaac cggccggcc cagccaact 
      acccccaac gcacacgcg ccagatgca gcggacttg 
      agcggcggt aacagcaat gacagttcc ggaggacca 
      gctccggga acaccaaac tcccaaggg atgaggccc 
      acaccttct cccacggga tcgacagga tcgcgctct 
      atgtctccg gctgtcggt cagcaaaac attccgatg 
      cctcctcgg ccgtccagt ggtcaatca gatggcagt 
      ggatcaact cgaatgagc cattcgccg atggcgacg 
      caaggcagc tattcgtcg cctcatccg gcccacgtt 
      cacggttat aagggccat ccgggcatg gtgtccggc 
      ccagggcct ggccccggc cccgccggc cagcagatg 
      ccgctttat cagcccaac cagcagtac ccgcaaggc 
      ggttatccg ccgaggccc caagccaac atccagtat 
      cccggccag ggctacggc ccccccggc tatccgaat 
      cggtcgtcc aaccacatg ccgaattcg caatttcag 
      ccgtatcaa caaccgtgg caaggaccg atgacgtta 
      gcgaaaggt aacggaccg ccgcaagcc ccaggatca 
      ccttcgccc ggtcccccg ggaccgccg ccgcgacct 
      ccgcactac ctcaaacaa cacctacaa cacaaaatg 
      ggcttcggt aacgttccc ggatcggcg ccaccgagt 
      ccaagtccg ccgcagaat taccacatg ggtccgccg 
      ccggcggga caccaccat tcggggatg ggaccgccg 
      cccactatg ggaccgccg ccgaatatg ccacccgcg 
      agcagtccc ctaatgcct aacagccat tcacatgac 
      ggtcccatg ccgccaccg agttcgaca ccgaattca 
      catacgcaa atgggttct gaaatgatg gataatggg 
      attacgaca acggcacaa ggtgggatg agtactcat 
      gtcacaacg gcggcggga ggatcggtt acttccgtt 
      gttacaaca ggacctgat gggacaccg atcgatgaa 
      ggatcgcaa caatcgaca ctgtcaaat gcatcagcc 
      gcttccggc gaagatccc cagtgtacc acaccgaaa 
      tcgaatcgg aaaaatgaa atgggtcac tatagccat 
      ccaacaacg ccgcaaagt acagtgcca tctcctggt 
      gcagcaagt atgaattca atgcatgac gactacgaa 
      ataagcagt ccaagttgg ccaaggacc cccgcaagt 
      ccaaaaact gatagtctc agcaagtta tacgaaatg 
      gatgacagt ccagataga cgtgcttgg ttggataaa 
      ctactcaca tttatggaa gagagaaga tcgccaatc 
      actacttgc cctactatt tccaagaac ccgcttgat 
      ttgttccgt ttgtatgtc tatgttaag gacaggggt 
      ggttttctg gaggttacg aaaaacaaa acatggaaa 
      gatatcgcc ggaatgttg ggtatcggt gcatcttca 
      agtgcagca tacacattg aggaaacac tatactaaa 
      aatttatta gcttacgaa tgtcagttc gatcgagga 
      ggaattgat cctcaacct ataataaat caagttgaa 
      gcaacttcg aaaaagaaa tcagctaaa gccgcctcg 
      gtgccttca ccaggctct tccaattct caggattct 
      tttccgcca cctggttcc ggcggtaca tcaatggac 
      ggatacggt tacggatat ccaccagct ggaaatcca 
      gaatataat cctcaacag aggccatct tcacaatct 
      aacgcgcct tctccgcac ggaggttca ggaggggct 
      aatcattta aacaatgcc gcccctggg ggtcctagc 
      cctgcaggc ggggctgcc gacaacgta agcgtttca 
      aaccctttt gatgatgtg tctcccagt ccaccacca 
      ccacggggt ggccccttt ccaggtgga ccgcaccct 
      ccttatcct tcaggcact ccacctaga ccgcaggga 
      tttagcgga caggctgga tacaatcag tatccaagt 
      cctggtcct ggtgctcct ccagatcag tattctacg 
      tacccagct agttcaggt tatccacca gccgttaga 
      ccagtgtat cctccatat tcaggtgat tcaggagca 
      ccgccgccg ccgaatccg aacaataca ccacctggt 
      ggtccgccg gctgccgga caagaccca tattcacgt 
      tataatatg gttggtgca gctgcacca ccaacatcc 
      acatacaca ccgaggcca aactacaca ggcgcaccg 
      ggaaataca gggccgcca acctcgcaa ccgccagcc 
      ggaccgtac acgacacag caagattat tacagaccg 
      gaacagaat tatcccaat cagcagggt ccttcggca 
      acgggtaat tatcccgga ggaccgccg actaacaag 
      aatatgcca ccgcctgga ccgcaacca acaaggcga 
      catccagac ttcgtcaaa gatcagcag acctatccc 
      ttcggtcag caaaggcca catatttat gcatggtcg 
      aataacaac cagtacagg ccgccacag cagtatggg 
      gcatctgga ggtccccaa gtgggtaca cagcagtgg 
      aatcaggga ggaagaccg gcggctggt ggtcagtgg 
      gatcgttat ccgtcgggt aatcaaggt tatcagcct 
      ccacaatcc gggcaaggg caatggtct tcaatccca 
      gcacctggt gttggtcag aatccaccg ctgaggcca 
      cctcttgga ggtccacgt cctggtaaa ccgtttacg 
      atgatgcca ccgcaagga ggtaaagga aaccaagcg 
      acaacgttg tcgaatagt tcgtattcg caaagtcag 
      ttaccgaag agagaaata acgtttcct cctgatagt 
      gttgaagca acattgccg gtattatac agacgtaaa 
      agattatgc agagctgac attggtcaa gttgatcct 
      tggcgtata ataatgtgt ttgcgttct ggactttta 
      tcagaaagc acgtatgct ttggacatt ttaaatgtt 
      ttgttatat gacgattca tccgttggc tacttcgga 
      ttgaatcaa tggcctggt cttttggac atattgatt 
      gaacacttc caaaaggcg ttaagtgac atttttgat 
      ggaccttat ccacgagaa gagaaacca gatgaatct 
      gacgttgat ttgggaggt gttgtaaca ccgattgat 
      cccaataaa aaatctgtt ttattgcaa aatacaaac 
      aactataca tttgtgaca cggaaaggt tatcctgtt 
      aaaatgatc gatcgtagc gaggaaatc ttcattcag 
      gatcatcag aaagactgg gatgtgaaa ggtgatgct 
      accggtgct agtatttta gctgaaatg acaacggat 
      ccttggcaa tcaaggacg gatcacata cttccaacg 
      tttcaagcg gaatttggt cgaataccg ttccatcga 
      cgtctagaa gataaaaag aaagatcgc aacagcaac 
      aacaagaac aacagcagg gagtcaata gaggagcgt 
      caacgaaca ccgtcgccg cctgatgag gcgccaccg 
      cctcctaaa actagtgac aaaaaacgt aggacaaag 
      acattaagt gatgtgata tctcgaatt aaaaaagat 
      tcttcttca actgaagcg aacggtgtg ctcacatta 
      gagacgaca actgtaact gtaactgaa aaagttaag 
      gctgaggta gtagtgaac tgtgaagaa caaagtgta 
      gatctgaac gcaggtagt gtgaacggg gagtgcggg 
      aaagataat actagtagt agtaatagt agtggacca 
      caagtgcga gatcctgtc ggaagattg aaacgtcgt 
      cggtctttg gattgcgaa gacgaagca tacactaga 
      gacgaagca agtctagtg cttataact gaaagccag 
      gacaatatc ggaaaaaga tgcgtatgc atatctaat 
      atacttagg agccttacg tttattccc ggcaacgag 
      agcgaattc tcacgtagt tcgactttc ttggcgatt 
      gtgggaaag ctgctcttg cttcatcat gagcatcca 
      cctcgcaca cagaaaacg agaaattac gaccgtgag 
      gaggatgcc gactttgcc gattcttgt agcagtttg 
      caaggtgaa agtgaatgg tggtgggat ttcctaatt 
      caaattcga gagaacata ctggtgtca atagccaac 
      atttcgggt cagatcgat ttcaacgtg tatcctgag 
      gagatagtg aggcctata ttggacggt ttactgcac 
      tgggcgatt tgtccttcg gcaaccggc caagacccg 
      ttcgcttca gtgggcccc tcatcgctg ctgtctccg 
      caacggttg gcactggag tcgctgtgc aagctttgt 
      gttacaaac aataacgtc gatttggtg atagcaacg 
      ccgccgttt tcgcgattg gagcggctt tgcgcggtg 
      ctgacgaaa cacctttgt cggtccgaa gatcaagtg 
      ttgcgcgaa ttttcgata aatctgttg cattatctg 
      gcggcggct gacagcagc atggcacgg acggtggcg 
      tcgcaaacg ccgtgcgtt tcgctgctg gtcgcgttc 
      atcgagcaa gccgaacaa agcgctttg ggcgtcgcg 
      aaccaacat ggtataggt gcactacga gataatccc 
      gattcgatg ggaacgagt ctggatatg ttgaggaga 
      gcggcggcg acgttagtg tttctggcg cggcatccg 
      gataacaaa ccgttgttg gtccaacaa gaatcgagg 
      ctgttggcg cttgttatg agtcaaatt ttagatcag 
      caggtggca ctcctgctt tccaaagta ctatttcaa 
      atatctcgc agctaatcc ctctgtaga tactcttgc 
      ggccttgga ctggtgtac agacctcta tgtatagcg 
      tgaatagtt aaactttac caatgaaca gtgtgtatg 
      tgtgagtgt aatgggaca aatggcgag agtcgccgt 
      gagatccgg atctccgat agctaatga attcaccta 
      gtgtaacca acccataat tgtaccatt ttgtaaatt 
      aagcaaaca tacgtcttt ctggaaatg tcaaacatc 
      catgttact tatgtattt attaatttg aataacaga 
      gtatggtag tgctgtagc tctcgagtt gctgcctgg 
      tatgatgat gataatgat aataacgaa acggtgatg 
      atgatggtg gtcatccct tttattttt cctgcgcac 
      tcagttctc ttatttcat gtttggtat tactgtgcg 
      aatgcttta tacatgtct gataatgaa ataaataaa 
      tgtacaaag gaagtaccg aaaatggtg tttttatac 
      aagttttaa ttttctatg tatatttag aaaatgttt 
      tatacatcc cattttaga tgttacatt gaactctgt 
      taatgaaca agtatggac tgtaaaaca atgcaactt 
      gcgttatta gaaaaagca gtcttgcta tagaactca 
      aatgtagtt taaaatatg tggtgatat ttttctggc 
      tgtaggtat atttcatct tatgtatga ttgcatatt 
      gtaaaaatt aagtaaatg ctaaataca ttaaattct 
      tttta
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