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Gene information
General information
TC004153 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 1483661..1486887
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004153
    iBeetle screen iB_02943
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004153 RA
      MCTDEDIFW YHGKLSREA TENLLKEEG RPDGSFLVR 
      ESNSSTGDF VLSVLHNNE VSHFQIRRH SEDAFFSID 
      ENTKIHGLE SLIEHYVNT KGILSEGLS LTVPVKGDA 
      PPDYSRRHG RTNLLHRAT KEGNYKVVS EVLKTGYRH 
      EAKNQNGQT AVHLASMNG KDDILRKLI DYGASVNLR 
      DTAGYTPLH YACQNNFPS TVRLLVQVA NANIQSRNT 
      ETGAVPLHE AASRGHKDV VKALLSLNA PVNPRDKDN 
      QLPSHLARK NGFIECAEI LENYQCPAP KTHRSYWYH 
      GTLDRHEAE TTIKQFSTK DGTFLVRWS DRNKERVLT 
      LINESLFYN YIIRKQDDY LFIDNGPYL ESLEHIIEY 
      YSFIPDGLP TVLQTPVPP KPKPPVPEC TTIPRQRKK 
      NLRPQPEII SIAESQSLK CFPSQFQNI HFTTNFSNN 
      NNYTTNDNE DDYIPLERL KMGRVIGEG EFGSVYEGT 
      YTKRNGEEI KVAIKTVRH EQIETSNAI LKEAHVMMR 
      LNHHCVVKL IGLSKGRPL LMVQELVAL GSMLNYIIL 
      NKESINPNS EFKIWAAQI ACGMNYLEE QRFIHRDLA 
      ARNILLASQ HQAKISDFG LSRALAADH EYYRALQGG 
      KWPLKWYAP ESYNYGQFS HKSDVWSFG VAIWEMYSF 
      GDVPYGELK GSDAIKIIE DGERLKQPD ACPDHIYEI 
      MRRCWEYDA ENRPTFKEL LEFFSSDTE YMNIRELLQ 
      SANLA
    CDS sequence
    • >TC004153 RA
      atgtgcaca gatgaggac attttttgg tatcacgga 
      aaattgtca cgggaagcc acggaaaac ctattaaaa 
      gaagaggga cgtcccgat gggagtttt ttagtgcgt 
      gagagtaac tcatcaacg ggcgatttc gtactctca 
      gtccttcac aacaatgaa gttagtcat tttcaaatc 
      cgtagacac agcgaagat gcgtttttt tcaatagat 
      gaaaatact aaaatccac ggccttgag agtctgata 
      gagcactac gtaaacaca aaggggatc ctctcagag 
      ggcctgtct ctgactgtt cccgtcaaa ggggacgcg 
      ccccccgac tattcacgc agacacggc cgcacgaat 
      ttgctgcac cgcgccaca aaagaaggg aattacaaa 
      gtcgtgtct gaggttctt aagaccggc tataggcac 
      gaggcgaag aaccagaac ggccaaacg gccgtgcac 
      ttagccagc atgaacggc aaggacgac attctcaga 
      aaactcata gactacggt gctagtgtt aatttacga 
      gatactgcc ggatacact cctcttcat tatgcttgc 
      caaaacaat tttccaagt actgtaaga ctcctggtc 
      caagtggcc aacgctaac atccagtcc cggaacacc 
      gaaactggg gccgttcct ttacacgaa gctgccagt 
      cggggccac aaagacgtg gtcaaagca ttactaagc 
      ctaaatgcc ccggttaat ccccgggat aaggataac 
      caattgcca tcgcatctt gctaggaaa aatgggttc 
      atagaatgt gccgaaatt ttagaaaac tatcaatgt 
      ccagcaccc aaaacgcat aggagttac tggtaccac 
      ggtacccta gaccgacac gaagccgaa accacgatc 
      aagcaattc agtacgaaa gacggcacg tttttagtc 
      aggtggagc gaccgcaac aaagaacgt gtcctcacc 
      cttataaac gagtcgttg ttttataat tacataatt 
      aggaaacag gatgattat ttatttatc gacaatggg 
      ccttactta gaatcctta gagcatatt attgaatat 
      tatagtttt atccctgac ggcttgccg acagtgtta 
      caaacacct gtacctcct aaacctaag cccccggtc 
      cctgaatgt accacaata cccagacaa cgaaagaaa 
      aaccttaga ccgcaacca gaaataata agtatagcc 
      gaatctcag tcgcttaag tgttttcct agtcaattt 
      caaaacatc catttcacg acaaatttt tcgaataat 
      aataactac acaacgaac gacaacgaa gatgactat 
      ataccgcta gaaaggcta aaaatgggt cgggttatt 
      ggggagggc gagttcggt tcggtctac gaaggtact 
      tatacgaaa cggaacgga gaggaaatc aaagtcgct 
      ataaaaaca gtcagacac gagcaaatc gaaacgagt 
      aatgccatt ttgaaggaa gcccacgtg atgatgagg 
      ctgaaccac cactgcgtg gttaagctc atcgggctg 
      tcgaaaggg cgcccgctc ctgatggtg caggaacta 
      gtcgcgcta gggtctatg ttgaactac attattctc 
      aataaggag agcattaat ccgaactcg gagttcaag 
      atttgggcc gctcagata gcttgcggg atgaattat 
      ttagaagaa caaagattt attcatagg gacctagcc 
      gcaaggaat atccttcta gcgtcccag caccaggca 
      aaaatcagc gactttggc ctgtctaga gcgttggcc 
      gctgatcat gaatattac agagctttg cagggcggg 
      aaatggcca ctgaagtgg tacgccccc gagtcgtac 
      aactacggc cagttttca cacaaaagt gatgtgtgg 
      agtttcggc gtcgccatc tgggaaatg tacagtttc 
      ggtgacgtg ccttacggc gaattgaag gggtctgat 
      gcgatcaaa attatcgaa gacggcgag cggttgaag 
      caaccggac gcttgtccg gaccatatt tacgaaata 
      atgcggcgg tgttgggag tacgacgcg gagaacagg 
      ccgacgttc aaggagctg ttggagttt ttctcgtcg 
      gatacggag tacatgaac atacgggag ttgctgcag 
      agtgccaac ctggcctga
    mRNA sequence
    • >TC004153 RA
      cgattaatt tgacatttt tccttttgt aatagtgaa 
      caataatat ttattgtcg aaatagtaa aatgatttt 
      ctgagtgtt gtttttaca tgtggttga ctcagcagg 
      aagcaagtg atccactga atgtcaggt caaaaaccc 
      acttagaaa aatgtgcac agatgagga cattttttg 
      gtatcacgg aaaattgtc acgggaagc cacggaaaa 
      cctattaaa agaagaggg acgtcccga tgggagttt 
      tttagtgcg tgagagtaa ctcatcaac gggcgattt 
      cgtactctc agtccttca caacaatga agttagtca 
      ttttcaaat ccgtagaca cagcgaaga tgcgttttt 
      ttcaataga tgaaaatac taaaatcca cggccttga 
      gagtctgat agagcacta cgtaaacac aaaggggat 
      cctctcaga gggcctgtc tctgactgt tcccgtcaa 
      aggggacgc gccccccga ctattcacg cagacacgg 
      ccgcacgaa tttgctgca ccgcgccac aaaagaagg 
      gaattacaa agtcgtgtc tgaggttct taagaccgg 
      ctataggca cgaggcgaa gaaccagaa cggccaaac 
      ggccgtgca cttagccag catgaacgg caaggacga 
      cattctcag aaaactcat agactacgg tgctagtgt 
      taatttacg agatactgc cggatacac tcctcttca 
      ttatgcttg ccaaaacaa ttttccaag tactgtaag 
      actcctggt ccaagtggc caacgctaa catccagtc 
      ccggaacac cgaaactgg ggccgttcc tttacacga 
      agctgccag tcggggcca caaagacgt ggtcaaagc 
      attactaag cctaaatgc cccggttaa tccccggga 
      taaggataa ccaattgcc atcgcatct tgctaggaa 
      aaatgggtt catagaatg tgccgaaat tttagaaaa 
      ctatcaatg tccagcacc caaaacgca taggagtta 
      ctggtacca cggtaccct agaccgaca cgaagccga 
      aaccacgat caagcaatt cagtacgaa agacggcac 
      gtttttagt caggtggag cgaccgcaa caaagaacg 
      tgtcctcac ccttataaa cgagtcgtt gttttataa 
      ttacataat taggaaaca ggatgatta tttatttat 
      cgacaatgg gccttactt agaatcctt agagcatat 
      tattgaata ttatagttt tatccctga cggcttgcc 
      gacagtgtt acaaacacc tgtacctcc taaacctaa 
      gcccccggt ccctgaatg taccacaat acccagaca 
      acgaaagaa aaaccttag accgcaacc agaaataat 
      aagtatagc cgaatctca gtcgcttaa gtgttttcc 
      tagtcaatt tcaaaacat ccatttcac gacaaattt 
      ttcgaataa taataacta cacaacgaa cgacaacga 
      agatgacta tataccgct agaaaggct aaaaatggg 
      tcgggttat tggggaggg cgagttcgg ttcggtcta 
      cgaaggtac ttatacgaa acggaacgg agaggaaat 
      caaagtcgc tataaaaac agtcagaca cgagcaaat 
      cgaaacgag taatgccat tttgaagga agcccacgt 
      gatgatgag gctgaacca ccactgcgt ggttaagct 
      catcgggct gtcgaaagg gcgcccgct cctgatggt 
      gcaggaact agtcgcgct agggtctat gttgaacta 
      cattattct caataagga gagcattaa tccgaactc 
      ggagttcaa gatttgggc cgctcagat agcttgcgg 
      gatgaatta tttagaaga acaaagatt tattcatag 
      ggacctagc cgcaaggaa tatccttct agcgtccca 
      gcaccaggc aaaaatcag cgactttgg cctgtctag 
      agcgttggc cgctgatca tgaatatta cagagcttt 
      gcagggcgg gaaatggcc actgaagtg gtacgcccc 
      cgagtcgta caactacgg ccagttttc acacaaaag 
      tgatgtgtg gagtttcgg cgtcgccat ctgggaaat 
      gtacagttt cggtgacgt gccttacgg cgaattgaa 
      ggggtctga tgcgatcaa aattatcga agacggcga 
      gcggttgaa gcaaccgga cgcttgtcc ggaccatat 
      ttacgaaat aatgcggcg gtgttggga gtacgacgc 
      ggagaacag gccgacgtt caaggagct gttggagtt 
      tttctcgtc ggatacgga gtacatgaa catacggga 
      gttgctgca gagtgccaa cctggcctg aatttacgt 
      gccaattgt tttacatac tcataagtg caatataaa 
      gcatataaa ttg