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Gene information
General information
TC011608 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid67: 7473936..7482595
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011608
    iBeetle screen iB_02941
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    vitellarium shape irregular - Number of animals showing the phenotype: 3

    egg chamber segmentation/division decreased - Number of animals showing the phenotype: 3

    mature egg number increased - Number of animals showing the phenotype: 3
    Comment:
     
    potential held egg, but the division between the vitellogenic egg chambers does not seem to be normal.
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 87.5% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt not completely molted - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection



    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Pupal morphology
    wings orientation irregular - Number of animals showing the phenotype: 4
    wings blistered - Number of animals showing the phenotype: 1

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011608 RA
      MSGDREIPA EDSIKVVCR FRPLNDSEE KAGSKFIVK 
      FPSGGEDNC ISIAGKVYL FDKVFKPNA TQEKVYNEA 
      AKSIVSDVL AGYNGTIFA YGQTSSGKT HTMEGVIGD 
      PQKQGIIPR IVNDIFNHI YAMEENLEF HIKVSYFEI 
      YMDKIRDLL DVSKVNLSV HEDKNRVPY VKGATERFV 
      SSPEEVFES IEEGKSNRH IAVTNMNEH SSRSHSVFL 
      INVKQENLE NQKKLSGKL YLVDLAGSE KVSKTGAEG 
      TVLDEAKNI NKSLSALGN VISALADGN KTHIPYRDS 
      KLTRILQES LGGNARTTI VICCSPASF NESETKSTL 
      DFGKRAKTV KNVVCVNEE LTAEEWKRR YEKEKEKVA 
      RLKGKVEKL ESELQRWRA GETVKAEEQ VNLNELTEA 
      VTPVSGMEE STVEKQGPV PATPALMIG STLDVEERR 
      KLELERERL YQQLDEKDE EINEQSQYV EKLKEQMLE 
      QDELIASTR RDYEALQAE MNRIQQENE SAKEEVKEV 
      LQALEELAV NYDQKSQEV DAKNKEMET LSEELMQKQ 
      ASLNTTTTE LQQLRDMSS HQKKRIAEM LSNLLKELG 
      EIGSTLGSD GSEIKISND AAKLEEEFT VARLYISRM 
      KSEVTNLTQ RLHEIENKE QDSNKKVTE YEKELGECR 
      LLISQHEAR MKSLQESMR EAENKKRML EESVDALRE 
      ECAKLKAAE QVSSASESE KKEANELRL ALEQQMDQL 
      RVAHQKQVA ALRDEIAEK QQLINDIKD SNQKLSLAQ 
      QQLQSEYEK VKSEEAEKS QKLQELITT NERREQARK 
      DLRGLEDTV AKELQTLHN LRKLFVQDL QARMKKNLS 
      AEDNEEDGG SLAQKQKIS FLENNLDQL TKVHKQLVR 
      DNADLRCEL PKLEKRLRA TMERVKALE TALKEAKEG 
      AMRDRKRYQ YEVDRIKEA VRQKNLARR GPAATIAKP 
      IRSGQHHVV VGGGAAIRT GSNMGQKNM EIARKRESL 
      IINEKDACE S
    CDS sequence
    • >TC011608 RA
      atgtcgggc gatcgggaa attccagct gaagacagt 
      attaaagtc gtgtgtagg tttcgaccc ctcaatgat 
      tcagaggag aaggctggg tcgaaattc atcgtgaaa 
      ttcccttca ggaggcgaa gataattgt atttcgata 
      gcgggtaaa gtctactta ttcgacaag gtattcaaa 
      ccgaatgca actcaagaa aaagtgtac aatgaggcg 
      gccaagagc atcgtgtcc gatgttttg gccggttat 
      aacggcaca atcttcgcc tatggacaa acctcgtcg 
      ggtaaaacc cacacgatg gaaggcgta ataggtgat 
      ccacaaaaa cagggtata ataccccgc atagtcaac 
      gatatcttc aatcatatt tacgcaatg gaagagaat 
      cttgagttt cacatcaag gtttcgtat tttgaaatc 
      tacatggac aaaattagg gatttattg gacgtttcc 
      aaagtgaat ttatcggtc cacgaggac aaaaaccga 
      gttccttac gtgaaaggt gccaccgaa cggttcgtc 
      tcaagtcct gaggaggtt ttcgaatcg attgaagaa 
      ggcaaatcc aaccgacac atcgctgtg acaaacatg 
      aacgagcac tcgtctcga tcacactca gtatttttg 
      ataaacgtc aagcaagag aatctagaa aatcagaaa 
      aaactttcg gggaaattg tatttggtc gatttggct 
      ggttcggaa aaagtctcc aaaaccggg gctgaagga 
      acagtgcta gacgaagcc aaaaacatt aacaagtca 
      ctttcggcc cttggtaac gtgatcagt gctttagcc 
      gacggaaac aaaacacac ataccttac cgtgactct 
      aaactaaca agaatcctg caagaatcc ttgggcgga 
      aacgcccga acaacaatt gtgatttgt tgttctcca 
      gcgagtttc aacgaatcc gagacgaaa tcgacgctc 
      gatttcggc aaacgagcc aaaactgtt aaaaacgtc 
      gtctgtgtc aatgaggaa ctcacggcc gaagaatgg 
      aaacgtcgc tacgaaaaa gaaaaggaa aaagtggcg 
      cgtcttaaa ggcaaagtt gagaaactt gagagcgag 
      ttgcaacgt tggagggct ggagaaacg gtcaaagcc 
      gaggaacaa gtcaacttg aacgaacta accgaagct 
      gtgactcct gtctctgga atggaggaa agcacagtg 
      gagaaacag ggacctgtc ccggcaacc ccagccctc 
      atgatcggc tcaaccctc gatgtggaa gaacgcagg 
      aaactggag ctggaacgc gagcgactc taccaacaa 
      ctggacgaa aaagacgaa gagatcaac gagcaaagc 
      caatacgtt gagaaactc aaggagcag atgttggaa 
      caggacgag ttgattgca agcacgcga cgcgactac 
      gaagcgctc caagcggaa atgaaccga atccagcag 
      gagaatgag agtgccaag gaggaggtc aaggaagtc 
      ctgcaagcg cttgaagaa ctggccgtt aactacgac 
      caaaagtcg caagaagtg gacgccaag aacaaggag 
      atggaaacc ctgtccgag gagctgatg cagaagcaa 
      gccagcttg aataccacg accaccgaa ttgcagcaa 
      ttgcgggac atgtcgagt catcaaaag aagcgaatc 
      gccgagatg ttgagcaat ttgctgaag gagttgggc 
      gagattggg tcgactttg gggagtgac gggtccgag 
      attaagatc tccaatgat gcggctaag ctggaggag 
      gagtttacg gttgcacgt ttgtatatt tcgcggatg 
      aagagtgaa gttacgaat ttgacgcag cgattgcac 
      gaaattgaa aacaaggaa caggatagt aacaagaag 
      gtcacggag tatgagaag gagttgggg gagtgtagg 
      ttgttgatt tcgcaacac gaggccagg atgaagagt 
      ctacaagag tcgatgcgc gaagctgag aacaagaag 
      cggatgctg gaggagagt gttgatgcc ttgagggag 
      gagtgtgcg aagttgaag gccgctgaa caagtttcg 
      agcgccagc gagagcgag aagaaggaa gcgaacgag 
      ctgcgattg gctctcgag cagcaaatg gaccaatta 
      cgtgtcgct catcagaag caagtggcg gcgttacgc 
      gacgaaatc gccgaaaag cagcaattg atcaacgat 
      atcaaagat tcgaaccag aagttgtcg ctcgcccag 
      caacagttg cagtctgaa tacgagaaa gtcaagtcg 
      gaagaagcc gaaaagtcg cagaaattg caggagctc 
      attaccacg aacgaacga cgcgaacaa gcacggaaa 
      gacctccga ggattggaa gacaccgtc gccaaggag 
      ttgcaaacc cttcacaat ctccgaaaa ttattcgtc 
      caggactta caggcacgg atgaagaaa aatctctcg 
      gctgaagat aacgaagaa gacggcggt tcgttggcc 
      cagaaacag aaaatctcg tttttggag aataatttg 
      gaccagttg accaaagtg cataagcaa cttgtgaga 
      gataatgcc gatttgagg tgcgagttg cccaagctg 
      gagaagagg ctaagggcg accatggag agggtgaag 
      gcgctggag acggcgctc aaggaggcc aaggagggc 
      gccatgagg gatcgcaaa agatatcaa tacgaggtg 
      gaccggatc aaggaagcc gtccgccaa aagaacctc 
      gctcgtcgc ggccctgct gcaacaatt gccaagcct 
      atcaggtct ggccaacat catgttgtt gttggtggc 
      ggcgctgcg atccgtact gggtctaac atgggccag 
      aaaaacatg gaaattgcg cgaaaacgc gaatcactt 
      atcataaac gagaaagac gcatgtgag tcttga
    mRNA sequence
    • >TC011608 RA
      atgtaggag gagttgtca ttgttgcaa agaacccgc 
      aatttttta tacaaatgt atcatttat agttttact 
      tagtttgta attaattta ttaatttgt gctgtgagt 
      gattaaagt gctttacga tgtcgggcg atcgggaaa 
      ttccagctg aagacagta ttaaagtcg tgtgtaggt 
      ttcgacccc tcaatgatt cagaggaga aggctgggt 
      cgaaattca tcgtgaaat tcccttcag gaggcgaag 
      ataattgta tttcgatag cgggtaaag tctacttat 
      tcgacaagg tattcaaac cgaatgcaa ctcaagaaa 
      aagtgtaca atgaggcgg ccaagagca tcgtgtccg 
      atgttttgg ccggttata acggcacaa tcttcgcct 
      atggacaaa cctcgtcgg gtaaaaccc acacgatgg 
      aaggcgtaa taggtgatc cacaaaaac agggtataa 
      taccccgca tagtcaacg atatcttca atcatattt 
      acgcaatgg aagagaatc ttgagtttc acatcaagg 
      tttcgtatt ttgaaatct acatggaca aaattaggg 
      atttattgg acgtttcca aagtgaatt tatcggtcc 
      acgaggaca aaaaccgag ttccttacg tgaaaggtg 
      ccaccgaac ggttcgtct caagtcctg aggaggttt 
      tcgaatcga ttgaagaag gcaaatcca accgacaca 
      tcgctgtga caaacatga acgagcact cgtctcgat 
      cacactcag tatttttga taaacgtca agcaagaga 
      atctagaaa atcagaaaa aactttcgg ggaaattgt 
      atttggtcg atttggctg gttcggaaa aagtctcca 
      aaaccgggg ctgaaggaa cagtgctag acgaagcca 
      aaaacatta acaagtcac tttcggccc ttggtaacg 
      tgatcagtg ctttagccg acggaaaca aaacacaca 
      taccttacc gtgactcta aactaacaa gaatcctgc 
      aagaatcct tgggcggaa acgcccgaa caacaattg 
      tgatttgtt gttctccag cgagtttca acgaatccg 
      agacgaaat cgacgctcg atttcggca aacgagcca 
      aaactgtta aaaacgtcg tctgtgtca atgaggaac 
      tcacggccg aagaatgga aacgtcgct acgaaaaag 
      aaaaggaaa aagtggcgc gtcttaaag gcaaagttg 
      agaaacttg agagcgagt tgcaacgtt ggagggctg 
      gagaaacgg tcaaagccg aggaacaag tcaacttga 
      acgaactaa ccgaagctg tgactcctg tctctggaa 
      tggaggaaa gcacagtgg agaaacagg gacctgtcc 
      cggcaaccc cagccctca tgatcggct caaccctcg 
      atgtggaag aacgcagga aactggagc tggaacgcg 
      agcgactct accaacaac tggacgaaa aagacgaag 
      agatcaacg agcaaagcc aatacgttg agaaactca 
      aggagcaga tgttggaac aggacgagt tgattgcaa 
      gcacgcgac gcgactacg aagcgctcc aagcggaaa 
      tgaaccgaa tccagcagg agaatgaga gtgccaagg 
      aggaggtca aggaagtcc tgcaagcgc ttgaagaac 
      tggccgtta actacgacc aaaagtcgc aagaagtgg 
      acgccaaga acaaggaga tggaaaccc tgtccgagg 
      agctgatgc agaagcaag ccagcttga ataccacga 
      ccaccgaat tgcagcaat tgcgggaca tgtcgagtc 
      atcaaaaga agcgaatcg ccgagatgt tgagcaatt 
      tgctgaagg agttgggcg agattgggt cgactttgg 
      ggagtgacg ggtccgaga ttaagatct ccaatgatg 
      cggctaagc tggaggagg agtttacgg ttgcacgtt 
      tgtatattt cgcggatga agagtgaag ttacgaatt 
      tgacgcagc gattgcacg aaattgaaa acaaggaac 
      aggatagta acaagaagg tcacggagt atgagaagg 
      agttggggg agtgtaggt tgttgattt cgcaacacg 
      aggccagga tgaagagtc tacaagagt cgatgcgcg 
      aagctgaga acaagaagc ggatgctgg aggagagtg 
      ttgatgcct tgagggagg agtgtgcga agttgaagg 
      ccgctgaac aagtttcga gcgccagcg agagcgaga 
      agaaggaag cgaacgagc tgcgattgg ctctcgagc 
      agcaaatgg accaattac gtgtcgctc atcagaagc 
      aagtggcgg cgttacgcg acgaaatcg ccgaaaagc 
      agcaattga tcaacgata tcaaagatt cgaaccaga 
      agttgtcgc tcgcccagc aacagttgc agtctgaat 
      acgagaaag tcaagtcgg aagaagccg aaaagtcgc 
      agaaattgc aggagctca ttaccacga acgaacgac 
      gcgaacaag cacggaaag acctccgag gattggaag 
      acaccgtcg ccaaggagt tgcaaaccc ttcacaatc 
      tccgaaaat tattcgtcc aggacttac aggcacgga 
      tgaagaaaa atctctcgg ctgaagata acgaagaag 
      acggcggtt cgttggccc agaaacaga aaatctcgt 
      ttttggaga ataatttgg accagttga ccaaagtgc 
      ataagcaac ttgtgagag ataatgccg atttgaggt 
      gcgagttgc ccaagctgg agaagaggc taagggcga 
      ccatggaga gggtgaagg cgctggaga cggcgctca 
      aggaggcca aggagggcg ccatgaggg atcgcaaaa 
      gatatcaat acgaggtgg accggatca aggaagccg 
      tccgccaaa agaacctcg ctcgtcgcg gccctgctg 
      caacaattg ccaagccta tcaggtctg gccaacatc 
      atgttgttg ttggtggcg gcgctgcga tccgtactg 
      ggtctaaca tgggccaga aaaacatgg aaattgcgc 
      gaaaacgcg aatcactta tcataaacg agaaagacg 
      catgtgagt cttgattta atttttttc tctaatttc 
      ctgaatttt tccttccag aaacggtgc tctgatcac 
      tgggatacc aaaaatatt gctgactac tcgtactac 
      gtaagccgt ggaacaaca cacgactta catttttgt 
      gcttacttg cttagttat cctagaatt aaggtgcac 
      ttattgaga cgacaaatt gtttcttag gtcggtttt 
      tagtgcaat tgctaagaa ataatttgt gggttttcc 
      tgtcgcatg tcgcgcgct attataatc atggtggtg 
      taataacga ttcaaattt tccaggctt aaatcgtgt 
      tttgtaata taaaagtgg cattatttt aggatttta 
      gttgtctct cctcttgat caaatggtc taactagca 
      cgtttaggt aattaagta gcacatttt acacaactg 
      gattatttg tatagcctg attgatgta ttttgtata 
      aatttgttt acgattgat tgattgctt gtagtggtc 
      taagtatat attttttta aaacgattt tattttgtt 
      gtttttctc aatggtgca ggtcggacg gttggcttg 
      acgggcgca agtgctaat cgctccgat gcaatctaa 
      tttcattgt gataagtag caaagtttt gttcttccg 
      tcaaagacc cgattggtg cacaattgc atttctaat 
      ttatttgtt tttgttgac tgtagataa ttgtttagt 
      aacagtctt tggtacagc tgtcgtagt tgtgtaacg 
      aaactgata gtggatgct tgagtcaat aatgcaatt 
      tatttattt aaatgatgg aaattatat tctaataaa 
      ctaatacaa atttc