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Gene information
General information
TC012837 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15784527..15786281
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012837
    iBeetle screen iB_02940

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012837 RA
      MSKYVLVLV ICSIPFSAC MRKNVCKTI EDYRTIEHN 
      PNCWYNPDV GASPAEIIK GHGYPFESY EVVTKDGYI 
      VTLFRVPHN GTNFGAKKP VVFLQHGMA VDSSCYLYL 
      GEKSSVFVF ANNGYDVWL SNSRGTKYS SKHNKYSVY 
      DPAYWNFSF HEMAIYDLP AMLEFIIKM TGQTGHIHY 
      IGHSMGTTI SYIYSSLMP QHAKRSLRT IVSLAPVAF 
      LDHVSPLVR AIVPFRYLI WDFFASLGL YGVGPNVQF 
      KLDIVLQMC ARYPFILIC SNLGDLAGT NQAENRAST 
      LPVSAAAQL SGISLKTLL HYAQIIDAR GRFQYFDYG 
      PYNRKIYNS TLPPEYPIY KIKIPVYLF YGRRDLLAT 
      EKDVYHLYE KLKTEKRIM EVFNFGHNN FLSGLHALD 
      LYQMILKLI Q
    CDS sequence
    • >TC012837 RA
      atgtccaag tatgtgcta gtgctcgta atttgctca 
      ataccattt agtgcatgt atgcgtaaa aatgtttgc 
      aaaacgatc gaggattac aggacaata gagcacaat 
      ccgaattgt tggtacaat cccgacgtc ggagccagt 
      ccggccgaa attatcaaa ggacacggt tatcctttt 
      gaaagctat gaagttgtg acaaaggac ggctacatt 
      gttacgtta tttcgggtg cctcataac ggcaccaat 
      ttcggggcg aaaaaaccg gtggttttc ctccagcat 
      ggaatggct gttgactct tcgtgttat ctctatttg 
      ggcgaaaag tccagcgtt tttgttttc gcgaataat 
      ggatacgac gtctggttg tccaacagc agaggcacc 
      aaatactcc tcaaaacac aacaaatat tcagtttat 
      gaccccgca tactggaat ttcagtttt cacgaaatg 
      gcaatttat gaccttcca gccatgctg gaatttatt 
      attaaaatg accggacaa actggacat atccactac 
      atcggccat tctatggga accacaatt tcgtacatt 
      tacagctct ctgatgcca caacatgcc aaaaggtcc 
      ttaaggaca attgtgtct ctcgcacct gtggctttt 
      ctggaccat gtctcacct ctagtgcgt gccattgtc 
      ccatttcgc tacttaatt tgggatttt ttcgcctcg 
      cttggatta tatggagtt ggtccgaac gtgcaattt 
      aagctagat atagtcctg caaatgtgc gcccgctat 
      ccgtttatt ttgatttgt tcgaatttg ggcgatctt 
      gccggaacg aatcaggcg gagaacagg gcgagcact 
      cttcctgtc agtgcagcg gcccaattg tcgggaatt 
      tcgctaaaa acgctccta cattacgcc caaataatt 
      gacgcgagg ggccgcttt caatatttt gattacggt 
      ccgtataat cggaaaatt tataactcg acattgccg 
      cccgaatat ccaatttat aaaattaaa atccctgtt 
      tatttattt tatgggcga agagattta ctagccacg 
      gaaaaggat gtttatcat ttgtacgag aaattgaag 
      actgaaaaa aggataatg gaggttttc aatttcggg 
      cacaataat tttcttagc ggtttacac gcgttggat 
      ttgtaccaa atgatactc aagttgatt cagtga
    mRNA sequence
    • >TC012837 RA
      ttctccgtc aaggataac gcacgcaaa gtataaata 
      aaggattat ttacaataa atatcctca gtttagcgc 
      atggtataa aaatgtcca agtatgtgc tagtgctcg 
      taatttgct caataccat ttagtgcat gtatgcgta 
      aaaatgttt gcaaaacga tcgaggatt acaggacaa 
      tagagcaca atccgaatt gttggtaca atcccgacg 
      tcggagcca gtccggccg aaattatca aaggacacg 
      gttatcctt ttgaaagct atgaagttg tgacaaagg 
      acggctaca ttgttacgt tatttcggg tgcctcata 
      acggcacca atttcgggg cgaaaaaac cggtggttt 
      tcctccagc atggaatgg ctgttgact cttcgtgtt 
      atctctatt tgggcgaaa agtccagcg tttttgttt 
      tcgcgaata atggatacg acgtctggt tgtccaaca 
      gcagaggca ccaaatact cctcaaaac acaacaaat 
      attcagttt atgaccccg catactgga atttcagtt 
      ttcacgaaa tggcaattt atgaccttc cagccatgc 
      tggaattta ttattaaaa tgaccggac aaactggac 
      atatccact acatcggcc attctatgg gaaccacaa 
      tttcgtaca tttacagct ctctgatgc cacaacatg 
      ccaaaaggt ccttaagga caattgtgt ctctcgcac 
      ctgtggctt ttctggacc atgtctcac ctctagtgc 
      gtgccattg tcccatttc gctacttaa tttgggatt 
      ttttcgcct cgcttggat tatatggag ttggtccga 
      acgtgcaat ttaagctag atatagtcc tgcaaatgt 
      gcgcccgct atccgttta ttttgattt gttcgaatt 
      tgggcgatc ttgccggaa cgaatcagg cggagaaca 
      gggcgagca ctcttcctg tcagtgcag cggcccaat 
      tgtcgggaa tttcgctaa aaacgctcc tacattacg 
      cccaaataa ttgacgcga ggggccgct ttcaatatt 
      ttgattacg gtccgtata atcggaaaa tttataact 
      cgacattgc cgcccgaat atccaattt ataaaatta 
      aaatccctg tttatttat tttatgggc gaagagatt 
      tactagcca cggaaaagg atgtttatc atttgtacg 
      agaaattga agactgaaa aaaggataa tggaggttt 
      tcaatttcg ggcacaata attttctta gcggtttac 
      acgcgttgg atttgtacc aaatgatac tcaagttga 
      ttcagtgat gccaaacga ttaattaat ttatttatt 
      taataaacg attttacac aaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY