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Gene information
General information
TC012830 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid67: 8058137..8060960
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012830
    iBeetle screen iB_02939
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle random remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    head shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: there are many different phenotypes on the slide
    head partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012830 RA
      MSEKTQTEP RKVAQFLAT TLATLSALA MGLCLTWTS 
      PALPMLEQP TTYPKITKN QGAWIGSLL TLGAFCGAI 
      PAGTLANFI GRKRSLLFF ALPLFISWI IIAYGNCVG 
      VLYFARFLA GLAIGAISV AAPMYVTEI AHTSIRGTL 
      GTFFQVQIT VGVLVGYIL GTTIESFQY LALVSSVFP 
      LLFVSGFAF MPETPAYLY ATGRIDAAR KSLIFFRGR 
      DYNLLDEEL QKIAEDIKE STANKPKLS DLIRNRVTL 
      NGLVVSLGL MAFQQLSGV NAVLFYAGN IFAETGNSM 
      GADTCAVLV GAVQVIATL LSTVLIDKT GRKILLLVS 
      SSIMCLSLL ALGLYFFLK QTQDLSFLS ALPLVSLAV 
      FIVVFSIGM GPIPWLMMG EIFTPKSKG VATSVSAAF 
      NWVMAFTVT NQYQNLNEM LGVGGTFMA FGGICALGV 
      LFIALLVPE TKGKDIDQV QEALMRTSR V
    CDS sequence
    • >TC012830 RA
      atgagtgaa aaaacacaa actgaacca cgaaaagtg 
      gcccaattc ctcgccaca acattagca actttaagc 
      gctcttgca atgggattg tgtcttacg tggacttca 
      ccagccctt cctatgctc gaacaaccc acaacctat 
      ccaaaaatt acaaaaaat cagggcgct tggattgga 
      tctttattg actttgggt gcgttttgt ggcgctatt 
      cctgcggga actttggcc aattttatc ggacgtaaa 
      cggagttta ttatttttt gcactgcct ctgtttatt 
      tcgtggatt attatagcc tatgggaat tgtgttggg 
      gttttatac tttgcacgg tttctggca gggttggca 
      ataggggcg ataagtgtc gccgcccct atgtatgtg 
      actgaaata gcgcatacc tcaattagg ggtacttta 
      gggactttt tttcaagta cagatcact gtgggagta 
      ttagttggt tacattcta ggaacaaca atcgagtct 
      ttccagtat ttggcattg gtgtcgtca gtatttccc 
      ctcctgttt gtttctggt tttgctttt atgcctgaa 
      acaccggct tatttgtac gccactggt cgaatcgac 
      gcagctcgt aaatcacta attttcttc agaggacgt 
      gattacaac cttctcgac gaagaatta cagaaaatt 
      gccgaagat attaaagaa tcgacggca aataagccg 
      aaattgtcg gatcttatc cgaaacaga gtaactttg 
      aatggactt gtggtgtct ttaggattg atggctttc 
      caacaattg agtggagtc aatgcggtg cttttttat 
      gctggaaat attttcgct gaaacagga aattcgatg 
      ggagccgat acgtgtgcg gttttagtg ggcgctgtt 
      caggtgatc gccactctg ctatcgacc gtcctcatc 
      gacaaaaca ggtcgcaag atcctgctg ctcgtctcc 
      tcgtccatt atgtgcctt tcgttgctt gccctcggg 
      ctctacttc ttcctgaaa cagacgcaa gacttgtct 
      tttctcagc gccttgccg ctggtcagt ttggccgtg 
      tttatcgtc gtcttttcc attggcatg ggcccaata 
      ccctggctg atgatgggc gaaattttc acacctaaa 
      tcgaaggga gtggcgact tcggtctct gcggcgttt 
      aattgggtt atggcattt acggtcacc aatcagtac 
      cagaatttg aacgaaatg ctgggagta ggaggcact 
      tttatggcc tttggggga atttgcgct ctaggagtg 
      ctttttatc gcgctttta gtacccgag actaaagga 
      aaggatatc gatcaagtg caagaagct ctaatgcgt 
      acgagtcga gtttag
    mRNA sequence
    • >TC012830 RA
      atgagtgaa aaaacacaa actgaacca cgaaaagtg 
      gcccaattc ctcgccaca acattagca actttaagc 
      gctcttgca atgggattg tgtcttacg tggacttca 
      ccagccctt cctatgctc gaacaaccc acaacctat 
      ccaaaaatt acaaaaaat cagggcgct tggattgga 
      tctttattg actttgggt gcgttttgt ggcgctatt 
      cctgcggga actttggcc aattttatc ggacgtaaa 
      cggagttta ttatttttt gcactgcct ctgtttatt 
      tcgtggatt attatagcc tatgggaat tgtgttggg 
      gttttatac tttgcacgg tttctggca gggttggca 
      ataggggcg ataagtgtc gccgcccct atgtatgtg 
      actgaaata gcgcatacc tcaattagg ggtacttta 
      gggactttt tttcaagta cagatcact gtgggagta 
      ttagttggt tacattcta ggaacaaca atcgagtct 
      ttccagtat ttggcattg gtgtcgtca gtatttccc 
      ctcctgttt gtttctggt tttgctttt atgcctgaa 
      acaccggct tatttgtac gccactggt cgaatcgac 
      gcagctcgt aaatcacta attttcttc agaggacgt 
      gattacaac cttctcgac gaagaatta cagaaaatt 
      gccgaagat attaaagaa tcgacggca aataagccg 
      aaattgtcg gatcttatc cgaaacaga gtaactttg 
      aatggactt gtggtgtct ttaggattg atggctttc 
      caacaattg agtggagtc aatgcggtg cttttttat 
      gctggaaat attttcgct gaaacagga aattcgatg 
      ggagccgat acgtgtgcg gttttagtg ggcgctgtt 
      caggtgatc gccactctg ctatcgacc gtcctcatc 
      gacaaaaca ggtcgcaag atcctgctg ctcgtctcc 
      tcgtccatt atgtgcctt tcgttgctt gccctcggg 
      ctctacttc ttcctgaaa cagacgcaa gacttgtct 
      tttctcagc gccttgccg ctggtcagt ttggccgtg 
      tttatcgtc gtcttttcc attggcatg ggcccaata 
      ccctggctg atgatgggc gaaattttc acacctaaa 
      tcgaaggga gtggcgact tcggtctct gcggcgttt 
      aattgggtt atggcattt acggtcacc aatcagtac 
      cagaatttg aacgaaatg ctgggagta ggaggcact 
      tttatggcc tttggggga atttgcgct ctaggagtg 
      ctttttatc gcgctttta gtacccgag actaaagga 
      aaggatatc gatcaagtg caagaagct ctaatgcgt 
      acgagtcga gtttag