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Gene information
General information
TC012818 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15994988..15998305
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012818
    iBeetle screen iB_02938

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012818 RA
      MKILSQNVI WLLIFVAYN YLFCLADDK NKITVGAFF 
EREDVQSKA ALNYAIDTT NMMQQHLKY ALKTQILAQ 
NDSFYCCKL LCNVTQEGS GLAAIFASK PIFESLSNR 
LEIPFILTK WRPASYSNK QTTVNFFPD SYLFSHGLA 
IIVKNLQWK NFVLLYDSD KGLVKLQQI LKLNNFNSG 
SVIVRQLGP GPDHRPLLK EIRALNHNR IILDCDTEN 
IIEILKQAK EVNLMESSY FNYFLTSVD AHTLDFSVL 
NTTANITTI RILDFTQTQ SNYSTRTET ALIQDGIHS 
FITSVNTLH VTEPIVPSP MACDQKWSH GFRISSFMR 
VREFFGASG PIGFDSSGR RLNFTIFVV EGNRENVVA 
KWRPENPEI LIYMRGEND SFDALVKNM QKSVLIISS 
RLGPPYLME RKPRFEGEI LTGNSRYEG FSMDLIDAI 
AGILGFKYE FRLAKDGKY GNYDPETKS WNGLIKDLL 
DRKADLAIC DLTITHQRR EVVDFSMPF MRLGISILY 
KKAEEKDVN IFAFLEPFS PEIWIYTAT LYLVVSVIL 
YLVARMAPG DWENPHACN PKPEKLENI WNLKNCLWL 
TLGSIMTQG CDILPKGIS SRLATSMWW FFSLIMTSS 
YTANLAAFL TMERLEPTI DSAEALAKQ TKIKYGTVE 
GGATQAFFR ESNYSTYQK MWTTMIQAK PGVFEKNNA 
DGVKRVQTT KNRLYAFLM ESSQIEYEI ETKCDLKQV 
GNWLDNKEY GIAMPIDYP YRSAINTAI LKLQEEAKL 
TELKDKWWK KMRDEPSCP VRTLGKSST ELALDNVGG 
VFLVLGVGM AVAFVLAIL EFLWNVRNI SVEEHMTYF 
EALKVELIF ALNVWVTKK RTKPKINSL NCVLRTE
    CDS sequence
    • >TC012818 RA
      atgaaaatt ctctcccaa aacgtaatt tggctctta 
attttcgtc gcttacaat tatctcttc tgtctcgct 
gatgacaaa aataaaata acagtcggt gcatttttt 
gaacgtgaa gacgtccaa tctaaagca gccctcaac 
tatgccata gataccaca aatatgatg caacaacat 
ttgaaatat gccctcaaa acccaaatt ttagcgcag 
aatgattcg ttttactgt tgcaaactt ttatgtaat 
gtgacgcaa gaagggtcg ggattggct gcaattttc 
gcctcaaaa cctattttc gaatcatta agtaatcga 
ctagaaatt ccgttcatc ttgacaaaa tggcgcccc 
gcctcttat tcaaataaa caaacgacg gtcaacttc 
ttccccgat tcgtatttg ttttcgcac ggtctagcc 
atcattgtt aaaaatttg caatggaaa aattttgtc 
ttactttac gacagcgac aaaggtctt gtaaagttg 
caacaaatt ttaaaattg aataatttc aattccggt 
tctgtgatt gtaaggcag ttggggcca ggaccggat 
caccgtcct ttattgaaa gaaattagg gctttgaat 
cacaaccgg attattcta gactgcgac actgaaaac 
atcatcgaa attttgaag caagccaaa gaagtcaat 
ttgatggaa tcgtcatac tttaattac tttctaacg 
tctgtggac gcacacact ttggatttt tccgtcttg 
aacactact gccaacatt acaacaatc cgaattttg 
gatttcaca caaacccaa tcaaattac tcgactcgg 
accgaaacg gctctcatt caagacggt attcattca 
tttatcact tctgtgaat actctccac gtgaccgaa 
ccaattgta ccatcacca atggcgtgc gatcaaaaa 
tggtcacac gggttcagg attagttcg ttcatgaga 
gtcagagaa ttttttggt gctagtggc cctattggg 
tttgattca tcaggtcgt aggctcaat ttcacgatt 
tttgttgtt gaaggcaat cgggaaaat gttgtagct 
aagtggaga cccgaaaat ccagaaatt ttgatctac 
atgcggggg gaaaatgat agttttgat gcgttggtt 
aaaaacatg caaaagagt gttttgata atctcttca 
agactcggt cctccatat ttaatggaa cgcaagcct 
cgtttcgag ggtgaaatt ctgacaggg aactcccgt 
tacgagggt ttttccatg gacctcata gatgccata 
gccgggatt ttaggtttt aaatacgaa tttcgtttg 
gcaaaagac ggcaaatat ggcaattat gaccccgag 
accaaatct tggaacggc ctgattaaa gatttattg 
gatcgaaaa gctgatttg gcaatttgc gacctaact 
atcactcat caaaggcga gaagtggtc gattttagt 
atgccattt atgcgactg gggataagt attttgtac 
aaaaaagcc gaagagaaa gatgtcaac atttttgct 
tttttggag cctttctcg cccgaaatt tggatttac 
acggccact ttgtatcta gttgtttca gtgattttg 
tatttggta gcaaggatg gcccccgga gattgggaa 
aacccacac gcttgcaac cccaaacct gaaaaactt 
gaaaatatt tggaacctc aaaaattgc ttgtggttg 
actttgggg tccataatg acccaaggt tgcgacatt 
ttaccaaag ggaatttca tcacgactg gcaacttca 
atgtggtgg ttcttttcc ctaatcatg acaagttca 
tacacagcc aacttggcc gcttttctc acaatggaa 
cgcctggag ccaaccata gacagcgcc gaggcttta 
gccaaacaa acgaaaatc aaatacgga acagttgaa 
ggtggagca acccaagcc ttctttcgc gaatcgaat 
tactcgacg tatcagaaa atgtggact actatgatt 
caagcaaaa ccgggcgtt tttgaaaaa aataacgca 
gatggcgtg aaacgagtc caaacaacc aagaatcgc 
ctctacgcc ttcctgatg gaatcgtca cagattgag 
tacgagatc gagacgaaa tgcgatttg aaacaagtc 
ggcaattgg ttggataac aaggagtat gggattgct 
atgcccatt gattatcca tacagaagt gccataaat 
accgcaatt ttgaaacta caagaggaa gcaaaattg 
accgaactg aaggacaaa tggtggaaa aaaatgcgc 
gatgagccc tcttgtccc gtgcgaaca ctaggaaaa 
agttccaca gagttggcg ttggataat gttgggggc 
gtttttttg gtactgggg gtaggaatg gcggttgct 
tttgtttta gcaatcctc gagtttttg tggaacgtt 
cgaaatatt tcagtggag gaacacatg acctatttt 
gaagcactc aaggtcgaa ttaatattc gctttaaat 
gtttgggtc acgaaaaag agaaccaaa ccgaaaatt 
aattcgcta aactgcgtt ttgcgcacc gagtaa
    mRNA sequence
    • >TC012818 RA
      cgtgtcttt gatttatca cacaacaat gaaaattct 
ctcccaaaa cgtaatttg gctcttaat tttcgtcgc 
ttacaatta tctcttctg tctcgctga tgacaaaaa 
taaaataac agtcggtgc attttttga acgtgaaga 
cgtccaatc taaagcagc cctcaacta tgccataga 
taccacaaa tatgatgca acaacattt gaaatatgc 
cctcaaaac ccaaatttt agcgcagaa tgattcgtt 
ttactgttg caaactttt atgtaatgt gacgcaaga 
agggtcggg attggctgc aattttcgc ctcaaaacc 
tattttcga atcattaag taatcgact agaaattcc 
gttcatctt gacaaaatg gcgccccgc ctcttattc 
aaataaaca aacgacggt caacttctt ccccgattc 
gtatttgtt ttcgcacgg tctagccat cattgttaa 
aaatttgca atggaaaaa ttttgtctt actttacga 
cagcgacaa aggtcttgt aaagttgca acaaatttt 
aaaattgaa taatttcaa ttccggttc tgtgattgt 
aaggcagtt ggggccagg accggatca ccgtccttt 
attgaaaga aattagggc tttgaatca caaccggat 
tattctaga ctgcgacac tgaaaacat catcgaaat 
tttgaagca agccaaaga agtcaattt gatggaatc 
gtcatactt taattactt tctaacgtc tgtggacgc 
acacacttt ggatttttc cgtcttgaa cactactgc 
caacattac aacaatccg aattttgga tttcacaca 
aacccaatc aaattactc gactcggac cgaaacggc 
tctcattca agacggtat tcattcatt tatcacttc 
tgtgaatac tctccacgt gaccgaacc aattgtacc 
atcaccaat ggcgtgcga tcaaaaatg gtcacacgg 
gttcaggat tagttcgtt catgagagt cagagaatt 
ttttggtgc tagtggccc tattgggtt tgattcatc 
aggtcgtag gctcaattt cacgatttt tgttgttga 
aggcaatcg ggaaaatgt tgtagctaa gtggagacc 
cgaaaatcc agaaatttt gatctacat gcgggggga 
aaatgatag ttttgatgc gttggttaa aaacatgca 
aaagagtgt tttgataat ctcttcaag actcggtcc 
tccatattt aatggaacg caagcctcg tttcgaggg 
tgaaattct gacagggaa ctcccgtta cgagggttt 
ttccatgga cctcataga tgccatagc cgggatttt 
aggttttaa atacgaatt tcgtttggc aaaagacgg 
caaatatgg caattatga ccccgagac caaatcttg 
gaacggcct gattaaaga tttattgga tcgaaaagc 
tgatttggc aatttgcga cctaactat cactcatca 
aaggcgaga agtggtcga ttttagtat gccatttat 
gcgactggg gataagtat tttgtacaa aaaagccga 
agagaaaga tgtcaacat ttttgcttt tttggagcc 
tttctcgcc cgaaatttg gatttacac ggccacttt 
gtatctagt tgtttcagt gattttgta tttggtagc 
aaggatggc ccccggaga ttgggaaaa cccacacgc 
ttgcaaccc caaacctga aaaacttga aaatatttg 
gaacctcaa aaattgctt gtggttgac tttggggtc 
cataatgac ccaaggttg cgacatttt accaaaggg 
aatttcatc acgactggc aacttcaat gtggtggtt 
cttttccct aatcatgac aagttcata cacagccaa 
cttggccgc ttttctcac aatggaacg cctggagcc 
aaccataga cagcgccga ggctttagc caaacaaac 
gaaaatcaa atacggaac agttgaagg tggagcaac 
ccaagcctt ctttcgcga atcgaatta ctcgacgta 
tcagaaaat gtggactac tatgattca agcaaaacc 
gggcgtttt tgaaaaaaa taacgcaga tggcgtgaa 
acgagtcca aacaaccaa gaatcgcct ctacgcctt 
cctgatgga atcgtcaca gattgagta cgagatcga 
gacgaaatg cgatttgaa acaagtcgg caattggtt 
ggataacaa ggagtatgg gattgctat gcccattga 
ttatccata cagaagtgc cataaatac cgcaatttt 
gaaactaca agaggaagc aaaattgac cgaactgaa 
ggacaaatg gtggaaaaa aatgcgcga tgagccctc 
ttgtcccgt gcgaacact aggaaaaag ttccacaga 
gttggcgtt ggataatgt tgggggcgt ttttttggt 
actgggggt aggaatggc ggttgcttt tgttttagc 
aatcctcga gtttttgtg gaacgttcg aaatatttc 
agtggagga acacatgac ctattttga agcactcaa 
ggtcgaatt aatattcgc tttaaatgt ttgggtcac 
gaaaaagag aaccaaacc gaaaattaa ttcgctaaa 
ctgcgtttt gcgcaccga gtaataaaa aaccaaact 
agaaaatcg tgtttatta atcaaaaat attgttttc 
ataaataat atgaataaa tatgacaag aaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY