| General information | |||
| TC012818 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 15994988..15998305 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC012818 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02938 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
- >TC012818 RA
MKILSQNVI WLLIFVAYN YLFCLADDK NKITVGAFF EREDVQSKA ALNYAIDTT NMMQQHLKY ALKTQILAQ NDSFYCCKL LCNVTQEGS GLAAIFASK PIFESLSNR LEIPFILTK WRPASYSNK QTTVNFFPD SYLFSHGLA IIVKNLQWK NFVLLYDSD KGLVKLQQI LKLNNFNSG SVIVRQLGP GPDHRPLLK EIRALNHNR IILDCDTEN IIEILKQAK EVNLMESSY FNYFLTSVD AHTLDFSVL NTTANITTI RILDFTQTQ SNYSTRTET ALIQDGIHS FITSVNTLH VTEPIVPSP MACDQKWSH GFRISSFMR VREFFGASG PIGFDSSGR RLNFTIFVV EGNRENVVA KWRPENPEI LIYMRGEND SFDALVKNM QKSVLIISS RLGPPYLME RKPRFEGEI LTGNSRYEG FSMDLIDAI AGILGFKYE FRLAKDGKY GNYDPETKS WNGLIKDLL DRKADLAIC DLTITHQRR EVVDFSMPF MRLGISILY KKAEEKDVN IFAFLEPFS PEIWIYTAT LYLVVSVIL YLVARMAPG DWENPHACN PKPEKLENI WNLKNCLWL TLGSIMTQG CDILPKGIS SRLATSMWW FFSLIMTSS YTANLAAFL TMERLEPTI DSAEALAKQ TKIKYGTVE GGATQAFFR ESNYSTYQK MWTTMIQAK PGVFEKNNA DGVKRVQTT KNRLYAFLM ESSQIEYEI ETKCDLKQV GNWLDNKEY GIAMPIDYP YRSAINTAI LKLQEEAKL TELKDKWWK KMRDEPSCP VRTLGKSST ELALDNVGG VFLVLGVGM AVAFVLAIL EFLWNVRNI SVEEHMTYF EALKVELIF ALNVWVTKK RTKPKINSL NCVLRTE
- >TC012818 RA
atgaaaatt ctctcccaa aacgtaatt tggctctta attttcgtc gcttacaat tatctcttc tgtctcgct gatgacaaa aataaaata acagtcggt gcatttttt gaacgtgaa gacgtccaa tctaaagca gccctcaac tatgccata gataccaca aatatgatg caacaacat ttgaaatat gccctcaaa acccaaatt ttagcgcag aatgattcg ttttactgt tgcaaactt ttatgtaat gtgacgcaa gaagggtcg ggattggct gcaattttc gcctcaaaa cctattttc gaatcatta agtaatcga ctagaaatt ccgttcatc ttgacaaaa tggcgcccc gcctcttat tcaaataaa caaacgacg gtcaacttc ttccccgat tcgtatttg ttttcgcac ggtctagcc atcattgtt aaaaatttg caatggaaa aattttgtc ttactttac gacagcgac aaaggtctt gtaaagttg caacaaatt ttaaaattg aataatttc aattccggt tctgtgatt gtaaggcag ttggggcca ggaccggat caccgtcct ttattgaaa gaaattagg gctttgaat cacaaccgg attattcta gactgcgac actgaaaac atcatcgaa attttgaag caagccaaa gaagtcaat ttgatggaa tcgtcatac tttaattac tttctaacg tctgtggac gcacacact ttggatttt tccgtcttg aacactact gccaacatt acaacaatc cgaattttg gatttcaca caaacccaa tcaaattac tcgactcgg accgaaacg gctctcatt caagacggt attcattca tttatcact tctgtgaat actctccac gtgaccgaa ccaattgta ccatcacca atggcgtgc gatcaaaaa tggtcacac gggttcagg attagttcg ttcatgaga gtcagagaa ttttttggt gctagtggc cctattggg tttgattca tcaggtcgt aggctcaat ttcacgatt tttgttgtt gaaggcaat cgggaaaat gttgtagct aagtggaga cccgaaaat ccagaaatt ttgatctac atgcggggg gaaaatgat agttttgat gcgttggtt aaaaacatg caaaagagt gttttgata atctcttca agactcggt cctccatat ttaatggaa cgcaagcct cgtttcgag ggtgaaatt ctgacaggg aactcccgt tacgagggt ttttccatg gacctcata gatgccata gccgggatt ttaggtttt aaatacgaa tttcgtttg gcaaaagac ggcaaatat ggcaattat gaccccgag accaaatct tggaacggc ctgattaaa gatttattg gatcgaaaa gctgatttg gcaatttgc gacctaact atcactcat caaaggcga gaagtggtc gattttagt atgccattt atgcgactg gggataagt attttgtac aaaaaagcc gaagagaaa gatgtcaac atttttgct tttttggag cctttctcg cccgaaatt tggatttac acggccact ttgtatcta gttgtttca gtgattttg tatttggta gcaaggatg gcccccgga gattgggaa aacccacac gcttgcaac cccaaacct gaaaaactt gaaaatatt tggaacctc aaaaattgc ttgtggttg actttgggg tccataatg acccaaggt tgcgacatt ttaccaaag ggaatttca tcacgactg gcaacttca atgtggtgg ttcttttcc ctaatcatg acaagttca tacacagcc aacttggcc gcttttctc acaatggaa cgcctggag ccaaccata gacagcgcc gaggcttta gccaaacaa acgaaaatc aaatacgga acagttgaa ggtggagca acccaagcc ttctttcgc gaatcgaat tactcgacg tatcagaaa atgtggact actatgatt caagcaaaa ccgggcgtt tttgaaaaa aataacgca gatggcgtg aaacgagtc caaacaacc aagaatcgc ctctacgcc ttcctgatg gaatcgtca cagattgag tacgagatc gagacgaaa tgcgatttg aaacaagtc ggcaattgg ttggataac aaggagtat gggattgct atgcccatt gattatcca tacagaagt gccataaat accgcaatt ttgaaacta caagaggaa gcaaaattg accgaactg aaggacaaa tggtggaaa aaaatgcgc gatgagccc tcttgtccc gtgcgaaca ctaggaaaa agttccaca gagttggcg ttggataat gttgggggc gtttttttg gtactgggg gtaggaatg gcggttgct tttgtttta gcaatcctc gagtttttg tggaacgtt cgaaatatt tcagtggag gaacacatg acctatttt gaagcactc aaggtcgaa ttaatattc gctttaaat gtttgggtc acgaaaaag agaaccaaa ccgaaaatt aattcgcta aactgcgtt ttgcgcacc gagtaa
- >TC012818 RA
cgtgtcttt gatttatca cacaacaat gaaaattct ctcccaaaa cgtaatttg gctcttaat tttcgtcgc ttacaatta tctcttctg tctcgctga tgacaaaaa taaaataac agtcggtgc attttttga acgtgaaga cgtccaatc taaagcagc cctcaacta tgccataga taccacaaa tatgatgca acaacattt gaaatatgc cctcaaaac ccaaatttt agcgcagaa tgattcgtt ttactgttg caaactttt atgtaatgt gacgcaaga agggtcggg attggctgc aattttcgc ctcaaaacc tattttcga atcattaag taatcgact agaaattcc gttcatctt gacaaaatg gcgccccgc ctcttattc aaataaaca aacgacggt caacttctt ccccgattc gtatttgtt ttcgcacgg tctagccat cattgttaa aaatttgca atggaaaaa ttttgtctt actttacga cagcgacaa aggtcttgt aaagttgca acaaatttt aaaattgaa taatttcaa ttccggttc tgtgattgt aaggcagtt ggggccagg accggatca ccgtccttt attgaaaga aattagggc tttgaatca caaccggat tattctaga ctgcgacac tgaaaacat catcgaaat tttgaagca agccaaaga agtcaattt gatggaatc gtcatactt taattactt tctaacgtc tgtggacgc acacacttt ggatttttc cgtcttgaa cactactgc caacattac aacaatccg aattttgga tttcacaca aacccaatc aaattactc gactcggac cgaaacggc tctcattca agacggtat tcattcatt tatcacttc tgtgaatac tctccacgt gaccgaacc aattgtacc atcaccaat ggcgtgcga tcaaaaatg gtcacacgg gttcaggat tagttcgtt catgagagt cagagaatt ttttggtgc tagtggccc tattgggtt tgattcatc aggtcgtag gctcaattt cacgatttt tgttgttga aggcaatcg ggaaaatgt tgtagctaa gtggagacc cgaaaatcc agaaatttt gatctacat gcgggggga aaatgatag ttttgatgc gttggttaa aaacatgca aaagagtgt tttgataat ctcttcaag actcggtcc tccatattt aatggaacg caagcctcg tttcgaggg tgaaattct gacagggaa ctcccgtta cgagggttt ttccatgga cctcataga tgccatagc cgggatttt aggttttaa atacgaatt tcgtttggc aaaagacgg caaatatgg caattatga ccccgagac caaatcttg gaacggcct gattaaaga tttattgga tcgaaaagc tgatttggc aatttgcga cctaactat cactcatca aaggcgaga agtggtcga ttttagtat gccatttat gcgactggg gataagtat tttgtacaa aaaagccga agagaaaga tgtcaacat ttttgcttt tttggagcc tttctcgcc cgaaatttg gatttacac ggccacttt gtatctagt tgtttcagt gattttgta tttggtagc aaggatggc ccccggaga ttgggaaaa cccacacgc ttgcaaccc caaacctga aaaacttga aaatatttg gaacctcaa aaattgctt gtggttgac tttggggtc cataatgac ccaaggttg cgacatttt accaaaggg aatttcatc acgactggc aacttcaat gtggtggtt cttttccct aatcatgac aagttcata cacagccaa cttggccgc ttttctcac aatggaacg cctggagcc aaccataga cagcgccga ggctttagc caaacaaac gaaaatcaa atacggaac agttgaagg tggagcaac ccaagcctt ctttcgcga atcgaatta ctcgacgta tcagaaaat gtggactac tatgattca agcaaaacc gggcgtttt tgaaaaaaa taacgcaga tggcgtgaa acgagtcca aacaaccaa gaatcgcct ctacgcctt cctgatgga atcgtcaca gattgagta cgagatcga gacgaaatg cgatttgaa acaagtcgg caattggtt ggataacaa ggagtatgg gattgctat gcccattga ttatccata cagaagtgc cataaatac cgcaatttt gaaactaca agaggaagc aaaattgac cgaactgaa ggacaaatg gtggaaaaa aatgcgcga tgagccctc ttgtcccgt gcgaacact aggaaaaag ttccacaga gttggcgtt ggataatgt tgggggcgt ttttttggt actgggggt aggaatggc ggttgcttt tgttttagc aatcctcga gtttttgtg gaacgttcg aaatatttc agtggagga acacatgac ctattttga agcactcaa ggtcgaatt aatattcgc tttaaatgt ttgggtcac gaaaaagag aaccaaacc gaaaattaa ttcgctaaa ctgcgtttt gcgcaccga gtaataaaa aaccaaact agaaaatcg tgtttatta atcaaaaat attgttttc ataaataat atgaataaa tatgacaag aaatt
