Status
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Gene information
General information
TC012818 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15994988..15998305
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012818
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02938

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012818 RA
      MKILSQNVI WLLIFVAYN YLFCLADDK NKITVGAFF 
      EREDVQSKA ALNYAIDTT NMMQQHLKY ALKTQILAQ 
      NDSFYCCKL LCNVTQEGS GLAAIFASK PIFESLSNR 
      LEIPFILTK WRPASYSNK QTTVNFFPD SYLFSHGLA 
      IIVKNLQWK NFVLLYDSD KGLVKLQQI LKLNNFNSG 
      SVIVRQLGP GPDHRPLLK EIRALNHNR IILDCDTEN 
      IIEILKQAK EVNLMESSY FNYFLTSVD AHTLDFSVL 
      NTTANITTI RILDFTQTQ SNYSTRTET ALIQDGIHS 
      FITSVNTLH VTEPIVPSP MACDQKWSH GFRISSFMR 
      VREFFGASG PIGFDSSGR RLNFTIFVV EGNRENVVA 
      KWRPENPEI LIYMRGEND SFDALVKNM QKSVLIISS 
      RLGPPYLME RKPRFEGEI LTGNSRYEG FSMDLIDAI 
      AGILGFKYE FRLAKDGKY GNYDPETKS WNGLIKDLL 
      DRKADLAIC DLTITHQRR EVVDFSMPF MRLGISILY 
      KKAEEKDVN IFAFLEPFS PEIWIYTAT LYLVVSVIL 
      YLVARMAPG DWENPHACN PKPEKLENI WNLKNCLWL 
      TLGSIMTQG CDILPKGIS SRLATSMWW FFSLIMTSS 
      YTANLAAFL TMERLEPTI DSAEALAKQ TKIKYGTVE 
      GGATQAFFR ESNYSTYQK MWTTMIQAK PGVFEKNNA 
      DGVKRVQTT KNRLYAFLM ESSQIEYEI ETKCDLKQV 
      GNWLDNKEY GIAMPIDYP YRSAINTAI LKLQEEAKL 
      TELKDKWWK KMRDEPSCP VRTLGKSST ELALDNVGG 
      VFLVLGVGM AVAFVLAIL EFLWNVRNI SVEEHMTYF 
      EALKVELIF ALNVWVTKK RTKPKINSL NCVLRTE
    CDS sequence
    • >TC012818 RA
      atgaaaatt ctctcccaa aacgtaatt tggctctta 
      attttcgtc gcttacaat tatctcttc tgtctcgct 
      gatgacaaa aataaaata acagtcggt gcatttttt 
      gaacgtgaa gacgtccaa tctaaagca gccctcaac 
      tatgccata gataccaca aatatgatg caacaacat 
      ttgaaatat gccctcaaa acccaaatt ttagcgcag 
      aatgattcg ttttactgt tgcaaactt ttatgtaat 
      gtgacgcaa gaagggtcg ggattggct gcaattttc 
      gcctcaaaa cctattttc gaatcatta agtaatcga 
      ctagaaatt ccgttcatc ttgacaaaa tggcgcccc 
      gcctcttat tcaaataaa caaacgacg gtcaacttc 
      ttccccgat tcgtatttg ttttcgcac ggtctagcc 
      atcattgtt aaaaatttg caatggaaa aattttgtc 
      ttactttac gacagcgac aaaggtctt gtaaagttg 
      caacaaatt ttaaaattg aataatttc aattccggt 
      tctgtgatt gtaaggcag ttggggcca ggaccggat 
      caccgtcct ttattgaaa gaaattagg gctttgaat 
      cacaaccgg attattcta gactgcgac actgaaaac 
      atcatcgaa attttgaag caagccaaa gaagtcaat 
      ttgatggaa tcgtcatac tttaattac tttctaacg 
      tctgtggac gcacacact ttggatttt tccgtcttg 
      aacactact gccaacatt acaacaatc cgaattttg 
      gatttcaca caaacccaa tcaaattac tcgactcgg 
      accgaaacg gctctcatt caagacggt attcattca 
      tttatcact tctgtgaat actctccac gtgaccgaa 
      ccaattgta ccatcacca atggcgtgc gatcaaaaa 
      tggtcacac gggttcagg attagttcg ttcatgaga 
      gtcagagaa ttttttggt gctagtggc cctattggg 
      tttgattca tcaggtcgt aggctcaat ttcacgatt 
      tttgttgtt gaaggcaat cgggaaaat gttgtagct 
      aagtggaga cccgaaaat ccagaaatt ttgatctac 
      atgcggggg gaaaatgat agttttgat gcgttggtt 
      aaaaacatg caaaagagt gttttgata atctcttca 
      agactcggt cctccatat ttaatggaa cgcaagcct 
      cgtttcgag ggtgaaatt ctgacaggg aactcccgt 
      tacgagggt ttttccatg gacctcata gatgccata 
      gccgggatt ttaggtttt aaatacgaa tttcgtttg 
      gcaaaagac ggcaaatat ggcaattat gaccccgag 
      accaaatct tggaacggc ctgattaaa gatttattg 
      gatcgaaaa gctgatttg gcaatttgc gacctaact 
      atcactcat caaaggcga gaagtggtc gattttagt 
      atgccattt atgcgactg gggataagt attttgtac 
      aaaaaagcc gaagagaaa gatgtcaac atttttgct 
      tttttggag cctttctcg cccgaaatt tggatttac 
      acggccact ttgtatcta gttgtttca gtgattttg 
      tatttggta gcaaggatg gcccccgga gattgggaa 
      aacccacac gcttgcaac cccaaacct gaaaaactt 
      gaaaatatt tggaacctc aaaaattgc ttgtggttg 
      actttgggg tccataatg acccaaggt tgcgacatt 
      ttaccaaag ggaatttca tcacgactg gcaacttca 
      atgtggtgg ttcttttcc ctaatcatg acaagttca 
      tacacagcc aacttggcc gcttttctc acaatggaa 
      cgcctggag ccaaccata gacagcgcc gaggcttta 
      gccaaacaa acgaaaatc aaatacgga acagttgaa 
      ggtggagca acccaagcc ttctttcgc gaatcgaat 
      tactcgacg tatcagaaa atgtggact actatgatt 
      caagcaaaa ccgggcgtt tttgaaaaa aataacgca 
      gatggcgtg aaacgagtc caaacaacc aagaatcgc 
      ctctacgcc ttcctgatg gaatcgtca cagattgag 
      tacgagatc gagacgaaa tgcgatttg aaacaagtc 
      ggcaattgg ttggataac aaggagtat gggattgct 
      atgcccatt gattatcca tacagaagt gccataaat 
      accgcaatt ttgaaacta caagaggaa gcaaaattg 
      accgaactg aaggacaaa tggtggaaa aaaatgcgc 
      gatgagccc tcttgtccc gtgcgaaca ctaggaaaa 
      agttccaca gagttggcg ttggataat gttgggggc 
      gtttttttg gtactgggg gtaggaatg gcggttgct 
      tttgtttta gcaatcctc gagtttttg tggaacgtt 
      cgaaatatt tcagtggag gaacacatg acctatttt 
      gaagcactc aaggtcgaa ttaatattc gctttaaat 
      gtttgggtc acgaaaaag agaaccaaa ccgaaaatt 
      aattcgcta aactgcgtt ttgcgcacc gagtaa
    mRNA sequence
    • >TC012818 RA
      cgtgtcttt gatttatca cacaacaat gaaaattct 
      ctcccaaaa cgtaatttg gctcttaat tttcgtcgc 
      ttacaatta tctcttctg tctcgctga tgacaaaaa 
      taaaataac agtcggtgc attttttga acgtgaaga 
      cgtccaatc taaagcagc cctcaacta tgccataga 
      taccacaaa tatgatgca acaacattt gaaatatgc 
      cctcaaaac ccaaatttt agcgcagaa tgattcgtt 
      ttactgttg caaactttt atgtaatgt gacgcaaga 
      agggtcggg attggctgc aattttcgc ctcaaaacc 
      tattttcga atcattaag taatcgact agaaattcc 
      gttcatctt gacaaaatg gcgccccgc ctcttattc 
      aaataaaca aacgacggt caacttctt ccccgattc 
      gtatttgtt ttcgcacgg tctagccat cattgttaa 
      aaatttgca atggaaaaa ttttgtctt actttacga 
      cagcgacaa aggtcttgt aaagttgca acaaatttt 
      aaaattgaa taatttcaa ttccggttc tgtgattgt 
      aaggcagtt ggggccagg accggatca ccgtccttt 
      attgaaaga aattagggc tttgaatca caaccggat 
      tattctaga ctgcgacac tgaaaacat catcgaaat 
      tttgaagca agccaaaga agtcaattt gatggaatc 
      gtcatactt taattactt tctaacgtc tgtggacgc 
      acacacttt ggatttttc cgtcttgaa cactactgc 
      caacattac aacaatccg aattttgga tttcacaca 
      aacccaatc aaattactc gactcggac cgaaacggc 
      tctcattca agacggtat tcattcatt tatcacttc 
      tgtgaatac tctccacgt gaccgaacc aattgtacc 
      atcaccaat ggcgtgcga tcaaaaatg gtcacacgg 
      gttcaggat tagttcgtt catgagagt cagagaatt 
      ttttggtgc tagtggccc tattgggtt tgattcatc 
      aggtcgtag gctcaattt cacgatttt tgttgttga 
      aggcaatcg ggaaaatgt tgtagctaa gtggagacc 
      cgaaaatcc agaaatttt gatctacat gcgggggga 
      aaatgatag ttttgatgc gttggttaa aaacatgca 
      aaagagtgt tttgataat ctcttcaag actcggtcc 
      tccatattt aatggaacg caagcctcg tttcgaggg 
      tgaaattct gacagggaa ctcccgtta cgagggttt 
      ttccatgga cctcataga tgccatagc cgggatttt 
      aggttttaa atacgaatt tcgtttggc aaaagacgg 
      caaatatgg caattatga ccccgagac caaatcttg 
      gaacggcct gattaaaga tttattgga tcgaaaagc 
      tgatttggc aatttgcga cctaactat cactcatca 
      aaggcgaga agtggtcga ttttagtat gccatttat 
      gcgactggg gataagtat tttgtacaa aaaagccga 
      agagaaaga tgtcaacat ttttgcttt tttggagcc 
      tttctcgcc cgaaatttg gatttacac ggccacttt 
      gtatctagt tgtttcagt gattttgta tttggtagc 
      aaggatggc ccccggaga ttgggaaaa cccacacgc 
      ttgcaaccc caaacctga aaaacttga aaatatttg 
      gaacctcaa aaattgctt gtggttgac tttggggtc 
      cataatgac ccaaggttg cgacatttt accaaaggg 
      aatttcatc acgactggc aacttcaat gtggtggtt 
      cttttccct aatcatgac aagttcata cacagccaa 
      cttggccgc ttttctcac aatggaacg cctggagcc 
      aaccataga cagcgccga ggctttagc caaacaaac 
      gaaaatcaa atacggaac agttgaagg tggagcaac 
      ccaagcctt ctttcgcga atcgaatta ctcgacgta 
      tcagaaaat gtggactac tatgattca agcaaaacc 
      gggcgtttt tgaaaaaaa taacgcaga tggcgtgaa 
      acgagtcca aacaaccaa gaatcgcct ctacgcctt 
      cctgatgga atcgtcaca gattgagta cgagatcga 
      gacgaaatg cgatttgaa acaagtcgg caattggtt 
      ggataacaa ggagtatgg gattgctat gcccattga 
      ttatccata cagaagtgc cataaatac cgcaatttt 
      gaaactaca agaggaagc aaaattgac cgaactgaa 
      ggacaaatg gtggaaaaa aatgcgcga tgagccctc 
      ttgtcccgt gcgaacact aggaaaaag ttccacaga 
      gttggcgtt ggataatgt tgggggcgt ttttttggt 
      actgggggt aggaatggc ggttgcttt tgttttagc 
      aatcctcga gtttttgtg gaacgttcg aaatatttc 
      agtggagga acacatgac ctattttga agcactcaa 
      ggtcgaatt aatattcgc tttaaatgt ttgggtcac 
      gaaaaagag aaccaaacc gaaaattaa ttcgctaaa 
      ctgcgtttt gcgcaccga gtaataaaa aaccaaact 
      agaaaatcg tgtttatta atcaaaaat attgttttc 
      ataaataat atgaataaa tatgacaag aaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY