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Gene information
General information
TC012811 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 16085691..16086595
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012811
    iBeetle screen iB_02937

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature partially ectopicallypresent - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    head sometimes partiallyremaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    posterior terminus sometimes remaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012811 RA
      MQLTLVSVL CVIKVTAGG WLRDLISPK 
      GWDIGDGVV VHLVDSKLP NTTFLQRHS 
      MVMDYGGLA LSVSQALDK EALRVGLHP 
      SGRGYKDYT ESAAVSMII GYKLATMAT 
      LAFSAVGAL VLKALSVAK IAAVLSMIL 
      LVSKLFHHN QHQQQQHQL PAYIEIEPE 
      FAEISPEEY FPSASSDYY SYASFPPGI 
      DYVPETSGG NSTPPGDQQ VSRRRDVSK 
      RRRVPLLTI HQYSIKS
    CDS sequence
    • >TC012811 RA
      atgcagctt actcttgtt agtgtcttg tgtgtcatc 
      aaagtcaca gccggtggc tggttgaga gatttaatt 
      tctcctaaa ggatgggac attggggat ggagttgtt 
      gttcattta gtcgattca aaactcccc aacaccact 
      tttttgcaa agacattct atggttatg gattacggc 
      ggattggct cttagtgtg agtcaagct cttgataaa 
      gaagcttta cgagttggt ctccaccct agcggacga 
      ggttataag gattacaca gaatccgct gctgttagt 
      atgatcatc ggttataag ctcgcaacg atggcaact 
      cttgccttt tccgctgtg ggagcttta gttttgaaa 
      gctctcagc gttgctaaa attgcagcc gtgctttcg 
      atgatcctt ttagtatcg aagcttttc catcacaat 
      caacaccaa cagcaacaa catcaactt ccggcttat 
      attgaaatt gaaccagaa tttgctgaa atttctcct 
      gaagagtat tttccgtca gcatcgagt gattattat 
      tcgtacgcg agctttcct ccggggatt gattacgta 
      ccagagaca tctggtggc aattcgacg cctccgggc 
      gatcaacaa gtgtcgaga cgtcgagac gtttcaaaa 
      cgaagaaga gtgccgttg ttgacgata catcaatat 
      tccattaag tcttga
    mRNA sequence
    • >TC012811 RA
      tctttaata taaatgtca gagcaaagt cttctcttt 
      gccataatg cagcttact cttgttagt gtcttgtgt 
      gtcatcaaa gtcacagcc ggtggctgg ttgagagat 
      ttaatttct cctaaagga tgggacatt ggggatgga 
      gttgttgtt catttagtc gattcaaaa ctccccaac 
      accactttt ttgcaaaga cattctatg gttatggat 
      tacggcgga ttggctctt agtgtgagt caagctctt 
      gataaagaa gctttacga gttggtctc caccctagc 
      ggacgaggt tataaggat tacacagaa tccgctgct 
      gttagtatg atcatcggt tataagctc gcaacgatg 
      gcaactctt gccttttcc gctgtggga gctttagtt 
      ttgaaagct ctcagcgtt gctaaaatt gcagccgtg 
      ctttcgatg atcctttta gtatcgaag cttttccat 
      cacaatcaa caccaacag caacaacat caacttccg 
      gcttatatt gaaattgaa ccagaattt gctgaaatt 
      tctcctgaa gagtatttt ccgtcagca tcgagtgat 
      tattattcg tacgcgagc tttcctccg gggattgat 
      tacgtacca gagacatct ggtggcaat tcgacgcct 
      ccgggcgat caacaagtg tcgagacgt cgagacgtt 
      tcaaaacga agaagagtg ccgttgttg acgatacat 
      caatattcc attaagtct tgaagcgat gacattttt 
      ctcttattt aagtgtatt aggtattat tgaataaag 
      cgttatgag attt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY