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Gene information
General information
TC012751 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid67: 6409637..6411597
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012751
    iBeetle screen iB_02935
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012751 RA
      MHSIAASAN MTSEIIPRP CYDSIANKL KTLLSFAVG 
      GLLGDVFLH SLPEIWAND AARNGGQPT TTTGLLILT 
      GLIVFVVTE KLFTVIEKL GERHLAPPE NNNVKGYKH 
      IPEPKKHIT GYLNLLANT IDNFTHGLS LGGAFLVSL 
      RLGLLTTFA ILVHEIPHE VGDFAILLK SGFTRWDAA 
      FYQILTAGG GLVGSFAAI MCSGASNSI EARTSWIMP 
      FTAGTFLHI ALVTVLPDL LKEEDPRES LKQILALIA 
      GISVMAIVT TLVE
    CDS sequence
    • >TC012751 RA
      atgcattca attgctgct tccgcgaat atgacttca 
      gagattatt ccaaggccg tgttacgac tccattgcg 
      aacaaattg aagacgttg ctgagcttc gccgtcgga 
      ggcctcctt ggcgacgtc ttcctgcac tcgcttccg 
      gaaatatgg gccaatgat gcggcccga aacggaggt 
      cagccgact acaacgacc ggtcttctc atcctcacc 
      ggtttgata gtatttgta gtgacagag aagctattc 
      actgtgata gaaaaactg ggagagcgc catttggcg 
      cccccggaa aacaacaac gtcaaagga tacaaacat 
      atccccgag cccaagaag cacataacc ggctatttg 
      aaccttttg gccaacaca atcgacaac ttcacccac 
      ggcttatcc ctcggtggg gctttcctg gtatcgctc 
      cgtctgggt ctcttgacc acctttgcc atcctcgtc 
      cacgaaatc ccgcacgag gttggcgat tttgccatt 
      ttgctcaaa tcgggcttc acacgatgg gacgccgcc 
      ttttaccag attttaaca gctgggggc ggcctggtc 
      ggctcgttc gccgccatc atgtgcagc ggagcctcc 
      aattcaata gaggcccgc acgtcgtgg attatgccc 
      ttcacggcc gggacgttc ctccacatc gcgctggtc 
      acggtcctc ccggattta ctcaaagag gaggacccc 
      cgggagtca ctcaagcag atcctcgcc cttattgca 
      gggatcagt gtcatggcc atcgtgacg actttagtt 
      gagtga
    mRNA sequence
    • >TC012751 RA
      tgggcattg tacgaaatg acgcaattt gaggatgat 
      tgacattta cccacttga tttggcacc cttgacagc 
      cgtagaaat aaattttat gaaaattgc atgcatatg 
      gtcgattag ataaagccg gcacgtcca tgataattc 
      attgatgat tcgaattac aaaacaatt cagccgttg 
      gaaaaaatg cattcaatt gctgcttcc gcgaatatg 
      acttcagag attattcca aggccgtgt tacgactcc 
      attgcgaac aaattgaag acgttgctg agcttcgcc 
      gtcggaggc ctccttggc gacgtcttc ctgcactcg 
      cttccggaa atatgggcc aatgatgcg gcccgaaac 
      ggaggtcag ccgactaca acgaccggt cttctcatc 
      ctcaccggt ttgatagta tttgtagtg acagagaag 
      ctattcact gtgatagaa aaactggga gagcgccat 
      ttggcgccc ccggaaaac aacaacgtc aaaggatac 
      aaacatatc cccgagccc aagaagcac ataaccggc 
      tatttgaac cttttggcc aacacaatc gacaacttc 
      acccacggc ttatccctc ggtggggct ttcctggta 
      tcgctccgt ctgggtctc ttgaccacc tttgccatc 
      ctcgtccac gaaatcccg cacgaggtt ggcgatttt 
      gccattttg ctcaaatcg ggcttcaca cgatgggac 
      gccgccttt taccagatt ttaacagct gggggcggc 
      ctggtcggc tcgttcgcc gccatcatg tgcagcgga 
      gcctccaat tcaatagag gcccgcacg tcgtggatt 
      atgcccttc acggccggg acgttcctc cacatcgcg 
      ctggtcacg gtcctcccg gatttactc aaagaggag 
      gacccccgg gagtcactc aagcagatc ctcgccctt 
      attgcaggg atcagtgtc atggccatc gtgacgact 
      ttagttgag tgataagtg ataagaatt cagttgcca 
      tattgttga tagttttgt tgtaaatta cagtattag 
      ttgtaattt ttgttttct ttaggtagg gcattaagc 
      gaaaaccat atttttcca gttgttagc atcgtctga 
      tggtaatca gactgaact atttttgtg atattaaca 
      ttttttcaa cacaatata ttttattat tctgtagta 
      cttaaagtg ctcttatat attttatat caaaataaa 
      acatgacta cgatgatat attgtaaat aaagagcaa 
      gaaacgac