Status
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Gene information
General information
TC012712 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13551419..13576477
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012712
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02933

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012712 RA
      MKRGKPQQE IYRPGSGPL RKSNTGNEE SDPDLNLKP 
      KPYTHDDKY KPETITEPH RKPRKPEQM IYVPRAVAN 
      AREMSQDND RHALNGNFD GFTPRSKRY SSRRRGGGE 
      NDHDEWRQM RQGSEPRGV SNGAHSRMR DTRSVEPTG 
      PPPRNYEKA HPKPPSGRR HSTIGVESD KRPPHKPIN 
      VDKLPPRLR KKMLEDNKL NQAVEDDWN GASLTFQGS 
      SNYHPVGGY YPNTNQNYY QNMGYCTLP NRHRGRGRL 
      QQEGEYRSR TPDLGISSP CNSRPPTPP YGRTRSNDN 
      LNRVDRPAS PRNYDYRRN GRERNNRRN YARNRDDRR 
      NQNYEVQEE NWDEEERVS QKVETREST PPVTPPTPE 
      EKIKITVPS SSNTILDWS EEVELHDQL EAEALSDAM 
      TRSSSVASL VDISVKSMP PNINNQKKS KRRSNRRKG 
      RSGSRPRDS SLDNPKSAP RDTTFRAPQ EPRGRRNRR 
      HSRDRRDSS YDRRSRNPS RDTSFDRNR RSSTTEPEN 
      WREEIRSRQ NSEREFSDR SRRNSERET EKINVKKAG 
      VLILPPKQQ DVAVTHVDQ PRYPEVRKP CQQKSLFDH 
      NNPSKPIIV KSQSSRVSV PGFSDNTET TPPQMYTTD 
      QFGNIRPGW YDENSESFN SCHYPNLIR DIKRADNEL 
      QYIMNSGQI LINWGTVES LRQFLKEAL QYVLCKDLK 
      FCEAENVEQ HLWKILYHN IIEVTRKAI TNDPGNKEQ 
      YKGFLLYLI DEGTSYFEG LLDSLEETY KFKLSNFLG 
      NNNNAQKGL GYVGLALIS AQKLLLFLG DLGRYREQV 
      NETSNYGKC RQWYIKAHE INPKNGKPY NQLAVLAVY 
      ARRKLDAVY YYMRSLMSS NPVPSAREY LISLFDENR 
      KKYEQGERK RREERLERT KQHMKQKES EDPENTPGS 
      LRHETWIRP DGGRRVHRT TQAVQEQQD SEEEDLAAL 
      SSVEVNKRF VISYLHVHG KLITRIGME SFQEAAMQM 
      LKEFRALLQ HSPVPLPCN RFLQLLALN MFAIESTQL 
      KDPQLQMQA GYRSELQER ALVVSLQMF NLILERCVS 
      LLQESNPEN LNSLHQDVH VLLPAVKIW CDWMLCHSA 
      VWNPPPSTQ DFRVGSSGD AWSRLATLM NILEKLDQT 
      SVNALVSDY QEGYEQVKL PEDIVLNGF TPLMVNEPD 
      ISYAPKDLD VEVAQFALR MNKLLFFGT VFLCGLEPP 
      VLKLEIEDD FREYVSVVC TSNSRESPV TAQDLVRNN 
      DVLMESFSD EEEEEILSG PGEEAPSEV RELLHRKVE 
      LERTHRRQE LHKQRVKKI LSQAVVSVH IEVRPKYLV 
      PDTNCFIDH LDPITTITS SHVYTLMVP IVVLSELEG 
      LSRGGKSPI PSTRSMLDP QHVRKVAQA AKRALNFLK 
      SRPQAVKCV TTKGAVLSS TTFTTEDDS MLDATFKND 
      DKILATCLV LSKGHREQQ QIAENEPKK LVSEVVLLT 
      DDRNLRVKA LARDVPVRE LLDFMQWAG LG
    CDS sequence
    • >TC012712 RA
      atgaaacgt ggaaaacca caacaggag atttacaga 
      ccaggcagt ggaccttta agaaaatca aacacagga 
      aacgaagag tctgacccc gacctaaac ctcaaacct 
      aagccttac acacacgat gacaagtac aaaccggaa 
      acaataacc gaaccgcac cgcaagccg cgcaaaccg 
      gagcaaatg atttacgtc ccgagggcg gtggcaaac 
      gcccgcgag atgtctcaa gacaacgac agacacgcc 
      cttaacggc aacttcgac gggttcacc ccccggtcc 
      aagcggtac tccagccgg cggcgaggg ggcggcgaa 
      aacgaccac gacgagtgg cggcagatg cggcagggc 
      tcggagccg cggggcgtc tccaacggg gcccactcg 
      cgcatgcgg gacactcgg agcgtggag ccgacgggg 
      ccgccgccc cgcaactac gaaaaagcc cacccgaag 
      ccccccagc ggccgccga cacagcacg atcggcgtg 
      gaatcggat aaaaggccc cctcataaa ccgattaac 
      gtcgataaa ctaccccca agactgcgc aaaaaaatg 
      ctagaagac aataaactg aaccaagcg gtggaagat 
      gactggaac ggcgcgagt ttgacgttt caaggcagt 
      agtaattat cacccagtt ggggggtac taccccaac 
      acaaaccaa aactactat caaaatatg gggtattgt 
      actttgccg aaccgccac aggggtcgt gggcgtttg 
      cagcaagag ggcgagtac aggtcgaga accccagat 
      ttggggatt tcgagtcca tgtaattcg aggcctccg 
      acgcccccc tatggtagg actagaagc aacgataat 
      ttgaatagg gtggatagg ccggcatcg ccgaggaat 
      tacgattat aggcgcaat gggcgcgag aggaataat 
      aggaggaat tatgcgagg aatagggat gataggaga 
      aatcaaaat tatgaagtg caagaagaa aactgggat 
      gaggaggag agagtgagt cagaaggtt gaaacacgg 
      gaaagtaca ccaccggta acgccccca acgccagaa 
      gaaaaaatc aaaatcaca gttcctagc agcagcaat 
      acgattttg gattggagc gaagaagtc gaactacac 
      gaccagctt gaagctgaa gctttaagc gacgcaatg 
      acacgaagt tcatccgta gctagttta gtcgacatt 
      agtgtcaaa tcaatgcct ccgaatatt aacaatcaa 
      aaaaaatca aaaagacgc tctaaccgc cgcaagggg 
      cgcagcggc agccgcccc cgcgactcc agcctcgac 
      aacccaaaa tccgcccct cgcgacacc accttccgc 
      gccccccag gagccccga ggccgtcga aaccggcgc 
      cattcgcgc gaccgtcgc gattccagc tacgaccga 
      cgttcgcgc aaccccagc cgagacacc agcttcgac 
      cgcaaccga cgcagttcc accaccgag ccggaaaac 
      tggcgcgag gaaattcgc agtcggcaa aactccgaa 
      cgcgagttt tccgaccga agcagacgc aactcggaa 
      cgcgaaacg gagaagatc aacgtgaaa aaagccggg 
      gtcttgatc cttccaccg aaacaacaa gacgttgct 
      gtgactcac gtcgaccaa ccgcggtac ccggaagtc 
      cggaaaccc tgtcaacag aagagtctc ttcgatcat 
      aataatccc agcaaaccg atcattgtt aaatcgcaa 
      agttcgagg gtcagtgtg cccggattt tccgacaat 
      acggaaacg acgcccccc cagatgtac accactgat 
      cagttcggg aacatcaga ccggggtgg tacgacgaa 
      aactcggaa agttttaac tcgtgccat tatcctaac 
      ttaatcaga gacataaag cgagccgat aatgaacta 
      cagtacatc atgaattcg ggtcagatt ttgataaat 
      tggggcacg gtcgagtcg ctgagacag ttcctgaaa 
      gaagccctc cagtacgta ttgtgcaag gacttgaag 
      ttttgtgag gcggaaaat gtcgagcaa cacttgtgg 
      aaaatcctc taccacaat attatcgaa gtgacccgg 
      aaggcgata acaaacgat cccgggaat aaggaacag 
      tacaaggga tttttgctg tatttgatc gacgaaggc 
      acgagttat ttcgagggt ttgctggac tcgttagaa 
      gagacttac aaattcaaa ctgagcaat tttttgggt 
      aacaataac aatgcgcag aaaggtttg ggttacgtg 
      ggacttgcc ttaatttcc gcccagaag ttgctcttg 
      tttttgggc gatttgggc cgatatcgc gaacaagtc 
      aacgaaacc tcgaattat ggaaagtgc cgccagtgg 
      tacatcaaa gcgcacgaa attaatccg aaaaatggc 
      aagccctat aaccaactg gcggtactt gcggtgtat 
      gctaggcga aaattggac gctgtttat tactacatg 
      aggagcttg atgtcgtca aatccggtc ccttccgct 
      cgggaatat ttaatttcg ttgtttgac gaaaacaga 
      aaaaagtac gaacagggc gaacgcaag cggcgcgaa 
      gagcgtctc gagcgcacc aaacaacac atgaaacag 
      aaagagtcc gaagatccg gaaaacacg cccggatcg 
      ctccggcac gagacctgg attcgtccg gacggcggg 
      cgtcgagtc catcggacg acgcaagcc gttcaagaa 
      caacaagac tcagaagag gaggacttg gcggcgctg 
      tcgagcgtc gaagtcaac aagagattc gttatcagc 
      tatctgcat gtccacgga aagctaatc acgcgcatt 
      ggaatggag agtttccag gaggccgcc atgcagatg 
      ctaaaggag tttcgggcg ttgttgcaa cactctccc 
      gtgcctttg ccctgcaac cgattcctg cagttgctg 
      gcgcttaac atgttcgcc atcgaaagc acccaattg 
      aaagatcct cagctgcag atgcaggcg ggttatcgc 
      tccgagtta caggagcgt gcccttgtc gtatctctg 
      caaatgttc aatttgata ctagagcgt tgcgtttcg 
      ctcctgcag gagagcaac ccagaaaat ctcaattct 
      ctgcaccag gacgttcac gttctctta ccggccgtg 
      aaaatttgg tgcgattgg atgctttgt cactcggcg 
      gtttggaac ccaccaccg tccactcag gatttccga 
      gtcgggagt tcgggtgat gcatggtcg cgtctcgcg 
      acactgatg aacatcttg gagaagtta gatcagaca 
      tcagtcaac gccctcgtc tcggattat caggaaggt 
      tacgaacaa gtcaagctg ccagaggac attgtcttg 
      aatggtttt acgccgctg atggtgaac gagcccgat 
      atcagctac gcgcccaaa gatttggac gtcgaagtg 
      gcacagttc gccttgagg atgaacaaa ttgctattt 
      ttcggaacg gtttttttg tgcggcttg gaaccgccc 
      gttttgaaa ttggaaatt gaagacgat tttagggag 
      tacgtctcg gtggtttgc acgtcgaat agtagagaa 
      tcaccggtg acggcgcag gatttggtt cgcaacaac 
      gacgtcctg atggagagt ttcagcgac gaagaggag 
      gaggagatt ttgagcggt cctggcgag gaggcaccg 
      tctgaagtt cgggaattg ttacaccgc aaggtggaa 
      ctcgagagg acccacagg cgacaagaa ttacacaaa 
      caacgtgta aagaaaatc ttgagccag gccgtggtc 
      agcgtgcac atcgaggtg cgtcccaag tacctcgtg 
      cccgacacc aactgcttc atcgaccac ctggacccc 
      atcaccacc ataacgtct tcacacgtc tacaccctc 
      atggtgccc atcgtcgta ttgagcgaa ctggagggt 
      ttaagtagg ggcggaaaa tcgccaata ccgtcaaca 
      cgcagcatg ctagatccg caacatgtg agaaaagtt 
      gcacaagcc gcgaaaaga gcgttgaat tttctcaag 
      agtcgaccg caagctgtg aaatgcgtt acgacgaaa 
      ggagcggtt ttgtcgtcg acgactttt acgactgaa 
      gatgattcc atgttggac gcaacgttc aaaaacgac 
      gataagatt ttagccacg tgtctggtt ctaagcaag 
      ggacacagg gagcagcaa caaattgcg gaaaacgaa 
      cctaagaaa ttagtaagc gaagtcgta cttttgacg 
      gacgaccga aacttgcgc gtgaaagca ttagcaaga 
      gacgtgccc gtgagggag ctcttagat ttcatgcag 
      tgggctgga ttaggatga
    mRNA sequence
    • >TC012712 RA
      atttttatc ggaaactaa ttatttgaa cgaaattca 
      tccgttgcg tttgccaat gcgaaaggt tggaaaaag 
      tactagaag ctgaaaaga aaaatatga aacgtggaa 
      aaccacaac aggagattt acagaccag gcagtggac 
      ctttaagaa aatcaaaca caggaaacg aagagtctg 
      accccgacc taaacctca aacctaagc cttacacac 
      acgatgaca agtacaaac cggaaacaa taaccgaac 
      cgcaccgca agccgcgca aaccggagc aaatgattt 
      acgtcccga gggcggtgg caaacgccc gcgagatgt 
      ctcaagaca acgacagac acgccctta acggcaact 
      tcgacgggt tcacccccc ggtccaagc ggtactcca 
      gccggcggc gagggggcg gcgaaaacg accacgacg 
      agtggcggc agatgcggc agggctcgg agccgcggg 
      gcgtctcca acggggccc actcgcgca tgcgggaca 
      ctcggagcg tggagccga cggggccgc cgccccgca 
      actacgaaa aagcccacc cgaagcccc ccagcggcc 
      gccgacaca gcacgatcg gcgtggaat cggataaaa 
      ggccccctc ataaaccga ttaacgtcg ataaactac 
      ccccaagac tgcgcaaaa aaatgctag aagacaata 
      aactgaacc aagcggtgg aagatgact ggaacggcg 
      cgagtttga cgtttcaag gcagtagta attatcacc 
      cagttgggg ggtactacc ccaacacaa accaaaact 
      actatcaaa atatggggt attgtactt tgccgaacc 
      gccacaggg gtcgtgggc gtttgcagc aagagggcg 
      agtacaggt cgagaaccc cagatttgg ggatttcga 
      gtccatgta attcgaggc ctccgacgc ccccctatg 
      gtaggacta gaagcaacg ataatttga atagggtgg 
      ataggccgg catcgccga ggaattacg attataggc 
      gcaatgggc gcgagagga ataatagga ggaattatg 
      cgaggaata gggatgata ggagaaatc aaaattatg 
      aagtgcaag aagaaaact gggatgagg aggagagag 
      tgagtcaga aggttgaaa cacgggaaa gtacaccac 
      cggtaacgc ccccaacgc cagaagaaa aaatcaaaa 
      tcacagttc ctagcagca gcaatacga ttttggatt 
      ggagcgaag aagtcgaac tacacgacc agcttgaag 
      ctgaagctt taagcgacg caatgacac gaagttcat 
      ccgtagcta gtttagtcg acattagtg tcaaatcaa 
      tgcctccga atattaaca atcaaaaaa aatcaaaaa 
      gacgctcta accgccgca aggggcgca gcggcagcc 
      gcccccgcg actccagcc tcgacaacc caaaatccg 
      cccctcgcg acaccacct tccgcgccc cccaggagc 
      cccgaggcc gtcgaaacc ggcgccatt cgcgcgacc 
      gtcgcgatt ccagctacg accgacgtt cgcgcaacc 
      ccagccgag acaccagct tcgaccgca accgacgca 
      gttccacca ccgagccgg aaaactggc gcgaggaaa 
      ttcgcagtc ggcaaaact ccgaacgcg agttttccg 
      accgaagca gacgcaact cggaacgcg aaacggaga 
      agatcaacg tgaaaaaag ccggggtct tgatccttc 
      caccgaaac aacaagacg ttgctgtga ctcacgtcg 
      accaaccgc ggtacccgg aagtccgga aaccctgtc 
      aacagaaga gtctcttcg atcataata atcccagca 
      aaccgatca ttgttaaat cgcaaagtt cgagggtca 
      gtgtgcccg gattttccg acaatacgg aaacgacgc 
      ccccccaga tgtacacca ctgatcagt tcgggaaca 
      tcagaccgg ggtggtacg acgaaaact cggaaagtt 
      ttaactcgt gccattatc ctaacttaa tcagagaca 
      taaagcgag ccgataatg aactacagt acatcatga 
      attcgggtc agattttga taaattggg gcacggtcg 
      agtcgctga gacagttcc tgaaagaag ccctccagt 
      acgtattgt gcaaggact tgaagtttt gtgaggcgg 
      aaaatgtcg agcaacact tgtggaaaa tcctctacc 
      acaatatta tcgaagtga cccggaagg cgataacaa 
      acgatcccg ggaataagg aacagtaca agggatttt 
      tgctgtatt tgatcgacg aaggcacga gttatttcg 
      agggtttgc tggactcgt tagaagaga cttacaaat 
      tcaaactga gcaattttt tgggtaaca ataacaatg 
      cgcagaaag gtttgggtt acgtgggac ttgccttaa 
      tttccgccc agaagttgc tcttgtttt tgggcgatt 
      tgggccgat atcgcgaac aagtcaacg aaacctcga 
      attatggaa agtgccgcc agtggtaca tcaaagcgc 
      acgaaatta atccgaaaa atggcaagc cctataacc 
      aactggcgg tacttgcgg tgtatgcta ggcgaaaat 
      tggacgctg tttattact acatgagga gcttgatgt 
      cgtcaaatc cggtccctt ccgctcggg aatatttaa 
      tttcgttgt ttgacgaaa acagaaaaa agtacgaac 
      agggcgaac gcaagcggc gcgaagagc gtctcgagc 
      gcaccaaac aacacatga aacagaaag agtccgaag 
      atccggaaa acacgcccg gatcgctcc ggcacgaga 
      cctggattc gtccggacg gcgggcgtc gagtccatc 
      ggacgacgc aagccgttc aagaacaac aagactcag 
      aagaggagg acttggcgg cgctgtcga gcgtcgaag 
      tcaacaaga gattcgtta tcagctatc tgcatgtcc 
      acggaaagc taatcacgc gcattggaa tggagagtt 
      tccaggagg ccgccatgc agatgctaa aggagtttc 
      gggcgttgt tgcaacact ctcccgtgc ctttgccct 
      gcaaccgat tcctgcagt tgctggcgc ttaacatgt 
      tcgccatcg aaagcaccc aattgaaag atcctcagc 
      tgcagatgc aggcgggtt atcgctccg agttacagg 
      agcgtgccc ttgtcgtat ctctgcaaa tgttcaatt 
      tgatactag agcgttgcg tttcgctcc tgcaggaga 
      gcaacccag aaaatctca attctctgc accaggacg 
      ttcacgttc tcttaccgg ccgtgaaaa tttggtgcg 
      attggatgc tttgtcact cggcggttt ggaacccac 
      caccgtcca ctcaggatt tccgagtcg ggagttcgg 
      gtgatgcat ggtcgcgtc tcgcgacac tgatgaaca 
      tcttggaga agttagatc agacatcag tcaacgccc 
      tcgtctcgg attatcagg aaggttacg aacaagtca 
      agctgccag aggacattg tcttgaatg gttttacgc 
      cgctgatgg tgaacgagc ccgatatca gctacgcgc 
      ccaaagatt tggacgtcg aagtggcac agttcgcct 
      tgaggatga acaaattgc tatttttcg gaacggttt 
      ttttgtgcg gcttggaac cgcccgttt tgaaattgg 
      aaattgaag acgatttta gggagtacg tctcggtgg 
      tttgcacgt cgaatagta gagaatcac cggtgacgg 
      cgcaggatt tggttcgca acaacgacg tcctgatgg 
      agagtttca gcgacgaag aggaggagg agattttga 
      gcggtcctg gcgaggagg caccgtctg aagttcggg 
      aattgttac accgcaagg tggaactcg agaggaccc 
      acaggcgac aagaattac acaaacaac gtgtaaaga 
      aaatcttga gccaggccg tggtcagcg tgcacatcg 
      aggtgcgtc ccaagtacc tcgtgcccg acaccaact 
      gcttcatcg accacctgg accccatca ccaccataa 
      cgtcttcac acgtctaca ccctcatgg tgcccatcg 
      tcgtattga gcgaactgg agggtttaa gtaggggcg 
      gaaaatcgc caataccgt caacacgca gcatgctag 
      atccgcaac atgtgagaa aagttgcac aagccgcga 
      aaagagcgt tgaattttc tcaagagtc gaccgcaag 
      ctgtgaaat gcgttacga cgaaaggag cggttttgt 
      cgtcgacga cttttacga ctgaagatg attccatgt 
      tggacgcaa cgttcaaaa acgacgata agattttag 
      ccacgtgtc tggttctaa gcaagggac acagggagc 
      agcaacaaa ttgcggaaa acgaaccta agaaattag 
      taagcgaag tcgtacttt tgacggacg accgaaact 
      tgcgcgtga aagcattag caagagacg tgcccgtga 
      gggagctct tagatttca tgcagtggg ctggattag 
      gatgaacct cgaagaatc tcaggcgct aatatgagc 
      agtttctag aaacaaggg cgagatctc gagataacg 
      gtaagtaac agcgattac cgacgagtt agctgcaac 
      gtctcgtgt gatttttcc agatttatg acctacgta 
      cttctcacc tagacaaga gttttctgt acttgttta 
      agttaaatt aatcgttgt agtttattt aatttagcg 
      tggggtttg atgtcaccc tcttaactg tttcacacg 
      tttttcaat cgggttgtg agatgttgc attcctcgt 
      gtctcccga ttagatcac gcatacacc gtcacaaaa 
      ttagcgcat cgtgccatt gttttttct aattatatt 
      tatgtaatt gatttttta tatacatat ttatccctc 
      tcagacgtt gagttttat ttttctcta gcacttagt 
      gagatattg tatgtactt attttaatt ttttataat 
      cgtgttatg cacactcta aaccgattg ctttcccgg 
      tgtattcaa aaggaaggc gaagactcc gggaacgca 
      attgacttg gagcgtaac attttgtct taatcccta 
      atgaattaa ttaattaat taaaagatt ttaacttaa 
      acgctgtat ttaggattt atacaatta tacgaactg 
      ttgttaatt attgcaatt gttttgctc aggaattta 
      ttgtacata aataaataa tatgattac gt
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