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Gene information
General information
TC011794 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13457672..13463324
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011794
    iBeetle screen iB_02932

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011794 RA
      MSQKIVLTG TLDMLHSLY DFKATYAKT LSFRTNEYF 
ILHQSNTKH KNWWEVINE AGEMGFIPS NYVETVTVN 
PSFYLQFVD NCLENLKKR APVENLTDR HEIILRLKE 
VKKEIEELP EVGGHGDDL KTLHYKNSD MGRFESMSS 
QSSLLTELK NDIEEPIKP VKANKERVR KSIENIHEE 
VISECCNDV KKAELPSPA ITDQSVYEL VESVRINTQ 
LSYEMSRVA VVTVVQGLH ELLPASVFP YLSTILSQV 
QKSLNVDDV QIDQTHDAS RLKIIFNEL TSCKEDSQQ 
RSWMLHEDE DVIKEYLLE LISILSNAD ASISRHVIS 
RDQYHCIVT LIQFYQMEI RWSIRQLLL QTFGVLCSL 
DKTVVSIML NSILPGELA RDMMANARN IPKLNYSSI 
LLTMIFSMG EPMPITHHE LLGANFLSF LLNNIEYPP 
DTDIDEQIP DLFLNCVIS FNLQFEDCD ENPVLSALE 
ERDVAKTFT EKILLLLNR EHDPVRIFE HEPAPPHAL 
LKLFVDLLS RKTTAALFY TNDIKVLID IVVRNVADL 
SPGDKRRQQ YIELCRLVM RNTNYDEHR HRLDDILKS 
FTRIFCEES ELSKTDQQL VREISHEFP HFFK
    CDS sequence
    • >TC011794 RA
      atgtcacag aagatagtt ttaacaggc accctcgac 
atgctccac agtctctac gacttcaaa gcaacgtac 
gcaaaaacc ctcagtttt cgcaccaac gaatacttc 
atcctgcac caaagcaac accaagcac aaaaactgg 
tgggaagtg ataaacgaa gctggggaa atggggttc 
atcccctcg aactacgtc gagactgtc actgtgaac 
ccctcgttt tacctccag ttcgtcgac aactgtctc 
gagaacctg aagaagcgc gcgccggtg gagaacctc 
accgaccgc cacgaaatc atcctgcgc ttgaaagaa 
gtgaagaag gagatcgag gagctgccc gaggtcggg 
ggtcacggg gacgacctc aagacgtta cattacaaa 
aactcggat atggggcgg tttgagagc atgagctcg 
cagtcttcg ctcctaacc gaactaaaa aacgacatt 
gaggagccc atcaagcct gtgaaagcg aacaaagaa 
cgcgtgagg aagtccatc gaaaacatt cacgaggag 
gtgatttcc gagtgttgc aacgacgtg aaaaaggca 
gagctgccc tcgcccgca atcacagac cagtccgtc 
tacgagctg gttgagagt gtgaggatt aatacgcag 
ttgtcttac gaaatgtcg cgggtggct gtggttacg 
gtcgttcag gggctgcac gagctgctg ccagcgtcg 
gtgtttcca tacttaagc acgattctg tcacaagta 
caaaaatcg ctcaatgtg gatgacgtc caaattgac 
caaacgcac gatgctagt cgacttaag atcatcttt 
aatgagctc acttcgtgt aaggaggac tcgcagcag 
aggagttgg atgcttcat gaagatgag gatgtgatc 
aaagagtat ctcttggag ttaatctcg attttgtca 
aacgccgat gcgagcatt tctcgccat gtcatttcc 
cgagaccaa taccactgt atcgtcacc ctgatccag 
ttttaccaa atggaaatc cgctggtcg atccgccag 
ctcttactc caaacgttc ggcgttctg tgcagtctg 
gacaaaacc gtcgtcagc ataatgctc aactcgatc 
ctcccagga gagcttgcc agggatatg atggcgaac 
gctcgcaac attcccaaa ttaaactac tcctccatt 
ctcctcacc atgattttc tccatgggc gagcccatg 
cccatcacc caccacgaa ctactcggg gcgaatttc 
ttgtcattc ctcctcaat aacattgag tatccgccc 
gatacggac atcgacgag caaatcccc gatttgttc 
ctcaattgc gtcatttcg ttcaatttg cagtttgag 
gactgtgac gaaaatccg gtcctcagc gcgttagag 
gagcgcgac gttgcgaaa accttcacg gagaaaatc 
ctcctcctc ctcaaccgc gagcacgat ccggttcgc 
atttttgag cacgaaccg gcgccgcct cacgccctc 
ctcaagctt tttgtggac ttgctgagt cgcaaaacc 
actgctgcg cttttctac accaatgat atcaaagtt 
ttgatcgat atcgtggtg cggaatgtg gccgatttg 
tcgccaggg gacaagcgg aggcaacag tacatcgaa 
ttgtgccgg ctggtgatg cggaacacg aactacgac 
gagcaccgg caccgcttg gacgacatt ctgaaaagc 
ttcactcgg attttctgc gaggagagc gagttgagc 
aaaaccgac cagcagctg gtccgtgag atttcgcac 
gagtttccg catttcttc aaataa
    mRNA sequence
    • >TC011794 RA
      acacgcatt tatcgactt ttccaacaa taaccaact 
tttacgcag ttttttcgc tggtaaaat aagataaat 
tccacttac ccaactgct gtcaattca aaacaaaca 
gttttttcg tacaaacag gcacaagaa tcaaatttg 
acgaaaacc ttgaaactc aatgtcgcc acccgtaaa 
aagtttcac caaatggat ttgatttca cagaaatac 
aacgtacaa tgaacccaa ggtacaatt ttcgatatt 
tgtgtgaga cgagggggt tggttttgg taacaattt 
gtataagta aacgtgaat taaaaaaat aaatttgaa 
tttgacgga tcgggtcct gcggctagt gcacgagcg 
agcacgatc caacaacat aaacaaaaa agaggcgtg 
acacattta tttgtcaaa acaataatt aatccaatt 
attactttc actttggcc gcttttacg taacaccac 
catgtcaca gaagatagt tttaacagg caccctcga 
catgctcca cagtctcta cgacttcaa agcaacgta 
cgcaaaaac cctcagttt tcgcaccaa cgaatactt 
catcctgca ccaaagcaa caccaagca caaaaactg 
gtgggaagt gataaacga agctgggga aatggggtt 
catcccctc gaactacgt cgagactgt cactgtgaa 
cccctcgtt ttacctcca gttcgtcga caactgtct 
cgagaacct gaagaagcg cgcgccggt ggagaacct 
caccgaccg ccacgaaat catcctgcg cttgaaaga 
agtgaagaa ggagatcga ggagctgcc cgaggtcgg 
gggtcacgg ggacgacct caagacgtt acattacaa 
aaactcgga tatggggcg gtttgagag catgagctc 
gcagtcttc gctcctaac cgaactaaa aaacgacat 
tgaggagcc catcaagcc tgtgaaagc gaacaaaga 
acgcgtgag gaagtccat cgaaaacat tcacgagga 
ggtgatttc cgagtgttg caacgacgt gaaaaaggc 
agagctgcc ctcgcccgc aatcacaga ccagtccgt 
ctacgagct ggttgagag tgtgaggat taatacgca 
gttgtctta cgaaatgtc gcgggtggc tgtggttac 
ggtcgttca ggggctgca cgagctgct gccagcgtc 
ggtgtttcc atacttaag cacgattct gtcacaagt 
acaaaaatc gctcaatgt ggatgacgt ccaaattga 
ccaaacgca cgatgctag tcgacttaa gatcatctt 
taatgagct cacttcgtg taaggagga ctcgcagca 
gaggagttg gatgcttca tgaagatga ggatgtgat 
caaagagta tctcttgga gttaatctc gattttgtc 
aaacgccga tgcgagcat ttctcgcca tgtcatttc 
ccgagacca ataccactg tatcgtcac cctgatcca 
gttttacca aatggaaat ccgctggtc gatccgcca 
gctcttact ccaaacgtt cggcgttct gtgcagtct 
ggacaaaac cgtcgtcag cataatgct caactcgat 
cctcccagg agagcttgc cagggatat gatggcgaa 
cgctcgcaa cattcccaa attaaacta ctcctccat 
tctcctcac catgatttt ctccatggg cgagcccat 
gcccatcac ccaccacga actactcgg ggcgaattt 
cttgtcatt cctcctcaa taacattga gtatccgcc 
cgatacgga catcgacga gcaaatccc cgatttgtt 
cctcaattg cgtcatttc gttcaattt gcagtttga 
ggactgtga cgaaaatcc ggtcctcag cgcgttaga 
ggagcgcga cgttgcgaa aaccttcac ggagaaaat 
cctcctcct cctcaaccg cgagcacga tccggttcg 
catttttga gcacgaacc ggcgccgcc tcacgccct 
cctcaagct ttttgtgga cttgctgag tcgcaaaac 
cactgctgc gcttttcta caccaatga tatcaaagt 
tttgatcga tatcgtggt gcggaatgt ggccgattt 
gtcgccagg ggacaagcg gaggcaaca gtacatcga 
attgtgccg gctggtgat gcggaacac gaactacga 
cgagcaccg gcaccgctt ggacgacat tctgaaaag 
cttcactcg gattttctg cgaggagag cgagttgag 
caaaaccga ccagcagct ggtccgtga gatttcgca 
cgagtttcc gcatttctt caaataatt ttgtgatac 
tttccgatt aaatcgatc aatgtgtta gcgtttttc 
attcctggg aattgtatg tgatatcct acctcacgt 
agtggtttt agggtcaac gcgcttgtg ttagtcgtg 
ccaaagaaa gcgacgatc gagtcaata aatttattt 
atgaaatga aatacaaat tgtaattaa aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY