| General information | |||
| TC011794 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 13457672..13463324 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC011794 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02932 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC011794 RA
MSQKIVLTG TLDMLHSLY DFKATYAKT LSFRTNEYF ILHQSNTKH KNWWEVINE AGEMGFIPS NYVETVTVN PSFYLQFVD NCLENLKKR APVENLTDR HEIILRLKE VKKEIEELP EVGGHGDDL KTLHYKNSD MGRFESMSS QSSLLTELK NDIEEPIKP VKANKERVR KSIENIHEE VISECCNDV KKAELPSPA ITDQSVYEL VESVRINTQ LSYEMSRVA VVTVVQGLH ELLPASVFP YLSTILSQV QKSLNVDDV QIDQTHDAS RLKIIFNEL TSCKEDSQQ RSWMLHEDE DVIKEYLLE LISILSNAD ASISRHVIS RDQYHCIVT LIQFYQMEI RWSIRQLLL QTFGVLCSL DKTVVSIML NSILPGELA RDMMANARN IPKLNYSSI LLTMIFSMG EPMPITHHE LLGANFLSF LLNNIEYPP DTDIDEQIP DLFLNCVIS FNLQFEDCD ENPVLSALE ERDVAKTFT EKILLLLNR EHDPVRIFE HEPAPPHAL LKLFVDLLS RKTTAALFY TNDIKVLID IVVRNVADL SPGDKRRQQ YIELCRLVM RNTNYDEHR HRLDDILKS FTRIFCEES ELSKTDQQL VREISHEFP HFFK
- >TC011794 RA
atgtcacag aagatagtt ttaacaggc accctcgac atgctccac agtctctac gacttcaaa gcaacgtac gcaaaaacc ctcagtttt cgcaccaac gaatacttc atcctgcac caaagcaac accaagcac aaaaactgg tgggaagtg ataaacgaa gctggggaa atggggttc atcccctcg aactacgtc gagactgtc actgtgaac ccctcgttt tacctccag ttcgtcgac aactgtctc gagaacctg aagaagcgc gcgccggtg gagaacctc accgaccgc cacgaaatc atcctgcgc ttgaaagaa gtgaagaag gagatcgag gagctgccc gaggtcggg ggtcacggg gacgacctc aagacgtta cattacaaa aactcggat atggggcgg tttgagagc atgagctcg cagtcttcg ctcctaacc gaactaaaa aacgacatt gaggagccc atcaagcct gtgaaagcg aacaaagaa cgcgtgagg aagtccatc gaaaacatt cacgaggag gtgatttcc gagtgttgc aacgacgtg aaaaaggca gagctgccc tcgcccgca atcacagac cagtccgtc tacgagctg gttgagagt gtgaggatt aatacgcag ttgtcttac gaaatgtcg cgggtggct gtggttacg gtcgttcag gggctgcac gagctgctg ccagcgtcg gtgtttcca tacttaagc acgattctg tcacaagta caaaaatcg ctcaatgtg gatgacgtc caaattgac caaacgcac gatgctagt cgacttaag atcatcttt aatgagctc acttcgtgt aaggaggac tcgcagcag aggagttgg atgcttcat gaagatgag gatgtgatc aaagagtat ctcttggag ttaatctcg attttgtca aacgccgat gcgagcatt tctcgccat gtcatttcc cgagaccaa taccactgt atcgtcacc ctgatccag ttttaccaa atggaaatc cgctggtcg atccgccag ctcttactc caaacgttc ggcgttctg tgcagtctg gacaaaacc gtcgtcagc ataatgctc aactcgatc ctcccagga gagcttgcc agggatatg atggcgaac gctcgcaac attcccaaa ttaaactac tcctccatt ctcctcacc atgattttc tccatgggc gagcccatg cccatcacc caccacgaa ctactcggg gcgaatttc ttgtcattc ctcctcaat aacattgag tatccgccc gatacggac atcgacgag caaatcccc gatttgttc ctcaattgc gtcatttcg ttcaatttg cagtttgag gactgtgac gaaaatccg gtcctcagc gcgttagag gagcgcgac gttgcgaaa accttcacg gagaaaatc ctcctcctc ctcaaccgc gagcacgat ccggttcgc atttttgag cacgaaccg gcgccgcct cacgccctc ctcaagctt tttgtggac ttgctgagt cgcaaaacc actgctgcg cttttctac accaatgat atcaaagtt ttgatcgat atcgtggtg cggaatgtg gccgatttg tcgccaggg gacaagcgg aggcaacag tacatcgaa ttgtgccgg ctggtgatg cggaacacg aactacgac gagcaccgg caccgcttg gacgacatt ctgaaaagc ttcactcgg attttctgc gaggagagc gagttgagc aaaaccgac cagcagctg gtccgtgag atttcgcac gagtttccg catttcttc aaataa
- >TC011794 RA
acacgcatt tatcgactt ttccaacaa taaccaact tttacgcag ttttttcgc tggtaaaat aagataaat tccacttac ccaactgct gtcaattca aaacaaaca gttttttcg tacaaacag gcacaagaa tcaaatttg acgaaaacc ttgaaactc aatgtcgcc acccgtaaa aagtttcac caaatggat ttgatttca cagaaatac aacgtacaa tgaacccaa ggtacaatt ttcgatatt tgtgtgaga cgagggggt tggttttgg taacaattt gtataagta aacgtgaat taaaaaaat aaatttgaa tttgacgga tcgggtcct gcggctagt gcacgagcg agcacgatc caacaacat aaacaaaaa agaggcgtg acacattta tttgtcaaa acaataatt aatccaatt attactttc actttggcc gcttttacg taacaccac catgtcaca gaagatagt tttaacagg caccctcga catgctcca cagtctcta cgacttcaa agcaacgta cgcaaaaac cctcagttt tcgcaccaa cgaatactt catcctgca ccaaagcaa caccaagca caaaaactg gtgggaagt gataaacga agctgggga aatggggtt catcccctc gaactacgt cgagactgt cactgtgaa cccctcgtt ttacctcca gttcgtcga caactgtct cgagaacct gaagaagcg cgcgccggt ggagaacct caccgaccg ccacgaaat catcctgcg cttgaaaga agtgaagaa ggagatcga ggagctgcc cgaggtcgg gggtcacgg ggacgacct caagacgtt acattacaa aaactcgga tatggggcg gtttgagag catgagctc gcagtcttc gctcctaac cgaactaaa aaacgacat tgaggagcc catcaagcc tgtgaaagc gaacaaaga acgcgtgag gaagtccat cgaaaacat tcacgagga ggtgatttc cgagtgttg caacgacgt gaaaaaggc agagctgcc ctcgcccgc aatcacaga ccagtccgt ctacgagct ggttgagag tgtgaggat taatacgca gttgtctta cgaaatgtc gcgggtggc tgtggttac ggtcgttca ggggctgca cgagctgct gccagcgtc ggtgtttcc atacttaag cacgattct gtcacaagt acaaaaatc gctcaatgt ggatgacgt ccaaattga ccaaacgca cgatgctag tcgacttaa gatcatctt taatgagct cacttcgtg taaggagga ctcgcagca gaggagttg gatgcttca tgaagatga ggatgtgat caaagagta tctcttgga gttaatctc gattttgtc aaacgccga tgcgagcat ttctcgcca tgtcatttc ccgagacca ataccactg tatcgtcac cctgatcca gttttacca aatggaaat ccgctggtc gatccgcca gctcttact ccaaacgtt cggcgttct gtgcagtct ggacaaaac cgtcgtcag cataatgct caactcgat cctcccagg agagcttgc cagggatat gatggcgaa cgctcgcaa cattcccaa attaaacta ctcctccat tctcctcac catgatttt ctccatggg cgagcccat gcccatcac ccaccacga actactcgg ggcgaattt cttgtcatt cctcctcaa taacattga gtatccgcc cgatacgga catcgacga gcaaatccc cgatttgtt cctcaattg cgtcatttc gttcaattt gcagtttga ggactgtga cgaaaatcc ggtcctcag cgcgttaga ggagcgcga cgttgcgaa aaccttcac ggagaaaat cctcctcct cctcaaccg cgagcacga tccggttcg catttttga gcacgaacc ggcgccgcc tcacgccct cctcaagct ttttgtgga cttgctgag tcgcaaaac cactgctgc gcttttcta caccaatga tatcaaagt tttgatcga tatcgtggt gcggaatgt ggccgattt gtcgccagg ggacaagcg gaggcaaca gtacatcga attgtgccg gctggtgat gcggaacac gaactacga cgagcaccg gcaccgctt ggacgacat tctgaaaag cttcactcg gattttctg cgaggagag cgagttgag caaaaccga ccagcagct ggtccgtga gatttcgca cgagtttcc gcatttctt caaataatt ttgtgatac tttccgatt aaatcgatc aatgtgtta gcgtttttc attcctggg aattgtatg tgatatcct acctcacgt agtggtttt agggtcaac gcgcttgtg ttagtcgtg ccaaagaaa gcgacgatc gagtcaata aatttattt atgaaatga aatacaaat tgtaattaa aa
