Status
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Gene information
General information
TC011794 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13457672..13463324
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011794
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02932

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011794 RA
      MSQKIVLTG TLDMLHSLY DFKATYAKT LSFRTNEYF 
      ILHQSNTKH KNWWEVINE AGEMGFIPS NYVETVTVN 
      PSFYLQFVD NCLENLKKR APVENLTDR HEIILRLKE 
      VKKEIEELP EVGGHGDDL KTLHYKNSD MGRFESMSS 
      QSSLLTELK NDIEEPIKP VKANKERVR KSIENIHEE 
      VISECCNDV KKAELPSPA ITDQSVYEL VESVRINTQ 
      LSYEMSRVA VVTVVQGLH ELLPASVFP YLSTILSQV 
      QKSLNVDDV QIDQTHDAS RLKIIFNEL TSCKEDSQQ 
      RSWMLHEDE DVIKEYLLE LISILSNAD ASISRHVIS 
      RDQYHCIVT LIQFYQMEI RWSIRQLLL QTFGVLCSL 
      DKTVVSIML NSILPGELA RDMMANARN IPKLNYSSI 
      LLTMIFSMG EPMPITHHE LLGANFLSF LLNNIEYPP 
      DTDIDEQIP DLFLNCVIS FNLQFEDCD ENPVLSALE 
      ERDVAKTFT EKILLLLNR EHDPVRIFE HEPAPPHAL 
      LKLFVDLLS RKTTAALFY TNDIKVLID IVVRNVADL 
      SPGDKRRQQ YIELCRLVM RNTNYDEHR HRLDDILKS 
      FTRIFCEES ELSKTDQQL VREISHEFP HFFK
    CDS sequence
    • >TC011794 RA
      atgtcacag aagatagtt ttaacaggc accctcgac 
      atgctccac agtctctac gacttcaaa gcaacgtac 
      gcaaaaacc ctcagtttt cgcaccaac gaatacttc 
      atcctgcac caaagcaac accaagcac aaaaactgg 
      tgggaagtg ataaacgaa gctggggaa atggggttc 
      atcccctcg aactacgtc gagactgtc actgtgaac 
      ccctcgttt tacctccag ttcgtcgac aactgtctc 
      gagaacctg aagaagcgc gcgccggtg gagaacctc 
      accgaccgc cacgaaatc atcctgcgc ttgaaagaa 
      gtgaagaag gagatcgag gagctgccc gaggtcggg 
      ggtcacggg gacgacctc aagacgtta cattacaaa 
      aactcggat atggggcgg tttgagagc atgagctcg 
      cagtcttcg ctcctaacc gaactaaaa aacgacatt 
      gaggagccc atcaagcct gtgaaagcg aacaaagaa 
      cgcgtgagg aagtccatc gaaaacatt cacgaggag 
      gtgatttcc gagtgttgc aacgacgtg aaaaaggca 
      gagctgccc tcgcccgca atcacagac cagtccgtc 
      tacgagctg gttgagagt gtgaggatt aatacgcag 
      ttgtcttac gaaatgtcg cgggtggct gtggttacg 
      gtcgttcag gggctgcac gagctgctg ccagcgtcg 
      gtgtttcca tacttaagc acgattctg tcacaagta 
      caaaaatcg ctcaatgtg gatgacgtc caaattgac 
      caaacgcac gatgctagt cgacttaag atcatcttt 
      aatgagctc acttcgtgt aaggaggac tcgcagcag 
      aggagttgg atgcttcat gaagatgag gatgtgatc 
      aaagagtat ctcttggag ttaatctcg attttgtca 
      aacgccgat gcgagcatt tctcgccat gtcatttcc 
      cgagaccaa taccactgt atcgtcacc ctgatccag 
      ttttaccaa atggaaatc cgctggtcg atccgccag 
      ctcttactc caaacgttc ggcgttctg tgcagtctg 
      gacaaaacc gtcgtcagc ataatgctc aactcgatc 
      ctcccagga gagcttgcc agggatatg atggcgaac 
      gctcgcaac attcccaaa ttaaactac tcctccatt 
      ctcctcacc atgattttc tccatgggc gagcccatg 
      cccatcacc caccacgaa ctactcggg gcgaatttc 
      ttgtcattc ctcctcaat aacattgag tatccgccc 
      gatacggac atcgacgag caaatcccc gatttgttc 
      ctcaattgc gtcatttcg ttcaatttg cagtttgag 
      gactgtgac gaaaatccg gtcctcagc gcgttagag 
      gagcgcgac gttgcgaaa accttcacg gagaaaatc 
      ctcctcctc ctcaaccgc gagcacgat ccggttcgc 
      atttttgag cacgaaccg gcgccgcct cacgccctc 
      ctcaagctt tttgtggac ttgctgagt cgcaaaacc 
      actgctgcg cttttctac accaatgat atcaaagtt 
      ttgatcgat atcgtggtg cggaatgtg gccgatttg 
      tcgccaggg gacaagcgg aggcaacag tacatcgaa 
      ttgtgccgg ctggtgatg cggaacacg aactacgac 
      gagcaccgg caccgcttg gacgacatt ctgaaaagc 
      ttcactcgg attttctgc gaggagagc gagttgagc 
      aaaaccgac cagcagctg gtccgtgag atttcgcac 
      gagtttccg catttcttc aaataa
    mRNA sequence
    • >TC011794 RA
      acacgcatt tatcgactt ttccaacaa taaccaact 
      tttacgcag ttttttcgc tggtaaaat aagataaat 
      tccacttac ccaactgct gtcaattca aaacaaaca 
      gttttttcg tacaaacag gcacaagaa tcaaatttg 
      acgaaaacc ttgaaactc aatgtcgcc acccgtaaa 
      aagtttcac caaatggat ttgatttca cagaaatac 
      aacgtacaa tgaacccaa ggtacaatt ttcgatatt 
      tgtgtgaga cgagggggt tggttttgg taacaattt 
      gtataagta aacgtgaat taaaaaaat aaatttgaa 
      tttgacgga tcgggtcct gcggctagt gcacgagcg 
      agcacgatc caacaacat aaacaaaaa agaggcgtg 
      acacattta tttgtcaaa acaataatt aatccaatt 
      attactttc actttggcc gcttttacg taacaccac 
      catgtcaca gaagatagt tttaacagg caccctcga 
      catgctcca cagtctcta cgacttcaa agcaacgta 
      cgcaaaaac cctcagttt tcgcaccaa cgaatactt 
      catcctgca ccaaagcaa caccaagca caaaaactg 
      gtgggaagt gataaacga agctgggga aatggggtt 
      catcccctc gaactacgt cgagactgt cactgtgaa 
      cccctcgtt ttacctcca gttcgtcga caactgtct 
      cgagaacct gaagaagcg cgcgccggt ggagaacct 
      caccgaccg ccacgaaat catcctgcg cttgaaaga 
      agtgaagaa ggagatcga ggagctgcc cgaggtcgg 
      gggtcacgg ggacgacct caagacgtt acattacaa 
      aaactcgga tatggggcg gtttgagag catgagctc 
      gcagtcttc gctcctaac cgaactaaa aaacgacat 
      tgaggagcc catcaagcc tgtgaaagc gaacaaaga 
      acgcgtgag gaagtccat cgaaaacat tcacgagga 
      ggtgatttc cgagtgttg caacgacgt gaaaaaggc 
      agagctgcc ctcgcccgc aatcacaga ccagtccgt 
      ctacgagct ggttgagag tgtgaggat taatacgca 
      gttgtctta cgaaatgtc gcgggtggc tgtggttac 
      ggtcgttca ggggctgca cgagctgct gccagcgtc 
      ggtgtttcc atacttaag cacgattct gtcacaagt 
      acaaaaatc gctcaatgt ggatgacgt ccaaattga 
      ccaaacgca cgatgctag tcgacttaa gatcatctt 
      taatgagct cacttcgtg taaggagga ctcgcagca 
      gaggagttg gatgcttca tgaagatga ggatgtgat 
      caaagagta tctcttgga gttaatctc gattttgtc 
      aaacgccga tgcgagcat ttctcgcca tgtcatttc 
      ccgagacca ataccactg tatcgtcac cctgatcca 
      gttttacca aatggaaat ccgctggtc gatccgcca 
      gctcttact ccaaacgtt cggcgttct gtgcagtct 
      ggacaaaac cgtcgtcag cataatgct caactcgat 
      cctcccagg agagcttgc cagggatat gatggcgaa 
      cgctcgcaa cattcccaa attaaacta ctcctccat 
      tctcctcac catgatttt ctccatggg cgagcccat 
      gcccatcac ccaccacga actactcgg ggcgaattt 
      cttgtcatt cctcctcaa taacattga gtatccgcc 
      cgatacgga catcgacga gcaaatccc cgatttgtt 
      cctcaattg cgtcatttc gttcaattt gcagtttga 
      ggactgtga cgaaaatcc ggtcctcag cgcgttaga 
      ggagcgcga cgttgcgaa aaccttcac ggagaaaat 
      cctcctcct cctcaaccg cgagcacga tccggttcg 
      catttttga gcacgaacc ggcgccgcc tcacgccct 
      cctcaagct ttttgtgga cttgctgag tcgcaaaac 
      cactgctgc gcttttcta caccaatga tatcaaagt 
      tttgatcga tatcgtggt gcggaatgt ggccgattt 
      gtcgccagg ggacaagcg gaggcaaca gtacatcga 
      attgtgccg gctggtgat gcggaacac gaactacga 
      cgagcaccg gcaccgctt ggacgacat tctgaaaag 
      cttcactcg gattttctg cgaggagag cgagttgag 
      caaaaccga ccagcagct ggtccgtga gatttcgca 
      cgagtttcc gcatttctt caaataatt ttgtgatac 
      tttccgatt aaatcgatc aatgtgtta gcgtttttc 
      attcctggg aattgtatg tgatatcct acctcacgt 
      agtggtttt agggtcaac gcgcttgtg ttagtcgtg 
      ccaaagaaa gcgacgatc gagtcaata aatttattt 
      atgaaatga aatacaaat tgtaattaa aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY