Status
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Gene information
General information
TC031247
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011812,TC011810
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13253486..13280800
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031247
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02931

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands content darker - Number of animals showing the phenotype: 4 (41 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031247 RA
      MLGFSTSRD GLLQLSEEQ LAAVVHKEP ILDHYGVDK 
      TPLGRGKYA TVCRAVHKE TGVEYAAKF VKKRRRNQD 
      QMKEIIHEI AVLMQCSST NRVIRLHEV YESATEMVL 
      VLELAAGGE LQHILDGGQ CLGEAEARK AMKQILDGV 
      SFLHERNIV HLDLKPQNL LLSIEDNCD DIKLCDFGI 
      SKVLEPGVK VREIIGTVD YVAPEVLSY DPICLSTDI 
      WSIGVLAYV LLSGYTPFG ADDKQQTFL NISKCALSF 
      EPDHFEDVS SPAIDFIKS ALVTDPRKR PTVHELLEH 
      PWISLKCPL LPALPLKPS ESVPSIKSI SETPKSQRK 
      TFCSDALSG SLSTFTVSS NCLCPQCGT ACRHLTHTP 
      VPKTAITVD RGILC
    CDS sequence
    • >TC031247 RA
      atgctggga tttagtact tcacgcgac ggccttctg 
      cagctgtcg gaggaacag ctggcggcc gttgtgcac 
      aaggagccg attttggac cattacggg gtggataag 
      acaccactt ggcaggggc aaatacgcg acggtgtgt 
      cgcgccgtc cacaaggag actggtgtc gaatacgcg 
      gcgaaattc gtgaagaaa cgccgccgt aaccaggac 
      cagatgaag gagatcatc cacgagatt gccgtttta 
      atgcagtgc tcgtcgacg aatcgcgtg attcgtctg 
      cacgaggtc tacgaatcg gccacggag atggtgttg 
      gtgctggaa ttggccgcc ggcggcgag ctccagcac 
      atcctcgac ggcggccag tgtctgggc gaggcggag 
      gcgcgcaaa gccatgaag cagatcctg gacggagtg 
      tcgtttctc cacgaaagg aacatcgtt catttggac 
      ttaaaaccg cagaattta ctgctctcg atcgaggat 
      aactgtgat gatatcaag ctctgtgat ttcggcata 
      tcgaaggtt ttggagccc ggcgttaaa gtacgggag 
      attataggt actgtagat tatgtagcg ccggaagtg 
      cttagttac gacccgatc tgtctgtcg actgatatt 
      tggtcgatt ggagttttg gcgtatgtg cttttgtcg 
      ggctatacg ccgtttggg gccgacgat aagcaacag 
      acttttctt aatatatca aagtgtgcc ttatctttc 
      gagccagac catttcgag gatgtttcc tcgccggct 
      atagacttt atcaagagt gccttggtc acggatccc 
      aggaagcgt ccgaccgtc cacgagctg ttggagcac 
      ccgtggatt tcgctgaag tgccccctc ctgccggcg 
      ctgccgctg aagccttcg gagtccgtt ccgtcgata 
      aaaagcatc tcggagacg ccgaaatcg cagcggaaa 
      acattttgc tcagacgct ttaagcgga agcttaagt 
      acgttcacg gtatcgagt aactgcctt tgtccgcaa 
      tgtggaacg gcttgccgt catctgaca cacacacca 
      gtgcccaaa accgctatc accgtcgat agaggcatt 
      ctatgttag
    mRNA sequence
    • >TC031247 RA
      aaattgttt gctccagtc gttcagttt gaagaggac 
      atagcaagt gtacacaag tttgttacc ttgagtgtg 
      ttcatgttg tatgataag tgtccccgc gttatgtgg 
      ccccaaaca gttatcatg taaacgcgt ttttctgtt 
      aatcgaatc gatcaagtc gcctgccct attgttatt 
      tctcaagga tcgcgtatc ctcaggtag tgaggacct 
      ggctagttt tttccgtcg tgtcaaata aaacgtttc 
      aaatttttg cgatttagt gtgtgttaa ggacttttg 
      gagcgaaat gctgggatt tagtacttc acgcgacgg 
      ccttctgca gctgtcgga ggaacagct ggcggccgt 
      tgtgcacaa ggagccgat tttggacca ttacggggt 
      ggataagac accacttgg caggggcaa atacgcgac 
      ggtgtgtcg cgccgtcca caaggagac tggtgtcga 
      atacgcggc gaaattcgt gaagaaacg ccgccgtaa 
      ccaggacca gatgaagga gatcatcca cgagattgc 
      cgttttaat gcagtgctc gtcgacgaa tcgcgtgat 
      tcgtctgca cgaggtcta cgaatcggc cacggagat 
      ggtgttggt gctggaatt ggccgccgg cggcgagct 
      ccagcacat cctcgacgg cggccagtg tctgggcga 
      ggcggaggc gcgcaaagc catgaagca gatcctgga 
      cggagtgtc gtttctcca cgaaaggaa catcgttca 
      tttggactt aaaaccgca gaatttact gctctcgat 
      cgaggataa ctgtgatga tatcaagct ctgtgattt 
      cggcatatc gaaggtttt ggagcccgg cgttaaagt 
      acgggagat tataggtac tgtagatta tgtagcgcc 
      ggaagtgct tagttacga cccgatctg tctgtcgac 
      tgatatttg gtcgattgg agttttggc gtatgtgct 
      tttgtcggg ctatacgcc gtttggggc cgacgataa 
      gcaacagac ttttcttaa tatatcaaa gtgtgcctt 
      atctttcga gccagacca tttcgagga tgtttcctc 
      gccggctat agactttat caagagtgc cttggtcac 
      ggatcccag gaagcgtcc gaccgtcca cgagctgtt 
      ggagcaccc gtggatttc gctgaagtg ccccctcct 
      gccggcgct gccgctgaa gccttcgga gtccgttcc 
      gtcgataaa aagcatctc ggagacgcc gaaatcgca 
      gcggaaaac attttgctc agacgcttt aagcggaag 
      cttaagtac gttcacggt atcgagtaa ctgcctttg 
      tccgcaatg tggaacggc ttgccgtca tctgacaca 
      cacaccagt gcccaaaac cgctatcac cgtcgatag 
      aggcattct atgttagcc aaagttact ttgtttgtg 
      cccgaagag gttgcgaaa caagccgat tgtttcatg 
      tgcttgggt tatgtacat ttgtttagt ttaagttac 
      gataattta tgttttatt gcttctttt ttgtttatg 
      gttagtgta cctaaataa agtattctt ttttgttgt 
      gtatattct ttagtttga ctttctgat cattgcaga 
      atcaagttt cggactgat ttatacaac tagaggcta 
      gtcacagtt ttgcttgtt gtgcccaaa ccaatcaaa 
      ttttagcac aaagtgcag tgttgattt tgtaacgag 
      tctactcag gagaatttg tgttgagca caaatcaga 
      atttattgt atgtttgga cggttgggg cggcaagta 
      gggctaggg tagttgccg ccccacaaa tatttattg 
      ttcggatta atgttgtgt gttgtgaag gtctcatat 
      cactagttg tacgcaaat cttgacaat actcagtct 
      tttctttgc aggagagat ggacgacgg cgttgtaat 
      aacctgatc acgactttc agtaatttt aagttagca 
      ataaaattt gattcgaac t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY