Status
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Gene information
General information
TC034431
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011818,TC012684
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13119730..13143558
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034431
    iBeetle screen iB_02930

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber late shape irregular - Number of animals showing the phenotype: 3

    mature egg not present - Number of animals showing the phenotype: 3
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034431 RA
      MSRLLFQIT LCNPKVLGN SYSPTLLLK QHVYFTLPA 
      ATYVNKSDR KATTVALTE DVTKAFPEK PSIIPAWRP 
      TPSTDRSNI IQHYLKLSK IRLTTLVVV TSMAGYAVA 
      PAPFEWGPF ALCIAGTGL LSAAANAVN QFHEVPFDA 
      QMNRTKHRV LVCGRLTPL HSILFAATT SLSGLAILN 
      FGVNGLTAT LGLTNFILY TSIYTPMKR MSILNTWVG 
      SIVGAIPPL MGWAACANS LGPGAWLMA ALLYSWQFP 
      HFNALSWNL RPDYSRAGY RMMAVTDPG LCRRVALRH 
      TVALEAMTI AAPFLDVTN WWFVLEASP LNLYFIYLA 
      YDFYKDSSS QSSRKLFRF SLIHLPLLM ILFLVNKKK 
      WFVFDEDTE RSCSVKNVE TRREPSRAP VRGHSLERH 
      VPIESRGAF PAVVDLFTT FSRKLAEVK RESEPEWCD 
      AAATATADF HEETYAATD DDYRMVEAA PPRHHHQLL 
      GNVFGEGFK PVFKRGATP ATATSLFTI DSILAPRPK 
      PSSPQRPVL QHPGLHLGH IAAAGGFGA SSADFLAMA 
      YPGLYPGYV HALAAASQA ASMAGSHPQ HQPPKRKRR 
      HRTIFSEEQ LEQLEATFE QTHYPDVVL REQLALKVD 
      LKEERVEVW FKNRRAKWR KQKREEQER MRKIQEEDV 
      CRSVEQQRL LQPQNFSDE DSSDLEVA
    CDS sequence
    • >TC034431 RA
      atgtcaaga ctattgttc caaatcaca ttatgcaac 
      ccgaaagtg ttaggtaat tcatacagc cccactttg 
      ctcctcaag caacacgtc tacttcacg ttacctgca 
      gccacttat gtaaataaa tccgaccgg aaggcgacc 
      acagtcgcc ctcactgaa gacgtcacc aaagcattt 
      cccgaaaaa ccatcgata atcccagca tggcggccc 
      acgccttcc accgaccgt tcaaacatc atccaacac 
      tacctcaaa ctgtccaaa atccgcctc acaaccctt 
      gttgtggtc acctcgatg gcgggctac gccgtcgcc 
      cccgcgccc ttcgaatgg gggcccttt gccctgtgc 
      attgccggg acggggctg ttgtcggcc gccgctaac 
      gccgtcaac cagttccat gaagtcccg tttgacgca 
      caaatgaac aggaccaag catcgggtg ctagtctgc 
      ggccgcttg acccccctt cattcaatc ttatttgcc 
      gcaactaca agtttgagt gggttggcc attttgaat 
      tttggggtt aatggactt actgccact ttggggcta 
      actaatttc atcctctat acgtcgatt tacacccca 
      atgaagcgg atgagcatc ctgaacacg tgggtgggc 
      tcaatcgtt ggtgccatt cccccgttg atgggttgg 
      gccgcctgt gccaattcc ctggggccg ggagcgtgg 
      ctcatggcg gcccttttg tacagttgg caattcccg 
      catttcaac gccttgtcc tggaatttg cgcccggat 
      tactccagg gccggctac cggatgatg gcggtcaca 
      gatccgggc ttgtgtcga cgagtggcc ctgagacat 
      accgtagcc cttgaggcg atgacgata gcagcgccc 
      tttttagac gttaccaac tggtggttc gttttagaa 
      gcctcaccg ttgaatttg tattttatt tatttagcg 
      tatgatttt tacaaagat tcgtccagt cagagctcc 
      cggaagttg ttccggttt tcgttgatt cacttgccg 
      ttgttgatg attttgttt ctagtaaat aaaaagaag 
      tggttcgtt ttcgatgaa gacaccgaa cgtagttgt 
      agtgtaaaa aatgtagaa actcgcagg gagccctcg 
      cgggccccc gtcaggggc cactcattg gagcggcac 
      gtaccaatc gaatcgagg ggggcgttc cccgccgtc 
      gtcgacttg ttcaccact ttttcccga aaactggcg 
      gaagtcaaa cgcgaaagt gagccggag tggtgcgac 
      gccgccgcc actgccacc gccgatttt cacgaggag 
      acgtacgcc gccaccgac gacgactac aggatggtg 
      gaggcggcg ccgccgagg caccaccac cagctgctg 
      gggaacgtc ttcggagag ggcttcaag ccggtgttt 
      aaacgagga gcgacgccg gcgacggcc acctcgctt 
      tttaccatc gatagcatt ctggcgccg aggccgaag 
      ccgagcagt ccgcagagg ccggtgctc cagcacccg 
      ggcctgcat ctgggacat atcgccgcc gccgggggg 
      tttggcgcc tcctcggcg gattttcta gcgatggcg 
      tatcctggg ctgtacccc ggatacgtg cacgcgctg 
      gcggcggca tcgcaggcc gcctccatg gcgggcagc 
      cacccccag caccagccc ccgaaacga aaacgtcgc 
      caccgcacc atcttcagc gaggagcag ctggagcag 
      ctggaggcg acgttcgag caaacgcat tatcccgac 
      gtcgtcctc agagagcag ctcgctctc aaagtggat 
      ttgaaggag gagcgggtc gaggtctgg ttcaaaaac 
      cgaagagcc aagtggcgg aagcagaag cgcgaagag 
      caggagcgg atgaggaag attcaggag gaggatgtc 
      tgcaggagt gtggagcag cagagactc ctgcagccg 
      cagaacttc agcgacgag gattcgagc gacctggag 
      gtggcttga
    mRNA sequence
    • >TC034431 RA
      aaaaccacc tgactgacc aaatgtcaa aaccttgac 
      ctaacctaa aaatcacgc aataattcc gccaaaaac 
      cgtaaaaaa tgcccaaac tggcgtgat ataatgtca 
      agactattg ttccaaatc acattatgc aacccgaaa 
      gtgttaggt aattcatac agccccact ttgctcctc 
      aagcaacac gtctacttc acgttacct gcagccact 
      tatgtaaat aaatccgac cggaaggcg accacagtc 
      gccctcact gaagacgtc accaaagca tttcccgaa 
      aaaccatcg ataatccca gcatggcgg cccacgcct 
      tccaccgac cgttcaaac atcatccaa cactacctc 
      aaactgtcc aaaatccgc ctcacaacc cttgttgtg 
      gtcacctcg atggcgggc tacgccgtc gcccccgcg 
      cccttcgaa tgggggccc tttgccctg tgcattgcc 
      gggacgggg ctgttgtcg gccgccgct aacgccgtc 
      aaccagttc catgaagtc ccgtttgac gcacaaatg 
      aacaggacc aagcatcgg gtgctagtc tgcggccgc 
      ttgaccccc cttcattca atcttattt gccgcaact 
      acaagtttg agtgggttg gccattttg aattttggg 
      gttaatgga cttactgcc actttgggg ctaactaat 
      ttcatcctc tatacgtcg atttacacc ccaatgaag 
      cggatgagc atcctgaac acgtgggtg ggctcaatc 
      gttggtgcc attcccccg ttgatgggt tgggccgcc 
      tgtgccaat tccctgggg ccgggagcg tggctcatg 
      gcggccctt ttgtacagt tggcaattc ccgcatttc 
      aacgccttg tcctggaat ttgcgcccg gattactcc 
      agggccggc taccggatg atggcggtc acagatccg 
      ggcttgtgt cgacgagtg gccctgaga cataccgta 
      gcccttgag gcgatgacg atagcagcg cccttttta 
      gacgttacc aactggtgg ttcgtttta gaagcctca 
      ccgttgaat ttgtatttt atttattta gcgtatgat 
      ttttacaaa gattcgtcc agtcagagc tcccggaag 
      ttgttccgg ttttcgttg attcacttg ccgttgttg 
      atgattttg tttctagta aataaaaag aagtggttc 
      gttttcgat gaagacacc gaacgtagt tgtagtgta 
      aaaaatgta gaaactcgc agggagccc tcgcgggcc 
      cccgtcagg ggccactca ttggagcgg cacgtacca 
      atcgaatcg aggggggcg ttccccgcc gtcgtcgac 
      ttgttcacc actttttcc cgaaaactg gcggaagtc 
      aaacgcgaa agtgagccg gagtggtgc gacgccgcc 
      gccactgcc accgccgat tttcacgag gagacgtac 
      gccgccacc gacgacgac tacaggatg gtggaggcg 
      gcgccgccg aggcaccac caccagctg ctggggaac 
      gtcttcgga gagggcttc aagccggtg tttaaacga 
      ggagcgacg ccggcgacg gccacctcg ctttttacc 
      atcgatagc attctggcg ccgaggccg aagccgagc 
      agtccgcag aggccggtg ctccagcac ccgggcctg 
      catctggga catatcgcc gccgccggg gggtttggc 
      gcctcctcg gcggatttt ctagcgatg gcgtatcct 
      gggctgtac cccggatac gtgcacgcg ctggcggcg 
      gcatcgcag gccgcctcc atggcgggc agccacccc 
      cagcaccag cccccgaaa cgaaaacgt cgccaccgc 
      accatcttc agcgaggag cagctggag cagctggag 
      gcgacgttc gagcaaacg cattatccc gacgtcgtc 
      ctcagagag cagctcgct ctcaaagtg gatttgaag 
      gaggagcgg gtcgaggtc tggttcaaa aaccgaaga 
      gccaagtgg cggaagcag aagcgcgaa gagcaggag 
      cggatgagg aagattcag gaggaggat gtctgcagg 
      agtgtggag cagcagaga ctcctgcag ccgcagaac 
      ttcagcgac gaggattcg agcgacctg gaggtggct 
      tgaaagagg gaggagggg ttttatcta gtcaattgt 
      tgctgttct gtataaatg tatattgtt tggggttta 
      ctacatttt gtgcaaata tcggcggac ggaaggttc 
      cgagatttg agttgcggt caggttgtt tcggttcgt 
      gtaaatatt aggttaaga tgagttatg atcaagttg 
      ttgtatttt aaattacgt atcgacagg ttagactat 
      tgtgcatgc aaataccta tttaataac tgtaacata 
      tgaggaaat aaatatcat tttcgtaa
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